Rmu_sc0002448.1_g000008

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002448.1
OG0010590
Physical Location & Seq
Forward (+)
25232 .. 26524
1293 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002448.1_g000008.1.cds

Sequence Viewer

Length: 1293 bp
atgtcgtatagatcgaaattcccattaaagagcaagagcaagaagaatgtggatgaagaagacaaggcggaaaaaggaagaaaagagactattcagtgctctctcttggtggctgcaaggaagctattgatgagcaatctgagccaggaaaagaaagccttcattcccgacgatcatcgctctctgtacctctgcttcggtgactacgaagatggcagcaaggtctcctacaccattctatcactcagcttgcgccaactgttctctgcttcatcttctccacagaaattacagctggttactaggttatccgacgatggtcttcctcgttatatgggttgcggcgtttggggctccaaaattcttctcgggggtggcgcaaaaccgggttatttccataatccgacacgagggcatcccattactgggtgtttcaagcatcaccaagtcagaaagatctactgttttgagacaacagaagagactcaagtgccggtgccgctccctaagaagatgctgaatgggaacatccagccccggctggtggaggtggacaacaaattatacgttattggtacatcattatcttcctttgagatgtttgatggcaatgattggtctgaattggcaccacctccgatcagatactatttctgttatccatttacacactcagacccggcttgtcacttcgatatatattatgcagttgcaagcacaaacatattggtttcttctggtggttcaagcttccgctttgacacagctgaccccaaccaaggctggagaaaactaatgtgggctgatcaattctctttcaatcaattctattttaagggcacatgtttggttgtgaatgatggtaagcaatttgtgcttttcacattccaaccctccgatggtcatcacatatcagtgcatctgatttcatatgacttcagtagcatcacacaagactacagtcaagtgcatctgccttcgttcttaatggaatgcaagcgtgatcgtcttggtgatttttcattacccaattatcggctggtacacctaggagataaggtcgtctgccttcttatgtctgaacttcgccccagtggagtggatgagaggaagaagaagatgattagatctgttgaatttgccgcattcacattcaaagtcaaagacaatgctaagcccttgaaaatcagatttctacctcgtactccaaagctcatatatgatgatgacaatgatgatgatatggacgtggcttttagggctggacagatgcttggtgccttcttgctgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

430

Amino Acids

49.03

Weight (kDa)

8.72

Isoelectric Point (pI)

40.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 496, 628, 1277
AccBSI CCGCTC 1 cut(s) 502
AciI CCGC 5 cut(s) 68, 342, 500, 754, 1143
AcsI RAATTY 3 cut(s) 17, 360, 1136
AcuI CTGAAG 1 cut(s) 922
AfaI GTAC 4 cut(s) 188, 577, 1044, 1204
AfiI CCNNNNNNNGG 7 cut(s) 410, 425, 426, 544, 779, 899, 1035
AflIII ACRYGT 1 cut(s) 842
AgsI TTSAA 6 cut(s) 436, 747, 820, 1136, 1156, 1183
AjiI CACGTC 1 cut(s) 1249
AjnI CCWGG 1 cut(s) 144
AluBI AGCT 6 cut(s) 124, 249, 295, 750, 767, 1213
AluI AGCT 6 cut(s) 124, 249, 295, 750, 767, 1213
Alw21I GWGCWC 1 cut(s) 101
Alw26I GTCTC 4 cut(s) 80, 229, 464, 476
Ama87I CYCGRG 1 cut(s) 368
ApeKI GCWGC 2 cut(s) 113, 216
ApoI RAATTY 3 cut(s) 17, 360, 1136
Asp700I GAANNNNTTC 1 cut(s) 158
AspA2I CCTAGG 1 cut(s) 1048
AspLEI GCGC 2 cut(s) 255, 380
AsuC2I CCSGG 3 cut(s) 387, 538, 680
AsuHPI GGTGA 3 cut(s) 212, 434, 1025
AvaI CYCGRG 1 cut(s) 368
AvrII CCTAGG 1 cut(s) 1048
BaeGI GKGCMC 1 cut(s) 842
BanI GGYRCC 3 cut(s) 496, 628, 1277
BanII GRGCYC 1 cut(s) 356
BarI GAAGNNNNNNTAC 2 cut(s) 179, 211
BauI CACGAG 1 cut(s) 408
BbsI GAAGAC 2 cut(s) 66, 314
Bbv12I GWGCWC 1 cut(s) 101
BbvI GCAGC 2 cut(s) 100, 228
BccI CCATC 5 cut(s) 206, 311, 599, 854, 893
BciT130I CCWGG 1 cut(s) 146
BclI TGATCA 1 cut(s) 805
BcnI CCSGG 3 cut(s) 387, 538, 680
BcoDI GTCTC 4 cut(s) 80, 229, 464, 476
BfaI CTAG 2 cut(s) 303, 1049
BfmI CTRYAG 1 cut(s) 958
BglII AGATCT 2 cut(s) 456, 1127
BisI GCNGC 5 cut(s) 114, 217, 343, 500, 1143
BlnI CCTAGG 1 cut(s) 1048
BlpI GCTNAGC 1 cut(s) 1173
BlsI GCNGC 5 cut(s) 115, 218, 344, 501, 1144
Bme1390I CCNGG 4 cut(s) 146, 387, 538, 680
BmeT110I CYCGRG 1 cut(s) 368
BmgBI CACGTC 1 cut(s) 1249
BmiI GGNNCC 4 cut(s) 355, 498, 630, 1279
BmrFI CCNGG 4 cut(s) 146, 387, 538, 680
BmrI ACTGGG 2 cut(s) 435, 1086
BmsI GCATC 7 cut(s) 424, 448, 504, 928, 954, 979, 1260
BmuI ACTGGG 2 cut(s) 435, 1086
BoxI GACNNNNGTC 2 cut(s) 318, 960
BpiI GAAGAC 2 cut(s) 66, 314
BpmI CTGGAG 1 cut(s) 805
Bpu1102I GCTNAGC 1 cut(s) 1173
BpuEI CTTGAG 1 cut(s) 471
BpuMI CCSGG 3 cut(s) 387, 538, 680
BsaBI GATNNNNATC 1 cut(s) 903
BsaI GGTCTC 1 cut(s) 229
BsaJI CCNNGG 3 cut(s) 536, 778, 1048
BsaXI ACNNNNNCTCC 2 cut(s) 1044, 1074
Bsc4I CCNNNNNNNGG 7 cut(s) 410, 425, 426, 544, 779, 899, 1035
Bse118I RCCGGY 1 cut(s) 493
Bse1I ACTGG 2 cut(s) 430, 1092
Bse3DI GCAATG 1 cut(s) 616
Bse8I GATNNNNATC 1 cut(s) 903
BseBI CCWGG 1 cut(s) 146
BseDI CCNNGG 3 cut(s) 536, 778, 1048
BseGI GGATG 4 cut(s) 58, 415, 528, 1108
BseJI GATNNNNATC 1 cut(s) 903
BseLI CCNNNNNNNGG 7 cut(s) 410, 425, 426, 544, 779, 899, 1035
BseMI GCAATG 1 cut(s) 616
BseMII CTCAG 3 cut(s) 131, 259, 687
BseNI ACTGG 2 cut(s) 430, 1092
BseSI GKGCMC 1 cut(s) 842
BseXI GCAGC 2 cut(s) 100, 228
BshNI GGYRCC 3 cut(s) 496, 628, 1277
BsiHKAI GWGCWC 1 cut(s) 101
BsiHKCI CYCGRG 1 cut(s) 368
BsiSI CCGG 4 cut(s) 386, 494, 538, 680
BslI CCNNNNNNNGG 7 cut(s) 410, 425, 426, 544, 779, 899, 1035
BsmAI GTCTC 4 cut(s) 80, 229, 464, 476
BsmI GAATGC 2 cut(s) 998, 1145
Bso31I GGTCTC 1 cut(s) 229
BsoBI CYCGRG 1 cut(s) 368
Bsp1286I GDGCHC 3 cut(s) 101, 356, 842
Bsp143I GATC 7 cut(s) 11, 172, 456, 639, 805, 1003, 1127
Bsp1720I GCTNAGC 1 cut(s) 1173
BspACI CCGC 5 cut(s) 68, 342, 500, 754, 1143
BspCNI CTCAG 3 cut(s) 132, 258, 686
BspLI GGNNCC 4 cut(s) 355, 498, 630, 1279
BspT107I GGYRCC 3 cut(s) 496, 628, 1277
BspTNI GGTCTC 1 cut(s) 229
BsrBI CCGCTC 1 cut(s) 502
BsrDI GCAATG 1 cut(s) 616
BsrFI RCCGGY 1 cut(s) 493
BsrI ACTGG 2 cut(s) 430, 1092
BssAI RCCGGY 1 cut(s) 493
BssECI CCNNGG 3 cut(s) 536, 778, 1048
BssMI GATC 7 cut(s) 11, 172, 456, 639, 805, 1003, 1127
BssSI CACGAG 1 cut(s) 408
BssT1I CCWWGG 2 cut(s) 778, 1048
Bst2BI CACGAG 1 cut(s) 408
Bst2UI CCWGG 1 cut(s) 146
Bst4CI ACNGT 3 cut(s) 261, 464, 962
Bst6I CTCTTC 1 cut(s) 474
BstAPI GCANNNNNTGC 1 cut(s) 874
BstC8I GCNNGC 3 cut(s) 251, 715, 998
BstDEI CTNAG 5 cut(s) 140, 245, 507, 673, 1173
BstF5I GGATG 4 cut(s) 58, 415, 528, 1108
BstHHI GCGC 2 cut(s) 255, 380
BstKTI GATC 7 cut(s) 14, 175, 459, 642, 808, 1006, 1130
BstMAI GTCTC 4 cut(s) 80, 229, 464, 476
BstMBI GATC 7 cut(s) 11, 172, 456, 639, 805, 1003, 1127
BstMWI GCNNNNNNNGC 5 cut(s) 141, 351, 499, 874, 1259
BstNI CCWGG 1 cut(s) 146
BstNSI RCATGY 1 cut(s) 846
BstPAI GACNNNNGTC 2 cut(s) 318, 960
BstSCI CCNGG 4 cut(s) 144, 385, 536, 678
BstSFI CTRYAG 1 cut(s) 958
BstSLI GKGCMC 1 cut(s) 842
BstV1I GCAGC 2 cut(s) 100, 228
BstV2I GAAGAC 2 cut(s) 66, 314
BstX2I RGATCY 2 cut(s) 456, 1127
BstXI CCANNNNNNTGG 1 cut(s) 1099
BstYI RGATCY 2 cut(s) 456, 1127
BtgZI GCGATG 1 cut(s) 161
BtrI CACGTC 1 cut(s) 1249
BtsCI GGATG 4 cut(s) 58, 415, 528, 1108
BtsIMutI CAGTG 3 cut(s) 101, 921, 1099
Cac8I GCNNGC 3 cut(s) 251, 715, 998
CfoI GCGC 2 cut(s) 255, 380
Cfr10I RCCGGY 1 cut(s) 493
Csp6I GTAC 4 cut(s) 187, 576, 1043, 1203
CviAII CATG 1 cut(s) 843
CviQI GTAC 4 cut(s) 187, 576, 1043, 1203
DdeI CTNAG 5 cut(s) 140, 245, 507, 673, 1173
DpnI GATC 7 cut(s) 13, 174, 458, 641, 807, 1005, 1129
DpnII GATC 7 cut(s) 11, 172, 456, 639, 805, 1003, 1127
Eam1104I CTCTTC 1 cut(s) 474
EarI CTCTTC 1 cut(s) 474
EciI GGCGGA 1 cut(s) 83
Eco130I CCWWGG 2 cut(s) 778, 1048
Eco24I GRGCYC 1 cut(s) 356
Eco31I GGTCTC 1 cut(s) 229
Eco57I CTGAAG 1 cut(s) 922
Eco88I CYCGRG 1 cut(s) 368
EcoRII CCWGG 1 cut(s) 144
EcoT14I CCWWGG 2 cut(s) 778, 1048
EcoT38I GRGCYC 1 cut(s) 356
ErhI CCWWGG 2 cut(s) 778, 1048
FaeI CATG 1 cut(s) 846
FalI AAGNNNNNCTT 2 cut(s) 143, 175
FatI CATG 1 cut(s) 842
FauNDI CATATG 1 cut(s) 931
FbaI TGATCA 1 cut(s) 805
Fnu4HI GCNGC 5 cut(s) 114, 217, 343, 500, 1143
FokI GGATG 4 cut(s) 65, 402, 515, 1115
FriOI GRGCYC 1 cut(s) 356
Fsp4HI GCNGC 5 cut(s) 114, 217, 343, 500, 1143
FspBI CTAG 2 cut(s) 303, 1049
GlaI GCGC 2 cut(s) 254, 379
GluI GCNGC 5 cut(s) 114, 217, 343, 500, 1143
GsuI CTGGAG 1 cut(s) 805
HapII CCGG 4 cut(s) 386, 494, 538, 680
HhaI GCGC 2 cut(s) 255, 380
Hin1II CATG 1 cut(s) 846
Hin6I GCGC 2 cut(s) 253, 378
HinP1I GCGC 2 cut(s) 253, 378
HindIII AAGCTT 1 cut(s) 748
HinfI GANTC 1 cut(s) 484
HpaII CCGG 4 cut(s) 386, 494, 538, 680
HphI GGTGA 3 cut(s) 212, 434, 1025
Hpy166II GTNNAC 2 cut(s) 553, 1045
Hpy188III TCNNGA 1 cut(s) 167
Hpy8I GTNNAC 2 cut(s) 553, 1045
Hpy99I CGWCG 2 cut(s) 173, 317
HpyAV CCTTC 4 cut(s) 169, 987, 1079, 1291
HpyCH4III ACNGT 3 cut(s) 261, 464, 962
HpyCH4IV ACGT 2 cut(s) 567, 1248
HpyCH4V TGCA 6 cut(s) 116, 707, 713, 919, 970, 996
HpyF10VI GCNNNNNNNGC 5 cut(s) 141, 351, 499, 874, 1259
HpyF3I CTNAG 5 cut(s) 140, 245, 507, 673, 1173
HpySE526I ACGT 2 cut(s) 567, 1248
Hsp92II CATG 1 cut(s) 846
HspAI GCGC 2 cut(s) 253, 378
Ksp22I TGATCA 1 cut(s) 805
Kzo9I GATC 7 cut(s) 11, 172, 456, 639, 805, 1003, 1127
LmnI GCTCC 2 cut(s) 359, 507
Lsp1109I GCAGC 2 cut(s) 100, 228
LweI GCATC 7 cut(s) 424, 448, 504, 928, 954, 979, 1260
MaeI CTAG 2 cut(s) 303, 1049
MaeII ACGT 2 cut(s) 567, 1248
MaeIII GTNAC 3 cut(s) 200, 298, 686
MalI GATC 7 cut(s) 13, 174, 458, 641, 807, 1005, 1129
MbiI CCGCTC 1 cut(s) 502
MboI GATC 7 cut(s) 11, 172, 456, 639, 805, 1003, 1127
MflI RGATCY 2 cut(s) 456, 1127
MhlI GDGCHC 3 cut(s) 101, 356, 842
MlyI GAGTC 1 cut(s) 478
MmeI TCCRAC 3 cut(s) 336, 428, 913
MnlI CCTC 8 cut(s) 200, 336, 404, 541, 645, 904, 1101, 1209
MroXI GAANNNNTTC 1 cut(s) 158
MseI TTAA 3 cut(s) 26, 834, 986
MslI CAYNNNNRTG 1 cut(s) 914
MspA1I CMGCKG 2 cut(s) 295, 767
MspI CCGG 4 cut(s) 386, 494, 538, 680
MspR9I CCNGG 4 cut(s) 146, 387, 538, 680
Mva1269I GAATGC 2 cut(s) 998, 1145
MvaI CCWGG 1 cut(s) 146
MwoI GCNNNNNNNGC 5 cut(s) 141, 351, 499, 874, 1259
NciI CCSGG 3 cut(s) 387, 538, 680
NdeI CATATG 1 cut(s) 931
NdeII GATC 7 cut(s) 11, 172, 456, 639, 805, 1003, 1127
NlaIII CATG 1 cut(s) 846
NlaIV GGNNCC 4 cut(s) 355, 498, 630, 1279
NmuCI GTSAC 2 cut(s) 200, 686
NspI RCATGY 1 cut(s) 846
PciI ACATGT 1 cut(s) 842
PcsI WCGNNNNNNNCGW 2 cut(s) 11, 204
PctI GAATGC 2 cut(s) 998, 1145
PdmI GAANNNNTTC 1 cut(s) 158
PkrI GCNGC 5 cut(s) 115, 218, 344, 501, 1144
PleI GAGTC 1 cut(s) 478
PpsI GAGTC 1 cut(s) 478
PscI ACATGT 1 cut(s) 842
PshAI GACNNNNGTC 2 cut(s) 318, 960
Psp6I CCWGG 1 cut(s) 144
PspGI CCWGG 1 cut(s) 144
PspN4I GGNNCC 4 cut(s) 355, 498, 630, 1279
PsuI RGATCY 2 cut(s) 456, 1127
PvuII CAGCTG 2 cut(s) 295, 767
RsaI GTAC 4 cut(s) 188, 577, 1044, 1204
RsaNI GTAC 4 cut(s) 187, 576, 1043, 1203
RseI CAYNNNNRTG 1 cut(s) 914
SaqAI TTAA 3 cut(s) 26, 834, 986
SatI GCNGC 5 cut(s) 114, 217, 343, 500, 1143
Sau3AI GATC 7 cut(s) 11, 172, 456, 639, 805, 1003, 1127
SchI GAGTC 1 cut(s) 478
ScrFI CCNGG 4 cut(s) 146, 387, 538, 680
SduI GDGCHC 3 cut(s) 101, 356, 842
SfaNI GCATC 7 cut(s) 424, 448, 504, 928, 954, 979, 1260
SfcI CTRYAG 1 cut(s) 958
SmiMI CAYNNNNRTG 1 cut(s) 914
SmlI CTYRAG 1 cut(s) 486
SmoI CTYRAG 1 cut(s) 486
SsiI CCGC 5 cut(s) 68, 342, 500, 754, 1143
SspMI CTAG 2 cut(s) 303, 1049
StyD4I CCNGG 4 cut(s) 144, 385, 536, 678
StyI CCWWGG 2 cut(s) 778, 1048
TaaI ACNGT 3 cut(s) 261, 464, 962
TaiI ACGT 2 cut(s) 570, 1251
TaqI TCGA 2 cut(s) 14, 693
TauI GCSGC 3 cut(s) 345, 502, 1145
Tru1I TTAA 3 cut(s) 26, 834, 986
Tru9I TTAA 3 cut(s) 26, 834, 986
TscAI CASTG 3 cut(s) 101, 921, 1099
TseFI GTSAC 2 cut(s) 200, 686
TseI GCWGC 2 cut(s) 113, 216
Tsp45I GTSAC 2 cut(s) 200, 686
TspDTI ATGAA 5 cut(s) 69, 151, 261, 918, 1011
TspRI CASTG 3 cut(s) 101, 921, 1099
XapI RAATTY 3 cut(s) 17, 360, 1136
XceI RCATGY 1 cut(s) 846
XcmI CCANNNNNNNNNTGG 2 cut(s) 896, 1036
XmaJI CCTAGG 1 cut(s) 1048
XmnI GAANNNNTTC 1 cut(s) 158
XspI CTAG 2 cut(s) 303, 1049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.