RchiOBHm_Chr5g0046531

Transferase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0004543
Physical Location & Seq
Reverse (-)
42587000 .. 42587476
477 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ32452

Sequence Viewer

Length: 477 bp
ATGATGAAGCAGAACAAGAGCAACCGGGTGGCTGTCCGTTCAAAGCTAACGGCAGTATCCAGCCGGCCGGTGAACCCGGGTACGATCCATTCTTTCACGGGTCTGGATCATGCAATGGCCCTGCACAGCGTTCACGTGATATTCTACTACAAGGAGAAGCTGTTTTCATATTTTGACTTGGACCCGTTTAGGGTGTGTCTCTCGGAGACCCTATCGCTGTACCCGCCAGTGACGGGTAGGATAGTCCGGAGAGAGGTCGACGGTGAGTGGGAGGTCAAGTGTAACGATGCCGGCGTGAGGGTGGTGAAGGCCACGGTGGCGACAACACTTGATGAGTGGCTGGCAACTGCTGATGGGGCGGAGGAGAGGTTGTTGACGGCGTGGGATGATATGCCTCATGGTGATTCTAGTTCATGGTCGCCGTATAGAATCCAGGTGCGTACTCCGATCCGGCCGGCTCTTTCCCTCTCTTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transferase

Protein Analysis

158

Amino Acids

17.83

Weight (kDa)

7.82

Isoelectric Point (pI)

39.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Transferase PF02458 33 - 129 9.8e-14 Transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 258
AccIII TCCGGA 1 cut(s) 246
AciI CCGC 2 cut(s) 224, 359
AclWI GGATC 3 cut(s) 79, 114, 442
AcoI YGGCCR 2 cut(s) 65, 452
AcvI CACGTG 1 cut(s) 136
AfaI GTAC 3 cut(s) 82, 221, 442
AfiI CCNNNNNNNGG 4 cut(s) 190, 233, 253, 297
AgsI TTSAA 1 cut(s) 42
AjnI CCWGG 1 cut(s) 432
AluBI AGCT 2 cut(s) 46, 160
AluI AGCT 2 cut(s) 46, 160
Alw26I GTCTC 2 cut(s) 200, 203
AlwI GGATC 3 cut(s) 79, 114, 442
Ama87I CYCGRG 1 cut(s) 76
Aor13HI TCCGGA 1 cut(s) 246
AoxI GGCC 4 cut(s) 65, 117, 309, 452
AspS9I GGNCC 2 cut(s) 118, 181
AsuC2I CCSGG 3 cut(s) 26, 77, 78
AsuHPI GGTGA 4 cut(s) 82, 275, 316, 413
AvaI CYCGRG 1 cut(s) 76
AvaII GGWCC 1 cut(s) 181
BaeI ACNNNNGTAYC 2 cut(s) 39, 72
BbrPI CACGTG 1 cut(s) 136
BccI CCATC 1 cut(s) 347
BceAI ACGGC 3 cut(s) 66, 393, 406
BciT130I CCWGG 1 cut(s) 434
BciVI GTATCC 1 cut(s) 67
BcnI CCSGG 3 cut(s) 26, 77, 78
BcoDI GTCTC 2 cut(s) 200, 203
BfaI CTAG 1 cut(s) 408
BfuI GTATCC 1 cut(s) 67
BglI GCCNNNNNGGC 1 cut(s) 317
Bme1390I CCNGG 4 cut(s) 26, 77, 78, 434
Bme18I GGWCC 1 cut(s) 181
BmeT110I CYCGRG 1 cut(s) 76
BmgT120I GGNCC 2 cut(s) 118, 181
BmiI GGNNCC 1 cut(s) 183
BmrFI CCNGG 4 cut(s) 26, 77, 78, 434
BmsI GCATC 1 cut(s) 277
BpuMI CCSGG 3 cut(s) 26, 77, 78
BsaAI YACGTR 1 cut(s) 136
BsaI GGTCTC 1 cut(s) 200
BsaJI CCNNGG 2 cut(s) 76, 312
BsaWI WCCGGW 1 cut(s) 246
BsaXI ACNNNNNCTCC 8 cut(s) 146, 176, 263, 293, 353, 356, 383, 386
Bsc4I CCNNNNNNNGG 4 cut(s) 190, 233, 253, 297
Bse118I RCCGGY 4 cut(s) 63, 67, 290, 454
Bse1I ACTGG 1 cut(s) 227
Bse3DI GCAATG 1 cut(s) 120
BseAI TCCGGA 1 cut(s) 246
BseBI CCWGG 1 cut(s) 434
BseDI CCNNGG 2 cut(s) 76, 312
BseGI GGATG 1 cut(s) 391
BseLI CCNNNNNNNGG 4 cut(s) 190, 233, 253, 297
BseMI GCAATG 1 cut(s) 120
BseNI ACTGG 1 cut(s) 227
BseRI GAGGAG 1 cut(s) 377
BseX3I CGGCCG 2 cut(s) 65, 452
BsgI GTGCAG 1 cut(s) 107
Bsh1285I CGRYCG 2 cut(s) 68, 455
BshFI GGCC 4 cut(s) 67, 119, 311, 454
BsiEI CGRYCG 2 cut(s) 68, 455
BsiHKCI CYCGRG 1 cut(s) 76
BsiSI CCGG 8 cut(s) 25, 64, 68, 77, 247, 291, 451, 455
BslI CCNNNNNNNGG 4 cut(s) 190, 233, 253, 297
BsmAI GTCTC 2 cut(s) 200, 203
BsnI GGCC 4 cut(s) 67, 119, 311, 454
Bso31I GGTCTC 1 cut(s) 200
BsoBI CYCGRG 1 cut(s) 76
Bsp13I TCCGGA 1 cut(s) 246
Bsp143I GATC 3 cut(s) 84, 106, 447
BspACI CCGC 2 cut(s) 224, 359
BspANI GGCC 4 cut(s) 67, 119, 311, 454
BspEI TCCGGA 1 cut(s) 246
BspLI GGNNCC 1 cut(s) 183
BspPI GGATC 3 cut(s) 79, 114, 442
BspTNI GGTCTC 1 cut(s) 200
BsrDI GCAATG 1 cut(s) 120
BsrFI RCCGGY 4 cut(s) 63, 67, 290, 454
BsrI ACTGG 1 cut(s) 227
BssAI RCCGGY 4 cut(s) 63, 67, 290, 454
BssECI CCNNGG 2 cut(s) 76, 312
BssMI GATC 3 cut(s) 84, 106, 447
Bst2UI CCWGG 1 cut(s) 434
Bst4CI ACNGT 2 cut(s) 263, 316
BstBAI YACGTR 1 cut(s) 136
BstC8I GCNNGC 4 cut(s) 65, 292, 342, 456
BstDSI CCRYGG 1 cut(s) 312
BstF5I GGATG 1 cut(s) 391
BstKTI GATC 3 cut(s) 87, 109, 450
BstMAI GTCTC 2 cut(s) 200, 203
BstMBI GATC 3 cut(s) 84, 106, 447
BstMCI CGRYCG 2 cut(s) 68, 455
BstMWI GCNNNNNNNGC 3 cut(s) 223, 317, 356
BstNI CCWGG 1 cut(s) 434
BstSCI CCNGG 4 cut(s) 24, 75, 76, 432
BstZI CGGCCG 2 cut(s) 65, 452
BsuI GTATCC 1 cut(s) 67
BsuRI GGCC 4 cut(s) 67, 119, 311, 454
BtgI CCRYGG 1 cut(s) 312
BtsCI GGATG 1 cut(s) 391
BtsIMutI CAGTG 1 cut(s) 234
Cac8I GCNNGC 4 cut(s) 65, 292, 342, 456
Cfr10I RCCGGY 4 cut(s) 63, 67, 290, 454
Cfr13I GGNCC 2 cut(s) 118, 181
Cfr9I CCCGGG 1 cut(s) 76
Csp6I GTAC 3 cut(s) 81, 220, 441
CviAII CATG 3 cut(s) 110, 398, 414
CviQI GTAC 3 cut(s) 81, 220, 441
DpnI GATC 3 cut(s) 86, 108, 449
DpnII GATC 3 cut(s) 84, 106, 447
EaeI YGGCCR 2 cut(s) 65, 452
EagI CGGCCG 2 cut(s) 65, 452
EciI GGCGGA 1 cut(s) 374
EclXI CGGCCG 2 cut(s) 65, 452
Eco31I GGTCTC 1 cut(s) 200
Eco47I GGWCC 1 cut(s) 181
Eco52I CGGCCG 2 cut(s) 65, 452
Eco72I CACGTG 1 cut(s) 136
Eco88I CYCGRG 1 cut(s) 76
EcoRII CCWGG 1 cut(s) 432
FaeI CATG 3 cut(s) 113, 401, 417
FaiI YATR 6 cut(s) 111, 169, 392, 399, 415, 426
FatI CATG 3 cut(s) 109, 397, 413
FauI CCCGC 1 cut(s) 231
FblI GTMKAC 1 cut(s) 258
FokI GGATG 1 cut(s) 398
FspBI CTAG 1 cut(s) 408
HaeIII GGCC 4 cut(s) 67, 119, 311, 454
HapII CCGG 8 cut(s) 25, 64, 68, 77, 247, 291, 451, 455
Hin1II CATG 3 cut(s) 113, 401, 417
HincII GTYRAC 2 cut(s) 259, 375
HindII GTYRAC 2 cut(s) 259, 375
HinfI GANTC 2 cut(s) 404, 429
HpaII CCGG 8 cut(s) 25, 64, 68, 77, 247, 291, 451, 455
HphI GGTGA 4 cut(s) 82, 275, 316, 413
Hpy166II GTNNAC 4 cut(s) 73, 133, 259, 375
Hpy188I TCNGA 2 cut(s) 205, 447
Hpy188III TCNNGA 2 cut(s) 104, 247
Hpy8I GTNNAC 4 cut(s) 73, 133, 259, 375
Hpy99I CGWCG 1 cut(s) 263
HpyAV CCTTC 1 cut(s) 301
HpyCH4III ACNGT 2 cut(s) 263, 316
HpyCH4IV ACGT 1 cut(s) 135
HpyCH4V TGCA 2 cut(s) 113, 124
HpyF10VI GCNNNNNNNGC 3 cut(s) 223, 317, 356
HpySE526I ACGT 1 cut(s) 135
Hsp92II CATG 3 cut(s) 113, 401, 417
Kpn2I TCCGGA 1 cut(s) 246
KroI GCCGGC 3 cut(s) 63, 290, 454
KroNI GCCGGC 3 cut(s) 65, 292, 456
Kzo9I GATC 3 cut(s) 84, 106, 447
LweI GCATC 1 cut(s) 277
MaeI CTAG 1 cut(s) 408
MaeII ACGT 1 cut(s) 135
MaeIII GTNAC 2 cut(s) 229, 281
MalI GATC 3 cut(s) 86, 108, 449
MboI GATC 3 cut(s) 84, 106, 447
MnlI CCTC 7 cut(s) 247, 265, 291, 355, 360, 405, 476
MroI TCCGGA 1 cut(s) 246
MroNI GCCGGC 3 cut(s) 63, 290, 454
MseI TTAA 1 cut(s) 475
MspI CCGG 8 cut(s) 25, 64, 68, 77, 247, 291, 451, 455
MspR9I CCNGG 4 cut(s) 26, 77, 78, 434
MvaI CCWGG 1 cut(s) 434
MwoI GCNNNNNNNGC 3 cut(s) 223, 317, 356
NaeI GCCGGC 3 cut(s) 65, 292, 456
NciI CCSGG 3 cut(s) 26, 77, 78
NdeII GATC 3 cut(s) 84, 106, 447
NgoMIV GCCGGC 3 cut(s) 63, 290, 454
NlaIII CATG 3 cut(s) 113, 401, 417
NlaIV GGNNCC 1 cut(s) 183
NmuCI GTSAC 1 cut(s) 229
PcsI WCGNNNNNNNCGW 1 cut(s) 291
PdiI GCCGGC 3 cut(s) 65, 292, 456
PfeI GAWTC 2 cut(s) 404, 429
PmaCI CACGTG 1 cut(s) 136
PmlI CACGTG 1 cut(s) 136
Ppu21I YACGTR 1 cut(s) 136
Psp6I CCWGG 1 cut(s) 432
PspCI CACGTG 1 cut(s) 136
PspGI CCWGG 1 cut(s) 432
PspN4I GGNNCC 1 cut(s) 183
PspPI GGNCC 2 cut(s) 118, 181
RsaI GTAC 3 cut(s) 82, 221, 442
RsaNI GTAC 3 cut(s) 81, 220, 441
SalI GTCGAC 1 cut(s) 257
SaqAI TTAA 1 cut(s) 475
Sau3AI GATC 3 cut(s) 84, 106, 447
Sau96I GGNCC 2 cut(s) 118, 181
ScrFI CCNGG 4 cut(s) 26, 77, 78, 434
SetI ASST 7 cut(s) 48, 138, 162, 258, 276, 371, 438
SfaNI GCATC 1 cut(s) 277
SinI GGWCC 1 cut(s) 181
SmaI CCCGGG 1 cut(s) 78
SsiI CCGC 2 cut(s) 224, 359
SspMI CTAG 1 cut(s) 408
StyD4I CCNGG 4 cut(s) 24, 75, 76, 432
TaaI ACNGT 2 cut(s) 263, 316
TaiI ACGT 1 cut(s) 138
TaqI TCGA 1 cut(s) 258
TfiI GAWTC 2 cut(s) 404, 429
Tru1I TTAA 1 cut(s) 475
Tru9I TTAA 1 cut(s) 475
TscAI CASTG 1 cut(s) 234
TseFI GTSAC 1 cut(s) 229
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 3 cut(s) 20, 156, 402
TspGWI ACGGA 1 cut(s) 26
TspMI CCCGGG 1 cut(s) 76
TspRI CASTG 1 cut(s) 234
VpaK11BI GGWCC 1 cut(s) 181
XmaI CCCGGG 1 cut(s) 76
XmiI GTMKAC 1 cut(s) 258
XspI CTAG 1 cut(s) 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.