Rroxscaffold_3G00232570

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004543
Physical Location & Seq
Forward (+)
18207817 .. 18208628
812 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00232570.1

Sequence Viewer

Length: 258 bp
ATGGAGTCGACGAGGATTACGATTCATGGTCCTTCCTCCGTGGCCACTAGTCGAGCCGCGAAACAAAAAGTCGTGGCGGAGGAACCTAAGGTTGAAGATTCTTCGGGTGATGAAGATCCCGTAGAGGTTTGTCAAAGGACCACCCAGTCCCTCCAGGCAAAGAGGGTAGCCCATCCACAACTAGAGGGCAAGCGGCCTCTTCGCGGGACCAAATGCCCTCGAAGCCCGGTGACCCCTAATCTTGACCTTGCCTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

85

Amino Acids

9.22

Weight (kDa)

8.74

Isoelectric Point (pI)

67.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 145
AccI GTMKAC 1 cut(s) 8
AccII CGCG 2 cut(s) 59, 204
AciI CCGC 4 cut(s) 57, 77, 193, 204
AclWI GGATC 1 cut(s) 110
AcoI YGGCCR 1 cut(s) 42
AfiI CCNNNNNNNGG 1 cut(s) 203
AgsI TTSAA 1 cut(s) 95
AhlI ACTAGT 1 cut(s) 47
AjnI CCWGG 1 cut(s) 153
AlwI GGATC 1 cut(s) 110
AoxI GGCC 2 cut(s) 42, 194
AspS9I GGNCC 3 cut(s) 29, 138, 207
AsuC2I CCSGG 1 cut(s) 227
AsuHPI GGTGA 2 cut(s) 119, 241
AvaII GGWCC 3 cut(s) 29, 138, 207
AxyI CCTNAGG 1 cut(s) 87
BalI TGGCCA 1 cut(s) 44
BccI CCATC 1 cut(s) 180
BciT130I CCWGG 1 cut(s) 155
BcnI CCSGG 1 cut(s) 227
BcuI ACTAGT 1 cut(s) 47
BfaI CTAG 2 cut(s) 48, 182
BisI GCNGC 2 cut(s) 57, 194
BlsI GCNGC 2 cut(s) 58, 195
Bme1390I CCNGG 2 cut(s) 155, 227
Bme18I GGWCC 3 cut(s) 29, 138, 207
BmgT120I GGNCC 3 cut(s) 29, 138, 207
BmiI GGNNCC 2 cut(s) 84, 208
BmrFI CCNGG 2 cut(s) 155, 227
BmrI ACTGGG 1 cut(s) 139
BmuI ACTGGG 1 cut(s) 139
BpmI CTGGAG 1 cut(s) 137
BpuMI CCSGG 1 cut(s) 227
BsaBI GATNNNNATC 1 cut(s) 114
BsaJI CCNNGG 1 cut(s) 39
Bsc4I CCNNNNNNNGG 1 cut(s) 203
Bse1I ACTGG 1 cut(s) 145
Bse21I CCTNAGG 1 cut(s) 87
Bse8I GATNNNNATC 1 cut(s) 114
BseBI CCWGG 1 cut(s) 155
BseDI CCNNGG 1 cut(s) 39
BseGI GGATG 1 cut(s) 172
BseJI GATNNNNATC 1 cut(s) 114
BseLI CCNNNNNNNGG 1 cut(s) 203
BseNI ACTGG 1 cut(s) 145
Bsh1236I CGCG 2 cut(s) 59, 204
BshFI GGCC 2 cut(s) 44, 196
BsiSI CCGG 1 cut(s) 227
BslFI GGGAC 2 cut(s) 133, 220
BslI CCNNNNNNNGG 1 cut(s) 203
BsmFI GGGAC 2 cut(s) 133, 220
BsnI GGCC 2 cut(s) 44, 196
Bsp143I GATC 1 cut(s) 115
BspACI CCGC 4 cut(s) 57, 77, 193, 204
BspANI GGCC 2 cut(s) 44, 196
BspFNI CGCG 2 cut(s) 59, 204
BspLI GGNNCC 2 cut(s) 84, 208
BspPI GGATC 1 cut(s) 110
BsrI ACTGG 1 cut(s) 145
BssECI CCNNGG 1 cut(s) 39
BssMI GATC 1 cut(s) 115
Bst2UI CCWGG 1 cut(s) 155
Bst6I CTCTTC 1 cut(s) 204
BstC8I GCNNGC 1 cut(s) 191
BstDEI CTNAG 1 cut(s) 87
BstDSI CCRYGG 1 cut(s) 39
BstEII GGTNACC 1 cut(s) 229
BstF5I GGATG 1 cut(s) 172
BstFNI CGCG 2 cut(s) 59, 204
BstKTI GATC 1 cut(s) 118
BstMBI GATC 1 cut(s) 115
BstMWI GCNNNNNNNGC 1 cut(s) 222
BstNI CCWGG 1 cut(s) 155
BstPI GGTNACC 1 cut(s) 229
BstSCI CCNGG 2 cut(s) 153, 225
BstUI CGCG 2 cut(s) 59, 204
BstX2I RGATCY 1 cut(s) 115
BstYI RGATCY 1 cut(s) 115
Bsu36I CCTNAGG 1 cut(s) 87
BsuRI GGCC 2 cut(s) 44, 196
BtgI CCRYGG 1 cut(s) 39
BtsCI GGATG 1 cut(s) 172
Cac8I GCNNGC 1 cut(s) 191
Cfr13I GGNCC 3 cut(s) 29, 138, 207
CviAII CATG 1 cut(s) 26
CviJI RGCY 5 cut(s) 44, 56, 170, 196, 225
CviKI_1 RGCY 5 cut(s) 44, 56, 170, 196, 225
DdeI CTNAG 1 cut(s) 87
DpnI GATC 1 cut(s) 117
DpnII GATC 1 cut(s) 115
DrdI GACNNNNNNGTC 1 cut(s) 145
DseDI GACNNNNNNGTC 1 cut(s) 145
EaeI YGGCCR 1 cut(s) 42
Eam1104I CTCTTC 1 cut(s) 204
EarI CTCTTC 1 cut(s) 204
EciI GGCGGA 1 cut(s) 92
Eco47I GGWCC 3 cut(s) 29, 138, 207
Eco81I CCTNAGG 1 cut(s) 87
Eco91I GGTNACC 1 cut(s) 229
EcoO65I GGTNACC 1 cut(s) 229
EcoRII CCWGG 1 cut(s) 153
FaeI CATG 1 cut(s) 29
FaiI YATR 1 cut(s) 27
FaqI GGGAC 2 cut(s) 133, 220
FatI CATG 1 cut(s) 25
FauI CCCGC 1 cut(s) 197
FblI GTMKAC 1 cut(s) 8
Fnu4HI GCNGC 2 cut(s) 57, 194
FokI GGATG 1 cut(s) 159
Fsp4HI GCNGC 2 cut(s) 57, 194
FspBI CTAG 2 cut(s) 48, 182
GluI GCNGC 2 cut(s) 57, 194
GsuI CTGGAG 1 cut(s) 137
HaeIII GGCC 2 cut(s) 44, 196
HapII CCGG 1 cut(s) 227
Hin1II CATG 1 cut(s) 29
HincII GTYRAC 1 cut(s) 9
HindII GTYRAC 1 cut(s) 9
HinfI GANTC 3 cut(s) 5, 22, 98
HpaII CCGG 1 cut(s) 227
HphI GGTGA 2 cut(s) 119, 241
Hpy166II GTNNAC 1 cut(s) 9
Hpy188III TCNNGA 1 cut(s) 242
Hpy8I GTNNAC 1 cut(s) 9
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 1 cut(s) 42
HpyF10VI GCNNNNNNNGC 1 cut(s) 222
HpyF3I CTNAG 1 cut(s) 87
Hsp92II CATG 1 cut(s) 29
Kzo9I GATC 1 cut(s) 115
LpnPI CCDG 4 cut(s) 140, 158, 167, 240
MaeI CTAG 2 cut(s) 48, 182
MaeIII GTNAC 1 cut(s) 229
MalI GATC 1 cut(s) 117
MboI GATC 1 cut(s) 115
MboII GAAGA 4 cut(s) 93, 107, 125, 191
MflI RGATCY 1 cut(s) 115
MlsI TGGCCA 1 cut(s) 44
MluNI TGGCCA 1 cut(s) 44
MlyI GAGTC 1 cut(s) 14
MnlI CCTC 9 cut(s) 6, 46, 73, 118, 156, 161, 178, 207, 228
Mox20I TGGCCA 1 cut(s) 44
MscI TGGCCA 1 cut(s) 44
Msp20I TGGCCA 1 cut(s) 44
MspI CCGG 1 cut(s) 227
MspR9I CCNGG 2 cut(s) 155, 227
MvaI CCWGG 1 cut(s) 155
MvnI CGCG 2 cut(s) 59, 204
MwoI GCNNNNNNNGC 1 cut(s) 222
NciI CCSGG 1 cut(s) 227
NdeII GATC 1 cut(s) 115
NlaIII CATG 1 cut(s) 29
NlaIV GGNNCC 2 cut(s) 84, 208
NmuCI GTSAC 1 cut(s) 229
PcsI WCGNNNNNNNCGW 1 cut(s) 17
PfeI GAWTC 2 cut(s) 22, 98
PkrI GCNGC 2 cut(s) 58, 195
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Psp6I CCWGG 1 cut(s) 153
PspEI GGTNACC 1 cut(s) 229
PspGI CCWGG 1 cut(s) 153
PspN4I GGNNCC 2 cut(s) 84, 208
PspPI GGNCC 3 cut(s) 29, 138, 207
PsuI RGATCY 1 cut(s) 115
SalI GTCGAC 1 cut(s) 7
SatI GCNGC 2 cut(s) 57, 194
Sau3AI GATC 1 cut(s) 115
Sau96I GGNCC 3 cut(s) 29, 138, 207
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 2 cut(s) 155, 227
SetI ASST 4 cut(s) 88, 93, 129, 249
SinI GGWCC 3 cut(s) 29, 138, 207
SpeI ACTAGT 1 cut(s) 47
SsiI CCGC 4 cut(s) 57, 77, 193, 204
SspMI CTAG 2 cut(s) 48, 182
StyD4I CCNGG 2 cut(s) 153, 225
TaqI TCGA 3 cut(s) 8, 52, 220
TauI GCSGC 2 cut(s) 59, 196
TfiI GAWTC 2 cut(s) 22, 98
TseFI GTSAC 1 cut(s) 229
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 2 cut(s) 14, 126
TspGWI ACGGA 1 cut(s) 28
VpaK11BI GGWCC 3 cut(s) 29, 138, 207
XmiI GTMKAC 1 cut(s) 8
XspI CTAG 2 cut(s) 48, 182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.