RchiOBHm_Chr5g0062181

Zinc finger protein CONSTANS-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003558
Physical Location & Seq
Forward (+)
67718867 .. 67721583
2717 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33848

Sequence Viewer

Length: 885 bp
ATGGCCCCCCGCCCTTTTGCTTTAAGTACCCCCCATCCCTCCTCCAGTATATTCACTCCTCTCAAACCTTCCCACAACTCCCAAAAATTCTCGACCGACTTCCCCACACGCGCCATGTACGGTCACAACGGCAACGCATTCCCACGCGCCAACGACTTCGAGGGCCTGCCAATGCCGGCCCTGCCCTTCGGGACATACAACAATGACTACCACCACCTCGGCGCGGCGGCGAATGAGTTCAGCAACAACAGCTGTAGCAGTGGCTGCAGCAGCTACGGTGGCTCGCCCACCTATGCGAGTACCACTACCACTACGACTATTCATGATCATGAGGTTGAGCCGAGCCTGATGATTCAGAGGAGTGTGAGCAGCCACTCGCTCCAGAAGAACATCGGGACCCACCGGCTCGTCTCGGACTTGCTGGAGCTCGAGACCGGCCCCGTCCGGAGGGTTTATAGCACCGGCGACTTGGATTTGCAGAGGATTAACAATACTAATGGGGTGCAAGTGCAGCAATTGCATAGCAGCTACAGATCATCATCAGAAAGTAGTCCATTGTCAAGCGAGAGCAGTATGATCATTGAAGGAATGAGCAAAGCCTGTCGTTACAGTCCTGAGGAGAAGAAAGAGAGAATCGAGAGGTACAGAAGCAAGAGAAACCTCAGGAACTTCAACAAAACGATTAAGTATGCATGTAGGAAGACATTGGCGGACAGCAGGCCGCGCATCAGAGGGCGATTTGCAAGGAATGATGAAATTGATCAGAAGAATTCCCCTAATGTTCAGTGGAGCCAAATGAGTGGGGAGGAAGATGAAGAGGATGGCGATGGTTGGATCAACCTGCTTGATGCATTCTCTTCAATCGAATCAAATTCCTTAATCTAG

Protein Analysis

294

Amino Acids

32.85

Weight (kDa)

6.8

Isoelectric Point (pI)

69.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCT PF06203 209 - 250 1.6e-18 CCT motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 849
AccII CGCG 4 cut(s) 111, 147, 224, 724
AccIII TCCGGA 1 cut(s) 444
AciI CCGC 5 cut(s) 10, 224, 227, 710, 722
AclWI GGATC 1 cut(s) 842
AcsI RAATTY 3 cut(s) 86, 769, 871
AfaI GTAC 4 cut(s) 28, 119, 301, 644
AfiI CCNNNNNNNGG 2 cut(s) 223, 447
AgsI TTSAA 3 cut(s) 584, 673, 861
AluBI AGCT 4 cut(s) 252, 273, 427, 528
AluI AGCT 4 cut(s) 252, 273, 427, 528
Alw21I GWGCWC 1 cut(s) 429
Alw26I GTCTC 2 cut(s) 415, 425
AlwI GGATC 1 cut(s) 842
AlwNI CAGNNNCTG 1 cut(s) 264
Ama87I CYCGRG 1 cut(s) 428
Aor13HI TCCGGA 1 cut(s) 444
AoxI GGCC 5 cut(s) 3, 163, 177, 436, 719
ApeKI GCWGC 6 cut(s) 264, 267, 270, 369, 511, 525
ApoI RAATTY 3 cut(s) 86, 769, 871
Asp700I GAANNNNTTC 1 cut(s) 236
AspLEI GCGC 4 cut(s) 113, 149, 224, 726
AspS9I GGNCC 5 cut(s) 4, 163, 178, 396, 437
AvaI CYCGRG 1 cut(s) 428
AvaII GGWCC 1 cut(s) 396
AxyI CCTNAGG 2 cut(s) 615, 662
BanII GRGCYC 1 cut(s) 429
BbsI GAAGAC 1 cut(s) 707
Bbv12I GWGCWC 1 cut(s) 429
BbvI GCAGC 6 cut(s) 251, 279, 282, 381, 523, 537
BccI CCATC 3 cut(s) 42, 815, 821
BceAI ACGGC 1 cut(s) 145
BclI TGATCA 3 cut(s) 325, 576, 760
BcoDI GTCTC 2 cut(s) 415, 425
BfaI CTAG 1 cut(s) 883
BfmI CTRYAG 3 cut(s) 253, 265, 529
BfuAI ACCTGC 1 cut(s) 849
BisI GCNGC 9 cut(s) 225, 228, 265, 268, 271, 370, 512, 526, 722
BlsI GCNGC 9 cut(s) 226, 229, 266, 269, 272, 371, 513, 527, 723
Bme18I GGWCC 1 cut(s) 396
BmeT110I CYCGRG 1 cut(s) 428
BmgT120I GGNCC 5 cut(s) 4, 163, 178, 396, 437
BmiI GGNNCC 5 cut(s) 6, 397, 398, 439, 791
BmsI GCATC 2 cut(s) 735, 838
BpiI GAAGAC 1 cut(s) 707
BpmI CTGGAG 3 cut(s) 28, 365, 443
BsaBI GATNNNNATC 1 cut(s) 538
BsaI GGTCTC 1 cut(s) 425
BsaJI CCNNGG 1 cut(s) 217
BsaWI WCCGGW 1 cut(s) 444
Bsc4I CCNNNNNNNGG 2 cut(s) 223, 447
Bse118I RCCGGY 4 cut(s) 175, 402, 434, 461
Bse1I ACTGG 1 cut(s) 45
Bse21I CCTNAGG 2 cut(s) 615, 662
Bse8I GATNNNNATC 1 cut(s) 538
BseAI TCCGGA 1 cut(s) 444
BseDI CCNNGG 1 cut(s) 217
BseGI GGATG 2 cut(s) 34, 826
BseJI GATNNNNATC 1 cut(s) 538
BseLI CCNNNNNNNGG 2 cut(s) 223, 447
BseMII CTCAG 2 cut(s) 606, 676
BseNI ACTGG 1 cut(s) 45
BseRI GAGGAG 4 cut(s) 31, 48, 373, 632
BseXI GCAGC 6 cut(s) 251, 279, 282, 381, 523, 537
BsgI GTGCAG 1 cut(s) 530
Bsh1236I CGCG 4 cut(s) 111, 147, 224, 724
Bsh1285I CGRYCG 1 cut(s) 96
BshFI GGCC 5 cut(s) 5, 165, 179, 438, 721
BsiEI CGRYCG 1 cut(s) 96
BsiHKAI GWGCWC 1 cut(s) 429
BsiHKCI CYCGRG 1 cut(s) 428
BsiSI CCGG 5 cut(s) 176, 403, 435, 445, 462
BslFI GGGAC 2 cut(s) 205, 409
BslI CCNNNNNNNGG 2 cut(s) 223, 447
BsmAI GTCTC 2 cut(s) 415, 425
BsmBI CGTCTC 1 cut(s) 415
BsmFI GGGAC 2 cut(s) 205, 409
BsmI GAATGC 2 cut(s) 137, 851
BsnI GGCC 5 cut(s) 5, 165, 179, 438, 721
Bso31I GGTCTC 1 cut(s) 425
BsoBI CYCGRG 1 cut(s) 428
Bsp1286I GDGCHC 1 cut(s) 429
Bsp13I TCCGGA 1 cut(s) 444
Bsp143I GATC 5 cut(s) 325, 533, 576, 760, 834
BspACI CCGC 5 cut(s) 10, 224, 227, 710, 722
BspANI GGCC 5 cut(s) 5, 165, 179, 438, 721
BspCNI CTCAG 2 cut(s) 607, 675
BspEI TCCGGA 1 cut(s) 444
BspFNI CGCG 4 cut(s) 111, 147, 224, 724
BspHI TCATGA 2 cut(s) 322, 328
BspLI GGNNCC 5 cut(s) 6, 397, 398, 439, 791
BspMAI CTGCAG 1 cut(s) 269
BspMI ACCTGC 1 cut(s) 849
BspPI GGATC 1 cut(s) 842
BspTNI GGTCTC 1 cut(s) 425
BsrFI RCCGGY 4 cut(s) 175, 402, 434, 461
BsrI ACTGG 1 cut(s) 45
BssAI RCCGGY 4 cut(s) 175, 402, 434, 461
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 5 cut(s) 325, 533, 576, 760, 834
Bst4CI ACNGT 3 cut(s) 122, 278, 611
Bst6I CTCTTC 2 cut(s) 810, 862
BstAPI GCANNNNNTGC 2 cut(s) 264, 517
BstC8I GCNNGC 4 cut(s) 167, 177, 284, 719
BstDEI CTNAG 2 cut(s) 615, 662
BstF5I GGATG 2 cut(s) 34, 826
BstFNI CGCG 4 cut(s) 111, 147, 224, 724
BstHHI GCGC 4 cut(s) 113, 149, 224, 726
BstKTI GATC 5 cut(s) 328, 536, 579, 763, 837
BstMAI GTCTC 2 cut(s) 415, 425
BstMBI GATC 5 cut(s) 325, 533, 576, 760, 834
BstMCI CGRYCG 1 cut(s) 96
BstMWI GCNNNNNNNGC 8 cut(s) 181, 249, 264, 270, 279, 511, 517, 723
BstNSI RCATGY 1 cut(s) 696
BstSFI CTRYAG 3 cut(s) 253, 265, 529
BstUI CGCG 4 cut(s) 111, 147, 224, 724
BstV1I GCAGC 6 cut(s) 251, 279, 282, 381, 523, 537
BstV2I GAAGAC 1 cut(s) 707
BstXI CCANNNNNNTGG 1 cut(s) 800
Bsu36I CCTNAGG 2 cut(s) 615, 662
BsuRI GGCC 5 cut(s) 5, 165, 179, 438, 721
BtgZI GCGATG 1 cut(s) 840
BtsCI GGATG 2 cut(s) 34, 826
BtsI GCAGTG 1 cut(s) 265
BtsIMutI CAGTG 2 cut(s) 265, 791
BveI ACCTGC 1 cut(s) 849
Cac8I GCNNGC 4 cut(s) 167, 177, 284, 719
CaiI CAGNNNCTG 1 cut(s) 264
CciI TCATGA 2 cut(s) 322, 328
CfoI GCGC 4 cut(s) 113, 149, 224, 726
Cfr10I RCCGGY 4 cut(s) 175, 402, 434, 461
Cfr13I GGNCC 5 cut(s) 4, 163, 178, 396, 437
Csp6I GTAC 4 cut(s) 27, 118, 300, 643
CviAII CATG 4 cut(s) 115, 323, 329, 693
CviQI GTAC 4 cut(s) 27, 118, 300, 643
DdeI CTNAG 2 cut(s) 615, 662
DpnI GATC 5 cut(s) 327, 535, 578, 762, 836
DpnII GATC 5 cut(s) 325, 533, 576, 760, 834
Eam1104I CTCTTC 2 cut(s) 810, 862
EarI CTCTTC 2 cut(s) 810, 862
EciI GGCGGA 1 cut(s) 725
Ecl136II GAGCTC 1 cut(s) 427
Eco24I GRGCYC 1 cut(s) 429
Eco31I GGTCTC 1 cut(s) 425
Eco47I GGWCC 1 cut(s) 396
Eco53kI GAGCTC 1 cut(s) 427
Eco81I CCTNAGG 2 cut(s) 615, 662
Eco88I CYCGRG 1 cut(s) 428
EcoICRI GAGCTC 1 cut(s) 427
EcoO109I RGGNCCY 2 cut(s) 163, 396
EcoRI GAATTC 1 cut(s) 769
EcoT22I ATGCAT 2 cut(s) 694, 853
EcoT38I GRGCYC 1 cut(s) 429
Esp3I CGTCTC 1 cut(s) 415
FaeI CATG 4 cut(s) 118, 326, 332, 696
FaqI GGGAC 2 cut(s) 205, 409
FatI CATG 4 cut(s) 114, 322, 328, 692
FauI CCCGC 1 cut(s) 17
FbaI TGATCA 3 cut(s) 325, 576, 760
Fnu4HI GCNGC 9 cut(s) 225, 228, 265, 268, 271, 370, 512, 526, 722
FokI GGATG 2 cut(s) 21, 833
FriOI GRGCYC 1 cut(s) 429
Fsp4HI GCNGC 9 cut(s) 225, 228, 265, 268, 271, 370, 512, 526, 722
FspBI CTAG 1 cut(s) 883
GlaI GCGC 4 cut(s) 112, 148, 223, 725
GluI GCNGC 9 cut(s) 225, 228, 265, 268, 271, 370, 512, 526, 722
GsuI CTGGAG 3 cut(s) 28, 365, 443
HaeIII GGCC 5 cut(s) 5, 165, 179, 438, 721
HapII CCGG 5 cut(s) 176, 403, 435, 445, 462
HhaI GCGC 4 cut(s) 113, 149, 224, 726
Hin1II CATG 4 cut(s) 118, 326, 332, 696
Hin6I GCGC 4 cut(s) 111, 147, 222, 724
HinP1I GCGC 4 cut(s) 111, 147, 222, 724
HinfI GANTC 3 cut(s) 352, 633, 866
HpaII CCGG 5 cut(s) 176, 403, 435, 445, 462
Hpy188I TCNGA 5 cut(s) 357, 415, 544, 731, 765
HpyAV CCTTC 3 cut(s) 78, 196, 578
HpyCH4III ACNGT 3 cut(s) 122, 278, 611
HpyCH4V TGCA 8 cut(s) 267, 478, 505, 511, 520, 692, 743, 851
HpyF10VI GCNNNNNNNGC 8 cut(s) 181, 249, 264, 270, 279, 511, 517, 723
HpyF3I CTNAG 2 cut(s) 615, 662
Hsp92II CATG 4 cut(s) 118, 326, 332, 696
HspAI GCGC 4 cut(s) 111, 147, 222, 724
KflI GGGWCCC 1 cut(s) 396
Kpn2I TCCGGA 1 cut(s) 444
KroI GCCGGC 1 cut(s) 175
KroNI GCCGGC 1 cut(s) 177
Ksp22I TGATCA 3 cut(s) 325, 576, 760
Kzo9I GATC 5 cut(s) 325, 533, 576, 760, 834
LmnI GCTCC 3 cut(s) 384, 424, 789
Lsp1109I GCAGC 6 cut(s) 251, 279, 282, 381, 523, 537
LweI GCATC 2 cut(s) 735, 838
MaeI CTAG 1 cut(s) 883
MaeIII GTNAC 2 cut(s) 122, 605
MalI GATC 5 cut(s) 327, 535, 578, 762, 836
MboI GATC 5 cut(s) 325, 533, 576, 760, 834
MboII GAAGA 7 cut(s) 397, 634, 712, 778, 821, 827, 849
MfeI CAATTG 1 cut(s) 515
MhlI GDGCHC 1 cut(s) 429
MluCI AATT 5 cut(s) 86, 515, 756, 769, 871
MmeI TCCRAC 1 cut(s) 812
Mph1103I ATGCAT 2 cut(s) 694, 853
MroI TCCGGA 1 cut(s) 444
MroNI GCCGGC 1 cut(s) 175
MroXI GAANNNNTTC 1 cut(s) 236
MseI TTAA 4 cut(s) 23, 486, 684, 878
MslI CAYNNNNRTG 1 cut(s) 327
MspA1I CMGCKG 1 cut(s) 252
MspI CCGG 5 cut(s) 176, 403, 435, 445, 462
MunI CAATTG 1 cut(s) 515
Mva1269I GAATGC 2 cut(s) 137, 851
MvnI CGCG 4 cut(s) 111, 147, 224, 724
MwoI GCNNNNNNNGC 8 cut(s) 181, 249, 264, 270, 279, 511, 517, 723
NaeI GCCGGC 1 cut(s) 177
NdeII GATC 5 cut(s) 325, 533, 576, 760, 834
NgoMIV GCCGGC 1 cut(s) 175
NlaIII CATG 4 cut(s) 118, 326, 332, 696
NlaIV GGNNCC 5 cut(s) 6, 397, 398, 439, 791
NmeAIII GCCGAG 2 cut(s) 198, 366
NmuCI GTSAC 1 cut(s) 122
NsiI ATGCAT 2 cut(s) 694, 853
NspI RCATGY 1 cut(s) 696
PaeR7I CTCGAG 1 cut(s) 428
PagI TCATGA 2 cut(s) 322, 328
PctI GAATGC 2 cut(s) 137, 851
PdiI GCCGGC 1 cut(s) 177
PdmI GAANNNNTTC 1 cut(s) 236
PfeI GAWTC 3 cut(s) 352, 633, 866
PkrI GCNGC 9 cut(s) 226, 229, 266, 269, 272, 371, 513, 527, 723
PpuMI RGGWCCY 1 cut(s) 396
Psp124BI GAGCTC 1 cut(s) 429
Psp5II RGGWCCY 1 cut(s) 396
PspN4I GGNNCC 5 cut(s) 6, 397, 398, 439, 791
PspPI GGNCC 5 cut(s) 4, 163, 178, 396, 437
PspPPI RGGWCCY 1 cut(s) 396
PstI CTGCAG 1 cut(s) 269
PstNI CAGNNNCTG 1 cut(s) 264
PvuII CAGCTG 1 cut(s) 252
RsaI GTAC 4 cut(s) 28, 119, 301, 644
RsaNI GTAC 4 cut(s) 27, 118, 300, 643
RseI CAYNNNNRTG 1 cut(s) 327
SacI GAGCTC 1 cut(s) 429
SaqAI TTAA 4 cut(s) 23, 486, 684, 878
SatI GCNGC 9 cut(s) 225, 228, 265, 268, 271, 370, 512, 526, 722
Sau3AI GATC 5 cut(s) 325, 533, 576, 760, 834
Sau96I GGNCC 5 cut(s) 4, 163, 178, 396, 437
SduI GDGCHC 1 cut(s) 429
SfaNI GCATC 2 cut(s) 735, 838
SfcI CTRYAG 3 cut(s) 253, 265, 529
Sfr274I CTCGAG 1 cut(s) 428
SgrAI CRCCGGYG 1 cut(s) 461
SinI GGWCC 1 cut(s) 396
SlaI CTCGAG 1 cut(s) 428
SmiMI CAYNNNNRTG 1 cut(s) 327
SmlI CTYRAG 1 cut(s) 428
SmoI CTYRAG 1 cut(s) 428
Sse9I AATT 5 cut(s) 86, 515, 756, 769, 871
SsiI CCGC 5 cut(s) 10, 224, 227, 710, 722
SspMI CTAG 1 cut(s) 883
SstI GAGCTC 1 cut(s) 429
TaaI ACNGT 3 cut(s) 122, 278, 611
TaqI TCGA 5 cut(s) 92, 159, 429, 636, 864
TaqII GACCGA 1 cut(s) 110
TasI AATT 5 cut(s) 86, 515, 756, 769, 871
TauI GCSGC 3 cut(s) 227, 230, 724
TfiI GAWTC 3 cut(s) 352, 633, 866
Tru1I TTAA 4 cut(s) 23, 486, 684, 878
Tru9I TTAA 4 cut(s) 23, 486, 684, 878
TscAI CASTG 2 cut(s) 265, 791
TseFI GTSAC 1 cut(s) 122
TseI GCWGC 6 cut(s) 264, 267, 270, 369, 511, 525
Tsp45I GTSAC 1 cut(s) 122
TspDTI ATGAA 3 cut(s) 311, 768, 828
TspRI CASTG 2 cut(s) 265, 791
VpaK11BI GGWCC 1 cut(s) 396
XapI RAATTY 3 cut(s) 86, 769, 871
XceI RCATGY 1 cut(s) 696
XhoI CTCGAG 1 cut(s) 428
XmnI GAANNNNTTC 1 cut(s) 236
XspI CTAG 1 cut(s) 883
Zsp2I ATGCAT 2 cut(s) 694, 853
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.