RLG00000035540

Zinc finger protein CONSTANS-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0003558
Physical Location & Seq
Forward (+)
68148730 .. 68151127
2398 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035540

Sequence Viewer

Length: 771 bp
ATGTACGGTCACAACGGCAACCCATTCCCACGCGCCAACGACTTCGAGGGCCTGCCAATGCCGGCCCTGCCCTTTGGGACTTACAACAATGACTACCACCACCTCGGCGCGGCGGCGAATGAGTTCAGCAACAACAGCTGTAGCAGCGGCTGCAGCAGCTACGGTGGCTCGCCCACCTATGCGAGTACCACTACCACTACGACTATTCATGATCATGAGGTTGAGCCGAGCCTGATGATTCAGAGGAGTGTGAGCAGCCACTCGCTCCAGAAGAACGTCGGGACCCACCGGCTTGTCTCGGACTTGCTTGAGCTCGAGACCGGCCCCGTCCGGAGGGTTTATAGCACCGGCGACTTGGATTTGCAGAGGATTAACAATACTAATGGGGTGCAAGTGCAGCAATTGCATAGCAGCTACAGATCATCATCAGAAAGTAGTCCATTGTCAAGCGAGAGCAGTATGATCATTGAAGGAATGAGCAAAGCCTGTCGTTACAGTCCTGAGGAGAAGAAAGAGAGAATCGAGAGGTACAGAAGCAAGAGAAACCTCAGGAACTTCAACAAAACGATTAAGTATGCATGTAGGAAGACATTGGCGGACAGCAGGCCGCGCATCAGAGGGCGATTTGCAAAGAATGATGAAATTGATCAGAAGAATTCCCCTAATGTTCAGTGGAGCCAAATGAGTGGGGAGGAAGATGAAGAGGATGGCGATGGTTGGATCAACCTGCTTGATGCATTCTCTTCAATCGAATCAAATTCCTTAATCTAG

Protein Analysis

257

Amino Acids

28.67

Weight (kDa)

6.08

Isoelectric Point (pI)

69.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCT PF06203 171 - 212 4.8e-19 CCT motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 735
AccII CGCG 3 cut(s) 33, 110, 610
AccIII TCCGGA 1 cut(s) 330
AciI CCGC 5 cut(s) 110, 113, 147, 596, 608
AclWI GGATC 1 cut(s) 728
AcsI RAATTY 2 cut(s) 655, 757
AfaI GTAC 3 cut(s) 5, 187, 530
AfiI CCNNNNNNNGG 2 cut(s) 109, 333
AgsI TTSAA 3 cut(s) 470, 559, 747
AluBI AGCT 4 cut(s) 138, 159, 313, 414
AluI AGCT 4 cut(s) 138, 159, 313, 414
Alw21I GWGCWC 1 cut(s) 315
Alw26I GTCTC 2 cut(s) 301, 311
AlwI GGATC 1 cut(s) 728
AlwNI CAGNNNCTG 1 cut(s) 150
Ama87I CYCGRG 1 cut(s) 314
Aor13HI TCCGGA 1 cut(s) 330
AoxI GGCC 4 cut(s) 49, 63, 322, 605
ApeKI GCWGC 7 cut(s) 144, 150, 153, 156, 255, 397, 411
ApoI RAATTY 2 cut(s) 655, 757
Asp700I GAANNNNTTC 1 cut(s) 122
AspLEI GCGC 3 cut(s) 35, 110, 612
AspS9I GGNCC 4 cut(s) 49, 64, 282, 323
AvaI CYCGRG 1 cut(s) 314
AvaII GGWCC 1 cut(s) 282
AxyI CCTNAGG 2 cut(s) 501, 548
BanII GRGCYC 1 cut(s) 315
BbsI GAAGAC 1 cut(s) 593
Bbv12I GWGCWC 1 cut(s) 315
BbvI GCAGC 7 cut(s) 137, 156, 165, 168, 267, 409, 423
BccI CCATC 2 cut(s) 701, 707
BceAI ACGGC 1 cut(s) 31
BclI TGATCA 3 cut(s) 211, 462, 646
BcoDI GTCTC 2 cut(s) 301, 311
BfaI CTAG 1 cut(s) 769
BfmI CTRYAG 3 cut(s) 139, 151, 415
BfuAI ACCTGC 1 cut(s) 735
Bme18I GGWCC 1 cut(s) 282
BmeT110I CYCGRG 1 cut(s) 314
BmgT120I GGNCC 4 cut(s) 49, 64, 282, 323
BmiI GGNNCC 4 cut(s) 283, 284, 325, 677
BmsI GCATC 2 cut(s) 621, 724
BpiI GAAGAC 1 cut(s) 593
BpmI CTGGAG 1 cut(s) 251
BpuEI CTTGAG 1 cut(s) 329
BsaBI GATNNNNATC 1 cut(s) 424
BsaI GGTCTC 1 cut(s) 311
BsaJI CCNNGG 1 cut(s) 103
BsaWI WCCGGW 1 cut(s) 330
Bsc4I CCNNNNNNNGG 2 cut(s) 109, 333
Bse118I RCCGGY 4 cut(s) 61, 288, 320, 347
Bse21I CCTNAGG 2 cut(s) 501, 548
Bse8I GATNNNNATC 1 cut(s) 424
BseAI TCCGGA 1 cut(s) 330
BseDI CCNNGG 1 cut(s) 103
BseGI GGATG 1 cut(s) 712
BseJI GATNNNNATC 1 cut(s) 424
BseLI CCNNNNNNNGG 2 cut(s) 109, 333
BseMII CTCAG 2 cut(s) 492, 562
BseRI GAGGAG 2 cut(s) 259, 518
BseXI GCAGC 7 cut(s) 137, 156, 165, 168, 267, 409, 423
BsgI GTGCAG 1 cut(s) 416
Bsh1236I CGCG 3 cut(s) 33, 110, 610
BshFI GGCC 4 cut(s) 51, 65, 324, 607
BsiHKAI GWGCWC 1 cut(s) 315
BsiHKCI CYCGRG 1 cut(s) 314
BsiSI CCGG 5 cut(s) 62, 289, 321, 331, 348
BslFI GGGAC 2 cut(s) 91, 295
BslI CCNNNNNNNGG 2 cut(s) 109, 333
BsmAI GTCTC 2 cut(s) 301, 311
BsmFI GGGAC 2 cut(s) 91, 295
BsmI GAATGC 1 cut(s) 737
BsnI GGCC 4 cut(s) 51, 65, 324, 607
Bso31I GGTCTC 1 cut(s) 311
BsoBI CYCGRG 1 cut(s) 314
Bsp1286I GDGCHC 1 cut(s) 315
Bsp13I TCCGGA 1 cut(s) 330
Bsp143I GATC 5 cut(s) 211, 419, 462, 646, 720
BspACI CCGC 5 cut(s) 110, 113, 147, 596, 608
BspANI GGCC 4 cut(s) 51, 65, 324, 607
BspCNI CTCAG 2 cut(s) 493, 561
BspEI TCCGGA 1 cut(s) 330
BspFNI CGCG 3 cut(s) 33, 110, 610
BspHI TCATGA 2 cut(s) 208, 214
BspLI GGNNCC 4 cut(s) 283, 284, 325, 677
BspMAI CTGCAG 1 cut(s) 155
BspMI ACCTGC 1 cut(s) 735
BspPI GGATC 1 cut(s) 728
BspTNI GGTCTC 1 cut(s) 311
BsrFI RCCGGY 4 cut(s) 61, 288, 320, 347
BssAI RCCGGY 4 cut(s) 61, 288, 320, 347
BssECI CCNNGG 1 cut(s) 103
BssMI GATC 5 cut(s) 211, 419, 462, 646, 720
Bst4CI ACNGT 3 cut(s) 8, 164, 497
Bst6I CTCTTC 2 cut(s) 696, 748
BstAPI GCANNNNNTGC 2 cut(s) 150, 403
BstC8I GCNNGC 4 cut(s) 53, 63, 170, 605
BstDEI CTNAG 2 cut(s) 501, 548
BstF5I GGATG 1 cut(s) 712
BstFNI CGCG 3 cut(s) 33, 110, 610
BstHHI GCGC 3 cut(s) 35, 110, 612
BstKTI GATC 5 cut(s) 214, 422, 465, 649, 723
BstMAI GTCTC 2 cut(s) 301, 311
BstMBI GATC 5 cut(s) 211, 419, 462, 646, 720
BstNSI RCATGY 1 cut(s) 582
BstSFI CTRYAG 3 cut(s) 139, 151, 415
BstUI CGCG 3 cut(s) 33, 110, 610
BstV1I GCAGC 7 cut(s) 137, 156, 165, 168, 267, 409, 423
BstV2I GAAGAC 1 cut(s) 593
BstXI CCANNNNNNTGG 1 cut(s) 686
Bsu36I CCTNAGG 2 cut(s) 501, 548
BsuRI GGCC 4 cut(s) 51, 65, 324, 607
BtgZI GCGATG 1 cut(s) 726
BtsCI GGATG 1 cut(s) 712
BtsIMutI CAGTG 1 cut(s) 677
BveI ACCTGC 1 cut(s) 735
Cac8I GCNNGC 4 cut(s) 53, 63, 170, 605
CaiI CAGNNNCTG 1 cut(s) 150
CciI TCATGA 2 cut(s) 208, 214
CfoI GCGC 3 cut(s) 35, 110, 612
Cfr10I RCCGGY 4 cut(s) 61, 288, 320, 347
Cfr13I GGNCC 4 cut(s) 49, 64, 282, 323
Csp6I GTAC 3 cut(s) 4, 186, 529
CviAII CATG 3 cut(s) 209, 215, 579
CviQI GTAC 3 cut(s) 4, 186, 529
DdeI CTNAG 2 cut(s) 501, 548
DpnI GATC 5 cut(s) 213, 421, 464, 648, 722
DpnII GATC 5 cut(s) 211, 419, 462, 646, 720
Eam1104I CTCTTC 2 cut(s) 696, 748
EarI CTCTTC 2 cut(s) 696, 748
EciI GGCGGA 1 cut(s) 611
Ecl136II GAGCTC 1 cut(s) 313
Eco24I GRGCYC 1 cut(s) 315
Eco31I GGTCTC 1 cut(s) 311
Eco47I GGWCC 1 cut(s) 282
Eco53kI GAGCTC 1 cut(s) 313
Eco81I CCTNAGG 2 cut(s) 501, 548
Eco88I CYCGRG 1 cut(s) 314
EcoICRI GAGCTC 1 cut(s) 313
EcoO109I RGGNCCY 2 cut(s) 49, 282
EcoRI GAATTC 1 cut(s) 655
EcoT22I ATGCAT 2 cut(s) 580, 739
EcoT38I GRGCYC 1 cut(s) 315
FaeI CATG 3 cut(s) 212, 218, 582
FaiI YATR 8 cut(s) 180, 210, 216, 342, 408, 461, 576, 580
FaqI GGGAC 2 cut(s) 91, 295
FatI CATG 3 cut(s) 208, 214, 578
FbaI TGATCA 3 cut(s) 211, 462, 646
FokI GGATG 1 cut(s) 719
FriOI GRGCYC 1 cut(s) 315
FspBI CTAG 1 cut(s) 769
GlaI GCGC 3 cut(s) 34, 109, 611
GsuI CTGGAG 1 cut(s) 251
HaeIII GGCC 4 cut(s) 51, 65, 324, 607
HapII CCGG 5 cut(s) 62, 289, 321, 331, 348
HhaI GCGC 3 cut(s) 35, 110, 612
Hin1II CATG 3 cut(s) 212, 218, 582
Hin6I GCGC 3 cut(s) 33, 108, 610
HinP1I GCGC 3 cut(s) 33, 108, 610
HinfI GANTC 3 cut(s) 238, 519, 752
HpaII CCGG 5 cut(s) 62, 289, 321, 331, 348
Hpy188I TCNGA 5 cut(s) 243, 301, 430, 617, 651
Hpy188III TCNNGA 9 cut(s) 209, 215, 268, 280, 316, 331, 500, 523, 550
Hpy99I CGWCG 1 cut(s) 281
HpyAV CCTTC 1 cut(s) 464
HpyCH4III ACNGT 3 cut(s) 8, 164, 497
HpyCH4IV ACGT 1 cut(s) 276
HpyCH4V TGCA 8 cut(s) 153, 364, 391, 397, 406, 578, 629, 737
HpyF3I CTNAG 2 cut(s) 501, 548
HpySE526I ACGT 1 cut(s) 276
Hsp92II CATG 3 cut(s) 212, 218, 582
HspAI GCGC 3 cut(s) 33, 108, 610
KflI GGGWCCC 1 cut(s) 282
Kpn2I TCCGGA 1 cut(s) 330
KroI GCCGGC 1 cut(s) 61
KroNI GCCGGC 1 cut(s) 63
Ksp22I TGATCA 3 cut(s) 211, 462, 646
Kzo9I GATC 5 cut(s) 211, 419, 462, 646, 720
LmnI GCTCC 2 cut(s) 270, 675
Lsp1109I GCAGC 7 cut(s) 137, 156, 165, 168, 267, 409, 423
LweI GCATC 2 cut(s) 621, 724
MaeI CTAG 1 cut(s) 769
MaeII ACGT 1 cut(s) 276
MaeIII GTNAC 2 cut(s) 8, 491
MalI GATC 5 cut(s) 213, 421, 464, 648, 722
MboI GATC 5 cut(s) 211, 419, 462, 646, 720
MboII GAAGA 7 cut(s) 283, 520, 598, 664, 707, 713, 735
MfeI CAATTG 1 cut(s) 401
MhlI GDGCHC 1 cut(s) 315
MluCI AATT 4 cut(s) 401, 642, 655, 757
MmeI TCCRAC 1 cut(s) 698
Mph1103I ATGCAT 2 cut(s) 580, 739
MroI TCCGGA 1 cut(s) 330
MroNI GCCGGC 1 cut(s) 61
MroXI GAANNNNTTC 1 cut(s) 122
MseI TTAA 3 cut(s) 372, 570, 764
MslI CAYNNNNRTG 1 cut(s) 213
MspA1I CMGCKG 2 cut(s) 138, 147
MspI CCGG 5 cut(s) 62, 289, 321, 331, 348
MunI CAATTG 1 cut(s) 401
Mva1269I GAATGC 1 cut(s) 737
MvnI CGCG 3 cut(s) 33, 110, 610
NaeI GCCGGC 1 cut(s) 63
NdeII GATC 5 cut(s) 211, 419, 462, 646, 720
NgoMIV GCCGGC 1 cut(s) 61
NlaIII CATG 3 cut(s) 212, 218, 582
NlaIV GGNNCC 4 cut(s) 283, 284, 325, 677
NmeAIII GCCGAG 2 cut(s) 84, 252
NmuCI GTSAC 1 cut(s) 8
NsiI ATGCAT 2 cut(s) 580, 739
NspI RCATGY 1 cut(s) 582
PaeR7I CTCGAG 1 cut(s) 314
PagI TCATGA 2 cut(s) 208, 214
PctI GAATGC 1 cut(s) 737
PdiI GCCGGC 1 cut(s) 63
PdmI GAANNNNTTC 1 cut(s) 122
PfeI GAWTC 3 cut(s) 238, 519, 752
PpuMI RGGWCCY 1 cut(s) 282
Psp124BI GAGCTC 1 cut(s) 315
Psp5II RGGWCCY 1 cut(s) 282
PspN4I GGNNCC 4 cut(s) 283, 284, 325, 677
PspPI GGNCC 4 cut(s) 49, 64, 282, 323
PspPPI RGGWCCY 1 cut(s) 282
PstI CTGCAG 1 cut(s) 155
PstNI CAGNNNCTG 1 cut(s) 150
PvuII CAGCTG 1 cut(s) 138
RsaI GTAC 3 cut(s) 5, 187, 530
RsaNI GTAC 3 cut(s) 4, 186, 529
RseI CAYNNNNRTG 1 cut(s) 213
SacI GAGCTC 1 cut(s) 315
SaqAI TTAA 3 cut(s) 372, 570, 764
Sau3AI GATC 5 cut(s) 211, 419, 462, 646, 720
Sau96I GGNCC 4 cut(s) 49, 64, 282, 323
SduI GDGCHC 1 cut(s) 315
SfaNI GCATC 2 cut(s) 621, 724
SfcI CTRYAG 3 cut(s) 139, 151, 415
Sfr274I CTCGAG 1 cut(s) 314
SgrAI CRCCGGYG 1 cut(s) 347
SinI GGWCC 1 cut(s) 282
SlaI CTCGAG 1 cut(s) 314
SmiMI CAYNNNNRTG 1 cut(s) 213
SmlI CTYRAG 2 cut(s) 308, 314
SmoI CTYRAG 2 cut(s) 308, 314
Sse9I AATT 4 cut(s) 401, 642, 655, 757
SsiI CCGC 5 cut(s) 110, 113, 147, 596, 608
SspMI CTAG 1 cut(s) 769
SstI GAGCTC 1 cut(s) 315
TaaI ACNGT 3 cut(s) 8, 164, 497
TaiI ACGT 1 cut(s) 279
TaqI TCGA 4 cut(s) 45, 315, 522, 750
TasI AATT 4 cut(s) 401, 642, 655, 757
TauI GCSGC 4 cut(s) 113, 116, 150, 610
TfiI GAWTC 3 cut(s) 238, 519, 752
Tru1I TTAA 3 cut(s) 372, 570, 764
Tru9I TTAA 3 cut(s) 372, 570, 764
TscAI CASTG 1 cut(s) 677
TseFI GTSAC 1 cut(s) 8
TseI GCWGC 7 cut(s) 144, 150, 153, 156, 255, 397, 411
Tsp45I GTSAC 1 cut(s) 8
TspDTI ATGAA 3 cut(s) 197, 654, 714
TspRI CASTG 1 cut(s) 677
VpaK11BI GGWCC 1 cut(s) 282
XapI RAATTY 2 cut(s) 655, 757
XceI RCATGY 1 cut(s) 582
XhoI CTCGAG 1 cut(s) 314
XmnI GAANNNNTTC 1 cut(s) 122
XspI CTAG 1 cut(s) 769
Zsp2I ATGCAT 2 cut(s) 580, 739
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.