RchiOBHm_Chr6g0255171

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0008180
Physical Location & Seq
Forward (+)
10463213 .. 10469284
6072 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22889

Sequence Viewer

Length: 2637 bp
ATGGCGTTGCCCGCTGTCGTCACTACTGCACTGGAATTAACTGGTGGAGGTTTCATCTCGTCGGCTTTGCAGCCTCCCTTTCAGAAACTCGCAAATTATATTTTCAGCCGGATAGAACGGACAAAAGATACCGGCGACAAGCTCCGAAAATTGGAAAGAACACTGTTGAAAATTCAATCTCTGATCGATCAGCTGGAAGGAAAGCACTCTACCTCAAAGGCTTGTCGACTTTTAATTGAAGATTTCAAGGGTGTTCTTCTTGATTCGGATGATTTTCTGAATGAGATTTGTCACCAATTGCCGAAAGACGGTCCATCATCCTTGGGTATACTTACCAGGGATAAGGTAGGTCTGACGGTTAAAGATGTGACTGATTTCTTCAATGAATTAGAGGGTTACTTCATCTCAGAAGTAACCAAGCCGGGGCCTCCTCAAGTCCCTCGTGGTAGTAGCTCCTTCATCGATCCGTCGTCTGTGATTGGATTGGAAGAAAAAAAGAAAAACATTAAGTTGTTGTTGGCTGCTGATGACAAGAATACTACTGTGATTTCAATAGTGGGTATGGCTGGCATCGGAAAAACAACACTTGTTCAACTTGTCTGTAATGATCCAGAGGTGATTGGGTGGTTTAACGAGAGAATATGGTGGGTATGTGTTTCTTGGGAGTATGATTTGATTCAGATCACGAAATCAATCCTTGAGGCTGCCACAAGCAGCGTTTGCACTCTGTCAAATCTCGACTCTATTATAAGGCAACTGAAAAAGGTGATGATGGAGAGGGGGAGGTTTTTACTCGTGCTGGACGATATGTGGAATGAGAGTCTAAGTCATTGGCGTGAGTTGAAATTGCTTTTCAGCTCTGCGGCACCTGGAAGTAGGGTTCTGGTAACCACTCAAAGGAAGACTGTTTCATCCATTGTTACCACTGATGGGGATCTTGGTTGTTTTCCGCTAACTACTTTGACCAAGGAGGATTGTTGGGAAATTATAAAGAAAAACCTGAAAACAGAAGTTCATAATCGTGAAGATTTGAAGGCTATTGGCTTGGAGTTAGCAGAGAAGTGTAAAGGTCTTCCTTTGGCGGCCAAAGTGATTGGAGATGCCTTGCATCTCAAATCAGAAGAGGTGGAGTGGGATGCGCTGTTGAAATGTCCTCCTTGGGACTGGCGTCACTTGCCAGAAGGTGAGAACCAAGTCTATCGCGTACTGAAGCCGAGCTATGTTCACTTGCCAGCTTACTTGACAAGATGCTTTGTTTACTTTTCTATTATCCCTCAAAGTCAAAAGGTCAAAGTGGAGGATTTGATCCAAATCTGGGCAGCGGAAGGCTTCACGGGAGCCCAAGGAGCAAGAAGAATTGAAGACATTGGAATGGAGTACTTGAAAGAATTGTGTTCGAGGTCTTTCATACAGATTTTGTCAGGTGAGATTAAAATGAACGACTTCATTCATGACTTGGCAACGTCGGTTTCTACCAATTTATGCTTGCGTTTGGAGGATACCATGTCTGCAAGATCTACCAGCTTCTTGTCTCAAAATGTGCGCTATTTGTCATTGCTTTGTCAAAGTGATGAATCAGATGTGTGTACAGCTAAGCTCAAAGAACTTTACAGGTATCAGAAATTGAGAACATTTATGTTCTTTTCCCATGTTAACCAACTCGCTACCAATCTTTCTATGTCAGATATCTTTCAAAAGTTTTTTGAGATATTCAAATGGTTAAGGGTTTTGAAGTTAACTGGTAGTCGCATCCATAAGTTGTCTGGCCCCATCGGCAAATTGAAGCGCCTTCGCTACCTCGATCTAAGTGATACTAAGATTGCAAAGTTGCCGCAGGAGATAGTAAATCTACTTGCGTTAGAAACACTTCGGCTCCAGAAATGTACTGAACTTAAAGAGTGGCCTAAGAACTTTGAGAAGTTGGTTAAGCTGCGACACCTTCATTTTGATAGACAGTGTCAAATGTGTTCCATGCCAAAAAACTTCGGGAAATTAACTAGTCTCGAAAACCTGACAACGGTTGAAGTTGAAAGTGAAAAAGGATATGGAATTGAGGAGCTTCAGAACATGAACTGCCTTCGTGGATCCATTGAGATTAAAAATCTCGAGAATGTGTTGGATGCCAAAAATGCTGAGGATGCTATGTTAGGTAAAAAGCAATACCTGCACAGGGTGAAGTTGCAATGGGATGAAACAAGAGATCAAATACTTGAAAAAAACTTGTTTCATCAACGAGTTATGAGCGGCCTTGAACCTCATTATAGGTTGAAAGAGTTGGAAGTGAAAGGCTATTGTGGCTCAATATTGTCAAACTGGATTGACAACCCACCGTCTGCCAACGCACCCACTCGTCAGATCGAGAAGCTTGTTCTTAGTTCTTGTCCACAATTAAGGAAATTATCTTCAATTTGTCACCTAACTTCGCTTAAAACATTAGAGATCCTCGATTGCCCCAAGCTTAACTCATTGCCAGAGAGAGGACTACCTAGCTCACTTGTGACTTTGAACATTTCAGAAAGTGGCATCTCAAGACGGCAATGTGAGGACGGAGGCAGCATGCGGGAAACTGTCAGATTTATTCTCGATGTACATATTAATGGTCACAAAATTCCAACGGAACGTTTGCCACGGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

878

Amino Acids

99.79

Weight (kDa)

8.35

Isoelectric Point (pI)

39.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 24 - 102 3.9e-10 Rx N-terminal domain
NB-ARC PF00931 166 - 333 1.4e-29 NB-ARC domain
WHD_DRP PF23559 423 - 487 1.7e-15 Disease resistance protein Winged helix domain
LRR_14 PF23598 568 - 777 3.7e-27 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 684 - 816 7.5e-29 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 749, 989
Acc36I ACCTGC 1 cut(s) 2172
AccB1I GGYRCC 1 cut(s) 865
AccBSI CCGCTC 1 cut(s) 2244
AccI GTMKAC 2 cut(s) 226, 328
AccII CGCG 1 cut(s) 1203
AciI CCGC 8 cut(s) 12, 863, 950, 1082, 1322, 1832, 2244, 2560
AclI AACGTT 1 cut(s) 2620
AclWI GGATC 7 cut(s) 458, 602, 942, 1300, 2079, 2092, 2434
AcoI YGGCCR 1 cut(s) 1083
AcsI RAATTY 2 cut(s) 171, 2607
AcuI CTGAAG 2 cut(s) 1229, 2045
AcyI GRCGYC 1 cut(s) 1168
AdeI CACNNNGTG 1 cut(s) 2173
AfaI GTAC 5 cut(s) 1206, 1379, 1588, 1885, 2589
AfiI CCNNNNNNNGG 9 cut(s) 151, 308, 423, 897, 930, 1315, 2017, 2170, 2477
AhlI ACTAGT 1 cut(s) 1997
AjnI CCWGG 2 cut(s) 335, 868
AloI GAACNNNNNNTCC 2 cut(s) 864, 896
Alw26I GTCTC 2 cut(s) 1536, 2006
AlwI GGATC 7 cut(s) 458, 602, 942, 1300, 2079, 2092, 2434
Ama87I CYCGRG 1 cut(s) 2105
AoxI GGCC 5 cut(s) 425, 1083, 1765, 1901, 2245
ApeKI GCWGC 7 cut(s) 70, 521, 704, 714, 1319, 1930, 2553
ApoI RAATTY 2 cut(s) 171, 2607
AseI ATTAAT 1 cut(s) 2595
Asp700I GAANNNNTTC 2 cut(s) 1442, 1866
AspLEI GCGC 3 cut(s) 1141, 1545, 1788
AspS9I GGNCC 3 cut(s) 311, 425, 1766
AsuC2I CCSGG 1 cut(s) 423
AsuHPI GGTGA 7 cut(s) 284, 628, 778, 1196, 1436, 2185, 2405
AvaI CYCGRG 1 cut(s) 2105
AvaII GGWCC 1 cut(s) 311
BamHI GGATCC 1 cut(s) 2084
BanI GGYRCC 1 cut(s) 865
BanII GRGCYC 1 cut(s) 1342
BauI CACGAG 2 cut(s) 441, 794
BbsI GAAGAC 3 cut(s) 908, 1064, 1368
BbvCI CCTCAGC 1 cut(s) 2133
BbvI GCAGC 7 cut(s) 82, 508, 691, 726, 1331, 1917, 2565
BccI CCATC 4 cut(s) 322, 766, 923, 1778
BceAI ACGGC 1 cut(s) 2549
BciT130I CCWGG 2 cut(s) 337, 870
BciVI GTATCC 1 cut(s) 1492
BcnI CCSGG 1 cut(s) 423
BcoDI GTCTC 2 cut(s) 1536, 2006
BcuI ACTAGT 1 cut(s) 1997
BfaI CTAG 2 cut(s) 1998, 2487
BfoI RGCGCY 1 cut(s) 1789
BfuAI ACCTGC 1 cut(s) 2172
BfuI GTATCC 1 cut(s) 1492
BglI GCCNNNNNGGC 1 cut(s) 1773
BglII AGATCT 1 cut(s) 1514
BlpI GCTNAGC 1 cut(s) 1593
BmcAI AGTACT 1 cut(s) 1379
Bme1390I CCNGG 3 cut(s) 337, 423, 870
Bme18I GGWCC 1 cut(s) 311
BmeT110I CYCGRG 1 cut(s) 2105
BmgT120I GGNCC 3 cut(s) 311, 425, 1766
BmiI GGNNCC 6 cut(s) 426, 867, 1339, 1768, 1874, 2086
BmrFI CCNGG 3 cut(s) 337, 423, 870
BmsI GCATC 9 cut(s) 579, 1090, 1117, 1126, 1238, 1758, 2110, 2128, 2532
BoxI GACNNNNGTC 1 cut(s) 1167
BpiI GAAGAC 3 cut(s) 908, 1064, 1368
BpmI CTGGAG 1 cut(s) 1859
Bpu10I CCTNAGC 1 cut(s) 2133
Bpu1102I GCTNAGC 1 cut(s) 1593
BpuEI CTTGAG 3 cut(s) 417, 719, 2512
BpuMI CCSGG 1 cut(s) 423
Bsa29I ATCGAT 2 cut(s) 186, 462
BsaBI GATNNNNATC 3 cut(s) 680, 933, 1310
BsaHI GRCGYC 1 cut(s) 1168
BsaJI CCNNGG 7 cut(s) 321, 336, 422, 966, 1157, 1342, 2627
BsaXI ACNNNNNCTCC 2 cut(s) 1857, 1887
Bsc4I CCNNNNNNNGG 9 cut(s) 151, 308, 423, 897, 930, 1315, 2017, 2170, 2477
Bse118I RCCGGY 1 cut(s) 131
Bse1I ACTGG 5 cut(s) 36, 46, 1169, 1744, 2318
Bse3DI GCAATG 4 cut(s) 1553, 2189, 2465, 2543
Bse8I GATNNNNATC 3 cut(s) 680, 933, 1310
BseBI CCWGG 2 cut(s) 337, 870
BseCI ATCGAT 2 cut(s) 186, 462
BseDI CCNNGG 7 cut(s) 321, 336, 422, 966, 1157, 1342, 2627
BseGI GGATG 8 cut(s) 274, 317, 911, 1141, 1749, 2125, 2143, 2194
BseJI GATNNNNATC 3 cut(s) 680, 933, 1310
BseLI CCNNNNNNNGG 9 cut(s) 151, 308, 423, 897, 930, 1315, 2017, 2170, 2477
BseMI GCAATG 4 cut(s) 1553, 2189, 2465, 2543
BseMII CTCAG 2 cut(s) 420, 2124
BseNI ACTGG 5 cut(s) 36, 46, 1169, 1744, 2318
BseRI GAGGAG 2 cut(s) 420, 2069
BseXI GCAGC 7 cut(s) 82, 508, 691, 726, 1331, 1917, 2565
BsgI GTGCAG 2 cut(s) 12, 2150
Bsh1236I CGCG 1 cut(s) 1203
BshFI GGCC 5 cut(s) 427, 1085, 1767, 1903, 2247
BshNI GGYRCC 1 cut(s) 865
BshVI ATCGAT 2 cut(s) 186, 462
BsiHKCI CYCGRG 1 cut(s) 2105
BsiSI CCGG 3 cut(s) 109, 132, 422
BslFI GGGAC 2 cut(s) 422, 1175
BslI CCNNNNNNNGG 9 cut(s) 151, 308, 423, 897, 930, 1315, 2017, 2170, 2477
BsmAI GTCTC 2 cut(s) 1536, 2006
BsmFI GGGAC 2 cut(s) 422, 1175
BsnI GGCC 5 cut(s) 427, 1085, 1767, 1903, 2247
BsoBI CYCGRG 1 cut(s) 2105
Bsp1286I GDGCHC 1 cut(s) 1342
Bsp1407I TGTACA 2 cut(s) 1586, 2587
Bsp1720I GCTNAGC 1 cut(s) 1593
BspACI CCGC 8 cut(s) 12, 863, 950, 1082, 1322, 1832, 2244, 2560
BspANI GGCC 5 cut(s) 427, 1085, 1767, 1903, 2247
BspCNI CTCAG 2 cut(s) 419, 2125
BspDI ATCGAT 2 cut(s) 186, 462
BspFNI CGCG 1 cut(s) 1203
BspHI TCATGA 1 cut(s) 1450
BspLI GGNNCC 6 cut(s) 426, 867, 1339, 1768, 1874, 2086
BspMI ACCTGC 1 cut(s) 2172
BspPI GGATC 7 cut(s) 458, 602, 942, 1300, 2079, 2092, 2434
BspT107I GGYRCC 1 cut(s) 865
BsrBI CCGCTC 1 cut(s) 2244
BsrDI GCAATG 4 cut(s) 1553, 2189, 2465, 2543
BsrFI RCCGGY 1 cut(s) 131
BsrGI TGTACA 2 cut(s) 1586, 2587
BsrI ACTGG 5 cut(s) 36, 46, 1169, 1744, 2318
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 7 cut(s) 321, 336, 422, 966, 1157, 1342, 2627
BssNAI GTATAC 1 cut(s) 329
BssNI GRCGYC 1 cut(s) 1168
BssSI CACGAG 2 cut(s) 441, 794
BssT1I CCWWGG 4 cut(s) 321, 966, 1157, 1342
Bst1107I GTATAC 1 cut(s) 329
Bst2BI CACGAG 2 cut(s) 441, 794
Bst2UI CCWGG 2 cut(s) 337, 870
Bst4CI ACNGT 9 cut(s) 165, 311, 358, 544, 907, 1956, 2020, 2331, 2569
Bst6I CTCTTC 1 cut(s) 1116
BstACI GRCGYC 1 cut(s) 1168
BstAPI GCANNNNNTGC 2 cut(s) 720, 2164
BstAUI TGTACA 2 cut(s) 1586, 2587
BstC8I GCNNGC 5 cut(s) 12, 568, 1233, 1487, 2558
BstDEI CTNAG 8 cut(s) 406, 824, 1593, 1805, 1815, 1905, 2133, 2372
BstDSI CCRYGG 1 cut(s) 2627
BstEII GGTNACC 1 cut(s) 886
BstF5I GGATG 8 cut(s) 274, 317, 911, 1141, 1749, 2125, 2143, 2194
BstFNI CGCG 1 cut(s) 1203
BstH2I RGCGCY 1 cut(s) 1789
BstHHI GCGC 3 cut(s) 1141, 1545, 1788
BstMAI GTCTC 2 cut(s) 1536, 2006
BstMWI GCNNNNNNNGC 9 cut(s) 11, 720, 1174, 1346, 1773, 2129, 2138, 2164, 2295
BstNI CCWGG 2 cut(s) 337, 870
BstNSI RCATGY 1 cut(s) 2560
BstPAI GACNNNNGTC 1 cut(s) 1167
BstPI GGTNACC 1 cut(s) 886
BstSCI CCNGG 3 cut(s) 335, 421, 868
BstUI CGCG 1 cut(s) 1203
BstV1I GCAGC 7 cut(s) 82, 508, 691, 726, 1331, 1917, 2565
BstV2I GAAGAC 3 cut(s) 908, 1064, 1368
BstX2I RGATCY 4 cut(s) 934, 1514, 2084, 2439
BstYI RGATCY 4 cut(s) 934, 1514, 2084, 2439
BstZ17I GTATAC 1 cut(s) 329
Bsu15I ATCGAT 2 cut(s) 186, 462
BsuI GTATCC 1 cut(s) 1492
BsuRI GGCC 5 cut(s) 427, 1085, 1767, 1903, 2247
BsuTUI ATCGAT 2 cut(s) 186, 462
BtgI CCRYGG 1 cut(s) 2627
BtsCI GGATG 8 cut(s) 274, 317, 911, 1141, 1749, 2125, 2143, 2194
BtsIMutI CAGTG 4 cut(s) 29, 161, 924, 1961
BveI ACCTGC 1 cut(s) 2172
Cac8I GCNNGC 5 cut(s) 12, 568, 1233, 1487, 2558
CciI TCATGA 1 cut(s) 1450
CfoI GCGC 3 cut(s) 1141, 1545, 1788
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 3 cut(s) 311, 425, 1766
ClaI ATCGAT 2 cut(s) 186, 462
CseI GACGC 1 cut(s) 1157
Csp6I GTAC 5 cut(s) 1205, 1378, 1587, 1884, 2588
CviAII CATG 6 cut(s) 1451, 1504, 1649, 1972, 2068, 2557
CviQI GTAC 5 cut(s) 1205, 1378, 1587, 1884, 2588
DdeI CTNAG 8 cut(s) 406, 824, 1593, 1805, 1815, 1905, 2133, 2372
DraIII CACNNNGTG 1 cut(s) 2173
EaeI YGGCCR 1 cut(s) 1083
Eam1104I CTCTTC 1 cut(s) 1116
EarI CTCTTC 1 cut(s) 1116
Eco130I CCWWGG 4 cut(s) 321, 966, 1157, 1342
Eco24I GRGCYC 1 cut(s) 1342
Eco32I GATATC 1 cut(s) 1687
Eco47I GGWCC 1 cut(s) 311
Eco57I CTGAAG 2 cut(s) 1229, 2045
Eco88I CYCGRG 1 cut(s) 2105
Eco91I GGTNACC 1 cut(s) 886
EcoO109I RGGNCCY 1 cut(s) 425
EcoO65I GGTNACC 1 cut(s) 886
EcoRII CCWGG 2 cut(s) 335, 868
EcoRV GATATC 1 cut(s) 1687
EcoT14I CCWWGG 4 cut(s) 321, 966, 1157, 1342
EcoT38I GRGCYC 1 cut(s) 1342
ErhI CCWWGG 4 cut(s) 321, 966, 1157, 1342
FaeI CATG 6 cut(s) 1454, 1507, 1652, 1975, 2071, 2560
FaqI GGGAC 2 cut(s) 422, 1175
FatI CATG 6 cut(s) 1450, 1503, 1648, 1971, 2067, 2556
FauI CCCGC 2 cut(s) 19, 2553
FblI GTMKAC 2 cut(s) 226, 328
FokI GGATG 8 cut(s) 281, 304, 898, 1148, 1736, 2132, 2150, 2201
FriOI GRGCYC 1 cut(s) 1342
FspBI CTAG 2 cut(s) 1998, 2487
GlaI GCGC 3 cut(s) 1140, 1544, 1787
GsuI CTGGAG 1 cut(s) 1859
HaeII RGCGCY 1 cut(s) 1789
HaeIII GGCC 5 cut(s) 427, 1085, 1767, 1903, 2247
HapII CCGG 3 cut(s) 109, 132, 422
HgaI GACGC 1 cut(s) 1157
HhaI GCGC 3 cut(s) 1141, 1545, 1788
Hin1I GRCGYC 1 cut(s) 1168
Hin1II CATG 6 cut(s) 1454, 1507, 1652, 1975, 2071, 2560
Hin6I GCGC 3 cut(s) 1139, 1543, 1786
HinP1I GCGC 3 cut(s) 1139, 1543, 1786
HincII GTYRAC 3 cut(s) 227, 1654, 1737
HindII GTYRAC 3 cut(s) 227, 1654, 1737
HindIII AAGCTT 2 cut(s) 2363, 2456
HinfI GANTC 5 cut(s) 263, 676, 740, 820, 1574
HpaI GTTAAC 2 cut(s) 1654, 1737
HpaII CCGG 3 cut(s) 109, 132, 422
HphI GGTGA 7 cut(s) 284, 628, 778, 1196, 1436, 2185, 2405
Hpy166II GTNNAC 8 cut(s) 227, 329, 1225, 1258, 1587, 1654, 1737, 2384
Hpy8I GTNNAC 8 cut(s) 227, 329, 1225, 1258, 1587, 1654, 1737, 2384
Hpy99I CGWCG 3 cut(s) 64, 472, 1468
HpyAV CCTTC 8 cut(s) 191, 466, 1027, 1175, 1319, 1799, 1949, 2087
HpyCH4III ACNGT 9 cut(s) 165, 311, 358, 544, 907, 1956, 2020, 2331, 2569
HpyCH4IV ACGT 2 cut(s) 1463, 2620
HpyCH4V TGCA 8 cut(s) 29, 70, 723, 1108, 1511, 1823, 2167, 2182
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 720, 1174, 1346, 1773, 2129, 2138, 2164, 2295
HpyF3I CTNAG 8 cut(s) 406, 824, 1593, 1805, 1815, 1905, 2133, 2372
HpySE526I ACGT 2 cut(s) 1463, 2620
Hsp92I GRCGYC 1 cut(s) 1168
Hsp92II CATG 6 cut(s) 1454, 1507, 1652, 1975, 2071, 2560
HspAI GCGC 3 cut(s) 1139, 1543, 1786
KspAI GTTAAC 2 cut(s) 1654, 1737
LmnI GCTCC 6 cut(s) 147, 458, 1337, 1346, 1878, 2056
Lsp1109I GCAGC 7 cut(s) 82, 508, 691, 726, 1331, 1917, 2565
LweI GCATC 9 cut(s) 579, 1090, 1117, 1126, 1238, 1758, 2110, 2128, 2532
MaeI CTAG 2 cut(s) 1998, 2487
MaeII ACGT 2 cut(s) 1463, 2620
MbiI CCGCTC 1 cut(s) 2244
MfeI CAATTG 1 cut(s) 296
MflI RGATCY 4 cut(s) 934, 1514, 2084, 2439
MhlI GDGCHC 1 cut(s) 1342
MlyI GAGTC 2 cut(s) 734, 829
MmeI TCCRAC 3 cut(s) 2097, 2256, 2636
MroXI GAANNNNTTC 2 cut(s) 1442, 1866
MslI CAYNNNNRTG 5 cut(s) 834, 1020, 1370, 2595, 2632
MspA1I CMGCKG 3 cut(s) 14, 193, 1322
MspI CCGG 3 cut(s) 109, 132, 422
MspR9I CCNGG 3 cut(s) 337, 423, 870
MunI CAATTG 1 cut(s) 296
MvaI CCWGG 2 cut(s) 337, 870
MvnI CGCG 1 cut(s) 1203
MwoI GCNNNNNNNGC 9 cut(s) 11, 720, 1174, 1346, 1773, 2129, 2138, 2164, 2295
NciI CCSGG 1 cut(s) 423
NlaIII CATG 6 cut(s) 1454, 1507, 1652, 1975, 2071, 2560
NlaIV GGNNCC 6 cut(s) 426, 867, 1339, 1768, 1874, 2086
NmeAIII GCCGAG 1 cut(s) 1239
NmuCI GTSAC 7 cut(s) 19, 290, 367, 1169, 2411, 2497, 2600
NspI RCATGY 1 cut(s) 2560
PaeI GCATGC 1 cut(s) 2560
PaeR7I CTCGAG 1 cut(s) 2105
PagI TCATGA 1 cut(s) 1450
PcsI WCGNNNNNNNCGW 1 cut(s) 801
PdmI GAANNNNTTC 2 cut(s) 1442, 1866
PfeI GAWTC 3 cut(s) 263, 676, 1574
PflFI GACNNNGTC 1 cut(s) 1956
PleI GAGTC 2 cut(s) 734, 828
PpsI GAGTC 2 cut(s) 734, 828
PshAI GACNNNNGTC 1 cut(s) 1167
PshBI ATTAAT 1 cut(s) 2595
PsiI TTATAA 2 cut(s) 749, 989
Psp1406I AACGTT 1 cut(s) 2620
Psp6I CCWGG 2 cut(s) 335, 868
PspEI GGTNACC 1 cut(s) 886
PspGI CCWGG 2 cut(s) 335, 868
PspN4I GGNNCC 6 cut(s) 426, 867, 1339, 1768, 1874, 2086
PspPI GGNCC 3 cut(s) 311, 425, 1766
PsuI RGATCY 4 cut(s) 934, 1514, 2084, 2439
PsyI GACNNNGTC 1 cut(s) 1956
PvuII CAGCTG 1 cut(s) 193
RsaI GTAC 5 cut(s) 1206, 1379, 1588, 1885, 2589
RsaNI GTAC 5 cut(s) 1205, 1378, 1587, 1884, 2588
RseI CAYNNNNRTG 5 cut(s) 834, 1020, 1370, 2595, 2632
SalI GTCGAC 1 cut(s) 225
Sau96I GGNCC 3 cut(s) 311, 425, 1766
ScaI AGTACT 1 cut(s) 1379
SchI GAGTC 2 cut(s) 734, 829
ScrFI CCNGG 3 cut(s) 337, 423, 870
SduI GDGCHC 1 cut(s) 1342
SfaNI GCATC 9 cut(s) 579, 1090, 1117, 1126, 1238, 1758, 2110, 2128, 2532
Sfr274I CTCGAG 1 cut(s) 2105
SinI GGWCC 1 cut(s) 311
SlaI CTCGAG 1 cut(s) 2105
SmiMI CAYNNNNRTG 5 cut(s) 834, 1020, 1370, 2595, 2632
SmlI CTYRAG 4 cut(s) 432, 698, 2105, 2527
SmoI CTYRAG 4 cut(s) 432, 698, 2105, 2527
SpeI ACTAGT 1 cut(s) 1997
SphI GCATGC 1 cut(s) 2560
SsiI CCGC 8 cut(s) 12, 863, 950, 1082, 1322, 1832, 2244, 2560
SspI AATATT 1 cut(s) 2304
SspMI CTAG 2 cut(s) 1998, 2487
StyD4I CCNGG 3 cut(s) 335, 421, 868
StyI CCWWGG 4 cut(s) 321, 966, 1157, 1342
TaaI ACNGT 9 cut(s) 165, 311, 358, 544, 907, 1956, 2020, 2331, 2569
TaiI ACGT 2 cut(s) 1466, 2623
TatI WGTACW 4 cut(s) 1377, 1586, 1883, 2587
TauI GCSGC 4 cut(s) 866, 1085, 1834, 2247
TfiI GAWTC 3 cut(s) 263, 676, 1574
TscAI CASTG 4 cut(s) 36, 168, 931, 1961
TseFI GTSAC 7 cut(s) 19, 290, 367, 1169, 2411, 2497, 2600
TseI GCWGC 7 cut(s) 70, 521, 704, 714, 1319, 1930, 2553
Tsp45I GTSAC 7 cut(s) 19, 290, 367, 1169, 2411, 2497, 2600
TspGWI ACGGA 4 cut(s) 133, 456, 2562, 2630
TspRI CASTG 4 cut(s) 36, 168, 931, 1961
Tth111I GACNNNGTC 1 cut(s) 1956
VpaK11BI GGWCC 1 cut(s) 311
VspI ATTAAT 1 cut(s) 2595
XapI RAATTY 2 cut(s) 171, 2607
XceI RCATGY 1 cut(s) 2560
XcmI CCANNNNNNNNNTGG 1 cut(s) 1760
XhoI CTCGAG 1 cut(s) 2105
XmiI GTMKAC 2 cut(s) 226, 328
XmnI GAANNNNTTC 2 cut(s) 1442, 1866
XspI CTAG 2 cut(s) 1998, 2487
ZrmI AGTACT 1 cut(s) 1379
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.