RchiOBHm_Chr6g0262301

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0008889
Physical Location & Seq
Reverse (-)
16995010 .. 16995736
727 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23522

Sequence Viewer

Length: 345 bp
ATGAAGACGTCTGATGACTCTGACCAGATTGAGACGTCTGATCATGTGTGGATGCGCTACATTCCATTCGTTGAGTTCGGGTTTCATCAGCCGCCTTTTACCTGTCGTGTTATTTTGGAACATTATAGCCAAGATTATTCGGTGCGCTGTACAAGCTATGGGGTCCACCTGGTAATGCCAATGGTGAACGATATGAAGATGGTAAACAATACGAAGATGATGATTGATGATGAATATGGTGAAAGCATTCCAGGTACGAGCAACAGAAATAAGACTTCTCCAGACGAATATCTTGTCCACCGCCAAAGAAGCAATTCCTCGTCCAAAACACTAGTTTTGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

114

Amino Acids

13.29

Weight (kDa)

5.64

Isoelectric Point (pI)

55.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 11, 38
AciI CCGC 2 cut(s) 92, 301
AcyI GRCGYC 2 cut(s) 8, 35
AfaI GTAC 2 cut(s) 151, 256
AgsI TTSAA 1 cut(s) 340
AhlI ACTAGT 1 cut(s) 331
AjnI CCWGG 2 cut(s) 168, 250
AluBI AGCT 1 cut(s) 156
AluI AGCT 1 cut(s) 156
Alw26I GTCTC 1 cut(s) 26
ArsI GACNNNNNNTTYG 2 cut(s) 279, 311
Asp700I GAANNNNTTC 2 cut(s) 246, 313
AspLEI GCGC 2 cut(s) 57, 147
AspS9I GGNCC 1 cut(s) 163
AsuHPI GGTGA 2 cut(s) 196, 251
AvaII GGWCC 1 cut(s) 163
BbsI GAAGAC 1 cut(s) 11
BccI CCATC 1 cut(s) 193
BciT130I CCWGG 2 cut(s) 170, 252
BclI TGATCA 1 cut(s) 40
BcoDI GTCTC 1 cut(s) 26
BcuI ACTAGT 1 cut(s) 331
BfaI CTAG 1 cut(s) 332
BisI GCNGC 1 cut(s) 92
BlsI GCNGC 1 cut(s) 93
Bme1390I CCNGG 2 cut(s) 170, 252
Bme18I GGWCC 1 cut(s) 163
BmgT120I GGNCC 1 cut(s) 163
BmiI GGNNCC 1 cut(s) 164
BmrFI CCNGG 2 cut(s) 170, 252
BmsI GCATC 1 cut(s) 42
BpiI GAAGAC 1 cut(s) 11
BpmI CTGGAG 1 cut(s) 264
BsaHI GRCGYC 2 cut(s) 8, 35
BseBI CCWGG 2 cut(s) 170, 252
BseGI GGATG 1 cut(s) 57
BsmAI GTCTC 1 cut(s) 26
BsmBI CGTCTC 1 cut(s) 26
BsmI GAATGC 1 cut(s) 246
Bsp1407I TGTACA 1 cut(s) 149
Bsp143I GATC 1 cut(s) 40
BspACI CCGC 2 cut(s) 92, 301
BspLI GGNNCC 1 cut(s) 164
BsrGI TGTACA 1 cut(s) 149
BssMI GATC 1 cut(s) 40
BssNI GRCGYC 2 cut(s) 8, 35
Bst2UI CCWGG 2 cut(s) 170, 252
BstACI GRCGYC 2 cut(s) 8, 35
BstAUI TGTACA 1 cut(s) 149
BstF5I GGATG 1 cut(s) 57
BstHHI GCGC 2 cut(s) 57, 147
BstKTI GATC 1 cut(s) 43
BstMAI GTCTC 1 cut(s) 26
BstMBI GATC 1 cut(s) 40
BstMWI GCNNNNNNNGC 2 cut(s) 153, 309
BstNI CCWGG 2 cut(s) 170, 252
BstSCI CCNGG 2 cut(s) 168, 250
BstV2I GAAGAC 1 cut(s) 11
BtsCI GGATG 1 cut(s) 57
CfoI GCGC 2 cut(s) 57, 147
Cfr13I GGNCC 1 cut(s) 163
CsiI ACCWGGT 1 cut(s) 168
Csp6I GTAC 2 cut(s) 150, 255
CviAII CATG 1 cut(s) 44
CviJI RGCY 3 cut(s) 91, 129, 156
CviKI_1 RGCY 3 cut(s) 91, 129, 156
CviQI GTAC 2 cut(s) 150, 255
DpnI GATC 1 cut(s) 42
DpnII GATC 1 cut(s) 40
Eco47I GGWCC 1 cut(s) 163
EcoRII CCWGG 2 cut(s) 168, 250
Esp3I CGTCTC 1 cut(s) 26
FaeI CATG 1 cut(s) 47
FaiI YATR 5 cut(s) 45, 126, 159, 194, 237
FatI CATG 1 cut(s) 43
FbaI TGATCA 1 cut(s) 40
Fnu4HI GCNGC 1 cut(s) 92
FokI GGATG 1 cut(s) 64
Fsp4HI GCNGC 1 cut(s) 92
FspBI CTAG 1 cut(s) 332
GlaI GCGC 2 cut(s) 56, 146
GluI GCNGC 1 cut(s) 92
GsuI CTGGAG 1 cut(s) 264
HhaI GCGC 2 cut(s) 57, 147
Hin1I GRCGYC 2 cut(s) 8, 35
Hin1II CATG 1 cut(s) 47
Hin6I GCGC 2 cut(s) 55, 145
HinP1I GCGC 2 cut(s) 55, 145
HinfI GANTC 1 cut(s) 17
HphI GGTGA 2 cut(s) 196, 251
Hpy166II GTNNAC 4 cut(s) 166, 187, 205, 298
Hpy188I TCNGA 3 cut(s) 13, 22, 40
Hpy188III TCNNGA 1 cut(s) 281
Hpy8I GTNNAC 4 cut(s) 166, 187, 205, 298
HpyCH4IV ACGT 2 cut(s) 8, 35
HpyF10VI GCNNNNNNNGC 2 cut(s) 153, 309
HpySE526I ACGT 2 cut(s) 8, 35
Hsp92I GRCGYC 2 cut(s) 8, 35
Hsp92II CATG 1 cut(s) 47
HspAI GCGC 2 cut(s) 55, 145
Ksp22I TGATCA 1 cut(s) 40
Kzo9I GATC 1 cut(s) 40
LpnPI CCDG 7 cut(s) 38, 115, 155, 182, 237, 264, 294
LweI GCATC 1 cut(s) 42
MabI ACCWGGT 1 cut(s) 168
MaeI CTAG 1 cut(s) 332
MaeII ACGT 2 cut(s) 8, 35
MalI GATC 1 cut(s) 42
MboI GATC 1 cut(s) 40
MboII GAAGA 3 cut(s) 16, 208, 226
MluCI AATT 2 cut(s) 313, 340
MlyI GAGTC 1 cut(s) 11
MnlI CCTC 1 cut(s) 328
MroXI GAANNNNTTC 2 cut(s) 246, 313
MspR9I CCNGG 2 cut(s) 170, 252
Mva1269I GAATGC 1 cut(s) 246
MvaI CCWGG 2 cut(s) 170, 252
MwoI GCNNNNNNNGC 2 cut(s) 153, 309
NdeII GATC 1 cut(s) 40
NlaIII CATG 1 cut(s) 47
NlaIV GGNNCC 1 cut(s) 164
PctI GAATGC 1 cut(s) 246
PdmI GAANNNNTTC 2 cut(s) 246, 313
PkrI GCNGC 1 cut(s) 93
PleI GAGTC 1 cut(s) 11
PpsI GAGTC 1 cut(s) 11
Psp6I CCWGG 2 cut(s) 168, 250
PspGI CCWGG 2 cut(s) 168, 250
PspN4I GGNNCC 1 cut(s) 164
PspPI GGNCC 1 cut(s) 163
RsaI GTAC 2 cut(s) 151, 256
RsaNI GTAC 2 cut(s) 150, 255
SatI GCNGC 1 cut(s) 92
Sau3AI GATC 1 cut(s) 40
Sau96I GGNCC 1 cut(s) 163
SchI GAGTC 1 cut(s) 11
ScrFI CCNGG 2 cut(s) 170, 252
SetI ASST 6 cut(s) 11, 38, 104, 158, 171, 256
SexAI ACCWGGT 1 cut(s) 168
SfaNI GCATC 1 cut(s) 42
SinI GGWCC 1 cut(s) 163
SpeI ACTAGT 1 cut(s) 331
Sse9I AATT 2 cut(s) 313, 340
SsiI CCGC 2 cut(s) 92, 301
SspMI CTAG 1 cut(s) 332
StyD4I CCNGG 2 cut(s) 168, 250
TaiI ACGT 2 cut(s) 11, 38
TasI AATT 2 cut(s) 313, 340
TatI WGTACW 1 cut(s) 149
TauI GCSGC 1 cut(s) 94
TspDTI ATGAA 4 cut(s) 17, 74, 209, 246
VpaK11BI GGWCC 1 cut(s) 163
XmnI GAANNNNTTC 2 cut(s) 246, 313
XspI CTAG 1 cut(s) 332
ZraI GACGTC 2 cut(s) 9, 36
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.