RLG00000014342

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0008889
Physical Location & Seq
Reverse (-)
51725061 .. 51729144
4084 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014342

Sequence Viewer

Length: 3099 bp
ATGGCGACTCCTGGAGGCTCTTCTTCTTGCACCCATTCATGGAGTCATGATGTCTTTCTGAGTTTCAAAGGCGCGGATACACGCTACACTTTTACATGCCATTTGCGCGAGTATTTGATTCAGAAGGGGATCAAGACCTTCATAGATGACAATGAGCTCAACAGAGGAGAGGAAATAACTCCAGCTCTTCTCAAAGCAATTGAAGAATCAAGGATTTCTGTCATAGTGTTTTCTAAAAATTATGCATTCTCGGAGTGGTGCCTGGATGAACTCACACATATCCTCTACTGTCGGAAGTCAAAGCAACAAACAGTATACCCTGTGTTTTACAAAGTAGATCCGTCTGATGTTCAGGACCAAAAAGGTAGTTTTGGTCAGGCACTTGCTCACCATGAAGACAGATTCAAGGATGACTTGGCTAAGGTGGCCAAATGGAAGGGAGCTCTTATAGAGGCCTCAAATTTGGCTGGATGGGAGTACCAGGATGGGTATGAGTCAAAATTCATTGCTACCATTGTTGAGAAGATTTCTATGCAAGTGTATTGTCATGCCGATTTTATAGTTGCACATTGCCCGGTTGGAATAGACTGTCAGCTAGAGAAAATGCATAATCTCTTAGGTGATGGGTTAAATGATGTTTGTATGATAGGGATATGGGGGATTGCTGGAATAGGTAAGACAACAATTGCCAAGGCTGTCTATAAGTCTCTTGCCTGTAAGTTTGATGGTTGCTGTTTCCTAGAAAATGTTGGAAAAGTATCAATGCAAGTTGGTGGTCTAGTCAAACTACGAAATATGCTTCTGTCGAAGATCCTAAGGGTGAAAGAATCCGATCTGCTGACTGATGCCTGTGAAGGAATAAATGTGATAGAGAACAGGTTAAGCAGAAAAAGAGTTCTATTAATTCTGGATGCTGTGGATCAATTGGAACAGTTAAGGTATTTAGTTGGAAAGTCTGAGTGGTTTGGTTTGGGCAGTAGAATTATCATAACAACAAGAGATAAACGCATATTAGCTGCTCATGGTGTCGGAGAGAACCAAATATACAAGCCGAAGGAGTTAGATGAACATGAAGCTTTAGAGCTCTTCCGGGCAAATGCCTTTCCACAAGAATGCCTCCCGGATGGTTATGCACAACTCGAAAAGGATGTATTAGAATATGCTCAAGGTCTTCCATTAGCTCTGCAACACTTGGGTAAGTTATTATATGGTAGAACTGTAGAGGAATGGAAGGAGGAATTACATAGTCATAGGAAATCTCTTCAGGCTGGTCTTCATGAGAATCTCATAATAAGTTATAATGACTTGGAGCCTTCGGTGCAGCAAGTTTTCCTTGACATTGCATGTTTCTTTAAAGGTAAACATAAGAATCATGTGATACAAATACTAGAAAAAGGTGTCGTAAAGCCTATGAATAGTATCAAAATTCTCCAAGAAAAGGCCGTCATACATATTCAAGAAGATCATATTTGTATGCACAACCTGCTAGAGGTACTGGGTAAGGAAATAGTTCACGCTGAATCATCAGAGCCTGGAAAACGAAGCAGATTATGGTTTTCAGAGGATGTTTACAAGATTCTATCAGAAGGCAAAGGAACAAAAAACATCAGAGGTGTCATAGTGTTTCCCAGTCCACATGAGATACCATTGAGTGATACATCCTTCTCAGGCATGACAAATCTCAAATATTTTATCAACAGTGATGCTGAGTTTTCTGGAGACATTGCTTGTCTCCCAAACGAGTTGAGGCTTATTGATTGGCCAAGATTTCCATTTAAATGTTTGCCACTCAATTATGTTCCCAAGAAGCTTGTTAAGCTCAATATGCCTGACAGCCTCATTTCACAACTAGGGGAGAAATTCAAGATATCAAGAACTTTGAAATATATAAATCTGGAGAACTGCCAGTTACTTAAAGAAATCCCCAACTTCTCTGGATTCCCAAATTTAAGGGACTTGAATCTGAACTACTGTACAAGTTTAGTTAAGGTTGATGATTCTGTTGGAGCCCTACATGACCTTGTTGCTTTGAGTCTTTCTGGATGCCACAACCTTGTTACCTTTCCAACAAGAATTGCCTTGAAATCTGTGAAACACATAATTTTCAGAGACTGCAGAAGTGTCAGGGCTTTGCCTGTAATTGTTCAAAAGATGGAATTCATAACATGGTTGGACCTATCTGGTACTGGTATCGAAGAGTTGCCTAATGCATCAATTAGGCACCTCATAAGCCTTGAAGAGTTAATTCTAAGGGAATGTGAATACTTAACAAGTCTGCCATGCAGCATTTATGAGTTGCACCATCTGCGGTACCTTGATCTCCACAACTGCTCAAAACTTGTGGCTCTCCCAGAGTGGAATGCAGAAATGCTTCAGACTGACTTGAATTCTTCACATAAGTTGCGGGATCTTAACCTAGGAGGTTGCAAGCAGCTTCTAAAAATTCCTAAACTTCCACCAACTGTGGAATGGGTAAATGCAGCTGATTGTGTTCAATTGGAAAGATTTTCAAAACTGTCAAACATCATGGAACATAAAGAGTCACAAATGATCAAACGTGTCACAATATCTAACTGTCAGACACTGTGTGACAATTTGGTTCGTGACATGGCAGAGACTGAAAATATTCTACCATGTCAGGCCAGTCTATACTCGCTCTTCCTCTCTTGTAGGCAGTCTGAATTTGAAGTTGTATTTCCTGGAAGTGAAGTTCCAAAGTGGTTCAGTCATAGCAAGAAGTTGAAGGAGCTGACTGAAGAATCGAAATTTTCTTTTGAAATACCTCCAAATTTCAGATTGCGGAACCAAGGGTTGGCTATCTGTGCTGCAGCTGAAGTGGTTGAGATAATTAACCCAGAAGGACAAAATAATTGCTCTGTCACAGCCACAATCGACATCAATGCAGGAAGCAAAATAGCACGTTCGTTTCATTTTGAAGGGAAAGTCATGGAATCGGGTCATGTATGGCTGTGCTACATTCCTTTCATTATTTTTACATGTTTCCTATCACCACCTTTTACTTGTGGAGTTACTTTGGAACATAAAAGCCAAGGTTCCGTGCACTGCATAAGTTATGGGGTACACCTGGTAATGTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1033

Amino Acids

116.76

Weight (kDa)

6.4

Isoelectric Point (pI)

42.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 16 - 178 6.1e-54 TIR domain
TIR_2 PF13676 18 - 114 6.7e-15 TIR domain
NB-ARC PF00931 200 - 371 2.4e-16 NB-ARC domain
WHD_ROQ1 PF23282 441 - 508 4.9e-12 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 639 - 893 2.3e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1299
Acc36I ACCTGC 1 cut(s) 1491
Acc65I GGTACC 1 cut(s) 2310
AccB1I GGYRCC 3 cut(s) 258, 2220, 2310
AccB7I CCANNNNNTGG 1 cut(s) 2812
AccI GTMKAC 1 cut(s) 315
AccII CGCG 2 cut(s) 74, 108
AciI CCGC 4 cut(s) 74, 2308, 2404, 2800
AclWI GGATC 5 cut(s) 137, 332, 805, 927, 2415
AcoI YGGCCR 2 cut(s) 426, 1760
AcuI CTGAAG 4 cut(s) 1247, 2357, 2775, 2853
AdeI CACNNNGTG 1 cut(s) 2588
AfaI GTAC 6 cut(s) 479, 1494, 1975, 2185, 2312, 3081
AfiI CCNNNNNNNGG 4 cut(s) 39, 1438, 1489, 2812
AflIII ACRYGT 2 cut(s) 2557, 2996
AjnI CCWGG 6 cut(s) 10, 261, 480, 1531, 2698, 3084
AjuI GAANNNNNNNTTGG 2 cut(s) 2795, 2827
AloI GAACNNNNNNTCC 2 cut(s) 2694, 2726
Alw21I GWGCWC 4 cut(s) 159, 445, 1086, 3063
Alw26I GTCTC 5 cut(s) 711, 1713, 1736, 2103, 2609
Alw44I GTGCAC 1 cut(s) 3059
AlwI GGATC 5 cut(s) 137, 332, 805, 927, 2415
AlwNI CAGNNNCTG 4 cut(s) 1532, 2112, 2584, 2618
AoxI GGCC 5 cut(s) 426, 453, 1440, 1760, 2640
ApaLI GTGCAC 1 cut(s) 3059
ApeKI GCWGC 7 cut(s) 1016, 1321, 2283, 2431, 2480, 2825, 2828
AseI ATTAAT 1 cut(s) 902
Asp700I GAANNNNTTC 3 cut(s) 1509, 2370, 2625
Asp718I GGTACC 1 cut(s) 2310
AspA2I CCTAGG 1 cut(s) 2416
AspLEI GCGC 2 cut(s) 74, 108
AspS9I GGNCC 2 cut(s) 355, 2173
AsuC2I CCSGG 3 cut(s) 575, 1091, 1121
AsuHPI GGTGA 4 cut(s) 380, 632, 832, 3000
AvaII GGWCC 2 cut(s) 355, 2173
AvrII CCTAGG 1 cut(s) 2416
AxyI CCTNAGG 1 cut(s) 815
BaeGI GKGCMC 1 cut(s) 3063
BalI TGGCCA 2 cut(s) 428, 1762
BanI GGYRCC 3 cut(s) 258, 2220, 2310
BanII GRGCYC 4 cut(s) 159, 445, 1086, 2011
BbsI GAAGAC 3 cut(s) 402, 1163, 1265
Bbv12I GWGCWC 4 cut(s) 159, 445, 1086, 3063
BbvI GCAGC 7 cut(s) 1003, 1333, 2295, 2443, 2492, 2812, 2840
BccI CCATC 7 cut(s) 465, 479, 617, 719, 1118, 2146, 2310
BceAI ACGGC 1 cut(s) 1427
BcgI CGANNNNNNTGC 2 cut(s) 2882, 2916
BciT130I CCWGG 6 cut(s) 12, 263, 482, 1533, 2700, 3086
BciVI GTATCC 1 cut(s) 70
BclI TGATCA 1 cut(s) 2550
BcnI CCSGG 3 cut(s) 575, 1091, 1121
BcoDI GTCTC 5 cut(s) 711, 1713, 1736, 2103, 2609
BfaI CTAG 7 cut(s) 596, 740, 779, 1388, 1487, 1850, 2417
BfmI CTRYAG 3 cut(s) 1218, 2113, 2826
BfuAI ACCTGC 1 cut(s) 1491
BfuI GTATCC 1 cut(s) 70
BisI GCNGC 7 cut(s) 1017, 1322, 2284, 2432, 2481, 2826, 2829
BlnI CCTAGG 1 cut(s) 2416
BlsI GCNGC 7 cut(s) 1018, 1323, 2285, 2433, 2482, 2827, 2830
Bme1390I CCNGG 9 cut(s) 12, 263, 482, 575, 1091, 1121, 1533, 2700, 3086
Bme18I GGWCC 2 cut(s) 355, 2173
BmgT120I GGNCC 2 cut(s) 355, 2173
BmiI GGNNCC 7 cut(s) 260, 1311, 2008, 2222, 2312, 2804, 3055
BmrFI CCNGG 9 cut(s) 12, 263, 482, 575, 1091, 1121, 1533, 2700, 3086
BmrI ACTGGG 2 cut(s) 1505, 1623
BmsI GCATC 5 cut(s) 835, 901, 1693, 2033, 2219
BmuI ACTGGG 2 cut(s) 1505, 1623
BpiI GAAGAC 3 cut(s) 402, 1163, 1265
BpmI CTGGAG 4 cut(s) 33, 165, 1737, 1916
Bpu10I CCTNAGC 1 cut(s) 420
BpuEI CTTGAG 1 cut(s) 1149
BpuMI CCSGG 3 cut(s) 575, 1091, 1121
BsaJI CCNNGG 4 cut(s) 690, 2416, 2806, 3049
Bsc4I CCNNNNNNNGG 4 cut(s) 39, 1438, 1489, 2812
Bse1I ACTGG 5 cut(s) 1500, 1629, 1906, 2191, 2643
Bse21I CCTNAGG 1 cut(s) 815
Bse3DI GCAATG 4 cut(s) 504, 568, 1338, 1722
BseBI CCWGG 6 cut(s) 12, 263, 482, 1533, 2700, 3086
BseDI CCNNGG 4 cut(s) 690, 2416, 2806, 3049
BseLI CCNNNNNNNGG 4 cut(s) 39, 1438, 1489, 2812
BseMI GCAATG 4 cut(s) 504, 568, 1338, 1722
BseMII CTCAG 4 cut(s) 50, 948, 1680, 1698
BseNI ACTGG 5 cut(s) 1500, 1629, 1906, 2191, 2643
BseRI GAGGAG 1 cut(s) 180
BseSI GKGCMC 1 cut(s) 3063
BseXI GCAGC 7 cut(s) 1003, 1333, 2295, 2443, 2492, 2812, 2840
BsgI GTGCAG 1 cut(s) 1340
Bsh1236I CGCG 2 cut(s) 74, 108
BshFI GGCC 5 cut(s) 428, 455, 1442, 1762, 2642
BshNI GGYRCC 3 cut(s) 258, 2220, 2310
BsiHKAI GWGCWC 4 cut(s) 159, 445, 1086, 3063
BsiSI CCGG 3 cut(s) 575, 1090, 1121
BslFI GGGAC 1 cut(s) 1967
BslI CCNNNNNNNGG 4 cut(s) 39, 1438, 1489, 2812
BsmAI GTCTC 5 cut(s) 711, 1713, 1736, 2103, 2609
BsmFI GGGAC 1 cut(s) 1967
BsmI GAATGC 3 cut(s) 245, 1118, 2365
BsnI GGCC 5 cut(s) 428, 455, 1442, 1762, 2642
Bsp1286I GDGCHC 5 cut(s) 159, 445, 1086, 2011, 3063
Bsp1407I TGTACA 1 cut(s) 1973
Bsp143I GATC 9 cut(s) 129, 337, 810, 832, 919, 1462, 2317, 2407, 2550
BspACI CCGC 4 cut(s) 74, 2308, 2404, 2800
BspANI GGCC 5 cut(s) 428, 455, 1442, 1762, 2642
BspCNI CTCAG 4 cut(s) 51, 949, 1679, 1699
BspFNI CGCG 2 cut(s) 74, 108
BspHI TCATGA 3 cut(s) 46, 1276, 3095
BspLI GGNNCC 7 cut(s) 260, 1311, 2008, 2222, 2312, 2804, 3055
BspMAI CTGCAG 2 cut(s) 2117, 2830
BspMI ACCTGC 1 cut(s) 1491
BspPI GGATC 5 cut(s) 137, 332, 805, 927, 2415
BspQI GCTCTTC 4 cut(s) 25, 192, 1091, 2663
BspT107I GGYRCC 3 cut(s) 258, 2220, 2310
BsrDI GCAATG 4 cut(s) 504, 568, 1338, 1722
BsrGI TGTACA 1 cut(s) 1973
BsrI ACTGG 5 cut(s) 1500, 1629, 1906, 2191, 2643
BssECI CCNNGG 4 cut(s) 690, 2416, 2806, 3049
BssMI GATC 9 cut(s) 129, 337, 810, 832, 919, 1462, 2317, 2407, 2550
BssNAI GTATAC 1 cut(s) 316
BssT1I CCWWGG 4 cut(s) 690, 2416, 2806, 3049
Bst1107I GTATAC 1 cut(s) 316
Bst2UI CCWGG 6 cut(s) 12, 263, 482, 1533, 2700, 3086
Bst6I CTCTTC 7 cut(s) 25, 192, 1091, 1266, 2190, 2232, 2663
BstAPI GCANNNNNTGC 2 cut(s) 1483, 2305
BstAUI TGTACA 1 cut(s) 1973
BstC8I GCNNGC 1 cut(s) 2429
BstDEI CTNAG 8 cut(s) 59, 420, 616, 815, 957, 1666, 1707, 2249
BstENI CCTNNNNNAGG 1 cut(s) 1487
BstFNI CGCG 2 cut(s) 74, 108
BstHHI GCGC 2 cut(s) 74, 108
BstKTI GATC 9 cut(s) 132, 340, 813, 835, 922, 1465, 2320, 2410, 2553
BstMAI GTCTC 5 cut(s) 711, 1713, 1736, 2103, 2609
BstMBI GATC 9 cut(s) 129, 337, 810, 832, 919, 1462, 2317, 2407, 2550
BstMWI GCNNNNNNNGC 8 cut(s) 105, 425, 1318, 1483, 1816, 1825, 2305, 2822
BstNI CCWGG 6 cut(s) 12, 263, 482, 1533, 2700, 3086
BstNSI RCATGY 3 cut(s) 99, 1347, 3000
BstSCI CCNGG 9 cut(s) 10, 261, 480, 573, 1089, 1119, 1531, 2698, 3084
BstSFI CTRYAG 3 cut(s) 1218, 2113, 2826
BstSLI GKGCMC 1 cut(s) 3063
BstUI CGCG 2 cut(s) 74, 108
BstV1I GCAGC 7 cut(s) 1003, 1333, 2295, 2443, 2492, 2812, 2840
BstV2I GAAGAC 3 cut(s) 402, 1163, 1265
BstX2I RGATCY 3 cut(s) 337, 810, 2407
BstYI RGATCY 3 cut(s) 337, 810, 2407
BstZ17I GTATAC 1 cut(s) 316
Bsu36I CCTNAGG 1 cut(s) 815
BsuI GTATCC 1 cut(s) 70
BsuRI GGCC 5 cut(s) 428, 455, 1442, 1762, 2642
BtsI GCAGTG 1 cut(s) 3061
BtsIMutI CAGTG 3 cut(s) 1705, 2582, 3061
BveI ACCTGC 1 cut(s) 1491
Cac8I GCNNGC 1 cut(s) 2429
CaiI CAGNNNCTG 4 cut(s) 1532, 2112, 2584, 2618
CciI TCATGA 3 cut(s) 46, 1276, 3095
CfoI GCGC 2 cut(s) 74, 108
Cfr13I GGNCC 2 cut(s) 355, 2173
CsiI ACCWGGT 1 cut(s) 3084
Csp6I GTAC 6 cut(s) 478, 1493, 1974, 2184, 2311, 3080
CspCI CAANNNNNGTGG 2 cut(s) 2322, 2357
CviQI GTAC 6 cut(s) 478, 1493, 1974, 2184, 2311, 3080
DdeI CTNAG 8 cut(s) 59, 420, 616, 815, 957, 1666, 1707, 2249
DpnI GATC 9 cut(s) 131, 339, 812, 834, 921, 1464, 2319, 2409, 2552
DpnII GATC 9 cut(s) 129, 337, 810, 832, 919, 1462, 2317, 2407, 2550
DraI TTTAAA 2 cut(s) 1354, 1777
DraIII CACNNNGTG 1 cut(s) 2588
EaeI YGGCCR 2 cut(s) 426, 1760
Eam1104I CTCTTC 7 cut(s) 25, 192, 1091, 1266, 2190, 2232, 2663
EarI CTCTTC 7 cut(s) 25, 192, 1091, 1266, 2190, 2232, 2663
Ecl136II GAGCTC 3 cut(s) 157, 443, 1084
Eco130I CCWWGG 4 cut(s) 690, 2416, 2806, 3049
Eco147I AGGCCT 1 cut(s) 455
Eco24I GRGCYC 4 cut(s) 159, 445, 1086, 2011
Eco32I GATATC 1 cut(s) 1869
Eco47I GGWCC 2 cut(s) 355, 2173
Eco53kI GAGCTC 3 cut(s) 157, 443, 1084
Eco57I CTGAAG 4 cut(s) 1247, 2357, 2775, 2853
Eco81I CCTNAGG 1 cut(s) 815
EcoICRI GAGCTC 3 cut(s) 157, 443, 1084
EcoNI CCTNNNNNAGG 1 cut(s) 1487
EcoRI GAATTC 2 cut(s) 2156, 2386
EcoRII CCWGG 6 cut(s) 10, 261, 480, 1531, 2698, 3084
EcoRV GATATC 1 cut(s) 1869
EcoT14I CCWWGG 4 cut(s) 690, 2416, 2806, 3049
EcoT22I ATGCAT 3 cut(s) 247, 609, 2212
EcoT38I GRGCYC 4 cut(s) 159, 445, 1086, 2011
ErhI CCWWGG 4 cut(s) 690, 2416, 2806, 3049
FalI AAGNNNNNCTT 4 cut(s) 398, 430, 1317, 1349
FaqI GGGAC 1 cut(s) 1967
FauI CCCGC 1 cut(s) 2397
FbaI TGATCA 1 cut(s) 2550
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 7 cut(s) 1017, 1322, 2284, 2432, 2481, 2826, 2829
FriOI GRGCYC 4 cut(s) 159, 445, 1086, 2011
Fsp4HI GCNGC 7 cut(s) 1017, 1322, 2284, 2432, 2481, 2826, 2829
FspBI CTAG 7 cut(s) 596, 740, 779, 1388, 1487, 1850, 2417
GlaI GCGC 2 cut(s) 73, 107
GluI GCNGC 7 cut(s) 1017, 1322, 2284, 2432, 2481, 2826, 2829
GsuI CTGGAG 4 cut(s) 33, 165, 1737, 1916
HaeIII GGCC 5 cut(s) 428, 455, 1442, 1762, 2642
HapII CCGG 3 cut(s) 575, 1090, 1121
HhaI GCGC 2 cut(s) 74, 108
Hin6I GCGC 2 cut(s) 72, 106
HinP1I GCGC 2 cut(s) 72, 106
HindIII AAGCTT 2 cut(s) 1074, 1808
HpaII CCGG 3 cut(s) 575, 1090, 1121
HphI GGTGA 4 cut(s) 380, 632, 832, 3000
Hpy166II GTNNAC 7 cut(s) 316, 1361, 1513, 1570, 1634, 3061, 3082
Hpy8I GTNNAC 7 cut(s) 316, 1361, 1513, 1570, 1634, 3061, 3082
HpyCH4IV ACGT 2 cut(s) 2557, 2920
HpyF10VI GCNNNNNNNGC 8 cut(s) 105, 425, 1318, 1483, 1816, 1825, 2305, 2822
HpyF3I CTNAG 8 cut(s) 59, 420, 616, 815, 957, 1666, 1707, 2249
HpySE526I ACGT 2 cut(s) 2557, 2920
HspAI GCGC 2 cut(s) 72, 106
KpnI GGTACC 1 cut(s) 2314
Ksp22I TGATCA 1 cut(s) 2550
Kzo9I GATC 9 cut(s) 129, 337, 810, 832, 919, 1462, 2317, 2407, 2550
LguI GCTCTTC 4 cut(s) 25, 192, 1091, 2663
LmnI GCTCC 4 cut(s) 440, 1309, 2006, 2746
Lsp1109I GCAGC 7 cut(s) 1003, 1333, 2295, 2443, 2492, 2812, 2840
LweI GCATC 5 cut(s) 835, 901, 1693, 2033, 2219
MabI ACCWGGT 1 cut(s) 3084
MaeI CTAG 7 cut(s) 596, 740, 779, 1388, 1487, 1850, 2417
MaeII ACGT 2 cut(s) 2557, 2920
MaeIII GTNAC 8 cut(s) 1908, 2056, 2541, 2560, 2588, 2603, 2878, 3028
MalI GATC 9 cut(s) 131, 339, 812, 834, 921, 1464, 2319, 2409, 2552
MboI GATC 9 cut(s) 129, 337, 810, 832, 919, 1462, 2317, 2407, 2550
MfeI CAATTG 4 cut(s) 198, 684, 923, 2495
MflI RGATCY 3 cut(s) 337, 810, 2407
MhlI GDGCHC 5 cut(s) 159, 445, 1086, 2011, 3063
MlsI TGGCCA 2 cut(s) 428, 1762
MluNI TGGCCA 2 cut(s) 428, 1762
MlyI GAGTC 4 cut(s) 52, 503, 2041, 2549
MmeI TCCRAC 8 cut(s) 272, 559, 730, 928, 1009, 1984, 2090, 2151
Mox20I TGGCCA 2 cut(s) 428, 1762
Mph1103I ATGCAT 3 cut(s) 247, 609, 2212
MroXI GAANNNNTTC 3 cut(s) 1509, 2370, 2625
MscI TGGCCA 2 cut(s) 428, 1762
MslI CAYNNNNRTG 2 cut(s) 1111, 1777
Msp20I TGGCCA 2 cut(s) 428, 1762
MspA1I CMGCKG 2 cut(s) 2483, 2831
MspI CCGG 3 cut(s) 575, 1090, 1121
MspR9I CCNGG 9 cut(s) 12, 263, 482, 575, 1091, 1121, 1533, 2700, 3086
MunI CAATTG 4 cut(s) 198, 684, 923, 2495
Mva1269I GAATGC 3 cut(s) 245, 1118, 2365
MvaI CCWGG 6 cut(s) 12, 263, 482, 1533, 2700, 3086
MvnI CGCG 2 cut(s) 74, 108
MwoI GCNNNNNNNGC 8 cut(s) 105, 425, 1318, 1483, 1816, 1825, 2305, 2822
NciI CCSGG 3 cut(s) 575, 1091, 1121
NdeII GATC 9 cut(s) 129, 337, 810, 832, 919, 1462, 2317, 2407, 2550
NlaIV GGNNCC 7 cut(s) 260, 1311, 2008, 2222, 2312, 2804, 3055
NmuCI GTSAC 5 cut(s) 2541, 2560, 2588, 2603, 2878
NsiI ATGCAT 3 cut(s) 247, 609, 2212
NspI RCATGY 3 cut(s) 99, 1347, 3000
PagI TCATGA 3 cut(s) 46, 1276, 3095
PceI AGGCCT 1 cut(s) 455
PciI ACATGT 1 cut(s) 2996
PciSI GCTCTTC 4 cut(s) 25, 192, 1091, 2663
PctI GAATGC 3 cut(s) 245, 1118, 2365
PdmI GAANNNNTTC 3 cut(s) 1509, 2370, 2625
PflMI CCANNNNNTGG 1 cut(s) 2812
PfoI TCCNGGA 3 cut(s) 10, 1119, 2698
PkrI GCNGC 7 cut(s) 1018, 1323, 2285, 2433, 2482, 2827, 2830
PleI GAGTC 4 cut(s) 51, 502, 2040, 2548
PpsI GAGTC 4 cut(s) 51, 502, 2040, 2548
PscI ACATGT 1 cut(s) 2996
PshBI ATTAAT 1 cut(s) 902
PsiI TTATAA 1 cut(s) 1299
Psp124BI GAGCTC 3 cut(s) 159, 445, 1086
Psp6I CCWGG 6 cut(s) 10, 261, 480, 1531, 2698, 3084
PspGI CCWGG 6 cut(s) 10, 261, 480, 1531, 2698, 3084
PspN4I GGNNCC 7 cut(s) 260, 1311, 2008, 2222, 2312, 2804, 3055
PspPI GGNCC 2 cut(s) 355, 2173
PsrI GAACNNNNNNTAC 2 cut(s) 1028, 1060
PstI CTGCAG 2 cut(s) 2117, 2830
PstNI CAGNNNCTG 4 cut(s) 1532, 2112, 2584, 2618
PsuI RGATCY 3 cut(s) 337, 810, 2407
PvuII CAGCTG 2 cut(s) 2483, 2831
RsaI GTAC 6 cut(s) 479, 1494, 1975, 2185, 2312, 3081
RsaNI GTAC 6 cut(s) 478, 1493, 1974, 2184, 2311, 3080
RseI CAYNNNNRTG 2 cut(s) 1111, 1777
SacI GAGCTC 3 cut(s) 159, 445, 1086
SapI GCTCTTC 4 cut(s) 25, 192, 1091, 2663
SatI GCNGC 7 cut(s) 1017, 1322, 2284, 2432, 2481, 2826, 2829
Sau3AI GATC 9 cut(s) 129, 337, 810, 832, 919, 1462, 2317, 2407, 2550
Sau96I GGNCC 2 cut(s) 355, 2173
SchI GAGTC 4 cut(s) 52, 503, 2041, 2549
ScrFI CCNGG 9 cut(s) 12, 263, 482, 575, 1091, 1121, 1533, 2700, 3086
SduI GDGCHC 5 cut(s) 159, 445, 1086, 2011, 3063
SexAI ACCWGGT 1 cut(s) 3084
SfaNI GCATC 5 cut(s) 835, 901, 1693, 2033, 2219
SfcI CTRYAG 3 cut(s) 1218, 2113, 2826
SinI GGWCC 2 cut(s) 355, 2173
SmiI ATTTAAAT 1 cut(s) 1777
SmiMI CAYNNNNRTG 2 cut(s) 1111, 1777
SmlI CTYRAG 1 cut(s) 1164
SmoI CTYRAG 1 cut(s) 1164
SseBI AGGCCT 1 cut(s) 455
SsiI CCGC 4 cut(s) 74, 2308, 2404, 2800
SspI AATATT 2 cut(s) 1688, 2626
SspMI CTAG 7 cut(s) 596, 740, 779, 1388, 1487, 1850, 2417
SstI GAGCTC 3 cut(s) 159, 445, 1086
StuI AGGCCT 1 cut(s) 455
StyD4I CCNGG 9 cut(s) 10, 261, 480, 573, 1089, 1119, 1531, 2698, 3084
StyI CCWWGG 4 cut(s) 690, 2416, 2806, 3049
SwaI ATTTAAAT 1 cut(s) 1777
TaiI ACGT 2 cut(s) 2560, 2923
TaqI TCGA 5 cut(s) 806, 1140, 2193, 2762, 2892
TatI WGTACW 1 cut(s) 1973
TscAI CASTG 3 cut(s) 1705, 2589, 3068
TseFI GTSAC 5 cut(s) 2541, 2560, 2588, 2603, 2878
TseI GCWGC 7 cut(s) 1016, 1321, 2283, 2431, 2480, 2825, 2828
Tsp45I GTSAC 5 cut(s) 2541, 2560, 2588, 2603, 2878
TspGWI ACGGA 2 cut(s) 330, 3046
TspRI CASTG 3 cut(s) 1705, 2589, 3068
Van91I CCANNNNNTGG 1 cut(s) 2812
VneI GTGCAC 1 cut(s) 3059
VpaK11BI GGWCC 2 cut(s) 355, 2173
VspI ATTAAT 1 cut(s) 902
XagI CCTNNNNNAGG 1 cut(s) 1487
XceI RCATGY 3 cut(s) 99, 1347, 3000
XcmI CCANNNNNNNNNTGG 1 cut(s) 2466
XmaJI CCTAGG 1 cut(s) 2416
XmiI GTMKAC 1 cut(s) 315
XmnI GAANNNNTTC 3 cut(s) 1509, 2370, 2625
XspI CTAG 7 cut(s) 596, 740, 779, 1388, 1487, 1850, 2417
Zsp2I ATGCAT 3 cut(s) 247, 609, 2212
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.