Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0008889

Physical Location & Seq

Genomic Coordinates Reverse (-)

48566177 .. 48570122

Gene Structure 3946 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00201070.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2628 bp

ATGACCCCTCAACCTGGACCTCCATCCTCCTCATCTTCTTCTTGCTCTTCTTCTTTGTCTTCCACAGATAACCATGAGAGCAAACACGATGTCTTTTTGAGTTTTAGAGGCGAGGACACACGCGATAATTTTACAGACCATTTGTACAGCGCTCTGTGTCGAAAGGGAATTAAAACCTTCATAGACAATAAACTTGACAGGGGAGAAGAAATATCAGACGCACTTCTAAAAGCAATTGAAGAGTCAAGGATCTCTATTGTCGTATTCTCTAAAACTTATGCCTCTTCGAAGTGGTGTTTGGATGAACTCGTGAAGATTCTTCAATGTAAAAAGTCAAAGAATCAGATGGTTAGACCCGTTTTCTACAAGGTGGATCCCTCAAATGTACGGAACCAAAGTGGTAGTTTTGGTGAAGCGCTTGCTCAACATGAGAGGAGATTCAACAATAAAATGGAGAGGGTCTTGTCGTGGATAAGGAATTTGCTGAACTGGTCTTTGAGTTATGATGACAGCGAATTCAAGCATAACATGAAGAAGCTGAATAAATGGACCAAAGCTCTCAAGGAAGCGGCGGACTTGTCTGGATGGACTTACTTGAATGGGTCTGAATCAAAATTTATCGATAACATTGTTGAAGAAATTTCAGAACAAGTTTTACGTCATAGCAATTTTAATGTGGCGGATCATCCAGTTGGAATACATTCTCGAGTACGAGATATGCACAATGTTTTAGGTCTTGGGGTGAAAGATGTTCGAATGGTAGGAATATGGGGTCATTTTGAAACTCTCCGAATAAGTTACAATAACTTGGAAGATTCGGTAAAGAATGTTTTCCTTGACATTGCATTTTTCTTCAACGGTCAAGATGAGAACCATGTGTTACAGATCCTAGAAGGTGGTGACAAGCCTAACAAAAGCATTAGTGAGCTCAAAGAAAAGGCACTCATAAGGATTAACGAAAAAAATCATATCTTGATGCACAATATACTAGAAGTAATGGCTAAAGAGATAGTTCACATTGAGTCACCAAAGAACCCCGCTAGACGTAGCCGATTGTGGTTTTCTGATGATGTTTACGATGTTCTCACAAAATGCACGGGAACAGACAATATCAAAGGCGTCATGGTAATGTTTGGCGGACCACGTGAGATAGCACTGCAAAATAACAGCTTCGATGGGATGACAAATCTTCAGTTCTTTATAAACACCGATGCACACTTTTCTGGAGACATTGAATATCTCCCCGACAATTTAAGACTTATTGATTGGCCTGAATTTCCCTTTGGATCATTTCCAATCAATTTTAATCCAACTAAGCTTGTTAAGCTTAGTATACCTCATAGCCTCATCTCCCAACTGGGAGAGACATTCAAGAGTTGCAGTAACCTGAAATATATAAACTTGGAAAGCTGCCACTTCCTTACTAGAATTCCCGACTTGTCAGGATTCCCAAATTTAAAGGAGCTGAATCTTAACAATTGTACAAGCTTAGTTGAGGTTGATCAGTCTGTTGGACTTCTTGATAAGCTTGTTATTTTGAGTCTTTCTGGATGTGACAGGCTCACTAGATTGCTTAAGACATTCTGCGACATTCTGGAAAATGTGGTATCCTTGACAAGGCTGGATCTATCTGGCACGGCCATTGAAGAATTGCCTGAAACAGTTGTATATCTCGTCAGCCTTAAACATTTAATTCTAAGACAATGTGATAAGCTTATCCATCTGCCACATAGCATTTATGATCTGCATAGTTTATCACGTCTTGACCTGCAGAACTGCTCAAAGCTTGTTACGCTTCCAAGGTGGAATGATGAGCAGTCAGTTTCGACTACCTTGAGGTTTCTTGACCTGCGTGGTTGCACAGAGCTTCCAGAAATTCCCGAACTTCCACCGAGCGTGGAATGGATAAATGCTGATGGCTGTGAGTCATTGGTCCGAGTTGCAAAACTTTCAAACATCTTGGAACGTAGAGCTTCGCAAATGATTGAATCAATTTCATTGTATAATTGTCAGAGATTGTGTGACAATCTACCTCATTGCAGGGCAGGTAATAAGAGTAGTCTCTTCTCTCTCATTCACTTGGCTAAGCAGTCTGAATTCGAGATTGTATTTCCAGCAAGTGAAGTTCCTAGTTGGTTCAGGAATCGCGAGTATTTAATGGAGCTTTTTGATGAACGTGAATTTTCTTTTGAAATTCCTCTAAATTTCAACAAAGGATTGGCTATCTGTGCTTCAGCAACTGCTGTGGGTAATCTTGATCAGTGCTCTTTCACAGCCAGTATTCACATCAATGGAGAAAGCAGGTGTACTTATTCATTTTGTTTCGAAGCAAGCAAGATGAAGGACTCTGCTCATGTATGGCTGCTCTATATTCCATTTGTTAAGTTTGCGTTTCATGAGCCACCTTATAGCTGCCTAGTTAATTTGAAACATACTAGTAAAGATTCGGTGCGCTGTAGAAGCTATGGGGTTCACCTGGTAATGCTACAGGATGAATATGAAGATTTCGTAGATGACAGCGACTTGGAAGATGAGTACTCAGCTGGTGAAAATGATGAAGACATGATAGATCAAATTGAGGATGGTGAAGACTTGGAAAATATCAGACCCAAAAAGAGAAGGCGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NB-ARC TIR

Protein Analysis

875

Amino Acids

100.01

Weight (kDa)

5.62

Isoelectric Point (pI)

53.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIR	PF01582	28 - 163	4.5e-51	TIR domain
TIR_2	PF13676	31 - 126	1.6e-15	TIR domain
WHD_ROQ1	PF23282	275 - 339	2.7e-08	Disease resistance protein Roq1-like, winged-helix domain
LRR_14	PF23598	476 - 616	2.7e-07	Leucine-rich repeat region

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008889)

Species	Orthologous Gene IDs
prunus_persica	Prupe.8G110000_v2.0.a1
rosa_chinensis	RchiOBHm_Chr6g0262301 RchiOBHm_Chr6g0262321
rosa_laevigata	RLG00000014024 RLG00000014034 RLG00000014334 RLG00000014342 RLG00000035301
rosa_multiflora	Rmu_sc0000565.1_g000041 Rmu_sc0002938.1_g000003 Rmu_sc0002938.1_g000011 Rmu_ssc0000388.1_g000023
rosa_roxburghii	Rroxscaffold_7G00201070 Rroxscaffold_7G00201110 Rroxscaffold_7G00204190 Rroxscaffold_7G00204430
rosa_rugosa	Rorug06G0029600 Rorug06G0029800
rosa_samantha	Rh1AG095400 Rh1AG095600 Rh6AG151300 Rh6DG136200
rosa_wichuraiana	Rw6G013100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1202
AarI	CACCTGC	1 cut(s)	2292
Acc36I	ACCTGC	4 cut(s)	1776, 1857, 2036, 2292
AccI	GTMKAC	1 cut(s)	1333
AccII	CGCG	2 cut(s)	123, 2148
AciI	CCGC	5 cut(s)	569, 572, 680, 1038, 1137
AclWI	GGATC	7 cut(s)	257, 368, 381, 690, 880, 1294, 1632
AcoI	YGGCCR	1 cut(s)	1638
AcuI	CTGAAG	2 cut(s)	1175, 2217
AcvI	CACGTG	1 cut(s)	1145
AcyI	GRCGYC	1 cut(s)	1119
AfaI	GTAC	6 cut(s)	146, 387, 711, 1483, 2308, 2537
AfeI	AGCGCT	2 cut(s)	151, 417
AfiI	CCNNNNNNNGG	2 cut(s)	14, 1617
AflII	CTTAAG	1 cut(s)	1574
AhlI	ACTAGT	1 cut(s)	2435
AjiI	CACGTC	1 cut(s)	1760
AjnI	CCWGG	2 cut(s)	13, 2475
AjuI	GAANNNNNNNTTGG	4 cut(s)	281, 313, 1266, 1298
Alw21I	GWGCWC	2 cut(s)	930, 2267
Alw26I	GTCTC	3 cut(s)	1221, 1358, 2066
AlwI	GGATC	7 cut(s)	257, 368, 381, 690, 880, 1294, 1632
AlwNI	CAGNNNCTG	1 cut(s)	2240
Ama87I	CYCGRG	1 cut(s)	705
Aor51HI	AGCGCT	2 cut(s)	151, 417
AoxI	GGCC	2 cut(s)	1268, 1638
ApeKI	GCWGC	3 cut(s)	1410, 2362, 2412
ArsI	GACNNNNNNTTYG	2 cut(s)	75, 107
Asp700I	GAANNNNTTC	2 cut(s)	700, 830
AspLEI	GCGC	3 cut(s)	152, 418, 2454
AspS9I	GGNCC	4 cut(s)	17, 549, 1139, 1933
AsuHPI	GGTGA	7 cut(s)	422, 754, 911, 1017, 2465, 2558, 2597
AsuII	TTCGAA	3 cut(s)	287, 754, 2325
AvaI	CYCGRG	1 cut(s)	705
AvaII	GGWCC	4 cut(s)	17, 549, 1139, 1933
BamHI	GGATCC	1 cut(s)	373
BanII	GRGCYC	1 cut(s)	930
BauI	CACGAG	1 cut(s)	308
BbrPI	CACGTG	1 cut(s)	1145
BbsI	GAAGAC	3 cut(s)	51, 2565, 2595
Bbv12I	GWGCWC	2 cut(s)	930, 2267
BbvI	GCAGC	3 cut(s)	1397, 2349, 2399
BccI	CCATC	7 cut(s)	31, 340, 579, 1169, 1728, 1910, 2576
BceAI	ACGGC	1 cut(s)	1653
BciT130I	CCWGG	2 cut(s)	15, 2477
BciVI	GTATCC	1 cut(s)	1618
BclI	TGATCA	2 cut(s)	1501, 2257
BcoDI	GTCTC	3 cut(s)	1221, 1358, 2066
BcuI	ACTAGT	1 cut(s)	2435
BfaI	CTAG	8 cut(s)	890, 989, 1041, 1425, 1566, 2130, 2417, 2436
BfmI	CTRYAG	3 cut(s)	1769, 2455, 2486
BfoI	RGCGCY	2 cut(s)	153, 419
BfrI	CTTAAG	1 cut(s)	1574
BfuAI	ACCTGC	4 cut(s)	1776, 1857, 2036, 2292
BfuI	GTATCC	1 cut(s)	1618
BisI	GCNGC	4 cut(s)	570, 1411, 2363, 2413
BlpI	GCTNAGC	1 cut(s)	2085
BlsI	GCNGC	4 cut(s)	571, 1412, 2364, 2414
BmcAI	AGTACT	1 cut(s)	2537
Bme1390I	CCNGG	2 cut(s)	15, 2477
Bme18I	GGWCC	4 cut(s)	17, 549, 1139, 1933
BmeT110I	CYCGRG	1 cut(s)	705
BmgBI	CACGTC	1 cut(s)	1760
BmgT120I	GGNCC	4 cut(s)	17, 549, 1139, 1933
BmiI	GGNNCC	2 cut(s)	375, 392
BmrFI	CCNGG	2 cut(s)	15, 2477
BmrI	ACTGGG	1 cut(s)	1367
BmsI	GCATC	2 cut(s)	966, 1201
BmuI	ACTGGG	1 cut(s)	1367
BpiI	GAAGAC	3 cut(s)	51, 2565, 2595
BpmI	CTGGAG	1 cut(s)	1245
Bpu1102I	GCTNAGC	1 cut(s)	2085
Bpu14I	TTCGAA	3 cut(s)	287, 754, 2325
BpuEI	CTTGAG	2 cut(s)	545, 1855
Bsa29I	ATCGAT	1 cut(s)	621
BsaAI	YACGTR	1 cut(s)	1145
BsaHI	GRCGYC	1 cut(s)	1119
BsaJI	CCNNGG	1 cut(s)	1799
Bsc4I	CCNNNNNNNGG	2 cut(s)	14, 1617
Bse1I	ACTGG	4 cut(s)	494, 689, 1362, 2277
Bse3DI	GCAATG	2 cut(s)	840, 2035
BseBI	CCWGG	2 cut(s)	15, 2477
BseCI	ATCGAT	1 cut(s)	621
BseDI	CCNNGG	1 cut(s)	1799
BseGI	GGATG	8 cut(s)	23, 307, 590, 685, 1185, 1556, 2497, 2587
BseLI	CCNNNNNNNGG	2 cut(s)	14, 1617
BseMI	GCAATG	2 cut(s)	840, 2035
BseMII	CTCAG	1 cut(s)	2553
BseNI	ACTGG	4 cut(s)	494, 689, 1362, 2277
BseRI	GAGGAG	2 cut(s)	19, 448
BseXI	GCAGC	3 cut(s)	1397, 2349, 2399
Bsh1236I	CGCG	2 cut(s)	123, 2148
BshFI	GGCC	2 cut(s)	1270, 1640
BshVI	ATCGAT	1 cut(s)	621
BsiHKAI	GWGCWC	2 cut(s)	930, 2267
BsiHKCI	CYCGRG	1 cut(s)	705
BslI	CCNNNNNNNGG	2 cut(s)	14, 1617
BsmAI	GTCTC	3 cut(s)	1221, 1358, 2066
BsnI	GGCC	2 cut(s)	1270, 1640
BsoBI	CYCGRG	1 cut(s)	705
Bsp119I	TTCGAA	3 cut(s)	287, 754, 2325
Bsp1286I	GDGCHC	2 cut(s)	930, 2267
Bsp1407I	TGTACA	2 cut(s)	144, 1481
Bsp1720I	GCTNAGC	1 cut(s)	2085
Bsp68I	TCGCGA	1 cut(s)	2148
BspACI	CCGC	5 cut(s)	569, 572, 680, 1038, 1137
BspANI	GGCC	2 cut(s)	1270, 1640
BspCNI	CTCAG	1 cut(s)	2552
BspDI	ATCGAT	1 cut(s)	621
BspFNI	CGCG	2 cut(s)	123, 2148
BspHI	TCATGA	1 cut(s)	2395
BspLI	GGNNCC	2 cut(s)	375, 392
BspMAI	CTGCAG	1 cut(s)	1773
BspMI	ACCTGC	4 cut(s)	1776, 1857, 2036, 2292
BspPI	GGATC	7 cut(s)	257, 368, 381, 690, 880, 1294, 1632
BspQI	GCTCTTC	1 cut(s)	52
BspT104I	TTCGAA	3 cut(s)	287, 754, 2325
BspTI	CTTAAG	1 cut(s)	1574
BsrDI	GCAATG	2 cut(s)	840, 2035
BsrGI	TGTACA	2 cut(s)	144, 1481
BsrI	ACTGG	4 cut(s)	494, 689, 1362, 2277
BssECI	CCNNGG	1 cut(s)	1799
BssNAI	GTATAC	1 cut(s)	1334
BssNI	GRCGYC	1 cut(s)	1119
BssSI	CACGAG	1 cut(s)	308
BssT1I	CCWWGG	1 cut(s)	1799
Bst1107I	GTATAC	1 cut(s)	1334
Bst2BI	CACGAG	1 cut(s)	308
Bst2UI	CCWGG	2 cut(s)	15, 2477
Bst4CI	ACNGT	2 cut(s)	860, 1663
Bst6I	CTCTTC	4 cut(s)	52, 234, 289, 2069
BstACI	GRCGYC	1 cut(s)	1119
BstAFI	CTTAAG	1 cut(s)	1574
BstAUI	TGTACA	2 cut(s)	144, 1481
BstBAI	YACGTR	1 cut(s)	1145
BstBI	TTCGAA	3 cut(s)	287, 754, 2325
BstC8I	GCNNGC	2 cut(s)	420, 2332
BstDEI	CTNAG	6 cut(s)	1314, 1328, 1489, 1697, 2085, 2539
BstENI	CCTNNNNNAGG	1 cut(s)	1615
BstF5I	GGATG	8 cut(s)	23, 307, 590, 685, 1185, 1556, 2497, 2587
BstFNI	CGCG	2 cut(s)	123, 2148
BstH2I	RGCGCY	2 cut(s)	153, 419
BstHHI	GCGC	3 cut(s)	152, 418, 2454
BstMAI	GTCTC	3 cut(s)	1221, 1358, 2066
BstMWI	GCNNNNNNNGC	4 cut(s)	1324, 1792, 2228, 2460
BstNI	CCWGG	2 cut(s)	15, 2477
BstSCI	CCNGG	2 cut(s)	13, 2475
BstSFI	CTRYAG	3 cut(s)	1769, 2455, 2486
BstUI	CGCG	2 cut(s)	123, 2148
BstV1I	GCAGC	3 cut(s)	1397, 2349, 2399
BstV2I	GAAGAC	3 cut(s)	51, 2565, 2595
BstX2I	RGATCY	4 cut(s)	249, 373, 885, 1624
BstYI	RGATCY	4 cut(s)	249, 373, 885, 1624
BstZ17I	GTATAC	1 cut(s)	1334
Bsu15I	ATCGAT	1 cut(s)	621
BsuI	GTATCC	1 cut(s)	1618
BsuRI	GGCC	2 cut(s)	1270, 1640
BsuTUI	ATCGAT	1 cut(s)	621
BtrI	CACGTC	1 cut(s)	1760
BtsCI	GGATG	8 cut(s)	23, 307, 590, 685, 1185, 1556, 2497, 2587
BtsI	GCAGTG	1 cut(s)	1154
BtsIMutI	CAGTG	2 cut(s)	1154, 2267
BtuMI	TCGCGA	1 cut(s)	2148
BveI	ACCTGC	4 cut(s)	1776, 1857, 2036, 2292
Cac8I	GCNNGC	2 cut(s)	420, 2332
CaiI	CAGNNNCTG	1 cut(s)	2240
CciI	TCATGA	1 cut(s)	2395
CfoI	GCGC	3 cut(s)	152, 418, 2454
Cfr13I	GGNCC	4 cut(s)	17, 549, 1139, 1933
ClaI	ATCGAT	1 cut(s)	621
CseI	GACGC	2 cut(s)	227, 1108
CsiI	ACCWGGT	1 cut(s)	2475
Csp6I	GTAC	6 cut(s)	145, 386, 710, 1482, 2307, 2536
CspCI	CAANNNNNGTGG	2 cut(s)	2226, 2261
CviAII	CATG	8 cut(s)	74, 428, 529, 875, 1123, 2354, 2396, 2563
CviQI	GTAC	6 cut(s)	145, 386, 710, 1482, 2307, 2536
DdeI	CTNAG	6 cut(s)	1314, 1328, 1489, 1697, 2085, 2539
DraI	TTTAAA	1 cut(s)	1458
EaeI	YGGCCR	1 cut(s)	1638
Eam1104I	CTCTTC	4 cut(s)	52, 234, 289, 2069
EarI	CTCTTC	4 cut(s)	52, 234, 289, 2069
EciI	GGCGGA	3 cut(s)	587, 695, 1152
Ecl136II	GAGCTC	1 cut(s)	928
Eco130I	CCWWGG	1 cut(s)	1799
Eco24I	GRGCYC	1 cut(s)	930
Eco47I	GGWCC	4 cut(s)	17, 549, 1139, 1933
Eco47III	AGCGCT	2 cut(s)	151, 417
Eco53kI	GAGCTC	1 cut(s)	928
Eco57I	CTGAAG	2 cut(s)	1175, 2217
Eco72I	CACGTG	1 cut(s)	1145
Eco88I	CYCGRG	1 cut(s)	705
EcoICRI	GAGCTC	1 cut(s)	928
EcoNI	CCTNNNNNAGG	1 cut(s)	1615
EcoRI	GAATTC	3 cut(s)	515, 1428, 2096
EcoRII	CCWGG	2 cut(s)	13, 2475
EcoT14I	CCWWGG	1 cut(s)	1799
EcoT38I	GRGCYC	1 cut(s)	930
ErhI	CCWWGG	1 cut(s)	1799
FaeI	CATG	8 cut(s)	77, 431, 532, 878, 1126, 2357, 2399, 2566
FatI	CATG	8 cut(s)	73, 427, 528, 874, 1122, 2353, 2395, 2562
FauI	CCCGC	1 cut(s)	1045
FbaI	TGATCA	2 cut(s)	1501, 2257
FblI	GTMKAC	1 cut(s)	1333
Fnu4HI	GCNGC	4 cut(s)	570, 1411, 2363, 2413
FokI	GGATG	8 cut(s)	10, 314, 597, 672, 1192, 1563, 2504, 2594
FriOI	GRGCYC	1 cut(s)	930
Fsp4HI	GCNGC	4 cut(s)	570, 1411, 2363, 2413
FspBI	CTAG	8 cut(s)	890, 989, 1041, 1425, 1566, 2130, 2417, 2436
GlaI	GCGC	3 cut(s)	151, 417, 2453
GluI	GCNGC	4 cut(s)	570, 1411, 2363, 2413
GsuI	CTGGAG	1 cut(s)	1245
HaeII	RGCGCY	2 cut(s)	153, 419
HaeIII	GGCC	2 cut(s)	1270, 1640
HgaI	GACGC	2 cut(s)	227, 1108
HhaI	GCGC	3 cut(s)	152, 418, 2454
Hin1I	GRCGYC	1 cut(s)	1119
Hin1II	CATG	8 cut(s)	77, 431, 532, 878, 1126, 2357, 2399, 2566
Hin6I	GCGC	3 cut(s)	150, 416, 2452
HinP1I	GCGC	3 cut(s)	150, 416, 2452
HindIII	AAGCTT	6 cut(s)	1316, 1325, 1486, 1526, 1712, 1784
HphI	GGTGA	7 cut(s)	422, 754, 911, 1017, 2465, 2558, 2597
Hpy166II	GTNNAC	5 cut(s)	1015, 1075, 1334, 2307, 2473
Hpy8I	GTNNAC	5 cut(s)	1015, 1075, 1334, 2307, 2473
HpyAV	CCTTC	4 cut(s)	187, 887, 2335, 2613
HpyCH4III	ACNGT	2 cut(s)	860, 1663
HpyCH4IV	ACGT	6 cut(s)	658, 1045, 1144, 1759, 1966, 2176
HpyF10VI	GCNNNNNNNGC	4 cut(s)	1324, 1792, 2228, 2460
HpyF3I	CTNAG	6 cut(s)	1314, 1328, 1489, 1697, 2085, 2539
HpySE526I	ACGT	6 cut(s)	658, 1045, 1144, 1759, 1966, 2176
Hsp92I	GRCGYC	1 cut(s)	1119
Hsp92II	CATG	8 cut(s)	77, 431, 532, 878, 1126, 2357, 2399, 2566
HspAI	GCGC	3 cut(s)	150, 416, 2452
Ksp22I	TGATCA	2 cut(s)	1501, 2257
LguI	GCTCTTC	1 cut(s)	52
LmnI	GCTCC	2 cut(s)	1462, 2161
Lsp1109I	GCAGC	3 cut(s)	1397, 2349, 2399
LweI	GCATC	2 cut(s)	966, 1201
MabI	ACCWGGT	1 cut(s)	2475
MaeI	CTAG	8 cut(s)	890, 989, 1041, 1425, 1566, 2130, 2417, 2436
MaeII	ACGT	6 cut(s)	658, 1045, 1144, 1759, 1966, 2176
MaeIII	GTNAC	8 cut(s)	797, 879, 899, 1023, 1382, 1553, 1789, 2021
MfeI	CAATTG	2 cut(s)	234, 1477
MflI	RGATCY	4 cut(s)	249, 373, 885, 1624
MhlI	GDGCHC	2 cut(s)	930, 2267
MlyI	GAGTC	5 cut(s)	251, 1031, 1549, 1934, 2339
MmeI	TCCRAC	3 cut(s)	673, 1334, 1492
MroXI	GAANNNNTTC	2 cut(s)	700, 830
MslI	CAYNNNNRTG	2 cut(s)	1127, 2289
MspA1I	CMGCKG	1 cut(s)	2543
MspCI	CTTAAG	1 cut(s)	1574
MspR9I	CCNGG	2 cut(s)	15, 2477
MunI	CAATTG	2 cut(s)	234, 1477
MvaI	CCWGG	2 cut(s)	15, 2477
MvnI	CGCG	2 cut(s)	123, 2148
MwoI	GCNNNNNNNGC	4 cut(s)	1324, 1792, 2228, 2460
NlaIII	CATG	8 cut(s)	77, 431, 532, 878, 1126, 2357, 2399, 2566
NlaIV	GGNNCC	2 cut(s)	375, 392
NmuCI	GTSAC	4 cut(s)	899, 1023, 1553, 2021
NruI	TCGCGA	1 cut(s)	2148
NspV	TTCGAA	3 cut(s)	287, 754, 2325
PaeR7I	CTCGAG	1 cut(s)	705
PagI	TCATGA	1 cut(s)	2395
PaqCI	CACCTGC	1 cut(s)	2292
PciSI	GCTCTTC	1 cut(s)	52
PdmI	GAANNNNTTC	2 cut(s)	700, 830
PkrI	GCNGC	4 cut(s)	571, 1412, 2364, 2414
PleI	GAGTC	5 cut(s)	250, 1030, 1548, 1933, 2339
PmaCI	CACGTG	1 cut(s)	1145
PmlI	CACGTG	1 cut(s)	1145
PpsI	GAGTC	5 cut(s)	250, 1030, 1548, 1933, 2339
Ppu21I	YACGTR	1 cut(s)	1145
PsiI	TTATAA	1 cut(s)	1202
Psp124BI	GAGCTC	1 cut(s)	930
Psp6I	CCWGG	2 cut(s)	13, 2475
PspCI	CACGTG	1 cut(s)	1145
PspGI	CCWGG	2 cut(s)	13, 2475
PspN4I	GGNNCC	2 cut(s)	375, 392
PspPI	GGNCC	4 cut(s)	17, 549, 1139, 1933
PsrI	GAACNNNNNNTAC	2 cut(s)	639, 671
PstI	CTGCAG	1 cut(s)	1773
PstNI	CAGNNNCTG	1 cut(s)	2240
PsuI	RGATCY	4 cut(s)	249, 373, 885, 1624
PvuII	CAGCTG	1 cut(s)	2543
RruI	TCGCGA	1 cut(s)	2148
RsaI	GTAC	6 cut(s)	146, 387, 711, 1483, 2308, 2537
RsaNI	GTAC	6 cut(s)	145, 386, 710, 1482, 2307, 2536
RseI	CAYNNNNRTG	2 cut(s)	1127, 2289
SacI	GAGCTC	1 cut(s)	930
SapI	GCTCTTC	1 cut(s)	52
SatI	GCNGC	4 cut(s)	570, 1411, 2363, 2413
Sau96I	GGNCC	4 cut(s)	17, 549, 1139, 1933
ScaI	AGTACT	1 cut(s)	2537
SchI	GAGTC	5 cut(s)	251, 1031, 1549, 1934, 2339
ScrFI	CCNGG	2 cut(s)	15, 2477
SduI	GDGCHC	2 cut(s)	930, 2267
SexAI	ACCWGGT	1 cut(s)	2475
SfaNI	GCATC	2 cut(s)	966, 1201
SfcI	CTRYAG	3 cut(s)	1769, 2455, 2486
Sfr274I	CTCGAG	1 cut(s)	705
SfuI	TTCGAA	3 cut(s)	287, 754, 2325
SinI	GGWCC	4 cut(s)	17, 549, 1139, 1933
SlaI	CTCGAG	1 cut(s)	705
SmiMI	CAYNNNNRTG	2 cut(s)	1127, 2289
SmlI	CTYRAG	4 cut(s)	560, 705, 1574, 1834
SmoI	CTYRAG	4 cut(s)	560, 705, 1574, 1834
SpeI	ACTAGT	1 cut(s)	2435
SsiI	CCGC	5 cut(s)	569, 572, 680, 1038, 1137
SspMI	CTAG	8 cut(s)	890, 989, 1041, 1425, 1566, 2130, 2417, 2436
SstI	GAGCTC	1 cut(s)	930
StyD4I	CCNGG	2 cut(s)	13, 2475
StyI	CCWWGG	1 cut(s)	1799
TaaI	ACNGT	2 cut(s)	860, 1663
TaiI	ACGT	6 cut(s)	661, 1048, 1147, 1762, 1969, 2179
TaqI	TCGA	9 cut(s)	160, 287, 621, 706, 754, 1173, 1826, 2100, 2325
TatI	WGTACW	4 cut(s)	144, 1481, 2306, 2535
TauI	GCSGC	1 cut(s)	572
TscAI	CASTG	2 cut(s)	1161, 2267
TseFI	GTSAC	4 cut(s)	899, 1023, 1553, 2021
TseI	GCWGC	3 cut(s)	1410, 2362, 2412
Tsp45I	GTSAC	4 cut(s)	899, 1023, 1553, 2021
TspGWI	ACGGA	1 cut(s)	403
TspRI	CASTG	2 cut(s)	1161, 2267
Vha464I	CTTAAG	1 cut(s)	1574
VpaK11BI	GGWCC	4 cut(s)	17, 549, 1139, 1933
XagI	CCTNNNNNAGG	1 cut(s)	1615
XhoI	CTCGAG	1 cut(s)	705
XmiI	GTMKAC	1 cut(s)	1333
XmnI	GAANNNNTTC	2 cut(s)	700, 830
XspI	CTAG	8 cut(s)	890, 989, 1041, 1425, 1566, 2130, 2417, 2436
ZrmI	AGTACT	1 cut(s)	2537

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.