Rw6G013100

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0008889
Physical Location & Seq
Forward (+)
24115930 .. 24134373
18444 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G013100.1

Sequence Viewer

Length: 3219 bp
ATGAAACCCAATTCAATGGTCACTCAATTTGGAACCCCTTCCTCTTCTTCTTTCACCCATAAACATTCTTGGACATATGATGTCTTTCTAAGTTTCAGAGGTCAGGATACACGCGGAAATTTTACAGACCATTTGTATAGCGCTTTGCGTCGACAGGGGATTAAGACCTTCGATAACGTTGAGGTTCCAAAAGGAGAAGTGATTGGCCCGGCGATTTTCAGAGCAATCGAAGAGTCAAGAATTTCTGTCATCATATTATCTCACAATTATGCAACTTCAAGGTGGTGTTTGGAGGAATTGGTGAAGATCCTTGGATGTAGAAACTCAAAGCAACAACAGGTTCTTCCTGTGTTCTACAAGGTTAGCCCCTCCGATGTAAGGAACCTGACAGGTCGTTTTGGTGAGGCATTAGCTGCGCATGAAATTGAGTTCAAGGATAAGATATATGAATGGAGAGCAGCTCTTACGGAAGTAGCAAATCTCTCTGGGTGGCATTTTCCGGAGGACGGGTTTGAGTCGAAGTTTATTCATGGGGTTGTTGAAGAGATTTCGAAACGAGTACATGTTACCTATTTGCATGTGGCAGATTATCCGGTAGGAATAGAGTCTCGTATACGTGATATGGATAGGCTTATAGGTGTTGGAGGGGATGATGATATTCGTATGGTCGGGATATGGGGGCCTGGTGGAATAGGTAAAACAACAATTGCCAAAGCTGTTTATAATTCAATTATTCACAAGTTCGAATGTAGCTGCTTCCTGGAAAATATTAGAGAAAGATCAGGGCAATATGGAGGCCTCGTGAAACTACAAAGATTTTTTCTTTCCCACATCCTAATGGGAAAAGAAATAAAATTAGCCACTTGCAATGAAGGAATGACAGTTATAAAGGAAATATTAAGCCACAAAAGGGTTCTGTTAGTTCTTGATGATGTGAATGACTTGGAACAGTTAAGAATATTAGCTGGAGGATCTCAGTGGTTTGGTTTGGGAAGCAGGGTTATTATTACAACAAGAGATAAGCATTTATTAATTGCTCATCACGTCAATCTAATATACAAGGTCAAAAAATTGGATCATCATGAAGCTTTTGAGCTCTTCAGTTACAATTGCTTCCCAAAGAAGGAACTTTCAAATGAGTCTGCGAAACTGGCAATTGATGCAGTACAGTACGCCCAGGGCCTTCCATTAGCTCTTGTAGCTTTAGGCTCAGTTCTTTGTGGTAGATCTATAGAGGAGTGGAAAGAAGCGAGAGATGGTAATTGGCAATTTGGTATCACAGGTCTGTATGACACTCTTAAAATAAGTATTGATGCATTGGAACATCAGGTTAGAGAAGTTTTCCTTGACATCGCATGTTTCTTTAGAGGTGAAAATAACAATCATGTGATACAGTTACTTAGAGGGGGTGGCGTCAATCCTAACATCAGTCTTAGTATCCTCCAAGAAAAGGCGGTGATAAGTATTAATGAAGAAAGCCATATCTGGATGCACAACTTGGTGGAGGTAATGGGAAAAAGAATAGTTCACATTGAGTCCCCAAATGAACCCTGCAAACGCAGTAGATTGTGGTCTTCTGATGATGTTTACGATGTATTAACTAAAGGCAAAGGAACAAACAAAGTCAAAGGTATCATGGTAAAGTTGCCTGAACCAGATGAGATAGTATTGAGTGCTGCAAGCTTTTCACGGATGAAAAATCTTAAATACTTCATAAACTGTAATGCATCTTTTTCGGGAAACATTGATTATCTCCCTGATGAGTTGAGGCTAATAGATTGGCCTGCATTTCCTTTCGAATCTTTACCAATCAATTTTATTCCGAGAAAGCTTGTTAAGCTCAGTATGCCTCGCAGCTGCATGTCACAACTGGGGGAGAGATTCAAGCACTCCAAAACTCTGAAATATATAAATCTGGAAAGCTGCCCATTCTTGACAGAAATCCCGAACTTGTCTGGATTTCCAAATTTAAGGCACTTGAATTTGAGCAACTGTACTGCTTTAGTTGAGCTTGATGATTCTATTGGATTCCTTAATAAGCTTGTGTTTTTAAGTCTATCAGGATGCCGTAATATTACTAAGTTTCCAAGAAGAATCGCCTTGAAATCTGCAAAACATATTACTCTTAGAGGTTGCAGAATGCTTGAGTCTTTCCCCGCAATTGTAAAAAGGATGGAGTGCTTGACAAGCTTAGATCTATCCGGCACTGCCATCAAAGAATTGCCTTCACAAATTGCATCTCTGATTAGCCTTGAAGAGTTAACTATAAGAGAATGTGACAACCTTACTACTCTTCCATGCAGCATATATGATTTGCAGAATTTGGGGTCTCTTGATCTCCAAGGCTGCTCAAAACTTGTTACGTTTCCAAAATGGTGTGCAAAATCAGTCCCAACATACTTGAACATTTCACATGACTTGACTTATCTAAATCTACGTGGCTGCAGGATGCTTGAGGAAATTCCTGAACTTCCACCAAAAGTGAAATGGGTAAATGCGGCTGATTGCTTATTGTTGGAACGATTTACGAAATTATCAAACATCTTGGAACAAAGAGAGTCGCAAATGATCAAATGTGTTTCACTGTTGAATTGTCAGAGACTGTGTGGAAGTCTGGCCAACAAAATGGAAGAGATTGAAGATACTTTACTGAATGAGGTCACTCTCTGCTCTCTGTTTCTCTCATGCAAGCAATCTGAATTCAACGTTGTTTTTCCAGGAGCTGAAGTTCCAAAGTGGTTCAACCATTGGAAGTATTTGAGTAATCCAGTTGATACATCTGAATTTTCTTTTGAAATCCCTCTCAATTTCAAATTGGGGGACAAAGGATTGGCTATATGTGCAGTGGCTACTTCTGTAGATGATCTTGGTCATTGCGCTTTCACAGCCAGAATTAGCATTCAGGAAGAAAGTAGTATTACACGTTCGTTTAGTTTTGAAGCAAAAGAAATGGTCTCAGCTCATGTGTGGCTGCTCTATATTCCATTGGTTAACTTTGCTCATCGTCAATCCTTGCCTTTTACCTGTCGAGTTGCTTTGGAACATACTAGCCAAGGTTCAGCGCGCTGTAAAAGCTATGGGGTGCACCTAGTAATGCCCCAAGGTGAATATGATGACTACATGGAAGATGACTACTCCGTCTGCTCATCTAGTGACGATGATAAGAGTAATATTTCAGACCGGAAAAAGAAAAAAGAGAAGATGAAGAAATGGAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1072

Amino Acids

121.41

Weight (kDa)

7.67

Isoelectric Point (pI)

49.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 26 - 189 5.8e-51 TIR domain
TIR_2 PF13676 28 - 123 1.7e-14 TIR domain
NB-ARC PF00931 217 - 375 6.4e-19 NB-ARC domain
WHD_ROQ1 PF23282 444 - 510 1.5e-14 Disease resistance protein Roq1-like, winged-helix domain
LRR_13 PF23286 703 - 787 1.2e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 728 - 786 3.6e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 723, 887
AasI GACNNNNNNGTC 1 cut(s) 3137
Acc16I TGCGCA 1 cut(s) 417
AccI GTMKAC 2 cut(s) 151, 613
AccII CGCG 2 cut(s) 114, 3064
AccIII TCCGGA 1 cut(s) 499
AciI CCGC 4 cut(s) 114, 1454, 2157, 2498
AclI AACGTT 2 cut(s) 177, 2706
AclWI GGATC 3 cut(s) 301, 979, 1083
AcoI YGGCCR 1 cut(s) 2616
AcsI RAATTY 8 cut(s) 118, 240, 1966, 1981, 2320, 2460, 2699, 2783
AcuI CTGAAG 2 cut(s) 1084, 2745
AcyI GRCGYC 1 cut(s) 1413
AfaI GTAC 4 cut(s) 561, 1167, 1172, 1996
AfeI AGCGCT 1 cut(s) 142
AfiI CCNNNNNNNGG 5 cut(s) 378, 506, 910, 1123, 1450
AflIII ACRYGT 2 cut(s) 562, 2921
AjiI CACGTC 1 cut(s) 1045
AjnI CCWGG 4 cut(s) 682, 759, 1176, 2716
AloI GAACNNNNNNTCC 2 cut(s) 2712, 2744
Alw21I GWGCWC 2 cut(s) 1098, 3087
Alw26I GTCTC 4 cut(s) 612, 2334, 2593, 2959
Alw44I GTGCAC 1 cut(s) 3083
AlwI GGATC 3 cut(s) 301, 979, 1083
AlwNI CAGNNNCTG 2 cut(s) 2602, 2723
Aor13HI TCCGGA 1 cut(s) 499
Aor51HI AGCGCT 1 cut(s) 142
AoxI GGCC 6 cut(s) 205, 680, 796, 1180, 1781, 2616
ApaLI GTGCAC 1 cut(s) 3083
ApoI RAATTY 8 cut(s) 118, 240, 1966, 1981, 2320, 2460, 2699, 2783
AseI ATTAAT 2 cut(s) 1031, 1467
Asp700I GAANNNNTTC 1 cut(s) 37
AspLEI GCGC 5 cut(s) 143, 418, 2879, 3064, 3066
AspS9I GGNCC 3 cut(s) 206, 680, 1180
AsuC2I CCSGG 1 cut(s) 209
AsuHPI GGTGA 6 cut(s) 46, 313, 413, 1382, 1468, 3116
AsuII TTCGAA 3 cut(s) 551, 744, 1797
BaeGI GKGCMC 1 cut(s) 3087
BalI TGGCCA 1 cut(s) 2618
BanII GRGCYC 1 cut(s) 1098
BarI GAAGNNNNNNTAC 2 cut(s) 2631, 2663
BauI CACGAG 1 cut(s) 800
BbsI GAAGAC 1 cut(s) 1566
Bbv12I GWGCWC 2 cut(s) 1098, 3087
BccI CCATC 3 cut(s) 1250, 2167, 2219
BceAI ACGGC 1 cut(s) 2052
BcgI CGANNNNNNTGC 2 cut(s) 1716, 1750
BciT130I CCWGG 4 cut(s) 684, 761, 1178, 2718
BciVI GTATCC 2 cut(s) 100, 1448
BclI TGATCA 1 cut(s) 2568
BcnI CCSGG 1 cut(s) 209
BcoDI GTCTC 4 cut(s) 612, 2334, 2593, 2959
BfaI CTAG 3 cut(s) 3048, 3089, 3150
BfmI CTRYAG 3 cut(s) 1230, 2443, 2856
BfoI RGCGCY 1 cut(s) 144
BfuI GTATCC 2 cut(s) 100, 1448
BglII AGATCT 2 cut(s) 1226, 2194
Bme1390I CCNGG 5 cut(s) 209, 684, 761, 1178, 2718
BmgBI CACGTC 1 cut(s) 1045
BmgT120I GGNCC 3 cut(s) 206, 680, 1180
BmiI GGNNCC 4 cut(s) 34, 186, 383, 681
BmrFI CCNGG 5 cut(s) 209, 684, 761, 1178, 2718
BmrI ACTGGG 1 cut(s) 1880
BmsI GCATC 7 cut(s) 1150, 1303, 1479, 1736, 2054, 2246, 2439
BmuI ACTGGG 1 cut(s) 1880
BpiI GAAGAC 1 cut(s) 1566
BplI GAGNNNNNCTC 2 cut(s) 445, 477
BpmI CTGGAG 1 cut(s) 987
Bpu14I TTCGAA 3 cut(s) 551, 744, 1797
BpuEI CTTGAG 2 cut(s) 2165, 2474
BpuMI CCSGG 1 cut(s) 209
BsaAI YACGTR 2 cut(s) 617, 2438
BsaHI GRCGYC 1 cut(s) 1413
BsaI GGTCTC 2 cut(s) 2334, 2959
BsaJI CCNNGG 6 cut(s) 310, 1176, 1177, 2341, 3052, 3100
BsaWI WCCGGW 3 cut(s) 499, 592, 3180
BsaXI ACNNNNNCTCC 6 cut(s) 494, 524, 786, 816, 2712, 2742
Bsc4I CCNNNNNNNGG 5 cut(s) 378, 506, 910, 1123, 1450
Bse1I ACTGG 3 cut(s) 1155, 1875, 2768
Bse3DI GCAATG 2 cut(s) 874, 2872
BseAI TCCGGA 1 cut(s) 499
BseBI CCWGG 4 cut(s) 684, 761, 1178, 2718
BseDI CCNNGG 6 cut(s) 310, 1176, 1177, 2341, 3052, 3100
BseGI GGATG 8 cut(s) 320, 655, 831, 1494, 1698, 2069, 2178, 2454
BseLI CCNNNNNNNGG 5 cut(s) 378, 506, 910, 1123, 1450
BseMI GCAATG 2 cut(s) 874, 2872
BseMII CTCAG 4 cut(s) 989, 1224, 1855, 2970
BseNI ACTGG 3 cut(s) 1155, 1875, 2768
BsePI GCGCGC 1 cut(s) 3062
BseRI GAGGAG 1 cut(s) 1250
BseSI GKGCMC 1 cut(s) 3087
BsgI GTGCAG 1 cut(s) 2862
Bsh1236I CGCG 2 cut(s) 114, 3064
BshFI GGCC 6 cut(s) 207, 682, 798, 1182, 1783, 2618
BsiHKAI GWGCWC 2 cut(s) 1098, 3087
BsiSI CCGG 5 cut(s) 209, 500, 593, 2202, 3181
BslFI GGGAC 3 cut(s) 1522, 2375, 2834
BslI CCNNNNNNNGG 5 cut(s) 378, 506, 910, 1123, 1450
BsmAI GTCTC 4 cut(s) 612, 2334, 2593, 2959
BsmFI GGGAC 3 cut(s) 1522, 2375, 2834
BsmI GAATGC 2 cut(s) 2145, 2898
BsnI GGCC 6 cut(s) 207, 682, 798, 1182, 1783, 2618
Bso31I GGTCTC 2 cut(s) 2334, 2959
Bsp119I TTCGAA 3 cut(s) 551, 744, 1797
Bsp1286I GDGCHC 2 cut(s) 1098, 3087
Bsp13I TCCGGA 1 cut(s) 499
Bsp143I GATC 9 cut(s) 306, 779, 971, 1075, 1226, 2194, 2335, 2568, 2863
BspACI CCGC 4 cut(s) 114, 1454, 2157, 2498
BspANI GGCC 6 cut(s) 207, 682, 798, 1182, 1783, 2618
BspCNI CTCAG 4 cut(s) 988, 1223, 1854, 2969
BspEI TCCGGA 1 cut(s) 499
BspFNI CGCG 2 cut(s) 114, 3064
BspHI TCATGA 1 cut(s) 1081
BspLI GGNNCC 4 cut(s) 34, 186, 383, 681
BspMAI CTGCAG 1 cut(s) 2447
BspPI GGATC 3 cut(s) 301, 979, 1083
BspQI GCTCTTC 1 cut(s) 1103
BspT104I TTCGAA 3 cut(s) 551, 744, 1797
BspTNI GGTCTC 2 cut(s) 2334, 2959
BsrDI GCAATG 2 cut(s) 874, 2872
BsrI ACTGG 3 cut(s) 1155, 1875, 2768
BssECI CCNNGG 6 cut(s) 310, 1176, 1177, 2341, 3052, 3100
BssHII GCGCGC 1 cut(s) 3062
BssMI GATC 9 cut(s) 306, 779, 971, 1075, 1226, 2194, 2335, 2568, 2863
BssNAI GTATAC 1 cut(s) 614
BssNI GRCGYC 1 cut(s) 1413
BssSI CACGAG 1 cut(s) 800
BssT1I CCWWGG 4 cut(s) 310, 2341, 3052, 3100
Bst1107I GTATAC 1 cut(s) 614
Bst2BI CACGAG 1 cut(s) 800
Bst2UI CCWGG 4 cut(s) 684, 761, 1178, 2718
Bst4CI ACNGT 8 cut(s) 883, 951, 1170, 1395, 1721, 1994, 2586, 2604
Bst6I CTCTTC 7 cut(s) 49, 225, 537, 1103, 2250, 2298, 2625
BstACI GRCGYC 1 cut(s) 1413
BstAPI GCANNNNNTGC 2 cut(s) 413, 1160
BstBAI YACGTR 2 cut(s) 617, 2438
BstBI TTCGAA 3 cut(s) 551, 744, 1797
BstC8I GCNNGC 4 cut(s) 1681, 1785, 2690, 3064
BstF5I GGATG 8 cut(s) 320, 655, 831, 1494, 1698, 2069, 2178, 2454
BstFNI CGCG 2 cut(s) 114, 3064
BstH2I RGCGCY 1 cut(s) 144
BstHHI GCGC 5 cut(s) 143, 418, 2879, 3064, 3066
BstKTI GATC 9 cut(s) 309, 782, 974, 1078, 1229, 2197, 2338, 2571, 2866
BstMAI GTCTC 4 cut(s) 612, 2334, 2593, 2959
BstMBI GATC 9 cut(s) 306, 779, 971, 1075, 1226, 2194, 2335, 2568, 2863
BstNI CCWGG 4 cut(s) 684, 761, 1178, 2718
BstNSI RCATGY 4 cut(s) 566, 581, 1359, 1864
BstSCI CCNGG 5 cut(s) 207, 682, 759, 1176, 2716
BstSFI CTRYAG 3 cut(s) 1230, 2443, 2856
BstSLI GKGCMC 1 cut(s) 3087
BstUI CGCG 2 cut(s) 114, 3064
BstV2I GAAGAC 1 cut(s) 1566
BstX2I RGATCY 4 cut(s) 306, 971, 1226, 2194
BstXI CCANNNNNNTGG 2 cut(s) 16, 2626
BstYI RGATCY 4 cut(s) 306, 971, 1226, 2194
BstZ17I GTATAC 1 cut(s) 614
BsuI GTATCC 2 cut(s) 100, 1448
BsuRI GGCC 6 cut(s) 207, 682, 798, 1182, 1783, 2618
BtgZI GCGATG 1 cut(s) 1336
BtrI CACGTC 1 cut(s) 1045
BtsCI GGATG 8 cut(s) 320, 655, 831, 1494, 1698, 2069, 2178, 2454
BtsI GCAGTG 2 cut(s) 2205, 2850
BtsIMutI CAGTG 4 cut(s) 983, 2205, 2582, 2850
Cac8I GCNNGC 4 cut(s) 1681, 1785, 2690, 3064
CaiI CAGNNNCTG 2 cut(s) 2602, 2723
CciI TCATGA 1 cut(s) 1081
CfoI GCGC 5 cut(s) 143, 418, 2879, 3064, 3066
Cfr13I GGNCC 3 cut(s) 206, 680, 1180
CseI GACGC 2 cut(s) 137, 1402
Csp6I GTAC 4 cut(s) 560, 1166, 1171, 1995
CviQI GTAC 4 cut(s) 560, 1166, 1171, 1995
DpnI GATC 9 cut(s) 308, 781, 973, 1077, 1228, 2196, 2337, 2570, 2865
DpnII GATC 9 cut(s) 306, 779, 971, 1075, 1226, 2194, 2335, 2568, 2863
DrdI GACNNNNNNGTC 1 cut(s) 3137
DseDI GACNNNNNNGTC 1 cut(s) 3137
EaeI YGGCCR 1 cut(s) 2616
Eam1104I CTCTTC 7 cut(s) 49, 225, 537, 1103, 2250, 2298, 2625
EarI CTCTTC 7 cut(s) 49, 225, 537, 1103, 2250, 2298, 2625
Ecl136II GAGCTC 1 cut(s) 1096
Eco130I CCWWGG 4 cut(s) 310, 2341, 3052, 3100
Eco147I AGGCCT 1 cut(s) 798
Eco24I GRGCYC 1 cut(s) 1098
Eco31I GGTCTC 2 cut(s) 2334, 2959
Eco47III AGCGCT 1 cut(s) 142
Eco53kI GAGCTC 1 cut(s) 1096
Eco57I CTGAAG 2 cut(s) 1084, 2745
EcoICRI GAGCTC 1 cut(s) 1096
EcoO109I RGGNCCY 2 cut(s) 680, 1180
EcoRI GAATTC 1 cut(s) 2699
EcoRII CCWGG 4 cut(s) 682, 759, 1176, 2716
EcoT14I CCWWGG 4 cut(s) 310, 2341, 3052, 3100
EcoT22I ATGCAT 2 cut(s) 1318, 1729
EcoT38I GRGCYC 1 cut(s) 1098
ErhI CCWWGG 4 cut(s) 310, 2341, 3052, 3100
FalI AAGNNNNNCTT 2 cut(s) 1329, 1361
FaqI GGGAC 3 cut(s) 1522, 2375, 2834
FauI CCCGC 1 cut(s) 2164
FauNDI CATATG 1 cut(s) 76
FbaI TGATCA 1 cut(s) 2568
FblI GTMKAC 2 cut(s) 151, 613
FokI GGATG 8 cut(s) 327, 662, 818, 1501, 1705, 2076, 2185, 2461
FriOI GRGCYC 1 cut(s) 1098
FspBI CTAG 3 cut(s) 3048, 3089, 3150
FspI TGCGCA 1 cut(s) 417
GlaI GCGC 5 cut(s) 142, 417, 2878, 3063, 3065
GsuI CTGGAG 1 cut(s) 987
HaeII RGCGCY 1 cut(s) 144
HaeIII GGCC 6 cut(s) 207, 682, 798, 1182, 1783, 2618
HapII CCGG 5 cut(s) 209, 500, 593, 2202, 3181
HgaI GACGC 2 cut(s) 137, 1402
HhaI GCGC 5 cut(s) 143, 418, 2879, 3064, 3066
Hin1I GRCGYC 1 cut(s) 1413
Hin6I GCGC 5 cut(s) 141, 416, 2877, 3062, 3064
HinP1I GCGC 5 cut(s) 141, 416, 2877, 3062, 3064
HincII GTYRAC 3 cut(s) 152, 2262, 2992
HindII GTYRAC 3 cut(s) 152, 2262, 2992
HindIII AAGCTT 5 cut(s) 1086, 1681, 1829, 2039, 2188
HpaI GTTAAC 2 cut(s) 2262, 2992
HpaII CCGG 5 cut(s) 209, 500, 593, 2202, 3181
HphI GGTGA 6 cut(s) 46, 313, 413, 1382, 1468, 3116
Hpy166II GTNNAC 7 cut(s) 152, 614, 1528, 1588, 2262, 2992, 3085
Hpy8I GTNNAC 7 cut(s) 152, 614, 1528, 1588, 2262, 2992, 3085
Hpy99I CGWCG 1 cut(s) 153
HpyAV CCTTC 6 cut(s) 48, 178, 866, 1117, 1193, 2235
HpyCH4III ACNGT 8 cut(s) 883, 951, 1170, 1395, 1721, 1994, 2586, 2604
HpyCH4IV ACGT 7 cut(s) 177, 616, 1044, 2363, 2437, 2706, 2923
HpySE526I ACGT 7 cut(s) 177, 616, 1044, 2363, 2437, 2706, 2923
Hsp92I GRCGYC 1 cut(s) 1413
HspAI GCGC 5 cut(s) 141, 416, 2877, 3062, 3064
Kpn2I TCCGGA 1 cut(s) 499
Ksp22I TGATCA 1 cut(s) 2568
KspAI GTTAAC 2 cut(s) 2262, 2992
Kzo9I GATC 9 cut(s) 306, 779, 971, 1075, 1226, 2194, 2335, 2568, 2863
LguI GCTCTTC 1 cut(s) 1103
LmnI GCTCC 1 cut(s) 2720
LweI GCATC 7 cut(s) 1150, 1303, 1479, 1736, 2054, 2246, 2439
MaeI CTAG 3 cut(s) 3048, 3089, 3150
MaeII ACGT 7 cut(s) 177, 616, 1044, 2363, 2437, 2706, 2923
MaeIII GTNAC 9 cut(s) 19, 565, 1103, 1395, 1863, 2276, 2359, 2659, 3152
MalI GATC 9 cut(s) 308, 781, 973, 1077, 1228, 2196, 2337, 2570, 2865
MboI GATC 9 cut(s) 306, 779, 971, 1075, 1226, 2194, 2335, 2568, 2863
MfeI CAATTG 4 cut(s) 705, 1108, 1155, 2160
MflI RGATCY 4 cut(s) 306, 971, 1226, 2194
MhlI GDGCHC 2 cut(s) 1098, 3087
MlsI TGGCCA 1 cut(s) 2618
MluNI TGGCCA 1 cut(s) 2618
MlyI GAGTC 7 cut(s) 242, 524, 614, 1148, 1544, 2156, 2567
MmeI TCCRAC 2 cut(s) 622, 2496
Mox20I TGGCCA 1 cut(s) 2618
Mph1103I ATGCAT 2 cut(s) 1318, 1729
MroI TCCGGA 1 cut(s) 499
MroXI GAANNNNTTC 1 cut(s) 37
MscI TGGCCA 1 cut(s) 2618
MslI CAYNNNNRTG 2 cut(s) 267, 836
Msp20I TGGCCA 1 cut(s) 2618
MspA1I CMGCKG 1 cut(s) 1857
MspI CCGG 5 cut(s) 209, 500, 593, 2202, 3181
MspR9I CCNGG 5 cut(s) 209, 684, 761, 1178, 2718
MunI CAATTG 4 cut(s) 705, 1108, 1155, 2160
Mva1269I GAATGC 2 cut(s) 2145, 2898
MvaI CCWGG 4 cut(s) 684, 761, 1178, 2718
MvnI CGCG 2 cut(s) 114, 3064
NciI CCSGG 1 cut(s) 209
NdeI CATATG 1 cut(s) 76
NdeII GATC 9 cut(s) 306, 779, 971, 1075, 1226, 2194, 2335, 2568, 2863
NlaIV GGNNCC 4 cut(s) 34, 186, 383, 681
NmuCI GTSAC 5 cut(s) 19, 1863, 2276, 2659, 3152
NsbI TGCGCA 1 cut(s) 417
NsiI ATGCAT 2 cut(s) 1318, 1729
NspI RCATGY 4 cut(s) 566, 581, 1359, 1864
NspV TTCGAA 3 cut(s) 551, 744, 1797
PagI TCATGA 1 cut(s) 1081
PasI CCCWGGG 1 cut(s) 1177
PauI GCGCGC 1 cut(s) 3062
PceI AGGCCT 1 cut(s) 798
PciI ACATGT 1 cut(s) 562
PciSI GCTCTTC 1 cut(s) 1103
PctI GAATGC 2 cut(s) 2145, 2898
PdmI GAANNNNTTC 1 cut(s) 37
PfeI GAWTC 5 cut(s) 1799, 1881, 2018, 2028, 2094
PfoI TCCNGGA 2 cut(s) 759, 2716
PleI GAGTC 7 cut(s) 241, 523, 613, 1147, 1543, 2155, 2566
PpsI GAGTC 7 cut(s) 241, 523, 613, 1147, 1543, 2155, 2566
Ppu21I YACGTR 2 cut(s) 617, 2438
PscI ACATGT 1 cut(s) 562
PshBI ATTAAT 2 cut(s) 1031, 1467
PsiI TTATAA 2 cut(s) 723, 887
Psp124BI GAGCTC 1 cut(s) 1098
Psp1406I AACGTT 2 cut(s) 177, 2706
Psp6I CCWGG 4 cut(s) 682, 759, 1176, 2716
PspGI CCWGG 4 cut(s) 682, 759, 1176, 2716
PspN4I GGNNCC 4 cut(s) 34, 186, 383, 681
PspPI GGNCC 3 cut(s) 206, 680, 1180
PsrI GAACNNNNNNTAC 2 cut(s) 2908, 2940
PstI CTGCAG 1 cut(s) 2447
PstNI CAGNNNCTG 2 cut(s) 2602, 2723
PsuI RGATCY 4 cut(s) 306, 971, 1226, 2194
PteI GCGCGC 1 cut(s) 3062
PvuII CAGCTG 1 cut(s) 1857
RsaI GTAC 4 cut(s) 561, 1167, 1172, 1996
RsaNI GTAC 4 cut(s) 560, 1166, 1171, 1995
RseI CAYNNNNRTG 2 cut(s) 267, 836
SacI GAGCTC 1 cut(s) 1098
SalI GTCGAC 1 cut(s) 150
SapI GCTCTTC 1 cut(s) 1103
Sau3AI GATC 9 cut(s) 306, 779, 971, 1075, 1226, 2194, 2335, 2568, 2863
Sau96I GGNCC 3 cut(s) 206, 680, 1180
SchI GAGTC 7 cut(s) 242, 524, 614, 1148, 1544, 2156, 2567
ScrFI CCNGG 5 cut(s) 209, 684, 761, 1178, 2718
SduI GDGCHC 2 cut(s) 1098, 3087
SfaNI GCATC 7 cut(s) 1150, 1303, 1479, 1736, 2054, 2246, 2439
SfcI CTRYAG 3 cut(s) 1230, 2443, 2856
SfuI TTCGAA 3 cut(s) 551, 744, 1797
SmiMI CAYNNNNRTG 2 cut(s) 267, 836
SmlI CTYRAG 2 cut(s) 2144, 2453
SmoI CTYRAG 2 cut(s) 2144, 2453
SseBI AGGCCT 1 cut(s) 798
SsiI CCGC 4 cut(s) 114, 1454, 2157, 2498
SspI AATATT 5 cut(s) 769, 897, 960, 2074, 3172
SspMI CTAG 3 cut(s) 3048, 3089, 3150
SstI GAGCTC 1 cut(s) 1098
StuI AGGCCT 1 cut(s) 798
StyD4I CCNGG 5 cut(s) 207, 682, 759, 1176, 2716
StyI CCWWGG 4 cut(s) 310, 2341, 3052, 3100
TaaI ACNGT 8 cut(s) 883, 951, 1170, 1395, 1721, 1994, 2586, 2604
TaiI ACGT 7 cut(s) 180, 619, 1047, 2366, 2440, 2709, 2926
TaqI TCGA 8 cut(s) 151, 171, 228, 518, 551, 744, 1797, 3028
TatI WGTACW 3 cut(s) 559, 1165, 1994
TauI GCSGC 1 cut(s) 2501
TfiI GAWTC 5 cut(s) 1799, 1881, 2018, 2028, 2094
TscAI CASTG 4 cut(s) 983, 2212, 2589, 2850
TseFI GTSAC 5 cut(s) 19, 1863, 2276, 2659, 3152
Tsp45I GTSAC 5 cut(s) 19, 1863, 2276, 2659, 3152
TspGWI ACGGA 3 cut(s) 482, 1705, 3127
TspRI CASTG 4 cut(s) 983, 2212, 2589, 2850
VneI GTGCAC 1 cut(s) 3083
VspI ATTAAT 2 cut(s) 1031, 1467
XapI RAATTY 8 cut(s) 118, 240, 1966, 1981, 2320, 2460, 2699, 2783
XceI RCATGY 4 cut(s) 566, 581, 1359, 1864
XcmI CCANNNNNNNNNTGG 1 cut(s) 2484
XmiI GTMKAC 2 cut(s) 151, 613
XmnI GAANNNNTTC 1 cut(s) 37
XspI CTAG 3 cut(s) 3048, 3089, 3150
Zsp2I ATGCAT 2 cut(s) 1318, 1729
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.