RchiOBHm_Chr6g0300741

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0012585
Physical Location & Seq
Forward (+)
61492292 .. 61492948
657 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27009

Sequence Viewer

Length: 657 bp
ATGTCGTTTGCTCAGAAGGTGATGGTTTTGACGGTTGGCTCCGGGAGTTGGAGAAGCATTTGGTACCCCGGCTACCATTCTGAGCAAGAAAATGGGATTTATGTAAATGGATATCTCCATTGGATAGGCCAGTGTGGTAAGGGTTCTTTGGTGATACATTCCTTTAACCTTGAAAGTGAGAGTTATGAGCAGTTACCAATGCCACCTTGTTGTTTTGATCCCCATAAAGCTCAGTTGAACCTTGGAGTCTTTAACGGCTGCCTTTCTATAACTGTTCGCTCGAGCTATTATATCAGGATTTGGGTGATGAAGAATTATGGTGTTAAGGAGTACTCTTGGACCAGAGAGGTTGACATTAGAGAAATACTGGGTGGTCTTGTAAAACGTAGTTGTGATTATCCTACTCGTGTTCTAGAATACACAAAGGAGAGGCAAGTCTTGGTGTTAGATAATAGGTTGCAGAGTTATAGTACTCCAAACGAGGGCTTTGTGAAGAATGAGGTTGATGGGATAGAAAATATTCATGATGCTGCATATGTCCATATTCAAAGCTTTGTTCTGCTTAAAGATCTCATCAGGGGTTCGAGCATATATGTTCAAAGATTTAGGCATGATAAGAAGTCTGGCAAGTTGGTTGCTCATAATCCTAGGGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 RVT_3 zf-RVT

Protein Analysis

218

Amino Acids

25.09

Weight (kDa)

8.72

Isoelectric Point (pI)

42.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 4 - 112 8.2e-12 F-box associated beta propeller domain
FBA_1 PF07734 8 - 150 1.7e-10 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 63
AccB1I GGYRCC 1 cut(s) 63
AclWI GGATC 1 cut(s) 212
AfaI GTAC 3 cut(s) 65, 332, 472
AfiI CCNNNNNNNGG 2 cut(s) 48, 482
AgsI TTSAA 4 cut(s) 173, 238, 548, 599
AluBI AGCT 3 cut(s) 230, 285, 552
AluI AGCT 3 cut(s) 230, 285, 552
AlwI GGATC 1 cut(s) 212
Ama87I CYCGRG 1 cut(s) 280
AoxI GGCC 1 cut(s) 127
ApeKI GCWGC 2 cut(s) 258, 530
Asp700I GAANNNNTTC 1 cut(s) 519
Asp718I GGTACC 1 cut(s) 63
AspA2I CCTAGG 1 cut(s) 647
AspS9I GGNCC 1 cut(s) 339
AsuC2I CCSGG 2 cut(s) 43, 69
AsuHPI GGTGA 3 cut(s) 31, 163, 316
AvaI CYCGRG 1 cut(s) 280
AvaII GGWCC 1 cut(s) 339
AvrII CCTAGG 1 cut(s) 647
BanI GGYRCC 1 cut(s) 63
BauI CACGAG 1 cut(s) 405
BbvI GCAGC 2 cut(s) 245, 517
BccI CCATC 2 cut(s) 16, 500
BceAI ACGGC 1 cut(s) 271
BcnI CCSGG 2 cut(s) 43, 69
BfaI CTAG 2 cut(s) 413, 648
BglII AGATCT 1 cut(s) 568
BisI GCNGC 2 cut(s) 259, 531
BlnI CCTAGG 1 cut(s) 647
BlsI GCNGC 2 cut(s) 260, 532
BmcAI AGTACT 2 cut(s) 332, 472
Bme1390I CCNGG 2 cut(s) 43, 69
Bme18I GGWCC 1 cut(s) 339
BmeT110I CYCGRG 1 cut(s) 280
BmgT120I GGNCC 1 cut(s) 339
BmiI GGNNCC 2 cut(s) 40, 65
BmrFI CCNGG 2 cut(s) 43, 69
BmrI ACTGGG 1 cut(s) 377
BmsI GCATC 1 cut(s) 517
BmuI ACTGGG 1 cut(s) 377
BpuMI CCSGG 2 cut(s) 43, 69
BsaJI CCNNGG 3 cut(s) 67, 241, 647
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 482
Bse1I ACTGG 2 cut(s) 130, 372
BseDI CCNNGG 3 cut(s) 67, 241, 647
BseLI CCNNNNNNNGG 2 cut(s) 48, 482
BseMII CTCAG 3 cut(s) 26, 72, 245
BseNI ACTGG 2 cut(s) 130, 372
BseXI GCAGC 2 cut(s) 245, 517
BshFI GGCC 1 cut(s) 129
BshNI GGYRCC 1 cut(s) 63
BsiHKCI CYCGRG 1 cut(s) 280
BsiSI CCGG 2 cut(s) 42, 69
BslI CCNNNNNNNGG 2 cut(s) 48, 482
BsnI GGCC 1 cut(s) 129
BsoBI CYCGRG 1 cut(s) 280
Bsp143I GATC 2 cut(s) 217, 568
BspANI GGCC 1 cut(s) 129
BspCNI CTCAG 3 cut(s) 25, 73, 244
BspHI TCATGA 1 cut(s) 523
BspLI GGNNCC 2 cut(s) 40, 65
BspPI GGATC 1 cut(s) 212
BspT107I GGYRCC 1 cut(s) 63
BsrI ACTGG 2 cut(s) 130, 372
BssECI CCNNGG 3 cut(s) 67, 241, 647
BssMI GATC 2 cut(s) 217, 568
BssSI CACGAG 1 cut(s) 405
BssT1I CCWWGG 2 cut(s) 241, 647
Bst2BI CACGAG 1 cut(s) 405
Bst4CI ACNGT 2 cut(s) 34, 274
BstDEI CTNAG 3 cut(s) 12, 81, 231
BstKTI GATC 2 cut(s) 220, 571
BstMBI GATC 2 cut(s) 217, 568
BstSCI CCNGG 2 cut(s) 41, 67
BstV1I GCAGC 2 cut(s) 245, 517
BstX2I RGATCY 1 cut(s) 568
BstYI RGATCY 1 cut(s) 568
BsuRI GGCC 1 cut(s) 129
BtsIMutI CAGTG 1 cut(s) 137
CciI TCATGA 1 cut(s) 523
Cfr13I GGNCC 1 cut(s) 339
Csp6I GTAC 3 cut(s) 64, 331, 471
CviAII CATG 2 cut(s) 524, 611
CviJI RGCY 8 cut(s) 39, 72, 129, 230, 258, 285, 486, 552
CviKI_1 RGCY 8 cut(s) 39, 72, 129, 230, 258, 285, 486, 552
CviQI GTAC 3 cut(s) 64, 331, 471
DdeI CTNAG 3 cut(s) 12, 81, 231
DpnI GATC 2 cut(s) 219, 570
DpnII GATC 2 cut(s) 217, 568
Eco130I CCWWGG 2 cut(s) 241, 647
Eco32I GATATC 1 cut(s) 113
Eco47I GGWCC 1 cut(s) 339
Eco88I CYCGRG 1 cut(s) 280
EcoRV GATATC 1 cut(s) 113
EcoT14I CCWWGG 2 cut(s) 241, 647
ErhI CCWWGG 2 cut(s) 241, 647
FaeI CATG 2 cut(s) 527, 614
FatI CATG 2 cut(s) 523, 610
FauNDI CATATG 1 cut(s) 535
Fnu4HI GCNGC 2 cut(s) 259, 531
Fsp4HI GCNGC 2 cut(s) 259, 531
FspBI CTAG 2 cut(s) 413, 648
GluI GCNGC 2 cut(s) 259, 531
HaeIII GGCC 1 cut(s) 129
HapII CCGG 2 cut(s) 42, 69
Hin1II CATG 2 cut(s) 527, 614
HincII GTYRAC 1 cut(s) 352
HindII GTYRAC 1 cut(s) 352
HindIII AAGCTT 1 cut(s) 550
HinfI GANTC 1 cut(s) 246
HpaII CCGG 2 cut(s) 42, 69
HphI GGTGA 3 cut(s) 31, 163, 316
Hpy166II GTNNAC 1 cut(s) 352
Hpy188I TCNGA 2 cut(s) 15, 82
Hpy188III TCNNGA 3 cut(s) 295, 413, 524
Hpy8I GTNNAC 1 cut(s) 352
HpyAV CCTTC 1 cut(s) 10
HpyCH4III ACNGT 2 cut(s) 34, 274
HpyCH4IV ACGT 1 cut(s) 385
HpyCH4V TGCA 2 cut(s) 460, 533
HpyF3I CTNAG 3 cut(s) 12, 81, 231
HpySE526I ACGT 1 cut(s) 385
Hsp92II CATG 2 cut(s) 527, 614
KpnI GGTACC 1 cut(s) 67
Kzo9I GATC 2 cut(s) 217, 568
LmnI GCTCC 1 cut(s) 44
LpnPI CCDG 8 cut(s) 55, 82, 143, 280, 353, 355, 562, 609
Lsp1109I GCAGC 2 cut(s) 245, 517
LweI GCATC 1 cut(s) 517
MaeI CTAG 2 cut(s) 413, 648
MaeII ACGT 1 cut(s) 385
MaeIII GTNAC 1 cut(s) 192
MalI GATC 2 cut(s) 219, 570
MboI GATC 2 cut(s) 217, 568
MboII GAAGA 2 cut(s) 322, 505
MflI RGATCY 1 cut(s) 568
MluCI AATT 1 cut(s) 313
MlyI GAGTC 1 cut(s) 255
MmeI TCCRAC 1 cut(s) 29
MnlI CCTC 4 cut(s) 340, 423, 475, 493
MroXI GAANNNNTTC 1 cut(s) 519
MseI TTAA 4 cut(s) 165, 252, 324, 564
MspI CCGG 2 cut(s) 42, 69
MspR9I CCNGG 2 cut(s) 43, 69
NciI CCSGG 2 cut(s) 43, 69
NdeI CATATG 1 cut(s) 535
NdeII GATC 2 cut(s) 217, 568
NlaIII CATG 2 cut(s) 527, 614
NlaIV GGNNCC 2 cut(s) 40, 65
PaeR7I CTCGAG 1 cut(s) 280
PagI TCATGA 1 cut(s) 523
PdmI GAANNNNTTC 1 cut(s) 519
PfoI TCCNGGA 1 cut(s) 41
PkrI GCNGC 2 cut(s) 260, 532
PleI GAGTC 1 cut(s) 254
PpsI GAGTC 1 cut(s) 254
PspN4I GGNNCC 2 cut(s) 40, 65
PspPI GGNCC 1 cut(s) 339
PspXI VCTCGAGB 1 cut(s) 280
PsuI RGATCY 1 cut(s) 568
RsaI GTAC 3 cut(s) 65, 332, 472
RsaNI GTAC 3 cut(s) 64, 331, 471
SaqAI TTAA 4 cut(s) 165, 252, 324, 564
SatI GCNGC 2 cut(s) 259, 531
Sau3AI GATC 2 cut(s) 217, 568
Sau96I GGNCC 1 cut(s) 339
ScaI AGTACT 2 cut(s) 332, 472
SchI GAGTC 1 cut(s) 255
ScrFI CCNGG 2 cut(s) 43, 69
SfaNI GCATC 1 cut(s) 517
Sfr274I CTCGAG 1 cut(s) 280
SinI GGWCC 1 cut(s) 339
SlaI CTCGAG 1 cut(s) 280
SmlI CTYRAG 1 cut(s) 280
SmoI CTYRAG 1 cut(s) 280
Sse9I AATT 1 cut(s) 313
SspI AATATT 1 cut(s) 520
SspMI CTAG 2 cut(s) 413, 648
StyD4I CCNGG 2 cut(s) 41, 67
StyI CCWWGG 2 cut(s) 241, 647
TaaI ACNGT 2 cut(s) 34, 274
TaiI ACGT 1 cut(s) 388
TaqI TCGA 2 cut(s) 281, 584
TasI AATT 1 cut(s) 313
TatI WGTACW 2 cut(s) 330, 470
Tru1I TTAA 4 cut(s) 165, 252, 324, 564
Tru9I TTAA 4 cut(s) 165, 252, 324, 564
TscAI CASTG 1 cut(s) 137
TseI GCWGC 2 cut(s) 258, 530
TspDTI ATGAA 2 cut(s) 323, 512
TspRI CASTG 1 cut(s) 137
VpaK11BI GGWCC 1 cut(s) 339
XbaI TCTAGA 1 cut(s) 412
XhoI CTCGAG 1 cut(s) 280
XmaJI CCTAGG 1 cut(s) 647
XmnI GAANNNNTTC 1 cut(s) 519
XspI CTAG 2 cut(s) 413, 648
ZrmI AGTACT 2 cut(s) 332, 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.