Rmu_sc0006723.1_g000005

F-box protein At3g07870-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006723.1
OG0012585
Physical Location & Seq
Reverse (-)
34867 .. 36439
1573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006723.1_g000005.1.cds

Sequence Viewer

Length: 1362 bp
atgaagaataccgagctatgccatagccgccgacctcagaagcaacatgagaaggaagacgacgatgatcagaaaccctgccttgtacaattgccagaccatataattgccgatatctcctgcaaaatcccaatcaagaccctcatgaaatgcaggcatggctgcaaatctttgcgatctttgttcgcagacccgcaatttaccgaagaactattctctcgaacacgcccttgccatttggtctcgagcctcaattcggataggtacttcttggtcgacttcaacgagcggaggccaaacggcgtcgcagcactggatctttctatagactcagcgactcctagcagcgcaatcatggtgggatcttgcaagggcttggtctgcttatacaagatgtactgcccttacggtgtttacatatgcaattcaatgactggtgattccagtttcctttattggcctttatctctagaggaggagttaactcctgtagagtccactcctacaagacacagtgaggaaattttggagccatctttttgtggattcgggttgggatatagtaccataagtgatgtatacaaaattgttctaattgcttattcaagaaaaatgaggatggggattcggcctccaggtgagattgcgtatggacctcgggaggtgatggttttgactgtggactcagagggttggagacatgttggggaatttgatatgaacaattttgatcatgtacatggagtttatattcatgattctcttcattggactggcctgccggtaaagggtcctcgtttgatatatgcatttgacgttggaagggagtgtttccgagagctaacagtaccttgttggacttcttcggagagccaaatgactagattcaaccttggaatcttgaatgattgcctctctataactgtttgttggagagctagtatccatgtttgggtgatgaagcattacggtgtcgaggactcttggaccaaagagcttgacattagtgtggaatatggtccatcctcgtttggtggttacgcacaaattttgattttttcaccagaatggcaagtcctgttgtggcagggtaggttgcaggctcatactgctggaagtgagggctttgttgggcttcaggttgatgggatatcatcttcggttgacaaagcctatccccatgttccaagctttatttcacttaaagatgttatcaggtgtcagaaccctgaggggcaaggaaatgtgcaaaccactgcagtagatcatgggaatgctaaggaagataaacagaatttgcaatctaatgctcatggattaaatcaagggaatgtgcaatctgtaactataccagtcgattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

453

Amino Acids

50.93

Weight (kDa)

5.71

Isoelectric Point (pI)

45.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 289
AccI GTMKAC 2 cut(s) 276, 579
AciI CCGC 3 cut(s) 28, 194, 289
AclWI GGATC 2 cut(s) 324, 370
AcsI RAATTY 4 cut(s) 522, 710, 1047, 1294
AcuI CTGAAG 1 cut(s) 1121
AcyI GRCGYC 1 cut(s) 303
AfaI GTAC 6 cut(s) 87, 266, 398, 565, 738, 849
AfiI CCNNNNNNNGG 3 cut(s) 256, 788, 952
AflIII ACRYGT 1 cut(s) 700
AgsI TTSAA 5 cut(s) 283, 429, 606, 889, 904
AjnI CCWGG 1 cut(s) 634
AjuI GAANNNNNNNTTGG 2 cut(s) 1180, 1212
AluBI AGCT 5 cut(s) 16, 841, 938, 997, 1191
AluI AGCT 5 cut(s) 16, 841, 938, 997, 1191
Alw26I GTCTC 2 cut(s) 247, 691
AlwI GGATC 2 cut(s) 324, 370
Ama87I CYCGRG 2 cut(s) 244, 657
AoxI GGCC 4 cut(s) 293, 458, 629, 775
ApeKI GCWGC 3 cut(s) 162, 308, 345
ApoI RAATTY 4 cut(s) 522, 710, 1047, 1294
AspLEI GCGC 1 cut(s) 350
AspS9I GGNCC 4 cut(s) 653, 791, 987, 1019
AsuHPI GGTGA 5 cut(s) 449, 650, 676, 967, 1053
AvaI CYCGRG 2 cut(s) 244, 657
AvaII GGWCC 4 cut(s) 653, 791, 987, 1019
AxyI CCTNAGG 1 cut(s) 1230
BbsI GAAGAC 1 cut(s) 63
BbvI GCAGC 3 cut(s) 149, 320, 357
BccI CCATC 5 cut(s) 541, 613, 661, 1030, 1139
BceAI ACGGC 1 cut(s) 316
BciT130I CCWGG 1 cut(s) 636
BciVI GTATCC 1 cut(s) 953
BclI TGATCA 2 cut(s) 67, 730
BcoDI GTCTC 2 cut(s) 247, 691
BfaI CTAG 4 cut(s) 342, 470, 882, 939
BfmI CTRYAG 3 cut(s) 324, 489, 1257
BfuI GTATCC 1 cut(s) 953
BisI GCNGC 4 cut(s) 28, 163, 309, 346
BlsI GCNGC 4 cut(s) 29, 164, 310, 347
Bme1390I CCNGG 1 cut(s) 636
Bme18I GGWCC 4 cut(s) 653, 791, 987, 1019
BmeT110I CYCGRG 2 cut(s) 244, 657
BmgT120I GGNCC 4 cut(s) 653, 791, 987, 1019
BmiI GGNNCC 2 cut(s) 531, 792
BmrFI CCNGG 1 cut(s) 636
BpiI GAAGAC 1 cut(s) 63
BpmI CTGGAG 1 cut(s) 618
Bpu10I CCTNAGC 1 cut(s) 1278
BsaHI GRCGYC 1 cut(s) 303
BsaI GGTCTC 1 cut(s) 247
BsaJI CCNNGG 2 cut(s) 656, 892
BsaXI ACNNNNNCTCC 2 cut(s) 925, 955
Bsc4I CCNNNNNNNGG 3 cut(s) 256, 788, 952
Bse118I RCCGGY 1 cut(s) 781
Bse1I ACTGG 5 cut(s) 318, 439, 444, 778, 1352
Bse21I CCTNAGG 1 cut(s) 1230
BseBI CCWGG 1 cut(s) 636
BseDI CCNNGG 2 cut(s) 656, 892
BseGI GGATG 2 cut(s) 624, 1022
BseLI CCNNNNNNNGG 3 cut(s) 256, 788, 952
BseMII CTCAG 4 cut(s) 50, 345, 699, 1221
BseNI ACTGG 5 cut(s) 318, 439, 444, 778, 1352
BseRI GAGGAG 2 cut(s) 488, 491
BseXI GCAGC 3 cut(s) 149, 320, 357
BshFI GGCC 4 cut(s) 295, 460, 631, 777
BsiHKCI CYCGRG 2 cut(s) 244, 657
BsiSI CCGG 1 cut(s) 782
BslI CCNNNNNNNGG 3 cut(s) 256, 788, 952
BsmAI GTCTC 2 cut(s) 247, 691
BsmI GAATGC 1 cut(s) 1279
BsnI GGCC 4 cut(s) 295, 460, 631, 777
Bso31I GGTCTC 1 cut(s) 247
BsoBI CYCGRG 2 cut(s) 244, 657
Bsp1407I TGTACA 2 cut(s) 85, 736
Bsp143I GATC 6 cut(s) 67, 176, 316, 362, 730, 1264
BspACI CCGC 3 cut(s) 28, 194, 289
BspANI GGCC 4 cut(s) 295, 460, 631, 777
BspCNI CTCAG 4 cut(s) 49, 344, 698, 1222
BspHI TCATGA 2 cut(s) 144, 754
BspLI GGNNCC 2 cut(s) 531, 792
BspMAI CTGCAG 1 cut(s) 1261
BspPI GGATC 2 cut(s) 324, 370
BspTNI GGTCTC 1 cut(s) 247
BsrBI CCGCTC 1 cut(s) 289
BsrFI RCCGGY 1 cut(s) 781
BsrGI TGTACA 2 cut(s) 85, 736
BsrI ACTGG 5 cut(s) 318, 439, 444, 778, 1352
BssAI RCCGGY 1 cut(s) 781
BssECI CCNNGG 2 cut(s) 656, 892
BssMI GATC 6 cut(s) 67, 176, 316, 362, 730, 1264
BssNAI GTATAC 1 cut(s) 580
BssNI GRCGYC 1 cut(s) 303
BssT1I CCWWGG 1 cut(s) 892
Bst1107I GTATAC 1 cut(s) 580
Bst2UI CCWGG 1 cut(s) 636
Bst4CI ACNGT 6 cut(s) 410, 515, 679, 847, 925, 971
Bst6I CTCTTC 1 cut(s) 768
BstACI GRCGYC 1 cut(s) 303
BstAUI TGTACA 2 cut(s) 85, 736
BstC8I GCNNGC 3 cut(s) 155, 779, 1101
BstDEI CTNAG 5 cut(s) 36, 331, 685, 1230, 1278
BstF5I GGATG 2 cut(s) 624, 1022
BstHHI GCGC 1 cut(s) 350
BstKTI GATC 6 cut(s) 70, 179, 319, 365, 733, 1267
BstMAI GTCTC 2 cut(s) 247, 691
BstMBI GATC 6 cut(s) 67, 176, 316, 362, 730, 1264
BstMWI GCNNNNNNNGC 4 cut(s) 27, 159, 381, 1109
BstNI CCWGG 1 cut(s) 636
BstNSI RCATGY 1 cut(s) 704
BstSCI CCNGG 1 cut(s) 634
BstSFI CTRYAG 3 cut(s) 324, 489, 1257
BstV1I GCAGC 3 cut(s) 149, 320, 357
BstV2I GAAGAC 1 cut(s) 63
BstX2I RGATCY 2 cut(s) 316, 362
BstYI RGATCY 2 cut(s) 316, 362
BstZ17I GTATAC 1 cut(s) 580
Bsu36I CCTNAGG 1 cut(s) 1230
BsuI GTATCC 1 cut(s) 953
BsuRI GGCC 4 cut(s) 295, 460, 631, 777
BtsCI GGATG 2 cut(s) 624, 1022
BtsI GCAGTG 1 cut(s) 1254
BtsIMutI CAGTG 3 cut(s) 311, 520, 1254
Cac8I GCNNGC 3 cut(s) 155, 779, 1101
CciI TCATGA 2 cut(s) 144, 754
CfoI GCGC 1 cut(s) 350
Cfr10I RCCGGY 1 cut(s) 781
Cfr13I GGNCC 4 cut(s) 653, 791, 987, 1019
CseI GACGC 1 cut(s) 292
Csp6I GTAC 6 cut(s) 86, 265, 397, 564, 737, 848
CspCI CAANNNNNGTGG 2 cut(s) 339, 374
CviQI GTAC 6 cut(s) 86, 265, 397, 564, 737, 848
DdeI CTNAG 5 cut(s) 36, 331, 685, 1230, 1278
DpnI GATC 6 cut(s) 69, 178, 318, 364, 732, 1266
DpnII GATC 6 cut(s) 67, 176, 316, 362, 730, 1264
Eam1104I CTCTTC 1 cut(s) 768
EarI CTCTTC 1 cut(s) 768
Eco130I CCWWGG 1 cut(s) 892
Eco31I GGTCTC 1 cut(s) 247
Eco32I GATATC 2 cut(s) 115, 1152
Eco47I GGWCC 4 cut(s) 653, 791, 987, 1019
Eco57I CTGAAG 1 cut(s) 1121
Eco81I CCTNAGG 1 cut(s) 1230
Eco88I CYCGRG 2 cut(s) 244, 657
EcoO109I RGGNCCY 1 cut(s) 791
EcoRII CCWGG 1 cut(s) 634
EcoRV GATATC 2 cut(s) 115, 1152
EcoT14I CCWWGG 1 cut(s) 892
EcoT22I ATGCAT 1 cut(s) 811
ErhI CCWWGG 1 cut(s) 892
FauI CCCGC 1 cut(s) 201
FauNDI CATATG 1 cut(s) 419
FbaI TGATCA 2 cut(s) 67, 730
FblI GTMKAC 2 cut(s) 276, 579
Fnu4HI GCNGC 4 cut(s) 28, 163, 309, 346
FokI GGATG 2 cut(s) 631, 1009
Fsp4HI GCNGC 4 cut(s) 28, 163, 309, 346
FspBI CTAG 4 cut(s) 342, 470, 882, 939
GlaI GCGC 1 cut(s) 349
GluI GCNGC 4 cut(s) 28, 163, 309, 346
GsuI CTGGAG 1 cut(s) 618
HaeIII GGCC 4 cut(s) 295, 460, 631, 777
HapII CCGG 1 cut(s) 782
HgaI GACGC 1 cut(s) 292
HhaI GCGC 1 cut(s) 350
Hin1I GRCGYC 1 cut(s) 303
Hin6I GCGC 1 cut(s) 348
HinP1I GCGC 1 cut(s) 348
HincII GTYRAC 3 cut(s) 277, 483, 1165
HindII GTYRAC 3 cut(s) 277, 483, 1165
HindIII AAGCTT 1 cut(s) 1189
HpaI GTTAAC 1 cut(s) 483
HpaII CCGG 1 cut(s) 782
HphI GGTGA 5 cut(s) 449, 650, 676, 967, 1053
Hpy166II GTNNAC 7 cut(s) 277, 415, 483, 498, 580, 682, 1165
Hpy188I TCNGA 7 cut(s) 39, 72, 259, 688, 836, 868, 1224
Hpy188III TCNNGA 9 cut(s) 136, 145, 219, 244, 470, 606, 659, 755, 901
Hpy8I GTNNAC 7 cut(s) 277, 415, 483, 498, 580, 682, 1165
Hpy99I CGWCG 2 cut(s) 65, 308
HpyAV CCTTC 2 cut(s) 46, 816
HpyCH4III ACNGT 6 cut(s) 410, 515, 679, 847, 925, 971
HpyCH4IV ACGT 1 cut(s) 816
HpyF10VI GCNNNNNNNGC 4 cut(s) 27, 159, 381, 1109
HpyF3I CTNAG 5 cut(s) 36, 331, 685, 1230, 1278
HpySE526I ACGT 1 cut(s) 816
Hsp92I GRCGYC 1 cut(s) 303
HspAI GCGC 1 cut(s) 348
Ksp22I TGATCA 2 cut(s) 67, 730
KspAI GTTAAC 1 cut(s) 483
Kzo9I GATC 6 cut(s) 67, 176, 316, 362, 730, 1264
LmnI GCTCC 1 cut(s) 529
Lsp1109I GCAGC 3 cut(s) 149, 320, 357
MaeI CTAG 4 cut(s) 342, 470, 882, 939
MaeII ACGT 1 cut(s) 816
MaeIII GTNAC 2 cut(s) 1037, 1342
MalI GATC 6 cut(s) 69, 178, 318, 364, 732, 1266
MbiI CCGCTC 1 cut(s) 289
MboI GATC 6 cut(s) 67, 176, 316, 362, 730, 1264
MboII GAAGA 7 cut(s) 16, 68, 218, 755, 855, 1149, 1295
MfeI CAATTG 1 cut(s) 89
MflI RGATCY 2 cut(s) 316, 362
MlyI GAGTC 5 cut(s) 323, 331, 503, 677, 974
MmeI TCCRAC 4 cut(s) 674, 799, 836, 911
Mph1103I ATGCAT 1 cut(s) 811
MseI TTAA 4 cut(s) 482, 1203, 1319, 1360
MslI CAYNNNNRTG 5 cut(s) 738, 969, 1007, 1066, 1272
MspI CCGG 1 cut(s) 782
MspR9I CCNGG 1 cut(s) 636
MunI CAATTG 1 cut(s) 89
Mva1269I GAATGC 1 cut(s) 1279
MvaI CCWGG 1 cut(s) 636
MwoI GCNNNNNNNGC 4 cut(s) 27, 159, 381, 1109
NdeI CATATG 1 cut(s) 419
NdeII GATC 6 cut(s) 67, 176, 316, 362, 730, 1264
NlaIV GGNNCC 2 cut(s) 531, 792
NsiI ATGCAT 1 cut(s) 811
NspI RCATGY 1 cut(s) 704
PaeR7I CTCGAG 1 cut(s) 244
PagI TCATGA 2 cut(s) 144, 754
PciI ACATGT 1 cut(s) 700
PcsI WCGNNNNNNNCGW 1 cut(s) 282
PctI GAATGC 1 cut(s) 1279
PfeI GAWTC 6 cut(s) 440, 546, 625, 758, 885, 897
PkrI GCNGC 4 cut(s) 29, 164, 310, 347
PleI GAGTC 5 cut(s) 323, 331, 502, 677, 974
PpsI GAGTC 5 cut(s) 323, 331, 502, 677, 974
PpuMI RGGWCCY 1 cut(s) 791
PscI ACATGT 1 cut(s) 700
Psp5II RGGWCCY 1 cut(s) 791
Psp6I CCWGG 1 cut(s) 634
PspGI CCWGG 1 cut(s) 634
PspN4I GGNNCC 2 cut(s) 531, 792
PspPI GGNCC 4 cut(s) 653, 791, 987, 1019
PspPPI RGGWCCY 1 cut(s) 791
PstI CTGCAG 1 cut(s) 1261
PsuI RGATCY 2 cut(s) 316, 362
RsaI GTAC 6 cut(s) 87, 266, 398, 565, 738, 849
RsaNI GTAC 6 cut(s) 86, 265, 397, 564, 737, 848
RseI CAYNNNNRTG 5 cut(s) 738, 969, 1007, 1066, 1272
SalI GTCGAC 1 cut(s) 275
SaqAI TTAA 4 cut(s) 482, 1203, 1319, 1360
SatI GCNGC 4 cut(s) 28, 163, 309, 346
Sau3AI GATC 6 cut(s) 67, 176, 316, 362, 730, 1264
Sau96I GGNCC 4 cut(s) 653, 791, 987, 1019
SchI GAGTC 5 cut(s) 323, 331, 503, 677, 974
ScrFI CCNGG 1 cut(s) 636
SfcI CTRYAG 3 cut(s) 324, 489, 1257
Sfr274I CTCGAG 1 cut(s) 244
SinI GGWCC 4 cut(s) 653, 791, 987, 1019
SlaI CTCGAG 1 cut(s) 244
SmiMI CAYNNNNRTG 5 cut(s) 738, 969, 1007, 1066, 1272
SmlI CTYRAG 1 cut(s) 244
SmoI CTYRAG 1 cut(s) 244
SsiI CCGC 3 cut(s) 28, 194, 289
SspMI CTAG 4 cut(s) 342, 470, 882, 939
StyD4I CCNGG 1 cut(s) 634
StyI CCWWGG 1 cut(s) 892
TaaI ACNGT 6 cut(s) 410, 515, 679, 847, 925, 971
TaiI ACGT 1 cut(s) 819
TaqI TCGA 5 cut(s) 220, 245, 276, 975, 1356
TatI WGTACW 3 cut(s) 85, 396, 736
TauI GCSGC 1 cut(s) 30
TfiI GAWTC 6 cut(s) 440, 546, 625, 758, 885, 897
Tru1I TTAA 4 cut(s) 482, 1203, 1319, 1360
Tru9I TTAA 4 cut(s) 482, 1203, 1319, 1360
TscAI CASTG 3 cut(s) 318, 520, 1261
TseI GCWGC 3 cut(s) 162, 308, 345
TspDTI ATGAA 6 cut(s) 17, 161, 734, 743, 755, 974
TspRI CASTG 3 cut(s) 318, 520, 1261
VpaK11BI GGWCC 4 cut(s) 653, 791, 987, 1019
XapI RAATTY 4 cut(s) 522, 710, 1047, 1294
XbaI TCTAGA 1 cut(s) 469
XceI RCATGY 1 cut(s) 704
XhoI CTCGAG 1 cut(s) 244
XmiI GTMKAC 2 cut(s) 276, 579
XspI CTAG 4 cut(s) 342, 470, 882, 939
Zsp2I ATGCAT 1 cut(s) 811
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.