Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0012585

Physical Location & Seq

Genomic Coordinates Forward (+)

29738439 .. 29743422

Gene Structure 4984 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G012640.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1836 bp

ATGAAGAATACCGAACTATGCCATAGCCGCCGACCTCAGAAGCAACATGAGAAGGAAGACGACGATGATCAGAAACCCTACCTTGTACAATTGCCAGACCATATAATTGTCGATATCTCCTGCAAAATCCCAATCAAGACCCTCATGAAATGCAGGCATGTCTGCAAATCTTTGCGATCTTTGTTCGCAGACCCGCAATTTACCGAAGAACTATTCTCTCGAACACGCCCTTGCCATTTGGTCTCGAGCCTCAATTCGGATAGGTACTTCTTGGTCGACTTCAACGAGCGGAGGCCAAACGGCGTCGCAGCACTGGATCTTTCTATAGACTCAGCGACTCCTAGCAGCGCAATCATCGTGGGATCTTGCAAGGGCATGGTCTGCTTATACAAGATGTACTGCCCTTACGGTGTTTACATATGCAATCCAATGACTGGTGATTCCAGTTTCCTTTATTGGCCTTTATCTCTAGAGGAGGAGTTAACTCCTGTACAGTCCACTCCTACAAGACACATTGAGGAAATTTTGGAGCCATCTTTTTGTGGATTTGGGTTCGGATATAGTACCATAAATGGTGTCTACAAAATTGTTCTTATTGCTTATTCAAGAAAAATGAGGATGGGGATTCGGCCTTCAGGTGAGATTGCGTATGGACCTCGGGAGGTGATGGTTTTGACTGTGGACTCAGGGGGTTGGAGACATGTTGGGGAATTTGATATGAACAATTTTGATCATGTACATGGAGTTTATATTCATGACTGTCTTCATTGGACTGGCCTACCGGTAAAGGGTCCTCGTTTGATATATGCATTTGATGTTGGAAGTGAGTGTTTCCGAGAGCTACCAGTACCTCGTTGGACTTCTTTGGAGAGCCAAATGACTAGATTCAACCTTGGAATCTTGAATGATTGCCTCTCTATAACTGTTTGTTGGAGAGCTAGTATCCATGTTTGGGTGATGAAGCATTACGGTGTTGAGAACTCTTGGACCAAAGAGCTTGACATTAGTGTGGAATATGGTCCGTCGTCGTTTGGTGGTTACGCACAAATTTTGAGTTTTTCACCAGAATGGCAAGTCCTGTTGTGGCAGGGTAGGTTGCAGGCTCATACTGCTGGAAGTGAGGGCTTTGTTGGGGTTCAGGTTGATGGGATACCATCTTCGGTTGACAAAGCCTATCCCCATGTTCCAAGCTTTATTTCACTTAAAGATGTTATCAGGTGTCAGCACCCTGAGGGGCAAGGAAATGTGCAAACCACTGCAGTAGATCATGGGAATGCTAAGGAAGATAAACAGAATTTGCAATCTAATGCTCATGGATTAAATCAAGGGAATGTGCAATCTGTAACTGTACCAGTAGATCAACAGCAACAGGGCCAACTGAATGTGCAATCCAATGCAGCAGATCATGGGAATGCTGAAGCAGATAAAGGGAATGTGCAATTTAATGCTGATCAATTAGATCAAGGGAATTTGCAATCTATAGCTGTAACAATTACCCAAGAAAGACAGGAGAATAGAGCACCCATGAAGGCAAAAACCACACATGAAGTTCAACCAATTAATGCAAGCCTCTGCATCCCAACCATTTTATGCATCACCACCCAAGCCAACACAGCCATCTGTTGTGCAGCTATGAACTGCAAACCAGTTCATTCATCCCACAACTGCGATAAAGATGAAGACCAAGTCCCCTGCAAAGAGGAAGAGTGCTCCAGCAGAGAAGTAACTGAGTCCCCTGCTAAGATGAAGCATTTTGGGTGTAATTTCAGATATTTCAAAGCATTGGGCTTTTGTTCACTGTATAACAAATCATTTGTATCGGAACTTGGTAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box FBA_1

Protein Analysis

611

Amino Acids

68.39

Weight (kDa)

5.97

Isoelectric Point (pI)

45.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box	PF00646	30 - 68	1.5e-06	F-box domain
FBA_3	PF08268	112 - 332	2.9e-10	F-box associated beta propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012585)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g40010
rosa_chinensis	RchiOBHm_Chr4g0409251 RchiOBHm_Chr6g0300741
rosa_laevigata	RLG00000008583 RLG00000011306
rosa_multiflora	Rmu_sc0001569.1_g000004 Rmu_sc0006723.1_g000005 Rmu_sc0032596.1_g000001
rosa_roxburghii	Rroxscaffold_5G00353720 Rroxscaffold_7G00167460
rosa_rugosa	Rorug04G0091500 Rorug04G0091500
rosa_samantha	Rh4AG154900 Rh4BG152500 Rh4BG175800 Rh4CG163900 Rh4DG147700 Rh6AG411600
rosa_wichuraiana	Rw4G012640

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	434
AccBSI	CCGCTC	1 cut(s)	289
AccI	GTMKAC	2 cut(s)	276, 579
AciI	CCGC	3 cut(s)	28, 194, 289
AclWI	GGATC	2 cut(s)	324, 370
AcsI	RAATTY	5 cut(s)	522, 710, 1047, 1294, 1468
AcuI	CTGAAG	2 cut(s)	618, 1437
AcyI	GRCGYC	1 cut(s)	303
AfaI	GTAC	8 cut(s)	87, 266, 398, 492, 565, 738, 849, 1350
AfiI	CCNNNNNNNGG	4 cut(s)	256, 434, 788, 952
AflIII	ACRYGT	1 cut(s)	700
AgeI	ACCGGT	1 cut(s)	781
AgsI	TTSAA	6 cut(s)	283, 606, 889, 904, 1553, 1777
AjuI	GAANNNNNNNTTGG	2 cut(s)	1180, 1212
AluBI	AGCT	6 cut(s)	841, 938, 997, 1191, 1484, 1631
AluI	AGCT	6 cut(s)	841, 938, 997, 1191, 1484, 1631
Alw21I	GWGCWC	2 cut(s)	1522, 1712
Alw26I	GTCTC	2 cut(s)	247, 691
AlwI	GGATC	2 cut(s)	324, 370
Ama87I	CYCGRG	2 cut(s)	244, 657
AoxI	GGCC	5 cut(s)	293, 458, 629, 775, 1372
ApeKI	GCWGC	4 cut(s)	308, 345, 1397, 1628
ApoI	RAATTY	5 cut(s)	522, 710, 1047, 1294, 1468
AseI	ATTAAT	1 cut(s)	1560
AsiGI	ACCGGT	1 cut(s)	781
AspLEI	GCGC	1 cut(s)	350
AspS9I	GGNCC	5 cut(s)	653, 791, 987, 1019, 1372
AsuHPI	GGTGA	6 cut(s)	449, 650, 676, 967, 1053, 1588
AvaI	CYCGRG	2 cut(s)	244, 657
AvaII	GGWCC	4 cut(s)	653, 791, 987, 1019
AxyI	CCTNAGG	1 cut(s)	1230
BbsI	GAAGAC	3 cut(s)	63, 755, 1686
Bbv12I	GWGCWC	2 cut(s)	1522, 1712
BbvI	GCAGC	4 cut(s)	320, 357, 1409, 1640
BccI	CCATC	6 cut(s)	541, 613, 661, 1139, 1162, 1625
BceAI	ACGGC	1 cut(s)	316
BciVI	GTATCC	2 cut(s)	953, 1143
BclI	TGATCA	3 cut(s)	67, 730, 1450
BcoDI	GTCTC	2 cut(s)	247, 691
BfaI	CTAG	4 cut(s)	342, 470, 882, 939
BfmI	CTRYAG	3 cut(s)	324, 1257, 1479
BfuI	GTATCC	2 cut(s)	953, 1143
BisI	GCNGC	5 cut(s)	28, 309, 346, 1398, 1629
BlsI	GCNGC	5 cut(s)	29, 310, 347, 1399, 1630
Bme18I	GGWCC	4 cut(s)	653, 791, 987, 1019
BmeT110I	CYCGRG	2 cut(s)	244, 657
BmgT120I	GGNCC	5 cut(s)	653, 791, 987, 1019, 1372
BmiI	GGNNCC	2 cut(s)	531, 792
BmsI	GCATC	2 cut(s)	1584, 1602
BpiI	GAAGAC	3 cut(s)	63, 755, 1686
BpmI	CTGGAG	1 cut(s)	1696
Bpu10I	CCTNAGC	1 cut(s)	1278
BsaHI	GRCGYC	1 cut(s)	303
BsaI	GGTCTC	1 cut(s)	247
BsaJI	CCNNGG	2 cut(s)	656, 892
BsaWI	WCCGGW	1 cut(s)	781
BsaXI	ACNNNNNCTCC	2 cut(s)	925, 955
Bsc4I	CCNNNNNNNGG	4 cut(s)	256, 434, 788, 952
Bse118I	RCCGGY	1 cut(s)	781
Bse1I	ACTGG	7 cut(s)	318, 439, 444, 778, 845, 1352, 1646
Bse21I	CCTNAGG	1 cut(s)	1230
BseDI	CCNNGG	2 cut(s)	656, 892
BseGI	GGATG	3 cut(s)	624, 1575, 1655
BseLI	CCNNNNNNNGG	4 cut(s)	256, 434, 788, 952
BseMII	CTCAG	5 cut(s)	50, 345, 699, 1221, 1719
BseNI	ACTGG	7 cut(s)	318, 439, 444, 778, 845, 1352, 1646
BseRI	GAGGAG	2 cut(s)	488, 491
BseXI	GCAGC	4 cut(s)	320, 357, 1409, 1640
BsgI	GTGCAG	1 cut(s)	1647
BshFI	GGCC	5 cut(s)	295, 460, 631, 777, 1374
BshTI	ACCGGT	1 cut(s)	781
BsiHKAI	GWGCWC	2 cut(s)	1522, 1712
BsiHKCI	CYCGRG	2 cut(s)	244, 657
BsiSI	CCGG	1 cut(s)	782
BslFI	GGGAC	2 cut(s)	1673, 1717
BslI	CCNNNNNNNGG	4 cut(s)	256, 434, 788, 952
BsmAI	GTCTC	2 cut(s)	247, 691
BsmFI	GGGAC	2 cut(s)	1673, 1717
BsmI	GAATGC	2 cut(s)	1279, 1417
BsnI	GGCC	5 cut(s)	295, 460, 631, 777, 1374
Bso31I	GGTCTC	1 cut(s)	247
BsoBI	CYCGRG	2 cut(s)	244, 657
Bsp1286I	GDGCHC	2 cut(s)	1522, 1712
Bsp1407I	TGTACA	3 cut(s)	85, 490, 736
BspACI	CCGC	3 cut(s)	28, 194, 289
BspANI	GGCC	5 cut(s)	295, 460, 631, 777, 1374
BspCNI	CTCAG	5 cut(s)	49, 344, 698, 1222, 1720
BspHI	TCATGA	2 cut(s)	144, 754
BspLI	GGNNCC	2 cut(s)	531, 792
BspMAI	CTGCAG	1 cut(s)	1261
BspPI	GGATC	2 cut(s)	324, 370
BspTNI	GGTCTC	1 cut(s)	247
BsrBI	CCGCTC	1 cut(s)	289
BsrFI	RCCGGY	1 cut(s)	781
BsrGI	TGTACA	3 cut(s)	85, 490, 736
BsrI	ACTGG	7 cut(s)	318, 439, 444, 778, 845, 1352, 1646
BssAI	RCCGGY	1 cut(s)	781
BssECI	CCNNGG	2 cut(s)	656, 892
BssNI	GRCGYC	1 cut(s)	303
BssT1I	CCWWGG	1 cut(s)	892
Bst4CI	ACNGT	8 cut(s)	410, 495, 679, 761, 925, 971, 1348, 1800
Bst6I	CTCTTC	1 cut(s)	1698
BstACI	GRCGYC	1 cut(s)	303
BstAPI	GCANNNNNTGC	1 cut(s)	381
BstAUI	TGTACA	3 cut(s)	85, 490, 736
BstC8I	GCNNGC	3 cut(s)	155, 1101, 1567
BstDEI	CTNAG	7 cut(s)	36, 331, 685, 1230, 1278, 1728, 1740
BstF5I	GGATG	3 cut(s)	624, 1575, 1655
BstHHI	GCGC	1 cut(s)	350
BstMAI	GTCTC	2 cut(s)	247, 691
BstMWI	GCNNNNNNNGC	4 cut(s)	27, 381, 1109, 1613
BstNSI	RCATGY	2 cut(s)	161, 704
BstSFI	CTRYAG	3 cut(s)	324, 1257, 1479
BstV1I	GCAGC	4 cut(s)	320, 357, 1409, 1640
BstV2I	GAAGAC	3 cut(s)	63, 755, 1686
BstX2I	RGATCY	2 cut(s)	316, 362
BstYI	RGATCY	2 cut(s)	316, 362
Bsu36I	CCTNAGG	1 cut(s)	1230
BsuI	GTATCC	2 cut(s)	953, 1143
BsuRI	GGCC	5 cut(s)	295, 460, 631, 777, 1374
BtsCI	GGATG	3 cut(s)	624, 1575, 1655
BtsI	GCAGTG	1 cut(s)	1254
BtsIMutI	CAGTG	3 cut(s)	311, 1254, 1796
Cac8I	GCNNGC	3 cut(s)	155, 1101, 1567
CciI	TCATGA	2 cut(s)	144, 754
CfoI	GCGC	1 cut(s)	350
Cfr10I	RCCGGY	1 cut(s)	781
Cfr13I	GGNCC	5 cut(s)	653, 791, 987, 1019, 1372
CseI	GACGC	1 cut(s)	292
Csp6I	GTAC	8 cut(s)	86, 265, 397, 491, 564, 737, 848, 1349
CspAI	ACCGGT	1 cut(s)	781
CspCI	CAANNNNNGTGG	2 cut(s)	339, 374
CviQI	GTAC	8 cut(s)	86, 265, 397, 491, 564, 737, 848, 1349
DdeI	CTNAG	7 cut(s)	36, 331, 685, 1230, 1278, 1728, 1740
Eam1104I	CTCTTC	1 cut(s)	1698
EarI	CTCTTC	1 cut(s)	1698
Eco130I	CCWWGG	1 cut(s)	892
Eco31I	GGTCTC	1 cut(s)	247
Eco32I	GATATC	1 cut(s)	115
Eco47I	GGWCC	4 cut(s)	653, 791, 987, 1019
Eco57I	CTGAAG	2 cut(s)	618, 1437
Eco81I	CCTNAGG	1 cut(s)	1230
Eco88I	CYCGRG	2 cut(s)	244, 657
EcoO109I	RGGNCCY	1 cut(s)	791
EcoRV	GATATC	1 cut(s)	115
EcoT14I	CCWWGG	1 cut(s)	892
EcoT22I	ATGCAT	2 cut(s)	811, 1595
ErhI	CCWWGG	1 cut(s)	892
FaqI	GGGAC	2 cut(s)	1673, 1717
FauI	CCCGC	1 cut(s)	201
FauNDI	CATATG	1 cut(s)	419
FbaI	TGATCA	3 cut(s)	67, 730, 1450
FblI	GTMKAC	2 cut(s)	276, 579
Fnu4HI	GCNGC	5 cut(s)	28, 309, 346, 1398, 1629
FokI	GGATG	3 cut(s)	631, 1562, 1642
Fsp4HI	GCNGC	5 cut(s)	28, 309, 346, 1398, 1629
FspBI	CTAG	4 cut(s)	342, 470, 882, 939
GlaI	GCGC	1 cut(s)	349
GluI	GCNGC	5 cut(s)	28, 309, 346, 1398, 1629
GsuI	CTGGAG	1 cut(s)	1696
HaeIII	GGCC	5 cut(s)	295, 460, 631, 777, 1374
HapII	CCGG	1 cut(s)	782
HgaI	GACGC	1 cut(s)	292
HhaI	GCGC	1 cut(s)	350
Hin1I	GRCGYC	1 cut(s)	303
Hin6I	GCGC	1 cut(s)	348
HinP1I	GCGC	1 cut(s)	348
HincII	GTYRAC	3 cut(s)	277, 483, 1165
HindII	GTYRAC	3 cut(s)	277, 483, 1165
HindIII	AAGCTT	1 cut(s)	1189
HinfI	GANTC	8 cut(s)	329, 337, 440, 625, 683, 885, 897, 1730
HpaI	GTTAAC	1 cut(s)	483
HpaII	CCGG	1 cut(s)	782
HphI	GGTGA	6 cut(s)	449, 650, 676, 967, 1053, 1588
Hpy166II	GTNNAC	8 cut(s)	277, 415, 483, 498, 580, 682, 1165, 1796
Hpy188I	TCNGA	7 cut(s)	39, 72, 259, 557, 836, 1769, 1822
Hpy188III	TCNNGA	9 cut(s)	136, 145, 219, 244, 470, 606, 659, 755, 901
Hpy8I	GTNNAC	8 cut(s)	277, 415, 483, 498, 580, 682, 1165, 1796
Hpy99I	CGWCG	4 cut(s)	65, 308, 1027, 1030
HpyAV	CCTTC	3 cut(s)	46, 642, 1522
HpyCH4III	ACNGT	8 cut(s)	410, 495, 679, 761, 925, 971, 1348, 1800
HpyF10VI	GCNNNNNNNGC	4 cut(s)	27, 381, 1109, 1613
HpyF3I	CTNAG	7 cut(s)	36, 331, 685, 1230, 1278, 1728, 1740
Hsp92I	GRCGYC	1 cut(s)	303
HspAI	GCGC	1 cut(s)	348
Ksp22I	TGATCA	3 cut(s)	67, 730, 1450
KspAI	GTTAAC	1 cut(s)	483
LmnI	GCTCC	2 cut(s)	529, 1715
Lsp1109I	GCAGC	4 cut(s)	320, 357, 1409, 1640
LweI	GCATC	2 cut(s)	1584, 1602
MaeI	CTAG	4 cut(s)	342, 470, 882, 939
MaeIII	GTNAC	5 cut(s)	1037, 1342, 1486, 1723, 1829
MbiI	CCGCTC	1 cut(s)	289
MboII	GAAGA	8 cut(s)	16, 68, 218, 755, 1149, 1295, 1691, 1715
MfeI	CAATTG	1 cut(s)	89
MflI	RGATCY	2 cut(s)	316, 362
MhlI	GDGCHC	2 cut(s)	1522, 1712
MlyI	GAGTC	4 cut(s)	323, 331, 677, 1739
MmeI	TCCRAC	4 cut(s)	674, 799, 836, 911
Mph1103I	ATGCAT	2 cut(s)	811, 1595
MseI	TTAA	5 cut(s)	482, 1203, 1319, 1443, 1560
MslI	CAYNNNNRTG	6 cut(s)	738, 969, 1007, 1066, 1272, 1410
MspI	CCGG	1 cut(s)	782
MunI	CAATTG	1 cut(s)	89
Mva1269I	GAATGC	2 cut(s)	1279, 1417
MwoI	GCNNNNNNNGC	4 cut(s)	27, 381, 1109, 1613
NdeI	CATATG	1 cut(s)	419
NlaIV	GGNNCC	2 cut(s)	531, 792
NsiI	ATGCAT	2 cut(s)	811, 1595
NspI	RCATGY	2 cut(s)	161, 704
PaeR7I	CTCGAG	1 cut(s)	244
PagI	TCATGA	2 cut(s)	144, 754
PciI	ACATGT	1 cut(s)	700
PcsI	WCGNNNNNNNCGW	1 cut(s)	282
PctI	GAATGC	2 cut(s)	1279, 1417
PfeI	GAWTC	4 cut(s)	440, 625, 885, 897
PflFI	GACNNNGTC	1 cut(s)	1685
PflMI	CCANNNNNTGG	1 cut(s)	434
PinAI	ACCGGT	1 cut(s)	781
PkrI	GCNGC	5 cut(s)	29, 310, 347, 1399, 1630
PleI	GAGTC	4 cut(s)	323, 331, 677, 1738
PpsI	GAGTC	4 cut(s)	323, 331, 677, 1738
PpuMI	RGGWCCY	1 cut(s)	791
PscI	ACATGT	1 cut(s)	700
PshBI	ATTAAT	1 cut(s)	1560
Psp5II	RGGWCCY	1 cut(s)	791
PspN4I	GGNNCC	2 cut(s)	531, 792
PspPI	GGNCC	5 cut(s)	653, 791, 987, 1019, 1372
PspPPI	RGGWCCY	1 cut(s)	791
PstI	CTGCAG	1 cut(s)	1261
PsuI	RGATCY	2 cut(s)	316, 362
PsyI	GACNNNGTC	1 cut(s)	1685
RsaI	GTAC	8 cut(s)	87, 266, 398, 492, 565, 738, 849, 1350
RsaNI	GTAC	8 cut(s)	86, 265, 397, 491, 564, 737, 848, 1349
RseI	CAYNNNNRTG	6 cut(s)	738, 969, 1007, 1066, 1272, 1410
SalI	GTCGAC	1 cut(s)	275
SaqAI	TTAA	5 cut(s)	482, 1203, 1319, 1443, 1560
SatI	GCNGC	5 cut(s)	28, 309, 346, 1398, 1629
Sau96I	GGNCC	5 cut(s)	653, 791, 987, 1019, 1372
SchI	GAGTC	4 cut(s)	323, 331, 677, 1739
SduI	GDGCHC	2 cut(s)	1522, 1712
SfaNI	GCATC	2 cut(s)	1584, 1602
SfcI	CTRYAG	3 cut(s)	324, 1257, 1479
Sfr274I	CTCGAG	1 cut(s)	244
SinI	GGWCC	4 cut(s)	653, 791, 987, 1019
SlaI	CTCGAG	1 cut(s)	244
SmiMI	CAYNNNNRTG	6 cut(s)	738, 969, 1007, 1066, 1272, 1410
SmlI	CTYRAG	1 cut(s)	244
SmoI	CTYRAG	1 cut(s)	244
SsiI	CCGC	3 cut(s)	28, 194, 289
SspMI	CTAG	4 cut(s)	342, 470, 882, 939
StyI	CCWWGG	1 cut(s)	892
TaaI	ACNGT	8 cut(s)	410, 495, 679, 761, 925, 971, 1348, 1800
TaqI	TCGA	4 cut(s)	111, 220, 245, 276
TatI	WGTACW	4 cut(s)	85, 396, 490, 736
TauI	GCSGC	1 cut(s)	30
TfiI	GAWTC	4 cut(s)	440, 625, 885, 897
Tru1I	TTAA	5 cut(s)	482, 1203, 1319, 1443, 1560
Tru9I	TTAA	5 cut(s)	482, 1203, 1319, 1443, 1560
TscAI	CASTG	3 cut(s)	318, 1261, 1803
TseI	GCWGC	4 cut(s)	308, 345, 1397, 1628
TspGWI	ACGGA	1 cut(s)	1011
TspRI	CASTG	3 cut(s)	318, 1261, 1803
Tth111I	GACNNNGTC	1 cut(s)	1685
Van91I	CCANNNNNTGG	1 cut(s)	434
VpaK11BI	GGWCC	4 cut(s)	653, 791, 987, 1019
VspI	ATTAAT	1 cut(s)	1560
XapI	RAATTY	5 cut(s)	522, 710, 1047, 1294, 1468
XbaI	TCTAGA	1 cut(s)	469
XceI	RCATGY	2 cut(s)	161, 704
XcmI	CCANNNNNNNNNTGG	1 cut(s)	852
XhoI	CTCGAG	1 cut(s)	244
XmiI	GTMKAC	2 cut(s)	276, 579
XspI	CTAG	4 cut(s)	342, 470, 882, 939
Zsp2I	ATGCAT	2 cut(s)	811, 1595

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.