RLG00000008583

F-box protein At3g07870-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0012585
Physical Location & Seq
Reverse (-)
32532058 .. 32536225
4168 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000008583

Sequence Viewer

Length: 1191 bp
ATGCAATCCAATGACTCTTCATCTCTTCCACCTCCTATTAGGGAAGTTGATTCCATTTTCCTTTATTGGCCTTTGTCTCTTGAGGAGGAGTTAGCTCCTGTAGAGTCCACTCCTACAAGACACATTGAGGAAATTTTGGAGCCATCTTTTTGTGGATTTGGGTTGGGATATAGTACCATAAGTGATGTATACAAAATTGTTCTTATTGCTTATTCAAGAAAAATGAGGATGGGGATTCGGCCTCCAGGTGAGATTGCGTATGGACCTCGGGAGGTGATGGTTTTGACTGTGGACTCAGAGGGTTGGAGACATGTTGGGGAATTTGATATGAACAGTTTTGATCATGTACATGGAGTTTATATTCATGATTGTCTTCATTGGACTGGCCTGCCGGTAAAGGGTCCTCGATTGATACATGCATTTGACGTTGGAAGGGAGTGTTTCCGAGAGCTACCAGTACCTTGTTGGACTTCTTCGGAGAGCCAAATGACTAGATTCAACCTTGGAATCTTGAATGATTGCCTCTCTATAACTGTTTGTTGGAGAGCTAGTATCCATGTTTGGGTGATGAAGCATTACGGTGTCGAGGACTCTTGGACCAAAGAGCTTGACATTAGTGTGGAATATGGTCCGTCGTCGTTTGGTGGTTACGCACAAATTTTGAGTTTTTCACCAGAATGGCAAGTCCTGTTGTGGCAGGGTAGGGTGCAGGCTCATACTGCTGGAAGTGAGGGCTTTGTTGGGGTTCAGGTTGATGGGATACCATCTTTGGTTGACAAAGCCTATCCCCATGGGCAAGGAAATGTGCAAACCAATGCAGTAGATCATGGGAATGCAAAGGAAGATAAACAGAATTTGCAATCTAATGCTTATGGATTAAATCAAGGGAATGTGCAATCTGTAACTGTACCAGTCGATCAACAGCAACAGGGCCAACTGAATGTGCAATCCAATGCAGCAGATCATGGGAGTGCTGAAGCAGATAAAGGGAATGTGCAATCTAATGCTGATCAATTAGATCAAGGGATTTTGCAATCTATAGATGCAACAATTGCCCAAGAAACACAGGATAATAGAGCAGCCAAGAAGGCAAAAACCACACATGAAGTTCAACCAATGCAAGCCTCTGCATCCCAACCATTTTATGCATCACCACCCAAGCCAACACAGCCATCTGTTGTGCAGCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_1

Protein Analysis

397

Amino Acids

43.77

Weight (kDa)

5.07

Isoelectric Point (pI)

47.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 55 - 202 1.7e-06 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 189
AcsI RAATTY 4 cut(s) 132, 320, 657, 853
AcuI CTGAAG 1 cut(s) 996
AfaI GTAC 4 cut(s) 175, 348, 459, 909
AfiI CCNNNNNNNGG 2 cut(s) 398, 562
AflIII ACRYGT 1 cut(s) 310
AgsI TTSAA 4 cut(s) 216, 499, 514, 1112
AjnI CCWGG 1 cut(s) 244
AluBI AGCT 5 cut(s) 95, 451, 548, 607, 1186
AluI AGCT 5 cut(s) 95, 451, 548, 607, 1186
Alw26I GTCTC 2 cut(s) 81, 301
Ama87I CYCGRG 1 cut(s) 267
AoxI GGCC 4 cut(s) 68, 239, 385, 931
ApeKI GCWGC 3 cut(s) 956, 1079, 1183
ApoI RAATTY 4 cut(s) 132, 320, 657, 853
AspS9I GGNCC 5 cut(s) 263, 401, 597, 629, 931
AsuHPI GGTGA 5 cut(s) 260, 286, 577, 663, 1143
AvaI CYCGRG 1 cut(s) 267
AvaII GGWCC 4 cut(s) 263, 401, 597, 629
BbsI GAAGAC 1 cut(s) 365
BbvI GCAGC 2 cut(s) 968, 1091
BccI CCATC 6 cut(s) 151, 223, 271, 749, 772, 1180
BciT130I CCWGG 1 cut(s) 246
BciVI GTATCC 2 cut(s) 563, 753
BclI TGATCA 2 cut(s) 340, 1009
BcoDI GTCTC 2 cut(s) 81, 301
BfaI CTAG 2 cut(s) 492, 549
BfmI CTRYAG 2 cut(s) 99, 1038
BfuI GTATCC 2 cut(s) 563, 753
BglI GCCNNNNNGGC 1 cut(s) 1088
BisI GCNGC 3 cut(s) 957, 1080, 1184
BlsI GCNGC 3 cut(s) 958, 1081, 1185
Bme1390I CCNGG 1 cut(s) 246
Bme18I GGWCC 4 cut(s) 263, 401, 597, 629
BmeT110I CYCGRG 1 cut(s) 267
BmgT120I GGNCC 5 cut(s) 263, 401, 597, 629, 931
BmiI GGNNCC 2 cut(s) 141, 402
BmrFI CCNGG 1 cut(s) 246
BmsI GCATC 3 cut(s) 1033, 1139, 1157
BpiI GAAGAC 1 cut(s) 365
BpmI CTGGAG 1 cut(s) 228
BpuEI CTTGAG 1 cut(s) 101
BsaJI CCNNGG 3 cut(s) 266, 502, 790
BsaXI ACNNNNNCTCC 2 cut(s) 535, 565
Bsc4I CCNNNNNNNGG 2 cut(s) 398, 562
Bse118I RCCGGY 1 cut(s) 391
Bse1I ACTGG 3 cut(s) 388, 455, 911
BseBI CCWGG 1 cut(s) 246
BseDI CCNNGG 3 cut(s) 266, 502, 790
BseGI GGATG 2 cut(s) 234, 1130
BseLI CCNNNNNNNGG 2 cut(s) 398, 562
BseMII CTCAG 1 cut(s) 309
BseNI ACTGG 3 cut(s) 388, 455, 911
BseRI GAGGAG 2 cut(s) 98, 101
BseXI GCAGC 2 cut(s) 968, 1091
BsgI GTGCAG 1 cut(s) 728
BshFI GGCC 4 cut(s) 70, 241, 387, 933
BsiHKCI CYCGRG 1 cut(s) 267
BsiSI CCGG 1 cut(s) 392
BslI CCNNNNNNNGG 2 cut(s) 398, 562
BsmAI GTCTC 2 cut(s) 81, 301
BsmI GAATGC 1 cut(s) 838
BsnI GGCC 4 cut(s) 70, 241, 387, 933
BsoBI CYCGRG 1 cut(s) 267
Bsp1407I TGTACA 1 cut(s) 346
Bsp143I GATC 6 cut(s) 340, 823, 916, 961, 1009, 1018
Bsp19I CCATGG 1 cut(s) 790
BspANI GGCC 4 cut(s) 70, 241, 387, 933
BspCNI CTCAG 1 cut(s) 308
BspHI TCATGA 1 cut(s) 364
BspLI GGNNCC 2 cut(s) 141, 402
BsrFI RCCGGY 1 cut(s) 391
BsrGI TGTACA 1 cut(s) 346
BsrI ACTGG 3 cut(s) 388, 455, 911
BssAI RCCGGY 1 cut(s) 391
BssECI CCNNGG 3 cut(s) 266, 502, 790
BssMI GATC 6 cut(s) 340, 823, 916, 961, 1009, 1018
BssNAI GTATAC 1 cut(s) 190
BssT1I CCWWGG 2 cut(s) 502, 790
Bst1107I GTATAC 1 cut(s) 190
Bst2UI CCWGG 1 cut(s) 246
Bst4CI ACNGT 5 cut(s) 289, 335, 535, 581, 907
Bst6I CTCTTC 2 cut(s) 22, 30
BstAPI GCANNNNNTGC 1 cut(s) 1052
BstAUI TGTACA 1 cut(s) 346
BstC8I GCNNGC 3 cut(s) 389, 711, 1122
BstDEI CTNAG 1 cut(s) 295
BstDSI CCRYGG 1 cut(s) 790
BstF5I GGATG 2 cut(s) 234, 1130
BstKTI GATC 6 cut(s) 343, 826, 919, 964, 1012, 1021
BstMAI GTCTC 2 cut(s) 81, 301
BstMBI GATC 6 cut(s) 340, 823, 916, 961, 1009, 1018
BstMWI GCNNNNNNNGC 4 cut(s) 719, 1052, 1088, 1168
BstNI CCWGG 1 cut(s) 246
BstNSI RCATGY 2 cut(s) 314, 419
BstSCI CCNGG 1 cut(s) 244
BstSFI CTRYAG 2 cut(s) 99, 1038
BstV1I GCAGC 2 cut(s) 968, 1091
BstV2I GAAGAC 1 cut(s) 365
BstZ17I GTATAC 1 cut(s) 190
BsuI GTATCC 2 cut(s) 563, 753
BsuRI GGCC 4 cut(s) 70, 241, 387, 933
BtgI CCRYGG 1 cut(s) 790
BtsCI GGATG 2 cut(s) 234, 1130
Cac8I GCNNGC 3 cut(s) 389, 711, 1122
CciI TCATGA 1 cut(s) 364
Cfr10I RCCGGY 1 cut(s) 391
Cfr13I GGNCC 5 cut(s) 263, 401, 597, 629, 931
Csp6I GTAC 4 cut(s) 174, 347, 458, 908
CviQI GTAC 4 cut(s) 174, 347, 458, 908
DdeI CTNAG 1 cut(s) 295
DpnI GATC 6 cut(s) 342, 825, 918, 963, 1011, 1020
DpnII GATC 6 cut(s) 340, 823, 916, 961, 1009, 1018
Eam1104I CTCTTC 2 cut(s) 22, 30
EarI CTCTTC 2 cut(s) 22, 30
Eco130I CCWWGG 2 cut(s) 502, 790
Eco47I GGWCC 4 cut(s) 263, 401, 597, 629
Eco57I CTGAAG 1 cut(s) 996
Eco88I CYCGRG 1 cut(s) 267
EcoO109I RGGNCCY 1 cut(s) 401
EcoRII CCWGG 1 cut(s) 244
EcoT14I CCWWGG 2 cut(s) 502, 790
EcoT22I ATGCAT 2 cut(s) 421, 1150
ErhI CCWWGG 2 cut(s) 502, 790
FbaI TGATCA 2 cut(s) 340, 1009
FblI GTMKAC 1 cut(s) 189
Fnu4HI GCNGC 3 cut(s) 957, 1080, 1184
FokI GGATG 2 cut(s) 241, 1117
Fsp4HI GCNGC 3 cut(s) 957, 1080, 1184
FspBI CTAG 2 cut(s) 492, 549
GluI GCNGC 3 cut(s) 957, 1080, 1184
GsuI CTGGAG 1 cut(s) 228
HaeIII GGCC 4 cut(s) 70, 241, 387, 933
HapII CCGG 1 cut(s) 392
HincII GTYRAC 1 cut(s) 775
HindII GTYRAC 1 cut(s) 775
HinfI GANTC 8 cut(s) 14, 50, 104, 235, 293, 495, 507, 590
HpaII CCGG 1 cut(s) 392
HphI GGTGA 5 cut(s) 260, 286, 577, 663, 1143
Hpy166II GTNNAC 4 cut(s) 108, 190, 292, 775
Hpy188I TCNGA 3 cut(s) 298, 446, 478
Hpy188III TCNNGA 5 cut(s) 80, 216, 269, 365, 511
Hpy8I GTNNAC 4 cut(s) 108, 190, 292, 775
Hpy99I CGWCG 2 cut(s) 637, 640
HpyAV CCTTC 2 cut(s) 426, 1081
HpyCH4III ACNGT 5 cut(s) 289, 335, 535, 581, 907
HpyCH4IV ACGT 1 cut(s) 426
HpyF10VI GCNNNNNNNGC 4 cut(s) 719, 1052, 1088, 1168
HpyF3I CTNAG 1 cut(s) 295
HpySE526I ACGT 1 cut(s) 426
Ksp22I TGATCA 2 cut(s) 340, 1009
Kzo9I GATC 6 cut(s) 340, 823, 916, 961, 1009, 1018
LmnI GCTCC 2 cut(s) 100, 139
Lsp1109I GCAGC 2 cut(s) 968, 1091
LweI GCATC 3 cut(s) 1033, 1139, 1157
MaeI CTAG 2 cut(s) 492, 549
MaeII ACGT 1 cut(s) 426
MaeIII GTNAC 2 cut(s) 647, 901
MalI GATC 6 cut(s) 342, 825, 918, 963, 1011, 1020
MboI GATC 6 cut(s) 340, 823, 916, 961, 1009, 1018
MboII GAAGA 5 cut(s) 9, 17, 365, 465, 854
MfeI CAATTG 1 cut(s) 1050
MluCI AATT 7 cut(s) 132, 195, 320, 657, 853, 1013, 1050
MlyI GAGTC 4 cut(s) 8, 113, 287, 584
MmeI TCCRAC 4 cut(s) 284, 409, 446, 521
Mph1103I ATGCAT 2 cut(s) 421, 1150
MseI TTAA 1 cut(s) 878
MslI CAYNNNNRTG 6 cut(s) 348, 579, 617, 676, 831, 969
MspI CCGG 1 cut(s) 392
MspR9I CCNGG 1 cut(s) 246
MunI CAATTG 1 cut(s) 1050
Mva1269I GAATGC 1 cut(s) 838
MvaI CCWGG 1 cut(s) 246
MwoI GCNNNNNNNGC 4 cut(s) 719, 1052, 1088, 1168
NcoI CCATGG 1 cut(s) 790
NdeII GATC 6 cut(s) 340, 823, 916, 961, 1009, 1018
NlaIV GGNNCC 2 cut(s) 141, 402
NsiI ATGCAT 2 cut(s) 421, 1150
NspI RCATGY 2 cut(s) 314, 419
PagI TCATGA 1 cut(s) 364
PciI ACATGT 1 cut(s) 310
PctI GAATGC 1 cut(s) 838
PfeI GAWTC 4 cut(s) 50, 235, 495, 507
PkrI GCNGC 3 cut(s) 958, 1081, 1185
PleI GAGTC 4 cut(s) 8, 112, 287, 584
PpsI GAGTC 4 cut(s) 8, 112, 287, 584
PpuMI RGGWCCY 1 cut(s) 401
PscI ACATGT 1 cut(s) 310
Psp5II RGGWCCY 1 cut(s) 401
Psp6I CCWGG 1 cut(s) 244
PspGI CCWGG 1 cut(s) 244
PspN4I GGNNCC 2 cut(s) 141, 402
PspPI GGNCC 5 cut(s) 263, 401, 597, 629, 931
PspPPI RGGWCCY 1 cut(s) 401
RsaI GTAC 4 cut(s) 175, 348, 459, 909
RsaNI GTAC 4 cut(s) 174, 347, 458, 908
RseI CAYNNNNRTG 6 cut(s) 348, 579, 617, 676, 831, 969
SaqAI TTAA 1 cut(s) 878
SatI GCNGC 3 cut(s) 957, 1080, 1184
Sau3AI GATC 6 cut(s) 340, 823, 916, 961, 1009, 1018
Sau96I GGNCC 5 cut(s) 263, 401, 597, 629, 931
SchI GAGTC 4 cut(s) 8, 113, 287, 584
ScrFI CCNGG 1 cut(s) 246
SfaNI GCATC 3 cut(s) 1033, 1139, 1157
SfcI CTRYAG 2 cut(s) 99, 1038
SinI GGWCC 4 cut(s) 263, 401, 597, 629
SmiMI CAYNNNNRTG 6 cut(s) 348, 579, 617, 676, 831, 969
SmlI CTYRAG 1 cut(s) 80
SmoI CTYRAG 1 cut(s) 80
Sse9I AATT 7 cut(s) 132, 195, 320, 657, 853, 1013, 1050
SspMI CTAG 2 cut(s) 492, 549
StyD4I CCNGG 1 cut(s) 244
StyI CCWWGG 2 cut(s) 502, 790
TaaI ACNGT 5 cut(s) 289, 335, 535, 581, 907
TaiI ACGT 1 cut(s) 429
TaqI TCGA 3 cut(s) 406, 585, 915
TasI AATT 7 cut(s) 132, 195, 320, 657, 853, 1013, 1050
TatI WGTACW 1 cut(s) 346
TfiI GAWTC 4 cut(s) 50, 235, 495, 507
Tru1I TTAA 1 cut(s) 878
Tru9I TTAA 1 cut(s) 878
TseI GCWGC 3 cut(s) 956, 1079, 1183
TspDTI ATGAA 6 cut(s) 9, 344, 353, 365, 584, 1119
TspGWI ACGGA 1 cut(s) 621
VpaK11BI GGWCC 4 cut(s) 263, 401, 597, 629
XapI RAATTY 4 cut(s) 132, 320, 657, 853
XceI RCATGY 2 cut(s) 314, 419
XcmI CCANNNNNNNNNTGG 1 cut(s) 462
XmiI GTMKAC 1 cut(s) 189
XspI CTAG 2 cut(s) 492, 549
Zsp2I ATGCAT 2 cut(s) 421, 1150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.