RchiOBHm_Chr7g0198501

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0005942
Physical Location & Seq
Reverse (-)
16389824 .. 16392033
2210 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17760

Sequence Viewer

Length: 915 bp
ATGGCCGAAACAACCATCGATTCTCAGTCAAAGAGAATTTGTGTTGTCACCGGGGCCAACAAAGGGATTGGATTTGAGATATGCAGGCAACTAGCTTCTAATGGGATCCTGGTAGTATTAACTGCAAGAGATGTGAAGAGCGGACAAGAAGCTGTCGAAAAGCTCAAGCTTTCTGGCTTGACTGATGTAATTTTTCATCAACTAGATGTAACCGATGCAACTAGCATTGCTTCTTTAGCAGATTTCATCGAATCCCAGTTCGGGAAGCTTGACATATTGGTCAACAATGCAGGAGTTAACGGAATCACAGTGATCGGAGGGAATGCAAAGAACATAACTTTTGATTATGAGCATATGTTTGGTTCAAAAGCTGGAGACTTGAAAAAGCATCTAGAGCAAACATACGAAAGGGCAGAGAAATGTCTGAAAACGAACTACTATGGAATCAAGCAAATGAGCAAAGCAACTATTCGACTTCTGCAAAAATCTGGTTCACCTAAAATGGTCAATGTCTCTTCCATCGGAGGACAGTTAAAGCGTGTTACGCATCAGAAAGCAAGGGATGAACTAGGAGATGTTCAAGGCCTCACCGAAGAGAAAGTGGACAAGGTTGTTGAGGAGTTTCTGAAGGATGCGAAAGAGAATTTGGTTGAAGCTAATGGCTGGCCTACAAACTTCTCTGCTTACGTAGTGTCCAAGGCAGCCATGAATGCTTACACCAGAGTTCTGGCAAAGAGCCACCCCGAAATTGCAATCAATGCAGTTAGTCCTGGCTACACTAGCACTGACATGACCTTCAACGTTGGCAGGCACTTTGTTGAAGAAGGTGCAAAGGGTCCTGTTAAGCTGGCCTTGAGTCCTAAAGGTGGACCTTCTAGCCTCTATTTTGACCAGCTTGAAGTCTCATCGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

304

Amino Acids

33.0

Weight (kDa)

7.68

Isoelectric Point (pI)

30.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 13 - 179 1.7e-26 short chain dehydrogenase
KR PF08659 15 - 101 9.8e-07 KR domain
adh_short_C2 PF13561 18 - 177 3.1e-18 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 224 - 269 1.4e-06 Enoyl-(Acyl carrier protein) reductase
adh_short PF00106 225 - 267 1.3e-06 short chain dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 278
AccBSI CCGCTC 1 cut(s) 141
AciI CCGC 1 cut(s) 141
AclI AACGTT 1 cut(s) 801
AclWI GGATC 2 cut(s) 100, 113
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 36, 643
AcuI CTGAAG 1 cut(s) 647
AfiI CCNNNNNNNGG 3 cut(s) 63, 261, 866
AgsI TTSAA 7 cut(s) 366, 382, 581, 653, 799, 821, 899
AjnI CCWGG 2 cut(s) 108, 769
AjuI GAANNNNNNNTTGG 2 cut(s) 629, 661
AluBI AGCT 9 cut(s) 95, 152, 163, 169, 268, 371, 656, 847, 895
AluI AGCT 9 cut(s) 95, 152, 163, 169, 268, 371, 656, 847, 895
Alw26I GTCTC 3 cut(s) 369, 517, 907
AlwI GGATC 2 cut(s) 100, 113
AoxI GGCC 5 cut(s) 3, 54, 583, 665, 849
ApeKI GCWGC 1 cut(s) 701
ApoI RAATTY 2 cut(s) 36, 643
AspS9I GGNCC 3 cut(s) 54, 836, 869
AsuC2I CCSGG 1 cut(s) 52
AsuHPI GGTGA 3 cut(s) 40, 486, 580
AvaII GGWCC 2 cut(s) 836, 869
BamHI GGATCC 1 cut(s) 105
BbvI GCAGC 1 cut(s) 713
BccI CCATC 2 cut(s) 23, 527
BciT130I CCWGG 2 cut(s) 110, 771
BcnI CCSGG 1 cut(s) 52
BcoDI GTCTC 3 cut(s) 369, 517, 907
BfaI CTAG 7 cut(s) 92, 203, 222, 392, 569, 780, 876
BisI GCNGC 1 cut(s) 702
BlsI GCNGC 1 cut(s) 703
Bme1390I CCNGG 3 cut(s) 52, 110, 771
Bme18I GGWCC 2 cut(s) 836, 869
BmgT120I GGNCC 3 cut(s) 54, 836, 869
BmiI GGNNCC 3 cut(s) 55, 107, 837
BmrFI CCNGG 3 cut(s) 52, 110, 771
BmrI ACTGGG 1 cut(s) 250
BmsI GCATC 4 cut(s) 205, 397, 556, 622
BmuI ACTGGG 1 cut(s) 250
BpmI CTGGAG 1 cut(s) 393
BpuEI CTTGAG 2 cut(s) 149, 874
BpuMI CCSGG 1 cut(s) 52
Bsa29I ATCGAT 1 cut(s) 18
BsaAI YACGTR 1 cut(s) 688
BsaJI CCNNGG 2 cut(s) 51, 696
Bsc4I CCNNNNNNNGG 3 cut(s) 63, 261, 866
Bse1I ACTGG 1 cut(s) 256
Bse3DI GCAATG 1 cut(s) 225
BseBI CCWGG 2 cut(s) 110, 771
BseCI ATCGAT 1 cut(s) 18
BseDI CCNNGG 2 cut(s) 51, 696
BseGI GGATG 2 cut(s) 568, 637
BseLI CCNNNNNNNGG 3 cut(s) 63, 261, 866
BseMI GCAATG 1 cut(s) 225
BseMII CTCAG 1 cut(s) 38
BseNI ACTGG 1 cut(s) 256
BseRI GAGGAG 1 cut(s) 632
BseXI GCAGC 1 cut(s) 713
BshFI GGCC 5 cut(s) 5, 56, 585, 667, 851
BshVI ATCGAT 1 cut(s) 18
BsiSI CCGG 1 cut(s) 51
BslI CCNNNNNNNGG 3 cut(s) 63, 261, 866
BsmAI GTCTC 3 cut(s) 369, 517, 907
BsmI GAATGC 2 cut(s) 328, 715
BsnI GGCC 5 cut(s) 5, 56, 585, 667, 851
Bsp143I GATC 2 cut(s) 105, 312
BspACI CCGC 1 cut(s) 141
BspANI GGCC 5 cut(s) 5, 56, 585, 667, 851
BspCNI CTCAG 1 cut(s) 37
BspDI ATCGAT 1 cut(s) 18
BspLI GGNNCC 3 cut(s) 55, 107, 837
BspPI GGATC 2 cut(s) 100, 113
BspQI GCTCTTC 1 cut(s) 131
BsrBI CCGCTC 1 cut(s) 141
BsrDI GCAATG 1 cut(s) 225
BsrI ACTGG 1 cut(s) 256
BssECI CCNNGG 2 cut(s) 51, 696
BssMI GATC 2 cut(s) 105, 312
BssT1I CCWWGG 1 cut(s) 696
Bst2UI CCWGG 2 cut(s) 110, 771
Bst4CI ACNGT 2 cut(s) 310, 531
Bst6I CTCTTC 3 cut(s) 131, 520, 588
BstAPI GCANNNNNTGC 1 cut(s) 758
BstBAI YACGTR 1 cut(s) 688
BstC8I GCNNGC 4 cut(s) 86, 665, 809, 849
BstDEI CTNAG 1 cut(s) 24
BstF5I GGATG 2 cut(s) 568, 637
BstKTI GATC 2 cut(s) 108, 315
BstMAI GTCTC 3 cut(s) 369, 517, 907
BstMBI GATC 2 cut(s) 105, 312
BstMWI GCNNNNNNNGC 6 cut(s) 236, 394, 544, 710, 758, 780
BstNI CCWGG 2 cut(s) 110, 771
BstSCI CCNGG 3 cut(s) 50, 108, 769
BstSNI TACGTA 1 cut(s) 688
BstV1I GCAGC 1 cut(s) 713
BstX2I RGATCY 1 cut(s) 105
BstXI CCANNNNNNTGG 1 cut(s) 727
BstYI RGATCY 1 cut(s) 105
Bsu15I ATCGAT 1 cut(s) 18
BsuRI GGCC 5 cut(s) 5, 56, 585, 667, 851
BsuTUI ATCGAT 1 cut(s) 18
BtsCI GGATG 2 cut(s) 568, 637
BtsIMutI CAGTG 2 cut(s) 315, 783
Cac8I GCNNGC 4 cut(s) 86, 665, 809, 849
Cfr13I GGNCC 3 cut(s) 54, 836, 869
ClaI ATCGAT 1 cut(s) 18
CviAII CATG 2 cut(s) 706, 790
DdeI CTNAG 1 cut(s) 24
DpnI GATC 2 cut(s) 107, 314
DpnII GATC 2 cut(s) 105, 312
DrdI GACNNNNNNGTC 1 cut(s) 278
DseDI GACNNNNNNGTC 1 cut(s) 278
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 131, 520, 588
EarI CTCTTC 3 cut(s) 131, 520, 588
Eco105I TACGTA 1 cut(s) 688
Eco130I CCWWGG 1 cut(s) 696
Eco147I AGGCCT 1 cut(s) 585
Eco47I GGWCC 2 cut(s) 836, 869
Eco57I CTGAAG 1 cut(s) 647
EcoO109I RGGNCCY 1 cut(s) 836
EcoRII CCWGG 2 cut(s) 108, 769
EcoT14I CCWWGG 1 cut(s) 696
ErhI CCWWGG 1 cut(s) 696
FaeI CATG 2 cut(s) 709, 793
FalI AAGNNNNNCTT 2 cut(s) 836, 868
FatI CATG 2 cut(s) 705, 789
FauNDI CATATG 1 cut(s) 354
Fnu4HI GCNGC 1 cut(s) 702
FokI GGATG 2 cut(s) 575, 644
Fsp4HI GCNGC 1 cut(s) 702
FspBI CTAG 7 cut(s) 92, 203, 222, 392, 569, 780, 876
GluI GCNGC 1 cut(s) 702
GsuI CTGGAG 1 cut(s) 393
HaeIII GGCC 5 cut(s) 5, 56, 585, 667, 851
HapII CCGG 1 cut(s) 51
Hin1II CATG 2 cut(s) 709, 793
HincII GTYRAC 2 cut(s) 283, 298
HindII GTYRAC 2 cut(s) 283, 298
HindIII AAGCTT 2 cut(s) 167, 266
HinfI GANTC 5 cut(s) 20, 251, 303, 444, 856
HpaI GTTAAC 1 cut(s) 298
HpaII CCGG 1 cut(s) 51
HphI GGTGA 3 cut(s) 40, 486, 580
Hpy166II GTNNAC 5 cut(s) 283, 298, 494, 604, 869
Hpy188I TCNGA 5 cut(s) 317, 426, 524, 552, 627
Hpy188III TCNNGA 2 cut(s) 262, 392
Hpy8I GTNNAC 5 cut(s) 283, 298, 494, 604, 869
HpyAV CCTTC 4 cut(s) 622, 805, 818, 882
HpyCH4III ACNGT 2 cut(s) 310, 531
HpyCH4IV ACGT 2 cut(s) 687, 801
HpyCH4V TGCA 9 cut(s) 84, 125, 218, 290, 326, 481, 752, 761, 830
HpyF10VI GCNNNNNNNGC 6 cut(s) 236, 394, 544, 710, 758, 780
HpyF3I CTNAG 1 cut(s) 24
HpySE526I ACGT 2 cut(s) 687, 801
Hsp92II CATG 2 cut(s) 709, 793
KspAI GTTAAC 1 cut(s) 298
Kzo9I GATC 2 cut(s) 105, 312
LguI GCTCTTC 1 cut(s) 131
Lsp1109I GCAGC 1 cut(s) 713
LweI GCATC 4 cut(s) 205, 397, 556, 622
MaeI CTAG 7 cut(s) 92, 203, 222, 392, 569, 780, 876
MaeII ACGT 2 cut(s) 687, 801
MaeIII GTNAC 3 cut(s) 46, 208, 541
MalI GATC 2 cut(s) 107, 314
MbiI CCGCTC 1 cut(s) 141
MboI GATC 2 cut(s) 105, 312
MboII GAAGA 4 cut(s) 148, 507, 605, 833
MflI RGATCY 1 cut(s) 105
MluCI AATT 4 cut(s) 36, 189, 643, 747
MlyI GAGTC 1 cut(s) 865
MnlI CCTC 5 cut(s) 311, 518, 596, 610, 890
MseI TTAA 4 cut(s) 119, 297, 533, 843
MslI CAYNNNNRTG 1 cut(s) 788
MspI CCGG 1 cut(s) 51
MspR9I CCNGG 3 cut(s) 52, 110, 771
Mva1269I GAATGC 2 cut(s) 328, 715
MvaI CCWGG 2 cut(s) 110, 771
MwoI GCNNNNNNNGC 6 cut(s) 236, 394, 544, 710, 758, 780
NciI CCSGG 1 cut(s) 52
NdeI CATATG 1 cut(s) 354
NdeII GATC 2 cut(s) 105, 312
NlaIII CATG 2 cut(s) 709, 793
NlaIV GGNNCC 3 cut(s) 55, 107, 837
NmuCI GTSAC 1 cut(s) 46
PceI AGGCCT 1 cut(s) 585
PciSI GCTCTTC 1 cut(s) 131
PctI GAATGC 2 cut(s) 328, 715
PfeI GAWTC 4 cut(s) 20, 251, 303, 444
PkrI GCNGC 1 cut(s) 703
PleI GAGTC 1 cut(s) 864
PpsI GAGTC 1 cut(s) 864
Ppu21I YACGTR 1 cut(s) 688
PpuMI RGGWCCY 1 cut(s) 836
Psp1406I AACGTT 1 cut(s) 801
Psp5II RGGWCCY 1 cut(s) 836
Psp6I CCWGG 2 cut(s) 108, 769
PspGI CCWGG 2 cut(s) 108, 769
PspN4I GGNNCC 3 cut(s) 55, 107, 837
PspPI GGNCC 3 cut(s) 54, 836, 869
PspPPI RGGWCCY 1 cut(s) 836
PsuI RGATCY 1 cut(s) 105
RseI CAYNNNNRTG 1 cut(s) 788
SapI GCTCTTC 1 cut(s) 131
SaqAI TTAA 4 cut(s) 119, 297, 533, 843
SatI GCNGC 1 cut(s) 702
Sau3AI GATC 2 cut(s) 105, 312
Sau96I GGNCC 3 cut(s) 54, 836, 869
SchI GAGTC 1 cut(s) 865
ScrFI CCNGG 3 cut(s) 52, 110, 771
SfaNI GCATC 4 cut(s) 205, 397, 556, 622
SinI GGWCC 2 cut(s) 836, 869
SmiMI CAYNNNNRTG 1 cut(s) 788
SmlI CTYRAG 2 cut(s) 164, 853
SmoI CTYRAG 2 cut(s) 164, 853
SnaBI TACGTA 1 cut(s) 688
Sse9I AATT 4 cut(s) 36, 189, 643, 747
SseBI AGGCCT 1 cut(s) 585
SsiI CCGC 1 cut(s) 141
SspMI CTAG 7 cut(s) 92, 203, 222, 392, 569, 780, 876
StuI AGGCCT 1 cut(s) 585
StyD4I CCNGG 3 cut(s) 50, 108, 769
StyI CCWWGG 1 cut(s) 696
TaaI ACNGT 2 cut(s) 310, 531
TaiI ACGT 2 cut(s) 690, 804
TaqI TCGA 4 cut(s) 18, 156, 249, 472
TasI AATT 4 cut(s) 36, 189, 643, 747
TfiI GAWTC 4 cut(s) 20, 251, 303, 444
Tru1I TTAA 4 cut(s) 119, 297, 533, 843
Tru9I TTAA 4 cut(s) 119, 297, 533, 843
TscAI CASTG 2 cut(s) 315, 790
TseFI GTSAC 1 cut(s) 46
TseI GCWGC 1 cut(s) 701
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 4 cut(s) 185, 235, 579, 722
TspGWI ACGGA 1 cut(s) 315
TspRI CASTG 2 cut(s) 315, 790
VpaK11BI GGWCC 2 cut(s) 836, 869
XapI RAATTY 2 cut(s) 36, 643
XbaI TCTAGA 1 cut(s) 391
XspI CTAG 7 cut(s) 92, 203, 222, 392, 569, 780, 876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.