RLG00000003863

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0005942
Physical Location & Seq
Forward (+)
53673330 .. 53675881
2552 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003863

Sequence Viewer

Length: 918 bp
ATGGCCGAAACAACCATCGATTCTCAGTCAAAGAGAATTTGTGTTGTCACCGGGGCCAACAAAGGGATTGGATTTGAGATATGCAGGCAACTAGCTTCTAATGGGATCCTGGTAATATTAACTGCAAGGGATGTGAAGAGCGGACAAGAAGCTGTCGAAAAGCTCAAGCTTTCTGGCTTGACTGATGTAATTTTTCATCAACTAGATGTAACCGATGCAACTAGCATTGCCTCTTTAGTAGATTTCATCGAATCCCAGTTCGGGAAGCTTGACATATTGGTCAACAATGCAGCAATTAACGGAGTCACAATGATCGGAGGGAATGCAAAGAACGTAACTTTTGATTATGAACATATGTTTGGTTCAAAAGCTGGAGACCTGAAAAAGCATCTAGAGCAAACATACGAAAGTGCAGAGAAATGTCTGAAAACGAACTACTATGGAATCAAGCAAATGAGTAAAGCAACTATTCGACTTCTGCAAAAATCTGGTTCACCGAAAATGGTCAATGTCTCTTCCAACGGAGGACAGTTAAAGTATGTTATGCATCAGAAAGCAAGGGATGAGCTAGGAGATGTTCAAGGCCTCACCGAAGAGAAAGTGGACAAGGTTGTTGAGGAGTTTCTGAAGGATGCGAAAGAGAATTTGGTCGAAGCTAATGGCTGGCCTATAAACTTCTCTGCTTACGTAGTGTCCAAGGCAGCCGTGAATGCTTACACCAGAGTTCTGGCAAAGAGCTTCCCCGAAATTGCGATCAATGCAGTTGGTCCTGGCTACACTAGCACTGACATGACCTTCAACGTTGGCGGGCACTCTGTTGAAGAAGGTGCAAAGGGTCCTGTTAAGCTGGCCTTGGGCCTTGATGGTGGACCTTCTGGCCTCTATTTTGACCAACTTGAAGTCTCATCTTTTGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

306

Amino Acids

32.92

Weight (kDa)

5.67

Isoelectric Point (pI)

28.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 13 - 181 3.1e-26 short chain dehydrogenase
KR PF08659 15 - 101 1.2e-06 KR domain
adh_short_C2 PF13561 18 - 178 5.1e-18 Enoyl-(Acyl carrier protein) reductase
adh_short PF00106 224 - 266 1.6e-06 short chain dehydrogenase
adh_short_C2 PF13561 225 - 270 4.4e-06 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 278
AccBSI CCGCTC 1 cut(s) 141
AciI CCGC 2 cut(s) 141, 807
AclI AACGTT 1 cut(s) 801
AclWI GGATC 2 cut(s) 100, 113
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 36, 643
AcuI CTGAAG 1 cut(s) 647
AfiI CCNNNNNNNGG 2 cut(s) 63, 261
AgsI TTSAA 6 cut(s) 366, 581, 799, 821, 899, 914
AjnI CCWGG 2 cut(s) 108, 769
AjuI GAANNNNNNNTTGG 4 cut(s) 342, 374, 629, 661
Alw26I GTCTC 3 cut(s) 369, 517, 907
AlwI GGATC 2 cut(s) 100, 113
AoxI GGCC 7 cut(s) 3, 54, 583, 665, 849, 856, 877
ApeKI GCWGC 2 cut(s) 290, 701
ApoI RAATTY 2 cut(s) 36, 643
AspS9I GGNCC 5 cut(s) 54, 767, 836, 856, 869
AsuC2I CCSGG 1 cut(s) 52
AsuHPI GGTGA 3 cut(s) 40, 486, 580
AvaII GGWCC 3 cut(s) 767, 836, 869
BaeGI GKGCMC 1 cut(s) 813
BamHI GGATCC 1 cut(s) 105
BbvI GCAGC 2 cut(s) 302, 713
BccI CCATC 2 cut(s) 23, 857
BceAI ACGGC 1 cut(s) 689
BciT130I CCWGG 2 cut(s) 110, 771
BcnI CCSGG 1 cut(s) 52
BcoDI GTCTC 3 cut(s) 369, 517, 907
BfaI CTAG 6 cut(s) 92, 203, 222, 392, 569, 780
BisI GCNGC 2 cut(s) 291, 702
BlsI GCNGC 2 cut(s) 292, 703
Bme1390I CCNGG 3 cut(s) 52, 110, 771
Bme18I GGWCC 3 cut(s) 767, 836, 869
BmgT120I GGNCC 5 cut(s) 54, 767, 836, 856, 869
BmiI GGNNCC 3 cut(s) 55, 107, 837
BmrFI CCNGG 3 cut(s) 52, 110, 771
BmrI ACTGGG 1 cut(s) 250
BmsI GCATC 4 cut(s) 205, 397, 556, 622
BmuI ACTGGG 1 cut(s) 250
BpmI CTGGAG 1 cut(s) 393
BpuEI CTTGAG 1 cut(s) 149
BpuMI CCSGG 1 cut(s) 52
Bsa29I ATCGAT 1 cut(s) 18
BsaAI YACGTR 1 cut(s) 688
BsaI GGTCTC 1 cut(s) 369
BsaJI CCNNGG 3 cut(s) 51, 696, 852
Bsc4I CCNNNNNNNGG 2 cut(s) 63, 261
Bse1I ACTGG 1 cut(s) 256
Bse3DI GCAATG 1 cut(s) 225
BseBI CCWGG 2 cut(s) 110, 771
BseCI ATCGAT 1 cut(s) 18
BseDI CCNNGG 3 cut(s) 51, 696, 852
BseGI GGATG 3 cut(s) 136, 568, 637
BseLI CCNNNNNNNGG 2 cut(s) 63, 261
BseMI GCAATG 1 cut(s) 225
BseMII CTCAG 1 cut(s) 38
BseNI ACTGG 1 cut(s) 256
BseRI GAGGAG 1 cut(s) 632
BseSI GKGCMC 1 cut(s) 813
BseXI GCAGC 2 cut(s) 302, 713
BsgI GTGCAG 1 cut(s) 432
BshFI GGCC 7 cut(s) 5, 56, 585, 667, 851, 858, 879
BshVI ATCGAT 1 cut(s) 18
BsiSI CCGG 1 cut(s) 51
BslI CCNNNNNNNGG 2 cut(s) 63, 261
BsmAI GTCTC 3 cut(s) 369, 517, 907
BsmI GAATGC 2 cut(s) 328, 715
BsnI GGCC 7 cut(s) 5, 56, 585, 667, 851, 858, 879
Bso31I GGTCTC 1 cut(s) 369
Bsp1286I GDGCHC 1 cut(s) 813
Bsp143I GATC 3 cut(s) 105, 312, 753
BspACI CCGC 2 cut(s) 141, 807
BspANI GGCC 7 cut(s) 5, 56, 585, 667, 851, 858, 879
BspCNI CTCAG 1 cut(s) 37
BspDI ATCGAT 1 cut(s) 18
BspLI GGNNCC 3 cut(s) 55, 107, 837
BspPI GGATC 2 cut(s) 100, 113
BspQI GCTCTTC 1 cut(s) 131
BspTNI GGTCTC 1 cut(s) 369
BsrBI CCGCTC 1 cut(s) 141
BsrDI GCAATG 1 cut(s) 225
BsrI ACTGG 1 cut(s) 256
BssECI CCNNGG 3 cut(s) 51, 696, 852
BssMI GATC 3 cut(s) 105, 312, 753
BssT1I CCWWGG 2 cut(s) 696, 852
Bst2UI CCWGG 2 cut(s) 110, 771
Bst4CI ACNGT 1 cut(s) 531
Bst6I CTCTTC 3 cut(s) 131, 520, 588
BstBAI YACGTR 1 cut(s) 688
BstC8I GCNNGC 4 cut(s) 86, 665, 809, 849
BstDEI CTNAG 1 cut(s) 24
BstF5I GGATG 3 cut(s) 136, 568, 637
BstKTI GATC 3 cut(s) 108, 315, 756
BstMAI GTCTC 3 cut(s) 369, 517, 907
BstMBI GATC 3 cut(s) 105, 312, 753
BstMWI GCNNNNNNNGC 4 cut(s) 394, 710, 758, 780
BstNI CCWGG 2 cut(s) 110, 771
BstSCI CCNGG 3 cut(s) 50, 108, 769
BstSLI GKGCMC 1 cut(s) 813
BstSNI TACGTA 1 cut(s) 688
BstV1I GCAGC 2 cut(s) 302, 713
BstX2I RGATCY 1 cut(s) 105
BstXI CCANNNNNNTGG 1 cut(s) 727
BstYI RGATCY 1 cut(s) 105
Bsu15I ATCGAT 1 cut(s) 18
BsuRI GGCC 7 cut(s) 5, 56, 585, 667, 851, 858, 879
BsuTUI ATCGAT 1 cut(s) 18
BtsCI GGATG 3 cut(s) 136, 568, 637
BtsIMutI CAGTG 1 cut(s) 783
Cac8I GCNNGC 4 cut(s) 86, 665, 809, 849
Cfr13I GGNCC 5 cut(s) 54, 767, 836, 856, 869
ClaI ATCGAT 1 cut(s) 18
CviAII CATG 1 cut(s) 790
DdeI CTNAG 1 cut(s) 24
DpnI GATC 3 cut(s) 107, 314, 755
DpnII GATC 3 cut(s) 105, 312, 753
DrdI GACNNNNNNGTC 1 cut(s) 278
DseDI GACNNNNNNGTC 1 cut(s) 278
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 131, 520, 588
EarI CTCTTC 3 cut(s) 131, 520, 588
Eco105I TACGTA 1 cut(s) 688
Eco130I CCWWGG 2 cut(s) 696, 852
Eco147I AGGCCT 1 cut(s) 585
Eco31I GGTCTC 1 cut(s) 369
Eco47I GGWCC 3 cut(s) 767, 836, 869
Eco57I CTGAAG 1 cut(s) 647
EcoO109I RGGNCCY 1 cut(s) 836
EcoRII CCWGG 2 cut(s) 108, 769
EcoT14I CCWWGG 2 cut(s) 696, 852
EcoT22I ATGCAT 1 cut(s) 549
ErhI CCWWGG 2 cut(s) 696, 852
FaeI CATG 1 cut(s) 793
FalI AAGNNNNNCTT 2 cut(s) 836, 868
FatI CATG 1 cut(s) 789
FauI CCCGC 1 cut(s) 800
FauNDI CATATG 1 cut(s) 354
Fnu4HI GCNGC 2 cut(s) 291, 702
FokI GGATG 3 cut(s) 143, 575, 644
Fsp4HI GCNGC 2 cut(s) 291, 702
FspBI CTAG 6 cut(s) 92, 203, 222, 392, 569, 780
GluI GCNGC 2 cut(s) 291, 702
GsuI CTGGAG 1 cut(s) 393
HaeIII GGCC 7 cut(s) 5, 56, 585, 667, 851, 858, 879
HapII CCGG 1 cut(s) 51
Hin1II CATG 1 cut(s) 793
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HindIII AAGCTT 2 cut(s) 167, 266
HinfI GANTC 4 cut(s) 20, 251, 303, 444
HpaII CCGG 1 cut(s) 51
HphI GGTGA 3 cut(s) 40, 486, 580
Hpy166II GTNNAC 4 cut(s) 283, 494, 604, 869
Hpy188I TCNGA 4 cut(s) 317, 426, 552, 627
Hpy188III TCNNGA 2 cut(s) 262, 392
Hpy8I GTNNAC 4 cut(s) 283, 494, 604, 869
HpyAV CCTTC 4 cut(s) 622, 805, 818, 882
HpyCH4III ACNGT 1 cut(s) 531
HpyCH4IV ACGT 3 cut(s) 333, 687, 801
HpyF10VI GCNNNNNNNGC 4 cut(s) 394, 710, 758, 780
HpyF3I CTNAG 1 cut(s) 24
HpySE526I ACGT 3 cut(s) 333, 687, 801
Hsp92II CATG 1 cut(s) 793
Kzo9I GATC 3 cut(s) 105, 312, 753
LguI GCTCTTC 1 cut(s) 131
Lsp1109I GCAGC 2 cut(s) 302, 713
LweI GCATC 4 cut(s) 205, 397, 556, 622
MaeI CTAG 6 cut(s) 92, 203, 222, 392, 569, 780
MaeII ACGT 3 cut(s) 333, 687, 801
MaeIII GTNAC 4 cut(s) 46, 208, 304, 334
MalI GATC 3 cut(s) 107, 314, 755
MbiI CCGCTC 1 cut(s) 141
MboI GATC 3 cut(s) 105, 312, 753
MboII GAAGA 4 cut(s) 148, 507, 605, 833
MflI RGATCY 1 cut(s) 105
MhlI GDGCHC 1 cut(s) 813
MluCI AATT 5 cut(s) 36, 189, 294, 643, 747
MlyI GAGTC 1 cut(s) 312
MmeI TCCRAC 1 cut(s) 543
MnlI CCTC 6 cut(s) 241, 311, 518, 596, 610, 890
Mph1103I ATGCAT 1 cut(s) 549
MseI TTAA 4 cut(s) 119, 297, 533, 843
MslI CAYNNNNRTG 1 cut(s) 788
MspI CCGG 1 cut(s) 51
MspR9I CCNGG 3 cut(s) 52, 110, 771
Mva1269I GAATGC 2 cut(s) 328, 715
MvaI CCWGG 2 cut(s) 110, 771
MwoI GCNNNNNNNGC 4 cut(s) 394, 710, 758, 780
NciI CCSGG 1 cut(s) 52
NdeI CATATG 1 cut(s) 354
NdeII GATC 3 cut(s) 105, 312, 753
NlaIII CATG 1 cut(s) 793
NlaIV GGNNCC 3 cut(s) 55, 107, 837
NmuCI GTSAC 2 cut(s) 46, 304
NsiI ATGCAT 1 cut(s) 549
PceI AGGCCT 1 cut(s) 585
PciSI GCTCTTC 1 cut(s) 131
PctI GAATGC 2 cut(s) 328, 715
PfeI GAWTC 3 cut(s) 20, 251, 444
PkrI GCNGC 2 cut(s) 292, 703
PleI GAGTC 1 cut(s) 311
PpsI GAGTC 1 cut(s) 311
Ppu21I YACGTR 1 cut(s) 688
PpuMI RGGWCCY 1 cut(s) 836
Psp1406I AACGTT 1 cut(s) 801
Psp5II RGGWCCY 1 cut(s) 836
Psp6I CCWGG 2 cut(s) 108, 769
PspGI CCWGG 2 cut(s) 108, 769
PspN4I GGNNCC 3 cut(s) 55, 107, 837
PspPI GGNCC 5 cut(s) 54, 767, 836, 856, 869
PspPPI RGGWCCY 1 cut(s) 836
PsuI RGATCY 1 cut(s) 105
RseI CAYNNNNRTG 1 cut(s) 788
SapI GCTCTTC 1 cut(s) 131
SaqAI TTAA 4 cut(s) 119, 297, 533, 843
SatI GCNGC 2 cut(s) 291, 702
Sau3AI GATC 3 cut(s) 105, 312, 753
Sau96I GGNCC 5 cut(s) 54, 767, 836, 856, 869
SchI GAGTC 1 cut(s) 312
ScrFI CCNGG 3 cut(s) 52, 110, 771
SduI GDGCHC 1 cut(s) 813
SfaNI GCATC 4 cut(s) 205, 397, 556, 622
SinI GGWCC 3 cut(s) 767, 836, 869
SmiMI CAYNNNNRTG 1 cut(s) 788
SmlI CTYRAG 1 cut(s) 164
SmoI CTYRAG 1 cut(s) 164
SnaBI TACGTA 1 cut(s) 688
Sse9I AATT 5 cut(s) 36, 189, 294, 643, 747
SseBI AGGCCT 1 cut(s) 585
SsiI CCGC 2 cut(s) 141, 807
SspI AATATT 1 cut(s) 117
SspMI CTAG 6 cut(s) 92, 203, 222, 392, 569, 780
StuI AGGCCT 1 cut(s) 585
StyD4I CCNGG 3 cut(s) 50, 108, 769
StyI CCWWGG 2 cut(s) 696, 852
TaaI ACNGT 1 cut(s) 531
TaiI ACGT 3 cut(s) 336, 690, 804
TaqI TCGA 5 cut(s) 18, 156, 249, 472, 651
TasI AATT 5 cut(s) 36, 189, 294, 643, 747
TfiI GAWTC 3 cut(s) 20, 251, 444
Tru1I TTAA 4 cut(s) 119, 297, 533, 843
Tru9I TTAA 4 cut(s) 119, 297, 533, 843
TscAI CASTG 1 cut(s) 790
TseFI GTSAC 2 cut(s) 46, 304
TseI GCWGC 2 cut(s) 290, 701
Tsp45I GTSAC 2 cut(s) 46, 304
TspDTI ATGAA 3 cut(s) 185, 235, 363
TspGWI ACGGA 2 cut(s) 315, 537
TspRI CASTG 1 cut(s) 790
VpaK11BI GGWCC 3 cut(s) 767, 836, 869
XapI RAATTY 2 cut(s) 36, 643
XbaI TCTAGA 1 cut(s) 391
XspI CTAG 6 cut(s) 92, 203, 222, 392, 569, 780
Zsp2I ATGCAT 1 cut(s) 549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.