Rorug07G0058500

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0005942
Physical Location & Seq
Reverse (-)
4213913 .. 4214455
543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0058500.1

Sequence Viewer

Length: 543 bp
ATGCGTGAAAACCCTAATCCAGATGCAGATCCGAGCGAGCAGTTTTACGTGGGAGATAATGTATTGAGAAACACTGGTCAAGCTCTGGAGTTCAGGCAGATGAACATTCATGCTTTGGAAGCATTTGAGAGGGGACAAGATGTTCACATGCAAGCAGCTCCATCCCAAGCTGAGTTTCTTTATAAAAATTATAAGGCGATTAAGGAGAGATTGACTTCACAGACGAATTGCACCATTCTAGAAAAGTATGGCAATGCAGCTAGTGGAGAAGAACTTCCTAAGGAGCTTCTACTGGGACAGAGCGAAAGAGATGTTGAATACGATCGAGCTGGGAGGATCATCAAGGGCGAGGAAGAAGCGGTTCCGAGGAGCAAATATGCAGAAGATGTCAGTATCAACAACCACACTTGTGTGTGGGGTTCGTGGTGGAAAGGTCACCAATGGGGGTACAAGTGTTGTAAGCAGTTTATGCGAAACAGTTATTGCACAGGTGCTGCTGGAGTTGAGGCTGCCGAGGCTGCAATACATCTCATGGAGCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

180

Amino Acids

20.42

Weight (kDa)

5.3

Isoelectric Point (pI)

37.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Slu7 PF11708 1 - 141 1.5e-47 Pre-mRNA splicing Prp18-interacting factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 183, 192
AciI CCGC 1 cut(s) 359
AclWI GGATC 2 cut(s) 23, 344
AfaI GTAC 1 cut(s) 449
AgsI TTSAA 1 cut(s) 317
AjuI GAANNNNNNNTTGG 2 cut(s) 159, 191
AleI CACNNNNGTG 2 cut(s) 408, 410
AloI GAACNNNNNNTCC 2 cut(s) 126, 158
AluBI AGCT 6 cut(s) 83, 158, 170, 260, 286, 329
AluI AGCT 6 cut(s) 83, 158, 170, 260, 286, 329
AlwI GGATC 2 cut(s) 23, 344
AlwNI CAGNNNCTG 1 cut(s) 494
ApeKI GCWGC 5 cut(s) 155, 257, 494, 509, 518
Asp700I GAANNNNTTC 2 cut(s) 273, 360
AsuHPI GGTGA 1 cut(s) 428
AxyI CCTNAGG 1 cut(s) 279
BaeI ACNNNNGTAYC 2 cut(s) 439, 472
BbvI GCAGC 5 cut(s) 167, 269, 481, 496, 505
BccI CCATC 1 cut(s) 169
BfaI CTAG 2 cut(s) 239, 261
BisI GCNGC 5 cut(s) 156, 258, 495, 510, 519
BlsI GCNGC 5 cut(s) 157, 259, 496, 511, 520
BmiI GGNNCC 2 cut(s) 363, 537
BmrI ACTGGG 1 cut(s) 302
BmsI GCATC 1 cut(s) 13
BmuI ACTGGG 1 cut(s) 302
BpmI CTGGAG 2 cut(s) 107, 519
BsaAI YACGTR 1 cut(s) 49
BsaBI GATNNNNATC 1 cut(s) 27
BsaJI CCNNGG 2 cut(s) 365, 513
BsaXI ACNNNNNCTCC 2 cut(s) 45, 75
Bse1I ACTGG 2 cut(s) 79, 297
Bse21I CCTNAGG 1 cut(s) 279
Bse3DI GCAATG 1 cut(s) 259
Bse8I GATNNNNATC 1 cut(s) 27
BseDI CCNNGG 2 cut(s) 365, 513
BseGI GGATG 1 cut(s) 161
BseJI GATNNNNATC 1 cut(s) 27
BseMI GCAATG 1 cut(s) 259
BseMII CTCAG 1 cut(s) 162
BseNI ACTGG 2 cut(s) 79, 297
BseRI GAGGAG 1 cut(s) 382
BseXI GCAGC 5 cut(s) 167, 269, 481, 496, 505
BseYI CCCAGC 1 cut(s) 329
Bsh1285I CGRYCG 1 cut(s) 325
BsiEI CGRYCG 1 cut(s) 325
BslFI GGGAC 2 cut(s) 147, 309
BsmFI GGGAC 2 cut(s) 147, 309
Bsp143I GATC 3 cut(s) 28, 322, 336
BspACI CCGC 1 cut(s) 359
BspCNI CTCAG 1 cut(s) 163
BspLI GGNNCC 2 cut(s) 363, 537
BspPI GGATC 2 cut(s) 23, 344
BsrDI GCAATG 1 cut(s) 259
BsrI ACTGG 2 cut(s) 79, 297
BssECI CCNNGG 2 cut(s) 365, 513
BssMI GATC 3 cut(s) 28, 322, 336
Bst4CI ACNGT 1 cut(s) 479
BstAPI GCANNNNNTGC 1 cut(s) 469
BstBAI YACGTR 1 cut(s) 49
BstC8I GCNNGC 2 cut(s) 38, 153
BstDEI CTNAG 2 cut(s) 171, 279
BstEII GGTNACC 1 cut(s) 434
BstF5I GGATG 1 cut(s) 161
BstKTI GATC 3 cut(s) 31, 325, 339
BstMBI GATC 3 cut(s) 28, 322, 336
BstMCI CGRYCG 1 cut(s) 325
BstMWI GCNNNNNNNGC 4 cut(s) 119, 469, 515, 518
BstNSI RCATGY 1 cut(s) 151
BstPI GGTNACC 1 cut(s) 434
BstV1I GCAGC 5 cut(s) 167, 269, 481, 496, 505
BstX2I RGATCY 1 cut(s) 28
BstYI RGATCY 1 cut(s) 28
Bsu36I CCTNAGG 1 cut(s) 279
BtsCI GGATG 1 cut(s) 161
BtsIMutI CAGTG 1 cut(s) 72
Cac8I GCNNGC 2 cut(s) 38, 153
CaiI CAGNNNCTG 1 cut(s) 494
Csp6I GTAC 1 cut(s) 448
CviAII CATG 3 cut(s) 110, 148, 532
CviJI RGCY 9 cut(s) 83, 158, 170, 260, 286, 329, 509, 518, 538
CviKI_1 RGCY 9 cut(s) 83, 158, 170, 260, 286, 329, 509, 518, 538
CviQI GTAC 1 cut(s) 448
DdeI CTNAG 2 cut(s) 171, 279
DpnI GATC 3 cut(s) 30, 324, 338
DpnII GATC 3 cut(s) 28, 322, 336
Eco81I CCTNAGG 1 cut(s) 279
Eco91I GGTNACC 1 cut(s) 434
EcoO65I GGTNACC 1 cut(s) 434
FaeI CATG 3 cut(s) 113, 151, 535
FaiI YATR 8 cut(s) 111, 149, 183, 192, 249, 378, 470, 533
FaqI GGGAC 2 cut(s) 147, 309
FatI CATG 3 cut(s) 109, 147, 531
Fnu4HI GCNGC 5 cut(s) 156, 258, 495, 510, 519
FokI GGATG 1 cut(s) 148
Fsp4HI GCNGC 5 cut(s) 156, 258, 495, 510, 519
FspBI CTAG 2 cut(s) 239, 261
GluI GCNGC 5 cut(s) 156, 258, 495, 510, 519
GsaI CCCAGC 1 cut(s) 333
GsuI CTGGAG 2 cut(s) 107, 519
Hin1II CATG 3 cut(s) 113, 151, 535
HphI GGTGA 1 cut(s) 428
Hpy166II GTNNAC 1 cut(s) 145
Hpy188I TCNGA 2 cut(s) 33, 366
Hpy188III TCNNGA 3 cut(s) 20, 86, 239
Hpy8I GTNNAC 1 cut(s) 145
HpyCH4III ACNGT 1 cut(s) 479
HpyCH4IV ACGT 1 cut(s) 48
HpyCH4V TGCA 7 cut(s) 26, 151, 231, 257, 380, 486, 521
HpyF10VI GCNNNNNNNGC 4 cut(s) 119, 469, 515, 518
HpyF3I CTNAG 2 cut(s) 171, 279
HpySE526I ACGT 1 cut(s) 48
Hsp92II CATG 3 cut(s) 113, 151, 535
Kzo9I GATC 3 cut(s) 28, 322, 336
LmnI GCTCC 4 cut(s) 163, 283, 369, 535
LpnPI CCDG 8 cut(s) 33, 60, 71, 79, 278, 315, 474, 483
Lsp1109I GCAGC 5 cut(s) 167, 269, 481, 496, 505
LweI GCATC 1 cut(s) 13
MaeI CTAG 2 cut(s) 239, 261
MaeII ACGT 1 cut(s) 48
MaeIII GTNAC 1 cut(s) 434
MalI GATC 3 cut(s) 30, 324, 338
MboI GATC 3 cut(s) 28, 322, 336
MboII GAAGA 3 cut(s) 281, 365, 395
MflI RGATCY 1 cut(s) 28
MluCI AATT 2 cut(s) 187, 226
MnlI CCTC 6 cut(s) 123, 327, 343, 360, 499, 508
MroXI GAANNNNTTC 2 cut(s) 273, 360
MseI TTAA 2 cut(s) 201, 541
MslI CAYNNNNRTG 2 cut(s) 408, 410
MwoI GCNNNNNNNGC 4 cut(s) 119, 469, 515, 518
NdeII GATC 3 cut(s) 28, 322, 336
NlaIII CATG 3 cut(s) 113, 151, 535
NlaIV GGNNCC 2 cut(s) 363, 537
NmeAIII GCCGAG 1 cut(s) 538
NmuCI GTSAC 1 cut(s) 434
NspI RCATGY 1 cut(s) 151
OliI CACNNNNGTG 2 cut(s) 408, 410
PdmI GAANNNNTTC 2 cut(s) 273, 360
PkrI GCNGC 5 cut(s) 157, 259, 496, 511, 520
Ple19I CGATCG 1 cut(s) 325
Ppu21I YACGTR 1 cut(s) 49
PsiI TTATAA 2 cut(s) 183, 192
PspEI GGTNACC 1 cut(s) 434
PspFI CCCAGC 1 cut(s) 329
PspN4I GGNNCC 2 cut(s) 363, 537
PstNI CAGNNNCTG 1 cut(s) 494
PsuI RGATCY 1 cut(s) 28
PvuI CGATCG 1 cut(s) 325
RsaI GTAC 1 cut(s) 449
RsaNI GTAC 1 cut(s) 448
RseI CAYNNNNRTG 2 cut(s) 408, 410
SaqAI TTAA 2 cut(s) 201, 541
SatI GCNGC 5 cut(s) 156, 258, 495, 510, 519
Sau3AI GATC 3 cut(s) 28, 322, 336
SetI ASST 9 cut(s) 51, 85, 160, 172, 262, 288, 331, 436, 493
SfaNI GCATC 1 cut(s) 13
SmiMI CAYNNNNRTG 2 cut(s) 408, 410
Sse9I AATT 2 cut(s) 187, 226
SsiI CCGC 1 cut(s) 359
SspMI CTAG 2 cut(s) 239, 261
TaaI ACNGT 1 cut(s) 479
TaiI ACGT 1 cut(s) 51
TaqI TCGA 1 cut(s) 325
TasI AATT 2 cut(s) 187, 226
Tru1I TTAA 2 cut(s) 201, 541
Tru9I TTAA 2 cut(s) 201, 541
TscAI CASTG 1 cut(s) 79
TseFI GTSAC 1 cut(s) 434
TseI GCWGC 5 cut(s) 155, 257, 494, 509, 518
Tsp45I GTSAC 1 cut(s) 434
TspDTI ATGAA 2 cut(s) 98, 116
TspRI CASTG 1 cut(s) 79
XbaI TCTAGA 1 cut(s) 238
XceI RCATGY 1 cut(s) 151
XmnI GAANNNNTTC 2 cut(s) 273, 360
XspI CTAG 2 cut(s) 239, 261
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.