Rroxscaffold_3G00257680

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005942
Physical Location & Seq
Forward (+)
51992313 .. 51993809
1497 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00257680.1

Sequence Viewer

Length: 723 bp
ATGGGGTCCCGGGTAGTATTAACTGCACGGGATGTGAAGAGGGGGCAAGAAGCTGTCGAAAAGCTCAAGCTTTCTGGCTTGACTGATGTCAACAATGCAGGAGTTGGCGGATTCACAGTGATTGGAGAGAATGTAAAGAACTTAACTTTCGATTATGAACATATGTTTGGTTCAAAAGTTGGAGACCTGAATAAGTTTTTAGAGCAAACATACGAAAGTGCAGAGAAATGTCTGAAAACGAACTACTATGGAATCAAGCAAATGAGCAAAGCACTTATTCGACTTCTGCAAAAGTCTGATTCACCGAAAATAGTCAATGTCTCTTCCAACGTAGGACAGTTAAAGCTTCTTACGCATCAGAAAGCAAGGGATGAGCTAGGAGATGTCGAAGGCCTCACCGAAGAGAAAGTGGACAAGGTTGTCGAGGATTTTCTGAAGGATGTGAAAGAGAATTTGGTCGAAGCTAATGGCTGGCCTATAAACTTCTCTGCTTACAATGTGTCCAAGGCAGCCTTGAATGCTTACACCAGAGTTCTGGCAAAGAATCACCCCAATATTGCAATCAATGCAGTTGGTCCCGGCTACACTAGGACTGACTTCGGCTTCAATCTTGGTGGCCACTCTGTTGAAGAAGGTGCAAAGGGTCCTGTTAAGCTGGCCTTGAGCCCTGAAGGTGGACCTTCTGGCCTCTATTTTGACCAGCTTGAAGTCTCACCCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

240

Amino Acids

26.18

Weight (kDa)

6.45

Isoelectric Point (pI)

26.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short_C2 PF13561 161 - 203 7.5e-06 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 419
AciI CCGC 1 cut(s) 108
AcoI YGGCCR 1 cut(s) 616
AcsI RAATTY 1 cut(s) 451
AcuI CTGAAG 2 cut(s) 455, 690
AfiI CCNNNNNNNGG 1 cut(s) 674
AgsI TTSAA 5 cut(s) 174, 517, 607, 629, 707
AjuI GAANNNNNNNTTGG 4 cut(s) 150, 182, 437, 469
AluBI AGCT 8 cut(s) 53, 64, 70, 346, 376, 464, 655, 703
AluI AGCT 8 cut(s) 53, 64, 70, 346, 376, 464, 655, 703
Alw26I GTCTC 3 cut(s) 177, 325, 715
Ama87I CYCGRG 1 cut(s) 9
AoxI GGCC 5 cut(s) 391, 473, 616, 657, 685
ApeKI GCWGC 1 cut(s) 509
ApoI RAATTY 1 cut(s) 451
AspS9I GGNCC 4 cut(s) 6, 575, 644, 677
AsuC2I CCSGG 3 cut(s) 10, 11, 579
AsuHPI GGTGA 4 cut(s) 294, 388, 539, 705
AvaI CYCGRG 1 cut(s) 9
AvaII GGWCC 4 cut(s) 6, 575, 644, 677
BalI TGGCCA 1 cut(s) 618
BanII GRGCYC 1 cut(s) 668
BbvI GCAGC 1 cut(s) 521
BcnI CCSGG 3 cut(s) 10, 11, 579
BcoDI GTCTC 3 cut(s) 177, 325, 715
BfaI CTAG 2 cut(s) 377, 588
BisI GCNGC 1 cut(s) 510
BlsI GCNGC 1 cut(s) 511
Bme1390I CCNGG 3 cut(s) 10, 11, 579
Bme18I GGWCC 4 cut(s) 6, 575, 644, 677
BmeT110I CYCGRG 1 cut(s) 9
BmgT120I GGNCC 4 cut(s) 6, 575, 644, 677
BmiI GGNNCC 4 cut(s) 7, 8, 577, 645
BmrFI CCNGG 3 cut(s) 10, 11, 579
BmsI GCATC 1 cut(s) 364
BoxI GACNNNNGTC 1 cut(s) 86
BpuEI CTTGAG 2 cut(s) 50, 682
BpuMI CCSGG 3 cut(s) 10, 11, 579
BsaI GGTCTC 1 cut(s) 177
BsaJI CCNNGG 2 cut(s) 9, 504
BsaXI ACNNNNNCTCC 2 cut(s) 117, 147
Bsc4I CCNNNNNNNGG 1 cut(s) 674
BseDI CCNNGG 2 cut(s) 9, 504
BseGI GGATG 3 cut(s) 37, 376, 445
BseLI CCNNNNNNNGG 1 cut(s) 674
BseXI GCAGC 1 cut(s) 521
BsgI GTGCAG 2 cut(s) 9, 240
BshFI GGCC 5 cut(s) 393, 475, 618, 659, 687
BsiHKCI CYCGRG 1 cut(s) 9
BsiSI CCGG 2 cut(s) 10, 579
BslFI GGGAC 1 cut(s) 561
BslI CCNNNNNNNGG 1 cut(s) 674
BsmAI GTCTC 3 cut(s) 177, 325, 715
BsmFI GGGAC 1 cut(s) 561
BsmI GAATGC 1 cut(s) 523
BsnI GGCC 5 cut(s) 393, 475, 618, 659, 687
Bso31I GGTCTC 1 cut(s) 177
BsoBI CYCGRG 1 cut(s) 9
Bsp1286I GDGCHC 1 cut(s) 668
BspACI CCGC 1 cut(s) 108
BspANI GGCC 5 cut(s) 393, 475, 618, 659, 687
BspLI GGNNCC 4 cut(s) 7, 8, 577, 645
BspTNI GGTCTC 1 cut(s) 177
BssECI CCNNGG 2 cut(s) 9, 504
BssT1I CCWWGG 1 cut(s) 504
Bst4CI ACNGT 2 cut(s) 118, 339
Bst6I CTCTTC 3 cut(s) 32, 328, 396
BstAPI GCANNNNNTGC 1 cut(s) 566
BstC8I GCNNGC 2 cut(s) 473, 657
BstF5I GGATG 3 cut(s) 37, 376, 445
BstMAI GTCTC 3 cut(s) 177, 325, 715
BstMWI GCNNNNNNNGC 3 cut(s) 352, 518, 566
BstPAI GACNNNNGTC 1 cut(s) 86
BstSCI CCNGG 3 cut(s) 8, 9, 577
BstV1I GCAGC 1 cut(s) 521
BstXI CCANNNNNNTGG 1 cut(s) 535
BsuRI GGCC 5 cut(s) 393, 475, 618, 659, 687
BtsCI GGATG 3 cut(s) 37, 376, 445
BtsIMutI CAGTG 1 cut(s) 123
Cac8I GCNNGC 2 cut(s) 473, 657
Cfr13I GGNCC 4 cut(s) 6, 575, 644, 677
Cfr9I CCCGGG 1 cut(s) 9
CspCI CAANNNNNGTGG 2 cut(s) 595, 630
DrdI GACNNNNNNGTC 1 cut(s) 419
DseDI GACNNNNNNGTC 1 cut(s) 419
EaeI YGGCCR 1 cut(s) 616
Eam1104I CTCTTC 3 cut(s) 32, 328, 396
EarI CTCTTC 3 cut(s) 32, 328, 396
EciI GGCGGA 1 cut(s) 123
Eco130I CCWWGG 1 cut(s) 504
Eco147I AGGCCT 1 cut(s) 393
Eco24I GRGCYC 1 cut(s) 668
Eco31I GGTCTC 1 cut(s) 177
Eco47I GGWCC 4 cut(s) 6, 575, 644, 677
Eco57I CTGAAG 2 cut(s) 455, 690
Eco88I CYCGRG 1 cut(s) 9
EcoO109I RGGNCCY 2 cut(s) 6, 644
EcoT14I CCWWGG 1 cut(s) 504
EcoT38I GRGCYC 1 cut(s) 668
ErhI CCWWGG 1 cut(s) 504
FaiI YATR 6 cut(s) 156, 162, 164, 211, 249, 479
FalI AAGNNNNNCTT 4 cut(s) 497, 529, 644, 676
FaqI GGGAC 1 cut(s) 561
FauNDI CATATG 1 cut(s) 162
Fnu4HI GCNGC 1 cut(s) 510
FokI GGATG 3 cut(s) 44, 383, 452
FriOI GRGCYC 1 cut(s) 668
Fsp4HI GCNGC 1 cut(s) 510
FspBI CTAG 2 cut(s) 377, 588
GluI GCNGC 1 cut(s) 510
HaeIII GGCC 5 cut(s) 393, 475, 618, 659, 687
HapII CCGG 2 cut(s) 10, 579
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HindIII AAGCTT 2 cut(s) 68, 344
HinfI GANTC 4 cut(s) 111, 252, 299, 544
HpaII CCGG 2 cut(s) 10, 579
HphI GGTGA 4 cut(s) 294, 388, 539, 705
Hpy166II GTNNAC 3 cut(s) 91, 412, 677
Hpy188I TCNGA 5 cut(s) 234, 298, 360, 435, 722
Hpy8I GTNNAC 3 cut(s) 91, 412, 677
HpyAV CCTTC 5 cut(s) 383, 430, 626, 665, 690
HpyCH4III ACNGT 2 cut(s) 118, 339
HpyCH4IV ACGT 1 cut(s) 330
HpyCH4V TGCA 7 cut(s) 26, 98, 221, 289, 560, 569, 638
HpyF10VI GCNNNNNNNGC 3 cut(s) 352, 518, 566
HpySE526I ACGT 1 cut(s) 330
KflI GGGWCCC 1 cut(s) 6
Lsp1109I GCAGC 1 cut(s) 521
LweI GCATC 1 cut(s) 364
MaeI CTAG 2 cut(s) 377, 588
MaeII ACGT 1 cut(s) 330
MboII GAAGA 4 cut(s) 49, 315, 413, 641
MhlI GDGCHC 1 cut(s) 668
MlsI TGGCCA 1 cut(s) 618
MluCI AATT 1 cut(s) 451
MluNI TGGCCA 1 cut(s) 618
MmeI TCCRAC 2 cut(s) 160, 351
MnlI CCTC 4 cut(s) 33, 404, 418, 698
Mox20I TGGCCA 1 cut(s) 618
MscI TGGCCA 1 cut(s) 618
MseI TTAA 4 cut(s) 20, 143, 341, 651
Msp20I TGGCCA 1 cut(s) 618
MspI CCGG 2 cut(s) 10, 579
MspR9I CCNGG 3 cut(s) 10, 11, 579
Mva1269I GAATGC 1 cut(s) 523
MwoI GCNNNNNNNGC 3 cut(s) 352, 518, 566
NciI CCSGG 3 cut(s) 10, 11, 579
NdeI CATATG 1 cut(s) 162
NlaIV GGNNCC 4 cut(s) 7, 8, 577, 645
PceI AGGCCT 1 cut(s) 393
PctI GAATGC 1 cut(s) 523
PfeI GAWTC 4 cut(s) 111, 252, 299, 544
PkrI GCNGC 1 cut(s) 511
PpuMI RGGWCCY 2 cut(s) 6, 644
PshAI GACNNNNGTC 1 cut(s) 86
Psp5II RGGWCCY 2 cut(s) 6, 644
PspN4I GGNNCC 4 cut(s) 7, 8, 577, 645
PspPI GGNCC 4 cut(s) 6, 575, 644, 677
PspPPI RGGWCCY 2 cut(s) 6, 644
SaqAI TTAA 4 cut(s) 20, 143, 341, 651
SatI GCNGC 1 cut(s) 510
Sau96I GGNCC 4 cut(s) 6, 575, 644, 677
ScrFI CCNGG 3 cut(s) 10, 11, 579
SduI GDGCHC 1 cut(s) 668
SfaNI GCATC 1 cut(s) 364
SinI GGWCC 4 cut(s) 6, 575, 644, 677
SmaI CCCGGG 1 cut(s) 11
SmlI CTYRAG 2 cut(s) 65, 661
SmoI CTYRAG 2 cut(s) 65, 661
Sse9I AATT 1 cut(s) 451
SseBI AGGCCT 1 cut(s) 393
SsiI CCGC 1 cut(s) 108
SspI AATATT 1 cut(s) 556
SspMI CTAG 2 cut(s) 377, 588
StuI AGGCCT 1 cut(s) 393
StyD4I CCNGG 3 cut(s) 8, 9, 577
StyI CCWWGG 1 cut(s) 504
TaaI ACNGT 2 cut(s) 118, 339
TaiI ACGT 1 cut(s) 333
TaqI TCGA 6 cut(s) 57, 150, 280, 387, 423, 459
TasI AATT 1 cut(s) 451
TfiI GAWTC 4 cut(s) 111, 252, 299, 544
Tru1I TTAA 4 cut(s) 20, 143, 341, 651
Tru9I TTAA 4 cut(s) 20, 143, 341, 651
TscAI CASTG 1 cut(s) 123
TseI GCWGC 1 cut(s) 509
TspDTI ATGAA 1 cut(s) 171
TspMI CCCGGG 1 cut(s) 9
TspRI CASTG 1 cut(s) 123
VpaK11BI GGWCC 4 cut(s) 6, 575, 644, 677
XapI RAATTY 1 cut(s) 451
XmaI CCCGGG 1 cut(s) 9
XspI CTAG 2 cut(s) 377, 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.