Rroxscaffold_3G00257650

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0005942
Physical Location & Seq
Forward (+)
51971734 .. 51974678
2945 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00257650.1

Sequence Viewer

Length: 918 bp
ATGGCGGAAACAACCATTGATTCTCAGTCAAAGAGAATTTGTCTTGTCACCGGGGCCAACAAAGGGATTGGATTTGAGATATGCAGGCAACTAGCTTCTAATGGGATCCTGGTAGTACTAACTGCAAGGGATGCGAAAAGCGGACAAGAAGCTGTCGAAAAGCTCAAGCTTTCTGGCTTGACTGATGTAATTTTTCATCAACTAGATGTAACCGATGCAACTAGCATTGCTTCTTTAGCAGATTTCATTGAATCCCAGTTCGGGAAGCTTGACATATTGGTCAACAATGCAGCGATTAACGGAGTCACGGTGATCGGAGGGAATTATGCAAAGAACATAAATTTTGATTATGAGCATATGTTTGGTTCAAAAGCTGGAGACTTGAACAAGCATCTAGAGCAAACATACGAAAGTGCAGAGAAATGTCTGAAAACGAACTACTATGGAATCAAACAAATGAGCAAAGCAACTATTCGACTTCTGCAAAAATCTGGTTTACCTAAAATGGTCAATGTCTCTTCCAACGGAGGACAGTTAAAGCATGTTACGCATCAGAAAGCAAGGGATGAGCTAGGAGATGTTCAAGGCCTCACCGAAGAGAAAGTGGACAAGGTTGTTGAGGAGTTTCTGAAGGATGCGAAAGAGAATTTGGTCGAAGCTAATGGCTGGCCTATAAACTTCTCTGCTTACGTAGTGTCCAAGGCAGCCATGAATGCTTACACCAGAGTTCTGGCAAAGAGCCACCCCGAAATTGCAATCAATGCAGTTTCTCCTGGCTACACTAGCACTGACATGACCTTCAACGTTGGCGGGCACTCTGTTGAAGATGGTGCAAAGGGTCCTGTTAAGCTGGCCTTGAGCCCTGAAGGTGGACCTTCTGGCCTCTATTTTGACCAGCTTGAAGTCTCATTGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

305

Amino Acids

32.94

Weight (kDa)

5.85

Isoelectric Point (pI)

30.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 13 - 180 7.4e-25 short chain dehydrogenase
KR PF08659 15 - 99 1.2e-06 KR domain
Epimerase PF01370 15 - 103 5.6e-06 NAD dependent epimerase/dehydratase family
adh_short_C2 PF13561 18 - 107 2.6e-16 Enoyl-(Acyl carrier protein) reductase
adh_short PF00106 225 - 267 1.4e-06 short chain dehydrogenase
adh_short_C2 PF13561 226 - 271 1.9e-06 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 278
AciI CCGC 3 cut(s) 5, 141, 810
AclI AACGTT 1 cut(s) 804
AclWI GGATC 2 cut(s) 100, 113
AcsI RAATTY 3 cut(s) 36, 340, 646
AcuI CTGAAG 2 cut(s) 650, 885
AfaI GTAC 1 cut(s) 117
AfiI CCNNNNNNNGG 3 cut(s) 63, 261, 869
AgsI TTSAA 7 cut(s) 251, 369, 385, 584, 802, 824, 902
AjnI CCWGG 2 cut(s) 108, 772
AjuI GAANNNNNNNTTGG 2 cut(s) 632, 664
Alw26I GTCTC 3 cut(s) 372, 520, 910
AlwI GGATC 2 cut(s) 100, 113
AoxI GGCC 5 cut(s) 54, 586, 668, 852, 880
ApeKI GCWGC 2 cut(s) 290, 704
ApoI RAATTY 3 cut(s) 36, 340, 646
AspS9I GGNCC 3 cut(s) 54, 839, 872
AsuC2I CCSGG 1 cut(s) 52
AsuHPI GGTGA 3 cut(s) 40, 322, 583
AvaII GGWCC 2 cut(s) 839, 872
BaeGI GKGCMC 1 cut(s) 816
BamHI GGATCC 1 cut(s) 105
BanII GRGCYC 1 cut(s) 863
BbvI GCAGC 2 cut(s) 302, 716
BccI CCATC 1 cut(s) 821
BciT130I CCWGG 2 cut(s) 110, 774
BcnI CCSGG 1 cut(s) 52
BcoDI GTCTC 3 cut(s) 372, 520, 910
BfaI CTAG 6 cut(s) 92, 203, 222, 395, 572, 783
BisI GCNGC 2 cut(s) 291, 705
BlsI GCNGC 2 cut(s) 292, 706
BmcAI AGTACT 1 cut(s) 117
Bme1390I CCNGG 3 cut(s) 52, 110, 774
Bme18I GGWCC 2 cut(s) 839, 872
BmgT120I GGNCC 3 cut(s) 54, 839, 872
BmiI GGNNCC 3 cut(s) 55, 107, 840
BmrFI CCNGG 3 cut(s) 52, 110, 774
BmrI ACTGGG 1 cut(s) 250
BmsI GCATC 5 cut(s) 121, 205, 400, 559, 625
BmuI ACTGGG 1 cut(s) 250
BpmI CTGGAG 1 cut(s) 396
BpuEI CTTGAG 2 cut(s) 149, 877
BpuMI CCSGG 1 cut(s) 52
BsaAI YACGTR 1 cut(s) 691
BsaJI CCNNGG 2 cut(s) 51, 699
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 3 cut(s) 63, 261, 869
Bse1I ACTGG 1 cut(s) 256
Bse3DI GCAATG 1 cut(s) 225
BseBI CCWGG 2 cut(s) 110, 774
BseDI CCNNGG 2 cut(s) 51, 699
BseGI GGATG 3 cut(s) 136, 571, 640
BseLI CCNNNNNNNGG 3 cut(s) 63, 261, 869
BseMI GCAATG 1 cut(s) 225
BseMII CTCAG 1 cut(s) 38
BseNI ACTGG 1 cut(s) 256
BseRI GAGGAG 1 cut(s) 635
BseSI GKGCMC 1 cut(s) 816
BseXI GCAGC 2 cut(s) 302, 716
BsgI GTGCAG 1 cut(s) 435
BshFI GGCC 5 cut(s) 56, 588, 670, 854, 882
BsiSI CCGG 1 cut(s) 51
BslI CCNNNNNNNGG 3 cut(s) 63, 261, 869
BsmAI GTCTC 3 cut(s) 372, 520, 910
BsmI GAATGC 1 cut(s) 718
BsnI GGCC 5 cut(s) 56, 588, 670, 854, 882
Bsp1286I GDGCHC 2 cut(s) 816, 863
Bsp143I GATC 2 cut(s) 105, 312
BspACI CCGC 3 cut(s) 5, 141, 810
BspANI GGCC 5 cut(s) 56, 588, 670, 854, 882
BspCNI CTCAG 1 cut(s) 37
BspLI GGNNCC 3 cut(s) 55, 107, 840
BspPI GGATC 2 cut(s) 100, 113
BsrDI GCAATG 1 cut(s) 225
BsrI ACTGG 1 cut(s) 256
BssECI CCNNGG 2 cut(s) 51, 699
BssMI GATC 2 cut(s) 105, 312
BssT1I CCWWGG 1 cut(s) 699
Bst2UI CCWGG 2 cut(s) 110, 774
Bst4CI ACNGT 2 cut(s) 310, 534
Bst6I CTCTTC 2 cut(s) 523, 591
BstAPI GCANNNNNTGC 2 cut(s) 131, 761
BstBAI YACGTR 1 cut(s) 691
BstC8I GCNNGC 4 cut(s) 86, 668, 812, 852
BstDEI CTNAG 1 cut(s) 24
BstF5I GGATG 3 cut(s) 136, 571, 640
BstKTI GATC 2 cut(s) 108, 315
BstMAI GTCTC 3 cut(s) 372, 520, 910
BstMBI GATC 2 cut(s) 105, 312
BstMWI GCNNNNNNNGC 7 cut(s) 131, 236, 397, 547, 713, 761, 783
BstNI CCWGG 2 cut(s) 110, 774
BstNSI RCATGY 1 cut(s) 545
BstSCI CCNGG 3 cut(s) 50, 108, 772
BstSLI GKGCMC 1 cut(s) 816
BstSNI TACGTA 1 cut(s) 691
BstV1I GCAGC 2 cut(s) 302, 716
BstX2I RGATCY 1 cut(s) 105
BstXI CCANNNNNNTGG 1 cut(s) 730
BstYI RGATCY 1 cut(s) 105
BsuRI GGCC 5 cut(s) 56, 588, 670, 854, 882
BtsCI GGATG 3 cut(s) 136, 571, 640
BtsIMutI CAGTG 1 cut(s) 786
Cac8I GCNNGC 4 cut(s) 86, 668, 812, 852
Cfr13I GGNCC 3 cut(s) 54, 839, 872
Csp6I GTAC 1 cut(s) 116
CviAII CATG 3 cut(s) 542, 709, 793
CviQI GTAC 1 cut(s) 116
DdeI CTNAG 1 cut(s) 24
DpnI GATC 2 cut(s) 107, 314
DpnII GATC 2 cut(s) 105, 312
DrdI GACNNNNNNGTC 1 cut(s) 278
DseDI GACNNNNNNGTC 1 cut(s) 278
Eam1104I CTCTTC 2 cut(s) 523, 591
EarI CTCTTC 2 cut(s) 523, 591
EciI GGCGGA 1 cut(s) 20
Eco105I TACGTA 1 cut(s) 691
Eco130I CCWWGG 1 cut(s) 699
Eco147I AGGCCT 1 cut(s) 588
Eco24I GRGCYC 1 cut(s) 863
Eco47I GGWCC 2 cut(s) 839, 872
Eco57I CTGAAG 2 cut(s) 650, 885
EcoO109I RGGNCCY 1 cut(s) 839
EcoRII CCWGG 2 cut(s) 108, 772
EcoT14I CCWWGG 1 cut(s) 699
EcoT38I GRGCYC 1 cut(s) 863
ErhI CCWWGG 1 cut(s) 699
FaeI CATG 3 cut(s) 545, 712, 796
FalI AAGNNNNNCTT 2 cut(s) 839, 871
FatI CATG 3 cut(s) 541, 708, 792
FauI CCCGC 1 cut(s) 803
FauNDI CATATG 1 cut(s) 357
Fnu4HI GCNGC 2 cut(s) 291, 705
FokI GGATG 3 cut(s) 143, 578, 647
FriOI GRGCYC 1 cut(s) 863
Fsp4HI GCNGC 2 cut(s) 291, 705
FspBI CTAG 6 cut(s) 92, 203, 222, 395, 572, 783
GluI GCNGC 2 cut(s) 291, 705
GsuI CTGGAG 1 cut(s) 396
HaeIII GGCC 5 cut(s) 56, 588, 670, 854, 882
HapII CCGG 1 cut(s) 51
Hin1II CATG 3 cut(s) 545, 712, 796
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HindIII AAGCTT 2 cut(s) 167, 266
HinfI GANTC 4 cut(s) 20, 251, 303, 447
HpaII CCGG 1 cut(s) 51
HphI GGTGA 3 cut(s) 40, 322, 583
Hpy166II GTNNAC 4 cut(s) 283, 497, 607, 872
Hpy188I TCNGA 4 cut(s) 317, 429, 555, 630
Hpy188III TCNNGA 2 cut(s) 262, 395
Hpy8I GTNNAC 4 cut(s) 283, 497, 607, 872
HpyAV CCTTC 4 cut(s) 625, 808, 860, 885
HpyCH4III ACNGT 2 cut(s) 310, 534
HpyCH4IV ACGT 2 cut(s) 690, 804
HpyF10VI GCNNNNNNNGC 7 cut(s) 131, 236, 397, 547, 713, 761, 783
HpyF3I CTNAG 1 cut(s) 24
HpySE526I ACGT 2 cut(s) 690, 804
Hsp92II CATG 3 cut(s) 545, 712, 796
Kzo9I GATC 2 cut(s) 105, 312
Lsp1109I GCAGC 2 cut(s) 302, 716
LweI GCATC 5 cut(s) 121, 205, 400, 559, 625
MaeI CTAG 6 cut(s) 92, 203, 222, 395, 572, 783
MaeII ACGT 2 cut(s) 690, 804
MaeIII GTNAC 4 cut(s) 46, 208, 304, 544
MalI GATC 2 cut(s) 107, 314
MboI GATC 2 cut(s) 105, 312
MboII GAAGA 3 cut(s) 510, 608, 836
MflI RGATCY 1 cut(s) 105
MhlI GDGCHC 2 cut(s) 816, 863
MluCI AATT 6 cut(s) 36, 189, 322, 340, 646, 750
MlyI GAGTC 1 cut(s) 312
MmeI TCCRAC 1 cut(s) 546
MnlI CCTC 5 cut(s) 311, 521, 599, 613, 893
MseI TTAA 3 cut(s) 297, 536, 846
MslI CAYNNNNRTG 1 cut(s) 791
MspI CCGG 1 cut(s) 51
MspR9I CCNGG 3 cut(s) 52, 110, 774
Mva1269I GAATGC 1 cut(s) 718
MvaI CCWGG 2 cut(s) 110, 774
MwoI GCNNNNNNNGC 7 cut(s) 131, 236, 397, 547, 713, 761, 783
NciI CCSGG 1 cut(s) 52
NdeI CATATG 1 cut(s) 357
NdeII GATC 2 cut(s) 105, 312
NlaIII CATG 3 cut(s) 545, 712, 796
NlaIV GGNNCC 3 cut(s) 55, 107, 840
NmuCI GTSAC 2 cut(s) 46, 304
NspI RCATGY 1 cut(s) 545
PceI AGGCCT 1 cut(s) 588
PctI GAATGC 1 cut(s) 718
PfeI GAWTC 3 cut(s) 20, 251, 447
PkrI GCNGC 2 cut(s) 292, 706
PleI GAGTC 1 cut(s) 311
PpsI GAGTC 1 cut(s) 311
Ppu21I YACGTR 1 cut(s) 691
PpuMI RGGWCCY 1 cut(s) 839
Psp1406I AACGTT 1 cut(s) 804
Psp5II RGGWCCY 1 cut(s) 839
Psp6I CCWGG 2 cut(s) 108, 772
PspGI CCWGG 2 cut(s) 108, 772
PspN4I GGNNCC 3 cut(s) 55, 107, 840
PspPI GGNCC 3 cut(s) 54, 839, 872
PspPPI RGGWCCY 1 cut(s) 839
PsuI RGATCY 1 cut(s) 105
RsaI GTAC 1 cut(s) 117
RsaNI GTAC 1 cut(s) 116
RseI CAYNNNNRTG 1 cut(s) 791
SaqAI TTAA 3 cut(s) 297, 536, 846
SatI GCNGC 2 cut(s) 291, 705
Sau3AI GATC 2 cut(s) 105, 312
Sau96I GGNCC 3 cut(s) 54, 839, 872
ScaI AGTACT 1 cut(s) 117
SchI GAGTC 1 cut(s) 312
ScrFI CCNGG 3 cut(s) 52, 110, 774
SduI GDGCHC 2 cut(s) 816, 863
SfaNI GCATC 5 cut(s) 121, 205, 400, 559, 625
SinI GGWCC 2 cut(s) 839, 872
SmiMI CAYNNNNRTG 1 cut(s) 791
SmlI CTYRAG 2 cut(s) 164, 856
SmoI CTYRAG 2 cut(s) 164, 856
SnaBI TACGTA 1 cut(s) 691
Sse9I AATT 6 cut(s) 36, 189, 322, 340, 646, 750
SseBI AGGCCT 1 cut(s) 588
SsiI CCGC 3 cut(s) 5, 141, 810
SspMI CTAG 6 cut(s) 92, 203, 222, 395, 572, 783
StuI AGGCCT 1 cut(s) 588
StyD4I CCNGG 3 cut(s) 50, 108, 772
StyI CCWWGG 1 cut(s) 699
TaaI ACNGT 2 cut(s) 310, 534
TaiI ACGT 2 cut(s) 693, 807
TaqI TCGA 3 cut(s) 156, 475, 654
TasI AATT 6 cut(s) 36, 189, 322, 340, 646, 750
TatI WGTACW 1 cut(s) 115
TfiI GAWTC 3 cut(s) 20, 251, 447
Tru1I TTAA 3 cut(s) 297, 536, 846
Tru9I TTAA 3 cut(s) 297, 536, 846
TscAI CASTG 1 cut(s) 793
TseFI GTSAC 2 cut(s) 46, 304
TseI GCWGC 2 cut(s) 290, 704
Tsp45I GTSAC 2 cut(s) 46, 304
TspDTI ATGAA 3 cut(s) 185, 235, 725
TspGWI ACGGA 2 cut(s) 315, 540
TspRI CASTG 1 cut(s) 793
VpaK11BI GGWCC 2 cut(s) 839, 872
XapI RAATTY 3 cut(s) 36, 340, 646
XbaI TCTAGA 1 cut(s) 394
XceI RCATGY 1 cut(s) 545
XspI CTAG 6 cut(s) 92, 203, 222, 395, 572, 783
ZrmI AGTACT 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.