RchiOBHm_Chr7g0198561

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0005942
Physical Location & Seq
Reverse (-)
16484633 .. 16486444
1812 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ17765

Sequence Viewer

Length: 756 bp
ATGTTACAACCAATTTGTGTTGTCACCGGGGCCAACAAAGGGATTGGATTTGAGATATGCAGGCAACTAGCTTCTAATGGGATCCTGGTAGTATTAACTGCAAGAGATGTGAAGAGCGGACAAGAAGCTGTCGAAAAGCTCAAGCTTTCTGGCTTGACTGATGTAATTTTTCATCAACTAGATGTAACCGATGCAACTAGCATTGCTTCTTTAGCAGATTTCATCGAATCCCAGTTCGGGAAGCTTGACATATTGATGTTTGGTTCAAAAGCTGGAGACTTGAAAAAGCATCTAGAGCAAACATACGAAAGGGCAGAGAAATGTCTGAAAACGAACTACTATGGAATCAAGCAAATGAGCAAAGCAACTATTCGACTTCTGCAAAAATCTGGTTCACCTAAAATGGTCAATGTCTCTTCCATCGGAGGACAGTTAAAGAAAGTGGACAAGGTTGTTGAGGAGTTTCTGAAGGATGCGAAAGAGAATTTGGTTGAAGCTAATGACTGGCCTATAAACTTCTCTGCTTACGTAGTGTCCAAGGCAGCCATGAATGCTTACACCAGAGTTCTGGCAAAGAGCCACCCCGAAATTGCAATCAATGCAGTTAGTCCTGGCTACACTAGCACTGACATGACCTTCAACGTTGGCGGGCACTCTGTTGAAGAAGGTGCAAAGGGTCCTGTTAAGCTGGCCTTGAGTCCTGAAGGTGGACCTTCTAGCCTCTATTTTGACCAGCTTGAAGTCTCATCGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

251

Amino Acids

27.17

Weight (kDa)

6.91

Isoelectric Point (pI)

29.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 6 - 146 3.3e-25 short chain dehydrogenase
KR PF08659 7 - 145 7.3e-08 KR domain
Epimerase PF01370 7 - 194 5.4e-06 NAD dependent epimerase/dehydratase family
adh_short_C2 PF13561 10 - 145 6.1e-20 Enoyl-(Acyl carrier protein) reductase
adh_short PF00106 171 - 213 8.9e-07 short chain dehydrogenase
adh_short_C2 PF13561 172 - 217 1.8e-06 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 117
AciI CCGC 2 cut(s) 117, 648
AclI AACGTT 1 cut(s) 642
AclWI GGATC 2 cut(s) 76, 89
AcsI RAATTY 1 cut(s) 484
AcuI CTGAAG 2 cut(s) 488, 723
AfiI CCNNNNNNNGG 3 cut(s) 39, 237, 707
AgsI TTSAA 6 cut(s) 267, 283, 494, 640, 662, 740
AjnI CCWGG 2 cut(s) 84, 610
AjuI GAANNNNNNNTTGG 2 cut(s) 470, 502
AluBI AGCT 9 cut(s) 71, 128, 139, 145, 244, 272, 497, 688, 736
AluI AGCT 9 cut(s) 71, 128, 139, 145, 244, 272, 497, 688, 736
Alw26I GTCTC 3 cut(s) 270, 418, 748
AlwI GGATC 2 cut(s) 76, 89
AoxI GGCC 3 cut(s) 30, 506, 690
ApeKI GCWGC 1 cut(s) 542
ApoI RAATTY 1 cut(s) 484
AspS9I GGNCC 3 cut(s) 30, 677, 710
AsuC2I CCSGG 1 cut(s) 28
AsuHPI GGTGA 2 cut(s) 16, 387
AvaII GGWCC 2 cut(s) 677, 710
BaeGI GKGCMC 1 cut(s) 654
BamHI GGATCC 1 cut(s) 81
BbvI GCAGC 1 cut(s) 554
BccI CCATC 1 cut(s) 428
BciT130I CCWGG 2 cut(s) 86, 612
BcnI CCSGG 1 cut(s) 28
BcoDI GTCTC 3 cut(s) 270, 418, 748
BfaI CTAG 6 cut(s) 68, 179, 198, 293, 621, 717
BisI GCNGC 1 cut(s) 543
BlsI GCNGC 1 cut(s) 544
Bme1390I CCNGG 3 cut(s) 28, 86, 612
Bme18I GGWCC 2 cut(s) 677, 710
BmgT120I GGNCC 3 cut(s) 30, 677, 710
BmiI GGNNCC 3 cut(s) 31, 83, 678
BmrFI CCNGG 3 cut(s) 28, 86, 612
BmrI ACTGGG 1 cut(s) 226
BmsI GCATC 3 cut(s) 181, 298, 463
BmuI ACTGGG 1 cut(s) 226
BpmI CTGGAG 1 cut(s) 294
BpuEI CTTGAG 2 cut(s) 125, 715
BpuMI CCSGG 1 cut(s) 28
BsaAI YACGTR 1 cut(s) 529
BsaJI CCNNGG 2 cut(s) 27, 537
Bsc4I CCNNNNNNNGG 3 cut(s) 39, 237, 707
Bse1I ACTGG 2 cut(s) 232, 509
Bse3DI GCAATG 1 cut(s) 201
BseBI CCWGG 2 cut(s) 86, 612
BseDI CCNNGG 2 cut(s) 27, 537
BseGI GGATG 1 cut(s) 478
BseLI CCNNNNNNNGG 3 cut(s) 39, 237, 707
BseMI GCAATG 1 cut(s) 201
BseNI ACTGG 2 cut(s) 232, 509
BseRI GAGGAG 1 cut(s) 473
BseSI GKGCMC 1 cut(s) 654
BseXI GCAGC 1 cut(s) 554
BshFI GGCC 3 cut(s) 32, 508, 692
BsiSI CCGG 1 cut(s) 27
BslI CCNNNNNNNGG 3 cut(s) 39, 237, 707
BsmAI GTCTC 3 cut(s) 270, 418, 748
BsmI GAATGC 1 cut(s) 556
BsnI GGCC 3 cut(s) 32, 508, 692
Bsp1286I GDGCHC 1 cut(s) 654
Bsp143I GATC 1 cut(s) 81
BspACI CCGC 2 cut(s) 117, 648
BspANI GGCC 3 cut(s) 32, 508, 692
BspLI GGNNCC 3 cut(s) 31, 83, 678
BspPI GGATC 2 cut(s) 76, 89
BspQI GCTCTTC 1 cut(s) 107
BsrBI CCGCTC 1 cut(s) 117
BsrDI GCAATG 1 cut(s) 201
BsrI ACTGG 2 cut(s) 232, 509
BssECI CCNNGG 2 cut(s) 27, 537
BssMI GATC 1 cut(s) 81
BssT1I CCWWGG 1 cut(s) 537
Bst2UI CCWGG 2 cut(s) 86, 612
Bst4CI ACNGT 1 cut(s) 432
Bst6I CTCTTC 2 cut(s) 107, 421
BstAPI GCANNNNNTGC 1 cut(s) 599
BstBAI YACGTR 1 cut(s) 529
BstC8I GCNNGC 3 cut(s) 62, 650, 690
BstF5I GGATG 1 cut(s) 478
BstKTI GATC 1 cut(s) 84
BstMAI GTCTC 3 cut(s) 270, 418, 748
BstMBI GATC 1 cut(s) 81
BstMWI GCNNNNNNNGC 5 cut(s) 212, 295, 551, 599, 621
BstNI CCWGG 2 cut(s) 86, 612
BstSCI CCNGG 3 cut(s) 26, 84, 610
BstSLI GKGCMC 1 cut(s) 654
BstSNI TACGTA 1 cut(s) 529
BstV1I GCAGC 1 cut(s) 554
BstX2I RGATCY 1 cut(s) 81
BstXI CCANNNNNNTGG 1 cut(s) 568
BstYI RGATCY 1 cut(s) 81
BsuRI GGCC 3 cut(s) 32, 508, 692
BtsCI GGATG 1 cut(s) 478
BtsIMutI CAGTG 1 cut(s) 624
Cac8I GCNNGC 3 cut(s) 62, 650, 690
Cfr13I GGNCC 3 cut(s) 30, 677, 710
CviAII CATG 2 cut(s) 547, 631
DpnI GATC 1 cut(s) 83
DpnII GATC 1 cut(s) 81
Eam1104I CTCTTC 2 cut(s) 107, 421
EarI CTCTTC 2 cut(s) 107, 421
Eco105I TACGTA 1 cut(s) 529
Eco130I CCWWGG 1 cut(s) 537
Eco47I GGWCC 2 cut(s) 677, 710
Eco57I CTGAAG 2 cut(s) 488, 723
EcoO109I RGGNCCY 1 cut(s) 677
EcoRII CCWGG 2 cut(s) 84, 610
EcoT14I CCWWGG 1 cut(s) 537
ErhI CCWWGG 1 cut(s) 537
FaeI CATG 2 cut(s) 550, 634
FaiI YATR 7 cut(s) 58, 251, 304, 342, 512, 548, 632
FalI AAGNNNNNCTT 2 cut(s) 677, 709
FatI CATG 2 cut(s) 546, 630
FauI CCCGC 1 cut(s) 641
Fnu4HI GCNGC 1 cut(s) 543
FokI GGATG 1 cut(s) 485
Fsp4HI GCNGC 1 cut(s) 543
FspBI CTAG 6 cut(s) 68, 179, 198, 293, 621, 717
GluI GCNGC 1 cut(s) 543
GsuI CTGGAG 1 cut(s) 294
HaeIII GGCC 3 cut(s) 32, 508, 692
HapII CCGG 1 cut(s) 27
Hin1II CATG 2 cut(s) 550, 634
HindIII AAGCTT 2 cut(s) 143, 242
HinfI GANTC 3 cut(s) 227, 345, 697
HpaII CCGG 1 cut(s) 27
HphI GGTGA 2 cut(s) 16, 387
Hpy166II GTNNAC 3 cut(s) 395, 445, 710
Hpy188I TCNGA 3 cut(s) 327, 425, 468
Hpy188III TCNNGA 3 cut(s) 238, 293, 701
Hpy8I GTNNAC 3 cut(s) 395, 445, 710
HpyAV CCTTC 5 cut(s) 463, 646, 659, 698, 723
HpyCH4III ACNGT 1 cut(s) 432
HpyCH4IV ACGT 2 cut(s) 528, 642
HpyCH4V TGCA 7 cut(s) 60, 101, 194, 382, 593, 602, 671
HpyF10VI GCNNNNNNNGC 5 cut(s) 212, 295, 551, 599, 621
HpySE526I ACGT 2 cut(s) 528, 642
Hsp92II CATG 2 cut(s) 550, 634
Kzo9I GATC 1 cut(s) 81
LguI GCTCTTC 1 cut(s) 107
Lsp1109I GCAGC 1 cut(s) 554
LweI GCATC 3 cut(s) 181, 298, 463
MaeI CTAG 6 cut(s) 68, 179, 198, 293, 621, 717
MaeII ACGT 2 cut(s) 528, 642
MaeIII GTNAC 3 cut(s) 3, 22, 184
MalI GATC 1 cut(s) 83
MbiI CCGCTC 1 cut(s) 117
MboI GATC 1 cut(s) 81
MboII GAAGA 3 cut(s) 124, 408, 674
MflI RGATCY 1 cut(s) 81
MhlI GDGCHC 1 cut(s) 654
MluCI AATT 4 cut(s) 12, 165, 484, 588
MlyI GAGTC 1 cut(s) 706
MnlI CCTC 3 cut(s) 419, 451, 731
MseI TTAA 3 cut(s) 95, 434, 684
MslI CAYNNNNRTG 2 cut(s) 254, 629
MspI CCGG 1 cut(s) 27
MspR9I CCNGG 3 cut(s) 28, 86, 612
Mva1269I GAATGC 1 cut(s) 556
MvaI CCWGG 2 cut(s) 86, 612
MwoI GCNNNNNNNGC 5 cut(s) 212, 295, 551, 599, 621
NciI CCSGG 1 cut(s) 28
NdeII GATC 1 cut(s) 81
NlaIII CATG 2 cut(s) 550, 634
NlaIV GGNNCC 3 cut(s) 31, 83, 678
NmuCI GTSAC 1 cut(s) 22
PciSI GCTCTTC 1 cut(s) 107
PctI GAATGC 1 cut(s) 556
PfeI GAWTC 2 cut(s) 227, 345
PkrI GCNGC 1 cut(s) 544
PleI GAGTC 1 cut(s) 705
PpsI GAGTC 1 cut(s) 705
Ppu21I YACGTR 1 cut(s) 529
PpuMI RGGWCCY 1 cut(s) 677
Psp1406I AACGTT 1 cut(s) 642
Psp5II RGGWCCY 1 cut(s) 677
Psp6I CCWGG 2 cut(s) 84, 610
PspGI CCWGG 2 cut(s) 84, 610
PspN4I GGNNCC 3 cut(s) 31, 83, 678
PspPI GGNCC 3 cut(s) 30, 677, 710
PspPPI RGGWCCY 1 cut(s) 677
PsuI RGATCY 1 cut(s) 81
RseI CAYNNNNRTG 2 cut(s) 254, 629
SapI GCTCTTC 1 cut(s) 107
SaqAI TTAA 3 cut(s) 95, 434, 684
SatI GCNGC 1 cut(s) 543
Sau3AI GATC 1 cut(s) 81
Sau96I GGNCC 3 cut(s) 30, 677, 710
SchI GAGTC 1 cut(s) 706
ScrFI CCNGG 3 cut(s) 28, 86, 612
SduI GDGCHC 1 cut(s) 654
SfaNI GCATC 3 cut(s) 181, 298, 463
SinI GGWCC 2 cut(s) 677, 710
SmiMI CAYNNNNRTG 2 cut(s) 254, 629
SmlI CTYRAG 2 cut(s) 140, 694
SmoI CTYRAG 2 cut(s) 140, 694
SnaBI TACGTA 1 cut(s) 529
Sse9I AATT 4 cut(s) 12, 165, 484, 588
SsiI CCGC 2 cut(s) 117, 648
SspMI CTAG 6 cut(s) 68, 179, 198, 293, 621, 717
StyD4I CCNGG 3 cut(s) 26, 84, 610
StyI CCWWGG 1 cut(s) 537
TaaI ACNGT 1 cut(s) 432
TaiI ACGT 2 cut(s) 531, 645
TaqI TCGA 3 cut(s) 132, 225, 373
TasI AATT 4 cut(s) 12, 165, 484, 588
TfiI GAWTC 2 cut(s) 227, 345
Tru1I TTAA 3 cut(s) 95, 434, 684
Tru9I TTAA 3 cut(s) 95, 434, 684
TscAI CASTG 1 cut(s) 631
TseFI GTSAC 1 cut(s) 22
TseI GCWGC 1 cut(s) 542
Tsp45I GTSAC 1 cut(s) 22
TspDTI ATGAA 3 cut(s) 161, 211, 563
TspRI CASTG 1 cut(s) 631
VpaK11BI GGWCC 2 cut(s) 677, 710
XapI RAATTY 1 cut(s) 484
XbaI TCTAGA 1 cut(s) 292
XspI CTAG 6 cut(s) 68, 179, 198, 293, 621, 717
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.