RLG00000003865

Salutaridine reductase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0005942
Physical Location & Seq
Forward (+)
53715496 .. 53718382
2887 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003865

Sequence Viewer

Length: 915 bp
ATGGCCGAAACAACCATCAAGTCTCAGACAAAGAGAGTTTCTGTTGTCACGGGGGCCAACAAAGGGATTGGATTTGAGATATGCAGGCAATTAGCTTCTAATGGGGTCCGGGTAGTATTAACTGCACGGGATGTGAAGAGGGGGCAAGAAGCTATCGAAAAGCTCAAGCTTTCTGGCTTGACTGATGTAATTTTTCATCAGCTAGATGTAACCGATGCAACTAGCATTGCTTCTTTAGCAGATTTCATCGAAACCCAGTTCGGGAAGCTTGACATATTGGTCAACAATGCAGGAGTTGGCGGATTCACAGTGATTGGAGAGAATGTAAAGAACTTAACTTTCGATTATGAACATATGTTTGGTTCAAAAGTTGGAGACCTGAATAAGTTTCTAGAGCAAACATACGAAAGTGCAGAGAAATGTCTGAAAACGAACTATTATGGAATCAAGCAAATGAGCAAAGCACTTATTCGACTTCTGCAAAAGTCTGATTCACCGAAAATAGTCAATGTCTCTTCCAACGGAGGACAGTTAAAGCTTCTTACGCATCAGAAAGCAAGGGATGAGCTAGGAGATGTCGAAGGCCTCACCGAAGAGAAAGTGGACAAGGTTGTCGAGGATTTTCTGAAGGATGTGAAAGAGAATTTGGTCGAAGCTAATGGCTGGCCTATAAACTTTTCTGCTTACAATGTGTCCAAGGCAGCCTTGAATGCTTACACCAGAGTTCTGGCAAAGAATCACCCCAATATTGCAATCAATGCAGTTGGTCCCGGCTACACTAGGACTGACTTGGGCTTCAATCTTGGTGGCCACTCTGTTGAAGAAGGTGCAAAGGGTCCTGTTAAGCTGGCCTTGAGCCCTGAAGGTGGACCTTCTGGCCTCTATTTTGACCAGCTTGAAGTCTCACCCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

305

Amino Acids

33.03

Weight (kDa)

6.25

Isoelectric Point (pI)

26.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 13 - 178 6.4e-29 short chain dehydrogenase
KR PF08659 15 - 124 1.1e-08 KR domain
adh_short_C2 PF13561 18 - 102 2.1e-16 Enoyl-(Acyl carrier protein) reductase
adh_short_C2 PF13561 225 - 269 6.4e-06 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 278, 611
AciI CCGC 1 cut(s) 300
AcoI YGGCCR 2 cut(s) 3, 808
AcsI RAATTY 1 cut(s) 643
AcuI CTGAAG 2 cut(s) 647, 882
AfiI CCNNNNNNNGG 3 cut(s) 63, 261, 866
AgsI TTSAA 5 cut(s) 366, 709, 799, 821, 899
AjuI GAANNNNNNNTTGG 4 cut(s) 342, 374, 629, 661
Alw26I GTCTC 4 cut(s) 27, 369, 517, 907
AoxI GGCC 7 cut(s) 3, 54, 583, 665, 808, 849, 877
ApeKI GCWGC 1 cut(s) 701
ApoI RAATTY 1 cut(s) 643
AspS9I GGNCC 5 cut(s) 54, 106, 767, 836, 869
AsuC2I CCSGG 2 cut(s) 110, 771
AsuHPI GGTGA 4 cut(s) 486, 580, 731, 897
AvaII GGWCC 4 cut(s) 106, 767, 836, 869
BalI TGGCCA 1 cut(s) 810
BanII GRGCYC 1 cut(s) 860
BbvI GCAGC 1 cut(s) 713
BccI CCATC 1 cut(s) 23
BcnI CCSGG 2 cut(s) 110, 771
BcoDI GTCTC 4 cut(s) 27, 369, 517, 907
BfaI CTAG 5 cut(s) 203, 222, 392, 569, 780
BisI GCNGC 1 cut(s) 702
BlsI GCNGC 1 cut(s) 703
Bme1390I CCNGG 2 cut(s) 110, 771
Bme18I GGWCC 4 cut(s) 106, 767, 836, 869
BmgT120I GGNCC 5 cut(s) 54, 106, 767, 836, 869
BmiI GGNNCC 4 cut(s) 55, 107, 769, 837
BmrFI CCNGG 2 cut(s) 110, 771
BmrI ACTGGG 1 cut(s) 250
BmsI GCATC 2 cut(s) 205, 556
BmuI ACTGGG 1 cut(s) 250
BpuEI CTTGAG 2 cut(s) 149, 874
BpuMI CCSGG 2 cut(s) 110, 771
BsaI GGTCTC 1 cut(s) 369
BsaJI CCNNGG 1 cut(s) 696
BsaXI ACNNNNNCTCC 2 cut(s) 309, 339
Bsc4I CCNNNNNNNGG 3 cut(s) 63, 261, 866
Bse1I ACTGG 1 cut(s) 256
Bse3DI GCAATG 1 cut(s) 225
BseDI CCNNGG 1 cut(s) 696
BseGI GGATG 3 cut(s) 136, 568, 637
BseLI CCNNNNNNNGG 3 cut(s) 63, 261, 866
BseMI GCAATG 1 cut(s) 225
BseMII CTCAG 1 cut(s) 38
BseNI ACTGG 1 cut(s) 256
BseXI GCAGC 1 cut(s) 713
BsgI GTGCAG 2 cut(s) 108, 432
BshFI GGCC 7 cut(s) 5, 56, 585, 667, 810, 851, 879
BsiSI CCGG 2 cut(s) 109, 771
BslFI GGGAC 1 cut(s) 753
BslI CCNNNNNNNGG 3 cut(s) 63, 261, 866
BsmAI GTCTC 4 cut(s) 27, 369, 517, 907
BsmFI GGGAC 1 cut(s) 753
BsmI GAATGC 1 cut(s) 715
BsnI GGCC 7 cut(s) 5, 56, 585, 667, 810, 851, 879
Bso31I GGTCTC 1 cut(s) 369
Bsp1286I GDGCHC 1 cut(s) 860
BspACI CCGC 1 cut(s) 300
BspANI GGCC 7 cut(s) 5, 56, 585, 667, 810, 851, 879
BspCNI CTCAG 1 cut(s) 37
BspLI GGNNCC 4 cut(s) 55, 107, 769, 837
BspTNI GGTCTC 1 cut(s) 369
BsrDI GCAATG 1 cut(s) 225
BsrI ACTGG 1 cut(s) 256
BssECI CCNNGG 1 cut(s) 696
BssT1I CCWWGG 1 cut(s) 696
Bst4CI ACNGT 2 cut(s) 310, 531
Bst6I CTCTTC 3 cut(s) 131, 520, 588
BstAPI GCANNNNNTGC 1 cut(s) 758
BstC8I GCNNGC 3 cut(s) 86, 665, 849
BstDEI CTNAG 1 cut(s) 24
BstF5I GGATG 3 cut(s) 136, 568, 637
BstMAI GTCTC 4 cut(s) 27, 369, 517, 907
BstMWI GCNNNNNNNGC 4 cut(s) 236, 544, 710, 758
BstSCI CCNGG 2 cut(s) 108, 769
BstV1I GCAGC 1 cut(s) 713
BstXI CCANNNNNNTGG 1 cut(s) 727
BsuRI GGCC 7 cut(s) 5, 56, 585, 667, 810, 851, 879
BtsCI GGATG 3 cut(s) 136, 568, 637
BtsIMutI CAGTG 1 cut(s) 315
Cac8I GCNNGC 3 cut(s) 86, 665, 849
Cfr13I GGNCC 5 cut(s) 54, 106, 767, 836, 869
CspCI CAANNNNNGTGG 2 cut(s) 787, 822
DdeI CTNAG 1 cut(s) 24
DrdI GACNNNNNNGTC 2 cut(s) 278, 611
DseDI GACNNNNNNGTC 2 cut(s) 278, 611
EaeI YGGCCR 2 cut(s) 3, 808
Eam1104I CTCTTC 3 cut(s) 131, 520, 588
EarI CTCTTC 3 cut(s) 131, 520, 588
EciI GGCGGA 1 cut(s) 315
Eco130I CCWWGG 1 cut(s) 696
Eco147I AGGCCT 1 cut(s) 585
Eco24I GRGCYC 1 cut(s) 860
Eco31I GGTCTC 1 cut(s) 369
Eco47I GGWCC 4 cut(s) 106, 767, 836, 869
Eco57I CTGAAG 2 cut(s) 647, 882
EcoO109I RGGNCCY 1 cut(s) 836
EcoT14I CCWWGG 1 cut(s) 696
EcoT38I GRGCYC 1 cut(s) 860
ErhI CCWWGG 1 cut(s) 696
FaiI YATR 8 cut(s) 82, 275, 348, 354, 356, 403, 441, 671
FalI AAGNNNNNCTT 4 cut(s) 689, 721, 836, 868
FaqI GGGAC 1 cut(s) 753
FauNDI CATATG 1 cut(s) 354
Fnu4HI GCNGC 1 cut(s) 702
FokI GGATG 3 cut(s) 143, 575, 644
FriOI GRGCYC 1 cut(s) 860
Fsp4HI GCNGC 1 cut(s) 702
FspBI CTAG 5 cut(s) 203, 222, 392, 569, 780
GluI GCNGC 1 cut(s) 702
HaeIII GGCC 7 cut(s) 5, 56, 585, 667, 810, 851, 879
HapII CCGG 2 cut(s) 109, 771
HincII GTYRAC 1 cut(s) 283
HindII GTYRAC 1 cut(s) 283
HindIII AAGCTT 3 cut(s) 167, 266, 536
HinfI GANTC 4 cut(s) 303, 444, 491, 736
HpaII CCGG 2 cut(s) 109, 771
HphI GGTGA 4 cut(s) 486, 580, 731, 897
Hpy166II GTNNAC 3 cut(s) 283, 604, 869
Hpy188I TCNGA 6 cut(s) 27, 426, 490, 552, 627, 914
Hpy188III TCNNGA 2 cut(s) 262, 392
Hpy8I GTNNAC 3 cut(s) 283, 604, 869
HpyAV CCTTC 5 cut(s) 575, 622, 818, 857, 882
HpyCH4III ACNGT 2 cut(s) 310, 531
HpyCH4V TGCA 9 cut(s) 84, 125, 218, 290, 413, 481, 752, 761, 830
HpyF10VI GCNNNNNNNGC 4 cut(s) 236, 544, 710, 758
HpyF3I CTNAG 1 cut(s) 24
Lsp1109I GCAGC 1 cut(s) 713
LweI GCATC 2 cut(s) 205, 556
MaeI CTAG 5 cut(s) 203, 222, 392, 569, 780
MaeIII GTNAC 2 cut(s) 46, 208
MboII GAAGA 4 cut(s) 148, 507, 605, 833
MhlI GDGCHC 1 cut(s) 860
MlsI TGGCCA 1 cut(s) 810
MluCI AATT 3 cut(s) 89, 189, 643
MluNI TGGCCA 1 cut(s) 810
MmeI TCCRAC 2 cut(s) 352, 543
MnlI CCTC 5 cut(s) 132, 518, 596, 610, 890
Mox20I TGGCCA 1 cut(s) 810
MscI TGGCCA 1 cut(s) 810
MseI TTAA 4 cut(s) 119, 335, 533, 843
Msp20I TGGCCA 1 cut(s) 810
MspI CCGG 2 cut(s) 109, 771
MspR9I CCNGG 2 cut(s) 110, 771
Mva1269I GAATGC 1 cut(s) 715
MwoI GCNNNNNNNGC 4 cut(s) 236, 544, 710, 758
NciI CCSGG 2 cut(s) 110, 771
NdeI CATATG 1 cut(s) 354
NlaIV GGNNCC 4 cut(s) 55, 107, 769, 837
NmuCI GTSAC 1 cut(s) 46
PceI AGGCCT 1 cut(s) 585
PctI GAATGC 1 cut(s) 715
PfeI GAWTC 4 cut(s) 303, 444, 491, 736
PkrI GCNGC 1 cut(s) 703
PpuMI RGGWCCY 1 cut(s) 836
Psp5II RGGWCCY 1 cut(s) 836
PspN4I GGNNCC 4 cut(s) 55, 107, 769, 837
PspPI GGNCC 5 cut(s) 54, 106, 767, 836, 869
PspPPI RGGWCCY 1 cut(s) 836
SaqAI TTAA 4 cut(s) 119, 335, 533, 843
SatI GCNGC 1 cut(s) 702
Sau96I GGNCC 5 cut(s) 54, 106, 767, 836, 869
ScrFI CCNGG 2 cut(s) 110, 771
SduI GDGCHC 1 cut(s) 860
SfaNI GCATC 2 cut(s) 205, 556
SinI GGWCC 4 cut(s) 106, 767, 836, 869
SmlI CTYRAG 2 cut(s) 164, 853
SmoI CTYRAG 2 cut(s) 164, 853
Sse9I AATT 3 cut(s) 89, 189, 643
SseBI AGGCCT 1 cut(s) 585
SsiI CCGC 1 cut(s) 300
SspI AATATT 1 cut(s) 748
SspMI CTAG 5 cut(s) 203, 222, 392, 569, 780
StuI AGGCCT 1 cut(s) 585
StyD4I CCNGG 2 cut(s) 108, 769
StyI CCWWGG 1 cut(s) 696
TaaI ACNGT 2 cut(s) 310, 531
TaqI TCGA 7 cut(s) 156, 249, 342, 472, 579, 615, 651
TasI AATT 3 cut(s) 89, 189, 643
TfiI GAWTC 4 cut(s) 303, 444, 491, 736
Tru1I TTAA 4 cut(s) 119, 335, 533, 843
Tru9I TTAA 4 cut(s) 119, 335, 533, 843
TscAI CASTG 1 cut(s) 315
TseFI GTSAC 1 cut(s) 46
TseI GCWGC 1 cut(s) 701
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 3 cut(s) 185, 235, 363
TspGWI ACGGA 1 cut(s) 537
TspRI CASTG 1 cut(s) 315
VpaK11BI GGWCC 4 cut(s) 106, 767, 836, 869
XapI RAATTY 1 cut(s) 643
XbaI TCTAGA 1 cut(s) 391
XspI CTAG 5 cut(s) 203, 222, 392, 569, 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.