RchiOBHm_Chr7g0200881

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0007089
Physical Location & Seq
Forward (+)
18584623 .. 18585156
534 bp
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UTR
Exon/CDS
Intron
PRQ17982

Sequence Viewer

Length: 534 bp
ATGCTGTCTCTGGCAACATGTGCGTCGACTCGAGTCTCAAATGAGCTCGGAGCAAACAGGGCAGGACCTGCATACCAATCTGCATTTGTGTCTCTGGCAGTGAGTATGGTCTCTGGTTGTTTAGGTGGATTGGTGATGGTAGCAGCCAGGGGAAGTTGGGGGTCGTTGTTTAGCCACGATAAGGGGATTGTAAGAGGTGTGAAGAAGATGATGCTGCTCATGGCTTTGGTGGAGGTTGTGAATTTCCCATTAGCTGCTTGCGGAGGGATTGTTCGAGGAACAGCGAGGCCATGGTTGGCTATGTATGCAAATCTTGGTGGATTCTACCTTCTGGCTTTGCCCTTGGGAGTTGTTTTAGCCTTCGAGGTTAAACTCGGGCTGAGTGGATTATTGATAGGATTCTTGATTGGGATGGTTGCTTGCTTGATTTTGTTGCTGATCTTTGTTTTAAGAATCAATTGGGATGAAGAAGCTGTTGGGAAATTAATCCCCCGTCACGCAAGTACTACTAGTAGAGATGGAAAGAAGAAATAA

Protein Analysis

177

Amino Acids

18.67

Weight (kDa)

9.83

Isoelectric Point (pI)

29.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 1 - 117 1.1e-18 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 76
AccI GTMKAC 1 cut(s) 26
AciI CCGC 1 cut(s) 261
AcsI RAATTY 1 cut(s) 241
AfaI GTAC 1 cut(s) 505
AfiI CCNNNNNNNGG 1 cut(s) 181
AflIII ACRYGT 1 cut(s) 17
AhlI ACTAGT 1 cut(s) 509
AjnI CCWGG 1 cut(s) 146
AjuI GAANNNNNNNTTGG 2 cut(s) 459, 491
AloI GAACNNNNNNTCC 2 cut(s) 255, 287
AluBI AGCT 3 cut(s) 46, 254, 473
AluI AGCT 3 cut(s) 46, 254, 473
Alw21I GWGCWC 1 cut(s) 48
Alw26I GTCTC 4 cut(s) 12, 40, 96, 115
AlwNI CAGNNNCTG 1 cut(s) 68
Ama87I CYCGRG 2 cut(s) 30, 374
AoxI GGCC 1 cut(s) 287
ApeKI GCWGC 3 cut(s) 143, 214, 254
ApoI RAATTY 1 cut(s) 241
AseI ATTAAT 1 cut(s) 485
AspS9I GGNCC 1 cut(s) 65
AsuHPI GGTGA 1 cut(s) 145
AvaI CYCGRG 2 cut(s) 30, 374
AvaII GGWCC 1 cut(s) 65
BanII GRGCYC 1 cut(s) 48
Bbv12I GWGCWC 1 cut(s) 48
BbvI GCAGC 3 cut(s) 155, 201, 241
BccI CCATC 3 cut(s) 130, 406, 512
BciT130I CCWGG 1 cut(s) 148
BcoDI GTCTC 4 cut(s) 12, 40, 96, 115
BcuI ACTAGT 1 cut(s) 509
BfaI CTAG 1 cut(s) 510
BfuAI ACCTGC 1 cut(s) 76
BisI GCNGC 3 cut(s) 144, 215, 255
BlsI GCNGC 3 cut(s) 145, 216, 256
BmcAI AGTACT 1 cut(s) 505
Bme1390I CCNGG 1 cut(s) 148
Bme18I GGWCC 1 cut(s) 65
BmeT110I CYCGRG 2 cut(s) 30, 374
BmgT120I GGNCC 1 cut(s) 65
BmrFI CCNGG 1 cut(s) 148
BmsI GCATC 1 cut(s) 201
BoxI GACNNNNGTC 1 cut(s) 32
BsaI GGTCTC 1 cut(s) 115
BsaJI CCNNGG 3 cut(s) 147, 290, 342
BsaXI ACNNNNNCTCC 2 cut(s) 255, 285
Bsc4I CCNNNNNNNGG 1 cut(s) 181
BseBI CCWGG 1 cut(s) 148
BseDI CCNNGG 3 cut(s) 147, 290, 342
BseGI GGATG 2 cut(s) 417, 469
BseLI CCNNNNNNNGG 1 cut(s) 181
BseMII CTCAG 1 cut(s) 371
BseXI GCAGC 3 cut(s) 155, 201, 241
BshFI GGCC 1 cut(s) 289
BsiHKAI GWGCWC 1 cut(s) 48
BsiHKCI CYCGRG 2 cut(s) 30, 374
BslI CCNNNNNNNGG 1 cut(s) 181
BsmAI GTCTC 4 cut(s) 12, 40, 96, 115
BsnI GGCC 1 cut(s) 289
Bso31I GGTCTC 1 cut(s) 115
BsoBI CYCGRG 2 cut(s) 30, 374
Bsp1286I GDGCHC 1 cut(s) 48
Bsp143I GATC 1 cut(s) 438
Bsp19I CCATGG 1 cut(s) 290
BspACI CCGC 1 cut(s) 261
BspANI GGCC 1 cut(s) 289
BspCNI CTCAG 1 cut(s) 372
BspMI ACCTGC 1 cut(s) 76
BspTNI GGTCTC 1 cut(s) 115
BssECI CCNNGG 3 cut(s) 147, 290, 342
BssMI GATC 1 cut(s) 438
BssT1I CCWWGG 2 cut(s) 290, 342
Bst2UI CCWGG 1 cut(s) 148
BstAPI GCANNNNNTGC 2 cut(s) 20, 68
BstC8I GCNNGC 2 cut(s) 259, 421
BstDEI CTNAG 1 cut(s) 380
BstDSI CCRYGG 1 cut(s) 290
BstF5I GGATG 2 cut(s) 417, 469
BstKTI GATC 1 cut(s) 441
BstMAI GTCTC 4 cut(s) 12, 40, 96, 115
BstMBI GATC 1 cut(s) 438
BstMWI GCNNNNNNNGC 4 cut(s) 20, 59, 68, 305
BstNI CCWGG 1 cut(s) 148
BstNSI RCATGY 1 cut(s) 21
BstPAI GACNNNNGTC 1 cut(s) 32
BstSCI CCNGG 1 cut(s) 146
BstV1I GCAGC 3 cut(s) 155, 201, 241
BsuRI GGCC 1 cut(s) 289
BtgI CCRYGG 1 cut(s) 290
BtsCI GGATG 2 cut(s) 417, 469
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 1 cut(s) 105
BveI ACCTGC 1 cut(s) 76
Cac8I GCNNGC 2 cut(s) 259, 421
CaiI CAGNNNCTG 1 cut(s) 68
Cfr13I GGNCC 1 cut(s) 65
CseI GACGC 1 cut(s) 12
Csp6I GTAC 1 cut(s) 504
CviAII CATG 3 cut(s) 18, 220, 291
CviQI GTAC 1 cut(s) 504
DdeI CTNAG 1 cut(s) 380
DpnI GATC 1 cut(s) 440
DpnII GATC 1 cut(s) 438
Ecl136II GAGCTC 1 cut(s) 46
Eco130I CCWWGG 2 cut(s) 290, 342
Eco24I GRGCYC 1 cut(s) 48
Eco31I GGTCTC 1 cut(s) 115
Eco47I GGWCC 1 cut(s) 65
Eco53kI GAGCTC 1 cut(s) 46
Eco88I CYCGRG 2 cut(s) 30, 374
EcoICRI GAGCTC 1 cut(s) 46
EcoO109I RGGNCCY 1 cut(s) 65
EcoRII CCWGG 1 cut(s) 146
EcoT14I CCWWGG 2 cut(s) 290, 342
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 2 cut(s) 290, 342
FaeI CATG 3 cut(s) 21, 223, 294
FaiI YATR 7 cut(s) 19, 73, 107, 221, 292, 302, 306
FatI CATG 3 cut(s) 17, 219, 290
FblI GTMKAC 1 cut(s) 26
Fnu4HI GCNGC 3 cut(s) 144, 215, 255
FokI GGATG 2 cut(s) 424, 476
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 3 cut(s) 144, 215, 255
FspBI CTAG 1 cut(s) 510
GluI GCNGC 3 cut(s) 144, 215, 255
HaeIII GGCC 1 cut(s) 289
HgaI GACGC 1 cut(s) 12
Hin1II CATG 3 cut(s) 21, 223, 294
HincII GTYRAC 1 cut(s) 27
HindII GTYRAC 1 cut(s) 27
HinfI GANTC 5 cut(s) 28, 33, 321, 399, 453
HphI GGTGA 1 cut(s) 145
Hpy166II GTNNAC 1 cut(s) 27
Hpy188I TCNGA 1 cut(s) 50
Hpy188III TCNNGA 1 cut(s) 403
Hpy8I GTNNAC 1 cut(s) 27
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 2 cut(s) 338, 370
HpyCH4V TGCA 3 cut(s) 71, 83, 308
HpyF10VI GCNNNNNNNGC 4 cut(s) 20, 59, 68, 305
HpyF3I CTNAG 1 cut(s) 380
Hsp92II CATG 3 cut(s) 21, 223, 294
Kzo9I GATC 1 cut(s) 438
LmnI GCTCC 1 cut(s) 50
LpnPI CCDG 8 cut(s) 43, 48, 80, 81, 99, 133, 160, 317
Lsp1109I GCAGC 3 cut(s) 155, 201, 241
LweI GCATC 1 cut(s) 201
MaeI CTAG 1 cut(s) 510
MaeIII GTNAC 1 cut(s) 494
MalI GATC 1 cut(s) 440
MboI GATC 1 cut(s) 438
MboII GAAGA 3 cut(s) 214, 217, 479
MfeI CAATTG 1 cut(s) 457
MhlI GDGCHC 1 cut(s) 48
MluCI AATT 3 cut(s) 241, 457, 482
MlyI GAGTC 2 cut(s) 22, 42
MnlI CCTC 6 cut(s) 188, 226, 257, 269, 279, 358
MseI TTAA 3 cut(s) 369, 449, 485
MspR9I CCNGG 1 cut(s) 148
MunI CAATTG 1 cut(s) 457
MvaI CCWGG 1 cut(s) 148
MwoI GCNNNNNNNGC 4 cut(s) 20, 59, 68, 305
NcoI CCATGG 1 cut(s) 290
NdeII GATC 1 cut(s) 438
NlaIII CATG 3 cut(s) 21, 223, 294
NmuCI GTSAC 1 cut(s) 494
NspI RCATGY 1 cut(s) 21
PaeR7I CTCGAG 1 cut(s) 30
PciI ACATGT 1 cut(s) 17
PfeI GAWTC 3 cut(s) 321, 399, 453
PkrI GCNGC 3 cut(s) 145, 216, 256
PleI GAGTC 2 cut(s) 22, 41
PpsI GAGTC 2 cut(s) 22, 41
PpuMI RGGWCCY 1 cut(s) 65
PscI ACATGT 1 cut(s) 17
PshAI GACNNNNGTC 1 cut(s) 32
PshBI ATTAAT 1 cut(s) 485
Psp124BI GAGCTC 1 cut(s) 48
Psp5II RGGWCCY 1 cut(s) 65
Psp6I CCWGG 1 cut(s) 146
PspGI CCWGG 1 cut(s) 146
PspPI GGNCC 1 cut(s) 65
PspPPI RGGWCCY 1 cut(s) 65
PspXI VCTCGAGB 1 cut(s) 30
PstNI CAGNNNCTG 1 cut(s) 68
RsaI GTAC 1 cut(s) 505
RsaNI GTAC 1 cut(s) 504
SacI GAGCTC 1 cut(s) 48
SalI GTCGAC 1 cut(s) 25
SaqAI TTAA 3 cut(s) 369, 449, 485
SatI GCNGC 3 cut(s) 144, 215, 255
Sau3AI GATC 1 cut(s) 438
Sau96I GGNCC 1 cut(s) 65
ScaI AGTACT 1 cut(s) 505
SchI GAGTC 2 cut(s) 22, 42
ScrFI CCNGG 1 cut(s) 148
SduI GDGCHC 1 cut(s) 48
SetI ASST 9 cut(s) 48, 70, 127, 199, 237, 256, 330, 369, 475
SfaNI GCATC 1 cut(s) 201
Sfr274I CTCGAG 1 cut(s) 30
SinI GGWCC 1 cut(s) 65
SlaI CTCGAG 1 cut(s) 30
SmlI CTYRAG 1 cut(s) 30
SmoI CTYRAG 1 cut(s) 30
SpeI ACTAGT 1 cut(s) 509
Sse9I AATT 3 cut(s) 241, 457, 482
SsiI CCGC 1 cut(s) 261
SspMI CTAG 1 cut(s) 510
SstI GAGCTC 1 cut(s) 48
StyD4I CCNGG 1 cut(s) 146
StyI CCWWGG 2 cut(s) 290, 342
TaqI TCGA 4 cut(s) 26, 31, 274, 363
TasI AATT 3 cut(s) 241, 457, 482
TatI WGTACW 1 cut(s) 503
TfiI GAWTC 3 cut(s) 321, 399, 453
Tru1I TTAA 3 cut(s) 369, 449, 485
Tru9I TTAA 3 cut(s) 369, 449, 485
TscAI CASTG 1 cut(s) 105
TseFI GTSAC 1 cut(s) 494
TseI GCWGC 3 cut(s) 143, 214, 254
Tsp45I GTSAC 1 cut(s) 494
TspDTI ATGAA 1 cut(s) 480
TspRI CASTG 1 cut(s) 105
VpaK11BI GGWCC 1 cut(s) 65
VspI ATTAAT 1 cut(s) 485
XapI RAATTY 1 cut(s) 241
XceI RCATGY 1 cut(s) 21
XhoI CTCGAG 1 cut(s) 30
XmiI GTMKAC 1 cut(s) 26
XspI CTAG 1 cut(s) 510
ZrmI AGTACT 1 cut(s) 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.