Rw7G016390

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0007089
Physical Location & Seq
Forward (+)
16739198 .. 16740548
1351 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G016390.1

Sequence Viewer

Length: 1140 bp
ATGGTTTACCAAAATAGCCATCACAACAGCTTTTCTAGGAAGACTCGGAGAGCTTCAACTGGCTGGTGGCACACTCGGTTTCACTTTCGCTATGTCACCGGCTTCTCTGTCTTGAGTGGTCTCTGTGGTGCCATGGAGCCCCATTGCTTTGTCCCCTCAAAGCCTACTTCGCAAGGCATCACGGTTCCTCCCATGTTCTGCTCAGGTCTCGCTCTTGCCTTTCACATACCAATCAACATAGTGCTTGCAAACGCTAAAGGAATTGAGGGAGTTGCAATGTCTGTCCGGATCACTGACCTTATGGTTGTTATTCTCCTTGCAATTTATGTGTGTGTGATTGAAGTTAAGGCAAAAGAAGGGAGGTGGAAAGAAGGCGGGTGGCTGGACCAAGGTGTTCGGGATTGGATTAGACTGCTCAAGCTTTGTGGACCTTGCTGCCTCACTACCTGCCTAGAATGGTGGTGCTATGAGATTCTGGTCCTGTTAACTGGCCGGCTTGCCAATGCCAAGCAGGCAGTCAGAGTTTTAGCCATCGTACTAAACTTCAACAACTTGCTATACTCGGTGATGCTGTCTCTGGCAACATGTGCGTCGACTCGAGTCTCAAATGAGCTCCGAGCAAACAGGGCAGGACCTGCATACCAATCTGCATTTGTGTCTCTGGCAGTGAGTATGGTCTCTGGTTGTTTAGGTGGATTGGTGATGGTAGCAGCCAGGGGAAGTTGGGGGTCGTTGTTTAGCCACGATAAGGGGATTGTAAGAGGTGTGAAGAAGATGATGCTGCTCATGGCTTTGGTGGAGGTTGTGAATTTCCCATTAGCTGCTTGCGGAGGGATTGTTCGAGGAACAGCGAGGCCATGGTTGGCTATGTATGCAAATCTTGGTGGATTCTACCTTCTGGCTTTGCCCTTGGGAGTTGTTTTAGCCTTCGAGGTTAAACTCGGGCTGAGTGGACTATTGATAGGATTCTTGATTGGGATGGTTGCTTGCTTGATTTTGTTGCTGATCTTTGTTTTAAGAATCAATTGGGATGAAGAAGCGTGCAAAGCACAAAGACTTGCCTCACTTTCCTGTCAAGCACCAGAACTTAACAAACACGAAAACCACAAAAGCGTGGGGAGAGTCGATGCCGAAGTCTAA

Protein Analysis

379

Amino Acids

41.43

Weight (kDa)

9.31

Isoelectric Point (pI)

35.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 144 - 306 3.5e-27 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 455, 643
AccB1I GGYRCC 1 cut(s) 128
AccI GTMKAC 1 cut(s) 593
AccIII TCCGGA 1 cut(s) 285
AciI CCGC 2 cut(s) 375, 828
AclWI GGATC 1 cut(s) 296
AcoI YGGCCR 1 cut(s) 490
AcsI RAATTY 1 cut(s) 808
AfaI GTAC 1 cut(s) 537
AfiI CCNNNNNNNGG 1 cut(s) 748
AflIII ACRYGT 1 cut(s) 584
AgsI TTSAA 3 cut(s) 57, 341, 547
AjnI CCWGG 1 cut(s) 713
AloI GAACNNNNNNTCC 2 cut(s) 822, 854
AluBI AGCT 5 cut(s) 30, 53, 421, 613, 821
AluI AGCT 5 cut(s) 30, 53, 421, 613, 821
Alw21I GWGCWC 1 cut(s) 615
Alw26I GTCTC 6 cut(s) 125, 212, 579, 607, 663, 682
AlwI GGATC 1 cut(s) 296
AlwNI CAGNNNCTG 1 cut(s) 635
Ama87I CYCGRG 2 cut(s) 597, 941
Aor13HI TCCGGA 1 cut(s) 285
AoxI GGCC 2 cut(s) 490, 854
ApeKI GCWGC 4 cut(s) 435, 710, 781, 821
ApoI RAATTY 1 cut(s) 808
AspS9I GGNCC 4 cut(s) 385, 428, 478, 632
AsuHPI GGTGA 3 cut(s) 88, 577, 712
AvaI CYCGRG 2 cut(s) 597, 941
AvaII GGWCC 4 cut(s) 385, 428, 478, 632
BanI GGYRCC 1 cut(s) 128
BanII GRGCYC 2 cut(s) 141, 615
BbsI GAAGAC 1 cut(s) 47
Bbv12I GWGCWC 1 cut(s) 615
BbvI GCAGC 4 cut(s) 422, 722, 768, 808
BccI CCATC 4 cut(s) 27, 539, 697, 973
BciT130I CCWGG 1 cut(s) 715
BcoDI GTCTC 6 cut(s) 125, 212, 579, 607, 663, 682
BfaI CTAG 2 cut(s) 36, 452
BfuAI ACCTGC 2 cut(s) 455, 643
BglI GCCNNNNNGGC 1 cut(s) 512
BisI GCNGC 4 cut(s) 436, 711, 782, 822
BlsI GCNGC 4 cut(s) 437, 712, 783, 823
Bme1390I CCNGG 1 cut(s) 715
Bme18I GGWCC 4 cut(s) 385, 428, 478, 632
BmeT110I CYCGRG 2 cut(s) 597, 941
BmgT120I GGNCC 4 cut(s) 385, 428, 478, 632
BmiI GGNNCC 3 cut(s) 130, 138, 186
BmrFI CCNGG 1 cut(s) 715
BmsI GCATC 4 cut(s) 186, 558, 768, 1117
BoxI GACNNNNGTC 1 cut(s) 599
BpiI GAAGAC 1 cut(s) 47
Bpu10I CCTNAGC 1 cut(s) 202
BpuEI CTTGAG 2 cut(s) 133, 401
BsaI GGTCTC 3 cut(s) 125, 212, 682
BsaJI CCNNGG 5 cut(s) 132, 388, 714, 857, 909
BsaWI WCCGGW 1 cut(s) 285
BsaXI ACNNNNNCTCC 4 cut(s) 172, 202, 822, 852
Bsc4I CCNNNNNNNGG 1 cut(s) 748
Bse118I RCCGGY 2 cut(s) 98, 492
Bse1I ACTGG 2 cut(s) 64, 493
Bse3DI GCAATG 2 cut(s) 142, 282
BseAI TCCGGA 1 cut(s) 285
BseBI CCWGG 1 cut(s) 715
BseDI CCNNGG 5 cut(s) 132, 388, 714, 857, 909
BseGI GGATG 2 cut(s) 984, 1036
BseLI CCNNNNNNNGG 1 cut(s) 748
BseMI GCAATG 2 cut(s) 142, 282
BseMII CTCAG 2 cut(s) 216, 938
BseNI ACTGG 2 cut(s) 64, 493
BseXI GCAGC 4 cut(s) 422, 722, 768, 808
BshFI GGCC 2 cut(s) 492, 856
BshNI GGYRCC 1 cut(s) 128
BsiHKAI GWGCWC 1 cut(s) 615
BsiHKCI CYCGRG 2 cut(s) 597, 941
BsiSI CCGG 3 cut(s) 99, 286, 493
BslFI GGGAC 1 cut(s) 137
BslI CCNNNNNNNGG 1 cut(s) 748
BsmAI GTCTC 6 cut(s) 125, 212, 579, 607, 663, 682
BsmFI GGGAC 1 cut(s) 137
BsnI GGCC 2 cut(s) 492, 856
Bso31I GGTCTC 3 cut(s) 125, 212, 682
BsoBI CYCGRG 2 cut(s) 597, 941
Bsp1286I GDGCHC 2 cut(s) 141, 615
Bsp13I TCCGGA 1 cut(s) 285
Bsp143I GATC 2 cut(s) 288, 1005
Bsp19I CCATGG 2 cut(s) 132, 857
BspACI CCGC 2 cut(s) 375, 828
BspANI GGCC 2 cut(s) 492, 856
BspCNI CTCAG 2 cut(s) 215, 939
BspEI TCCGGA 1 cut(s) 285
BspLI GGNNCC 3 cut(s) 130, 138, 186
BspMI ACCTGC 2 cut(s) 455, 643
BspPI GGATC 1 cut(s) 296
BspT107I GGYRCC 1 cut(s) 128
BspTNI GGTCTC 3 cut(s) 125, 212, 682
BsrDI GCAATG 2 cut(s) 142, 282
BsrFI RCCGGY 2 cut(s) 98, 492
BsrI ACTGG 2 cut(s) 64, 493
BssAI RCCGGY 2 cut(s) 98, 492
BssECI CCNNGG 5 cut(s) 132, 388, 714, 857, 909
BssMI GATC 2 cut(s) 288, 1005
BssT1I CCWWGG 4 cut(s) 132, 388, 857, 909
Bst2UI CCWGG 1 cut(s) 715
Bst4CI ACNGT 1 cut(s) 184
BstAPI GCANNNNNTGC 2 cut(s) 587, 635
BstC8I GCNNGC 7 cut(s) 246, 494, 498, 513, 826, 988, 1042
BstDEI CTNAG 2 cut(s) 202, 947
BstDSI CCRYGG 2 cut(s) 132, 857
BstF5I GGATG 2 cut(s) 984, 1036
BstKTI GATC 2 cut(s) 291, 1008
BstMAI GTCTC 6 cut(s) 125, 212, 579, 607, 663, 682
BstMBI GATC 2 cut(s) 288, 1005
BstMWI GCNNNNNNNGC 7 cut(s) 169, 512, 587, 626, 635, 872, 1046
BstNI CCWGG 1 cut(s) 715
BstNSI RCATGY 1 cut(s) 588
BstPAI GACNNNNGTC 1 cut(s) 599
BstSCI CCNGG 1 cut(s) 713
BstV1I GCAGC 4 cut(s) 422, 722, 768, 808
BstV2I GAAGAC 1 cut(s) 47
BsuRI GGCC 2 cut(s) 492, 856
BtgI CCRYGG 2 cut(s) 132, 857
BtsCI GGATG 2 cut(s) 984, 1036
BtsI GCAGTG 1 cut(s) 672
BtsIMutI CAGTG 2 cut(s) 291, 672
BveI ACCTGC 2 cut(s) 455, 643
Cac8I GCNNGC 7 cut(s) 246, 494, 498, 513, 826, 988, 1042
CaiI CAGNNNCTG 1 cut(s) 635
Cfr10I RCCGGY 2 cut(s) 98, 492
Cfr13I GGNCC 4 cut(s) 385, 428, 478, 632
CseI GACGC 1 cut(s) 579
Csp6I GTAC 1 cut(s) 536
CspCI CAANNNNNGTGG 2 cut(s) 406, 441
CviAII CATG 5 cut(s) 133, 193, 585, 787, 858
CviQI GTAC 1 cut(s) 536
DdeI CTNAG 2 cut(s) 202, 947
DpnI GATC 2 cut(s) 290, 1007
DpnII GATC 2 cut(s) 288, 1005
EaeI YGGCCR 1 cut(s) 490
Ecl136II GAGCTC 1 cut(s) 613
Eco130I CCWWGG 4 cut(s) 132, 388, 857, 909
Eco24I GRGCYC 2 cut(s) 141, 615
Eco31I GGTCTC 3 cut(s) 125, 212, 682
Eco47I GGWCC 4 cut(s) 385, 428, 478, 632
Eco53kI GAGCTC 1 cut(s) 613
Eco88I CYCGRG 2 cut(s) 597, 941
EcoICRI GAGCTC 1 cut(s) 613
EcoO109I RGGNCCY 1 cut(s) 632
EcoRII CCWGG 1 cut(s) 713
EcoT14I CCWWGG 4 cut(s) 132, 388, 857, 909
EcoT38I GRGCYC 2 cut(s) 141, 615
ErhI CCWWGG 4 cut(s) 132, 388, 857, 909
FaeI CATG 5 cut(s) 136, 196, 588, 790, 861
FaqI GGGAC 1 cut(s) 137
FatI CATG 5 cut(s) 132, 192, 584, 786, 857
FauI CCCGC 1 cut(s) 368
FblI GTMKAC 1 cut(s) 593
Fnu4HI GCNGC 4 cut(s) 436, 711, 782, 822
FokI GGATG 2 cut(s) 991, 1043
FriOI GRGCYC 2 cut(s) 141, 615
Fsp4HI GCNGC 4 cut(s) 436, 711, 782, 822
FspBI CTAG 2 cut(s) 36, 452
GluI GCNGC 4 cut(s) 436, 711, 782, 822
HaeIII GGCC 2 cut(s) 492, 856
HapII CCGG 3 cut(s) 99, 286, 493
HgaI GACGC 1 cut(s) 579
Hin1II CATG 5 cut(s) 136, 196, 588, 790, 861
HincII GTYRAC 2 cut(s) 486, 594
HindII GTYRAC 2 cut(s) 486, 594
HindIII AAGCTT 1 cut(s) 419
HinfI GANTC 8 cut(s) 43, 472, 595, 600, 888, 966, 1020, 1122
HpaI GTTAAC 1 cut(s) 486
HpaII CCGG 3 cut(s) 99, 286, 493
HphI GGTGA 3 cut(s) 88, 577, 712
Hpy166II GTNNAC 5 cut(s) 7, 428, 486, 594, 953
Hpy188I TCNGA 3 cut(s) 48, 521, 617
Hpy188III TCNNGA 4 cut(s) 112, 286, 398, 970
Hpy8I GTNNAC 5 cut(s) 7, 428, 486, 594, 953
Hpy99I CGWCG 1 cut(s) 595
HpyAV CCTTC 4 cut(s) 350, 365, 905, 937
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4V TGCA 7 cut(s) 248, 275, 320, 638, 650, 875, 1044
HpyF10VI GCNNNNNNNGC 7 cut(s) 169, 512, 587, 626, 635, 872, 1046
HpyF3I CTNAG 2 cut(s) 202, 947
Hsp92II CATG 5 cut(s) 136, 196, 588, 790, 861
Kpn2I TCCGGA 1 cut(s) 285
KroI GCCGGC 1 cut(s) 492
KroNI GCCGGC 1 cut(s) 494
KspAI GTTAAC 1 cut(s) 486
Kzo9I GATC 2 cut(s) 288, 1005
LmnI GCTCC 2 cut(s) 136, 618
Lsp1109I GCAGC 4 cut(s) 422, 722, 768, 808
LweI GCATC 4 cut(s) 186, 558, 768, 1117
MaeI CTAG 2 cut(s) 36, 452
MaeIII GTNAC 1 cut(s) 94
MalI GATC 2 cut(s) 290, 1007
MboI GATC 2 cut(s) 288, 1005
MboII GAAGA 4 cut(s) 52, 781, 784, 1046
MfeI CAATTG 1 cut(s) 1024
MhlI GDGCHC 2 cut(s) 141, 615
MluCI AATT 4 cut(s) 261, 321, 808, 1024
MlyI GAGTC 4 cut(s) 37, 589, 609, 1131
MroI TCCGGA 1 cut(s) 285
MroNI GCCGGC 1 cut(s) 492
MseI TTAA 5 cut(s) 345, 485, 936, 1016, 1089
MspI CCGG 3 cut(s) 99, 286, 493
MspR9I CCNGG 1 cut(s) 715
MunI CAATTG 1 cut(s) 1024
MvaI CCWGG 1 cut(s) 715
MwoI GCNNNNNNNGC 7 cut(s) 169, 512, 587, 626, 635, 872, 1046
NaeI GCCGGC 1 cut(s) 494
NcoI CCATGG 2 cut(s) 132, 857
NdeII GATC 2 cut(s) 288, 1005
NgoMIV GCCGGC 1 cut(s) 492
NlaIII CATG 5 cut(s) 136, 196, 588, 790, 861
NlaIV GGNNCC 3 cut(s) 130, 138, 186
NmuCI GTSAC 1 cut(s) 94
NspI RCATGY 1 cut(s) 588
PaeR7I CTCGAG 1 cut(s) 597
PciI ACATGT 1 cut(s) 584
PdiI GCCGGC 1 cut(s) 494
PfeI GAWTC 4 cut(s) 472, 888, 966, 1020
PkrI GCNGC 4 cut(s) 437, 712, 783, 823
PleI GAGTC 4 cut(s) 37, 589, 608, 1130
PpsI GAGTC 4 cut(s) 37, 589, 608, 1130
PpuMI RGGWCCY 1 cut(s) 632
PscI ACATGT 1 cut(s) 584
PshAI GACNNNNGTC 1 cut(s) 599
Psp124BI GAGCTC 1 cut(s) 615
Psp5II RGGWCCY 1 cut(s) 632
Psp6I CCWGG 1 cut(s) 713
PspGI CCWGG 1 cut(s) 713
PspN4I GGNNCC 3 cut(s) 130, 138, 186
PspPI GGNCC 4 cut(s) 385, 428, 478, 632
PspPPI RGGWCCY 1 cut(s) 632
PspXI VCTCGAGB 1 cut(s) 597
PstNI CAGNNNCTG 1 cut(s) 635
RsaI GTAC 1 cut(s) 537
RsaNI GTAC 1 cut(s) 536
SacI GAGCTC 1 cut(s) 615
SalI GTCGAC 1 cut(s) 592
SaqAI TTAA 5 cut(s) 345, 485, 936, 1016, 1089
SatI GCNGC 4 cut(s) 436, 711, 782, 822
Sau3AI GATC 2 cut(s) 288, 1005
Sau96I GGNCC 4 cut(s) 385, 428, 478, 632
SchI GAGTC 4 cut(s) 37, 589, 609, 1131
ScrFI CCNGG 1 cut(s) 715
SduI GDGCHC 2 cut(s) 141, 615
SfaNI GCATC 4 cut(s) 186, 558, 768, 1117
Sfr274I CTCGAG 1 cut(s) 597
SinI GGWCC 4 cut(s) 385, 428, 478, 632
SlaI CTCGAG 1 cut(s) 597
SmlI CTYRAG 3 cut(s) 112, 416, 597
SmoI CTYRAG 3 cut(s) 112, 416, 597
Sse9I AATT 4 cut(s) 261, 321, 808, 1024
SsiI CCGC 2 cut(s) 375, 828
SspMI CTAG 2 cut(s) 36, 452
SstI GAGCTC 1 cut(s) 615
StyD4I CCNGG 1 cut(s) 713
StyI CCWWGG 4 cut(s) 132, 388, 857, 909
TaaI ACNGT 1 cut(s) 184
TaqI TCGA 5 cut(s) 593, 598, 841, 930, 1125
TasI AATT 4 cut(s) 261, 321, 808, 1024
TfiI GAWTC 4 cut(s) 472, 888, 966, 1020
Tru1I TTAA 5 cut(s) 345, 485, 936, 1016, 1089
Tru9I TTAA 5 cut(s) 345, 485, 936, 1016, 1089
TscAI CASTG 2 cut(s) 298, 672
TseFI GTSAC 1 cut(s) 94
TseI GCWGC 4 cut(s) 435, 710, 781, 821
Tsp45I GTSAC 1 cut(s) 94
TspDTI ATGAA 1 cut(s) 1047
TspRI CASTG 2 cut(s) 298, 672
VpaK11BI GGWCC 4 cut(s) 385, 428, 478, 632
XapI RAATTY 1 cut(s) 808
XceI RCATGY 1 cut(s) 588
XhoI CTCGAG 1 cut(s) 597
XmiI GTMKAC 1 cut(s) 593
XspI CTAG 2 cut(s) 36, 452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.