Rh7DG192100

Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0007089
Physical Location & Seq
Forward (+)
17936470 .. 17939903
3434 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG192100.1

Sequence Viewer

Length: 528 bp
ATGCTGTCTCTGGCAACATGTGCGTCGACTCGAGTCTCAAATGAGCTCGGAGCAAACAGGGCAGGACCTGCATACCAATCTGCATTTGTGTCTCTGGCAGTGAGTATGGTCTCTGGTTGTTTAGGTGGATTGGTGATGGTAGCAGCCAGGGGAAGTTGGGGGTCGTTGTTTAGCCACGATAAGGGGATTGTAAGAGGTGTGAAGAAGATGATGCTGCTCATGGCTTTGGTGGAGGTTGTGAATTTCCCATTAGCTGCTTGCGGAGGGATTGTTCGAGGAACAGCGAGGCCATGGTTGGCTATGTATGCAAATCTTGGTGGATTCTACCTTCTGGCTTTGCCCTTGGGAGTTGTTTTAGCCTTCGAGGAGGGATCCCAACAGAAGCGTGCAAAGCACAAAGACTTGCCTCACTTTCCTGTCAAGCACCAGAACTTAACAAACACGAAAACCACAAAAGCGTGGGGAGAGTCGATGCCGAAGTCTAAAAGTAACCAGAAATGTCCTCCAACTCCAACCCACATCTTCTAG

Protein Analysis

175

Amino Acids

18.7

Weight (kDa)

9.98

Isoelectric Point (pI)

38.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MatE PF01554 1 - 117 1.1e-18 MatE
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 76
AccI GTMKAC 1 cut(s) 26
AciI CCGC 1 cut(s) 261
AclWI GGATC 2 cut(s) 366, 379
AcsI RAATTY 1 cut(s) 241
AfiI CCNNNNNNNGG 1 cut(s) 181
AflIII ACRYGT 1 cut(s) 17
AjnI CCWGG 1 cut(s) 146
AloI GAACNNNNNNTCC 2 cut(s) 255, 287
AluBI AGCT 2 cut(s) 46, 254
AluI AGCT 2 cut(s) 46, 254
Alw21I GWGCWC 1 cut(s) 48
Alw26I GTCTC 4 cut(s) 12, 40, 96, 115
AlwI GGATC 2 cut(s) 366, 379
AlwNI CAGNNNCTG 1 cut(s) 68
Ama87I CYCGRG 1 cut(s) 30
AoxI GGCC 1 cut(s) 287
ApeKI GCWGC 3 cut(s) 143, 214, 254
ApoI RAATTY 1 cut(s) 241
AspS9I GGNCC 1 cut(s) 65
AsuHPI GGTGA 1 cut(s) 145
AvaI CYCGRG 1 cut(s) 30
AvaII GGWCC 1 cut(s) 65
BamHI GGATCC 1 cut(s) 371
BanII GRGCYC 1 cut(s) 48
Bbv12I GWGCWC 1 cut(s) 48
BbvI GCAGC 3 cut(s) 155, 201, 241
BccI CCATC 1 cut(s) 130
BciT130I CCWGG 1 cut(s) 148
BcoDI GTCTC 4 cut(s) 12, 40, 96, 115
BfaI CTAG 1 cut(s) 526
BfuAI ACCTGC 1 cut(s) 76
BisI GCNGC 3 cut(s) 144, 215, 255
BlsI GCNGC 3 cut(s) 145, 216, 256
Bme1390I CCNGG 1 cut(s) 148
Bme18I GGWCC 1 cut(s) 65
BmeT110I CYCGRG 1 cut(s) 30
BmgT120I GGNCC 1 cut(s) 65
BmiI GGNNCC 1 cut(s) 373
BmrFI CCNGG 1 cut(s) 148
BmsI GCATC 2 cut(s) 201, 462
BoxI GACNNNNGTC 1 cut(s) 32
BsaI GGTCTC 1 cut(s) 115
BsaJI CCNNGG 3 cut(s) 147, 290, 342
BsaXI ACNNNNNCTCC 2 cut(s) 255, 285
Bsc4I CCNNNNNNNGG 1 cut(s) 181
BseBI CCWGG 1 cut(s) 148
BseDI CCNNGG 3 cut(s) 147, 290, 342
BseLI CCNNNNNNNGG 1 cut(s) 181
BseRI GAGGAG 1 cut(s) 380
BseXI GCAGC 3 cut(s) 155, 201, 241
BshFI GGCC 1 cut(s) 289
BsiHKAI GWGCWC 1 cut(s) 48
BsiHKCI CYCGRG 1 cut(s) 30
BslI CCNNNNNNNGG 1 cut(s) 181
BsmAI GTCTC 4 cut(s) 12, 40, 96, 115
BsnI GGCC 1 cut(s) 289
Bso31I GGTCTC 1 cut(s) 115
BsoBI CYCGRG 1 cut(s) 30
Bsp1286I GDGCHC 1 cut(s) 48
Bsp143I GATC 1 cut(s) 371
Bsp19I CCATGG 1 cut(s) 290
BspACI CCGC 1 cut(s) 261
BspANI GGCC 1 cut(s) 289
BspLI GGNNCC 1 cut(s) 373
BspMI ACCTGC 1 cut(s) 76
BspPI GGATC 2 cut(s) 366, 379
BspTNI GGTCTC 1 cut(s) 115
BssECI CCNNGG 3 cut(s) 147, 290, 342
BssMI GATC 1 cut(s) 371
BssT1I CCWWGG 2 cut(s) 290, 342
Bst2UI CCWGG 1 cut(s) 148
BstAPI GCANNNNNTGC 2 cut(s) 20, 68
BstC8I GCNNGC 2 cut(s) 259, 387
BstDSI CCRYGG 1 cut(s) 290
BstKTI GATC 1 cut(s) 374
BstMAI GTCTC 4 cut(s) 12, 40, 96, 115
BstMBI GATC 1 cut(s) 371
BstMWI GCNNNNNNNGC 5 cut(s) 20, 59, 68, 305, 391
BstNI CCWGG 1 cut(s) 148
BstNSI RCATGY 1 cut(s) 21
BstPAI GACNNNNGTC 1 cut(s) 32
BstSCI CCNGG 1 cut(s) 146
BstV1I GCAGC 3 cut(s) 155, 201, 241
BstX2I RGATCY 1 cut(s) 371
BstYI RGATCY 1 cut(s) 371
BsuRI GGCC 1 cut(s) 289
BtgI CCRYGG 1 cut(s) 290
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 1 cut(s) 105
BveI ACCTGC 1 cut(s) 76
Cac8I GCNNGC 2 cut(s) 259, 387
CaiI CAGNNNCTG 1 cut(s) 68
Cfr13I GGNCC 1 cut(s) 65
CseI GACGC 1 cut(s) 12
CviAII CATG 3 cut(s) 18, 220, 291
CviJI RGCY 9 cut(s) 46, 146, 174, 224, 254, 289, 299, 335, 359
CviKI_1 RGCY 9 cut(s) 46, 146, 174, 224, 254, 289, 299, 335, 359
DpnI GATC 1 cut(s) 373
DpnII GATC 1 cut(s) 371
Ecl136II GAGCTC 1 cut(s) 46
Eco130I CCWWGG 2 cut(s) 290, 342
Eco24I GRGCYC 1 cut(s) 48
Eco31I GGTCTC 1 cut(s) 115
Eco47I GGWCC 1 cut(s) 65
Eco53kI GAGCTC 1 cut(s) 46
Eco88I CYCGRG 1 cut(s) 30
EcoICRI GAGCTC 1 cut(s) 46
EcoO109I RGGNCCY 1 cut(s) 65
EcoRII CCWGG 1 cut(s) 146
EcoT14I CCWWGG 2 cut(s) 290, 342
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 2 cut(s) 290, 342
FaeI CATG 3 cut(s) 21, 223, 294
FaiI YATR 7 cut(s) 19, 73, 107, 221, 292, 302, 306
FatI CATG 3 cut(s) 17, 219, 290
FblI GTMKAC 1 cut(s) 26
Fnu4HI GCNGC 3 cut(s) 144, 215, 255
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 3 cut(s) 144, 215, 255
FspBI CTAG 1 cut(s) 526
GluI GCNGC 3 cut(s) 144, 215, 255
HaeIII GGCC 1 cut(s) 289
HgaI GACGC 1 cut(s) 12
Hin1II CATG 3 cut(s) 21, 223, 294
HincII GTYRAC 1 cut(s) 27
HindII GTYRAC 1 cut(s) 27
HinfI GANTC 4 cut(s) 28, 33, 321, 467
HphI GGTGA 1 cut(s) 145
Hpy166II GTNNAC 1 cut(s) 27
Hpy188I TCNGA 1 cut(s) 50
Hpy8I GTNNAC 1 cut(s) 27
Hpy99I CGWCG 1 cut(s) 28
HpyAV CCTTC 2 cut(s) 338, 370
HpyCH4V TGCA 4 cut(s) 71, 83, 308, 389
HpyF10VI GCNNNNNNNGC 5 cut(s) 20, 59, 68, 305, 391
Hsp92II CATG 3 cut(s) 21, 223, 294
Kzo9I GATC 1 cut(s) 371
LmnI GCTCC 1 cut(s) 50
Lsp1109I GCAGC 3 cut(s) 155, 201, 241
LweI GCATC 2 cut(s) 201, 462
MaeI CTAG 1 cut(s) 526
MaeIII GTNAC 1 cut(s) 488
MalI GATC 1 cut(s) 373
MboI GATC 1 cut(s) 371
MboII GAAGA 3 cut(s) 214, 217, 514
MflI RGATCY 1 cut(s) 371
MhlI GDGCHC 1 cut(s) 48
MluCI AATT 1 cut(s) 241
MlyI GAGTC 3 cut(s) 22, 42, 476
MnlI CCTC 9 cut(s) 188, 226, 257, 269, 279, 358, 361, 417, 513
MseI TTAA 1 cut(s) 434
MspR9I CCNGG 1 cut(s) 148
MvaI CCWGG 1 cut(s) 148
MwoI GCNNNNNNNGC 5 cut(s) 20, 59, 68, 305, 391
NcoI CCATGG 1 cut(s) 290
NdeII GATC 1 cut(s) 371
NlaIII CATG 3 cut(s) 21, 223, 294
NlaIV GGNNCC 1 cut(s) 373
NspI RCATGY 1 cut(s) 21
PaeR7I CTCGAG 1 cut(s) 30
PciI ACATGT 1 cut(s) 17
PfeI GAWTC 1 cut(s) 321
PkrI GCNGC 3 cut(s) 145, 216, 256
PleI GAGTC 3 cut(s) 22, 41, 475
PpsI GAGTC 3 cut(s) 22, 41, 475
PpuMI RGGWCCY 1 cut(s) 65
PscI ACATGT 1 cut(s) 17
PshAI GACNNNNGTC 1 cut(s) 32
Psp124BI GAGCTC 1 cut(s) 48
Psp5II RGGWCCY 1 cut(s) 65
Psp6I CCWGG 1 cut(s) 146
PspGI CCWGG 1 cut(s) 146
PspN4I GGNNCC 1 cut(s) 373
PspPI GGNCC 1 cut(s) 65
PspPPI RGGWCCY 1 cut(s) 65
PspXI VCTCGAGB 1 cut(s) 30
PstNI CAGNNNCTG 1 cut(s) 68
PsuI RGATCY 1 cut(s) 371
SacI GAGCTC 1 cut(s) 48
SalI GTCGAC 1 cut(s) 25
SaqAI TTAA 1 cut(s) 434
SatI GCNGC 3 cut(s) 144, 215, 255
Sau3AI GATC 1 cut(s) 371
Sau96I GGNCC 1 cut(s) 65
SchI GAGTC 3 cut(s) 22, 42, 476
ScrFI CCNGG 1 cut(s) 148
SduI GDGCHC 1 cut(s) 48
SetI ASST 7 cut(s) 48, 70, 127, 199, 237, 256, 330
SfaNI GCATC 2 cut(s) 201, 462
Sfr274I CTCGAG 1 cut(s) 30
SinI GGWCC 1 cut(s) 65
SlaI CTCGAG 1 cut(s) 30
SmlI CTYRAG 1 cut(s) 30
SmoI CTYRAG 1 cut(s) 30
Sse9I AATT 1 cut(s) 241
SsiI CCGC 1 cut(s) 261
SspMI CTAG 1 cut(s) 526
SstI GAGCTC 1 cut(s) 48
StyD4I CCNGG 1 cut(s) 146
StyI CCWWGG 2 cut(s) 290, 342
TaqI TCGA 5 cut(s) 26, 31, 274, 363, 470
TasI AATT 1 cut(s) 241
TfiI GAWTC 1 cut(s) 321
Tru1I TTAA 1 cut(s) 434
Tru9I TTAA 1 cut(s) 434
TscAI CASTG 1 cut(s) 105
TseI GCWGC 3 cut(s) 143, 214, 254
TspRI CASTG 1 cut(s) 105
VpaK11BI GGWCC 1 cut(s) 65
XapI RAATTY 1 cut(s) 241
XceI RCATGY 1 cut(s) 21
XhoI CTCGAG 1 cut(s) 30
XmiI GTMKAC 1 cut(s) 26
XspI CTAG 1 cut(s) 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.