RchiOBHm_Chr7g0222101

11S globulin seed storage protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0002867
Physical Location & Seq
Forward (+)
43206313 .. 43209820
3508 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19875

Sequence Viewer

Length: 888 bp
ATGGCAGCTGAGATGGATCTAACACCCAAGTCAGCGGCAGCGGCGTTCGAGGGAGATGGTGGAGGATATAACATATGGTCATTTCCGGCACTTGGAGAGGCCAACGTTGGCGCCGGCAAGCTTGTATTGAAGCCTAGTGGTTTTGCTCTTCCTCACTCTGCAGATTCTGCCAAACTGGGATATGTTCTTCAAGCTAAAGAAAGGAGACGTGATTCCGATACCACTCGGAGCAGTCTCATGGTGGTTCAACAATGGCGGCTCATCCAACGACCTTATTATTGTTTTCTTGGGAGAACTACTAAGGCTTACACTCCTGGTCTGTTTACTTACTTCTTCATAGCAGGAACTCAAAGTATGCTCAGAGGTTTCTCGACTGACTTCATTAGCAAGTCATTCAATATTACCAAAGATGAAGCTGATGAGGTCACCAAAAACCAGACAGGGGTTTTGCTAGTTAAGCTAGAACAGGGAAAGACCATGCCTAAGCCCAACGCACTCGTCACCCACAAACTTGTTCATCAACTCACTATTAGTGCCACTTTGACTGAGAAGGAGTTTCCTTTTCTTAACCAAGCTGGGTTAAGTGCCAACCTCATAAAACTTGAACCCTATGCAATTTCCTCTCCCATTTACACTACTGATTCTTCGGTTCAAGTGATCTATGTCGTTGGAGGAGGTGGTCGGGTCCAAATTGCGGGTCTTAATGGTCAGAGTGCGTTGGATGCAGAAGTAACTGCGGGTCACTTGATCGTTGTGCCTAGGTTTTTCATGGTAGCGAAACTTGCTGGGGAAAAAAGGAATGGAGTGTTTCTCTGTCATTACAAGTTCCCGGGCTGTCCTGGAAGACTTAACTGGCAAGACATCAGTATTGAGGGCATTATCACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

295

Amino Acids

32.09

Weight (kDa)

9.12

Isoelectric Point (pI)

37.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 179 - 270 1.8e-13 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 110
AciI CCGC 5 cut(s) 35, 41, 256, 695, 737
AclI AACGTT 1 cut(s) 105
AclWI GGATC 1 cut(s) 24
AcyI GRCGYC 1 cut(s) 111
AfiI CCNNNNNNNGG 3 cut(s) 92, 442, 694
AgsI TTSAA 6 cut(s) 130, 191, 248, 397, 605, 653
AjiI CACGTC 1 cut(s) 209
AjnI CCWGG 2 cut(s) 313, 838
AluBI AGCT 6 cut(s) 8, 121, 194, 416, 460, 575
AluI AGCT 6 cut(s) 8, 121, 194, 416, 460, 575
Alw26I GTCTC 2 cut(s) 199, 239
AlwI GGATC 1 cut(s) 24
AlwNI CAGNNNCTG 1 cut(s) 167
Ama87I CYCGRG 1 cut(s) 829
AoxI GGCC 1 cut(s) 99
ApeKI GCWGC 2 cut(s) 5, 38
ArsI GACNNNNNNTTYG 4 cut(s) 430, 462, 682, 714
AspA2I CCTAGG 1 cut(s) 758
AspLEI GCGC 1 cut(s) 113
AspS9I GGNCC 1 cut(s) 685
AsuC2I CCSGG 2 cut(s) 830, 831
AsuHPI GGTGA 3 cut(s) 418, 493, 874
AvaI CYCGRG 1 cut(s) 829
AvaII GGWCC 1 cut(s) 685
AvrII CCTAGG 1 cut(s) 758
BanI GGYRCC 1 cut(s) 110
BbsI GAAGAC 1 cut(s) 850
BbvI GCAGC 2 cut(s) 17, 50
BccI CCATC 2 cut(s) 7, 50
BciT130I CCWGG 2 cut(s) 315, 840
BcnI CCSGG 2 cut(s) 830, 831
BcoDI GTCTC 2 cut(s) 199, 239
BfaI CTAG 4 cut(s) 135, 452, 461, 759
BfmI CTRYAG 1 cut(s) 159
BfoI RGCGCY 1 cut(s) 114
BisI GCNGC 5 cut(s) 6, 36, 39, 42, 257
BlnI CCTAGG 1 cut(s) 758
BlsI GCNGC 5 cut(s) 7, 37, 40, 43, 258
Bme1390I CCNGG 4 cut(s) 315, 830, 831, 840
Bme18I GGWCC 1 cut(s) 685
BmeT110I CYCGRG 1 cut(s) 829
BmgBI CACGTC 1 cut(s) 209
BmgT120I GGNCC 1 cut(s) 685
BmiI GGNNCC 2 cut(s) 112, 686
BmrFI CCNGG 4 cut(s) 315, 830, 831, 840
BmrI ACTGGG 1 cut(s) 185
BmsI GCATC 1 cut(s) 712
BmuI ACTGGG 1 cut(s) 185
BpiI GAAGAC 1 cut(s) 850
BplI GAGNNNNNCTC 2 cut(s) 795, 827
Bpu10I CCTNAGC 1 cut(s) 483
BpuMI CCSGG 2 cut(s) 830, 831
BsaHI GRCGYC 1 cut(s) 111
BsaJI CCNNGG 2 cut(s) 758, 829
Bsc4I CCNNNNNNNGG 3 cut(s) 92, 442, 694
Bse118I RCCGGY 1 cut(s) 113
Bse1I ACTGG 2 cut(s) 180, 857
BseBI CCWGG 2 cut(s) 315, 840
BseDI CCNNGG 2 cut(s) 758, 829
BseGI GGATG 2 cut(s) 261, 727
BseLI CCNNNNNNNGG 3 cut(s) 92, 442, 694
BseMII CTCAG 2 cut(s) 373, 537
BseNI ACTGG 2 cut(s) 180, 857
BseRI GAGGAG 1 cut(s) 687
BseXI GCAGC 2 cut(s) 17, 50
BseYI CCCAGC 2 cut(s) 575, 785
BshFI GGCC 1 cut(s) 101
BshNI GGYRCC 1 cut(s) 110
BsiHKCI CYCGRG 1 cut(s) 829
BsiSI CCGG 3 cut(s) 86, 114, 830
BslI CCNNNNNNNGG 3 cut(s) 92, 442, 694
BsmAI GTCTC 2 cut(s) 199, 239
BsmBI CGTCTC 1 cut(s) 199
BsnI GGCC 1 cut(s) 101
BsoBI CYCGRG 1 cut(s) 829
Bsp143I GATC 3 cut(s) 16, 657, 747
BspACI CCGC 5 cut(s) 35, 41, 256, 695, 737
BspANI GGCC 1 cut(s) 101
BspCNI CTCAG 2 cut(s) 372, 538
BspLI GGNNCC 2 cut(s) 112, 686
BspMAI CTGCAG 1 cut(s) 163
BspPI GGATC 1 cut(s) 24
BspQI GCTCTTC 1 cut(s) 153
BspT107I GGYRCC 1 cut(s) 110
BsrFI RCCGGY 1 cut(s) 113
BsrI ACTGG 2 cut(s) 180, 857
BssAI RCCGGY 1 cut(s) 113
BssECI CCNNGG 2 cut(s) 758, 829
BssMI GATC 3 cut(s) 16, 657, 747
BssNI GRCGYC 1 cut(s) 111
BssT1I CCWWGG 1 cut(s) 758
Bst2UI CCWGG 2 cut(s) 315, 840
Bst6I CTCTTC 1 cut(s) 153
BstACI GRCGYC 1 cut(s) 111
BstAPI GCANNNNNTGC 1 cut(s) 167
BstC8I GCNNGC 2 cut(s) 115, 119
BstDEI CTNAG 5 cut(s) 9, 300, 359, 483, 546
BstEII GGTNACC 1 cut(s) 424
BstF5I GGATG 2 cut(s) 261, 727
BstH2I RGCGCY 1 cut(s) 114
BstHHI GCGC 1 cut(s) 113
BstKTI GATC 3 cut(s) 19, 660, 750
BstMAI GTCTC 2 cut(s) 199, 239
BstMBI GATC 3 cut(s) 16, 657, 747
BstMWI GCNNNNNNNGC 5 cut(s) 41, 167, 457, 722, 782
BstNI CCWGG 2 cut(s) 315, 840
BstPI GGTNACC 1 cut(s) 424
BstSCI CCNGG 4 cut(s) 313, 828, 829, 838
BstSFI CTRYAG 1 cut(s) 159
BstV1I GCAGC 2 cut(s) 17, 50
BstV2I GAAGAC 1 cut(s) 850
BstX2I RGATCY 1 cut(s) 16
BstYI RGATCY 1 cut(s) 16
BsuRI GGCC 1 cut(s) 101
BtrI CACGTC 1 cut(s) 209
BtsCI GGATG 2 cut(s) 261, 727
Cac8I GCNNGC 2 cut(s) 115, 119
CaiI CAGNNNCTG 1 cut(s) 167
CfoI GCGC 1 cut(s) 113
Cfr10I RCCGGY 1 cut(s) 113
Cfr13I GGNCC 1 cut(s) 685
Cfr9I CCCGGG 1 cut(s) 829
CviAII CATG 3 cut(s) 238, 478, 769
DdeI CTNAG 5 cut(s) 9, 300, 359, 483, 546
DinI GGCGCC 1 cut(s) 112
DpnI GATC 3 cut(s) 18, 659, 749
DpnII GATC 3 cut(s) 16, 657, 747
Eam1104I CTCTTC 1 cut(s) 153
EarI CTCTTC 1 cut(s) 153
Eco130I CCWWGG 1 cut(s) 758
Eco47I GGWCC 1 cut(s) 685
Eco88I CYCGRG 1 cut(s) 829
Eco91I GGTNACC 1 cut(s) 424
EcoO65I GGTNACC 1 cut(s) 424
EcoRII CCWGG 2 cut(s) 313, 838
EcoT14I CCWWGG 1 cut(s) 758
EgeI GGCGCC 1 cut(s) 112
EheI GGCGCC 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 758
Esp3I CGTCTC 1 cut(s) 199
FaeI CATG 3 cut(s) 241, 481, 772
FatI CATG 3 cut(s) 237, 477, 768
FauI CCCGC 2 cut(s) 688, 730
FauNDI CATATG 1 cut(s) 74
Fnu4HI GCNGC 5 cut(s) 6, 36, 39, 42, 257
FokI GGATG 2 cut(s) 248, 734
Fsp4HI GCNGC 5 cut(s) 6, 36, 39, 42, 257
FspBI CTAG 4 cut(s) 135, 452, 461, 759
GlaI GCGC 1 cut(s) 112
GluI GCNGC 5 cut(s) 6, 36, 39, 42, 257
GsaI CCCAGC 2 cut(s) 579, 789
HaeII RGCGCY 1 cut(s) 114
HaeIII GGCC 1 cut(s) 101
HapII CCGG 3 cut(s) 86, 114, 830
HhaI GCGC 1 cut(s) 113
Hin1I GRCGYC 1 cut(s) 111
Hin1II CATG 3 cut(s) 241, 481, 772
Hin6I GCGC 1 cut(s) 111
HinP1I GCGC 1 cut(s) 111
HindIII AAGCTT 1 cut(s) 119
HinfI GANTC 3 cut(s) 164, 212, 641
HpaII CCGG 3 cut(s) 86, 114, 830
HphI GGTGA 3 cut(s) 418, 493, 874
Hpy166II GTNNAC 1 cut(s) 324
Hpy188I TCNGA 4 cut(s) 217, 228, 362, 711
Hpy188III TCNNGA 1 cut(s) 370
Hpy8I GTNNAC 1 cut(s) 324
HpyAV CCTTC 1 cut(s) 544
HpyCH4IV ACGT 2 cut(s) 105, 208
HpyCH4V TGCA 3 cut(s) 161, 614, 725
HpyF10VI GCNNNNNNNGC 5 cut(s) 41, 167, 457, 722, 782
HpyF3I CTNAG 5 cut(s) 9, 300, 359, 483, 546
HpySE526I ACGT 2 cut(s) 105, 208
Hsp92I GRCGYC 1 cut(s) 111
Hsp92II CATG 3 cut(s) 241, 481, 772
HspAI GCGC 1 cut(s) 111
KasI GGCGCC 1 cut(s) 110
KroI GCCGGC 1 cut(s) 113
KroNI GCCGGC 1 cut(s) 115
Kzo9I GATC 3 cut(s) 16, 657, 747
LguI GCTCTTC 1 cut(s) 153
LmnI GCTCC 1 cut(s) 228
Lsp1109I GCAGC 2 cut(s) 17, 50
LweI GCATC 1 cut(s) 712
MaeI CTAG 4 cut(s) 135, 452, 461, 759
MaeII ACGT 2 cut(s) 105, 208
MaeIII GTNAC 4 cut(s) 424, 499, 730, 740
MalI GATC 3 cut(s) 18, 659, 749
MboI GATC 3 cut(s) 16, 657, 747
MboII GAAGA 5 cut(s) 140, 179, 325, 636, 855
MflI RGATCY 1 cut(s) 16
MluCI AATT 2 cut(s) 615, 690
Mly113I GGCGCC 1 cut(s) 111
MmeI TCCRAC 3 cut(s) 289, 649, 699
MroNI GCCGGC 1 cut(s) 113
MseI TTAA 5 cut(s) 456, 567, 581, 702, 849
MspA1I CMGCKG 3 cut(s) 8, 35, 41
MspI CCGG 3 cut(s) 86, 114, 830
MspR9I CCNGG 4 cut(s) 315, 830, 831, 840
MvaI CCWGG 2 cut(s) 315, 840
MwoI GCNNNNNNNGC 5 cut(s) 41, 167, 457, 722, 782
NaeI GCCGGC 1 cut(s) 115
NarI GGCGCC 1 cut(s) 111
NciI CCSGG 2 cut(s) 830, 831
NdeI CATATG 1 cut(s) 74
NdeII GATC 3 cut(s) 16, 657, 747
NgoMIV GCCGGC 1 cut(s) 113
NlaIII CATG 3 cut(s) 241, 481, 772
NlaIV GGNNCC 2 cut(s) 112, 686
NmuCI GTSAC 3 cut(s) 424, 499, 740
PciSI GCTCTTC 1 cut(s) 153
PdiI GCCGGC 1 cut(s) 115
PfeI GAWTC 3 cut(s) 164, 212, 641
PfoI TCCNGGA 1 cut(s) 838
PkrI GCNGC 5 cut(s) 7, 37, 40, 43, 258
PluTI GGCGCC 1 cut(s) 114
Psp1406I AACGTT 1 cut(s) 105
Psp6I CCWGG 2 cut(s) 313, 838
PspEI GGTNACC 1 cut(s) 424
PspFI CCCAGC 2 cut(s) 575, 785
PspGI CCWGG 2 cut(s) 313, 838
PspN4I GGNNCC 2 cut(s) 112, 686
PspPI GGNCC 1 cut(s) 685
PstI CTGCAG 1 cut(s) 163
PstNI CAGNNNCTG 1 cut(s) 167
PsuI RGATCY 1 cut(s) 16
PvuII CAGCTG 1 cut(s) 8
SapI GCTCTTC 1 cut(s) 153
SaqAI TTAA 5 cut(s) 456, 567, 581, 702, 849
SatI GCNGC 5 cut(s) 6, 36, 39, 42, 257
Sau3AI GATC 3 cut(s) 16, 657, 747
Sau96I GGNCC 1 cut(s) 685
ScrFI CCNGG 4 cut(s) 315, 830, 831, 840
SfaNI GCATC 1 cut(s) 712
SfcI CTRYAG 1 cut(s) 159
SfoI GGCGCC 1 cut(s) 112
SinI GGWCC 1 cut(s) 685
SmaI CCCGGG 1 cut(s) 831
Sse9I AATT 2 cut(s) 615, 690
SsiI CCGC 5 cut(s) 35, 41, 256, 695, 737
SspDI GGCGCC 1 cut(s) 110
SspI AATATT 1 cut(s) 400
SspMI CTAG 4 cut(s) 135, 452, 461, 759
StyD4I CCNGG 4 cut(s) 313, 828, 829, 838
StyI CCWWGG 1 cut(s) 758
TaiI ACGT 2 cut(s) 108, 211
TaqI TCGA 2 cut(s) 48, 371
TasI AATT 2 cut(s) 615, 690
TauI GCSGC 3 cut(s) 38, 44, 259
TfiI GAWTC 3 cut(s) 164, 212, 641
Tru1I TTAA 5 cut(s) 456, 567, 581, 702, 849
Tru9I TTAA 5 cut(s) 456, 567, 581, 702, 849
TseFI GTSAC 3 cut(s) 424, 499, 740
TseI GCWGC 2 cut(s) 5, 38
Tsp45I GTSAC 3 cut(s) 424, 499, 740
TspDTI ATGAA 5 cut(s) 325, 370, 426, 506, 757
TspMI CCCGGG 1 cut(s) 829
VpaK11BI GGWCC 1 cut(s) 685
XmaI CCCGGG 1 cut(s) 829
XmaJI CCTAGG 1 cut(s) 758
XspI CTAG 4 cut(s) 135, 452, 461, 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.