RchiOBHm_Chr7g0222361

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0009761
Physical Location & Seq
Forward (+)
43721277 .. 43723154
1878 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19901

Sequence Viewer

Length: 1428 bp
ATGTTTTCTATAACAAGCATGCCTTCAACAACATCAGTTCTATCAACCTACACCACATTTTCTGCTGCAGCAATGCTGATCAGGACAGTGATCAGTGAGGTCCAGACTATGGTCGGCCAGTTCATACCTCAAAAACTCCAGGACAAAATCATTGCCACACTTGCAGGCCTATTCGGTAACCTTCCTAATTCTCAAATGACTCTTATCATTGATGAAAACAATGGCCTCTCTATCAATGAAGTTTACCAGGCTTCAGAACTCTACTTAAGCACAAGGATCACTGCCTCAATTGATCAACTTAAGGTTTCCAAGTCCCCACGCCAAAACAACTTCTCTGTTACCATAAACAAAGGCGAGAAGATCACTGACGAGTTTGAAGGAATACAGTTGAGGTGGGAATTCTGTTGTTCTGAAACGAAACAGGCGAATATGGACTATGAAAGCTATTGCTCATCAACAGAAGTAGTAGAGCATAAGTCAATTCAGCTCAGTTTCCACAAGCAATACAGAGAAAAGGTATTAGAAACTTACCTGCCATATGTAGTGCAAAGATCAAAAGCAATTAAAGAGGAAAATAAGGTGGTGAGGCTGCATGCACTTGGAAACTTTGGTGGAGAATGTTCGATCGGTCCCTGGGGTTCAGTCATCTTTGAACATCCATCAACTTTTGACACATTGGCAATGGACCCAATGCTGAAGAAAGAGTTGATTGCTGACTTGGACAGGTTTGTGAAGCGAAAGGAATTTTATGGCAGAGTAGGCAAGGCATGGAAACGAGGGTACTTGTTGTATGGTCCTCCTGGCACTGGAAAGTCAAGCTTGATTGCAGCCATGGCTAATTACCTCAAGTTTGATATCTATGATTTGGAGCTTACATATTTACACAGCAACTCTGAGCTTAGGAGACTGTTAGTCTCCACCGCAAATCGATCAATCATAGTGATTGAGGATATTGACTGCAGCATTGAATTGAAGGACAGACAAGTTAGAGGAACCAACCCCAGTGACAGCCAGTTGACACTATCCGGATTGCTCAATTTCATTGATGGTTTATGGTCAAGCTGTGGAGATGAGAGGATAATTGTGTTTACCACCAACTACAAGGACAAGCTAGATCCTGCATTGGTGAGACCAGGCCGAATGGATATGCATGTTCACATGTCCTACTGCACACCGTGTGGATTCAAGACCCTTGCTCATAACTACCTTAAAGTAAAAACTCATCATCTCTTCAATGAAATTGAAACATTCATAAAGGAGGTTGATACGACTCCAGCAGAGATTGCAGAAGAACTCATGAAAAGTGAAGACACCGATGTAGCGCTGGAAGGACTCATTGCATTCCTGAGGAAGAAGAAAGAGATGAACTGCAATGCTGAAGCCAATGTTGAAGAAACAATAGAAAATAATGAACTAAAGGAAGAATGA

Protein Analysis

475

Amino Acids

53.85

Weight (kDa)

5.56

Isoelectric Point (pI)

35.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 42 - 136 3.2e-24 Domain associated at C-terminal with AAA
AAA PF00004 261 - 388 2.3e-20 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 390 - 459 1.4e-24 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 540
AccB7I CCANNNNNTGG 1 cut(s) 109
AccIII TCCGGA 1 cut(s) 1025
AciI CCGC 1 cut(s) 921
AclWI GGATC 2 cut(s) 284, 1109
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 2 cut(s) 398, 743
AcuI CTGAAG 3 cut(s) 237, 716, 1398
AdeI CACNNNGTG 2 cut(s) 1176, 1178
AfaI GTAC 1 cut(s) 782
AfeI AGCGCT 1 cut(s) 1323
AfiI CCNNNNNNNGG 2 cut(s) 109, 806
AflII CTTAAG 2 cut(s) 265, 299
AflIII ACRYGT 1 cut(s) 1158
AgsI TTSAA 9 cut(s) 27, 377, 653, 968, 973, 1186, 1234, 1244, 1391
AhdI GACNNNNNGTC 1 cut(s) 911
AjnI CCWGG 5 cut(s) 138, 246, 632, 799, 1132
AluBI AGCT 7 cut(s) 444, 487, 819, 871, 898, 1062, 1111
AluI AGCT 7 cut(s) 444, 487, 819, 871, 898, 1062, 1111
Alw26I GTCTC 3 cut(s) 898, 919, 1123
AlwI GGATC 2 cut(s) 284, 1109
Aor13HI TCCGGA 1 cut(s) 1025
Aor51HI AGCGCT 1 cut(s) 1323
AoxI GGCC 4 cut(s) 115, 166, 223, 1135
ApeKI GCWGC 5 cut(s) 65, 68, 589, 827, 960
ApoI RAATTY 2 cut(s) 398, 743
AspLEI GCGC 1 cut(s) 1324
AspS9I GGNCC 4 cut(s) 100, 629, 685, 794
AsuHPI GGTGA 2 cut(s) 595, 1138
AvaII GGWCC 4 cut(s) 100, 629, 685, 794
AxyI CCTNAGG 1 cut(s) 1346
BbsI GAAGAC 1 cut(s) 1314
BbvI GCAGC 5 cut(s) 52, 80, 576, 839, 972
BccI CCATC 2 cut(s) 667, 1040
BciT130I CCWGG 5 cut(s) 140, 248, 634, 801, 1134
BclI TGATCA 3 cut(s) 78, 90, 292
BcoDI GTCTC 3 cut(s) 898, 919, 1123
BfaI CTAG 1 cut(s) 1112
BfmI CTRYAG 2 cut(s) 66, 958
BfoI RGCGCY 1 cut(s) 1325
BfrI CTTAAG 2 cut(s) 265, 299
BfuAI ACCTGC 1 cut(s) 540
BisI GCNGC 5 cut(s) 66, 69, 590, 828, 961
BlsI GCNGC 5 cut(s) 67, 70, 591, 829, 962
Bme1390I CCNGG 5 cut(s) 140, 248, 634, 801, 1134
Bme18I GGWCC 4 cut(s) 100, 629, 685, 794
BmeRI GACNNNNNGTC 1 cut(s) 911
BmgT120I GGNCC 4 cut(s) 100, 629, 685, 794
BmiI GGNNCC 3 cut(s) 631, 687, 994
BmrFI CCNGG 5 cut(s) 140, 248, 634, 801, 1134
BmrI ACTGGG 1 cut(s) 996
BmuI ACTGGG 1 cut(s) 996
BoxI GACNNNNGTC 1 cut(s) 110
BpiI GAAGAC 1 cut(s) 1314
BpmI CTGGAG 2 cut(s) 122, 1257
Bpu10I CCTNAGC 1 cut(s) 899
BpuEI CTTGAG 1 cut(s) 830
Bsa29I ATCGAT 1 cut(s) 928
BsaI GGTCTC 1 cut(s) 1123
BsaJI CCNNGG 3 cut(s) 632, 633, 831
BsaWI WCCGGW 1 cut(s) 1025
Bsc4I CCNNNNNNNGG 2 cut(s) 109, 806
Bse1I ACTGG 4 cut(s) 118, 811, 1002, 1012
Bse21I CCTNAGG 1 cut(s) 1346
Bse3DI GCAATG 5 cut(s) 78, 150, 687, 1335, 1378
BseAI TCCGGA 1 cut(s) 1025
BseBI CCWGG 5 cut(s) 140, 248, 634, 801, 1134
BseCI ATCGAT 1 cut(s) 928
BseDI CCNNGG 3 cut(s) 632, 633, 831
BseGI GGATG 1 cut(s) 655
BseLI CCNNNNNNNGG 2 cut(s) 109, 806
BseMI GCAATG 5 cut(s) 78, 150, 687, 1335, 1378
BseMII CTCAG 3 cut(s) 502, 885, 1337
BseNI ACTGG 4 cut(s) 118, 811, 1002, 1012
BseXI GCAGC 5 cut(s) 52, 80, 576, 839, 972
BsgI GTGCAG 1 cut(s) 1153
Bsh1285I CGRYCG 1 cut(s) 627
BshFI GGCC 4 cut(s) 117, 168, 225, 1137
BshVI ATCGAT 1 cut(s) 928
BsiEI CGRYCG 1 cut(s) 627
BsiSI CCGG 1 cut(s) 1026
BslFI GGGAC 2 cut(s) 298, 615
BslI CCNNNNNNNGG 2 cut(s) 109, 806
BsmAI GTCTC 3 cut(s) 898, 919, 1123
BsmFI GGGAC 2 cut(s) 298, 615
BsmI GAATGC 1 cut(s) 1340
BsnI GGCC 4 cut(s) 117, 168, 225, 1137
Bso31I GGTCTC 1 cut(s) 1123
Bsp13I TCCGGA 1 cut(s) 1025
Bsp143I GATC 9 cut(s) 78, 90, 276, 292, 360, 551, 624, 929, 1114
Bsp19I CCATGG 1 cut(s) 831
BspACI CCGC 1 cut(s) 921
BspANI GGCC 4 cut(s) 117, 168, 225, 1137
BspCNI CTCAG 3 cut(s) 501, 886, 1338
BspDI ATCGAT 1 cut(s) 928
BspEI TCCGGA 1 cut(s) 1025
BspHI TCATGA 1 cut(s) 1296
BspLI GGNNCC 3 cut(s) 631, 687, 994
BspMAI CTGCAG 2 cut(s) 70, 962
BspMI ACCTGC 1 cut(s) 540
BspPI GGATC 2 cut(s) 284, 1109
BspTI CTTAAG 2 cut(s) 265, 299
BspTNI GGTCTC 1 cut(s) 1123
BsrDI GCAATG 5 cut(s) 78, 150, 687, 1335, 1378
BsrI ACTGG 4 cut(s) 118, 811, 1002, 1012
BssECI CCNNGG 3 cut(s) 632, 633, 831
BssMI GATC 9 cut(s) 78, 90, 276, 292, 360, 551, 624, 929, 1114
BssT1I CCWWGG 1 cut(s) 831
Bst2UI CCWGG 5 cut(s) 140, 248, 634, 801, 1134
Bst4CI ACNGT 4 cut(s) 88, 387, 909, 1176
Bst6I CTCTTC 1 cut(s) 1235
BstAFI CTTAAG 2 cut(s) 265, 299
BstAPI GCANNNNNTGC 1 cut(s) 1283
BstC8I GCNNGC 3 cut(s) 20, 166, 594
BstDEI CTNAG 4 cut(s) 488, 894, 899, 1346
BstDSI CCRYGG 1 cut(s) 831
BstEII GGTNACC 1 cut(s) 176
BstF5I GGATG 1 cut(s) 655
BstH2I RGCGCY 1 cut(s) 1325
BstHHI GCGC 1 cut(s) 1324
BstKTI GATC 9 cut(s) 81, 93, 279, 295, 363, 554, 627, 932, 1117
BstMAI GTCTC 3 cut(s) 898, 919, 1123
BstMBI GATC 9 cut(s) 78, 90, 276, 292, 360, 551, 624, 929, 1114
BstMCI CGRYCG 1 cut(s) 627
BstMWI GCNNNNNNNGC 4 cut(s) 161, 759, 833, 1283
BstNI CCWGG 5 cut(s) 140, 248, 634, 801, 1134
BstNSI RCATGY 4 cut(s) 22, 596, 1154, 1162
BstPAI GACNNNNGTC 1 cut(s) 110
BstPI GGTNACC 1 cut(s) 176
BstSCI CCNGG 5 cut(s) 138, 246, 632, 799, 1132
BstSFI CTRYAG 2 cut(s) 66, 958
BstV1I GCAGC 5 cut(s) 52, 80, 576, 839, 972
BstV2I GAAGAC 1 cut(s) 1314
BstX2I RGATCY 1 cut(s) 1114
BstYI RGATCY 1 cut(s) 1114
Bsu15I ATCGAT 1 cut(s) 928
Bsu36I CCTNAGG 1 cut(s) 1346
BsuRI GGCC 4 cut(s) 117, 168, 225, 1137
BsuTUI ATCGAT 1 cut(s) 928
BtgI CCRYGG 1 cut(s) 831
BtsCI GGATG 1 cut(s) 655
BtsI GCAGTG 1 cut(s) 279
BtsIMutI CAGTG 6 cut(s) 93, 100, 279, 363, 804, 1009
BveI ACCTGC 1 cut(s) 540
Cac8I GCNNGC 3 cut(s) 20, 166, 594
CciI TCATGA 1 cut(s) 1296
CfoI GCGC 1 cut(s) 1324
Cfr13I GGNCC 4 cut(s) 100, 629, 685, 794
ClaI ATCGAT 1 cut(s) 928
Csp6I GTAC 1 cut(s) 781
CviAII CATG 7 cut(s) 19, 593, 768, 832, 1151, 1159, 1297
CviQI GTAC 1 cut(s) 781
DdeI CTNAG 4 cut(s) 488, 894, 899, 1346
DpnI GATC 9 cut(s) 80, 92, 278, 294, 362, 553, 626, 931, 1116
DpnII GATC 9 cut(s) 78, 90, 276, 292, 360, 551, 624, 929, 1114
DraIII CACNNNGTG 2 cut(s) 1176, 1178
DriI GACNNNNNGTC 1 cut(s) 911
EaeI YGGCCR 1 cut(s) 115
Eam1104I CTCTTC 1 cut(s) 1235
Eam1105I GACNNNNNGTC 1 cut(s) 911
EarI CTCTTC 1 cut(s) 1235
Eco130I CCWWGG 1 cut(s) 831
Eco147I AGGCCT 1 cut(s) 168
Eco31I GGTCTC 1 cut(s) 1123
Eco32I GATATC 1 cut(s) 856
Eco47I GGWCC 4 cut(s) 100, 629, 685, 794
Eco47III AGCGCT 1 cut(s) 1323
Eco57I CTGAAG 3 cut(s) 237, 716, 1398
Eco81I CCTNAGG 1 cut(s) 1346
Eco91I GGTNACC 1 cut(s) 176
EcoO65I GGTNACC 1 cut(s) 176
EcoRI GAATTC 1 cut(s) 398
EcoRII CCWGG 5 cut(s) 138, 246, 632, 799, 1132
EcoRV GATATC 1 cut(s) 856
EcoT14I CCWWGG 1 cut(s) 831
EcoT22I ATGCAT 1 cut(s) 1152
ErhI CCWWGG 1 cut(s) 831
FaeI CATG 7 cut(s) 22, 596, 771, 835, 1154, 1162, 1300
FalI AAGNNNNNCTT 4 cut(s) 7, 39, 803, 835
FaqI GGGAC 2 cut(s) 298, 615
FatI CATG 7 cut(s) 18, 592, 767, 831, 1150, 1158, 1296
FauNDI CATATG 1 cut(s) 538
FbaI TGATCA 3 cut(s) 78, 90, 292
Fnu4HI GCNGC 5 cut(s) 66, 69, 590, 828, 961
FokI GGATG 1 cut(s) 642
Fsp4HI GCNGC 5 cut(s) 66, 69, 590, 828, 961
FspBI CTAG 1 cut(s) 1112
GlaI GCGC 1 cut(s) 1323
GluI GCNGC 5 cut(s) 66, 69, 590, 828, 961
GsuI CTGGAG 2 cut(s) 122, 1257
HaeII RGCGCY 1 cut(s) 1325
HaeIII GGCC 4 cut(s) 117, 168, 225, 1137
HapII CCGG 1 cut(s) 1026
HhaI GCGC 1 cut(s) 1324
Hin1II CATG 7 cut(s) 22, 596, 771, 835, 1154, 1162, 1300
Hin6I GCGC 1 cut(s) 1322
HinP1I GCGC 1 cut(s) 1322
HincII GTYRAC 1 cut(s) 1017
HindII GTYRAC 1 cut(s) 1017
HindIII AAGCTT 1 cut(s) 817
HinfI GANTC 4 cut(s) 199, 1182, 1270, 1332
HpaII CCGG 1 cut(s) 1026
HphI GGTGA 2 cut(s) 595, 1138
Hpy166II GTNNAC 4 cut(s) 244, 1017, 1089, 1156
Hpy188I TCNGA 3 cut(s) 256, 412, 895
Hpy188III TCNNGA 6 cut(s) 82, 103, 1026, 1186, 1297, 1345
Hpy8I GTNNAC 4 cut(s) 244, 1017, 1089, 1156
HpyAV CCTTC 5 cut(s) 33, 191, 371, 967, 1322
HpyCH4III ACNGT 4 cut(s) 88, 387, 909, 1176
HpyF10VI GCNNNNNNNGC 4 cut(s) 161, 759, 833, 1283
HpyF3I CTNAG 4 cut(s) 488, 894, 899, 1346
Hsp92II CATG 7 cut(s) 22, 596, 771, 835, 1154, 1162, 1300
HspAI GCGC 1 cut(s) 1322
Kpn2I TCCGGA 1 cut(s) 1025
Ksp22I TGATCA 3 cut(s) 78, 90, 292
Kzo9I GATC 9 cut(s) 78, 90, 276, 292, 360, 551, 624, 929, 1114
LmnI GCTCC 1 cut(s) 868
Lsp1109I GCAGC 5 cut(s) 52, 80, 576, 839, 972
MaeI CTAG 1 cut(s) 1112
MaeIII GTNAC 3 cut(s) 176, 337, 1004
MalI GATC 9 cut(s) 80, 92, 278, 294, 362, 553, 626, 931, 1116
MboI GATC 9 cut(s) 78, 90, 276, 292, 360, 551, 624, 929, 1114
MboII GAAGA 8 cut(s) 370, 709, 1222, 1301, 1319, 1363, 1366, 1403
MfeI CAATTG 1 cut(s) 288
MflI RGATCY 1 cut(s) 1114
MlyI GAGTC 3 cut(s) 193, 1264, 1326
Mph1103I ATGCAT 1 cut(s) 1152
MroI TCCGGA 1 cut(s) 1025
MseI TTAA 4 cut(s) 266, 300, 564, 1209
MspCI CTTAAG 2 cut(s) 265, 299
MspI CCGG 1 cut(s) 1026
MspR9I CCNGG 5 cut(s) 140, 248, 634, 801, 1134
MunI CAATTG 1 cut(s) 288
Mva1269I GAATGC 1 cut(s) 1340
MvaI CCWGG 5 cut(s) 140, 248, 634, 801, 1134
MwoI GCNNNNNNNGC 4 cut(s) 161, 759, 833, 1283
NcoI CCATGG 1 cut(s) 831
NdeI CATATG 1 cut(s) 538
NdeII GATC 9 cut(s) 78, 90, 276, 292, 360, 551, 624, 929, 1114
NlaIII CATG 7 cut(s) 22, 596, 771, 835, 1154, 1162, 1300
NlaIV GGNNCC 3 cut(s) 631, 687, 994
NmuCI GTSAC 1 cut(s) 1004
NsiI ATGCAT 1 cut(s) 1152
NspI RCATGY 4 cut(s) 22, 596, 1154, 1162
PaeI GCATGC 2 cut(s) 22, 596
PagI TCATGA 1 cut(s) 1296
PasI CCCWGGG 1 cut(s) 633
PceI AGGCCT 1 cut(s) 168
PciI ACATGT 1 cut(s) 1158
PcsI WCGNNNNNNNCGW 1 cut(s) 422
PctI GAATGC 1 cut(s) 1340
PfeI GAWTC 1 cut(s) 1182
PflMI CCANNNNNTGG 1 cut(s) 109
PfoI TCCNGGA 1 cut(s) 138
PkrI GCNGC 5 cut(s) 67, 70, 591, 829, 962
Ple19I CGATCG 1 cut(s) 627
PleI GAGTC 3 cut(s) 193, 1264, 1326
PpsI GAGTC 3 cut(s) 193, 1264, 1326
PscI ACATGT 1 cut(s) 1158
PshAI GACNNNNGTC 1 cut(s) 110
Psp6I CCWGG 5 cut(s) 138, 246, 632, 799, 1132
PspEI GGTNACC 1 cut(s) 176
PspGI CCWGG 5 cut(s) 138, 246, 632, 799, 1132
PspN4I GGNNCC 3 cut(s) 631, 687, 994
PspPI GGNCC 4 cut(s) 100, 629, 685, 794
PstI CTGCAG 2 cut(s) 70, 962
PsuI RGATCY 1 cut(s) 1114
PvuI CGATCG 1 cut(s) 627
RsaI GTAC 1 cut(s) 782
RsaNI GTAC 1 cut(s) 781
SaqAI TTAA 4 cut(s) 266, 300, 564, 1209
SatI GCNGC 5 cut(s) 66, 69, 590, 828, 961
Sau3AI GATC 9 cut(s) 78, 90, 276, 292, 360, 551, 624, 929, 1114
Sau96I GGNCC 4 cut(s) 100, 629, 685, 794
SchI GAGTC 3 cut(s) 193, 1264, 1326
ScrFI CCNGG 5 cut(s) 140, 248, 634, 801, 1134
SfcI CTRYAG 2 cut(s) 66, 958
SinI GGWCC 4 cut(s) 100, 629, 685, 794
SmlI CTYRAG 3 cut(s) 265, 299, 845
SmoI CTYRAG 3 cut(s) 265, 299, 845
SphI GCATGC 2 cut(s) 22, 596
SseBI AGGCCT 1 cut(s) 168
SsiI CCGC 1 cut(s) 921
SspMI CTAG 1 cut(s) 1112
StuI AGGCCT 1 cut(s) 168
StyD4I CCNGG 5 cut(s) 138, 246, 632, 799, 1132
StyI CCWWGG 1 cut(s) 831
TaaI ACNGT 4 cut(s) 88, 387, 909, 1176
TaqI TCGA 2 cut(s) 623, 928
TaqII GACCGA 1 cut(s) 617
TfiI GAWTC 1 cut(s) 1182
Tru1I TTAA 4 cut(s) 266, 300, 564, 1209
Tru9I TTAA 4 cut(s) 266, 300, 564, 1209
TscAI CASTG 6 cut(s) 93, 100, 286, 370, 811, 1009
TseFI GTSAC 1 cut(s) 1004
TseI GCWGC 5 cut(s) 65, 68, 589, 827, 960
Tsp45I GTSAC 1 cut(s) 1004
TspRI CASTG 6 cut(s) 93, 100, 286, 370, 811, 1009
Van91I CCANNNNNTGG 1 cut(s) 109
Vha464I CTTAAG 2 cut(s) 265, 299
VpaK11BI GGWCC 4 cut(s) 100, 629, 685, 794
XapI RAATTY 2 cut(s) 398, 743
XceI RCATGY 4 cut(s) 22, 596, 1154, 1162
XspI CTAG 1 cut(s) 1112
Zsp2I ATGCAT 1 cut(s) 1152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.