Rroxscaffold_5G00372460

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0009761
Physical Location & Seq
Reverse (-)
53881062 .. 53882564
1503 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00372460.1

Sequence Viewer

Length: 1104 bp
ATGTTTTCTCTAACAAGCATGCCTTCAACAACATCAGTTCTATCAACCTACACGGCATTTGCTGCTGCAGCAATGCTGATCAGGACAGTGATCAGTGAGCTCCAGACTCTGGTCGGCCAGTTCATACCTCAAAAACTTCAGGACAAAATCATTGCCACACTTGCAGGCCTATTCGGTAACCTTCCTAATTCTCAAATGACTCTTATCATTGATGAAAGCAATGGCCTCTCTATCAATGAAGTTTACCAGGCTTCGGAACTCTACTTAAGCACAAGAATCACTGCCTCAATTGATCAACTTAAGGTTTCCCAGTCCCCACGCCAAAACAACTTCTGTGTTACCATAAACAAAGGTGAGAAGCTAATTGACGAGTTCGAAGGAATGCAGTTGAGGTGGGAAGTTTATTGTTTTGAAACGAAACAGGCGAATATGGACTATGAAAGCTATTGCTCATCAACAGAGGTAGTAAAGCATAAGTCAATTCAGCTCAATTTCCACAAGCAATACAGAGAAAAGAACGAGTTGATTGCTGAATTGGACAGGTTTGTGAGGCGAAAGGAATTTTATGGCAGAGTAGGCAAGGCATGGAAACGAGGTTACTTGTTGTATGGTCCTCCTGGCACTGGAAAATCGAGCTTGATTGCAGCCATGGCTAATTACCTCAAGTTTGATATCTATGATTTGGAGCTTACATATTTTCACAGCAACTCTGAGCTTAATGATGACAGACAAGTTAGAGGAACCAACCCCAGTGACAGCCAGGATGATCGTGTTTACCACCAACTACAAGGACAAGATCCTGCATTGTTGAGACCAGGCCGGATGGATATGCATGTTCACATGTCCTACTGCACACCATGTGGATTCAAAACCCTTGCTCATAACTACTTTAAAGTAAAAACTCATCATCTCTTCAATGAAATTGAAAAATTCATAAAGGAGGTTGATACGACTCCAGCAGAGATTGCAGAAGAACTCATGAAAAGTGAAGACACCGATACAGCGCTGGGAGGACTAGTTGCATTCCTGAAGAAGAGGAAAGAGATGAACTGCAGTGCTGAAGCCAATGTTGAAGAAACAATAGAAAATAATGAGGAATACTAG

Protein Analysis

367

Amino Acids

41.95

Weight (kDa)

5.59

Isoelectric Point (pI)

38.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 42 - 136 2.1e-22 Domain associated at C-terminal with AAA
AAA PF00004 200 - 239 4.9e-06 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 284 - 352 4.8e-24 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 109
AclWI GGATC 1 cut(s) 791
AcoI YGGCCR 1 cut(s) 115
AcsI RAATTY 2 cut(s) 560, 929
AcuI CTGAAG 3 cut(s) 122, 1049, 1080
AdeI CACNNNGTG 1 cut(s) 860
AfeI AGCGCT 1 cut(s) 1005
AfiI CCNNNNNNNGG 3 cut(s) 109, 253, 623
AflII CTTAAG 2 cut(s) 265, 299
AflIII ACRYGT 1 cut(s) 840
AgsI TTSAA 6 cut(s) 27, 413, 868, 916, 926, 1073
AhlI ACTAGT 1 cut(s) 1015
AjnI CCWGG 4 cut(s) 246, 616, 759, 814
AluBI AGCT 7 cut(s) 100, 361, 444, 487, 636, 688, 715
AluI AGCT 7 cut(s) 100, 361, 444, 487, 636, 688, 715
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 1 cut(s) 805
AlwI GGATC 1 cut(s) 791
AlwNI CAGNNNCTG 1 cut(s) 109
Aor51HI AGCGCT 1 cut(s) 1005
AoxI GGCC 4 cut(s) 115, 166, 223, 817
ApeKI GCWGC 4 cut(s) 62, 65, 68, 644
ApoI RAATTY 2 cut(s) 560, 929
AspLEI GCGC 1 cut(s) 1006
AspS9I GGNCC 1 cut(s) 611
AsuHPI GGTGA 1 cut(s) 365
AsuII TTCGAA 1 cut(s) 375
AvaII GGWCC 1 cut(s) 611
BanII GRGCYC 1 cut(s) 102
BbsI GAAGAC 1 cut(s) 996
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 4 cut(s) 49, 52, 80, 656
BccI CCATC 1 cut(s) 817
BceAI ACGGC 1 cut(s) 69
BcgI CGANNNNNNTGC 2 cut(s) 509, 543
BciT130I CCWGG 4 cut(s) 248, 618, 761, 816
BclI TGATCA 3 cut(s) 78, 90, 292
BcoDI GTCTC 1 cut(s) 805
BcuI ACTAGT 1 cut(s) 1015
BfaI CTAG 2 cut(s) 1016, 1102
BfmI CTRYAG 2 cut(s) 66, 1051
BfoI RGCGCY 1 cut(s) 1007
BfrI CTTAAG 2 cut(s) 265, 299
BisI GCNGC 4 cut(s) 63, 66, 69, 645
BlsI GCNGC 4 cut(s) 64, 67, 70, 646
Bme1390I CCNGG 4 cut(s) 248, 618, 761, 816
Bme18I GGWCC 1 cut(s) 611
BmgT120I GGNCC 1 cut(s) 611
BmiI GGNNCC 1 cut(s) 742
BmrFI CCNGG 4 cut(s) 248, 618, 761, 816
BmrI ACTGGG 2 cut(s) 304, 744
BmuI ACTGGG 2 cut(s) 304, 744
BoxI GACNNNNGTC 1 cut(s) 110
BpiI GAAGAC 1 cut(s) 996
BpmI CTGGAG 2 cut(s) 86, 939
Bpu14I TTCGAA 1 cut(s) 375
BpuEI CTTGAG 1 cut(s) 647
BsaI GGTCTC 1 cut(s) 805
BsaJI CCNNGG 1 cut(s) 648
BsaXI ACNNNNNCTCC 2 cut(s) 1002, 1032
Bsc4I CCNNNNNNNGG 3 cut(s) 109, 253, 623
Bse1I ACTGG 4 cut(s) 118, 310, 628, 750
Bse3DI GCAATG 3 cut(s) 78, 150, 226
BseBI CCWGG 4 cut(s) 248, 618, 761, 816
BseDI CCNNGG 1 cut(s) 648
BseGI GGATG 2 cut(s) 769, 828
BseLI CCNNNNNNNGG 3 cut(s) 109, 253, 623
BseMI GCAATG 3 cut(s) 78, 150, 226
BseMII CTCAG 1 cut(s) 702
BseNI ACTGG 4 cut(s) 118, 310, 628, 750
BseXI GCAGC 4 cut(s) 49, 52, 80, 656
BseYI CCCAGC 1 cut(s) 1006
BsgI GTGCAG 1 cut(s) 835
BshFI GGCC 4 cut(s) 117, 168, 225, 819
BsiHKAI GWGCWC 1 cut(s) 102
BsiSI CCGG 1 cut(s) 820
BslFI GGGAC 1 cut(s) 298
BslI CCNNNNNNNGG 3 cut(s) 109, 253, 623
BsmAI GTCTC 1 cut(s) 805
BsmFI GGGAC 1 cut(s) 298
BsmI GAATGC 2 cut(s) 387, 1022
BsnI GGCC 4 cut(s) 117, 168, 225, 819
Bso31I GGTCTC 1 cut(s) 805
Bsp119I TTCGAA 1 cut(s) 375
Bsp1286I GDGCHC 1 cut(s) 102
Bsp143I GATC 5 cut(s) 78, 90, 292, 766, 796
Bsp19I CCATGG 1 cut(s) 648
BspANI GGCC 4 cut(s) 117, 168, 225, 819
BspCNI CTCAG 1 cut(s) 703
BspHI TCATGA 1 cut(s) 978
BspLI GGNNCC 1 cut(s) 742
BspMAI CTGCAG 2 cut(s) 70, 1055
BspPI GGATC 1 cut(s) 791
BspT104I TTCGAA 1 cut(s) 375
BspTI CTTAAG 2 cut(s) 265, 299
BspTNI GGTCTC 1 cut(s) 805
BsrDI GCAATG 3 cut(s) 78, 150, 226
BsrI ACTGG 4 cut(s) 118, 310, 628, 750
BssECI CCNNGG 1 cut(s) 648
BssMI GATC 5 cut(s) 78, 90, 292, 766, 796
BssT1I CCWWGG 1 cut(s) 648
Bst2UI CCWGG 4 cut(s) 248, 618, 761, 816
Bst4CI ACNGT 1 cut(s) 88
Bst6I CTCTTC 2 cut(s) 917, 1028
BstAFI CTTAAG 2 cut(s) 265, 299
BstAPI GCANNNNNTGC 2 cut(s) 62, 965
BstBI TTCGAA 1 cut(s) 375
BstC8I GCNNGC 2 cut(s) 20, 166
BstDEI CTNAG 1 cut(s) 711
BstDSI CCRYGG 1 cut(s) 648
BstEII GGTNACC 1 cut(s) 176
BstF5I GGATG 2 cut(s) 769, 828
BstH2I RGCGCY 1 cut(s) 1007
BstHHI GCGC 1 cut(s) 1006
BstKTI GATC 5 cut(s) 81, 93, 295, 769, 799
BstMAI GTCTC 1 cut(s) 805
BstMBI GATC 5 cut(s) 78, 90, 292, 766, 796
BstMWI GCNNNNNNNGC 6 cut(s) 62, 68, 161, 576, 650, 965
BstNI CCWGG 4 cut(s) 248, 618, 761, 816
BstNSI RCATGY 3 cut(s) 22, 836, 844
BstPAI GACNNNNGTC 1 cut(s) 110
BstPI GGTNACC 1 cut(s) 176
BstSCI CCNGG 4 cut(s) 246, 616, 759, 814
BstSFI CTRYAG 2 cut(s) 66, 1051
BstV1I GCAGC 4 cut(s) 49, 52, 80, 656
BstV2I GAAGAC 1 cut(s) 996
BstX2I RGATCY 1 cut(s) 796
BstYI RGATCY 1 cut(s) 796
BsuRI GGCC 4 cut(s) 117, 168, 225, 819
BtgI CCRYGG 1 cut(s) 648
BtsCI GGATG 2 cut(s) 769, 828
BtsI GCAGTG 2 cut(s) 279, 1060
BtsIMutI CAGTG 6 cut(s) 93, 100, 279, 621, 757, 1060
Cac8I GCNNGC 2 cut(s) 20, 166
CaiI CAGNNNCTG 1 cut(s) 109
CciI TCATGA 1 cut(s) 978
CfoI GCGC 1 cut(s) 1006
Cfr13I GGNCC 1 cut(s) 611
CviAII CATG 7 cut(s) 19, 585, 649, 833, 841, 858, 979
DdeI CTNAG 1 cut(s) 711
DpnI GATC 5 cut(s) 80, 92, 294, 768, 798
DpnII GATC 5 cut(s) 78, 90, 292, 766, 796
DraI TTTAAA 1 cut(s) 892
DraIII CACNNNGTG 1 cut(s) 860
EaeI YGGCCR 1 cut(s) 115
Eam1104I CTCTTC 2 cut(s) 917, 1028
EarI CTCTTC 2 cut(s) 917, 1028
Ecl136II GAGCTC 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 648
Eco147I AGGCCT 1 cut(s) 168
Eco24I GRGCYC 1 cut(s) 102
Eco31I GGTCTC 1 cut(s) 805
Eco32I GATATC 1 cut(s) 673
Eco47I GGWCC 1 cut(s) 611
Eco47III AGCGCT 1 cut(s) 1005
Eco53kI GAGCTC 1 cut(s) 100
Eco57I CTGAAG 3 cut(s) 122, 1049, 1080
Eco91I GGTNACC 1 cut(s) 176
EcoICRI GAGCTC 1 cut(s) 100
EcoO65I GGTNACC 1 cut(s) 176
EcoRII CCWGG 4 cut(s) 246, 616, 759, 814
EcoRV GATATC 1 cut(s) 673
EcoT14I CCWWGG 1 cut(s) 648
EcoT22I ATGCAT 1 cut(s) 834
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 648
FaeI CATG 7 cut(s) 22, 588, 652, 836, 844, 861, 982
FalI AAGNNNNNCTT 2 cut(s) 7, 39
FaqI GGGAC 1 cut(s) 298
FatI CATG 7 cut(s) 18, 584, 648, 832, 840, 857, 978
FbaI TGATCA 3 cut(s) 78, 90, 292
Fnu4HI GCNGC 4 cut(s) 63, 66, 69, 645
FokI GGATG 2 cut(s) 776, 835
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 4 cut(s) 63, 66, 69, 645
FspBI CTAG 2 cut(s) 1016, 1102
GlaI GCGC 1 cut(s) 1005
GluI GCNGC 4 cut(s) 63, 66, 69, 645
GsaI CCCAGC 1 cut(s) 1010
GsuI CTGGAG 2 cut(s) 86, 939
HaeII RGCGCY 1 cut(s) 1007
HaeIII GGCC 4 cut(s) 117, 168, 225, 819
HapII CCGG 1 cut(s) 820
HhaI GCGC 1 cut(s) 1006
Hin1II CATG 7 cut(s) 22, 588, 652, 836, 844, 861, 982
Hin6I GCGC 1 cut(s) 1004
HinP1I GCGC 1 cut(s) 1004
HinfI GANTC 5 cut(s) 106, 199, 276, 864, 952
HpaII CCGG 1 cut(s) 820
HphI GGTGA 1 cut(s) 365
Hpy166II GTNNAC 3 cut(s) 244, 775, 838
Hpy188I TCNGA 2 cut(s) 256, 712
Hpy188III TCNNGA 5 cut(s) 82, 103, 140, 979, 1027
Hpy8I GTNNAC 3 cut(s) 244, 775, 838
HpyAV CCTTC 3 cut(s) 33, 191, 371
HpyCH4III ACNGT 1 cut(s) 88
HpyF10VI GCNNNNNNNGC 6 cut(s) 62, 68, 161, 576, 650, 965
HpyF3I CTNAG 1 cut(s) 711
Hsp92II CATG 7 cut(s) 22, 588, 652, 836, 844, 861, 982
HspAI GCGC 1 cut(s) 1004
Ksp22I TGATCA 3 cut(s) 78, 90, 292
Kzo9I GATC 5 cut(s) 78, 90, 292, 766, 796
LmnI GCTCC 2 cut(s) 105, 685
Lsp1109I GCAGC 4 cut(s) 49, 52, 80, 656
MaeI CTAG 2 cut(s) 1016, 1102
MaeIII GTNAC 4 cut(s) 176, 337, 596, 752
MalI GATC 5 cut(s) 80, 92, 294, 768, 798
MboI GATC 5 cut(s) 78, 90, 292, 766, 796
MboII GAAGA 6 cut(s) 904, 983, 1001, 1042, 1045, 1085
MfeI CAATTG 1 cut(s) 288
MflI RGATCY 1 cut(s) 796
MhlI GDGCHC 1 cut(s) 102
MlyI GAGTC 3 cut(s) 100, 193, 946
Mph1103I ATGCAT 1 cut(s) 834
MseI TTAA 4 cut(s) 266, 300, 717, 891
MspCI CTTAAG 2 cut(s) 265, 299
MspI CCGG 1 cut(s) 820
MspR9I CCNGG 4 cut(s) 248, 618, 761, 816
MunI CAATTG 1 cut(s) 288
Mva1269I GAATGC 2 cut(s) 387, 1022
MvaI CCWGG 4 cut(s) 248, 618, 761, 816
MwoI GCNNNNNNNGC 6 cut(s) 62, 68, 161, 576, 650, 965
NcoI CCATGG 1 cut(s) 648
NdeII GATC 5 cut(s) 78, 90, 292, 766, 796
NlaIII CATG 7 cut(s) 22, 588, 652, 836, 844, 861, 982
NlaIV GGNNCC 1 cut(s) 742
NmuCI GTSAC 1 cut(s) 752
NsiI ATGCAT 1 cut(s) 834
NspI RCATGY 3 cut(s) 22, 836, 844
NspV TTCGAA 1 cut(s) 375
PaeI GCATGC 1 cut(s) 22
PagI TCATGA 1 cut(s) 978
PceI AGGCCT 1 cut(s) 168
PciI ACATGT 1 cut(s) 840
PcsI WCGNNNNNNNCGW 1 cut(s) 422
PctI GAATGC 2 cut(s) 387, 1022
PfeI GAWTC 2 cut(s) 276, 864
PflMI CCANNNNNTGG 1 cut(s) 109
PkrI GCNGC 4 cut(s) 64, 67, 70, 646
PleI GAGTC 3 cut(s) 100, 193, 946
PpsI GAGTC 3 cut(s) 100, 193, 946
PscI ACATGT 1 cut(s) 840
PshAI GACNNNNGTC 1 cut(s) 110
Psp124BI GAGCTC 1 cut(s) 102
Psp6I CCWGG 4 cut(s) 246, 616, 759, 814
PspEI GGTNACC 1 cut(s) 176
PspFI CCCAGC 1 cut(s) 1006
PspGI CCWGG 4 cut(s) 246, 616, 759, 814
PspN4I GGNNCC 1 cut(s) 742
PspPI GGNCC 1 cut(s) 611
PstI CTGCAG 2 cut(s) 70, 1055
PstNI CAGNNNCTG 1 cut(s) 109
PsuI RGATCY 1 cut(s) 796
SacI GAGCTC 1 cut(s) 102
SaqAI TTAA 4 cut(s) 266, 300, 717, 891
SatI GCNGC 4 cut(s) 63, 66, 69, 645
Sau3AI GATC 5 cut(s) 78, 90, 292, 766, 796
Sau96I GGNCC 1 cut(s) 611
SchI GAGTC 3 cut(s) 100, 193, 946
ScrFI CCNGG 4 cut(s) 248, 618, 761, 816
SduI GDGCHC 1 cut(s) 102
SfcI CTRYAG 2 cut(s) 66, 1051
SfuI TTCGAA 1 cut(s) 375
SinI GGWCC 1 cut(s) 611
SmlI CTYRAG 3 cut(s) 265, 299, 662
SmoI CTYRAG 3 cut(s) 265, 299, 662
SpeI ACTAGT 1 cut(s) 1015
SphI GCATGC 1 cut(s) 22
SseBI AGGCCT 1 cut(s) 168
SspMI CTAG 2 cut(s) 1016, 1102
SstI GAGCTC 1 cut(s) 102
StuI AGGCCT 1 cut(s) 168
StyD4I CCNGG 4 cut(s) 246, 616, 759, 814
StyI CCWWGG 1 cut(s) 648
TaaI ACNGT 1 cut(s) 88
TaqI TCGA 2 cut(s) 375, 632
TfiI GAWTC 2 cut(s) 276, 864
Tru1I TTAA 4 cut(s) 266, 300, 717, 891
Tru9I TTAA 4 cut(s) 266, 300, 717, 891
TscAI CASTG 6 cut(s) 93, 100, 286, 628, 757, 1060
TseFI GTSAC 1 cut(s) 752
TseI GCWGC 4 cut(s) 62, 65, 68, 644
Tsp45I GTSAC 1 cut(s) 752
TspDTI ATGAA 8 cut(s) 112, 228, 252, 453, 922, 933, 995, 1061
TspRI CASTG 6 cut(s) 93, 100, 286, 628, 757, 1060
Van91I CCANNNNNTGG 1 cut(s) 109
Vha464I CTTAAG 2 cut(s) 265, 299
VpaK11BI GGWCC 1 cut(s) 611
XapI RAATTY 2 cut(s) 560, 929
XceI RCATGY 3 cut(s) 22, 836, 844
XspI CTAG 2 cut(s) 1016, 1102
Zsp2I ATGCAT 1 cut(s) 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.