Rh7AG350200

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0009761
Physical Location & Seq
Forward (+)
44638330 .. 44639828
1499 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG350200.1

Sequence Viewer

Length: 1407 bp
ATGCCTTCAACAACATCAGTTCTATCAACCTACACCACATTTTCTGCTGCAGCAATGCTGATCAGGACAGTGATCAGTGAGGTCCAGACTATGGTCGGCCAGTTCATACCTCAAAAACTCCAGGACAAAATCATTGCCACACTTGCAGGCCTATTCGGTAACCTTCCTAATTCTCAAATGACTCTTATCATTGATGAAAACAATGGCCTCTCTATCAATGAAGTTTACCAGGCTTCAGAACTCTACTTAAGCACAAGGATCACTGCCTCAATTGATCAACTTAAGGTTTCCAAGTCCCCACGCCAAGACAACTTCTCTGTTACCATAAACAAAGGCGAGAAGCTCACTGACGAGTTTGAAGGAATACAGTTGAGGTGGGAATTCTGTTGTTCTGAAACGAAACAGGCGAATATGGACTATGAAAGCTATTGCTCATCAACAGAAGTAGTAGAGCATAAGTCAATTCAGCTCAGTTTCCACAAGCAATGCAGAGAAAAGGTATTAGAAACTTACCTGCCATATGTAGTGCAAAGATCAAAAGCAATTAAAGAGGAAAATAAGGTGGTGAGGCTGCATGCACTTGGAAACTTTGGTGGAGAATGTTCGATCGGTCCCTGGGGTTCAGTCATCTTTGAACATCCATCAACTTTTGACACATTGGCAATGGACCCAATGCTAAAGAAAGAGTTGATTGCTGACTTGGACAGGTTCGTGAAGCGAAAGGAATTTTATGGCAGAGTAGTCAAGGCATGGAAACGAGGGTACTTGTTGTATGGTCCTCCTGGCACTGGAAAGTCAAGCTTGATTGCAGCCATGGCTAATTACCTCAAGTTTGATATCTATGATTTGGAGCTTACATATTTACACAGCAACTCTGAGCTTAGGAGACTGTTAGTCTCCACCGCAAATCGATCAATCATAGTGATTGAGGATATTGACTGCAGCATCGAATTGAAGGACAGACAAGCTAGAGGAACCAACCCCAGTGACAGCCAGTTGACACTATCCGGATTGCTCAATTTCATTGATGGTTTATGGTCAAGCTGTGGAGATGAGAGGATAATTGTGTTTACCACCAACTACAAGGACAAGCTAGATCCTGCATTGTTGAGAGCAGGCCGAATGGATATGCATGTTCACATGTCCTACTGCACACCGTGTGGATTCAAAACCCTTGCTCATAACTACCTTAAAGTAAAAACTCATCATCTCTTCAATGAAATTGAAAAATTCATAAAGGAGGTTGATACAACTCCAGCAGAGATTGCAGAAGAACTCATGAAAAGTGAAGACACCGATGTAGCTGTGGAAGGACTCATTGCATTCCTGAAGAAGAAGAGAAAGATGAACTGCAATGCTGAAGCCAATGTTGAAGAAACAATAGAAAATAATGAACTAAAGGAATAA

Protein Analysis

468

Amino Acids

53.01

Weight (kDa)

5.81

Isoelectric Point (pI)

36.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 36 - 130 1.6e-23 Domain associated at C-terminal with AAA
AAA PF00004 255 - 382 1.2e-20 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 384 - 453 3.4e-24 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 522
AccB7I CCANNNNNTGG 1 cut(s) 91
AccIII TCCGGA 1 cut(s) 1007
AciI CCGC 1 cut(s) 903
AclWI GGATC 2 cut(s) 266, 1091
AcoI YGGCCR 1 cut(s) 97
AcsI RAATTY 3 cut(s) 380, 725, 1229
AcuI CTGAAG 3 cut(s) 219, 1349, 1380
AdeI CACNNNGTG 2 cut(s) 1158, 1160
AfaI GTAC 1 cut(s) 764
AfiI CCNNNNNNNGG 2 cut(s) 91, 788
AflII CTTAAG 2 cut(s) 247, 281
AflIII ACRYGT 1 cut(s) 1140
AgsI TTSAA 8 cut(s) 9, 359, 635, 955, 1168, 1216, 1226, 1373
AhdI GACNNNNNGTC 1 cut(s) 893
AjnI CCWGG 4 cut(s) 120, 228, 614, 781
Alw26I GTCTC 2 cut(s) 880, 901
AlwI GGATC 2 cut(s) 266, 1091
Aor13HI TCCGGA 1 cut(s) 1007
AoxI GGCC 4 cut(s) 97, 148, 205, 1117
ApeKI GCWGC 5 cut(s) 47, 50, 571, 809, 942
ApoI RAATTY 3 cut(s) 380, 725, 1229
AspS9I GGNCC 4 cut(s) 82, 611, 667, 776
AsuHPI GGTGA 1 cut(s) 577
AvaII GGWCC 4 cut(s) 82, 611, 667, 776
BbsI GAAGAC 1 cut(s) 1296
BbvI GCAGC 5 cut(s) 34, 62, 558, 821, 954
BccI CCATC 2 cut(s) 649, 1022
BciT130I CCWGG 4 cut(s) 122, 230, 616, 783
BclI TGATCA 3 cut(s) 60, 72, 274
BcoDI GTCTC 2 cut(s) 880, 901
BfaI CTAG 2 cut(s) 969, 1094
BfmI CTRYAG 2 cut(s) 48, 940
BfrI CTTAAG 2 cut(s) 247, 281
BfuAI ACCTGC 1 cut(s) 522
BisI GCNGC 5 cut(s) 48, 51, 572, 810, 943
BlsI GCNGC 5 cut(s) 49, 52, 573, 811, 944
Bme1390I CCNGG 4 cut(s) 122, 230, 616, 783
Bme18I GGWCC 4 cut(s) 82, 611, 667, 776
BmeRI GACNNNNNGTC 1 cut(s) 893
BmgT120I GGNCC 4 cut(s) 82, 611, 667, 776
BmiI GGNNCC 3 cut(s) 613, 669, 976
BmrFI CCNGG 4 cut(s) 122, 230, 616, 783
BmrI ACTGGG 1 cut(s) 978
BmsI GCATC 1 cut(s) 954
BmuI ACTGGG 1 cut(s) 978
BoxI GACNNNNGTC 1 cut(s) 92
BpiI GAAGAC 1 cut(s) 1296
BpmI CTGGAG 2 cut(s) 104, 1239
Bpu10I CCTNAGC 1 cut(s) 881
BpuEI CTTGAG 1 cut(s) 812
Bsa29I ATCGAT 1 cut(s) 910
BsaJI CCNNGG 3 cut(s) 614, 615, 813
BsaWI WCCGGW 1 cut(s) 1007
Bsc4I CCNNNNNNNGG 2 cut(s) 91, 788
Bse1I ACTGG 4 cut(s) 100, 793, 984, 994
Bse3DI GCAATG 6 cut(s) 60, 132, 491, 669, 1317, 1360
BseAI TCCGGA 1 cut(s) 1007
BseBI CCWGG 4 cut(s) 122, 230, 616, 783
BseCI ATCGAT 1 cut(s) 910
BseDI CCNNGG 3 cut(s) 614, 615, 813
BseGI GGATG 1 cut(s) 637
BseLI CCNNNNNNNGG 2 cut(s) 91, 788
BseMI GCAATG 6 cut(s) 60, 132, 491, 669, 1317, 1360
BseMII CTCAG 2 cut(s) 484, 867
BseNI ACTGG 4 cut(s) 100, 793, 984, 994
BseXI GCAGC 5 cut(s) 34, 62, 558, 821, 954
BsgI GTGCAG 1 cut(s) 1135
Bsh1285I CGRYCG 1 cut(s) 609
BshFI GGCC 4 cut(s) 99, 150, 207, 1119
BshVI ATCGAT 1 cut(s) 910
BsiEI CGRYCG 1 cut(s) 609
BsiSI CCGG 1 cut(s) 1008
BslFI GGGAC 2 cut(s) 280, 597
BslI CCNNNNNNNGG 2 cut(s) 91, 788
BsmAI GTCTC 2 cut(s) 880, 901
BsmFI GGGAC 2 cut(s) 280, 597
BsmI GAATGC 1 cut(s) 1322
BsnI GGCC 4 cut(s) 99, 150, 207, 1119
Bsp13I TCCGGA 1 cut(s) 1007
Bsp143I GATC 8 cut(s) 60, 72, 258, 274, 533, 606, 911, 1096
Bsp19I CCATGG 1 cut(s) 813
BspACI CCGC 1 cut(s) 903
BspANI GGCC 4 cut(s) 99, 150, 207, 1119
BspCNI CTCAG 2 cut(s) 483, 868
BspDI ATCGAT 1 cut(s) 910
BspEI TCCGGA 1 cut(s) 1007
BspHI TCATGA 1 cut(s) 1278
BspLI GGNNCC 3 cut(s) 613, 669, 976
BspMAI CTGCAG 2 cut(s) 52, 944
BspMI ACCTGC 1 cut(s) 522
BspPI GGATC 2 cut(s) 266, 1091
BspTI CTTAAG 2 cut(s) 247, 281
BsrDI GCAATG 6 cut(s) 60, 132, 491, 669, 1317, 1360
BsrI ACTGG 4 cut(s) 100, 793, 984, 994
BssECI CCNNGG 3 cut(s) 614, 615, 813
BssMI GATC 8 cut(s) 60, 72, 258, 274, 533, 606, 911, 1096
BssT1I CCWWGG 1 cut(s) 813
Bst2UI CCWGG 4 cut(s) 122, 230, 616, 783
Bst4CI ACNGT 4 cut(s) 70, 369, 891, 1158
Bst6I CTCTTC 2 cut(s) 1217, 1331
BstAFI CTTAAG 2 cut(s) 247, 281
BstAPI GCANNNNNTGC 1 cut(s) 1265
BstC8I GCNNGC 3 cut(s) 148, 576, 1117
BstDEI CTNAG 3 cut(s) 470, 876, 881
BstDSI CCRYGG 1 cut(s) 813
BstEII GGTNACC 1 cut(s) 158
BstF5I GGATG 1 cut(s) 637
BstKTI GATC 8 cut(s) 63, 75, 261, 277, 536, 609, 914, 1099
BstMAI GTCTC 2 cut(s) 880, 901
BstMBI GATC 8 cut(s) 60, 72, 258, 274, 533, 606, 911, 1096
BstMCI CGRYCG 1 cut(s) 609
BstMWI GCNNNNNNNGC 3 cut(s) 143, 815, 1265
BstNI CCWGG 4 cut(s) 122, 230, 616, 783
BstNSI RCATGY 3 cut(s) 578, 1136, 1144
BstPAI GACNNNNGTC 1 cut(s) 92
BstPI GGTNACC 1 cut(s) 158
BstSCI CCNGG 4 cut(s) 120, 228, 614, 781
BstSFI CTRYAG 2 cut(s) 48, 940
BstV1I GCAGC 5 cut(s) 34, 62, 558, 821, 954
BstV2I GAAGAC 1 cut(s) 1296
BstX2I RGATCY 1 cut(s) 1096
BstYI RGATCY 1 cut(s) 1096
Bsu15I ATCGAT 1 cut(s) 910
BsuRI GGCC 4 cut(s) 99, 150, 207, 1119
BsuTUI ATCGAT 1 cut(s) 910
BtgI CCRYGG 1 cut(s) 813
BtsCI GGATG 1 cut(s) 637
BtsI GCAGTG 1 cut(s) 261
BtsIMutI CAGTG 6 cut(s) 75, 82, 261, 345, 786, 991
BveI ACCTGC 1 cut(s) 522
Cac8I GCNNGC 3 cut(s) 148, 576, 1117
CciI TCATGA 1 cut(s) 1278
Cfr13I GGNCC 4 cut(s) 82, 611, 667, 776
ClaI ATCGAT 1 cut(s) 910
Csp6I GTAC 1 cut(s) 763
CviAII CATG 6 cut(s) 575, 750, 814, 1133, 1141, 1279
CviQI GTAC 1 cut(s) 763
DdeI CTNAG 3 cut(s) 470, 876, 881
DpnI GATC 8 cut(s) 62, 74, 260, 276, 535, 608, 913, 1098
DpnII GATC 8 cut(s) 60, 72, 258, 274, 533, 606, 911, 1096
DraIII CACNNNGTG 2 cut(s) 1158, 1160
DriI GACNNNNNGTC 1 cut(s) 893
EaeI YGGCCR 1 cut(s) 97
Eam1104I CTCTTC 2 cut(s) 1217, 1331
Eam1105I GACNNNNNGTC 1 cut(s) 893
EarI CTCTTC 2 cut(s) 1217, 1331
Eco130I CCWWGG 1 cut(s) 813
Eco147I AGGCCT 1 cut(s) 150
Eco32I GATATC 1 cut(s) 838
Eco47I GGWCC 4 cut(s) 82, 611, 667, 776
Eco57I CTGAAG 3 cut(s) 219, 1349, 1380
Eco91I GGTNACC 1 cut(s) 158
EcoO65I GGTNACC 1 cut(s) 158
EcoRI GAATTC 1 cut(s) 380
EcoRII CCWGG 4 cut(s) 120, 228, 614, 781
EcoRV GATATC 1 cut(s) 838
EcoT14I CCWWGG 1 cut(s) 813
EcoT22I ATGCAT 1 cut(s) 1134
ErhI CCWWGG 1 cut(s) 813
FaeI CATG 6 cut(s) 578, 753, 817, 1136, 1144, 1282
FalI AAGNNNNNCTT 2 cut(s) 785, 817
FaqI GGGAC 2 cut(s) 280, 597
FatI CATG 6 cut(s) 574, 749, 813, 1132, 1140, 1278
FauNDI CATATG 1 cut(s) 520
FbaI TGATCA 3 cut(s) 60, 72, 274
Fnu4HI GCNGC 5 cut(s) 48, 51, 572, 810, 943
FokI GGATG 1 cut(s) 624
Fsp4HI GCNGC 5 cut(s) 48, 51, 572, 810, 943
FspBI CTAG 2 cut(s) 969, 1094
GluI GCNGC 5 cut(s) 48, 51, 572, 810, 943
GsuI CTGGAG 2 cut(s) 104, 1239
HaeIII GGCC 4 cut(s) 99, 150, 207, 1119
HapII CCGG 1 cut(s) 1008
Hin1II CATG 6 cut(s) 578, 753, 817, 1136, 1144, 1282
HincII GTYRAC 1 cut(s) 999
HindII GTYRAC 1 cut(s) 999
HindIII AAGCTT 1 cut(s) 799
HinfI GANTC 3 cut(s) 181, 1164, 1314
HpaII CCGG 1 cut(s) 1008
HphI GGTGA 1 cut(s) 577
Hpy166II GTNNAC 4 cut(s) 226, 999, 1071, 1138
Hpy188I TCNGA 3 cut(s) 238, 394, 877
Hpy188III TCNNGA 6 cut(s) 64, 85, 712, 1008, 1279, 1327
Hpy8I GTNNAC 4 cut(s) 226, 999, 1071, 1138
HpyAV CCTTC 5 cut(s) 15, 173, 353, 949, 1304
HpyCH4III ACNGT 4 cut(s) 70, 369, 891, 1158
HpyF10VI GCNNNNNNNGC 3 cut(s) 143, 815, 1265
HpyF3I CTNAG 3 cut(s) 470, 876, 881
Hsp92II CATG 6 cut(s) 578, 753, 817, 1136, 1144, 1282
Kpn2I TCCGGA 1 cut(s) 1007
Ksp22I TGATCA 3 cut(s) 60, 72, 274
Kzo9I GATC 8 cut(s) 60, 72, 258, 274, 533, 606, 911, 1096
LmnI GCTCC 1 cut(s) 850
Lsp1109I GCAGC 5 cut(s) 34, 62, 558, 821, 954
LweI GCATC 1 cut(s) 954
MaeI CTAG 2 cut(s) 969, 1094
MaeIII GTNAC 3 cut(s) 158, 319, 986
MalI GATC 8 cut(s) 62, 74, 260, 276, 535, 608, 913, 1098
MboI GATC 8 cut(s) 60, 72, 258, 274, 533, 606, 911, 1096
MboII GAAGA 7 cut(s) 1204, 1283, 1301, 1342, 1345, 1348, 1385
MfeI CAATTG 1 cut(s) 270
MflI RGATCY 1 cut(s) 1096
MlyI GAGTC 2 cut(s) 175, 1308
Mph1103I ATGCAT 1 cut(s) 1134
MroI TCCGGA 1 cut(s) 1007
MseI TTAA 4 cut(s) 248, 282, 546, 1191
MspCI CTTAAG 2 cut(s) 247, 281
MspI CCGG 1 cut(s) 1008
MspR9I CCNGG 4 cut(s) 122, 230, 616, 783
MunI CAATTG 1 cut(s) 270
Mva1269I GAATGC 1 cut(s) 1322
MvaI CCWGG 4 cut(s) 122, 230, 616, 783
MwoI GCNNNNNNNGC 3 cut(s) 143, 815, 1265
NcoI CCATGG 1 cut(s) 813
NdeI CATATG 1 cut(s) 520
NdeII GATC 8 cut(s) 60, 72, 258, 274, 533, 606, 911, 1096
NlaIII CATG 6 cut(s) 578, 753, 817, 1136, 1144, 1282
NlaIV GGNNCC 3 cut(s) 613, 669, 976
NmuCI GTSAC 1 cut(s) 986
NsiI ATGCAT 1 cut(s) 1134
NspI RCATGY 3 cut(s) 578, 1136, 1144
PaeI GCATGC 1 cut(s) 578
PagI TCATGA 1 cut(s) 1278
PasI CCCWGGG 1 cut(s) 615
PceI AGGCCT 1 cut(s) 150
PciI ACATGT 1 cut(s) 1140
PcsI WCGNNNNNNNCGW 1 cut(s) 404
PctI GAATGC 1 cut(s) 1322
PfeI GAWTC 1 cut(s) 1164
PflMI CCANNNNNTGG 1 cut(s) 91
PfoI TCCNGGA 1 cut(s) 120
PkrI GCNGC 5 cut(s) 49, 52, 573, 811, 944
Ple19I CGATCG 1 cut(s) 609
PleI GAGTC 2 cut(s) 175, 1308
PpsI GAGTC 2 cut(s) 175, 1308
PscI ACATGT 1 cut(s) 1140
PshAI GACNNNNGTC 1 cut(s) 92
Psp6I CCWGG 4 cut(s) 120, 228, 614, 781
PspEI GGTNACC 1 cut(s) 158
PspGI CCWGG 4 cut(s) 120, 228, 614, 781
PspN4I GGNNCC 3 cut(s) 613, 669, 976
PspPI GGNCC 4 cut(s) 82, 611, 667, 776
PstI CTGCAG 2 cut(s) 52, 944
PsuI RGATCY 1 cut(s) 1096
PvuI CGATCG 1 cut(s) 609
RsaI GTAC 1 cut(s) 764
RsaNI GTAC 1 cut(s) 763
SaqAI TTAA 4 cut(s) 248, 282, 546, 1191
SatI GCNGC 5 cut(s) 48, 51, 572, 810, 943
Sau3AI GATC 8 cut(s) 60, 72, 258, 274, 533, 606, 911, 1096
Sau96I GGNCC 4 cut(s) 82, 611, 667, 776
SchI GAGTC 2 cut(s) 175, 1308
ScrFI CCNGG 4 cut(s) 122, 230, 616, 783
SfaNI GCATC 1 cut(s) 954
SfcI CTRYAG 2 cut(s) 48, 940
SinI GGWCC 4 cut(s) 82, 611, 667, 776
SmlI CTYRAG 3 cut(s) 247, 281, 827
SmoI CTYRAG 3 cut(s) 247, 281, 827
SphI GCATGC 1 cut(s) 578
SseBI AGGCCT 1 cut(s) 150
SsiI CCGC 1 cut(s) 903
SspMI CTAG 2 cut(s) 969, 1094
StuI AGGCCT 1 cut(s) 150
StyD4I CCNGG 4 cut(s) 120, 228, 614, 781
StyI CCWWGG 1 cut(s) 813
TaaI ACNGT 4 cut(s) 70, 369, 891, 1158
TaqI TCGA 3 cut(s) 605, 910, 948
TaqII GACCGA 1 cut(s) 599
TfiI GAWTC 1 cut(s) 1164
Tru1I TTAA 4 cut(s) 248, 282, 546, 1191
Tru9I TTAA 4 cut(s) 248, 282, 546, 1191
TscAI CASTG 6 cut(s) 75, 82, 268, 352, 793, 991
TseFI GTSAC 1 cut(s) 986
TseI GCWGC 5 cut(s) 47, 50, 571, 809, 942
Tsp45I GTSAC 1 cut(s) 986
TspRI CASTG 6 cut(s) 75, 82, 268, 352, 793, 991
Van91I CCANNNNNTGG 1 cut(s) 91
Vha464I CTTAAG 2 cut(s) 247, 281
VpaK11BI GGWCC 4 cut(s) 82, 611, 667, 776
XapI RAATTY 3 cut(s) 380, 725, 1229
XceI RCATGY 3 cut(s) 578, 1136, 1144
XspI CTAG 2 cut(s) 969, 1094
Zsp2I ATGCAT 1 cut(s) 1134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.