RchiOBHm_Chr7g0225641

RNA polymerase II subunit 5-mediating protein homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0002516
Physical Location & Seq
Forward (+)
48481968 .. 48489685
7718 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ20209

Sequence Viewer

Length: 1107 bp
ATGAGAGGCAAAGGCACGGTGACGCCTCTATCCTCTGTCTTCTCAGTGGAAGACACACAGAAAGCAGCGAAGCGCGTGCAGGACACGATCGCCCAGAAGGACCTCGAGCTCGACCACCTCCGTGGCTTCGCCGCCGACAACACAGACCTCATTAACCTCGTCGCCCGACTCCCCGAACACCTCCACCACGACATTATGGTTCCGTTTGGGAAGGCAGCATTTTTCCCTGGGCGGTTGGTGCACACCAATGAGTTTATGGTTCTACTGGGAGAAGGTTACTATGCCGAAAGGACTTCGAAGCAAACAGTAGATATTTTGAACAGGAGAGGGAAGGTATTGGAGTCCCAAGTCGATTCGCTCAAGGCCATGGTTCAGGATCTCAAAGCTGAGGCTTCCTTTTTCAATGCTACTGCCACCGAAGCAGCGGAGGGCCTTGTAGAGATAGTGGAAGATTATGAGGAGGAAAGTTCTTCTGATAGAGAATGTACACCCAAGCAAGATTATGCTAATAATTCTAGAGCTGTAATTGCACAACCTGCTGATGAAGATGAAGAATTTGCTCGTATGATGGCCAGGATGGATGAACTTGAGAAAGAAGAACGTGAAGCTGAAAGTCATATGGCACAAAGTGATGAGGATGAGCAACTTCAGCATGGTTTGCATCAGCTTTCTGATCAGAAATCTCTCCATGGGAATCTCAAAATTTCGGAGTACCAGTCAAGGAAACCTCCAGAGCAGACAAAAGATGGAATTGCTTCTAATGAAGATAGTTTTACTGATGTGCCAAGTGGTTCGGGTCTGGTATCTAAATATGAAGATAAAGTATCACATGAAAATATATTGGGCCGTGATGTAAAAAGTGTTGCTGAGAAAGATTTGGTGTCTCCGGTAGTTGAGAATGTTCAGACTGTGCCTTTATCAAGAAGTCAGGTTTCTCTTGAAAGTTCAAAACCAAAATTTGACAGGGAGAAAGCTTTTACAGGTTCTATTGTGGAGCACGCTGATAATCTACAGATAAAATCAAGGGAAAGAACTACGGCTATATCGCAGTCCTCTGCTTCTCAAGTTTCAAAGCCAGTTTCAAGATTCAAGATGCAGAGAAAGTAG

Protein Analysis

368

Amino Acids

41.13

Weight (kDa)

5.14

Isoelectric Point (pI)

45.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prefoldin PF02996 29 - 141 4.6e-20 Prefoldin subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 544
AccII CGCG 1 cut(s) 75
AciI CCGC 3 cut(s) 132, 232, 425
AclWI GGATC 1 cut(s) 384
AcoI YGGCCR 1 cut(s) 570
AcsI RAATTY 3 cut(s) 554, 702, 956
AcuI CTGAAG 1 cut(s) 632
AcyI GRCGYC 1 cut(s) 23
AdeI CACNNNGTG 1 cut(s) 629
AfaI GTAC 2 cut(s) 487, 713
AgsI TTSAA 7 cut(s) 319, 403, 941, 948, 1071, 1083, 1090
AjnI CCWGG 2 cut(s) 226, 572
AleI CACNNNNGTG 1 cut(s) 120
AluBI AGCT 6 cut(s) 109, 386, 521, 608, 667, 974
AluI AGCT 6 cut(s) 109, 386, 521, 608, 667, 974
Alw21I GWGCWC 3 cut(s) 111, 243, 999
Alw26I GTCTC 1 cut(s) 888
Alw44I GTGCAC 1 cut(s) 239
AlwI GGATC 1 cut(s) 384
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 4 cut(s) 363, 430, 570, 844
ApaLI GTGCAC 1 cut(s) 239
ApeKI GCWGC 3 cut(s) 65, 215, 422
ApoI RAATTY 3 cut(s) 554, 702, 956
Asp700I GAANNNNTTC 1 cut(s) 754
AspLEI GCGC 1 cut(s) 75
AspS9I GGNCC 3 cut(s) 100, 430, 844
AsuHPI GGTGA 1 cut(s) 31
AsuII TTCGAA 1 cut(s) 296
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 1 cut(s) 100
BaeGI GKGCMC 1 cut(s) 243
BalI TGGCCA 1 cut(s) 572
BanII GRGCYC 1 cut(s) 111
BbsI GAAGAC 2 cut(s) 31, 57
Bbv12I GWGCWC 3 cut(s) 111, 243, 999
BbvCI CCTCAGC 1 cut(s) 387
BbvI GCAGC 3 cut(s) 77, 227, 434
BccI CCATC 3 cut(s) 562, 571, 740
BceAI ACGGC 2 cut(s) 831, 1053
BcgI CGANNNNNNTGC 2 cut(s) 58, 92
BciT130I CCWGG 2 cut(s) 228, 574
BclI TGATCA 1 cut(s) 673
BcoDI GTCTC 1 cut(s) 888
BfaI CTAG 1 cut(s) 516
BfmI CTRYAG 1 cut(s) 1010
BfuAI ACCTGC 1 cut(s) 544
BisI GCNGC 4 cut(s) 66, 132, 216, 423
BlsI GCNGC 4 cut(s) 67, 133, 217, 424
Bme1390I CCNGG 2 cut(s) 228, 574
Bme18I GGWCC 1 cut(s) 100
BmeT110I CYCGRG 1 cut(s) 104
BmgT120I GGNCC 3 cut(s) 100, 430, 844
BmiI GGNNCC 1 cut(s) 201
BmrFI CCNGG 2 cut(s) 228, 574
BmrI ACTGGG 1 cut(s) 275
BmsI GCATC 2 cut(s) 670, 1083
BmuI ACTGGG 1 cut(s) 275
BpiI GAAGAC 2 cut(s) 31, 57
BpmI CTGGAG 1 cut(s) 714
Bpu10I CCTNAGC 1 cut(s) 387
Bpu14I TTCGAA 1 cut(s) 296
BpuEI CTTGAG 3 cut(s) 344, 608, 1047
BsaHI GRCGYC 1 cut(s) 23
BsaJI CCNNGG 5 cut(s) 121, 226, 227, 366, 688
BsaWI WCCGGW 1 cut(s) 886
BsaXI ACNNNNNCTCC 2 cut(s) 701, 731
Bse1I ACTGG 3 cut(s) 270, 715, 1076
BseBI CCWGG 2 cut(s) 228, 574
BseDI CCNNGG 5 cut(s) 121, 226, 227, 366, 688
BseGI GGATG 3 cut(s) 582, 586, 643
BseMII CTCAG 3 cut(s) 57, 378, 858
BseNI ACTGG 3 cut(s) 270, 715, 1076
BseRI GAGGAG 1 cut(s) 473
BseSI GKGCMC 1 cut(s) 243
BseXI GCAGC 3 cut(s) 77, 227, 434
BsgI GTGCAG 1 cut(s) 98
Bsh1236I CGCG 1 cut(s) 75
Bsh1285I CGRYCG 1 cut(s) 90
BshFI GGCC 4 cut(s) 365, 432, 572, 846
BsiEI CGRYCG 1 cut(s) 90
BsiHKAI GWGCWC 3 cut(s) 111, 243, 999
BsiHKCI CYCGRG 1 cut(s) 104
BsiSI CCGG 1 cut(s) 887
BslFI GGGAC 1 cut(s) 328
BsmAI GTCTC 1 cut(s) 888
BsmFI GGGAC 1 cut(s) 328
BsnI GGCC 4 cut(s) 365, 432, 572, 846
BsoBI CYCGRG 1 cut(s) 104
Bsp119I TTCGAA 1 cut(s) 296
Bsp1286I GDGCHC 3 cut(s) 111, 243, 999
Bsp1407I TGTACA 1 cut(s) 485
Bsp143I GATC 3 cut(s) 87, 376, 673
Bsp19I CCATGG 2 cut(s) 366, 688
BspACI CCGC 3 cut(s) 132, 232, 425
BspANI GGCC 4 cut(s) 365, 432, 572, 846
BspCNI CTCAG 3 cut(s) 56, 379, 859
BspFNI CGCG 1 cut(s) 75
BspLI GGNNCC 1 cut(s) 201
BspMI ACCTGC 1 cut(s) 544
BspPI GGATC 1 cut(s) 384
BspT104I TTCGAA 1 cut(s) 296
BsrGI TGTACA 1 cut(s) 485
BsrI ACTGG 3 cut(s) 270, 715, 1076
BssECI CCNNGG 5 cut(s) 121, 226, 227, 366, 688
BssMI GATC 3 cut(s) 87, 376, 673
BssNI GRCGYC 1 cut(s) 23
BssT1I CCWWGG 2 cut(s) 366, 688
Bst2UI CCWGG 2 cut(s) 228, 574
Bst4CI ACNGT 3 cut(s) 19, 307, 910
BstACI GRCGYC 1 cut(s) 23
BstAPI GCANNNNNTGC 2 cut(s) 536, 658
BstAUI TGTACA 1 cut(s) 485
BstBI TTCGAA 1 cut(s) 296
BstC8I GCNNGC 2 cut(s) 77, 999
BstDEI CTNAG 3 cut(s) 43, 387, 867
BstDSI CCRYGG 3 cut(s) 121, 366, 688
BstF5I GGATG 3 cut(s) 582, 586, 643
BstFNI CGCG 1 cut(s) 75
BstHHI GCGC 1 cut(s) 75
BstKTI GATC 3 cut(s) 90, 379, 676
BstMAI GTCTC 1 cut(s) 888
BstMBI GATC 3 cut(s) 87, 376, 673
BstMCI CGRYCG 1 cut(s) 90
BstMWI GCNNNNNNNGC 6 cut(s) 238, 419, 527, 536, 649, 658
BstNI CCWGG 2 cut(s) 228, 574
BstSCI CCNGG 2 cut(s) 226, 572
BstSFI CTRYAG 1 cut(s) 1010
BstSLI GKGCMC 1 cut(s) 243
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 3 cut(s) 77, 227, 434
BstV2I GAAGAC 2 cut(s) 31, 57
BstX2I RGATCY 1 cut(s) 376
BstXI CCANNNNNNTGG 1 cut(s) 122
BstYI RGATCY 1 cut(s) 376
BsuRI GGCC 4 cut(s) 365, 432, 572, 846
BtgI CCRYGG 3 cut(s) 121, 366, 688
BtsCI GGATG 3 cut(s) 582, 586, 643
BtsIMutI CAGTG 1 cut(s) 51
BveI ACCTGC 1 cut(s) 544
Cac8I GCNNGC 2 cut(s) 77, 999
CfoI GCGC 1 cut(s) 75
Cfr13I GGNCC 3 cut(s) 100, 430, 844
CseI GACGC 1 cut(s) 31
Csp6I GTAC 2 cut(s) 486, 712
CviAII CATG 4 cut(s) 367, 653, 689, 830
CviQI GTAC 2 cut(s) 486, 712
DdeI CTNAG 3 cut(s) 43, 387, 867
DpnI GATC 3 cut(s) 89, 378, 675
DpnII GATC 3 cut(s) 87, 376, 673
DraIII CACNNNGTG 1 cut(s) 629
EaeI YGGCCR 1 cut(s) 570
Ecl136II GAGCTC 1 cut(s) 109
Eco130I CCWWGG 2 cut(s) 366, 688
Eco24I GRGCYC 1 cut(s) 111
Eco47I GGWCC 1 cut(s) 100
Eco53kI GAGCTC 1 cut(s) 109
Eco57I CTGAAG 1 cut(s) 632
Eco88I CYCGRG 1 cut(s) 104
EcoICRI GAGCTC 1 cut(s) 109
EcoO109I RGGNCCY 2 cut(s) 100, 430
EcoRII CCWGG 2 cut(s) 226, 572
EcoT14I CCWWGG 2 cut(s) 366, 688
EcoT38I GRGCYC 1 cut(s) 111
ErhI CCWWGG 2 cut(s) 366, 688
FaeI CATG 4 cut(s) 370, 656, 692, 833
FaqI GGGAC 1 cut(s) 328
FatI CATG 4 cut(s) 366, 652, 688, 829
FauNDI CATATG 1 cut(s) 618
FbaI TGATCA 1 cut(s) 673
Fnu4HI GCNGC 4 cut(s) 66, 132, 216, 423
FokI GGATG 3 cut(s) 589, 593, 650
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 4 cut(s) 66, 132, 216, 423
FspBI CTAG 1 cut(s) 516
GlaI GCGC 1 cut(s) 74
GluI GCNGC 4 cut(s) 66, 132, 216, 423
GsuI CTGGAG 1 cut(s) 714
HaeIII GGCC 4 cut(s) 365, 432, 572, 846
HapII CCGG 1 cut(s) 887
HgaI GACGC 1 cut(s) 31
HhaI GCGC 1 cut(s) 75
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 4 cut(s) 370, 656, 692, 833
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HindIII AAGCTT 1 cut(s) 972
HinfI GANTC 5 cut(s) 168, 341, 353, 694, 1086
HpaII CCGG 1 cut(s) 887
HphI GGTGA 1 cut(s) 31
Hpy166II GTNNAC 2 cut(s) 241, 488
Hpy188I TCNGA 5 cut(s) 475, 673, 678, 709, 906
Hpy188III TCNNGA 7 cut(s) 374, 516, 731, 921, 938, 1083, 1090
Hpy8I GTNNAC 2 cut(s) 241, 488
Hpy99I CGWCG 1 cut(s) 164
HpyAV CCTTC 4 cut(s) 91, 205, 266, 325
HpyCH4III ACNGT 3 cut(s) 19, 307, 910
HpyCH4IV ACGT 1 cut(s) 601
HpyCH4V TGCA 5 cut(s) 79, 241, 530, 661, 1096
HpyF10VI GCNNNNNNNGC 6 cut(s) 238, 419, 527, 536, 649, 658
HpyF3I CTNAG 3 cut(s) 43, 387, 867
HpySE526I ACGT 1 cut(s) 601
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 4 cut(s) 370, 656, 692, 833
HspAI GCGC 1 cut(s) 73
Ksp22I TGATCA 1 cut(s) 673
Kzo9I GATC 3 cut(s) 87, 376, 673
LmnI GCTCC 1 cut(s) 994
Lsp1109I GCAGC 3 cut(s) 77, 227, 434
LweI GCATC 2 cut(s) 670, 1083
MaeI CTAG 1 cut(s) 516
MaeII ACGT 1 cut(s) 601
MaeIII GTNAC 2 cut(s) 19, 275
MalI GATC 3 cut(s) 89, 378, 675
MboI GATC 3 cut(s) 87, 376, 673
MboII GAAGA 9 cut(s) 31, 62, 461, 462, 557, 563, 608, 776, 827
MflI RGATCY 1 cut(s) 376
MhlI GDGCHC 3 cut(s) 111, 243, 999
MlsI TGGCCA 1 cut(s) 572
MluCI AATT 6 cut(s) 511, 525, 554, 702, 750, 956
MluNI TGGCCA 1 cut(s) 572
MlyI GAGTC 2 cut(s) 162, 350
Mox20I TGGCCA 1 cut(s) 572
MroXI GAANNNNTTC 1 cut(s) 754
MscI TGGCCA 1 cut(s) 572
MseI TTAA 1 cut(s) 153
MslI CAYNNNNRTG 2 cut(s) 120, 246
Msp20I TGGCCA 1 cut(s) 572
MspA1I CMGCKG 1 cut(s) 425
MspI CCGG 1 cut(s) 887
MspR9I CCNGG 2 cut(s) 228, 574
MvaI CCWGG 2 cut(s) 228, 574
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 6 cut(s) 238, 419, 527, 536, 649, 658
NcoI CCATGG 2 cut(s) 366, 688
NdeI CATATG 1 cut(s) 618
NdeII GATC 3 cut(s) 87, 376, 673
NlaIII CATG 4 cut(s) 370, 656, 692, 833
NlaIV GGNNCC 1 cut(s) 201
NmuCI GTSAC 1 cut(s) 19
NspV TTCGAA 1 cut(s) 296
OliI CACNNNNGTG 1 cut(s) 120
PaeR7I CTCGAG 1 cut(s) 104
PasI CCCWGGG 1 cut(s) 227
PdmI GAANNNNTTC 1 cut(s) 754
PfeI GAWTC 3 cut(s) 353, 694, 1086
PkrI GCNGC 4 cut(s) 67, 133, 217, 424
Ple19I CGATCG 1 cut(s) 90
PleI GAGTC 2 cut(s) 162, 349
PpsI GAGTC 2 cut(s) 162, 349
PpuMI RGGWCCY 1 cut(s) 100
Psp124BI GAGCTC 1 cut(s) 111
Psp5II RGGWCCY 1 cut(s) 100
Psp6I CCWGG 2 cut(s) 226, 572
PspGI CCWGG 2 cut(s) 226, 572
PspN4I GGNNCC 1 cut(s) 201
PspPI GGNCC 3 cut(s) 100, 430, 844
PspPPI RGGWCCY 1 cut(s) 100
PspXI VCTCGAGB 1 cut(s) 104
PsuI RGATCY 1 cut(s) 376
PvuI CGATCG 1 cut(s) 90
RsaI GTAC 2 cut(s) 487, 713
RsaNI GTAC 2 cut(s) 486, 712
RseI CAYNNNNRTG 2 cut(s) 120, 246
SacI GAGCTC 1 cut(s) 111
SaqAI TTAA 1 cut(s) 153
SatI GCNGC 4 cut(s) 66, 132, 216, 423
Sau3AI GATC 3 cut(s) 87, 376, 673
Sau96I GGNCC 3 cut(s) 100, 430, 844
SchI GAGTC 2 cut(s) 162, 350
ScrFI CCNGG 2 cut(s) 228, 574
SduI GDGCHC 3 cut(s) 111, 243, 999
SfaNI GCATC 2 cut(s) 670, 1083
SfcI CTRYAG 1 cut(s) 1010
Sfr274I CTCGAG 1 cut(s) 104
SfuI TTCGAA 1 cut(s) 296
SinI GGWCC 1 cut(s) 100
SlaI CTCGAG 1 cut(s) 104
SmiMI CAYNNNNRTG 2 cut(s) 120, 246
SmlI CTYRAG 4 cut(s) 104, 359, 587, 1062
SmoI CTYRAG 4 cut(s) 104, 359, 587, 1062
Sse9I AATT 6 cut(s) 511, 525, 554, 702, 750, 956
SsiI CCGC 3 cut(s) 132, 232, 425
SspMI CTAG 1 cut(s) 516
SstI GAGCTC 1 cut(s) 111
StyD4I CCNGG 2 cut(s) 226, 572
StyI CCWWGG 2 cut(s) 366, 688
TaaI ACNGT 3 cut(s) 19, 307, 910
TaiI ACGT 1 cut(s) 604
TaqI TCGA 4 cut(s) 105, 111, 296, 351
TasI AATT 6 cut(s) 511, 525, 554, 702, 750, 956
TatI WGTACW 1 cut(s) 485
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 3 cut(s) 353, 694, 1086
Tru1I TTAA 1 cut(s) 153
Tru9I TTAA 1 cut(s) 153
TscAI CASTG 1 cut(s) 51
TseFI GTSAC 1 cut(s) 19
TseI GCWGC 3 cut(s) 65, 215, 422
Tsp45I GTSAC 1 cut(s) 19
TspDTI ATGAA 6 cut(s) 558, 564, 597, 777, 828, 846
TspGWI ACGGA 2 cut(s) 110, 192
TspRI CASTG 1 cut(s) 51
VneI GTGCAC 1 cut(s) 239
VpaK11BI GGWCC 1 cut(s) 100
XapI RAATTY 3 cut(s) 554, 702, 956
XbaI TCTAGA 1 cut(s) 515
XcmI CCANNNNNNNNNTGG 1 cut(s) 253
XhoI CTCGAG 1 cut(s) 104
XmnI GAANNNNTTC 1 cut(s) 754
XspI CTAG 1 cut(s) 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.