Rh4CG172700

RNA polymerase II subunit 5-mediating protein homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0002516
Physical Location & Seq
Forward (+)
37794925 .. 37801832
6908 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG172700.1

Sequence Viewer

Length: 1074 bp
ATGAAAGGCAAGGGGACGGTGACGCCTCTCTCTTCCATCTTCTCAGTGGAAGACACACAGAAAGCAGCGAAGCGCGTGCAGGACACGATCGCCCAAAATGACCTCGAGCTCGACCACCTCCGTGGCTTCGCCGCCGACAACACAGACCTCATTAACCTCGTCTCCCGACTCCCCGAACACCTCCACCACGACATTATGGTTCCATTTGGGAAGGCAGCATTTTTCCCAGGGCGTTTGGTGCACACCAATGAGTTTATGGTGAGGCTATTTAGGGTTTATCAATGCCAATTGGATGCTATAATTTTTCTTTGCTGCCAGGTTCTACTGGGAGAAAGTTACTATGCCGAAAGGACCTCGAAGCAAACAGTAGATATTTTGAACAGGAGAGGGAAGGTGTTGGAGTCCCAAGTCGATTCACTTAAGGCCATGGTTCAGGATCTCAAAGCTGAGGCTTCCTTTTTCAATGCTACTTGCTCCGAAGCGGTGGAGGGCCTTGTAGAGATAATGGAAGATTATGAGGAGGAAAGTTCTTCTGATAGAGAATGTGCACTCAAGCAAGATTCTCCTAATAATTCTGGAGCTGTAATTGCAAAACCTGCTGATGAAGATGAAGAATTTGCTCGTATGATGGCCAGGATGGATGAACTTGAGAAAGAAGAACGTGAAGCTGAAAGTCAGATGGCACAAAGTGATGAGGATGAGCAAGTTCAGCATGGTTTGCATCAGCTTTCTGATCAGAAATCTCTCCATGGGAATCTCAAAATTTCGGAGTACCAGTCAAGGAAACCTCCAGAGCAGACAAAAGATGGAGTTGCTTGTAATGAAGATAGTTTTACTGATGTGCCAAGTTGTTCGGGTCTGGAATCTATATCTGACGATAAAGTATCACATGAAAATATATTGGGCTGTGATGTAAAAAGTGTTGCTGAGAAAGATTTTGCGTCTCCAGTAGTTGAGAATGTTCAGACTGTGCCTTTATCAAGAAGTCAGGTATATAATGCTTCTCTTACATTCTCCATCAATTTTCCTGCTTTTCACGGTCTTGGCAGTTGGCATGCTTTCTTGCCAACATAG

Protein Analysis

357

Amino Acids

39.83

Weight (kDa)

4.72

Isoelectric Point (pI)

44.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prefoldin PF02996 107 - 162 1.2e-07 Prefoldin subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 604
AccII CGCG 1 cut(s) 75
AciI CCGC 2 cut(s) 132, 482
AclWI GGATC 1 cut(s) 444
AcoI YGGCCR 1 cut(s) 630
AcsI RAATTY 2 cut(s) 614, 762
AcyI GRCGYC 1 cut(s) 23
AdeI CACNNNGTG 1 cut(s) 689
AfaI GTAC 1 cut(s) 773
AflII CTTAAG 1 cut(s) 419
AgsI TTSAA 2 cut(s) 379, 463
AjnI CCWGG 3 cut(s) 226, 315, 632
AleI CACNNNNGTG 1 cut(s) 120
AluBI AGCT 5 cut(s) 109, 446, 581, 668, 727
AluI AGCT 5 cut(s) 109, 446, 581, 668, 727
Alw21I GWGCWC 3 cut(s) 111, 243, 550
Alw26I GTCTC 2 cut(s) 166, 948
Alw44I GTGCAC 2 cut(s) 239, 546
AlwI GGATC 1 cut(s) 444
Ama87I CYCGRG 1 cut(s) 104
AoxI GGCC 3 cut(s) 423, 490, 630
ApaLI GTGCAC 2 cut(s) 239, 546
ApeKI GCWGC 3 cut(s) 65, 215, 312
ApoI RAATTY 2 cut(s) 614, 762
AspLEI GCGC 1 cut(s) 75
AspS9I GGNCC 2 cut(s) 351, 490
AsuHPI GGTGA 2 cut(s) 31, 271
AvaI CYCGRG 1 cut(s) 104
AvaII GGWCC 1 cut(s) 351
BaeGI GKGCMC 2 cut(s) 243, 550
BalI TGGCCA 1 cut(s) 632
BanII GRGCYC 1 cut(s) 111
BbsI GAAGAC 1 cut(s) 57
Bbv12I GWGCWC 3 cut(s) 111, 243, 550
BbvCI CCTCAGC 1 cut(s) 447
BbvI GCAGC 3 cut(s) 77, 227, 299
BccI CCATC 6 cut(s) 44, 622, 631, 673, 800, 1025
BcgI CGANNNNNNTGC 2 cut(s) 58, 92
BciT130I CCWGG 3 cut(s) 228, 317, 634
BclI TGATCA 1 cut(s) 733
BcoDI GTCTC 2 cut(s) 166, 948
BfrI CTTAAG 1 cut(s) 419
BfuAI ACCTGC 1 cut(s) 604
BisI GCNGC 4 cut(s) 66, 132, 216, 313
BlsI GCNGC 4 cut(s) 67, 133, 217, 314
Bme1390I CCNGG 3 cut(s) 228, 317, 634
Bme18I GGWCC 1 cut(s) 351
BmeT110I CYCGRG 1 cut(s) 104
BmgT120I GGNCC 2 cut(s) 351, 490
BmiI GGNNCC 1 cut(s) 201
BmrFI CCNGG 3 cut(s) 228, 317, 634
BmrI ACTGGG 1 cut(s) 335
BmsI GCATC 2 cut(s) 283, 730
BmuI ACTGGG 1 cut(s) 335
BpiI GAAGAC 1 cut(s) 57
BpmI CTGGAG 3 cut(s) 597, 774, 930
Bpu10I CCTNAGC 1 cut(s) 447
BpuEI CTTGAG 2 cut(s) 536, 668
BsaHI GRCGYC 1 cut(s) 23
BsaJI CCNNGG 5 cut(s) 121, 226, 227, 426, 748
BsaXI ACNNNNNCTCC 4 cut(s) 146, 176, 761, 791
Bse1I ACTGG 3 cut(s) 330, 775, 947
BseBI CCWGG 3 cut(s) 228, 317, 634
BseDI CCNNGG 5 cut(s) 121, 226, 227, 426, 748
BseGI GGATG 4 cut(s) 298, 642, 646, 703
BseMII CTCAG 3 cut(s) 57, 438, 918
BseNI ACTGG 3 cut(s) 330, 775, 947
BseRI GAGGAG 1 cut(s) 533
BseSI GKGCMC 2 cut(s) 243, 550
BseXI GCAGC 3 cut(s) 77, 227, 299
BsgI GTGCAG 1 cut(s) 98
Bsh1236I CGCG 1 cut(s) 75
Bsh1285I CGRYCG 1 cut(s) 90
BshFI GGCC 3 cut(s) 425, 492, 632
BsiEI CGRYCG 1 cut(s) 90
BsiHKAI GWGCWC 3 cut(s) 111, 243, 550
BsiHKCI CYCGRG 1 cut(s) 104
BslFI GGGAC 2 cut(s) 28, 388
BsmAI GTCTC 2 cut(s) 166, 948
BsmBI CGTCTC 2 cut(s) 166, 948
BsmFI GGGAC 2 cut(s) 28, 388
BsnI GGCC 3 cut(s) 425, 492, 632
BsoBI CYCGRG 1 cut(s) 104
Bsp1286I GDGCHC 3 cut(s) 111, 243, 550
Bsp143I GATC 3 cut(s) 87, 436, 733
Bsp19I CCATGG 2 cut(s) 426, 748
BspACI CCGC 2 cut(s) 132, 482
BspANI GGCC 3 cut(s) 425, 492, 632
BspCNI CTCAG 3 cut(s) 56, 439, 919
BspFNI CGCG 1 cut(s) 75
BspLI GGNNCC 1 cut(s) 201
BspMI ACCTGC 1 cut(s) 604
BspPI GGATC 1 cut(s) 444
BspTI CTTAAG 1 cut(s) 419
BsrI ACTGG 3 cut(s) 330, 775, 947
BssECI CCNNGG 5 cut(s) 121, 226, 227, 426, 748
BssMI GATC 3 cut(s) 87, 436, 733
BssNI GRCGYC 1 cut(s) 23
BssT1I CCWWGG 2 cut(s) 426, 748
Bst2UI CCWGG 3 cut(s) 228, 317, 634
Bst4CI ACNGT 4 cut(s) 19, 367, 970, 1040
Bst6I CTCTTC 1 cut(s) 37
BstACI GRCGYC 1 cut(s) 23
BstAFI CTTAAG 1 cut(s) 419
BstAPI GCANNNNNTGC 2 cut(s) 596, 718
BstC8I GCNNGC 2 cut(s) 77, 1056
BstDEI CTNAG 3 cut(s) 43, 447, 927
BstDSI CCRYGG 3 cut(s) 121, 426, 748
BstF5I GGATG 4 cut(s) 298, 642, 646, 703
BstFNI CGCG 1 cut(s) 75
BstHHI GCGC 1 cut(s) 75
BstKTI GATC 3 cut(s) 90, 439, 736
BstMAI GTCTC 2 cut(s) 166, 948
BstMBI GATC 3 cut(s) 87, 436, 733
BstMCI CGRYCG 1 cut(s) 90
BstMWI GCNNNNNNNGC 5 cut(s) 238, 587, 596, 709, 718
BstNI CCWGG 3 cut(s) 228, 317, 634
BstNSI RCATGY 1 cut(s) 1058
BstSCI CCNGG 3 cut(s) 226, 315, 632
BstSLI GKGCMC 2 cut(s) 243, 550
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 3 cut(s) 77, 227, 299
BstV2I GAAGAC 1 cut(s) 57
BstX2I RGATCY 1 cut(s) 436
BstXI CCANNNNNNTGG 1 cut(s) 122
BstYI RGATCY 1 cut(s) 436
BsuRI GGCC 3 cut(s) 425, 492, 632
BtgI CCRYGG 3 cut(s) 121, 426, 748
BtsCI GGATG 4 cut(s) 298, 642, 646, 703
BtsIMutI CAGTG 1 cut(s) 51
BveI ACCTGC 1 cut(s) 604
Cac8I GCNNGC 2 cut(s) 77, 1056
CfoI GCGC 1 cut(s) 75
Cfr13I GGNCC 2 cut(s) 351, 490
CseI GACGC 2 cut(s) 31, 930
Csp6I GTAC 1 cut(s) 772
CviAII CATG 5 cut(s) 427, 713, 749, 890, 1055
CviQI GTAC 1 cut(s) 772
DdeI CTNAG 3 cut(s) 43, 447, 927
DpnI GATC 3 cut(s) 89, 438, 735
DpnII GATC 3 cut(s) 87, 436, 733
DraIII CACNNNGTG 1 cut(s) 689
EaeI YGGCCR 1 cut(s) 630
Eam1104I CTCTTC 1 cut(s) 37
EarI CTCTTC 1 cut(s) 37
Ecl136II GAGCTC 1 cut(s) 109
Eco130I CCWWGG 2 cut(s) 426, 748
Eco24I GRGCYC 1 cut(s) 111
Eco47I GGWCC 1 cut(s) 351
Eco53kI GAGCTC 1 cut(s) 109
Eco88I CYCGRG 1 cut(s) 104
EcoICRI GAGCTC 1 cut(s) 109
EcoO109I RGGNCCY 2 cut(s) 351, 490
EcoRII CCWGG 3 cut(s) 226, 315, 632
EcoT14I CCWWGG 2 cut(s) 426, 748
EcoT38I GRGCYC 1 cut(s) 111
ErhI CCWWGG 2 cut(s) 426, 748
Esp3I CGTCTC 2 cut(s) 166, 948
FaeI CATG 5 cut(s) 430, 716, 752, 893, 1058
FaqI GGGAC 2 cut(s) 28, 388
FatI CATG 5 cut(s) 426, 712, 748, 889, 1054
FbaI TGATCA 1 cut(s) 733
Fnu4HI GCNGC 4 cut(s) 66, 132, 216, 313
FokI GGATG 4 cut(s) 305, 649, 653, 710
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 4 cut(s) 66, 132, 216, 313
GlaI GCGC 1 cut(s) 74
GluI GCNGC 4 cut(s) 66, 132, 216, 313
GsuI CTGGAG 3 cut(s) 597, 774, 930
HaeIII GGCC 3 cut(s) 425, 492, 632
HgaI GACGC 2 cut(s) 31, 930
HhaI GCGC 1 cut(s) 75
Hin1I GRCGYC 1 cut(s) 23
Hin1II CATG 5 cut(s) 430, 716, 752, 893, 1058
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HinfI GANTC 6 cut(s) 168, 401, 413, 560, 754, 863
HphI GGTGA 2 cut(s) 31, 271
Hpy166II GTNNAC 2 cut(s) 241, 548
Hpy188I TCNGA 8 cut(s) 478, 535, 678, 733, 738, 769, 874, 966
Hpy188III TCNNGA 6 cut(s) 165, 434, 576, 791, 860, 981
Hpy8I GTNNAC 2 cut(s) 241, 548
HpyAV CCTTC 2 cut(s) 205, 385
HpyCH4III ACNGT 4 cut(s) 19, 367, 970, 1040
HpyCH4IV ACGT 1 cut(s) 661
HpyCH4V TGCA 5 cut(s) 79, 241, 548, 590, 721
HpyF10VI GCNNNNNNNGC 5 cut(s) 238, 587, 596, 709, 718
HpyF3I CTNAG 3 cut(s) 43, 447, 927
HpySE526I ACGT 1 cut(s) 661
Hsp92I GRCGYC 1 cut(s) 23
Hsp92II CATG 5 cut(s) 430, 716, 752, 893, 1058
HspAI GCGC 1 cut(s) 73
Ksp22I TGATCA 1 cut(s) 733
Kzo9I GATC 3 cut(s) 87, 436, 733
LmnI GCTCC 2 cut(s) 479, 578
Lsp1109I GCAGC 3 cut(s) 77, 227, 299
LweI GCATC 2 cut(s) 283, 730
MaeII ACGT 1 cut(s) 661
MaeIII GTNAC 2 cut(s) 19, 335
MalI GATC 3 cut(s) 89, 438, 735
MboI GATC 3 cut(s) 87, 436, 733
MboII GAAGA 9 cut(s) 24, 31, 62, 521, 522, 617, 623, 668, 836
MfeI CAATTG 1 cut(s) 287
MflI RGATCY 1 cut(s) 436
MhlI GDGCHC 3 cut(s) 111, 243, 550
MlsI TGGCCA 1 cut(s) 632
MluCI AATT 7 cut(s) 287, 300, 571, 585, 614, 762, 1021
MluNI TGGCCA 1 cut(s) 632
MlyI GAGTC 2 cut(s) 162, 410
MmeI TCCRAC 1 cut(s) 378
Mox20I TGGCCA 1 cut(s) 632
MscI TGGCCA 1 cut(s) 632
MseI TTAA 2 cut(s) 153, 420
MslI CAYNNNNRTG 2 cut(s) 120, 246
Msp20I TGGCCA 1 cut(s) 632
MspCI CTTAAG 1 cut(s) 419
MspR9I CCNGG 3 cut(s) 228, 317, 634
MunI CAATTG 1 cut(s) 287
MvaI CCWGG 3 cut(s) 228, 317, 634
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 5 cut(s) 238, 587, 596, 709, 718
NcoI CCATGG 2 cut(s) 426, 748
NdeII GATC 3 cut(s) 87, 436, 733
NlaIII CATG 5 cut(s) 430, 716, 752, 893, 1058
NlaIV GGNNCC 1 cut(s) 201
NmuCI GTSAC 1 cut(s) 19
NspI RCATGY 1 cut(s) 1058
OliI CACNNNNGTG 1 cut(s) 120
PaeI GCATGC 1 cut(s) 1058
PaeR7I CTCGAG 1 cut(s) 104
PasI CCCWGGG 1 cut(s) 227
PfeI GAWTC 4 cut(s) 413, 560, 754, 863
PkrI GCNGC 4 cut(s) 67, 133, 217, 314
Ple19I CGATCG 1 cut(s) 90
PleI GAGTC 2 cut(s) 162, 409
PpsI GAGTC 2 cut(s) 162, 409
PpuMI RGGWCCY 1 cut(s) 351
Psp124BI GAGCTC 1 cut(s) 111
Psp5II RGGWCCY 1 cut(s) 351
Psp6I CCWGG 3 cut(s) 226, 315, 632
PspGI CCWGG 3 cut(s) 226, 315, 632
PspN4I GGNNCC 1 cut(s) 201
PspPI GGNCC 2 cut(s) 351, 490
PspPPI RGGWCCY 1 cut(s) 351
PspXI VCTCGAGB 1 cut(s) 104
PsuI RGATCY 1 cut(s) 436
PvuI CGATCG 1 cut(s) 90
RsaI GTAC 1 cut(s) 773
RsaNI GTAC 1 cut(s) 772
RseI CAYNNNNRTG 2 cut(s) 120, 246
SacI GAGCTC 1 cut(s) 111
SaqAI TTAA 2 cut(s) 153, 420
SatI GCNGC 4 cut(s) 66, 132, 216, 313
Sau3AI GATC 3 cut(s) 87, 436, 733
Sau96I GGNCC 2 cut(s) 351, 490
SchI GAGTC 2 cut(s) 162, 410
ScrFI CCNGG 3 cut(s) 228, 317, 634
SduI GDGCHC 3 cut(s) 111, 243, 550
SfaNI GCATC 2 cut(s) 283, 730
Sfr274I CTCGAG 1 cut(s) 104
SinI GGWCC 1 cut(s) 351
SlaI CTCGAG 1 cut(s) 104
SmiMI CAYNNNNRTG 2 cut(s) 120, 246
SmlI CTYRAG 4 cut(s) 104, 419, 551, 647
SmoI CTYRAG 4 cut(s) 104, 419, 551, 647
SphI GCATGC 1 cut(s) 1058
Sse9I AATT 7 cut(s) 287, 300, 571, 585, 614, 762, 1021
SsiI CCGC 2 cut(s) 132, 482
SstI GAGCTC 1 cut(s) 111
StyD4I CCNGG 3 cut(s) 226, 315, 632
StyI CCWWGG 2 cut(s) 426, 748
TaaI ACNGT 4 cut(s) 19, 367, 970, 1040
TaiI ACGT 1 cut(s) 664
TaqI TCGA 4 cut(s) 105, 111, 356, 411
TasI AATT 7 cut(s) 287, 300, 571, 585, 614, 762, 1021
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 4 cut(s) 413, 560, 754, 863
Tru1I TTAA 2 cut(s) 153, 420
Tru9I TTAA 2 cut(s) 153, 420
TscAI CASTG 1 cut(s) 51
TseFI GTSAC 1 cut(s) 19
TseI GCWGC 3 cut(s) 65, 215, 312
Tsp45I GTSAC 1 cut(s) 19
TspDTI ATGAA 6 cut(s) 17, 618, 624, 657, 837, 906
TspGWI ACGGA 1 cut(s) 110
TspRI CASTG 1 cut(s) 51
Vha464I CTTAAG 1 cut(s) 419
VneI GTGCAC 2 cut(s) 239, 546
VpaK11BI GGWCC 1 cut(s) 351
XapI RAATTY 2 cut(s) 614, 762
XceI RCATGY 1 cut(s) 1058
XcmI CCANNNNNNNNNTGG 2 cut(s) 43, 253
XhoI CTCGAG 1 cut(s) 104
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.