RLG00000033455

RNA polymerase II subunit 5-mediating protein homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002516
Physical Location & Seq
Forward (+)
27454887 .. 27461451
6565 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033455

Sequence Viewer

Length: 1107 bp
ATGAAAGGCAAGGGGACGGTGACGCCTCTCTCCTCCGTCTTCTCAGTGGAAGACACACAGAAAGCAGTTCAGCGCGTGCAGGACACGATCGCCCAGAAGGACGTCGAGCTCGACCACCTCCGTGGCTTCGCTGCTGACAACACAGACCTCATTAACCTTGTCTCCCGACTCCCTGAACACCTCCACCACGACATTATGGTTCCATTTGGGAAGGCAGCATTTTTCCCAGGGCGTTTGGTGCACACCAATGAGTTTATGGTTCTACTGGGAGAAAGGTACTATGCCGAAAGGACCTCGAAGCAAACAGTAGATATTTTGAACAGGAGAGGGAAGGTATTGGAGTCCCAAGTCGATTCACTTAAGGCCATGGTTCAGGATCTCAAAGCTGAGGCTTCCTTTTTCAATGCTACTGCCTCCGAAGCAGCAGAGGGCCTTGTAGAGATAATGGAAGATTATGAAGAGGAAAGTTCTTCTGATAGAGAATGTGCACTCAAGCAAGATTCCTTTAATAATTCTGGAGCTGTAATTGCAAAACCTGCTGATGTAGATGAAGAATTTGCTCATATGATGGCCAGGATGGATGAACTTGAGAAAGAAGAACGTGAAGCTGAAAGTCAGATGGCACAAAGTGATGAGGATGAGCAAGTTCAGCATGGTTTGCATCAGCTTTCTGATCAGAAATCTCTCCATGGGAATCTCAAAATTTCGGAGTACCAGTCAAGGAAACCTCCAGAGCAGACAAAAGATGGAGTTACTTCTAATGAAGATAGTTTTACTGATGTGCCAAGTTGTTCTGGTCTGGAATCTATATCTGACGATAAAGTATCACATGAAAATATATTGGGCCATGATGTAAAAAGTGTTGCTGAGAAAGATTTGGCATCTCCAGTAGTTGAGAATGTTCAGACTGTGCCTTTATCAAGAAGTCAGGTTTCTCTTGAAAGTTCAAAACCAAAATCTGACCGGGAGAAAGCTTTTACAGGTTCTATTGTTGAGCACACTGATAATCTTCAGATAAAATCAAGGGAAAGAACTACGGCTACATCACAGTCCTCTGCTTCTCAAGTTTCAAAGCCGGTTTCAAGATTCAAGATGCAGAGAAAGTAG

Protein Analysis

369

Amino Acids

40.96

Weight (kDa)

5.04

Isoelectric Point (pI)

41.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Prefoldin PF02996 27 - 142 9.6e-20 Prefoldin subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 105
Acc36I ACCTGC 1 cut(s) 544
AccII CGCG 1 cut(s) 75
AclWI GGATC 1 cut(s) 384
AcoI YGGCCR 1 cut(s) 570
AcsI RAATTY 2 cut(s) 554, 702
AcuI CTGAAG 1 cut(s) 995
AcyI GRCGYC 2 cut(s) 23, 102
AdeI CACNNNGTG 1 cut(s) 629
AfaI GTAC 2 cut(s) 278, 713
AflII CTTAAG 1 cut(s) 359
AgsI TTSAA 7 cut(s) 319, 403, 941, 948, 1071, 1083, 1090
AjnI CCWGG 2 cut(s) 226, 572
AleI CACNNNNGTG 1 cut(s) 120
AluBI AGCT 6 cut(s) 109, 386, 521, 608, 667, 974
AluI AGCT 6 cut(s) 109, 386, 521, 608, 667, 974
Alw21I GWGCWC 4 cut(s) 111, 243, 490, 999
Alw26I GTCTC 1 cut(s) 166
Alw44I GTGCAC 2 cut(s) 239, 486
AlwI GGATC 1 cut(s) 384
AoxI GGCC 4 cut(s) 363, 430, 570, 844
ApaLI GTGCAC 2 cut(s) 239, 486
ApeKI GCWGC 3 cut(s) 131, 215, 422
ApoI RAATTY 2 cut(s) 554, 702
AspLEI GCGC 1 cut(s) 75
AspS9I GGNCC 3 cut(s) 291, 430, 844
AsuC2I CCSGG 1 cut(s) 965
AsuHPI GGTGA 1 cut(s) 31
AvaII GGWCC 1 cut(s) 291
BaeGI GKGCMC 2 cut(s) 243, 490
BalI TGGCCA 1 cut(s) 572
BanII GRGCYC 1 cut(s) 111
BbsI GAAGAC 2 cut(s) 31, 57
Bbv12I GWGCWC 4 cut(s) 111, 243, 490, 999
BbvCI CCTCAGC 1 cut(s) 387
BbvI GCAGC 3 cut(s) 118, 227, 434
BccI CCATC 4 cut(s) 562, 571, 613, 740
BceAI ACGGC 1 cut(s) 1053
BciT130I CCWGG 2 cut(s) 228, 574
BclI TGATCA 1 cut(s) 673
BcnI CCSGG 1 cut(s) 965
BcoDI GTCTC 1 cut(s) 166
BfrI CTTAAG 1 cut(s) 359
BfuAI ACCTGC 1 cut(s) 544
BisI GCNGC 3 cut(s) 132, 216, 423
BlsI GCNGC 3 cut(s) 133, 217, 424
Bme1390I CCNGG 3 cut(s) 228, 574, 965
Bme18I GGWCC 1 cut(s) 291
BmgT120I GGNCC 3 cut(s) 291, 430, 844
BmiI GGNNCC 1 cut(s) 201
BmrFI CCNGG 3 cut(s) 228, 574, 965
BmrI ACTGGG 1 cut(s) 275
BmsI GCATC 3 cut(s) 670, 890, 1083
BmuI ACTGGG 1 cut(s) 275
BpiI GAAGAC 2 cut(s) 31, 57
BpmI CTGGAG 3 cut(s) 537, 714, 870
Bpu10I CCTNAGC 1 cut(s) 387
BpuEI CTTGAG 3 cut(s) 476, 608, 1047
BpuMI CCSGG 1 cut(s) 965
BsaHI GRCGYC 2 cut(s) 23, 102
BsaJI CCNNGG 5 cut(s) 121, 226, 227, 366, 688
BsaXI ACNNNNNCTCC 4 cut(s) 146, 176, 701, 731
Bse118I RCCGGY 1 cut(s) 1075
Bse1I ACTGG 3 cut(s) 270, 715, 887
BseBI CCWGG 2 cut(s) 228, 574
BseDI CCNNGG 5 cut(s) 121, 226, 227, 366, 688
BseGI GGATG 3 cut(s) 582, 586, 643
BseMII CTCAG 3 cut(s) 57, 378, 858
BseNI ACTGG 3 cut(s) 270, 715, 887
BseRI GAGGAG 1 cut(s) 22
BseSI GKGCMC 2 cut(s) 243, 490
BseXI GCAGC 3 cut(s) 118, 227, 434
BsgI GTGCAG 1 cut(s) 98
Bsh1236I CGCG 1 cut(s) 75
Bsh1285I CGRYCG 1 cut(s) 90
BshFI GGCC 4 cut(s) 365, 432, 572, 846
BsiEI CGRYCG 1 cut(s) 90
BsiHKAI GWGCWC 4 cut(s) 111, 243, 490, 999
BsiSI CCGG 2 cut(s) 964, 1076
BslFI GGGAC 2 cut(s) 28, 328
BsmAI GTCTC 1 cut(s) 166
BsmFI GGGAC 2 cut(s) 28, 328
BsnI GGCC 4 cut(s) 365, 432, 572, 846
Bsp1286I GDGCHC 4 cut(s) 111, 243, 490, 999
Bsp143I GATC 3 cut(s) 87, 376, 673
Bsp19I CCATGG 2 cut(s) 366, 688
BspANI GGCC 4 cut(s) 365, 432, 572, 846
BspCNI CTCAG 3 cut(s) 56, 379, 859
BspFNI CGCG 1 cut(s) 75
BspLI GGNNCC 1 cut(s) 201
BspMI ACCTGC 1 cut(s) 544
BspPI GGATC 1 cut(s) 384
BspTI CTTAAG 1 cut(s) 359
BsrFI RCCGGY 1 cut(s) 1075
BsrI ACTGG 3 cut(s) 270, 715, 887
BssAI RCCGGY 1 cut(s) 1075
BssECI CCNNGG 5 cut(s) 121, 226, 227, 366, 688
BssMI GATC 3 cut(s) 87, 376, 673
BssNI GRCGYC 2 cut(s) 23, 102
BssT1I CCWWGG 2 cut(s) 366, 688
Bst2UI CCWGG 2 cut(s) 228, 574
Bst4CI ACNGT 4 cut(s) 19, 307, 910, 1050
Bst6I CTCTTC 1 cut(s) 453
BstACI GRCGYC 2 cut(s) 23, 102
BstAFI CTTAAG 1 cut(s) 359
BstAPI GCANNNNNTGC 2 cut(s) 536, 658
BstC8I GCNNGC 1 cut(s) 77
BstDEI CTNAG 3 cut(s) 43, 387, 867
BstDSI CCRYGG 3 cut(s) 121, 366, 688
BstF5I GGATG 3 cut(s) 582, 586, 643
BstFNI CGCG 1 cut(s) 75
BstHHI GCGC 1 cut(s) 75
BstKTI GATC 3 cut(s) 90, 379, 676
BstMAI GTCTC 1 cut(s) 166
BstMBI GATC 3 cut(s) 87, 376, 673
BstMCI CGRYCG 1 cut(s) 90
BstMWI GCNNNNNNNGC 6 cut(s) 238, 419, 527, 536, 649, 658
BstNI CCWGG 2 cut(s) 228, 574
BstSCI CCNGG 3 cut(s) 226, 572, 963
BstSLI GKGCMC 2 cut(s) 243, 490
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 3 cut(s) 118, 227, 434
BstV2I GAAGAC 2 cut(s) 31, 57
BstX2I RGATCY 1 cut(s) 376
BstXI CCANNNNNNTGG 1 cut(s) 122
BstYI RGATCY 1 cut(s) 376
BsuRI GGCC 4 cut(s) 365, 432, 572, 846
BtgI CCRYGG 3 cut(s) 121, 366, 688
BtsCI GGATG 3 cut(s) 582, 586, 643
BtsIMutI CAGTG 2 cut(s) 51, 999
BveI ACCTGC 1 cut(s) 544
Cac8I GCNNGC 1 cut(s) 77
CfoI GCGC 1 cut(s) 75
Cfr10I RCCGGY 1 cut(s) 1075
Cfr13I GGNCC 3 cut(s) 291, 430, 844
CseI GACGC 1 cut(s) 31
Csp6I GTAC 2 cut(s) 277, 712
CviAII CATG 5 cut(s) 367, 653, 689, 830, 848
CviQI GTAC 2 cut(s) 277, 712
DdeI CTNAG 3 cut(s) 43, 387, 867
DpnI GATC 3 cut(s) 89, 378, 675
DpnII GATC 3 cut(s) 87, 376, 673
DraIII CACNNNGTG 1 cut(s) 629
EaeI YGGCCR 1 cut(s) 570
Eam1104I CTCTTC 1 cut(s) 453
EarI CTCTTC 1 cut(s) 453
Ecl136II GAGCTC 1 cut(s) 109
Eco130I CCWWGG 2 cut(s) 366, 688
Eco24I GRGCYC 1 cut(s) 111
Eco47I GGWCC 1 cut(s) 291
Eco53kI GAGCTC 1 cut(s) 109
Eco57I CTGAAG 1 cut(s) 995
EcoICRI GAGCTC 1 cut(s) 109
EcoO109I RGGNCCY 2 cut(s) 291, 430
EcoRII CCWGG 2 cut(s) 226, 572
EcoT14I CCWWGG 2 cut(s) 366, 688
EcoT38I GRGCYC 1 cut(s) 111
ErhI CCWWGG 2 cut(s) 366, 688
FaeI CATG 5 cut(s) 370, 656, 692, 833, 851
FaqI GGGAC 2 cut(s) 28, 328
FatI CATG 5 cut(s) 366, 652, 688, 829, 847
FauNDI CATATG 1 cut(s) 564
FbaI TGATCA 1 cut(s) 673
Fnu4HI GCNGC 3 cut(s) 132, 216, 423
FokI GGATG 3 cut(s) 589, 593, 650
FriOI GRGCYC 1 cut(s) 111
Fsp4HI GCNGC 3 cut(s) 132, 216, 423
GlaI GCGC 1 cut(s) 74
GluI GCNGC 3 cut(s) 132, 216, 423
GsuI CTGGAG 3 cut(s) 537, 714, 870
HaeIII GGCC 4 cut(s) 365, 432, 572, 846
HapII CCGG 2 cut(s) 964, 1076
HgaI GACGC 1 cut(s) 31
HhaI GCGC 1 cut(s) 75
Hin1I GRCGYC 2 cut(s) 23, 102
Hin1II CATG 5 cut(s) 370, 656, 692, 833, 851
Hin6I GCGC 1 cut(s) 73
HinP1I GCGC 1 cut(s) 73
HindIII AAGCTT 1 cut(s) 972
HinfI GANTC 7 cut(s) 168, 341, 353, 500, 694, 803, 1086
HpaII CCGG 2 cut(s) 964, 1076
HphI GGTGA 1 cut(s) 31
Hpy166II GTNNAC 2 cut(s) 241, 488
Hpy188III TCNNGA 9 cut(s) 165, 374, 516, 731, 800, 921, 938, 1083, 1090
Hpy8I GTNNAC 2 cut(s) 241, 488
Hpy99I CGWCG 1 cut(s) 107
HpyAV CCTTC 3 cut(s) 91, 205, 325
HpyCH4III ACNGT 4 cut(s) 19, 307, 910, 1050
HpyCH4IV ACGT 2 cut(s) 102, 601
HpyCH4V TGCA 6 cut(s) 79, 241, 488, 530, 661, 1096
HpyF10VI GCNNNNNNNGC 6 cut(s) 238, 419, 527, 536, 649, 658
HpyF3I CTNAG 3 cut(s) 43, 387, 867
HpySE526I ACGT 2 cut(s) 102, 601
Hsp92I GRCGYC 2 cut(s) 23, 102
Hsp92II CATG 5 cut(s) 370, 656, 692, 833, 851
HspAI GCGC 1 cut(s) 73
Ksp22I TGATCA 1 cut(s) 673
Kzo9I GATC 3 cut(s) 87, 376, 673
LmnI GCTCC 1 cut(s) 518
Lsp1109I GCAGC 3 cut(s) 118, 227, 434
LweI GCATC 3 cut(s) 670, 890, 1083
MaeII ACGT 2 cut(s) 102, 601
MaeIII GTNAC 2 cut(s) 19, 751
MalI GATC 3 cut(s) 89, 378, 675
MboI GATC 3 cut(s) 87, 376, 673
MboII GAAGA 9 cut(s) 31, 62, 461, 462, 470, 563, 608, 776, 1001
MflI RGATCY 1 cut(s) 376
MhlI GDGCHC 4 cut(s) 111, 243, 490, 999
MlsI TGGCCA 1 cut(s) 572
MluCI AATT 4 cut(s) 511, 525, 554, 702
MluNI TGGCCA 1 cut(s) 572
MlyI GAGTC 2 cut(s) 162, 350
Mox20I TGGCCA 1 cut(s) 572
MscI TGGCCA 1 cut(s) 572
MseI TTAA 3 cut(s) 153, 360, 507
MslI CAYNNNNRTG 2 cut(s) 120, 246
Msp20I TGGCCA 1 cut(s) 572
MspCI CTTAAG 1 cut(s) 359
MspI CCGG 2 cut(s) 964, 1076
MspR9I CCNGG 3 cut(s) 228, 574, 965
MvaI CCWGG 2 cut(s) 228, 574
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 6 cut(s) 238, 419, 527, 536, 649, 658
NciI CCSGG 1 cut(s) 965
NcoI CCATGG 2 cut(s) 366, 688
NdeI CATATG 1 cut(s) 564
NdeII GATC 3 cut(s) 87, 376, 673
NlaIII CATG 5 cut(s) 370, 656, 692, 833, 851
NlaIV GGNNCC 1 cut(s) 201
NmuCI GTSAC 1 cut(s) 19
OliI CACNNNNGTG 1 cut(s) 120
PasI CCCWGGG 1 cut(s) 227
PcsI WCGNNNNNNNCGW 1 cut(s) 108
PfeI GAWTC 5 cut(s) 353, 500, 694, 803, 1086
PkrI GCNGC 3 cut(s) 133, 217, 424
Ple19I CGATCG 1 cut(s) 90
PleI GAGTC 2 cut(s) 162, 349
PpsI GAGTC 2 cut(s) 162, 349
PpuMI RGGWCCY 1 cut(s) 291
Psp124BI GAGCTC 1 cut(s) 111
Psp5II RGGWCCY 1 cut(s) 291
Psp6I CCWGG 2 cut(s) 226, 572
PspGI CCWGG 2 cut(s) 226, 572
PspN4I GGNNCC 1 cut(s) 201
PspPI GGNCC 3 cut(s) 291, 430, 844
PspPPI RGGWCCY 1 cut(s) 291
PsrI GAACNNNNNNTAC 2 cut(s) 1024, 1056
PsuI RGATCY 1 cut(s) 376
PvuI CGATCG 1 cut(s) 90
RsaI GTAC 2 cut(s) 278, 713
RsaNI GTAC 2 cut(s) 277, 712
RseI CAYNNNNRTG 2 cut(s) 120, 246
SacI GAGCTC 1 cut(s) 111
SaqAI TTAA 3 cut(s) 153, 360, 507
SatI GCNGC 3 cut(s) 132, 216, 423
Sau3AI GATC 3 cut(s) 87, 376, 673
Sau96I GGNCC 3 cut(s) 291, 430, 844
SchI GAGTC 2 cut(s) 162, 350
ScrFI CCNGG 3 cut(s) 228, 574, 965
SduI GDGCHC 4 cut(s) 111, 243, 490, 999
SfaNI GCATC 3 cut(s) 670, 890, 1083
SinI GGWCC 1 cut(s) 291
SmiMI CAYNNNNRTG 2 cut(s) 120, 246
SmlI CTYRAG 4 cut(s) 359, 491, 587, 1062
SmoI CTYRAG 4 cut(s) 359, 491, 587, 1062
Sse9I AATT 4 cut(s) 511, 525, 554, 702
SstI GAGCTC 1 cut(s) 111
StyD4I CCNGG 3 cut(s) 226, 572, 963
StyI CCWWGG 2 cut(s) 366, 688
TaaI ACNGT 4 cut(s) 19, 307, 910, 1050
TaiI ACGT 2 cut(s) 105, 604
TaqI TCGA 4 cut(s) 105, 111, 296, 351
TasI AATT 4 cut(s) 511, 525, 554, 702
TfiI GAWTC 5 cut(s) 353, 500, 694, 803, 1086
Tru1I TTAA 3 cut(s) 153, 360, 507
Tru9I TTAA 3 cut(s) 153, 360, 507
TscAI CASTG 2 cut(s) 51, 1006
TseFI GTSAC 1 cut(s) 19
TseI GCWGC 3 cut(s) 131, 215, 422
Tsp45I GTSAC 1 cut(s) 19
TspDTI ATGAA 6 cut(s) 17, 471, 564, 597, 777, 846
TspGWI ACGGA 2 cut(s) 25, 110
TspRI CASTG 2 cut(s) 51, 1006
Vha464I CTTAAG 1 cut(s) 359
VneI GTGCAC 2 cut(s) 239, 486
VpaK11BI GGWCC 1 cut(s) 291
XapI RAATTY 2 cut(s) 554, 702
XcmI CCANNNNNNNNNTGG 1 cut(s) 253
ZraI GACGTC 1 cut(s) 103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.