RLG00000000647

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0014842
Physical Location & Seq
Reverse (-)
3085609 .. 3086150
542 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000647

Sequence Viewer

Length: 450 bp
ATGAGAGTCGAGAGGGTGGAGAGCCTTTCCTCGGCGAACCCTAGTGCCGCTCCCTTGGTGCGGCTTCCAGGGAGGACTCTGCTGCCTCCACTTAATCTACGTTTGAGGCTGACTTTTGATCAGCTCTTTCCTCGTGTCGGGGAGAGGCGGATTACAGGCTGTTTGGATTGGCTCAGTTTCCGTCGGCGACCATGGCGGGGACAACTCCCAGGCTGGGTGAGGAGACCCGAGATCGGGCTGGAGCGGCGTCTTCCTTCTTTTAGGGATTGGAAGATTTTCGATGGAGGCACAGGAATCAGAATCTTGCTGTGGTGGAAGTGCGATGCGGTACCCACGAACTCTATGGAAGATCTAGATTTGGGACCCAGAGTCGATGGCTTGGCTGATCGGATCCTTCATCCAGGGAGGCTGGCGGCTTTGTTGGGTGCAGACGAAGCTTTGGGTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

16.96

Weight (kDa)

10.8

Isoelectric Point (pI)

53.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 328
AccB1I GGYRCC 1 cut(s) 328
AccBSI CCGCTC 2 cut(s) 50, 244
AciI CCGC 7 cut(s) 48, 61, 148, 196, 244, 326, 413
AclWI GGATC 2 cut(s) 385, 398
AcyI GRCGYC 1 cut(s) 247
AfaI GTAC 1 cut(s) 330
AfiI CCNNNNNNNGG 7 cut(s) 31, 60, 137, 197, 214, 233, 234
AhdI GACNNNNNGTC 1 cut(s) 368
AjnI CCWGG 3 cut(s) 67, 208, 400
AluBI AGCT 2 cut(s) 124, 437
AluI AGCT 2 cut(s) 124, 437
Alw26I GTCTC 1 cut(s) 217
AlwI GGATC 2 cut(s) 385, 398
Ama87I CYCGRG 1 cut(s) 227
ApeKI GCWGC 1 cut(s) 82
Asp700I GAANNNNTTC 1 cut(s) 275
Asp718I GGTACC 1 cut(s) 328
AspS9I GGNCC 1 cut(s) 362
AsuHPI GGTGA 1 cut(s) 229
AvaI CYCGRG 1 cut(s) 227
AvaII GGWCC 1 cut(s) 362
BamHI GGATCC 1 cut(s) 390
BanI GGYRCC 1 cut(s) 328
BauI CACGAG 1 cut(s) 132
BbsI GAAGAC 1 cut(s) 242
BbvI GCAGC 1 cut(s) 69
BccI CCATC 2 cut(s) 275, 368
BciT130I CCWGG 3 cut(s) 69, 210, 402
BclI TGATCA 1 cut(s) 118
BcoDI GTCTC 1 cut(s) 217
BfaI CTAG 2 cut(s) 42, 353
BglII AGATCT 1 cut(s) 349
BisI GCNGC 5 cut(s) 48, 62, 83, 245, 414
BlsI GCNGC 5 cut(s) 49, 63, 84, 246, 415
Bme1390I CCNGG 3 cut(s) 69, 210, 402
Bme18I GGWCC 1 cut(s) 362
BmeRI GACNNNNNGTC 1 cut(s) 368
BmeT110I CYCGRG 1 cut(s) 227
BmgT120I GGNCC 1 cut(s) 362
BmiI GGNNCC 4 cut(s) 330, 363, 364, 392
BmrFI CCNGG 3 cut(s) 69, 210, 402
BmsI GCATC 1 cut(s) 313
BpiI GAAGAC 1 cut(s) 242
BpmI CTGGAG 1 cut(s) 260
BsaHI GRCGYC 1 cut(s) 247
BsaI GGTCTC 1 cut(s) 217
BsaJI CCNNGG 6 cut(s) 30, 54, 68, 191, 208, 401
Bsc4I CCNNNNNNNGG 7 cut(s) 31, 60, 137, 197, 214, 233, 234
BseBI CCWGG 3 cut(s) 69, 210, 402
BseDI CCNNGG 6 cut(s) 30, 54, 68, 191, 208, 401
BseGI GGATG 1 cut(s) 397
BseLI CCNNNNNNNGG 7 cut(s) 31, 60, 137, 197, 214, 233, 234
BseMII CTCAG 1 cut(s) 187
BseRI GAGGAG 1 cut(s) 235
BseXI GCAGC 1 cut(s) 69
BseYI CCCAGC 1 cut(s) 213
BsgI GTGCAG 1 cut(s) 447
BshNI GGYRCC 1 cut(s) 328
BsiHKCI CYCGRG 1 cut(s) 227
BslFI GGGAC 2 cut(s) 213, 375
BslI CCNNNNNNNGG 7 cut(s) 31, 60, 137, 197, 214, 233, 234
BsmAI GTCTC 1 cut(s) 217
BsmFI GGGAC 2 cut(s) 213, 375
Bso31I GGTCTC 1 cut(s) 217
BsoBI CYCGRG 1 cut(s) 227
Bsp143I GATC 5 cut(s) 118, 231, 349, 385, 390
Bsp19I CCATGG 1 cut(s) 191
BspACI CCGC 7 cut(s) 48, 61, 148, 196, 244, 326, 413
BspCNI CTCAG 1 cut(s) 186
BspLI GGNNCC 4 cut(s) 330, 363, 364, 392
BspPI GGATC 2 cut(s) 385, 398
BspT107I GGYRCC 1 cut(s) 328
BspTNI GGTCTC 1 cut(s) 217
BsrBI CCGCTC 2 cut(s) 50, 244
BssECI CCNNGG 6 cut(s) 30, 54, 68, 191, 208, 401
BssMI GATC 5 cut(s) 118, 231, 349, 385, 390
BssNI GRCGYC 1 cut(s) 247
BssSI CACGAG 1 cut(s) 132
BssT1I CCWWGG 2 cut(s) 54, 191
Bst2BI CACGAG 1 cut(s) 132
Bst2UI CCWGG 3 cut(s) 69, 210, 402
BstACI GRCGYC 1 cut(s) 247
BstC8I GCNNGC 1 cut(s) 411
BstDEI CTNAG 1 cut(s) 173
BstDSI CCRYGG 1 cut(s) 191
BstF5I GGATG 1 cut(s) 397
BstKTI GATC 5 cut(s) 121, 234, 352, 388, 393
BstMAI GTCTC 1 cut(s) 217
BstMBI GATC 5 cut(s) 118, 231, 349, 385, 390
BstMWI GCNNNNNNNGC 3 cut(s) 193, 244, 434
BstNI CCWGG 3 cut(s) 69, 210, 402
BstSCI CCNGG 3 cut(s) 67, 208, 400
BstV1I GCAGC 1 cut(s) 69
BstV2I GAAGAC 1 cut(s) 242
BstX2I RGATCY 2 cut(s) 349, 390
BstYI RGATCY 2 cut(s) 349, 390
BtgI CCRYGG 1 cut(s) 191
BtgZI GCGATG 1 cut(s) 336
BtsCI GGATG 1 cut(s) 397
Cac8I GCNNGC 1 cut(s) 411
Cfr13I GGNCC 1 cut(s) 362
CseI GACGC 1 cut(s) 236
Csp6I GTAC 1 cut(s) 329
CviAII CATG 1 cut(s) 192
CviQI GTAC 1 cut(s) 329
DdeI CTNAG 1 cut(s) 173
DpnI GATC 5 cut(s) 120, 233, 351, 387, 392
DpnII GATC 5 cut(s) 118, 231, 349, 385, 390
DriI GACNNNNNGTC 1 cut(s) 368
Eam1105I GACNNNNNGTC 1 cut(s) 368
EciI GGCGGA 1 cut(s) 163
Eco130I CCWWGG 2 cut(s) 54, 191
Eco31I GGTCTC 1 cut(s) 217
Eco47I GGWCC 1 cut(s) 362
Eco88I CYCGRG 1 cut(s) 227
EcoO109I RGGNCCY 1 cut(s) 362
EcoRII CCWGG 3 cut(s) 67, 208, 400
EcoT14I CCWWGG 2 cut(s) 54, 191
ErhI CCWWGG 2 cut(s) 54, 191
FaeI CATG 1 cut(s) 195
FaiI YATR 2 cut(s) 193, 344
FaqI GGGAC 2 cut(s) 213, 375
FatI CATG 1 cut(s) 191
FauI CCCGC 1 cut(s) 189
FbaI TGATCA 1 cut(s) 118
Fnu4HI GCNGC 5 cut(s) 48, 62, 83, 245, 414
FokI GGATG 1 cut(s) 384
Fsp4HI GCNGC 5 cut(s) 48, 62, 83, 245, 414
FspBI CTAG 2 cut(s) 42, 353
GluI GCNGC 5 cut(s) 48, 62, 83, 245, 414
GsaI CCCAGC 1 cut(s) 217
GsuI CTGGAG 1 cut(s) 260
HgaI GACGC 1 cut(s) 236
Hin1I GRCGYC 1 cut(s) 247
Hin1II CATG 1 cut(s) 195
HindIII AAGCTT 1 cut(s) 435
HinfI GANTC 5 cut(s) 6, 76, 294, 300, 369
HphI GGTGA 1 cut(s) 229
Hpy188I TCNGA 2 cut(s) 299, 390
Hpy188III TCNNGA 2 cut(s) 10, 353
Hpy99I CGWCG 1 cut(s) 186
HpyAV CCTTC 2 cut(s) 264, 404
HpyCH4IV ACGT 1 cut(s) 100
HpyCH4V TGCA 1 cut(s) 428
HpyF10VI GCNNNNNNNGC 3 cut(s) 193, 244, 434
HpyF3I CTNAG 1 cut(s) 173
HpySE526I ACGT 1 cut(s) 100
Hsp92I GRCGYC 1 cut(s) 247
Hsp92II CATG 1 cut(s) 195
KflI GGGWCCC 1 cut(s) 362
KpnI GGTACC 1 cut(s) 332
Ksp22I TGATCA 1 cut(s) 118
Kzo9I GATC 5 cut(s) 118, 231, 349, 385, 390
LmnI GCTCC 2 cut(s) 55, 241
Lsp1109I GCAGC 1 cut(s) 69
LweI GCATC 1 cut(s) 313
MaeI CTAG 2 cut(s) 42, 353
MaeII ACGT 1 cut(s) 100
MaeIII GTNAC 1 cut(s) 443
MalI GATC 5 cut(s) 120, 233, 351, 387, 392
MbiI CCGCTC 2 cut(s) 50, 244
MboI GATC 5 cut(s) 118, 231, 349, 385, 390
MboII GAAGA 3 cut(s) 242, 283, 359
MflI RGATCY 2 cut(s) 349, 390
MlyI GAGTC 3 cut(s) 15, 70, 378
MroXI GAANNNNTTC 1 cut(s) 275
MseI TTAA 1 cut(s) 93
MspR9I CCNGG 3 cut(s) 69, 210, 402
MvaI CCWGG 3 cut(s) 69, 210, 402
MwoI GCNNNNNNNGC 3 cut(s) 193, 244, 434
NcoI CCATGG 1 cut(s) 191
NdeII GATC 5 cut(s) 118, 231, 349, 385, 390
NlaIII CATG 1 cut(s) 195
NlaIV GGNNCC 4 cut(s) 330, 363, 364, 392
NmeAIII GCCGAG 1 cut(s) 11
NmuCI GTSAC 1 cut(s) 443
PdmI GAANNNNTTC 1 cut(s) 275
PfeI GAWTC 2 cut(s) 294, 300
PkrI GCNGC 5 cut(s) 49, 63, 84, 246, 415
PleI GAGTC 3 cut(s) 14, 70, 377
PpsI GAGTC 3 cut(s) 14, 70, 377
PpuMI RGGWCCY 1 cut(s) 362
Psp5II RGGWCCY 1 cut(s) 362
Psp6I CCWGG 3 cut(s) 67, 208, 400
PspFI CCCAGC 1 cut(s) 213
PspGI CCWGG 3 cut(s) 67, 208, 400
PspN4I GGNNCC 4 cut(s) 330, 363, 364, 392
PspPI GGNCC 1 cut(s) 362
PspPPI RGGWCCY 1 cut(s) 362
PsuI RGATCY 2 cut(s) 349, 390
RsaI GTAC 1 cut(s) 330
RsaNI GTAC 1 cut(s) 329
SaqAI TTAA 1 cut(s) 93
SatI GCNGC 5 cut(s) 48, 62, 83, 245, 414
Sau3AI GATC 5 cut(s) 118, 231, 349, 385, 390
Sau96I GGNCC 1 cut(s) 362
SchI GAGTC 3 cut(s) 15, 70, 378
ScrFI CCNGG 3 cut(s) 69, 210, 402
SetI ASST 3 cut(s) 103, 126, 439
SfaNI GCATC 1 cut(s) 313
SinI GGWCC 1 cut(s) 362
SsiI CCGC 7 cut(s) 48, 61, 148, 196, 244, 326, 413
SspMI CTAG 2 cut(s) 42, 353
StyD4I CCNGG 3 cut(s) 67, 208, 400
StyI CCWWGG 2 cut(s) 54, 191
TaiI ACGT 1 cut(s) 103
TaqI TCGA 3 cut(s) 9, 279, 372
TauI GCSGC 4 cut(s) 50, 64, 247, 416
TfiI GAWTC 2 cut(s) 294, 300
Tru1I TTAA 1 cut(s) 93
Tru9I TTAA 1 cut(s) 93
TseFI GTSAC 1 cut(s) 443
TseI GCWGC 1 cut(s) 82
Tsp45I GTSAC 1 cut(s) 443
TspDTI ATGAA 1 cut(s) 386
TspGWI ACGGA 1 cut(s) 170
VpaK11BI GGWCC 1 cut(s) 362
XbaI TCTAGA 1 cut(s) 352
XcmI CCANNNNNNNNNTGG 1 cut(s) 340
XmnI GAANNNNTTC 1 cut(s) 275
XspI CTAG 2 cut(s) 42, 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.