RLG00000013232

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0014842
Physical Location & Seq
Forward (+)
28057911 .. 28058882
972 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013232

Sequence Viewer

Length: 522 bp
ATGGTCGCTGCTGCACTCTTCTCTGCTCAAACCCTAGAGCAACGCTACGCCATGAGATTGAAAGCCATGATCCCACGATGTGGCGCTGGTGCAGAGCGGCGGCTGGGTCTTGATCTGGTGATTGGGATTAATGCTGGTGGCGTCGTTGAAGTGCCCAGCAATGGTGGCCAACGATGGTGGTTCGGAGTCAATAACCGCAGAGTCATTGCAGAATGGAGCCGGAGCCTTGCTTTGGTGGAAGGTCATGATCTTGTTGTCGTTGTGGGTGTAGATCGTTGGCGACGATGGGATTGGTCAAGAATTGAGTCTGGATGCCTTGTTCTTGGGTGTCCAGATCAGTGTGATGGAATACTTACGAGATCTATGGAAGATCTGGGTTTGGGAGGAATTCATGATCTGCCTGTTTGTCTTGCTGGATTTTGGTGGTGTTCCGCCACCGGGAGAGTAATCTCCGGTCATCTTCAAGGGCAAGGAGGTGAGGATCGAGCTTGGGTCGTTTGGGCCTCAGTTCGAATATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

174

Amino Acids

19.07

Weight (kDa)

7.64

Isoelectric Point (pI)

49.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 80
AccBSI CCGCTC 1 cut(s) 97
AciI CCGC 4 cut(s) 97, 100, 196, 432
AclWI GGATC 2 cut(s) 64, 489
AcoI YGGCCR 1 cut(s) 166
AcsI RAATTY 1 cut(s) 387
AcyI GRCGYC 1 cut(s) 141
AdeI CACNNNGTG 1 cut(s) 80
AfiI CCNNNNNNNGG 4 cut(s) 80, 161, 232, 438
AgsI TTSAA 3 cut(s) 61, 149, 464
AloI GAACNNNNNNTCC 2 cut(s) 303, 335
AluBI AGCT 1 cut(s) 488
AluI AGCT 1 cut(s) 488
AlwI GGATC 2 cut(s) 64, 489
AoxI GGCC 2 cut(s) 166, 501
ApeKI GCWGC 2 cut(s) 8, 11
ApoI RAATTY 1 cut(s) 387
AseI ATTAAT 1 cut(s) 129
AspLEI GCGC 1 cut(s) 86
AspS9I GGNCC 1 cut(s) 501
AsuC2I CCSGG 1 cut(s) 439
AsuHPI GGTGA 2 cut(s) 130, 488
AsuII TTCGAA 1 cut(s) 511
BaeGI GKGCMC 1 cut(s) 156
BalI TGGCCA 1 cut(s) 168
BccI CCATC 3 cut(s) 168, 279, 338
BcnI CCSGG 1 cut(s) 439
BfaI CTAG 1 cut(s) 35
BfoI RGCGCY 1 cut(s) 87
BglII AGATCT 2 cut(s) 359, 370
BisI GCNGC 4 cut(s) 9, 12, 98, 101
BlsI GCNGC 4 cut(s) 10, 13, 99, 102
Bme1390I CCNGG 1 cut(s) 439
BmgT120I GGNCC 1 cut(s) 501
BmiI GGNNCC 2 cut(s) 218, 224
BmrFI CCNGG 1 cut(s) 439
BmsI GCATC 1 cut(s) 302
Bpu14I TTCGAA 1 cut(s) 511
BpuMI CCSGG 1 cut(s) 439
BsaHI GRCGYC 1 cut(s) 141
BsaWI WCCGGW 1 cut(s) 452
Bsc4I CCNNNNNNNGG 4 cut(s) 80, 161, 232, 438
Bse3DI GCAATG 2 cut(s) 166, 204
BseGI GGATG 1 cut(s) 317
BseLI CCNNNNNNNGG 4 cut(s) 80, 161, 232, 438
BseMI GCAATG 2 cut(s) 166, 204
BseMII CTCAG 1 cut(s) 519
BseSI GKGCMC 1 cut(s) 156
BseYI CCCAGC 2 cut(s) 103, 155
BsgI GTGCAG 1 cut(s) 111
BshFI GGCC 2 cut(s) 168, 503
BsiSI CCGG 3 cut(s) 220, 438, 453
BslI CCNNNNNNNGG 4 cut(s) 80, 161, 232, 438
BsnI GGCC 2 cut(s) 168, 503
Bsp119I TTCGAA 1 cut(s) 511
Bsp1286I GDGCHC 1 cut(s) 156
Bsp143I GATC 9 cut(s) 69, 112, 247, 271, 334, 359, 370, 394, 481
BspACI CCGC 4 cut(s) 97, 100, 196, 432
BspANI GGCC 2 cut(s) 168, 503
BspCNI CTCAG 1 cut(s) 518
BspHI TCATGA 2 cut(s) 244, 391
BspLI GGNNCC 2 cut(s) 218, 224
BspPI GGATC 2 cut(s) 64, 489
BspT104I TTCGAA 1 cut(s) 511
BsrBI CCGCTC 1 cut(s) 97
BsrDI GCAATG 2 cut(s) 166, 204
BssMI GATC 9 cut(s) 69, 112, 247, 271, 334, 359, 370, 394, 481
BssNI GRCGYC 1 cut(s) 141
Bst6I CTCTTC 1 cut(s) 23
BstACI GRCGYC 1 cut(s) 141
BstBI TTCGAA 1 cut(s) 511
BstDEI CTNAG 1 cut(s) 505
BstF5I GGATG 1 cut(s) 317
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 1 cut(s) 86
BstKTI GATC 9 cut(s) 72, 115, 250, 274, 337, 362, 373, 397, 484
BstMBI GATC 9 cut(s) 69, 112, 247, 271, 334, 359, 370, 394, 481
BstMWI GCNNNNNNNGC 1 cut(s) 165
BstSCI CCNGG 1 cut(s) 437
BstSLI GKGCMC 1 cut(s) 156
BstX2I RGATCY 2 cut(s) 359, 370
BstYI RGATCY 2 cut(s) 359, 370
BsuRI GGCC 2 cut(s) 168, 503
BtsCI GGATG 1 cut(s) 317
BtsIMutI CAGTG 1 cut(s) 344
CciI TCATGA 2 cut(s) 244, 391
CfoI GCGC 1 cut(s) 86
Cfr13I GGNCC 1 cut(s) 501
CseI GACGC 1 cut(s) 130
CspCI CAANNNNNGTGG 2 cut(s) 158, 193
CviAII CATG 4 cut(s) 52, 67, 245, 392
CviJI RGCY 7 cut(s) 65, 103, 168, 219, 225, 488, 503
CviKI_1 RGCY 7 cut(s) 65, 103, 168, 219, 225, 488, 503
DdeI CTNAG 1 cut(s) 505
DpnI GATC 9 cut(s) 71, 114, 249, 273, 336, 361, 372, 396, 483
DpnII GATC 9 cut(s) 69, 112, 247, 271, 334, 359, 370, 394, 481
DraIII CACNNNGTG 1 cut(s) 80
EaeI YGGCCR 1 cut(s) 166
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
EciI GGCGGA 1 cut(s) 421
EcoRI GAATTC 1 cut(s) 387
FaeI CATG 4 cut(s) 55, 70, 248, 395
FaiI YATR 6 cut(s) 53, 68, 246, 365, 393, 517
FatI CATG 4 cut(s) 51, 66, 244, 391
Fnu4HI GCNGC 4 cut(s) 9, 12, 98, 101
FokI GGATG 1 cut(s) 324
Fsp4HI GCNGC 4 cut(s) 9, 12, 98, 101
FspBI CTAG 1 cut(s) 35
GlaI GCGC 1 cut(s) 85
GluI GCNGC 4 cut(s) 9, 12, 98, 101
GsaI CCCAGC 2 cut(s) 107, 159
HaeII RGCGCY 1 cut(s) 87
HaeIII GGCC 2 cut(s) 168, 503
HapII CCGG 3 cut(s) 220, 438, 453
HgaI GACGC 1 cut(s) 130
HhaI GCGC 1 cut(s) 86
Hin1I GRCGYC 1 cut(s) 141
Hin1II CATG 4 cut(s) 55, 70, 248, 395
Hin6I GCGC 1 cut(s) 84
HinP1I GCGC 1 cut(s) 84
HinfI GANTC 3 cut(s) 186, 201, 305
HpaII CCGG 3 cut(s) 220, 438, 453
HphI GGTGA 2 cut(s) 130, 488
Hpy188I TCNGA 1 cut(s) 185
Hpy188III TCNNGA 6 cut(s) 110, 245, 297, 309, 332, 392
Hpy99I CGWCG 2 cut(s) 146, 285
HpyAV CCTTC 1 cut(s) 233
HpyCH4V TGCA 3 cut(s) 14, 92, 209
HpyF10VI GCNNNNNNNGC 1 cut(s) 165
HpyF3I CTNAG 1 cut(s) 505
Hsp92I GRCGYC 1 cut(s) 141
Hsp92II CATG 4 cut(s) 55, 70, 248, 395
HspAI GCGC 1 cut(s) 84
Kzo9I GATC 9 cut(s) 69, 112, 247, 271, 334, 359, 370, 394, 481
LmnI GCTCC 2 cut(s) 216, 222
LweI GCATC 1 cut(s) 302
MaeI CTAG 1 cut(s) 35
MalI GATC 9 cut(s) 71, 114, 249, 273, 336, 361, 372, 396, 483
MbiI CCGCTC 1 cut(s) 97
MboI GATC 9 cut(s) 69, 112, 247, 271, 334, 359, 370, 394, 481
MboII GAAGA 3 cut(s) 10, 380, 452
MflI RGATCY 2 cut(s) 359, 370
MhlI GDGCHC 1 cut(s) 156
MlsI TGGCCA 1 cut(s) 168
MluCI AATT 2 cut(s) 300, 387
MluNI TGGCCA 1 cut(s) 168
MlyI GAGTC 3 cut(s) 195, 210, 314
MnlI CCTC 4 cut(s) 377, 467, 472, 514
Mox20I TGGCCA 1 cut(s) 168
MscI TGGCCA 1 cut(s) 168
MseI TTAA 1 cut(s) 129
Msp20I TGGCCA 1 cut(s) 168
MspI CCGG 3 cut(s) 220, 438, 453
MspR9I CCNGG 1 cut(s) 439
MwoI GCNNNNNNNGC 1 cut(s) 165
NciI CCSGG 1 cut(s) 439
NdeII GATC 9 cut(s) 69, 112, 247, 271, 334, 359, 370, 394, 481
NlaIII CATG 4 cut(s) 55, 70, 248, 395
NlaIV GGNNCC 2 cut(s) 218, 224
NspV TTCGAA 1 cut(s) 511
PagI TCATGA 2 cut(s) 244, 391
PcsI WCGNNNNNNNCGW 1 cut(s) 280
PflMI CCANNNNNTGG 1 cut(s) 80
PkrI GCNGC 4 cut(s) 10, 13, 99, 102
PleI GAGTC 3 cut(s) 194, 209, 313
PpsI GAGTC 3 cut(s) 194, 209, 313
PshBI ATTAAT 1 cut(s) 129
PspFI CCCAGC 2 cut(s) 103, 155
PspN4I GGNNCC 2 cut(s) 218, 224
PspPI GGNCC 1 cut(s) 501
PsuI RGATCY 2 cut(s) 359, 370
SaqAI TTAA 1 cut(s) 129
SatI GCNGC 4 cut(s) 9, 12, 98, 101
Sau3AI GATC 9 cut(s) 69, 112, 247, 271, 334, 359, 370, 394, 481
Sau96I GGNCC 1 cut(s) 501
SchI GAGTC 3 cut(s) 195, 210, 314
ScrFI CCNGG 1 cut(s) 439
SduI GDGCHC 1 cut(s) 156
SetI ASST 3 cut(s) 244, 478, 490
SfaNI GCATC 1 cut(s) 302
SfuI TTCGAA 1 cut(s) 511
Sse9I AATT 2 cut(s) 300, 387
SsiI CCGC 4 cut(s) 97, 100, 196, 432
SspMI CTAG 1 cut(s) 35
StyD4I CCNGG 1 cut(s) 437
TaqI TCGA 2 cut(s) 484, 511
TasI AATT 2 cut(s) 300, 387
TauI GCSGC 2 cut(s) 100, 103
Tru1I TTAA 1 cut(s) 129
Tru9I TTAA 1 cut(s) 129
TscAI CASTG 1 cut(s) 344
TseI GCWGC 2 cut(s) 8, 11
TspDTI ATGAA 1 cut(s) 380
TspRI CASTG 1 cut(s) 344
Van91I CCANNNNNTGG 1 cut(s) 80
VspI ATTAAT 1 cut(s) 129
XapI RAATTY 1 cut(s) 387
XspI CTAG 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.