RLG00000032526

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0014842
Physical Location & Seq
Forward (+)
16110153 .. 16111636
1484 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000032526

Sequence Viewer

Length: 414 bp
ATGATCCATGGGAAGAAGATCCGGATTTCGGCTCGGGCAGCGACTTCCTTCCTACAAGGATTGGCGGATTTTCAATGGCGGAACTGGACTGTTGGTAGTGCTGGTGCAGAGCGGCGGCTGAGTCTTGATCTGGTGTTTGGGATTGATGCTAGCGGCATCGTTGAAGTGCCCAGCGATGGTGGCCAACGATGGTGGTTCGGAGTCAATAACCGCAGAGTCTTTGCAGAATGGAACCGTAGTCTTGCTTTGGTAGAAGGTCATGATCCTGTGGTTGTTGTGGGTGCAGATTGGCGGCGGCGACATGATTGGTCAAGCATTGAGTCTGGGTGCCTTGTTCTTGGGTGTCTAGATCAGTGTGATGTGGTACTTATGAGATTTATGGAAGATCTGGGTTTGGGACCCAGGAATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

138

Amino Acids

15.33

Weight (kDa)

6.82

Isoelectric Point (pI)

45.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 327
AccBSI CCGCTC 1 cut(s) 112
AccIII TCCGGA 1 cut(s) 21
AciI CCGC 8 cut(s) 65, 79, 112, 115, 153, 211, 292, 295
AclWI GGATC 2 cut(s) 13, 257
AcoI YGGCCR 1 cut(s) 181
AcsI RAATTY 1 cut(s) 406
AfaI GTAC 1 cut(s) 366
AfiI CCNNNNNNNGG 2 cut(s) 28, 176
AgsI TTSAA 2 cut(s) 74, 164
AjnI CCWGG 1 cut(s) 401
AlwI GGATC 2 cut(s) 13, 257
Ama87I CYCGRG 1 cut(s) 33
Aor13HI TCCGGA 1 cut(s) 21
AoxI GGCC 1 cut(s) 181
ApeKI GCWGC 1 cut(s) 38
ApoI RAATTY 1 cut(s) 406
AspS9I GGNCC 1 cut(s) 398
AsuNHI GCTAGC 1 cut(s) 149
AvaI CYCGRG 1 cut(s) 33
AvaII GGWCC 1 cut(s) 398
BaeGI GKGCMC 1 cut(s) 171
BalI TGGCCA 1 cut(s) 183
BanI GGYRCC 1 cut(s) 327
BbvI GCAGC 1 cut(s) 50
BccI CCATC 2 cut(s) 170, 183
BciT130I CCWGG 1 cut(s) 403
BfaI CTAG 2 cut(s) 150, 347
BglII AGATCT 1 cut(s) 385
BisI GCNGC 6 cut(s) 39, 113, 116, 154, 293, 296
BlsI GCNGC 6 cut(s) 40, 114, 117, 155, 294, 297
Bme1390I CCNGG 1 cut(s) 403
Bme18I GGWCC 1 cut(s) 398
BmeT110I CYCGRG 1 cut(s) 33
BmgT120I GGNCC 1 cut(s) 398
BmiI GGNNCC 4 cut(s) 233, 329, 399, 400
BmrFI CCNGG 1 cut(s) 403
BmsI GCATC 2 cut(s) 136, 165
BmtI GCTAGC 1 cut(s) 153
BsaJI CCNNGG 2 cut(s) 7, 401
BsaWI WCCGGW 1 cut(s) 21
Bsc4I CCNNNNNNNGG 2 cut(s) 28, 176
Bse1I ACTGG 1 cut(s) 89
BseAI TCCGGA 1 cut(s) 21
BseBI CCWGG 1 cut(s) 403
BseDI CCNNGG 2 cut(s) 7, 401
BseLI CCNNNNNNNGG 2 cut(s) 28, 176
BseMII CTCAG 1 cut(s) 110
BseNI ACTGG 1 cut(s) 89
BseSI GKGCMC 1 cut(s) 171
BseXI GCAGC 1 cut(s) 50
BseYI CCCAGC 1 cut(s) 170
BsgI GTGCAG 2 cut(s) 126, 303
BshFI GGCC 1 cut(s) 183
BshNI GGYRCC 1 cut(s) 327
BsiHKCI CYCGRG 1 cut(s) 33
BsiSI CCGG 1 cut(s) 22
BslI CCNNNNNNNGG 2 cut(s) 28, 176
BsnI GGCC 1 cut(s) 183
BsoBI CYCGRG 1 cut(s) 33
Bsp1286I GDGCHC 1 cut(s) 171
Bsp13I TCCGGA 1 cut(s) 21
Bsp143I GATC 6 cut(s) 3, 18, 127, 262, 349, 385
Bsp19I CCATGG 1 cut(s) 7
BspACI CCGC 8 cut(s) 65, 79, 112, 115, 153, 211, 292, 295
BspANI GGCC 1 cut(s) 183
BspCNI CTCAG 1 cut(s) 111
BspEI TCCGGA 1 cut(s) 21
BspHI TCATGA 2 cut(s) 259, 410
BspLI GGNNCC 4 cut(s) 233, 329, 399, 400
BspOI GCTAGC 1 cut(s) 153
BspPI GGATC 2 cut(s) 13, 257
BspT107I GGYRCC 1 cut(s) 327
BsrBI CCGCTC 1 cut(s) 112
BsrI ACTGG 1 cut(s) 89
BssECI CCNNGG 2 cut(s) 7, 401
BssMI GATC 6 cut(s) 3, 18, 127, 262, 349, 385
BssT1I CCWWGG 1 cut(s) 7
Bst2UI CCWGG 1 cut(s) 403
Bst4CI ACNGT 2 cut(s) 91, 236
BstC8I GCNNGC 1 cut(s) 151
BstDEI CTNAG 1 cut(s) 119
BstDSI CCRYGG 1 cut(s) 7
BstKTI GATC 6 cut(s) 6, 21, 130, 265, 352, 388
BstMBI GATC 6 cut(s) 3, 18, 127, 262, 349, 385
BstMWI GCNNNNNNNGC 2 cut(s) 38, 180
BstNI CCWGG 1 cut(s) 403
BstSCI CCNGG 1 cut(s) 401
BstSLI GKGCMC 1 cut(s) 171
BstV1I GCAGC 1 cut(s) 50
BstX2I RGATCY 2 cut(s) 18, 385
BstYI RGATCY 2 cut(s) 18, 385
BsuRI GGCC 1 cut(s) 183
BtgI CCRYGG 1 cut(s) 7
BtgZI GCGATG 1 cut(s) 189
BtsIMutI CAGTG 1 cut(s) 359
Cac8I GCNNGC 1 cut(s) 151
CciI TCATGA 2 cut(s) 259, 410
Cfr13I GGNCC 1 cut(s) 398
Csp6I GTAC 1 cut(s) 365
CspCI CAANNNNNGTGG 2 cut(s) 173, 208
CviAII CATG 4 cut(s) 8, 260, 302, 411
CviJI RGCY 3 cut(s) 32, 118, 183
CviKI_1 RGCY 3 cut(s) 32, 118, 183
CviQI GTAC 1 cut(s) 365
DdeI CTNAG 1 cut(s) 119
DpnI GATC 6 cut(s) 5, 20, 129, 264, 351, 387
DpnII GATC 6 cut(s) 3, 18, 127, 262, 349, 385
EaeI YGGCCR 1 cut(s) 181
EciI GGCGGA 2 cut(s) 80, 94
Eco130I CCWWGG 1 cut(s) 7
Eco47I GGWCC 1 cut(s) 398
Eco88I CYCGRG 1 cut(s) 33
EcoO109I RGGNCCY 1 cut(s) 398
EcoRI GAATTC 1 cut(s) 406
EcoRII CCWGG 1 cut(s) 401
EcoT14I CCWWGG 1 cut(s) 7
ErhI CCWWGG 1 cut(s) 7
FaeI CATG 4 cut(s) 11, 263, 305, 414
FaiI YATR 6 cut(s) 9, 261, 303, 371, 380, 412
FatI CATG 4 cut(s) 7, 259, 301, 410
Fnu4HI GCNGC 6 cut(s) 39, 113, 116, 154, 293, 296
Fsp4HI GCNGC 6 cut(s) 39, 113, 116, 154, 293, 296
FspBI CTAG 2 cut(s) 150, 347
GluI GCNGC 6 cut(s) 39, 113, 116, 154, 293, 296
GsaI CCCAGC 1 cut(s) 174
HaeIII GGCC 1 cut(s) 183
HapII CCGG 1 cut(s) 22
Hin1II CATG 4 cut(s) 11, 263, 305, 414
HinfI GANTC 4 cut(s) 121, 201, 216, 320
HpaII CCGG 1 cut(s) 22
Hpy188I TCNGA 1 cut(s) 200
Hpy188III TCNNGA 5 cut(s) 22, 125, 260, 347, 411
HpyAV CCTTC 2 cut(s) 58, 248
HpyCH4III ACNGT 2 cut(s) 91, 236
HpyCH4V TGCA 3 cut(s) 107, 224, 284
HpyF10VI GCNNNNNNNGC 2 cut(s) 38, 180
HpyF3I CTNAG 1 cut(s) 119
Hsp92II CATG 4 cut(s) 11, 263, 305, 414
KflI GGGWCCC 1 cut(s) 398
Kpn2I TCCGGA 1 cut(s) 21
Kzo9I GATC 6 cut(s) 3, 18, 127, 262, 349, 385
LpnPI CCDG 9 cut(s) 35, 70, 87, 116, 184, 279, 309, 374, 388
Lsp1109I GCAGC 1 cut(s) 50
LweI GCATC 2 cut(s) 136, 165
MaeI CTAG 2 cut(s) 150, 347
MalI GATC 6 cut(s) 5, 20, 129, 264, 351, 387
MbiI CCGCTC 1 cut(s) 112
MboI GATC 6 cut(s) 3, 18, 127, 262, 349, 385
MboII GAAGA 3 cut(s) 25, 28, 395
MflI RGATCY 2 cut(s) 18, 385
MhlI GDGCHC 1 cut(s) 171
MlsI TGGCCA 1 cut(s) 183
MluCI AATT 1 cut(s) 406
MluNI TGGCCA 1 cut(s) 183
MlyI GAGTC 4 cut(s) 130, 210, 225, 329
Mox20I TGGCCA 1 cut(s) 183
MroI TCCGGA 1 cut(s) 21
MscI TGGCCA 1 cut(s) 183
Msp20I TGGCCA 1 cut(s) 183
MspI CCGG 1 cut(s) 22
MspR9I CCNGG 1 cut(s) 403
MvaI CCWGG 1 cut(s) 403
MwoI GCNNNNNNNGC 2 cut(s) 38, 180
NcoI CCATGG 1 cut(s) 7
NdeII GATC 6 cut(s) 3, 18, 127, 262, 349, 385
NheI GCTAGC 1 cut(s) 149
NlaIII CATG 4 cut(s) 11, 263, 305, 414
NlaIV GGNNCC 4 cut(s) 233, 329, 399, 400
PagI TCATGA 2 cut(s) 259, 410
PkrI GCNGC 6 cut(s) 40, 114, 117, 155, 294, 297
PleI GAGTC 4 cut(s) 129, 209, 224, 328
PpsI GAGTC 4 cut(s) 129, 209, 224, 328
PpuMI RGGWCCY 1 cut(s) 398
Psp5II RGGWCCY 1 cut(s) 398
Psp6I CCWGG 1 cut(s) 401
PspFI CCCAGC 1 cut(s) 170
PspGI CCWGG 1 cut(s) 401
PspN4I GGNNCC 4 cut(s) 233, 329, 399, 400
PspPI GGNCC 1 cut(s) 398
PspPPI RGGWCCY 1 cut(s) 398
PsuI RGATCY 2 cut(s) 18, 385
RsaI GTAC 1 cut(s) 366
RsaNI GTAC 1 cut(s) 365
SatI GCNGC 6 cut(s) 39, 113, 116, 154, 293, 296
Sau3AI GATC 6 cut(s) 3, 18, 127, 262, 349, 385
Sau96I GGNCC 1 cut(s) 398
SchI GAGTC 4 cut(s) 130, 210, 225, 329
ScrFI CCNGG 1 cut(s) 403
SduI GDGCHC 1 cut(s) 171
SetI ASST 1 cut(s) 259
SfaNI GCATC 2 cut(s) 136, 165
SinI GGWCC 1 cut(s) 398
Sse9I AATT 1 cut(s) 406
SsiI CCGC 8 cut(s) 65, 79, 112, 115, 153, 211, 292, 295
SspMI CTAG 2 cut(s) 150, 347
StyD4I CCNGG 1 cut(s) 401
StyI CCWWGG 1 cut(s) 7
TaaI ACNGT 2 cut(s) 91, 236
TasI AATT 1 cut(s) 406
TauI GCSGC 5 cut(s) 115, 118, 156, 295, 298
TscAI CASTG 1 cut(s) 359
TseI GCWGC 1 cut(s) 38
TspDTI ATGAA 1 cut(s) 399
TspRI CASTG 1 cut(s) 359
VpaK11BI GGWCC 1 cut(s) 398
XapI RAATTY 1 cut(s) 406
XbaI TCTAGA 1 cut(s) 346
XspI CTAG 2 cut(s) 150, 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.