RLG00000001224

peroxisome matrix targeting signal-2 binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0009734
Physical Location & Seq
Forward (+)
12308618 .. 12310494
1877 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001224

Sequence Viewer

Length: 486 bp
ATGATTATCCCAGCGCATGAGTTCGAGATCCTCTCCTGCGATTGGAACAAGAACGACGAATGCGTCATCGCCACCACCTCCGTCGACAAATCGATCAAGGTTTGGCACGCCAGGTACATCGGAGCTCCGATTTGGTGGTCAACGGCCACAGCTACGCCGTCAGAAAAGCCAAATTCTCGCCGCACCGGCAAACTACAGCTCCGGCCTTGGATCAATGACTCTGAAAACTACGTTGCCGTCGCCGTCGATTATCGCCTGGTGATTCGCGACACTCTCTCCTTCAAGGTACCTGATTTGGAGCTGTATGAGAACAGAGACGAGGCTGCCAAAAGAGAAGAGGTTCTTAGCTGCATTGGTCAGGTGATGTTGGCGTGTGGGAGTTTCTTCTTATTGCTGCAATGTTCTCTCAACATGCTCAAACGAGATACTGGGAATCAATTACATAAGCATTTAGGTGACACTGGACATGCTTCACTGAAGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000151 GO:0000268 GO:0001501 GO:0001503 GO:0001764 GO:0001958 GO:0003674 GO:0005048 GO:0005053 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005777 GO:0005778 GO:0005782 GO:0005829 GO:0006082 GO:0006605 GO:0006625 GO:0006629 GO:0006631 GO:0006635 GO:0006662 GO:0006810 GO:0006886 GO:0006928 GO:0006996 GO:0007031 GO:0007275 GO:0007399 GO:0008104 GO:0008150 GO:0008152 GO:0008610 GO:0008611 GO:0009056 GO:0009058 GO:0009062 GO:0009653 GO:0009887 GO:0009987 GO:0015031 GO:0015833 GO:0015919 GO:0016020 GO:0016042 GO:0016043 GO:0016054 GO:0016477 GO:0016558 GO:0016560 GO:0017038 GO:0018904 GO:0019395 GO:0019752 GO:0019899 GO:0022008 GO:0030154 GO:0030258 GO:0031090 GO:0031461 GO:0031903 GO:0031907 GO:0031974 GO:0032501 GO:0032502 GO:0032787 GO:0032991 GO:0033036 GO:0033218 GO:0033365 GO:0034440 GO:0034613 GO:0036075 GO:0040011 GO:0042277 GO:0042579 GO:0042802 GO:0042803 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043574 GO:0044237 GO:0044238 GO:0044242 GO:0044248 GO:0044249 GO:0044255 GO:0044281 GO:0044282 GO:0044422 GO:0044424 GO:0044438 GO:0044439 GO:0044444 GO:0044446 GO:0044464 GO:0044743 GO:0045184 GO:0046395 GO:0046485 GO:0046504 GO:0046907 GO:0046983 GO:0048513 GO:0048699 GO:0048705 GO:0048731 GO:0048856 GO:0048869 GO:0048870 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0051674 GO:0055085 GO:0055114 GO:0060348 GO:0060349 GO:0060350 GO:0065002 GO:0070013 GO:0070727 GO:0071702 GO:0071704 GO:0071705 GO:0071806 GO:0071840 GO:0072329 GO:0072594 GO:0072662 GO:0072663 GO:0080008 GO:0097384 GO:0098588 GO:0098805 GO:1901503 GO:1901575 GO:1901576 GO:1902494 GO:1990234
KEGG Pathways
Metabolic & Signaling
ko04146 map04146
Pfam Domains
Protein Families
WD40

Protein Analysis

162

Amino Acids

18.33

Weight (kDa)

6.95

Isoelectric Point (pI)

37.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 62
Acc65I GGTACC 1 cut(s) 286
AccB1I GGYRCC 1 cut(s) 286
AccI GTMKAC 1 cut(s) 84
AccII CGCG 1 cut(s) 267
AciI CCGC 1 cut(s) 181
AclWI GGATC 2 cut(s) 22, 218
AcoI YGGCCR 1 cut(s) 144
AcsI RAATTY 1 cut(s) 172
AfaI GTAC 2 cut(s) 116, 288
AfiI CCNNNNNNNGG 1 cut(s) 42
AgsI TTSAA 1 cut(s) 283
AjnI CCWGG 2 cut(s) 110, 255
AluBI AGCT 5 cut(s) 125, 152, 199, 301, 348
AluI AGCT 5 cut(s) 125, 152, 199, 301, 348
Alw21I GWGCWC 1 cut(s) 127
Alw26I GTCTC 1 cut(s) 309
AlwI GGATC 2 cut(s) 22, 218
AoxI GGCC 2 cut(s) 144, 203
ApeKI GCWGC 3 cut(s) 323, 348, 394
ApoI RAATTY 1 cut(s) 172
Asp700I GAANNNNTTC 1 cut(s) 339
Asp718I GGTACC 1 cut(s) 286
AspLEI GCGC 1 cut(s) 16
AsuHPI GGTGA 3 cut(s) 271, 373, 467
BanI GGYRCC 1 cut(s) 286
BanII GRGCYC 1 cut(s) 127
Bbv12I GWGCWC 1 cut(s) 127
BbvI GCAGC 3 cut(s) 310, 335, 381
BceAI ACGGC 4 cut(s) 142, 159, 221, 227
BciT130I CCWGG 2 cut(s) 112, 257
BcoDI GTCTC 1 cut(s) 309
BfmI CTRYAG 1 cut(s) 194
BglI GCCNNNNNGGC 1 cut(s) 186
BisI GCNGC 4 cut(s) 181, 324, 349, 395
BlsI GCNGC 4 cut(s) 182, 325, 350, 396
Bme1390I CCNGG 2 cut(s) 112, 257
BmiI GGNNCC 1 cut(s) 288
BmrFI CCNGG 2 cut(s) 112, 257
BmrI ACTGGG 1 cut(s) 438
BmuI ACTGGG 1 cut(s) 438
BplI GAGNNNNNCTC 2 cut(s) 17, 49
Bsa29I ATCGAT 1 cut(s) 92
BsaJI CCNNGG 1 cut(s) 206
BsaXI ACNNNNNCTCC 4 cut(s) 183, 213, 260, 290
Bsc4I CCNNNNNNNGG 1 cut(s) 42
Bse118I RCCGGY 1 cut(s) 185
Bse1I ACTGG 2 cut(s) 433, 466
Bse3DI GCAATG 1 cut(s) 404
BseBI CCWGG 2 cut(s) 112, 257
BseCI ATCGAT 1 cut(s) 92
BseDI CCNNGG 1 cut(s) 206
BseLI CCNNNNNNNGG 1 cut(s) 42
BseMI GCAATG 1 cut(s) 404
BseNI ACTGG 2 cut(s) 433, 466
BseXI GCAGC 3 cut(s) 310, 335, 381
BseYI CCCAGC 1 cut(s) 10
Bsh1236I CGCG 1 cut(s) 267
BshFI GGCC 2 cut(s) 146, 205
BshNI GGYRCC 1 cut(s) 286
BshVI ATCGAT 1 cut(s) 92
BsiHKAI GWGCWC 1 cut(s) 127
BsiSI CCGG 2 cut(s) 186, 202
BslI CCNNNNNNNGG 1 cut(s) 42
BsmAI GTCTC 1 cut(s) 309
BsmBI CGTCTC 1 cut(s) 309
BsmI GAATGC 1 cut(s) 65
BsnI GGCC 2 cut(s) 146, 205
Bsp1286I GDGCHC 1 cut(s) 127
Bsp143I GATC 3 cut(s) 27, 93, 210
Bsp68I TCGCGA 1 cut(s) 267
BspACI CCGC 1 cut(s) 181
BspANI GGCC 2 cut(s) 146, 205
BspDI ATCGAT 1 cut(s) 92
BspFNI CGCG 1 cut(s) 267
BspLI GGNNCC 1 cut(s) 288
BspPI GGATC 2 cut(s) 22, 218
BspT107I GGYRCC 1 cut(s) 286
BsrDI GCAATG 1 cut(s) 404
BsrFI RCCGGY 1 cut(s) 185
BsrI ACTGG 2 cut(s) 433, 466
BssAI RCCGGY 1 cut(s) 185
BssECI CCNNGG 1 cut(s) 206
BssMI GATC 3 cut(s) 27, 93, 210
BssT1I CCWWGG 1 cut(s) 206
Bst2UI CCWGG 2 cut(s) 112, 257
Bst6I CTCTTC 2 cut(s) 330, 473
BstC8I GCNNGC 1 cut(s) 108
BstDEI CTNAG 1 cut(s) 344
BstFNI CGCG 1 cut(s) 267
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 3 cut(s) 30, 96, 213
BstMAI GTCTC 1 cut(s) 309
BstMBI GATC 3 cut(s) 27, 93, 210
BstMWI GCNNNNNNNGC 1 cut(s) 186
BstNI CCWGG 2 cut(s) 112, 257
BstNSI RCATGY 2 cut(s) 415, 470
BstSCI CCNGG 2 cut(s) 110, 255
BstSFI CTRYAG 1 cut(s) 194
BstUI CGCG 1 cut(s) 267
BstV1I GCAGC 3 cut(s) 310, 335, 381
BstX2I RGATCY 1 cut(s) 27
BstYI RGATCY 1 cut(s) 27
Bsu15I ATCGAT 1 cut(s) 92
BsuRI GGCC 2 cut(s) 146, 205
BsuTUI ATCGAT 1 cut(s) 92
BtgZI GCGATG 1 cut(s) 52
BtsIMutI CAGTG 2 cut(s) 459, 473
BtuMI TCGCGA 1 cut(s) 267
Cac8I GCNNGC 1 cut(s) 108
CfoI GCGC 1 cut(s) 16
Cfr10I RCCGGY 1 cut(s) 185
ClaI ATCGAT 1 cut(s) 92
CseI GACGC 1 cut(s) 52
Csp6I GTAC 2 cut(s) 115, 287
CviAII CATG 3 cut(s) 17, 412, 467
CviJI RGCY 9 cut(s) 125, 146, 152, 169, 199, 205, 301, 323, 348
CviKI_1 RGCY 9 cut(s) 125, 146, 152, 169, 199, 205, 301, 323, 348
CviQI GTAC 2 cut(s) 115, 287
DdeI CTNAG 1 cut(s) 344
DpnI GATC 3 cut(s) 29, 95, 212
DpnII GATC 3 cut(s) 27, 93, 210
DrdI GACNNNNNNGTC 1 cut(s) 62
DseDI GACNNNNNNGTC 1 cut(s) 62
EaeI YGGCCR 1 cut(s) 144
Eam1104I CTCTTC 2 cut(s) 330, 473
EarI CTCTTC 2 cut(s) 330, 473
Ecl136II GAGCTC 1 cut(s) 125
Eco130I CCWWGG 1 cut(s) 206
Eco24I GRGCYC 1 cut(s) 127
Eco53kI GAGCTC 1 cut(s) 125
EcoICRI GAGCTC 1 cut(s) 125
EcoRII CCWGG 2 cut(s) 110, 255
EcoT14I CCWWGG 1 cut(s) 206
EcoT38I GRGCYC 1 cut(s) 127
ErhI CCWWGG 1 cut(s) 206
Esp3I CGTCTC 1 cut(s) 309
FaeI CATG 3 cut(s) 20, 415, 470
FaiI YATR 5 cut(s) 18, 306, 413, 444, 468
FalI AAGNNNNNCTT 2 cut(s) 327, 359
FatI CATG 3 cut(s) 16, 411, 466
FblI GTMKAC 1 cut(s) 84
Fnu4HI GCNGC 4 cut(s) 181, 324, 349, 395
FriOI GRGCYC 1 cut(s) 127
Fsp4HI GCNGC 4 cut(s) 181, 324, 349, 395
GlaI GCGC 1 cut(s) 15
GluI GCNGC 4 cut(s) 181, 324, 349, 395
GsaI CCCAGC 1 cut(s) 14
HaeIII GGCC 2 cut(s) 146, 205
HapII CCGG 2 cut(s) 186, 202
HgaI GACGC 1 cut(s) 52
HhaI GCGC 1 cut(s) 16
Hin1II CATG 3 cut(s) 20, 415, 470
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HincII GTYRAC 2 cut(s) 85, 141
HindII GTYRAC 2 cut(s) 85, 141
HinfI GANTC 3 cut(s) 218, 262, 433
HpaII CCGG 2 cut(s) 186, 202
HphI GGTGA 3 cut(s) 271, 373, 467
Hpy166II GTNNAC 2 cut(s) 85, 141
Hpy188I TCNGA 4 cut(s) 122, 129, 163, 223
Hpy188III TCNNGA 2 cut(s) 25, 266
Hpy8I GTNNAC 2 cut(s) 85, 141
Hpy99I CGWCG 4 cut(s) 59, 86, 242, 248
HpyAV CCTTC 1 cut(s) 289
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 2 cut(s) 351, 397
HpyF10VI GCNNNNNNNGC 1 cut(s) 186
HpyF3I CTNAG 1 cut(s) 344
HpySE526I ACGT 1 cut(s) 231
Hsp92II CATG 3 cut(s) 20, 415, 470
HspAI GCGC 1 cut(s) 14
KpnI GGTACC 1 cut(s) 290
Kzo9I GATC 3 cut(s) 27, 93, 210
LmnI GCTCC 4 cut(s) 122, 130, 204, 298
Lsp1109I GCAGC 3 cut(s) 310, 335, 381
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 1 cut(s) 455
MalI GATC 3 cut(s) 29, 95, 212
MboI GATC 3 cut(s) 27, 93, 210
MboII GAAGA 2 cut(s) 347, 376
MflI RGATCY 1 cut(s) 27
MhlI GDGCHC 1 cut(s) 127
MluCI AATT 2 cut(s) 172, 437
MlyI GAGTC 1 cut(s) 212
MnlI CCTC 5 cut(s) 41, 88, 313, 331, 474
MroXI GAANNNNTTC 1 cut(s) 339
MslI CAYNNNNRTG 1 cut(s) 453
MspI CCGG 2 cut(s) 186, 202
MspR9I CCNGG 2 cut(s) 112, 257
Mva1269I GAATGC 1 cut(s) 65
MvaI CCWGG 2 cut(s) 112, 257
MvnI CGCG 1 cut(s) 267
MwoI GCNNNNNNNGC 1 cut(s) 186
NdeII GATC 3 cut(s) 27, 93, 210
NlaIII CATG 3 cut(s) 20, 415, 470
NlaIV GGNNCC 1 cut(s) 288
NmuCI GTSAC 1 cut(s) 455
NruI TCGCGA 1 cut(s) 267
NspI RCATGY 2 cut(s) 415, 470
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PctI GAATGC 1 cut(s) 65
PdmI GAANNNNTTC 1 cut(s) 339
PfeI GAWTC 2 cut(s) 262, 433
PkrI GCNGC 4 cut(s) 182, 325, 350, 396
PleI GAGTC 1 cut(s) 212
PpsI GAGTC 1 cut(s) 212
Psp124BI GAGCTC 1 cut(s) 127
Psp6I CCWGG 2 cut(s) 110, 255
PspFI CCCAGC 1 cut(s) 10
PspGI CCWGG 2 cut(s) 110, 255
PspN4I GGNNCC 1 cut(s) 288
PsuI RGATCY 1 cut(s) 27
RruI TCGCGA 1 cut(s) 267
RsaI GTAC 2 cut(s) 116, 288
RsaNI GTAC 2 cut(s) 115, 287
RseI CAYNNNNRTG 1 cut(s) 453
SacI GAGCTC 1 cut(s) 127
SalI GTCGAC 1 cut(s) 83
SatI GCNGC 4 cut(s) 181, 324, 349, 395
Sau3AI GATC 3 cut(s) 27, 93, 210
SchI GAGTC 1 cut(s) 212
ScrFI CCNGG 2 cut(s) 112, 257
SduI GDGCHC 1 cut(s) 127
SfcI CTRYAG 1 cut(s) 194
SmiMI CAYNNNNRTG 1 cut(s) 453
Sse9I AATT 2 cut(s) 172, 437
SsiI CCGC 1 cut(s) 181
SstI GAGCTC 1 cut(s) 127
StyD4I CCNGG 2 cut(s) 110, 255
StyI CCWWGG 1 cut(s) 206
TaiI ACGT 1 cut(s) 234
TaqI TCGA 4 cut(s) 24, 84, 92, 246
TasI AATT 2 cut(s) 172, 437
TauI GCSGC 1 cut(s) 183
TfiI GAWTC 2 cut(s) 262, 433
TscAI CASTG 2 cut(s) 466, 480
TseFI GTSAC 1 cut(s) 455
TseI GCWGC 3 cut(s) 323, 348, 394
Tsp45I GTSAC 1 cut(s) 455
TspGWI ACGGA 1 cut(s) 70
TspRI CASTG 2 cut(s) 466, 480
XapI RAATTY 1 cut(s) 172
XceI RCATGY 2 cut(s) 415, 470
XmiI GTMKAC 1 cut(s) 84
XmnI GAANNNNTTC 1 cut(s) 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.