RLG00000001252
MADS Family

MADS-box transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0003290
Physical Location & Seq
Forward (+)
12901258 .. 12922186
20929 bp
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UTR
Exon/CDS
Intron
RLM00000001252

Sequence Viewer

Length: 669 bp
ATGGGAAGGGGAAAGATTGTGATAAGGAGGATTGACAATTCAACGAGCAGGCAGGTGACGTTTTCGAAGCGGAGGAATGGATTGTTGAAGAAGGCCAGAGAACTGGCGATCCTGTGCGATGCTGAGGTCGGAGTCATGATCTTCTCCAGCACCGGCAAGCTCTACGATTTCGCAAGCACCAGCATAAAGTCAGTGATAGAAAGATATGGCAAAGCAAAAGAGGAGCATCATCAATTTGGCAACCCGGCTTCTGAAGTAAAGTTATGGCAAAGGGAAGCAGCAATGTTAAGGCAACAACTTCAAAACTTGCAAGAGAGCCATAGGCAAATGATGGGTGAAGAGCTTTCAGGCCTGAGTGTGAAAGACTTGCAGAATCTGGAGAACCAATTAGAAATGAGTCTTAGGGGTGTCCGAATGAAAAAGGATCAACTTTTAATGGATGAAATACAAGACCTAAACAGAAAGGGAAACCTCATTCACCAAGAAAATGTCGAGCTGTATAAGAAGGTTTATGGAACAAGGGATGTAAATGAAGCAAATAGAAATCCCTTTCTGACAAATGGTCCAGACATTGAAGAGGACACACATGGACCAGTCAATCTCCAGCTTAGTCAGCCACAGCAGCAAAACTATGAAGAACCAGCAAGAGCTACAAAGCTGGGACTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

223

Amino Acids

25.45

Weight (kDa)

8.9

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SRF-TF PF00319 10 - 57 2.1e-25 SRF-type transcription factor (DNA-binding and dimerisation domain)
K-box PF01486 84 - 170 1.1e-29 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 43
Acc36I ACCTGC 1 cut(s) 43
AciI CCGC 1 cut(s) 70
AclWI GGATC 2 cut(s) 103, 432
AcuI CTGAAG 1 cut(s) 273
AgsI TTSAA 4 cut(s) 42, 88, 302, 575
AhdI GACNNNNNGTC 1 cut(s) 561
AloI GAACNNNNNNTCC 2 cut(s) 93, 125
AluBI AGCT 6 cut(s) 160, 343, 496, 607, 650, 658
AluI AGCT 6 cut(s) 160, 343, 496, 607, 650, 658
AlwI GGATC 2 cut(s) 103, 432
AlwNI CAGNNNCTG 1 cut(s) 376
AoxI GGCC 2 cut(s) 93, 349
ApeKI GCWGC 2 cut(s) 278, 622
AspS9I GGNCC 2 cut(s) 563, 590
AsuC2I CCSGG 1 cut(s) 245
AsuHPI GGTGA 3 cut(s) 67, 347, 470
AsuII TTCGAA 1 cut(s) 65
AvaII GGWCC 2 cut(s) 563, 590
BbvCI CCTCAGC 1 cut(s) 123
BbvI GCAGC 2 cut(s) 290, 634
BccI CCATC 1 cut(s) 325
BcnI CCSGG 1 cut(s) 245
BfuAI ACCTGC 1 cut(s) 43
BisI GCNGC 2 cut(s) 279, 623
BlsI GCNGC 2 cut(s) 280, 624
Bme1390I CCNGG 1 cut(s) 245
Bme18I GGWCC 2 cut(s) 563, 590
BmeRI GACNNNNNGTC 1 cut(s) 561
BmgT120I GGNCC 2 cut(s) 563, 590
BmrFI CCNGG 1 cut(s) 245
BmsI GCATC 2 cut(s) 109, 235
BpmI CTGGAG 3 cut(s) 130, 398, 587
Bpu10I CCTNAGC 1 cut(s) 123
Bpu14I TTCGAA 1 cut(s) 65
BpuMI CCSGG 1 cut(s) 245
Bse118I RCCGGY 1 cut(s) 152
Bse1I ACTGG 2 cut(s) 108, 593
Bse3DI GCAATG 1 cut(s) 288
BseGI GGATG 2 cut(s) 445, 529
BseMI GCAATG 1 cut(s) 288
BseMII CTCAG 2 cut(s) 114, 344
BseNI ACTGG 2 cut(s) 108, 593
BseRI GAGGAG 1 cut(s) 236
BseXI GCAGC 2 cut(s) 290, 634
BseYI CCCAGC 1 cut(s) 658
BshFI GGCC 2 cut(s) 95, 351
BsiSI CCGG 2 cut(s) 153, 245
BsnI GGCC 2 cut(s) 95, 351
Bsp119I TTCGAA 1 cut(s) 65
Bsp143I GATC 3 cut(s) 108, 138, 424
BspACI CCGC 1 cut(s) 70
BspANI GGCC 2 cut(s) 95, 351
BspCNI CTCAG 2 cut(s) 115, 345
BspHI TCATGA 1 cut(s) 135
BspMI ACCTGC 1 cut(s) 43
BspPI GGATC 2 cut(s) 103, 432
BspQI GCTCTTC 1 cut(s) 333
BspT104I TTCGAA 1 cut(s) 65
BsrDI GCAATG 1 cut(s) 288
BsrFI RCCGGY 1 cut(s) 152
BsrI ACTGG 2 cut(s) 108, 593
BssAI RCCGGY 1 cut(s) 152
BssMI GATC 3 cut(s) 108, 138, 424
Bst6I CTCTTC 2 cut(s) 333, 570
BstBI TTCGAA 1 cut(s) 65
BstC8I GCNNGC 3 cut(s) 50, 158, 175
BstDEI CTNAG 4 cut(s) 123, 353, 401, 608
BstF5I GGATG 2 cut(s) 445, 529
BstKTI GATC 3 cut(s) 111, 141, 427
BstMBI GATC 3 cut(s) 108, 138, 424
BstMWI GCNNNNNNNGC 2 cut(s) 613, 622
BstSCI CCNGG 1 cut(s) 243
BstV1I GCAGC 2 cut(s) 290, 634
BstXI CCANNNNNNTGG 1 cut(s) 103
BsuRI GGCC 2 cut(s) 95, 351
BtgZI GCGATG 1 cut(s) 132
BtsCI GGATG 2 cut(s) 445, 529
BtsIMutI CAGTG 1 cut(s) 198
BveI ACCTGC 1 cut(s) 43
Cac8I GCNNGC 3 cut(s) 50, 158, 175
CaiI CAGNNNCTG 1 cut(s) 376
CciI TCATGA 1 cut(s) 135
Cfr10I RCCGGY 1 cut(s) 152
Cfr13I GGNCC 2 cut(s) 563, 590
CviAII CATG 2 cut(s) 136, 587
DdeI CTNAG 4 cut(s) 123, 353, 401, 608
DpnI GATC 3 cut(s) 110, 140, 426
DpnII GATC 3 cut(s) 108, 138, 424
DriI GACNNNNNGTC 1 cut(s) 561
Eam1104I CTCTTC 2 cut(s) 333, 570
Eam1105I GACNNNNNGTC 1 cut(s) 561
EarI CTCTTC 2 cut(s) 333, 570
Eco147I AGGCCT 1 cut(s) 351
Eco47I GGWCC 2 cut(s) 563, 590
Eco57I CTGAAG 1 cut(s) 273
FaeI CATG 2 cut(s) 139, 590
FaiI YATR 9 cut(s) 137, 185, 207, 265, 321, 501, 513, 588, 633
FatI CATG 2 cut(s) 135, 586
Fnu4HI GCNGC 2 cut(s) 279, 623
FokI GGATG 2 cut(s) 452, 536
Fsp4HI GCNGC 2 cut(s) 279, 623
GluI GCNGC 2 cut(s) 279, 623
GsaI CCCAGC 1 cut(s) 662
GsuI CTGGAG 3 cut(s) 130, 398, 587
HaeIII GGCC 2 cut(s) 95, 351
HapII CCGG 2 cut(s) 153, 245
Hin1II CATG 2 cut(s) 139, 590
HinfI GANTC 3 cut(s) 132, 373, 397
HpaII CCGG 2 cut(s) 153, 245
HphI GGTGA 3 cut(s) 67, 347, 470
Hpy188I TCNGA 4 cut(s) 131, 253, 413, 555
Hpy188III TCNNGA 3 cut(s) 136, 377, 566
HpyAV CCTTC 2 cut(s) 85, 499
HpyCH4IV ACGT 1 cut(s) 59
HpyCH4V TGCA 2 cut(s) 310, 370
HpyF10VI GCNNNNNNNGC 2 cut(s) 613, 622
HpyF3I CTNAG 4 cut(s) 123, 353, 401, 608
HpySE526I ACGT 1 cut(s) 59
Hsp92II CATG 2 cut(s) 139, 590
Kzo9I GATC 3 cut(s) 108, 138, 424
LguI GCTCTTC 1 cut(s) 333
LmnI GCTCC 1 cut(s) 223
Lsp1109I GCAGC 2 cut(s) 290, 634
LweI GCATC 2 cut(s) 109, 235
MaeII ACGT 1 cut(s) 59
MaeIII GTNAC 1 cut(s) 55
MalI GATC 3 cut(s) 110, 140, 426
MboI GATC 3 cut(s) 108, 138, 424
MboII GAAGA 5 cut(s) 100, 133, 350, 587, 647
MluCI AATT 3 cut(s) 37, 233, 386
MlyI GAGTC 2 cut(s) 141, 406
MmeI TCCRAC 1 cut(s) 109
MnlI CCTC 6 cut(s) 21, 66, 118, 214, 482, 571
MseI TTAA 2 cut(s) 287, 434
MspI CCGG 2 cut(s) 153, 245
MspR9I CCNGG 1 cut(s) 245
MwoI GCNNNNNNNGC 2 cut(s) 613, 622
NciI CCSGG 1 cut(s) 245
NdeII GATC 3 cut(s) 108, 138, 424
NlaIII CATG 2 cut(s) 139, 590
NmuCI GTSAC 1 cut(s) 55
NspV TTCGAA 1 cut(s) 65
PagI TCATGA 1 cut(s) 135
PaqCI CACCTGC 1 cut(s) 43
PceI AGGCCT 1 cut(s) 351
PciSI GCTCTTC 1 cut(s) 333
PfeI GAWTC 1 cut(s) 373
PkrI GCNGC 2 cut(s) 280, 624
PleI GAGTC 2 cut(s) 140, 405
PpsI GAGTC 2 cut(s) 140, 405
PspFI CCCAGC 1 cut(s) 658
PspPI GGNCC 2 cut(s) 563, 590
PstNI CAGNNNCTG 1 cut(s) 376
SapI GCTCTTC 1 cut(s) 333
SaqAI TTAA 2 cut(s) 287, 434
SatI GCNGC 2 cut(s) 279, 623
Sau3AI GATC 3 cut(s) 108, 138, 424
Sau96I GGNCC 2 cut(s) 563, 590
SchI GAGTC 2 cut(s) 141, 406
ScrFI CCNGG 1 cut(s) 245
SfaNI GCATC 2 cut(s) 109, 235
SfuI TTCGAA 1 cut(s) 65
SinI GGWCC 2 cut(s) 563, 590
Sse9I AATT 3 cut(s) 37, 233, 386
SseBI AGGCCT 1 cut(s) 351
SsiI CCGC 1 cut(s) 70
StuI AGGCCT 1 cut(s) 351
StyD4I CCNGG 1 cut(s) 243
TaiI ACGT 1 cut(s) 62
TaqI TCGA 2 cut(s) 65, 492
TasI AATT 3 cut(s) 37, 233, 386
TfiI GAWTC 1 cut(s) 373
Tru1I TTAA 2 cut(s) 287, 434
Tru9I TTAA 2 cut(s) 287, 434
TscAI CASTG 1 cut(s) 198
TseFI GTSAC 1 cut(s) 55
TseI GCWGC 2 cut(s) 278, 622
Tsp45I GTSAC 1 cut(s) 55
TspDTI ATGAA 4 cut(s) 431, 456, 546, 648
TspRI CASTG 1 cut(s) 198
VpaK11BI GGWCC 2 cut(s) 563, 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.