Rroxscaffold_3G00227200
MADS Family

MADS-box transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0003290
Physical Location & Seq
Forward (+)
11267737 .. 11273379
5643 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00227200.1

Sequence Viewer

Length: 1035 bp
ATGTTAAGGCAACAACTTCAAAACTTGCAAGAGAGCCATAGGCAAATGATGGGTGAAGAGCTTTCAGGCCTGAGTGTGAAAGACTTGCAGAATCTGGAGAACCAATTGGAAATGAGTCTTAGGGGTGTCCGAATGAAAAAGGATCAACTTTTAATGGATGAAATACAAGACCTAAACAGAAAGGGAAACCTCATTCACCAAGAAAATGTCGAGCTGTATAAGAAGGTAAACCTAATTCGTCAAGAAAACATGGAACTATATAAGAAGGTTTATGGAACAAGGGATGTACATGGAGCAAACAGAAATCCCTTTCTGACAAATGGTCTAGACATTGGAGAGGACACACATGGACCAGTCAATCTCCAGCTTAGTCAGCCACAGCAGCAGAACCATGAAGAACCAGCAAGAGCTACAAAGCTGGGTTGTCAGTACTCAGGGTCAGATTCCAGCACAGTTCTGTGCTATGTTGAGCAGTACTTCAGCGAAAGCGCAACACTACCTAACCAATCTGCACAATGGCAGCTACCATTGCTGATCAAATGCGTCCACAAGCTAGCCGGTGGTTCTTTTCTCTTTTCTTACGATCTGCTTCCTGGTCCTACACATGGCATCACCCAACCAAGGGTCCCCTATTGCTGCAAGAATTCAGAGCAGCTGGATAAGGGAAAGTCTGTTGTCATTGACACCACTCCTGCTATGGCAGATGATGGTGAGGGTCCATCTTTTGACCGACAATCTCCTCCTCCAATTGAAAAGGCTGCACTGAATACCTCTAAGTCGATACCAAATATTCGTATTGAAAAAGCTGCAGTGAATAACTCTTTTTTTAAGTCAATACCAAATGTCGTTGTTGAGCAAGCCATTGTTCCAATAGTACAAGAGCAAGGCAAGAGCATTGTTGCAGATGAGCAACAGATAGTTGGAGAGCGCATTGACACTTTGGTTGAGATAAGTCCTTTGAGTTCCTGGTTTGATGAGGGTGAAACAACTCTGGATTGTGAAGATTCTCAAGTCTCCCCCAAAGTCTTAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
K-box SRF-TF

Protein Analysis

344

Amino Acids

38.5

Weight (kDa)

5.05

Isoelectric Point (pI)

45.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
K-box PF01486 2 - 76 7.2e-25 K-box region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 150
AcsI RAATTY 1 cut(s) 643
AcuI CTGAAG 1 cut(s) 463
AfaI GTAC 4 cut(s) 288, 431, 476, 876
AfiI CCNNNNNNNGG 3 cut(s) 605, 621, 622
AgsI TTSAA 3 cut(s) 20, 752, 800
AhdI GACNNNNNGTC 1 cut(s) 321
AjnI CCWGG 2 cut(s) 592, 965
AluBI AGCT 9 cut(s) 61, 214, 367, 410, 418, 523, 553, 655, 806
AluI AGCT 9 cut(s) 61, 214, 367, 410, 418, 523, 553, 655, 806
Alw26I GTCTC 1 cut(s) 1018
AlwI GGATC 1 cut(s) 150
AlwNI CAGNNNCTG 1 cut(s) 94
AoxI GGCC 1 cut(s) 67
ApeKI GCWGC 6 cut(s) 382, 520, 636, 652, 758, 806
ApoI RAATTY 1 cut(s) 643
AspLEI GCGC 2 cut(s) 491, 930
AspS9I GGNCC 4 cut(s) 350, 596, 625, 716
AsuHPI GGTGA 5 cut(s) 65, 188, 604, 722, 992
AsuNHI GCTAGC 1 cut(s) 553
AvaII GGWCC 4 cut(s) 350, 596, 625, 716
BbvI GCAGC 6 cut(s) 394, 532, 623, 664, 745, 793
BccI CCATC 3 cut(s) 43, 701, 727
BciT130I CCWGG 2 cut(s) 594, 967
BclI TGATCA 1 cut(s) 534
BcoDI GTCTC 1 cut(s) 1018
BfaI CTAG 2 cut(s) 326, 554
BfmI CTRYAG 1 cut(s) 807
BisI GCNGC 6 cut(s) 383, 521, 637, 653, 759, 807
BlsI GCNGC 6 cut(s) 384, 522, 638, 654, 760, 808
BmcAI AGTACT 2 cut(s) 431, 476
Bme1390I CCNGG 2 cut(s) 594, 967
Bme18I GGWCC 4 cut(s) 350, 596, 625, 716
BmeRI GACNNNNNGTC 1 cut(s) 321
BmgT120I GGNCC 4 cut(s) 350, 596, 625, 716
BmiI GGNNCC 3 cut(s) 626, 627, 717
BmrFI CCNGG 2 cut(s) 594, 967
BmsI GCATC 1 cut(s) 618
BmtI GCTAGC 1 cut(s) 557
BpmI CTGGAG 2 cut(s) 116, 347
BpuEI CTTGAG 1 cut(s) 993
BsaJI CCNNGG 1 cut(s) 620
Bsc4I CCNNNNNNNGG 3 cut(s) 605, 621, 622
Bse118I RCCGGY 1 cut(s) 557
Bse1I ACTGG 1 cut(s) 353
Bse3DI GCAATG 1 cut(s) 527
BseBI CCWGG 2 cut(s) 594, 967
BseDI CCNNGG 1 cut(s) 620
BseGI GGATG 2 cut(s) 163, 289
BseLI CCNNNNNNNGG 3 cut(s) 605, 621, 622
BseMI GCAATG 1 cut(s) 527
BseMII CTCAG 2 cut(s) 62, 447
BseNI ACTGG 1 cut(s) 353
BseRI GAGGAG 2 cut(s) 729, 732
BseXI GCAGC 6 cut(s) 394, 532, 623, 664, 745, 793
BseYI CCCAGC 1 cut(s) 418
BsgI GTGCAG 2 cut(s) 495, 744
BshFI GGCC 1 cut(s) 69
BsiSI CCGG 1 cut(s) 558
BslFI GGGAC 1 cut(s) 611
BslI CCNNNNNNNGG 3 cut(s) 605, 621, 622
BsmAI GTCTC 1 cut(s) 1018
BsmFI GGGAC 1 cut(s) 611
BsnI GGCC 1 cut(s) 69
Bsp1407I TGTACA 1 cut(s) 286
Bsp143I GATC 3 cut(s) 142, 534, 583
BspANI GGCC 1 cut(s) 69
BspCNI CTCAG 2 cut(s) 63, 446
BspLI GGNNCC 3 cut(s) 626, 627, 717
BspMAI CTGCAG 1 cut(s) 811
BspOI GCTAGC 1 cut(s) 557
BspPI GGATC 1 cut(s) 150
BspQI GCTCTTC 1 cut(s) 51
BsrDI GCAATG 1 cut(s) 527
BsrFI RCCGGY 1 cut(s) 557
BsrGI TGTACA 1 cut(s) 286
BsrI ACTGG 1 cut(s) 353
BssAI RCCGGY 1 cut(s) 557
BssECI CCNNGG 1 cut(s) 620
BssMI GATC 3 cut(s) 142, 534, 583
BssT1I CCWWGG 1 cut(s) 620
Bst2UI CCWGG 2 cut(s) 594, 967
Bst4CI ACNGT 1 cut(s) 454
Bst6I CTCTTC 1 cut(s) 51
BstAUI TGTACA 1 cut(s) 286
BstC8I GCNNGC 2 cut(s) 555, 858
BstDEI CTNAG 6 cut(s) 71, 119, 368, 433, 774, 1027
BstF5I GGATG 2 cut(s) 163, 289
BstHHI GCGC 2 cut(s) 491, 930
BstKTI GATC 3 cut(s) 145, 537, 586
BstMAI GTCTC 1 cut(s) 1018
BstMBI GATC 3 cut(s) 142, 534, 583
BstMWI GCNNNNNNNGC 3 cut(s) 373, 382, 529
BstNI CCWGG 2 cut(s) 594, 967
BstSCI CCNGG 2 cut(s) 592, 965
BstSFI CTRYAG 1 cut(s) 807
BstV1I GCAGC 6 cut(s) 394, 532, 623, 664, 745, 793
BsuRI GGCC 1 cut(s) 69
BtsCI GGATG 2 cut(s) 163, 289
BtsI GCAGTG 1 cut(s) 816
BtsIMutI CAGTG 2 cut(s) 761, 816
Cac8I GCNNGC 2 cut(s) 555, 858
CaiI CAGNNNCTG 1 cut(s) 94
CfoI GCGC 2 cut(s) 491, 930
Cfr10I RCCGGY 1 cut(s) 557
Cfr13I GGNCC 4 cut(s) 350, 596, 625, 716
CseI GACGC 1 cut(s) 532
Csp6I GTAC 4 cut(s) 287, 430, 475, 875
CviAII CATG 5 cut(s) 250, 290, 347, 392, 605
CviQI GTAC 4 cut(s) 287, 430, 475, 875
DdeI CTNAG 6 cut(s) 71, 119, 368, 433, 774, 1027
DpnI GATC 3 cut(s) 144, 536, 585
DpnII GATC 3 cut(s) 142, 534, 583
DriI GACNNNNNGTC 1 cut(s) 321
Eam1104I CTCTTC 1 cut(s) 51
Eam1105I GACNNNNNGTC 1 cut(s) 321
EarI CTCTTC 1 cut(s) 51
Eco130I CCWWGG 1 cut(s) 620
Eco147I AGGCCT 1 cut(s) 69
Eco47I GGWCC 4 cut(s) 350, 596, 625, 716
Eco57I CTGAAG 1 cut(s) 463
EcoO109I RGGNCCY 1 cut(s) 625
EcoRI GAATTC 1 cut(s) 643
EcoRII CCWGG 2 cut(s) 592, 965
EcoT14I CCWWGG 1 cut(s) 620
ErhI CCWWGG 1 cut(s) 620
FaeI CATG 5 cut(s) 253, 293, 350, 395, 608
FaqI GGGAC 1 cut(s) 611
FatI CATG 5 cut(s) 249, 289, 346, 391, 604
FbaI TGATCA 1 cut(s) 534
Fnu4HI GCNGC 6 cut(s) 383, 521, 637, 653, 759, 807
FokI GGATG 2 cut(s) 170, 296
Fsp4HI GCNGC 6 cut(s) 383, 521, 637, 653, 759, 807
FspBI CTAG 2 cut(s) 326, 554
GlaI GCGC 2 cut(s) 490, 929
GluI GCNGC 6 cut(s) 383, 521, 637, 653, 759, 807
GsaI CCCAGC 1 cut(s) 422
GsuI CTGGAG 2 cut(s) 116, 347
HaeIII GGCC 1 cut(s) 69
HapII CCGG 1 cut(s) 558
HgaI GACGC 1 cut(s) 532
HhaI GCGC 2 cut(s) 491, 930
Hin1II CATG 5 cut(s) 253, 293, 350, 395, 608
Hin6I GCGC 2 cut(s) 489, 928
HinP1I GCGC 2 cut(s) 489, 928
HinfI GANTC 4 cut(s) 91, 115, 443, 1004
HpaII CCGG 1 cut(s) 558
HphI GGTGA 5 cut(s) 65, 188, 604, 722, 992
Hpy166II GTNNAC 2 cut(s) 229, 547
Hpy188I TCNGA 4 cut(s) 131, 315, 442, 649
Hpy188III TCNNGA 4 cut(s) 95, 242, 326, 992
Hpy8I GTNNAC 2 cut(s) 229, 547
HpyAV CCTTC 2 cut(s) 217, 259
HpyCH4III ACNGT 1 cut(s) 454
HpyCH4V TGCA 7 cut(s) 28, 88, 512, 639, 761, 809, 902
HpyF10VI GCNNNNNNNGC 3 cut(s) 373, 382, 529
HpyF3I CTNAG 6 cut(s) 71, 119, 368, 433, 774, 1027
Hsp92II CATG 5 cut(s) 253, 293, 350, 395, 608
HspAI GCGC 2 cut(s) 489, 928
KflI GGGWCCC 1 cut(s) 625
Ksp22I TGATCA 1 cut(s) 534
Kzo9I GATC 3 cut(s) 142, 534, 583
LguI GCTCTTC 1 cut(s) 51
LmnI GCTCC 1 cut(s) 293
Lsp1109I GCAGC 6 cut(s) 394, 532, 623, 664, 745, 793
LweI GCATC 1 cut(s) 618
MaeI CTAG 2 cut(s) 326, 554
MalI GATC 3 cut(s) 144, 536, 585
MboI GATC 3 cut(s) 142, 534, 583
MboII GAAGA 3 cut(s) 68, 407, 1013
MfeI CAATTG 2 cut(s) 104, 747
MluCI AATT 4 cut(s) 104, 234, 643, 747
MlyI GAGTC 1 cut(s) 124
MmeI TCCRAC 1 cut(s) 901
MnlI CCTC 7 cut(s) 200, 331, 706, 750, 753, 781, 970
MseI TTAA 3 cut(s) 5, 152, 828
MspA1I CMGCKG 1 cut(s) 655
MspI CCGG 1 cut(s) 558
MspR9I CCNGG 2 cut(s) 594, 967
MunI CAATTG 2 cut(s) 104, 747
MvaI CCWGG 2 cut(s) 594, 967
MwoI GCNNNNNNNGC 3 cut(s) 373, 382, 529
NdeII GATC 3 cut(s) 142, 534, 583
NheI GCTAGC 1 cut(s) 553
NlaIII CATG 5 cut(s) 253, 293, 350, 395, 608
NlaIV GGNNCC 3 cut(s) 626, 627, 717
PceI AGGCCT 1 cut(s) 69
PciSI GCTCTTC 1 cut(s) 51
PfeI GAWTC 3 cut(s) 91, 443, 1004
PkrI GCNGC 6 cut(s) 384, 522, 638, 654, 760, 808
PleI GAGTC 1 cut(s) 123
PpsI GAGTC 1 cut(s) 123
PpuMI RGGWCCY 1 cut(s) 625
Psp5II RGGWCCY 1 cut(s) 625
Psp6I CCWGG 2 cut(s) 592, 965
PspFI CCCAGC 1 cut(s) 418
PspGI CCWGG 2 cut(s) 592, 965
PspN4I GGNNCC 3 cut(s) 626, 627, 717
PspPI GGNCC 4 cut(s) 350, 596, 625, 716
PspPPI RGGWCCY 1 cut(s) 625
PstI CTGCAG 1 cut(s) 811
PstNI CAGNNNCTG 1 cut(s) 94
PvuII CAGCTG 1 cut(s) 655
RsaI GTAC 4 cut(s) 288, 431, 476, 876
RsaNI GTAC 4 cut(s) 287, 430, 475, 875
SapI GCTCTTC 1 cut(s) 51
SaqAI TTAA 3 cut(s) 5, 152, 828
SatI GCNGC 6 cut(s) 383, 521, 637, 653, 759, 807
Sau3AI GATC 3 cut(s) 142, 534, 583
Sau96I GGNCC 4 cut(s) 350, 596, 625, 716
ScaI AGTACT 2 cut(s) 431, 476
SchI GAGTC 1 cut(s) 124
ScrFI CCNGG 2 cut(s) 594, 967
SfaNI GCATC 1 cut(s) 618
SfcI CTRYAG 1 cut(s) 807
SinI GGWCC 4 cut(s) 350, 596, 625, 716
SmlI CTYRAG 1 cut(s) 1008
SmoI CTYRAG 1 cut(s) 1008
Sse9I AATT 4 cut(s) 104, 234, 643, 747
SseBI AGGCCT 1 cut(s) 69
SspI AATATT 1 cut(s) 790
SspMI CTAG 2 cut(s) 326, 554
StuI AGGCCT 1 cut(s) 69
StyD4I CCNGG 2 cut(s) 592, 965
StyI CCWWGG 1 cut(s) 620
TaaI ACNGT 1 cut(s) 454
TaqI TCGA 2 cut(s) 210, 779
TaqII GACCGA 1 cut(s) 744
TasI AATT 4 cut(s) 104, 234, 643, 747
TatI WGTACW 4 cut(s) 286, 429, 474, 874
TfiI GAWTC 3 cut(s) 91, 443, 1004
Tru1I TTAA 3 cut(s) 5, 152, 828
Tru9I TTAA 3 cut(s) 5, 152, 828
TscAI CASTG 2 cut(s) 768, 816
TseI GCWGC 6 cut(s) 382, 520, 636, 652, 758, 806
TspDTI ATGAA 3 cut(s) 149, 174, 408
TspRI CASTG 2 cut(s) 768, 816
VpaK11BI GGWCC 4 cut(s) 350, 596, 625, 716
XapI RAATTY 1 cut(s) 643
XbaI TCTAGA 1 cut(s) 325
XcmI CCANNNNNNNNNTGG 1 cut(s) 694
XspI CTAG 2 cut(s) 326, 554
ZrmI AGTACT 2 cut(s) 431, 476
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.