RLG00000020608

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0005194
Physical Location & Seq
Reverse (-)
66700013 .. 66702984
2972 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020608

Sequence Viewer

Length: 690 bp
ATGAGTTCCTGGAAATCAAAAATGAGTATAAGTAGCAGCAGCTGCAATTGTTCTTCATCAGCCAAAATCCGGAAAGGCTATGTTCCGATGATTATTGTGGCGAAACAAGGGGATGATGATCATGATCAGAAAGTATGGATACGAATTAAGCTAATGAACCATCCTCGAATTGTTGCACTACTCGAAGACCAAGCAGATGAATTTGGGTTTCACCAACAAGGCTTGCTTAAGATTAGATACGACCACCATCTTTTTAAAGGCATGGCCGCAGATTTCCCGGACACTAATACTTTGAAGTATGCTCTTGGTGTCAATGAGTTTGAAGATTTTGAAGTGACAGAAGTTGATGGAACCATTCTGTGGGACTTGCTTGAAGAGTTGGAGAATGAAGAGAAGAAGGGTGACAACAGTGAGCTCATTGGTCATGAACCTAATAGTATGATGACCATTGCCGAGGTTTCGTTGGACAAGAACGAGATCTCCATTCCTGACTCAAACTCAAATTGGGTAGCGATGGATGATATGATGATGGAGACTGATCCGTTCCATGAAATAGAGGGTTGGTTCACAGATGACATGGTGGGAATGGTGGACTTTGGTTATAGTGTTGGTGATTACTCACAATTGCACAATGGGGTTGGAGCCTCCAGCAATGAAATTACTTACAATTGCTTGTGGGAAGAGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

230

Amino Acids

26.01

Weight (kDa)

4.39

Isoelectric Point (pI)

33.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Auxin_inducible PF02519 5 - 86 2e-08 Auxin responsive protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 360
AccIII TCCGGA 1 cut(s) 69
AciI CCGC 1 cut(s) 267
AclWI GGATC 1 cut(s) 533
AcoI YGGCCR 1 cut(s) 264
AcsI RAATTY 1 cut(s) 200
AfiI CCNNNNNNNGG 2 cut(s) 69, 360
AflII CTTAAG 1 cut(s) 227
AgsI TTSAA 4 cut(s) 295, 323, 332, 374
AjnI CCWGG 1 cut(s) 8
AloI GAACNNNNNNTCC 2 cut(s) 464, 496
AluBI AGCT 4 cut(s) 42, 151, 415, 687
AluI AGCT 4 cut(s) 42, 151, 415, 687
Alw21I GWGCWC 1 cut(s) 417
Alw26I GTCTC 1 cut(s) 527
AlwI GGATC 1 cut(s) 533
AlwNI CAGNNNCTG 1 cut(s) 42
Aor13HI TCCGGA 1 cut(s) 69
AoxI GGCC 1 cut(s) 264
ApeKI GCWGC 3 cut(s) 36, 39, 42
ApoI RAATTY 1 cut(s) 200
AsuC2I CCSGG 1 cut(s) 278
AsuHPI GGTGA 3 cut(s) 203, 413, 623
BanII GRGCYC 1 cut(s) 417
BbsI GAAGAC 1 cut(s) 192
Bbv12I GWGCWC 1 cut(s) 417
BbvI GCAGC 3 cut(s) 29, 48, 51
BccI CCATC 5 cut(s) 168, 255, 341, 508, 523
BciT130I CCWGG 1 cut(s) 10
BciVI GTATCC 1 cut(s) 132
BclI TGATCA 2 cut(s) 118, 124
BcnI CCSGG 1 cut(s) 278
BcoDI GTCTC 1 cut(s) 527
BfrI CTTAAG 1 cut(s) 227
BfuI GTATCC 1 cut(s) 132
BglII AGATCT 1 cut(s) 477
BisI GCNGC 4 cut(s) 37, 40, 43, 267
BlsI GCNGC 4 cut(s) 38, 41, 44, 268
Bme1390I CCNGG 2 cut(s) 10, 278
BmiI GGNNCC 2 cut(s) 352, 643
BmrFI CCNGG 2 cut(s) 10, 278
BpiI GAAGAC 1 cut(s) 192
BpmI CTGGAG 1 cut(s) 631
BpuMI CCSGG 1 cut(s) 278
BsaBI GATNNNNATC 2 cut(s) 117, 123
BsaJI CCNNGG 1 cut(s) 453
BsaWI WCCGGW 1 cut(s) 69
BsaXI ACNNNNNCTCC 2 cut(s) 464, 494
Bsc4I CCNNNNNNNGG 2 cut(s) 69, 360
Bse3DI GCAATG 2 cut(s) 447, 658
Bse8I GATNNNNATC 2 cut(s) 117, 123
BseAI TCCGGA 1 cut(s) 69
BseBI CCWGG 1 cut(s) 10
BseDI CCNNGG 1 cut(s) 453
BseGI GGATG 3 cut(s) 118, 160, 523
BseJI GATNNNNATC 2 cut(s) 117, 123
BseLI CCNNNNNNNGG 2 cut(s) 69, 360
BseMI GCAATG 2 cut(s) 447, 658
BseXI GCAGC 3 cut(s) 29, 48, 51
BshFI GGCC 1 cut(s) 266
BsiHKAI GWGCWC 1 cut(s) 417
BsiSI CCGG 2 cut(s) 70, 278
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 2 cut(s) 69, 360
BsmAI GTCTC 1 cut(s) 527
BsmFI GGGAC 1 cut(s) 377
BsnI GGCC 1 cut(s) 266
Bsp1286I GDGCHC 1 cut(s) 417
Bsp13I TCCGGA 1 cut(s) 69
Bsp143I GATC 4 cut(s) 118, 124, 477, 538
BspACI CCGC 1 cut(s) 267
BspANI GGCC 1 cut(s) 266
BspEI TCCGGA 1 cut(s) 69
BspHI TCATGA 2 cut(s) 121, 424
BspLI GGNNCC 2 cut(s) 352, 643
BspPI GGATC 1 cut(s) 533
BspTI CTTAAG 1 cut(s) 227
BsrDI GCAATG 2 cut(s) 447, 658
BssECI CCNNGG 1 cut(s) 453
BssMI GATC 4 cut(s) 118, 124, 477, 538
Bst2UI CCWGG 1 cut(s) 10
Bst4CI ACNGT 1 cut(s) 410
Bst6I CTCTTC 3 cut(s) 369, 384, 675
BstAFI CTTAAG 1 cut(s) 227
BstAPI GCANNNNNTGC 1 cut(s) 42
BstC8I GCNNGC 1 cut(s) 224
BstF5I GGATG 3 cut(s) 118, 160, 523
BstKTI GATC 4 cut(s) 121, 127, 480, 541
BstMAI GTCTC 1 cut(s) 527
BstMBI GATC 4 cut(s) 118, 124, 477, 538
BstMWI GCNNNNNNNGC 1 cut(s) 42
BstNI CCWGG 1 cut(s) 10
BstSCI CCNGG 2 cut(s) 8, 276
BstV1I GCAGC 3 cut(s) 29, 48, 51
BstV2I GAAGAC 1 cut(s) 192
BstX2I RGATCY 1 cut(s) 477
BstYI RGATCY 1 cut(s) 477
BsuI GTATCC 1 cut(s) 132
BsuRI GGCC 1 cut(s) 266
BtgZI GCGATG 1 cut(s) 527
BtsCI GGATG 3 cut(s) 118, 160, 523
BtsIMutI CAGTG 1 cut(s) 415
Cac8I GCNNGC 1 cut(s) 224
CaiI CAGNNNCTG 1 cut(s) 42
CciI TCATGA 2 cut(s) 121, 424
CviAII CATG 5 cut(s) 122, 262, 425, 548, 577
CviJI RGCY 9 cut(s) 42, 62, 78, 151, 222, 266, 415, 644, 687
CviKI_1 RGCY 9 cut(s) 42, 62, 78, 151, 222, 266, 415, 644, 687
DpnI GATC 4 cut(s) 120, 126, 479, 540
DpnII GATC 4 cut(s) 118, 124, 477, 538
DraI TTTAAA 1 cut(s) 256
EaeI YGGCCR 1 cut(s) 264
Eam1104I CTCTTC 3 cut(s) 369, 384, 675
EarI CTCTTC 3 cut(s) 369, 384, 675
Ecl136II GAGCTC 1 cut(s) 415
Eco24I GRGCYC 1 cut(s) 417
Eco53kI GAGCTC 1 cut(s) 415
EcoICRI GAGCTC 1 cut(s) 415
EcoRII CCWGG 1 cut(s) 8
EcoT38I GRGCYC 1 cut(s) 417
FaeI CATG 5 cut(s) 125, 265, 428, 551, 580
FalI AAGNNNNNCTT 2 cut(s) 210, 242
FaqI GGGAC 1 cut(s) 377
FatI CATG 5 cut(s) 121, 261, 424, 547, 576
FbaI TGATCA 2 cut(s) 118, 124
Fnu4HI GCNGC 4 cut(s) 37, 40, 43, 267
FokI GGATG 3 cut(s) 125, 147, 530
FriOI GRGCYC 1 cut(s) 417
Fsp4HI GCNGC 4 cut(s) 37, 40, 43, 267
GluI GCNGC 4 cut(s) 37, 40, 43, 267
GsuI CTGGAG 1 cut(s) 631
HaeIII GGCC 1 cut(s) 266
HapII CCGG 2 cut(s) 70, 278
Hin1II CATG 5 cut(s) 125, 265, 428, 551, 580
HinfI GANTC 1 cut(s) 491
HpaII CCGG 2 cut(s) 70, 278
HphI GGTGA 3 cut(s) 203, 413, 623
Hpy166II GTNNAC 2 cut(s) 567, 592
Hpy188I TCNGA 2 cut(s) 87, 129
Hpy188III TCNNGA 4 cut(s) 70, 122, 425, 488
Hpy8I GTNNAC 2 cut(s) 567, 592
HpyAV CCTTC 1 cut(s) 391
HpyCH4III ACNGT 1 cut(s) 410
HpyCH4V TGCA 3 cut(s) 45, 176, 628
HpyF10VI GCNNNNNNNGC 1 cut(s) 42
Hsp92II CATG 5 cut(s) 125, 265, 428, 551, 580
Kpn2I TCCGGA 1 cut(s) 69
Ksp22I TGATCA 2 cut(s) 118, 124
Kzo9I GATC 4 cut(s) 118, 124, 477, 538
LmnI GCTCC 1 cut(s) 641
LpnPI CCDG 5 cut(s) 22, 83, 291, 501, 661
Lsp1109I GCAGC 3 cut(s) 29, 48, 51
MaeIII GTNAC 2 cut(s) 334, 401
MalI GATC 4 cut(s) 120, 126, 479, 540
MboI GATC 4 cut(s) 118, 124, 477, 538
MboII GAAGA 6 cut(s) 45, 197, 335, 386, 401, 406
MfeI CAATTG 3 cut(s) 46, 623, 667
MflI RGATCY 1 cut(s) 477
MhlI GDGCHC 1 cut(s) 417
MluCI AATT 8 cut(s) 46, 144, 168, 200, 502, 623, 657, 667
MlyI GAGTC 1 cut(s) 485
MmeI TCCRAC 3 cut(s) 360, 444, 619
MnlI CCTC 4 cut(s) 174, 448, 550, 655
MroI TCCGGA 1 cut(s) 69
MseI TTAA 3 cut(s) 147, 228, 255
MspA1I CMGCKG 1 cut(s) 42
MspCI CTTAAG 1 cut(s) 227
MspI CCGG 2 cut(s) 70, 278
MspR9I CCNGG 2 cut(s) 10, 278
MunI CAATTG 3 cut(s) 46, 623, 667
MvaI CCWGG 1 cut(s) 10
MwoI GCNNNNNNNGC 1 cut(s) 42
NciI CCSGG 1 cut(s) 278
NdeII GATC 4 cut(s) 118, 124, 477, 538
NlaIII CATG 5 cut(s) 125, 265, 428, 551, 580
NlaIV GGNNCC 2 cut(s) 352, 643
NmeAIII GCCGAG 1 cut(s) 478
NmuCI GTSAC 2 cut(s) 334, 401
PagI TCATGA 2 cut(s) 121, 424
PflMI CCANNNNNTGG 1 cut(s) 360
PfoI TCCNGGA 2 cut(s) 8, 276
PkrI GCNGC 4 cut(s) 38, 41, 44, 268
PleI GAGTC 1 cut(s) 485
PpsI GAGTC 1 cut(s) 485
Psp124BI GAGCTC 1 cut(s) 417
Psp6I CCWGG 1 cut(s) 8
PspGI CCWGG 1 cut(s) 8
PspN4I GGNNCC 2 cut(s) 352, 643
PstNI CAGNNNCTG 1 cut(s) 42
PsuI RGATCY 1 cut(s) 477
PvuII CAGCTG 1 cut(s) 42
SacI GAGCTC 1 cut(s) 417
SaqAI TTAA 3 cut(s) 147, 228, 255
SatI GCNGC 4 cut(s) 37, 40, 43, 267
Sau3AI GATC 4 cut(s) 118, 124, 477, 538
SchI GAGTC 1 cut(s) 485
ScrFI CCNGG 2 cut(s) 10, 278
SduI GDGCHC 1 cut(s) 417
SetI ASST 6 cut(s) 44, 153, 417, 433, 459, 689
SmlI CTYRAG 1 cut(s) 227
SmoI CTYRAG 1 cut(s) 227
Sse9I AATT 8 cut(s) 46, 144, 168, 200, 502, 623, 657, 667
SsiI CCGC 1 cut(s) 267
SstI GAGCTC 1 cut(s) 417
StyD4I CCNGG 2 cut(s) 8, 276
TaaI ACNGT 1 cut(s) 410
TaqI TCGA 2 cut(s) 166, 183
TasI AATT 8 cut(s) 46, 144, 168, 200, 502, 623, 657, 667
TauI GCSGC 1 cut(s) 269
Tru1I TTAA 3 cut(s) 147, 228, 255
Tru9I TTAA 3 cut(s) 147, 228, 255
TscAI CASTG 1 cut(s) 415
TseFI GTSAC 2 cut(s) 334, 401
TseI GCWGC 3 cut(s) 36, 39, 42
Tsp45I GTSAC 2 cut(s) 334, 401
TspDTI ATGAA 7 cut(s) 45, 170, 213, 402, 441, 564, 669
TspGWI ACGGA 1 cut(s) 531
TspRI CASTG 1 cut(s) 415
Van91I CCANNNNNTGG 1 cut(s) 360
Vha464I CTTAAG 1 cut(s) 227
XapI RAATTY 1 cut(s) 200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.