RLG00000025358

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0007516
Physical Location & Seq
Reverse (-)
45275587 .. 45276200
614 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025358

Sequence Viewer

Length: 429 bp
ATGTCTTCTACAACCGACGAGGAGGACGTACGGAACCTTCTCAGGCAAGTATTTCACCACAGAAGCCAGTCTCCGACAGAACTAAATGATCAAGGGCAAGGACAGGACTCAGATGACGATGACTTGCAGCAACTCTTGTCCTCCAAGATGCAATCCCCCAAGGGTGTCAATGCTGCCAAGCATTTCACCAAGATGTTTGCTGGTTTGATGGATGATGGCCACTTCAAAGCGGCTATAGAGATCAAGAAGCTGACCTTGGAGAAGGGTTTGATACCCGATGTGGTTATTCAGACCGGCCTGATGGAGGCCTATGCCAGTGCTGGAATGACAGAGGAGGTCCTCAGTGTATACCAGGGTATGACATCCTTTGGGATCACCCCCAATTCCTACACCAATGGTGTTCTCATCAGGGCATGCACTCGTCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_2 PPR_3

Protein Analysis

143

Amino Acids

15.64

Weight (kDa)

5.08

Isoelectric Point (pI)

55.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 92 - 140 1.9e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 348
AciI CCGC 1 cut(s) 230
AclWI GGATC 1 cut(s) 380
AcoI YGGCCR 1 cut(s) 217
AfaI GTAC 1 cut(s) 30
AfiI CCNNNNNNNGG 1 cut(s) 304
AgsI TTSAA 1 cut(s) 226
AjnI CCWGG 1 cut(s) 351
AjuI GAANNNNNNNTTGG 2 cut(s) 239, 271
AluBI AGCT 1 cut(s) 250
AluI AGCT 1 cut(s) 250
Alw26I GTCTC 1 cut(s) 75
AlwI GGATC 1 cut(s) 380
AoxI GGCC 3 cut(s) 217, 295, 306
ApeKI GCWGC 2 cut(s) 127, 173
AspS9I GGNCC 1 cut(s) 337
AsuHPI GGTGA 3 cut(s) 47, 178, 367
AvaII GGWCC 1 cut(s) 337
BalI TGGCCA 1 cut(s) 219
BarI GAAGNNNNNNTAC 2 cut(s) 21, 53
BbvI GCAGC 2 cut(s) 139, 160
BccI CCATC 3 cut(s) 202, 209, 295
BciT130I CCWGG 1 cut(s) 353
BclI TGATCA 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 75
BfmI CTRYAG 1 cut(s) 234
BisI GCNGC 3 cut(s) 128, 174, 231
BlsI GCNGC 3 cut(s) 129, 175, 232
Bme1390I CCNGG 1 cut(s) 353
Bme18I GGWCC 1 cut(s) 337
BmgT120I GGNCC 1 cut(s) 337
BmiI GGNNCC 1 cut(s) 35
BmrFI CCNGG 1 cut(s) 353
BmsI GCATC 1 cut(s) 138
BsaJI CCNNGG 3 cut(s) 159, 255, 352
Bsc4I CCNNNNNNNGG 1 cut(s) 304
Bse118I RCCGGY 1 cut(s) 293
Bse1I ACTGG 2 cut(s) 67, 315
BseBI CCWGG 1 cut(s) 353
BseDI CCNNGG 3 cut(s) 159, 255, 352
BseGI GGATG 2 cut(s) 217, 362
BseLI CCNNNNNNNGG 1 cut(s) 304
BseMII CTCAG 3 cut(s) 55, 123, 355
BseNI ACTGG 2 cut(s) 67, 315
BseRI GAGGAG 2 cut(s) 35, 347
BseXI GCAGC 2 cut(s) 139, 160
BshFI GGCC 3 cut(s) 219, 297, 308
BsiSI CCGG 1 cut(s) 294
BsiWI CGTACG 1 cut(s) 28
BslI CCNNNNNNNGG 1 cut(s) 304
BsmAI GTCTC 1 cut(s) 75
BsnI GGCC 3 cut(s) 219, 297, 308
Bsp143I GATC 3 cut(s) 88, 240, 372
BspACI CCGC 1 cut(s) 230
BspANI GGCC 3 cut(s) 219, 297, 308
BspCNI CTCAG 3 cut(s) 54, 122, 354
BspLI GGNNCC 1 cut(s) 35
BspPI GGATC 1 cut(s) 380
BsrFI RCCGGY 1 cut(s) 293
BsrI ACTGG 2 cut(s) 67, 315
BssAI RCCGGY 1 cut(s) 293
BssECI CCNNGG 3 cut(s) 159, 255, 352
BssMI GATC 3 cut(s) 88, 240, 372
BssNAI GTATAC 1 cut(s) 349
BssT1I CCWWGG 2 cut(s) 159, 255
Bst1107I GTATAC 1 cut(s) 349
Bst2UI CCWGG 1 cut(s) 353
BstC8I GCNNGC 1 cut(s) 415
BstDEI CTNAG 3 cut(s) 41, 109, 341
BstENI CCTNNNNNAGG 1 cut(s) 302
BstF5I GGATG 2 cut(s) 217, 362
BstKTI GATC 3 cut(s) 91, 243, 375
BstMAI GTCTC 1 cut(s) 75
BstMBI GATC 3 cut(s) 88, 240, 372
BstNI CCWGG 1 cut(s) 353
BstNSI RCATGY 1 cut(s) 417
BstSCI CCNGG 1 cut(s) 351
BstSFI CTRYAG 1 cut(s) 234
BstV1I GCAGC 2 cut(s) 139, 160
BstZ17I GTATAC 1 cut(s) 349
BsuRI GGCC 3 cut(s) 219, 297, 308
BtsCI GGATG 2 cut(s) 217, 362
BtsIMutI CAGTG 2 cut(s) 322, 349
Cac8I GCNNGC 1 cut(s) 415
Cfr10I RCCGGY 1 cut(s) 293
Cfr13I GGNCC 1 cut(s) 337
Csp6I GTAC 1 cut(s) 29
CviAII CATG 1 cut(s) 414
CviJI RGCY 6 cut(s) 66, 219, 233, 250, 297, 308
CviKI_1 RGCY 6 cut(s) 66, 219, 233, 250, 297, 308
CviQI GTAC 1 cut(s) 29
DdeI CTNAG 3 cut(s) 41, 109, 341
DpnI GATC 3 cut(s) 90, 242, 374
DpnII GATC 3 cut(s) 88, 240, 372
EaeI YGGCCR 1 cut(s) 217
Eco130I CCWWGG 2 cut(s) 159, 255
Eco147I AGGCCT 1 cut(s) 308
Eco47I GGWCC 1 cut(s) 337
EcoNI CCTNNNNNAGG 1 cut(s) 302
EcoO109I RGGNCCY 1 cut(s) 337
EcoRII CCWGG 1 cut(s) 351
EcoT14I CCWWGG 2 cut(s) 159, 255
ErhI CCWWGG 2 cut(s) 159, 255
FaeI CATG 1 cut(s) 417
FaiI YATR 5 cut(s) 236, 312, 349, 359, 415
FalI AAGNNNNNCTT 2 cut(s) 239, 271
FatI CATG 1 cut(s) 413
FbaI TGATCA 1 cut(s) 88
FblI GTMKAC 1 cut(s) 348
Fnu4HI GCNGC 3 cut(s) 128, 174, 231
FokI GGATG 2 cut(s) 224, 349
Fsp4HI GCNGC 3 cut(s) 128, 174, 231
GluI GCNGC 3 cut(s) 128, 174, 231
HaeIII GGCC 3 cut(s) 219, 297, 308
HapII CCGG 1 cut(s) 294
Hin1II CATG 1 cut(s) 417
HinfI GANTC 1 cut(s) 107
HpaII CCGG 1 cut(s) 294
HphI GGTGA 3 cut(s) 47, 178, 367
Hpy166II GTNNAC 1 cut(s) 349
Hpy188I TCNGA 4 cut(s) 75, 112, 291, 428
Hpy188III TCNNGA 1 cut(s) 244
Hpy8I GTNNAC 1 cut(s) 349
Hpy99I CGWCG 1 cut(s) 20
HpyAV CCTTC 2 cut(s) 47, 256
HpyCH4IV ACGT 1 cut(s) 27
HpyCH4V TGCA 3 cut(s) 127, 151, 417
HpyF3I CTNAG 3 cut(s) 41, 109, 341
HpySE526I ACGT 1 cut(s) 27
Hsp92II CATG 1 cut(s) 417
Ksp22I TGATCA 1 cut(s) 88
Kzo9I GATC 3 cut(s) 88, 240, 372
Lsp1109I GCAGC 2 cut(s) 139, 160
LweI GCATC 1 cut(s) 138
MaeII ACGT 1 cut(s) 27
MalI GATC 3 cut(s) 90, 242, 374
MboI GATC 3 cut(s) 88, 240, 372
MlsI TGGCCA 1 cut(s) 219
MluCI AATT 1 cut(s) 382
MluNI TGGCCA 1 cut(s) 219
MlyI GAGTC 1 cut(s) 101
MmeI TCCRAC 1 cut(s) 98
MnlI CCTC 7 cut(s) 13, 16, 151, 298, 325, 328, 350
Mox20I TGGCCA 1 cut(s) 219
MscI TGGCCA 1 cut(s) 219
MslI CAYNNNNRTG 1 cut(s) 191
Msp20I TGGCCA 1 cut(s) 219
MspI CCGG 1 cut(s) 294
MspR9I CCNGG 1 cut(s) 353
MvaI CCWGG 1 cut(s) 353
NdeII GATC 3 cut(s) 88, 240, 372
NlaIII CATG 1 cut(s) 417
NlaIV GGNNCC 1 cut(s) 35
NspI RCATGY 1 cut(s) 417
PaeI GCATGC 1 cut(s) 417
PceI AGGCCT 1 cut(s) 308
PcsI WCGNNNNNNNCGW 1 cut(s) 24
Pfl23II CGTACG 1 cut(s) 28
PkrI GCNGC 3 cut(s) 129, 175, 232
PleI GAGTC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 101
PpuMI RGGWCCY 1 cut(s) 337
Psp5II RGGWCCY 1 cut(s) 337
Psp6I CCWGG 1 cut(s) 351
PspGI CCWGG 1 cut(s) 351
PspLI CGTACG 1 cut(s) 28
PspN4I GGNNCC 1 cut(s) 35
PspPI GGNCC 1 cut(s) 337
PspPPI RGGWCCY 1 cut(s) 337
RsaI GTAC 1 cut(s) 30
RsaNI GTAC 1 cut(s) 29
RseI CAYNNNNRTG 1 cut(s) 191
SatI GCNGC 3 cut(s) 128, 174, 231
Sau3AI GATC 3 cut(s) 88, 240, 372
Sau96I GGNCC 1 cut(s) 337
SchI GAGTC 1 cut(s) 101
ScrFI CCNGG 1 cut(s) 353
SetI ASST 5 cut(s) 30, 39, 252, 257, 339
SfaNI GCATC 1 cut(s) 138
SfcI CTRYAG 1 cut(s) 234
SinI GGWCC 1 cut(s) 337
SmiMI CAYNNNNRTG 1 cut(s) 191
SphI GCATGC 1 cut(s) 417
Sse9I AATT 1 cut(s) 382
SseBI AGGCCT 1 cut(s) 308
SsiI CCGC 1 cut(s) 230
StuI AGGCCT 1 cut(s) 308
StyD4I CCNGG 1 cut(s) 351
StyI CCWWGG 2 cut(s) 159, 255
TaiI ACGT 1 cut(s) 30
TasI AATT 1 cut(s) 382
TauI GCSGC 1 cut(s) 233
TscAI CASTG 2 cut(s) 322, 349
TseI GCWGC 2 cut(s) 127, 173
TspGWI ACGGA 1 cut(s) 46
TspRI CASTG 2 cut(s) 322, 349
VpaK11BI GGWCC 1 cut(s) 337
XagI CCTNNNNNAGG 1 cut(s) 302
XceI RCATGY 1 cut(s) 417
XmiI GTMKAC 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.