Rroxscaffold_6G00424250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0007516
Physical Location & Seq
Reverse (-)
45417525 .. 45422987
5463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00424250.1

Sequence Viewer

Length: 408 bp
ATGATCCTTGGGATGTCTAGGATGAAACTGGAGAGTTTCAACTCATTCGGAGTTTTTTGTGGCTATGGAGATCAAGAAGCTGACCTTGGACAAGGGTTTGATACCCGATGTGGTCATTTAGACCGGCCCGATGGAGGCCTATGCCATTGCGGAATAACAAGAGGAGGTTCTCGGTGTATATCGGGGCATCACTTCCTCCGGGATCACCCCAACCCCTACACCAATGGTGTTCTCATCGGGGCACTTGTCTCTAATAACTTCCTCAATGATGCTATTCGGTATTGGCTGGAAATGGGGAAAAAGGGAATGGCGCCCAATCATTCGACCTACAAAACAACGACTAGGGCGCTGCAAGACGCGGGTAGGGAAATCGAGGAGTTCACACGGCAGCTTATAGCCCAACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

135

Amino Acids

14.98

Weight (kDa)

7.0

Isoelectric Point (pI)

29.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 76 - 113 9.1e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 310
AccII CGCG 1 cut(s) 359
AciI CCGC 2 cut(s) 150, 359
AclWI GGATC 1 cut(s) 210
AcyI GRCGYC 1 cut(s) 311
AfiI CCNNNNNNNGG 1 cut(s) 134
AgsI TTSAA 1 cut(s) 40
AjuI GAANNNNNNNTTGG 2 cut(s) 69, 101
AluBI AGCT 2 cut(s) 80, 391
AluI AGCT 2 cut(s) 80, 391
Alw26I GTCTC 1 cut(s) 253
AlwI GGATC 1 cut(s) 210
AoxI GGCC 2 cut(s) 125, 136
ApeKI GCWGC 2 cut(s) 349, 388
AspLEI GCGC 2 cut(s) 313, 349
AspS9I GGNCC 1 cut(s) 126
AsuC2I CCSGG 1 cut(s) 200
AsuHPI GGTGA 1 cut(s) 197
BaeGI GKGCMC 1 cut(s) 244
BanI GGYRCC 1 cut(s) 310
BbvI GCAGC 2 cut(s) 336, 400
BccI CCATC 1 cut(s) 125
BceAI ACGGC 1 cut(s) 401
BcnI CCSGG 1 cut(s) 200
BcoDI GTCTC 1 cut(s) 253
BfaI CTAG 2 cut(s) 18, 342
BfoI RGCGCY 2 cut(s) 314, 350
BisI GCNGC 2 cut(s) 350, 389
BlsI GCNGC 2 cut(s) 351, 390
Bme1390I CCNGG 1 cut(s) 200
BmgT120I GGNCC 1 cut(s) 126
BmiI GGNNCC 1 cut(s) 312
BmrFI CCNGG 1 cut(s) 200
BmsI GCATC 2 cut(s) 196, 259
BpmI CTGGAG 1 cut(s) 50
BpuMI CCSGG 1 cut(s) 200
BsaHI GRCGYC 1 cut(s) 311
BsaJI CCNNGG 2 cut(s) 7, 85
Bsc4I CCNNNNNNNGG 1 cut(s) 134
Bse118I RCCGGY 1 cut(s) 123
Bse1I ACTGG 1 cut(s) 33
Bse3DI GCAATG 1 cut(s) 145
BseDI CCNNGG 2 cut(s) 7, 85
BseGI GGATG 2 cut(s) 18, 27
BseLI CCNNNNNNNGG 1 cut(s) 134
BseMI GCAATG 1 cut(s) 145
BseNI ACTGG 1 cut(s) 33
BseRI GAGGAG 2 cut(s) 177, 389
BseSI GKGCMC 1 cut(s) 244
BseXI GCAGC 2 cut(s) 336, 400
Bsh1236I CGCG 1 cut(s) 359
BshFI GGCC 2 cut(s) 127, 138
BshNI GGYRCC 1 cut(s) 310
BsiSI CCGG 2 cut(s) 124, 199
BslI CCNNNNNNNGG 1 cut(s) 134
BsmAI GTCTC 1 cut(s) 253
BsnI GGCC 2 cut(s) 127, 138
Bsp1286I GDGCHC 1 cut(s) 244
Bsp143I GATC 3 cut(s) 3, 70, 202
BspACI CCGC 2 cut(s) 150, 359
BspANI GGCC 2 cut(s) 127, 138
BspFNI CGCG 1 cut(s) 359
BspLI GGNNCC 1 cut(s) 312
BspPI GGATC 1 cut(s) 210
BspT107I GGYRCC 1 cut(s) 310
BsrDI GCAATG 1 cut(s) 145
BsrFI RCCGGY 1 cut(s) 123
BsrI ACTGG 1 cut(s) 33
BssAI RCCGGY 1 cut(s) 123
BssECI CCNNGG 2 cut(s) 7, 85
BssMI GATC 3 cut(s) 3, 70, 202
BssNI GRCGYC 1 cut(s) 311
BssT1I CCWWGG 2 cut(s) 7, 85
BstACI GRCGYC 1 cut(s) 311
BstF5I GGATG 2 cut(s) 18, 27
BstFNI CGCG 1 cut(s) 359
BstH2I RGCGCY 2 cut(s) 314, 350
BstHHI GCGC 2 cut(s) 313, 349
BstKTI GATC 3 cut(s) 6, 73, 205
BstMAI GTCTC 1 cut(s) 253
BstMBI GATC 3 cut(s) 3, 70, 202
BstSCI CCNGG 1 cut(s) 198
BstSLI GKGCMC 1 cut(s) 244
BstUI CGCG 1 cut(s) 359
BstV1I GCAGC 2 cut(s) 336, 400
BsuRI GGCC 2 cut(s) 127, 138
BtsCI GGATG 2 cut(s) 18, 27
CfoI GCGC 2 cut(s) 313, 349
Cfr10I RCCGGY 1 cut(s) 123
Cfr13I GGNCC 1 cut(s) 126
CseI GACGC 1 cut(s) 365
CviJI RGCY 7 cut(s) 63, 80, 127, 138, 286, 391, 398
CviKI_1 RGCY 7 cut(s) 63, 80, 127, 138, 286, 391, 398
DinI GGCGCC 1 cut(s) 312
DpnI GATC 3 cut(s) 5, 72, 204
DpnII GATC 3 cut(s) 3, 70, 202
Eco130I CCWWGG 2 cut(s) 7, 85
Eco147I AGGCCT 1 cut(s) 138
EcoT14I CCWWGG 2 cut(s) 7, 85
EgeI GGCGCC 1 cut(s) 312
EheI GGCGCC 1 cut(s) 312
ErhI CCWWGG 2 cut(s) 7, 85
FaiI YATR 4 cut(s) 66, 142, 179, 395
FalI AAGNNNNNCTT 2 cut(s) 69, 101
FauI CCCGC 1 cut(s) 352
Fnu4HI GCNGC 2 cut(s) 350, 389
FokI GGATG 2 cut(s) 25, 34
Fsp4HI GCNGC 2 cut(s) 350, 389
FspBI CTAG 2 cut(s) 18, 342
GlaI GCGC 2 cut(s) 312, 348
GluI GCNGC 2 cut(s) 350, 389
GsuI CTGGAG 1 cut(s) 50
HaeII RGCGCY 2 cut(s) 314, 350
HaeIII GGCC 2 cut(s) 127, 138
HapII CCGG 2 cut(s) 124, 199
HgaI GACGC 1 cut(s) 365
HhaI GCGC 2 cut(s) 313, 349
Hin1I GRCGYC 1 cut(s) 311
Hin6I GCGC 2 cut(s) 311, 347
HinP1I GCGC 2 cut(s) 311, 347
HpaII CCGG 2 cut(s) 124, 199
HphI GGTGA 1 cut(s) 197
Hpy166II GTNNAC 1 cut(s) 381
Hpy188I TCNGA 1 cut(s) 50
Hpy188III TCNNGA 1 cut(s) 74
Hpy8I GTNNAC 1 cut(s) 381
HpyCH4V TGCA 1 cut(s) 352
Hsp92I GRCGYC 1 cut(s) 311
HspAI GCGC 2 cut(s) 311, 347
KasI GGCGCC 1 cut(s) 310
Kzo9I GATC 3 cut(s) 3, 70, 202
LpnPI CCDG 4 cut(s) 14, 137, 212, 272
Lsp1109I GCAGC 2 cut(s) 336, 400
LweI GCATC 2 cut(s) 196, 259
MaeI CTAG 2 cut(s) 18, 342
MalI GATC 3 cut(s) 5, 72, 204
MboI GATC 3 cut(s) 3, 70, 202
MhlI GDGCHC 1 cut(s) 244
Mly113I GGCGCC 1 cut(s) 311
MnlI CCTC 6 cut(s) 128, 155, 158, 206, 272, 367
MspI CCGG 2 cut(s) 124, 199
MspR9I CCNGG 1 cut(s) 200
MvnI CGCG 1 cut(s) 359
NarI GGCGCC 1 cut(s) 311
NciI CCSGG 1 cut(s) 200
NdeII GATC 3 cut(s) 3, 70, 202
NlaIV GGNNCC 1 cut(s) 312
PceI AGGCCT 1 cut(s) 138
PfoI TCCNGGA 1 cut(s) 198
PkrI GCNGC 2 cut(s) 351, 390
PluTI GGCGCC 1 cut(s) 314
PspN4I GGNNCC 1 cut(s) 312
PspPI GGNCC 1 cut(s) 126
SatI GCNGC 2 cut(s) 350, 389
Sau3AI GATC 3 cut(s) 3, 70, 202
Sau96I GGNCC 1 cut(s) 126
ScrFI CCNGG 1 cut(s) 200
SduI GDGCHC 1 cut(s) 244
SetI ASST 5 cut(s) 82, 87, 169, 329, 393
SfaNI GCATC 2 cut(s) 196, 259
SfoI GGCGCC 1 cut(s) 312
SseBI AGGCCT 1 cut(s) 138
SsiI CCGC 2 cut(s) 150, 359
SspDI GGCGCC 1 cut(s) 310
SspMI CTAG 2 cut(s) 18, 342
StuI AGGCCT 1 cut(s) 138
StyD4I CCNGG 1 cut(s) 198
StyI CCWWGG 2 cut(s) 7, 85
TaqI TCGA 2 cut(s) 323, 372
TseI GCWGC 2 cut(s) 349, 388
TspDTI ATGAA 1 cut(s) 38
XspI CTAG 2 cut(s) 18, 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.