Rroxscaffold_5G00343890

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0007516
Physical Location & Seq
Reverse (-)
13702218 .. 13710473
8256 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00343890.1

Sequence Viewer

Length: 537 bp
ATGGGCCTTCGACTTCTTCATATCAAATTATCCACCATGAGTGTAGATCATTTTGCTAAAATTTCAGAGCTAATTTCCATGTGGTTGGGCCGGAAACACTGCTGGACTCCTTGCAGGTCCAGTTTTCTAGTTTTGTCTTTCAAAAACTGGACTGATGGTGTGAAGATATCGGATTGGGGTGAGAACAATATTGCCATTGCTGCTGAAACTACTGCTCCTTGGGCGTTGTTTCAGCAAGAAGAAGAAGAAAAGCATTTAACAATTTTGAAAGTTCAAAACAGATACGAGCAAGGGCTGCAAGGCGGTGTCGATGACGGTAATGGGAATACAATCGGAGGAGGTGATCAACGAGCACGAGCCTATCGATCTCTATGCCAAGTCGGCAATAACAAAATCTCGCAAATCGAGTACAAAGCACAACTCAACCATTCGGGATTTAGATGCCTTCCGGACATTGAGACTACAAAATATTGGAGTACGGCTCGAGGAGAGCGACTCGGCTTCGATTTTGAGCTCCCGGCCTCTCGGGCTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

178

Amino Acids

20.15

Weight (kDa)

7.71

Isoelectric Point (pI)

36.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 105
AccIII TCCGGA 1 cut(s) 448
AciI CCGC 1 cut(s) 303
AcsI RAATTY 1 cut(s) 60
AfaI GTAC 2 cut(s) 410, 478
AgsI TTSAA 3 cut(s) 142, 268, 275
AluBI AGCT 2 cut(s) 70, 514
AluI AGCT 2 cut(s) 70, 514
Alw21I GWGCWC 2 cut(s) 355, 516
Alw26I GTCTC 1 cut(s) 452
Ama87I CYCGRG 2 cut(s) 483, 525
Aor13HI TCCGGA 1 cut(s) 448
AoxI GGCC 3 cut(s) 4, 88, 519
ApeKI GCWGC 2 cut(s) 200, 295
ApoI RAATTY 1 cut(s) 60
AspS9I GGNCC 3 cut(s) 4, 88, 117
AsuC2I CCSGG 1 cut(s) 518
AsuHPI GGTGA 2 cut(s) 191, 353
AvaI CYCGRG 2 cut(s) 483, 525
AvaII GGWCC 1 cut(s) 117
BanII GRGCYC 2 cut(s) 516, 532
BauI CACGAG 1 cut(s) 354
Bbv12I GWGCWC 2 cut(s) 355, 516
BbvI GCAGC 2 cut(s) 187, 282
BccI CCATC 1 cut(s) 149
BceAI ACGGC 1 cut(s) 495
BcgI CGANNNNNNTGC 2 cut(s) 354, 388
BclI TGATCA 1 cut(s) 343
BcnI CCSGG 1 cut(s) 518
BcoDI GTCTC 1 cut(s) 452
BfaI CTAG 1 cut(s) 128
BfuAI ACCTGC 1 cut(s) 105
BglI GCCNNNNNGGC 2 cut(s) 381, 527
BisI GCNGC 2 cut(s) 201, 296
BlsI GCNGC 2 cut(s) 202, 297
Bme1390I CCNGG 1 cut(s) 518
Bme18I GGWCC 1 cut(s) 117
BmeT110I CYCGRG 2 cut(s) 483, 525
BmgT120I GGNCC 3 cut(s) 4, 88, 117
BmrFI CCNGG 1 cut(s) 518
BmsI GCATC 1 cut(s) 431
BplI GAGNNNNNCTC 4 cut(s) 466, 498, 480, 512
BpuMI CCSGG 1 cut(s) 518
Bsa29I ATCGAT 1 cut(s) 364
BsaJI CCNNGG 1 cut(s) 218
BsaWI WCCGGW 1 cut(s) 448
BsaXI ACNNNNNCTCC 2 cut(s) 199, 229
Bse1I ACTGG 2 cut(s) 120, 152
Bse3DI GCAATG 1 cut(s) 195
BseAI TCCGGA 1 cut(s) 448
BseCI ATCGAT 1 cut(s) 364
BseDI CCNNGG 1 cut(s) 218
BseMI GCAATG 1 cut(s) 195
BseNI ACTGG 2 cut(s) 120, 152
BseRI GAGGAG 2 cut(s) 351, 501
BseXI GCAGC 2 cut(s) 187, 282
BshFI GGCC 3 cut(s) 6, 90, 521
BshVI ATCGAT 1 cut(s) 364
BsiHKAI GWGCWC 2 cut(s) 355, 516
BsiHKCI CYCGRG 2 cut(s) 483, 525
BsiSI CCGG 3 cut(s) 91, 449, 518
BsmAI GTCTC 1 cut(s) 452
BsnI GGCC 3 cut(s) 6, 90, 521
BsoBI CYCGRG 2 cut(s) 483, 525
Bsp1286I GDGCHC 3 cut(s) 355, 516, 532
Bsp13I TCCGGA 1 cut(s) 448
Bsp143I GATC 3 cut(s) 46, 343, 365
BspACI CCGC 1 cut(s) 303
BspANI GGCC 3 cut(s) 6, 90, 521
BspDI ATCGAT 1 cut(s) 364
BspEI TCCGGA 1 cut(s) 448
BspMI ACCTGC 1 cut(s) 105
BsrDI GCAATG 1 cut(s) 195
BsrI ACTGG 2 cut(s) 120, 152
BssECI CCNNGG 1 cut(s) 218
BssMI GATC 3 cut(s) 46, 343, 365
BssSI CACGAG 1 cut(s) 354
BssT1I CCWWGG 1 cut(s) 218
Bst2BI CACGAG 1 cut(s) 354
Bst4CI ACNGT 1 cut(s) 317
BstAPI GCANNNNNTGC 1 cut(s) 295
BstKTI GATC 3 cut(s) 49, 346, 368
BstMAI GTCTC 1 cut(s) 452
BstMBI GATC 3 cut(s) 46, 343, 365
BstMWI GCNNNNNNNGC 5 cut(s) 200, 221, 295, 381, 527
BstSCI CCNGG 1 cut(s) 516
BstV1I GCAGC 2 cut(s) 187, 282
BstXI CCANNNNNNTGG 1 cut(s) 85
Bsu15I ATCGAT 1 cut(s) 364
BsuRI GGCC 3 cut(s) 6, 90, 521
BsuTUI ATCGAT 1 cut(s) 364
BtsI GCAGTG 1 cut(s) 97
BtsIMutI CAGTG 1 cut(s) 97
BveI ACCTGC 1 cut(s) 105
Cfr13I GGNCC 3 cut(s) 4, 88, 117
ClaI ATCGAT 1 cut(s) 364
Csp6I GTAC 2 cut(s) 409, 477
CviAII CATG 2 cut(s) 37, 79
CviQI GTAC 2 cut(s) 409, 477
DpnI GATC 3 cut(s) 48, 345, 367
DpnII GATC 3 cut(s) 46, 343, 365
Ecl136II GAGCTC 1 cut(s) 514
Eco130I CCWWGG 1 cut(s) 218
Eco24I GRGCYC 2 cut(s) 516, 532
Eco32I GATATC 1 cut(s) 168
Eco47I GGWCC 1 cut(s) 117
Eco53kI GAGCTC 1 cut(s) 514
Eco88I CYCGRG 2 cut(s) 483, 525
EcoICRI GAGCTC 1 cut(s) 514
EcoRV GATATC 1 cut(s) 168
EcoT14I CCWWGG 1 cut(s) 218
EcoT38I GRGCYC 2 cut(s) 516, 532
ErhI CCWWGG 1 cut(s) 218
FaeI CATG 2 cut(s) 40, 82
FaiI YATR 4 cut(s) 21, 38, 80, 373
FatI CATG 2 cut(s) 36, 78
FbaI TGATCA 1 cut(s) 343
Fnu4HI GCNGC 2 cut(s) 201, 296
FriOI GRGCYC 2 cut(s) 516, 532
Fsp4HI GCNGC 2 cut(s) 201, 296
FspBI CTAG 1 cut(s) 128
GluI GCNGC 2 cut(s) 201, 296
HaeIII GGCC 3 cut(s) 6, 90, 521
HapII CCGG 3 cut(s) 91, 449, 518
Hin1II CATG 2 cut(s) 40, 82
HinfI GANTC 2 cut(s) 106, 495
HpaII CCGG 3 cut(s) 91, 449, 518
HphI GGTGA 2 cut(s) 191, 353
Hpy188I TCNGA 3 cut(s) 67, 172, 335
Hpy188III TCNNGA 2 cut(s) 432, 449
HpyAV CCTTC 2 cut(s) 17, 455
HpyCH4III ACNGT 1 cut(s) 317
HpyCH4V TGCA 2 cut(s) 114, 298
HpyF10VI GCNNNNNNNGC 5 cut(s) 200, 221, 295, 381, 527
Hsp92II CATG 2 cut(s) 40, 82
Kpn2I TCCGGA 1 cut(s) 448
Ksp22I TGATCA 1 cut(s) 343
Kzo9I GATC 3 cut(s) 46, 343, 365
LmnI GCTCC 2 cut(s) 220, 519
LpnPI CCDG 7 cut(s) 88, 100, 104, 133, 133, 462, 531
Lsp1109I GCAGC 2 cut(s) 187, 282
LweI GCATC 1 cut(s) 431
MaeI CTAG 1 cut(s) 128
MalI GATC 3 cut(s) 48, 345, 367
MboI GATC 3 cut(s) 46, 343, 365
MboII GAAGA 5 cut(s) 8, 175, 251, 254, 257
MhlI GDGCHC 3 cut(s) 355, 516, 532
MluCI AATT 4 cut(s) 26, 60, 72, 261
MlyI GAGTC 2 cut(s) 100, 489
MnlI CCTC 4 cut(s) 329, 332, 479, 532
MroI TCCGGA 1 cut(s) 448
MseI TTAA 1 cut(s) 257
MspI CCGG 3 cut(s) 91, 449, 518
MspR9I CCNGG 1 cut(s) 518
MwoI GCNNNNNNNGC 5 cut(s) 200, 221, 295, 381, 527
NciI CCSGG 1 cut(s) 518
NdeII GATC 3 cut(s) 46, 343, 365
NlaIII CATG 2 cut(s) 40, 82
NmeAIII GCCGAG 1 cut(s) 477
PaeR7I CTCGAG 1 cut(s) 483
PcsI WCGNNNNNNNCGW 2 cut(s) 361, 490
PkrI GCNGC 2 cut(s) 202, 297
PleI GAGTC 2 cut(s) 100, 489
PpsI GAGTC 2 cut(s) 100, 489
Psp124BI GAGCTC 1 cut(s) 516
PspPI GGNCC 3 cut(s) 4, 88, 117
PspXI VCTCGAGB 1 cut(s) 483
RsaI GTAC 2 cut(s) 410, 478
RsaNI GTAC 2 cut(s) 409, 477
SacI GAGCTC 1 cut(s) 516
SaqAI TTAA 1 cut(s) 257
SatI GCNGC 2 cut(s) 201, 296
Sau3AI GATC 3 cut(s) 46, 343, 365
Sau96I GGNCC 3 cut(s) 4, 88, 117
SchI GAGTC 2 cut(s) 100, 489
ScrFI CCNGG 1 cut(s) 518
SduI GDGCHC 3 cut(s) 355, 516, 532
SetI ASST 4 cut(s) 72, 119, 343, 516
SfaNI GCATC 1 cut(s) 431
Sfr274I CTCGAG 1 cut(s) 483
SinI GGWCC 1 cut(s) 117
SlaI CTCGAG 1 cut(s) 483
SmlI CTYRAG 1 cut(s) 483
SmoI CTYRAG 1 cut(s) 483
Sse9I AATT 4 cut(s) 26, 60, 72, 261
SsiI CCGC 1 cut(s) 303
SspI AATATT 2 cut(s) 190, 470
SspMI CTAG 1 cut(s) 128
SstI GAGCTC 1 cut(s) 516
StyD4I CCNGG 1 cut(s) 516
StyI CCWWGG 1 cut(s) 218
TaaI ACNGT 1 cut(s) 317
TaqI TCGA 6 cut(s) 10, 309, 364, 405, 484, 504
TasI AATT 4 cut(s) 26, 60, 72, 261
TatI WGTACW 1 cut(s) 408
Tru1I TTAA 1 cut(s) 257
Tru9I TTAA 1 cut(s) 257
TscAI CASTG 1 cut(s) 104
TseI GCWGC 2 cut(s) 200, 295
TspDTI ATGAA 1 cut(s) 8
TspRI CASTG 1 cut(s) 104
VpaK11BI GGWCC 1 cut(s) 117
XapI RAATTY 1 cut(s) 60
XhoI CTCGAG 1 cut(s) 483
XspI CTAG 1 cut(s) 128
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.