Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0011617

Physical Location & Seq

Genomic Coordinates Forward (+)

60639785 .. 60650423

Gene Structure 10639 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000035091

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1449 bp

ATGGTGGATGGATTGGTCTTCGGACTTTCCGAGGAGCGTCCGCTATGGCTGGGTTCGTCTCTAGGGCGAAACTTAATAGTAGATCGATCGGTGATTGTTGGGGTTTGGCCGGAAGATTTTGTTGGTTTTGGACAGGGGCTGGCCAGGGACGGTGGAGGTCGGGGATTAAGTTCTCTTCGGGTCGCTGAGATGGGTAAGGCTGGTTGTCGAGATCTGATCCGCTTTGCTTCTGATAGCGGGTGGGACGGCGTTGCTTGGGCACATGGCAGTGGAGGATTCTCGGCTGTGGTGTGTGCTCCCGGTGGCGGAGGCTGGGCAGCGGCGGCAAAGGCTCACTGTGCTGGTGGCCGAAAACTGGTTGGAGATGGTGGTGGGGTGCTCATAGCAAATTCCATGCATTGGGCTCTTTGGGCCTTAATTTGGATTAAGAGCTGTTGGACTAGATTTTGGGTTCCAAAGTTGTTGGACTTGGGATGGACTTGTGCTCATGGTTGGGGTTGTTGGGCCCGTGTTTGGAAGTTGGGTTCCTTGTGGGCTGGATATTGGACTGCAAGGGTCTCTTATTGGGCTGAGGCAGATTGGAACTTCTCAATTGGCGCAGCTCTGATGAATGAAGACAGAGTGGATGAAGTTGTTGTAGCCGCAAGACTTGCTGGCGAGAGTGATGACGTAAGTGAACGTGATGGCGAAGGCGAGGACGCCAAAGGTGAGGACGCCAAAGCTCGGCGACTGGAAGAGAGCGGCGACGATTCGGTGCTAGTGGCTTCAGAGAATGACTGGGAGGTCACTGCAGGGTTTGTAGGATTCGATTACGACTCCATTGTTGGACAGTGCTGCGAGTCGCCGGTCGGGGACGAAGTAATCTTTATAGGGTCATCCTCTCACTCGGTTCCTTCCGCACCTGCAATCAATGATGCATGTCTCTTCTCAGCTCCTCATCCAAGAGTGCTACAGGAGATTTCAATTCTGAATGGAATTATCATGGGAGGTGGGGCTGGTGCTTCCGTCCATGGTAGACTCCGTGTTGCAACTGAGAATTTGGTTTTTGCAATGTCAGAAACAGCTCTGGGACTTTTTTTAGATGTATTGCCTCTTATTATTTCTCGAAGCTTTTTGGATCCTTTGGATGTTGAAATTGACGGTGGGAGTGGATCTGCTTCTTTGACAGATCTATTGAAACATGACAAGGTTCTGAGCAAGAAGCCCCCTGCTCCTCACCGAGTTGTGCCTGATTATCTGTTGAATGCAACAACTAAAGAAGCCAGCAAGAGAGTTAAGCTTGCCAACCTTGGTCGCCGTGGATTCAAGAAAAAATTCAAGGAAGATAAGGACCCCCTTAAGTCTTTCAGTGCTCAGGCCTACACCCTAGTGAGGAAGGGTGATCTCTTGATTGTGAGAGGAGGAATGAGGAGTGTCGAGTTCAAGGTTATTGAGACTGATCCTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0006139 GO:0006364 GO:0006396 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010467 GO:0010501 GO:0016070 GO:0016072 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0022613 GO:0031974 GO:0031981 GO:0034470 GO:0034641 GO:0034660 GO:0042254 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0071840 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DEAD Helicase_C

Protein Analysis

483

Amino Acids

51.63

Weight (kDa)

5.51

Isoelectric Point (pI)

33.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_2	PF16113	320 - 362	5.8e-15	Enoyl-CoA hydratase/isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011617)

Species	Orthologous Gene IDs
rosa_chinensis	RchiOBHm_Chr4g0405541
rosa_laevigata	RLG00000012603 RLG00000014250 RLG00000018184 RLG00000022599 RLG00000030134 RLG00000035091 RLG00000035371
rosa_roxburghii	Rroxscaffold_2G00103110 Rroxscaffold_3G00244540
rosa_rugosa	Rorug04G0102200 Rorug06G0229300
rosa_samantha	Rh1BG178600 Rh2BG296500 Rh2CG274200 Rh2CG294400 Rh4DG256300 Rh5BG223800 Rh5BG277400 Rh6AG035400

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	910
AasI	GACNNNNNNGTC	1 cut(s)	782
Acc36I	ACCTGC	1 cut(s)	910
AccB7I	CCANNNNNTGG	1 cut(s)	399
AccBSI	CCGCTC	1 cut(s)	741
AccI	GTMKAC	1 cut(s)	1015
AciI	CCGC	9 cut(s)	41, 220, 237, 306, 320, 323, 642, 741, 897
AclWI	GGATC	5 cut(s)	211, 1112, 1125, 1159, 1433
AcoI	YGGCCR	3 cut(s)	107, 141, 346
AcsI	RAATTY	3 cut(s)	388, 1036, 1313
AcuI	CTGAAG	1 cut(s)	750
AcyI	GRCGYC	2 cut(s)	699, 714
AfiI	CCNNNNNNNGG	7 cut(s)	305, 355, 399, 420, 513, 723, 1371
AflII	CTTAAG	1 cut(s)	1337
AgsI	TTSAA	7 cut(s)	963, 1133, 1177, 1243, 1306, 1318, 1423
AjnI	CCWGG	1 cut(s)	143
AjuI	GAANNNNNNNTTGG	2 cut(s)	105, 137
AleI	CACNNNNGTG	1 cut(s)	1367
AloI	GAACNNNNNNTCC	2 cut(s)	508, 540
AluBI	AGCT	7 cut(s)	432, 602, 722, 932, 1064, 1110, 1279
AluI	AGCT	7 cut(s)	432, 602, 722, 932, 1064, 1110, 1279
Alw21I	GWGCWC	4 cut(s)	298, 381, 487, 1354
Alw26I	GTCTC	4 cut(s)	63, 562, 926, 1427
AlwI	GGATC	5 cut(s)	211, 1112, 1125, 1159, 1433
AlwNI	CAGNNNCTG	1 cut(s)	139
AoxI	GGCC	6 cut(s)	107, 141, 346, 411, 504, 1356
ApaI	GGGCCC	1 cut(s)	508
ApeKI	GCWGC	3 cut(s)	317, 599, 834
ApoI	RAATTY	3 cut(s)	388, 1036, 1313
Asp700I	GAANNNNTTC	1 cut(s)	1313
AspLEI	GCGC	1 cut(s)	599
AspS9I	GGNCC	4 cut(s)	411, 504, 505, 1330
AsuC2I	CCSGG	1 cut(s)	300
AsuHPI	GGTGA	4 cut(s)	103, 719, 1208, 1391
AvaII	GGWCC	1 cut(s)	1330
BaeGI	GKGCMC	2 cut(s)	262, 508
BalI	TGGCCA	1 cut(s)	143
BamHI	GGATCC	1 cut(s)	1117
BanII	GRGCYC	2 cut(s)	406, 508
BbsI	GAAGAC	2 cut(s)	10, 621
Bbv12I	GWGCWC	4 cut(s)	298, 381, 487, 1354
BbvCI	CCTCAGC	1 cut(s)	570
BbvI	GCAGC	3 cut(s)	329, 611, 821
BccI	CCATC	5 cut(s)	2, 184, 359, 468, 677
BceAI	ACGGC	2 cut(s)	262, 1281
BciT130I	CCWGG	1 cut(s)	145
BcnI	CCSGG	1 cut(s)	300
BcoDI	GTCTC	4 cut(s)	63, 562, 926, 1427
BfaI	CTAG	4 cut(s)	62, 441, 758, 1367
BfmI	CTRYAG	2 cut(s)	789, 950
BfrI	CTTAAG	1 cut(s)	1337
BfuAI	ACCTGC	1 cut(s)	910
BglII	AGATCT	2 cut(s)	211, 1168
BisI	GCNGC	7 cut(s)	318, 321, 324, 600, 642, 742, 835
BlsI	GCNGC	7 cut(s)	319, 322, 325, 601, 643, 743, 836
Bme1390I	CCNGG	2 cut(s)	145, 300
Bme18I	GGWCC	1 cut(s)	1330
BmgT120I	GGNCC	4 cut(s)	411, 504, 505, 1330
BmiI	GGNNCC	6 cut(s)	453, 506, 526, 891, 1119, 1332
BmrFI	CCNGG	2 cut(s)	145, 300
BmrI	ACTGGG	1 cut(s)	787
BmsI	GCATC	1 cut(s)	904
BmuI	ACTGGG	1 cut(s)	787
BpiI	GAAGAC	2 cut(s)	10, 621
Bpu10I	CCTNAGC	2 cut(s)	570, 1353
BpuMI	CCSGG	1 cut(s)	300
Bsa29I	ATCGAT	1 cut(s)	85
BsaHI	GRCGYC	2 cut(s)	699, 714
BsaI	GGTCTC	1 cut(s)	562
BsaJI	CCNNGG	5 cut(s)	30, 144, 1009, 1288, 1297
Bsc4I	CCNNNNNNNGG	7 cut(s)	305, 355, 399, 420, 513, 723, 1371
Bse118I	RCCGGY	1 cut(s)	844
Bse1I	ACTGG	3 cut(s)	360, 735, 782
Bse3DI	GCAATG	1 cut(s)	1056
BseBI	CCWGG	1 cut(s)	145
BseCI	ATCGAT	1 cut(s)	85
BseDI	CCNNGG	5 cut(s)	30, 144, 1009, 1288, 1297
BseGI	GGATG	6 cut(s)	13, 479, 631, 875, 937, 1132
BseLI	CCNNNNNNNGG	7 cut(s)	305, 355, 399, 420, 513, 723, 1371
BseMI	GCAATG	1 cut(s)	1056
BseMII	CTCAG	6 cut(s)	177, 561, 942, 1023, 1184, 1367
BseNI	ACTGG	3 cut(s)	360, 735, 782
BseRI	GAGGAG	5 cut(s)	47, 924, 1203, 1413, 1423
BseSI	GKGCMC	2 cut(s)	262, 508
BseXI	GCAGC	3 cut(s)	329, 611, 821
BseYI	CCCAGC	2 cut(s)	49, 312
Bsh1285I	CGRYCG	2 cut(s)	89, 849
BshFI	GGCC	6 cut(s)	109, 143, 348, 413, 506, 1358
BshVI	ATCGAT	1 cut(s)	85
BsiEI	CGRYCG	2 cut(s)	89, 849
BsiHKAI	GWGCWC	4 cut(s)	298, 381, 487, 1354
BsiSI	CCGG	3 cut(s)	110, 300, 845
BslFI	GGGAC	4 cut(s)	161, 257, 866, 1083
BslI	CCNNNNNNNGG	7 cut(s)	305, 355, 399, 420, 513, 723, 1371
BsmAI	GTCTC	4 cut(s)	63, 562, 926, 1427
BsmBI	CGTCTC	1 cut(s)	63
BsmFI	GGGAC	4 cut(s)	161, 257, 866, 1083
BsmI	GAATGC	1 cut(s)	1249
BsnI	GGCC	6 cut(s)	109, 143, 348, 413, 506, 1358
Bso31I	GGTCTC	1 cut(s)	562
Bsp120I	GGGCCC	1 cut(s)	504
Bsp1286I	GDGCHC	7 cut(s)	262, 298, 381, 406, 487, 508, 1354
Bsp143I	GATC	9 cut(s)	82, 86, 211, 216, 1117, 1151, 1168, 1381, 1438
Bsp19I	CCATGG	1 cut(s)	1009
BspACI	CCGC	9 cut(s)	41, 220, 237, 306, 320, 323, 642, 741, 897
BspANI	GGCC	6 cut(s)	109, 143, 348, 413, 506, 1358
BspCNI	CTCAG	6 cut(s)	178, 562, 941, 1024, 1185, 1366
BspDI	ATCGAT	1 cut(s)	85
BspLI	GGNNCC	6 cut(s)	453, 506, 526, 891, 1119, 1332
BspMAI	CTGCAG	1 cut(s)	793
BspMI	ACCTGC	1 cut(s)	910
BspPI	GGATC	5 cut(s)	211, 1112, 1125, 1159, 1433
BspTI	CTTAAG	1 cut(s)	1337
BspTNI	GGTCTC	1 cut(s)	562
BsrBI	CCGCTC	1 cut(s)	741
BsrDI	GCAATG	1 cut(s)	1056
BsrFI	RCCGGY	1 cut(s)	844
BsrI	ACTGG	3 cut(s)	360, 735, 782
BssAI	RCCGGY	1 cut(s)	844
BssECI	CCNNGG	5 cut(s)	30, 144, 1009, 1288, 1297
BssMI	GATC	9 cut(s)	82, 86, 211, 216, 1117, 1151, 1168, 1381, 1438
BssNI	GRCGYC	2 cut(s)	699, 714
BssT1I	CCWWGG	2 cut(s)	1009, 1288
Bst2UI	CCWGG	1 cut(s)	145
Bst4CI	ACNGT	4 cut(s)	152, 338, 831, 1142
Bst6I	CTCTTC	3 cut(s)	180, 729, 929
BstACI	GRCGYC	2 cut(s)	699, 714
BstAFI	CTTAAG	1 cut(s)	1337
BstAPI	GCANNNNNTGC	1 cut(s)	650
BstC8I	GCNNGC	4 cut(s)	141, 655, 1264, 1281
BstDEI	CTNAG	6 cut(s)	186, 570, 928, 1032, 1193, 1353
BstDSI	CCRYGG	2 cut(s)	1009, 1297
BstF5I	GGATG	6 cut(s)	13, 479, 631, 875, 937, 1132
BstHHI	GCGC	1 cut(s)	599
BstKTI	GATC	9 cut(s)	85, 89, 214, 219, 1120, 1154, 1171, 1384, 1441
BstMAI	GTCTC	4 cut(s)	63, 562, 926, 1427
BstMBI	GATC	9 cut(s)	82, 86, 211, 216, 1117, 1151, 1168, 1381, 1438
BstMCI	CGRYCG	2 cut(s)	89, 849
BstMWI	GCNNNNNNNGC	5 cut(s)	323, 329, 338, 410, 650
BstNI	CCWGG	1 cut(s)	145
BstNSI	RCATGY	1 cut(s)	921
BstSCI	CCNGG	2 cut(s)	143, 298
BstSFI	CTRYAG	2 cut(s)	789, 950
BstSLI	GKGCMC	2 cut(s)	262, 508
BstV1I	GCAGC	3 cut(s)	329, 611, 821
BstV2I	GAAGAC	2 cut(s)	10, 621
BstX2I	RGATCY	4 cut(s)	211, 1117, 1151, 1168
BstYI	RGATCY	4 cut(s)	211, 1117, 1151, 1168
Bsu15I	ATCGAT	1 cut(s)	85
BsuRI	GGCC	6 cut(s)	109, 143, 348, 413, 506, 1358
BsuTUI	ATCGAT	1 cut(s)	85
BtgI	CCRYGG	2 cut(s)	1009, 1297
BtsCI	GGATG	6 cut(s)	13, 479, 631, 875, 937, 1132
BtsI	GCAGTG	2 cut(s)	274, 786
BtsIMutI	CAGTG	5 cut(s)	274, 334, 786, 836, 1354
BveI	ACCTGC	1 cut(s)	910
Cac8I	GCNNGC	4 cut(s)	141, 655, 1264, 1281
CaiI	CAGNNNCTG	1 cut(s)	139
CfoI	GCGC	1 cut(s)	599
Cfr10I	RCCGGY	1 cut(s)	844
Cfr13I	GGNCC	4 cut(s)	411, 504, 505, 1330
ClaI	ATCGAT	1 cut(s)	85
CseI	GACGC	3 cut(s)	26, 707, 722
CviAII	CATG	7 cut(s)	263, 394, 488, 918, 982, 1010, 1181
DdeI	CTNAG	6 cut(s)	186, 570, 928, 1032, 1193, 1353
DpnI	GATC	9 cut(s)	84, 88, 213, 218, 1119, 1153, 1170, 1383, 1440
DpnII	GATC	9 cut(s)	82, 86, 211, 216, 1117, 1151, 1168, 1381, 1438
DrdI	GACNNNNNNGTC	1 cut(s)	782
DseDI	GACNNNNNNGTC	1 cut(s)	782
EaeI	YGGCCR	3 cut(s)	107, 141, 346
Eam1104I	CTCTTC	3 cut(s)	180, 729, 929
EarI	CTCTTC	3 cut(s)	180, 729, 929
EciI	GGCGGA	1 cut(s)	321
Eco130I	CCWWGG	2 cut(s)	1009, 1288
Eco147I	AGGCCT	1 cut(s)	1358
Eco24I	GRGCYC	2 cut(s)	406, 508
Eco31I	GGTCTC	1 cut(s)	562
Eco47I	GGWCC	1 cut(s)	1330
Eco57I	CTGAAG	1 cut(s)	750
EcoO109I	RGGNCCY	1 cut(s)	1330
EcoRII	CCWGG	1 cut(s)	143
EcoT14I	CCWWGG	2 cut(s)	1009, 1288
EcoT22I	ATGCAT	2 cut(s)	399, 919
EcoT38I	GRGCYC	2 cut(s)	406, 508
ErhI	CCWWGG	2 cut(s)	1009, 1288
Esp3I	CGTCTC	1 cut(s)	63
FaeI	CATG	7 cut(s)	266, 397, 491, 921, 985, 1013, 1184
FalI	AAGNNNNNCTT	2 cut(s)	544, 576
FaqI	GGGAC	4 cut(s)	161, 257, 866, 1083
FatI	CATG	7 cut(s)	262, 393, 487, 917, 981, 1009, 1180
FauI	CCCGC	1 cut(s)	230
FblI	GTMKAC	1 cut(s)	1015
Fnu4HI	GCNGC	7 cut(s)	318, 321, 324, 600, 642, 742, 835
FokI	GGATG	6 cut(s)	20, 486, 638, 862, 924, 1139
FriOI	GRGCYC	2 cut(s)	406, 508
Fsp4HI	GCNGC	7 cut(s)	318, 321, 324, 600, 642, 742, 835
FspBI	CTAG	4 cut(s)	62, 441, 758, 1367
GlaI	GCGC	1 cut(s)	598
GluI	GCNGC	7 cut(s)	318, 321, 324, 600, 642, 742, 835
GsaI	CCCAGC	2 cut(s)	53, 316
HaeIII	GGCC	6 cut(s)	109, 143, 348, 413, 506, 1358
HapII	CCGG	3 cut(s)	110, 300, 845
HgaI	GACGC	3 cut(s)	26, 707, 722
HhaI	GCGC	1 cut(s)	599
Hin1I	GRCGYC	2 cut(s)	699, 714
Hin1II	CATG	7 cut(s)	266, 397, 491, 921, 985, 1013, 1184
Hin6I	GCGC	1 cut(s)	597
HinP1I	GCGC	1 cut(s)	597
HindIII	AAGCTT	2 cut(s)	1108, 1277
HinfI	GANTC	7 cut(s)	276, 749, 804, 815, 839, 1017, 1302
HpaII	CCGG	3 cut(s)	110, 300, 845
HphI	GGTGA	4 cut(s)	103, 719, 1208, 1391
Hpy166II	GTNNAC	2 cut(s)	677, 1016
Hpy188I	TCNGA	9 cut(s)	23, 31, 216, 232, 606, 769, 969, 1057, 1194
Hpy188III	TCNNGA	5 cut(s)	209, 1104, 1306, 1387, 1446
Hpy8I	GTNNAC	2 cut(s)	677, 1016
Hpy99I	CGWCG	1 cut(s)	749
HpyAV	CCTTC	3 cut(s)	683, 903, 1369
HpyCH4III	ACNGT	4 cut(s)	152, 338, 831, 1142
HpyCH4IV	ACGT	2 cut(s)	669, 679
HpyCH4V	TGCA	8 cut(s)	397, 551, 791, 905, 917, 1028, 1049, 1247
HpyF10VI	GCNNNNNNNGC	5 cut(s)	323, 329, 338, 410, 650
HpyF3I	CTNAG	6 cut(s)	186, 570, 928, 1032, 1193, 1353
HpySE526I	ACGT	2 cut(s)	669, 679
Hsp92I	GRCGYC	2 cut(s)	699, 714
Hsp92II	CATG	7 cut(s)	266, 397, 491, 921, 985, 1013, 1184
HspAI	GCGC	1 cut(s)	597
Kzo9I	GATC	9 cut(s)	82, 86, 211, 216, 1117, 1151, 1168, 1381, 1438
LmnI	GCTCC	4 cut(s)	34, 301, 937, 1216
Lsp1109I	GCAGC	3 cut(s)	329, 611, 821
LweI	GCATC	1 cut(s)	904
MaeI	CTAG	4 cut(s)	62, 441, 758, 1367
MaeII	ACGT	2 cut(s)	669, 679
MaeIII	GTNAC	1 cut(s)	784
MalI	GATC	9 cut(s)	84, 88, 213, 218, 1119, 1153, 1170, 1383, 1440
MbiI	CCGCTC	1 cut(s)	741
MboI	GATC	9 cut(s)	82, 86, 211, 216, 1117, 1151, 1168, 1381, 1438
MboII	GAAGA	7 cut(s)	10, 125, 167, 626, 746, 916, 1334
MfeI	CAATTG	1 cut(s)	591
MflI	RGATCY	4 cut(s)	211, 1117, 1151, 1168
MhlI	GDGCHC	7 cut(s)	262, 298, 381, 406, 487, 508, 1354
MlsI	TGGCCA	1 cut(s)	143
MluCI	AATT	8 cut(s)	388, 417, 591, 963, 975, 1036, 1134, 1313
MluNI	TGGCCA	1 cut(s)	143
MlyI	GAGTC	3 cut(s)	809, 848, 1011
MmeI	TCCRAC	4 cut(s)	340, 416, 444, 805
Mox20I	TGGCCA	1 cut(s)	143
Mph1103I	ATGCAT	2 cut(s)	399, 919
MroXI	GAANNNNTTC	1 cut(s)	1313
MscI	TGGCCA	1 cut(s)	143
MseI	TTAA	6 cut(s)	74, 167, 416, 426, 1275, 1338
MslI	CAYNNNNRTG	2 cut(s)	267, 1367
Msp20I	TGGCCA	1 cut(s)	143
MspA1I	CMGCKG	1 cut(s)	320
MspCI	CTTAAG	1 cut(s)	1337
MspI	CCGG	3 cut(s)	110, 300, 845
MspR9I	CCNGG	2 cut(s)	145, 300
MunI	CAATTG	1 cut(s)	591
Mva1269I	GAATGC	1 cut(s)	1249
MvaI	CCWGG	1 cut(s)	145
MwoI	GCNNNNNNNGC	5 cut(s)	323, 329, 338, 410, 650
NciI	CCSGG	1 cut(s)	300
NcoI	CCATGG	1 cut(s)	1009
NdeII	GATC	9 cut(s)	82, 86, 211, 216, 1117, 1151, 1168, 1381, 1438
NlaIII	CATG	7 cut(s)	266, 397, 491, 921, 985, 1013, 1184
NlaIV	GGNNCC	6 cut(s)	453, 506, 526, 891, 1119, 1332
NmeAIII	GCCGAG	2 cut(s)	260, 703
NmuCI	GTSAC	1 cut(s)	784
NsiI	ATGCAT	2 cut(s)	399, 919
NspI	RCATGY	1 cut(s)	921
OliI	CACNNNNGTG	1 cut(s)	1367
PaqCI	CACCTGC	1 cut(s)	910
PceI	AGGCCT	1 cut(s)	1358
PcsI	WCGNNNNNNNCGW	1 cut(s)	27
PctI	GAATGC	1 cut(s)	1249
PdmI	GAANNNNTTC	1 cut(s)	1313
PfeI	GAWTC	4 cut(s)	276, 749, 804, 1302
PflMI	CCANNNNNTGG	1 cut(s)	399
PkrI	GCNGC	7 cut(s)	319, 322, 325, 601, 643, 743, 836
Ple19I	CGATCG	1 cut(s)	89
PleI	GAGTC	3 cut(s)	809, 847, 1011
PpsI	GAGTC	3 cut(s)	809, 847, 1011
PpuMI	RGGWCCY	1 cut(s)	1330
Psp5II	RGGWCCY	1 cut(s)	1330
Psp6I	CCWGG	1 cut(s)	143
PspFI	CCCAGC	2 cut(s)	49, 312
PspGI	CCWGG	1 cut(s)	143
PspN4I	GGNNCC	6 cut(s)	453, 506, 526, 891, 1119, 1332
PspOMI	GGGCCC	1 cut(s)	504
PspPI	GGNCC	4 cut(s)	411, 504, 505, 1330
PspPPI	RGGWCCY	1 cut(s)	1330
PstI	CTGCAG	1 cut(s)	793
PstNI	CAGNNNCTG	1 cut(s)	139
PsuI	RGATCY	4 cut(s)	211, 1117, 1151, 1168
PvuI	CGATCG	1 cut(s)	89
RseI	CAYNNNNRTG	2 cut(s)	267, 1367
SaqAI	TTAA	6 cut(s)	74, 167, 416, 426, 1275, 1338
SatI	GCNGC	7 cut(s)	318, 321, 324, 600, 642, 742, 835
Sau3AI	GATC	9 cut(s)	82, 86, 211, 216, 1117, 1151, 1168, 1381, 1438
Sau96I	GGNCC	4 cut(s)	411, 504, 505, 1330
SchI	GAGTC	3 cut(s)	809, 848, 1011
ScrFI	CCNGG	2 cut(s)	145, 300
SduI	GDGCHC	7 cut(s)	262, 298, 381, 406, 487, 508, 1354
SfaNI	GCATC	1 cut(s)	904
SfcI	CTRYAG	2 cut(s)	789, 950
SinI	GGWCC	1 cut(s)	1330
SmiMI	CAYNNNNRTG	2 cut(s)	267, 1367
SmlI	CTYRAG	1 cut(s)	1337
SmoI	CTYRAG	1 cut(s)	1337
Sse9I	AATT	8 cut(s)	388, 417, 591, 963, 975, 1036, 1134, 1313
SseBI	AGGCCT	1 cut(s)	1358
SsiI	CCGC	9 cut(s)	41, 220, 237, 306, 320, 323, 642, 741, 897
SspMI	CTAG	4 cut(s)	62, 441, 758, 1367
StuI	AGGCCT	1 cut(s)	1358
StyD4I	CCNGG	2 cut(s)	143, 298
StyI	CCWWGG	2 cut(s)	1009, 1288
TaaI	ACNGT	4 cut(s)	152, 338, 831, 1142
TaiI	ACGT	2 cut(s)	672, 682
TaqI	TCGA	5 cut(s)	85, 208, 807, 1105, 1416
TasI	AATT	8 cut(s)	388, 417, 591, 963, 975, 1036, 1134, 1313
TauI	GCSGC	4 cut(s)	323, 326, 644, 744
TfiI	GAWTC	4 cut(s)	276, 749, 804, 1302
Tru1I	TTAA	6 cut(s)	74, 167, 416, 426, 1275, 1338
Tru9I	TTAA	6 cut(s)	74, 167, 416, 426, 1275, 1338
TscAI	CASTG	5 cut(s)	274, 341, 793, 836, 1354
TseFI	GTSAC	1 cut(s)	784
TseI	GCWGC	3 cut(s)	317, 599, 834
Tsp45I	GTSAC	1 cut(s)	784
TspDTI	ATGAA	3 cut(s)	623, 627, 642
TspGWI	ACGGA	2 cut(s)	994, 1010
TspRI	CASTG	5 cut(s)	274, 341, 793, 836, 1354
Van91I	CCANNNNNTGG	1 cut(s)	399
Vha464I	CTTAAG	1 cut(s)	1337
VpaK11BI	GGWCC	1 cut(s)	1330
XapI	RAATTY	3 cut(s)	388, 1036, 1313
XceI	RCATGY	1 cut(s)	921
XmiI	GTMKAC	1 cut(s)	1015
XmnI	GAANNNNTTC	1 cut(s)	1313
XspI	CTAG	4 cut(s)	62, 441, 758, 1367
Zsp2I	ATGCAT	2 cut(s)	399, 919

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.