Rmu_sc0000293.1_g000001

kinesin light chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000293.1
OG0002412
Physical Location & Seq
Forward (+)
1 .. 12186
12186 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1535 bp
ttgtaggcggttacaagttttctgatcagcattggaggatggaccctcggctctggttttttctatctggaaatgcagcaattattcttgccataaattccaacaccgtcttggcaaaagatgcatcacttgataatgaggcacaggggatggaaggggatgacacaatgcaattacgcagagttgaggatgcttctgtcaaatctaacatacatacctctaagtggagagtttttacagataaagggagggaattcttccttcagggaaaagtggaggaagctgaaaagctctttctcgcagctttacaggaagctgaagaaggttttgggaagagggatccgcatgttgcatctgcaagcaataatttagcagagctctatagagttaataagctatatcataaggcagagccattgtatttggaggccatcagcatactggaggagtcatttggtcctgaagatatacgagttgggtctgcctttcataacctgggtcagttctatattgtacagcggaagctggatgaagcccgtgtttgctatgagattaaacgccgtgttctgggtcttaaccatacaggttatgcagatacaatgtatcatcttggaacggtgctacacctccaaggaaaagaaaaggactctgaggccctcgttcaagattccattcgcatacttgaggatggtggtcaaggagagtcggttatgtgcttaaagagaatgcgatatcttgctcagatatatctgaaatccaatcgatttgaggaggccgagaaggtacaaagaaagatcctgcatatactggaaataacaaagggatggaatgcattggatacagcaataacagctgaagggctcgcactgatcctccaatccactgggagcttaaaggaagctgaagagcttcttgaaagatgtcttgatgcccgaaaagccttacttcctcaagatcatgttcagattggtgtgaacttgcttcatattgctagagtggcaatgctcaattccgaccaactgaagaatattgactatccaaaagcaatttctgagcttgataaggctaaagaagctttacatgagtccatgaggatagcacaatgttccttggataagttgacgaagcaaaataagaagattcgtagtaatggagcaccaggtgaaattggaaagaatggtcaggtgccattagtcatattgttgcaagcttctgatgctcttggccttttggagatcacaaagaaacaattacaagggtcacagcagtctgagcatgcacctaatatcgaagctgagaatgcactatttaaatgtatatctgcttacaaagagttcgtaactgagagatcaatccctgagtctcctgaaacaaaggccgagtatcttaaatgtttgaagcatcttttgagcttgaatagtgatagcactacagaaagaacacagcactctactggagtcaatttgcaagatctgagggacgaaattaagcgtcttgaagttgaactttctcctcataggaaacgcaaaatttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TPR_10 TPR_12 TPR_7 TPR_8

Protein Analysis

510

Amino Acids

57.55

Weight (kDa)

6.39

Isoelectric Point (pI)

46.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_NPHP3 PF24885 76 - 180 1.3e-08 Nephrocystin-3 TPR domain
TPR_12 PF13424 86 - 149 1.1e-08 Tetratricopeptide repeat
TPR_12 PF13424 116 - 183 2.9e-09 Tetratricopeptide repeat
TPR_MalT PF17874 200 - 329 2.7e-06 MalT-like TPR region
TPR_12 PF13424 244 - 312 8.5e-09 Tetratricopeptide repeat
TPR_10 PF13374 280 - 319 1.4e-06 Tetratricopeptide repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1185
AciI CCGC 3 cut(s) 8, 343, 519
AclWI GGATC 4 cut(s) 334, 347, 789, 864
AcsI RAATTY 3 cut(s) 96, 253, 1529
AcuI CTGAAG 6 cut(s) 247, 338, 482, 875, 923, 1042
AdeI CACNNNGTG 1 cut(s) 1162
AfaI GTAC 2 cut(s) 515, 785
AfiI CCNNNNNNNGG 2 cut(s) 224, 567
AgsI TTSAA 6 cut(s) 664, 916, 1398, 1416, 1498, 1504
AjnI CCWGG 2 cut(s) 494, 1158
AjuI GAANNNNNNNTTGG 4 cut(s) 311, 343, 1032, 1064
Alw21I GWGCWC 2 cut(s) 380, 1158
Alw26I GTCTC 1 cut(s) 1367
AlwI GGATC 4 cut(s) 334, 347, 789, 864
AoxI GGCC 5 cut(s) 428, 653, 773, 1224, 1376
ApeKI GCWGC 2 cut(s) 76, 301
ApoI RAATTY 3 cut(s) 96, 253, 1529
Asp700I GAANNNNTTC 1 cut(s) 908
AspS9I GGNCC 3 cut(s) 42, 457, 654
AsuHPI GGTGA 1 cut(s) 1174
AvaII GGWCC 2 cut(s) 42, 457
BamHI GGATCC 1 cut(s) 339
BanI GGYRCC 1 cut(s) 1185
BanII GRGCYC 2 cut(s) 380, 863
Bbv12I GWGCWC 2 cut(s) 380, 1158
BbvI GCAGC 2 cut(s) 88, 313
BccI CCATC 5 cut(s) 33, 144, 439, 682, 818
BceAI ACGGC 1 cut(s) 545
BciT130I CCWGG 2 cut(s) 496, 1160
BciVI GTATCC 1 cut(s) 831
BclI TGATCA 1 cut(s) 24
BcoDI GTCTC 1 cut(s) 1367
BfaI CTAG 1 cut(s) 992
BfmI CTRYAG 2 cut(s) 381, 1430
BfuI GTATCC 1 cut(s) 831
BglII AGATCT 1 cut(s) 1470
BisI GCNGC 2 cut(s) 77, 302
BlsI GCNGC 2 cut(s) 78, 303
Bme1390I CCNGG 2 cut(s) 496, 1160
Bme18I GGWCC 2 cut(s) 42, 457
BmgT120I GGNCC 3 cut(s) 42, 457, 654
BmiI GGNNCC 3 cut(s) 44, 341, 1187
BmrFI CCNGG 2 cut(s) 496, 1160
BmrI ACTGGG 1 cut(s) 893
BmsI GCATC 7 cut(s) 111, 133, 180, 361, 918, 1206, 1410
BmuI ACTGGG 1 cut(s) 893
BpmI CTGGAG 2 cut(s) 463, 1475
BpuEI CTTGAG 2 cut(s) 703, 935
Bsa29I ATCGAT 1 cut(s) 762
BsaJI CCNNGG 4 cut(s) 46, 495, 630, 1109
BsaXI ACNNNNNCTCC 4 cut(s) 692, 722, 857, 887
Bsc4I CCNNNNNNNGG 2 cut(s) 224, 567
Bse1I ACTGG 4 cut(s) 446, 812, 888, 1458
Bse3DI GCAATG 1 cut(s) 1007
BseBI CCWGG 2 cut(s) 496, 1160
BseCI ATCGAT 1 cut(s) 762
BseDI CCNNGG 4 cut(s) 46, 495, 630, 1109
BseGI GGATG 7 cut(s) 44, 155, 165, 195, 534, 693, 829
BseLI CCNNNNNNNGG 2 cut(s) 224, 567
BseMI GCAATG 1 cut(s) 1007
BseMII CTCAG 8 cut(s) 641, 754, 1043, 1262, 1286, 1334, 1349, 1465
BseNI ACTGG 4 cut(s) 446, 812, 888, 1458
BseRI GAGGAG 3 cut(s) 460, 784, 1502
BseXI GCAGC 2 cut(s) 88, 313
BshFI GGCC 5 cut(s) 430, 655, 775, 1226, 1378
BshNI GGYRCC 1 cut(s) 1185
BshVI ATCGAT 1 cut(s) 762
BsiHKAI GWGCWC 2 cut(s) 380, 1158
BslFI GGGAC 1 cut(s) 1492
BslI CCNNNNNNNGG 2 cut(s) 224, 567
BsmAI GTCTC 1 cut(s) 1367
BsmFI GGGAC 1 cut(s) 1492
BsmI GAATGC 3 cut(s) 731, 834, 1305
BsnI GGCC 5 cut(s) 430, 655, 775, 1226, 1378
Bsp1286I GDGCHC 3 cut(s) 380, 863, 1158
Bsp1407I TGTACA 1 cut(s) 513
Bsp143I GATC 8 cut(s) 24, 339, 794, 869, 954, 1235, 1348, 1470
BspACI CCGC 3 cut(s) 8, 343, 519
BspANI GGCC 5 cut(s) 430, 655, 775, 1226, 1378
BspCNI CTCAG 8 cut(s) 642, 753, 1044, 1263, 1287, 1335, 1350, 1466
BspDI ATCGAT 1 cut(s) 762
BspLI GGNNCC 3 cut(s) 44, 341, 1187
BspPI GGATC 4 cut(s) 334, 347, 789, 864
BspQI GCTCTTC 1 cut(s) 899
BspT107I GGYRCC 1 cut(s) 1185
BsrDI GCAATG 1 cut(s) 1007
BsrGI TGTACA 1 cut(s) 513
BsrI ACTGG 4 cut(s) 446, 812, 888, 1458
BssECI CCNNGG 4 cut(s) 46, 495, 630, 1109
BssMI GATC 8 cut(s) 24, 339, 794, 869, 954, 1235, 1348, 1470
BssT1I CCWWGG 2 cut(s) 630, 1109
Bst2UI CCWGG 2 cut(s) 496, 1160
Bst4CI ACNGT 2 cut(s) 108, 618
Bst6I CTCTTC 2 cut(s) 328, 899
BstAPI GCANNNNNTGC 1 cut(s) 121
BstAUI TGTACA 1 cut(s) 513
BstC8I GCNNGC 4 cut(s) 360, 863, 1208, 1277
BstDEI CTNAG 9 cut(s) 221, 650, 740, 1052, 1271, 1295, 1343, 1358, 1474
BstF5I GGATG 7 cut(s) 44, 155, 165, 195, 534, 693, 829
BstKTI GATC 8 cut(s) 27, 342, 797, 872, 957, 1238, 1351, 1473
BstMAI GTCTC 1 cut(s) 1367
BstMBI GATC 8 cut(s) 24, 339, 794, 869, 954, 1235, 1348, 1470
BstMWI GCNNNNNNNGC 8 cut(s) 121, 850, 937, 997, 1072, 1216, 1272, 1300
BstNI CCWGG 2 cut(s) 496, 1160
BstNSI RCATGY 2 cut(s) 349, 1279
BstSCI CCNGG 2 cut(s) 494, 1158
BstSFI CTRYAG 2 cut(s) 381, 1430
BstV1I GCAGC 2 cut(s) 88, 313
BstX2I RGATCY 3 cut(s) 339, 794, 1470
BstXI CCANNNNNNTGG 1 cut(s) 883
BstYI RGATCY 3 cut(s) 339, 794, 1470
Bsu15I ATCGAT 1 cut(s) 762
BsuI GTATCC 1 cut(s) 831
BsuRI GGCC 5 cut(s) 430, 655, 775, 1226, 1378
BsuTUI ATCGAT 1 cut(s) 762
BtsCI GGATG 7 cut(s) 44, 155, 165, 195, 534, 693, 829
BtsIMutI CAGTG 2 cut(s) 865, 881
Cac8I GCNNGC 4 cut(s) 360, 863, 1208, 1277
Cfr13I GGNCC 3 cut(s) 42, 457, 654
ClaI ATCGAT 1 cut(s) 762
CseI GACGC 1 cut(s) 1480
CsiI ACCWGGT 1 cut(s) 1158
Csp6I GTAC 2 cut(s) 514, 784
CviAII CATG 5 cut(s) 346, 958, 1081, 1089, 1276
CviQI GTAC 2 cut(s) 514, 784
DdeI CTNAG 9 cut(s) 221, 650, 740, 1052, 1271, 1295, 1343, 1358, 1474
DpnI GATC 8 cut(s) 26, 341, 796, 871, 956, 1237, 1350, 1472
DpnII GATC 8 cut(s) 24, 339, 794, 869, 954, 1235, 1348, 1470
DraI TTTAAA 1 cut(s) 1311
DraIII CACNNNGTG 1 cut(s) 1162
Eam1104I CTCTTC 2 cut(s) 328, 899
EarI CTCTTC 2 cut(s) 328, 899
Ecl136II GAGCTC 1 cut(s) 378
Eco130I CCWWGG 2 cut(s) 630, 1109
Eco24I GRGCYC 2 cut(s) 380, 863
Eco32I GATATC 1 cut(s) 733
Eco47I GGWCC 2 cut(s) 42, 457
Eco53kI GAGCTC 1 cut(s) 378
Eco57I CTGAAG 6 cut(s) 247, 338, 482, 875, 923, 1042
EcoICRI GAGCTC 1 cut(s) 378
EcoO109I RGGNCCY 1 cut(s) 654
EcoRI GAATTC 1 cut(s) 253
EcoRII CCWGG 2 cut(s) 494, 1158
EcoRV GATATC 1 cut(s) 733
EcoT14I CCWWGG 2 cut(s) 630, 1109
EcoT22I ATGCAT 2 cut(s) 126, 834
EcoT38I GRGCYC 2 cut(s) 380, 863
ErhI CCWWGG 2 cut(s) 630, 1109
FaeI CATG 5 cut(s) 349, 961, 1084, 1092, 1279
FalI AAGNNNNNCTT 6 cut(s) 896, 928, 929, 961, 1490, 1522
FaqI GGGAC 1 cut(s) 1492
FatI CATG 5 cut(s) 345, 957, 1080, 1088, 1275
FbaI TGATCA 1 cut(s) 24
Fnu4HI GCNGC 2 cut(s) 77, 302
FokI GGATG 7 cut(s) 51, 162, 172, 202, 541, 700, 836
FriOI GRGCYC 2 cut(s) 380, 863
Fsp4HI GCNGC 2 cut(s) 77, 302
FspBI CTAG 1 cut(s) 992
GluI GCNGC 2 cut(s) 77, 302
GsuI CTGGAG 2 cut(s) 463, 1475
HaeIII GGCC 5 cut(s) 430, 655, 775, 1226, 1378
HgaI GACGC 1 cut(s) 1480
Hin1II CATG 5 cut(s) 349, 961, 1084, 1092, 1279
HincII GTYRAC 1 cut(s) 1121
HindII GTYRAC 1 cut(s) 1121
HindIII AAGCTT 2 cut(s) 1073, 1208
HinfI GANTC 8 cut(s) 448, 646, 667, 703, 1084, 1140, 1360, 1457
HphI GGTGA 1 cut(s) 1174
Hpy166II GTNNAC 2 cut(s) 975, 1121
Hpy188III TCNNGA 8 cut(s) 68, 460, 664, 913, 925, 952, 1366, 1495
Hpy8I GTNNAC 2 cut(s) 975, 1121
HpyAV CCTTC 5 cut(s) 148, 271, 316, 774, 850
HpyCH4III ACNGT 2 cut(s) 108, 618
HpyF10VI GCNNNNNNNGC 8 cut(s) 121, 850, 937, 997, 1072, 1216, 1272, 1300
HpyF3I CTNAG 9 cut(s) 221, 650, 740, 1052, 1271, 1295, 1343, 1358, 1474
Hsp92II CATG 5 cut(s) 349, 961, 1084, 1092, 1279
Ksp22I TGATCA 1 cut(s) 24
Kzo9I GATC 8 cut(s) 24, 339, 794, 869, 954, 1235, 1348, 1470
LguI GCTCTTC 1 cut(s) 899
LmnI GCTCC 2 cut(s) 887, 1153
Lsp1109I GCAGC 2 cut(s) 88, 313
LweI GCATC 7 cut(s) 111, 133, 180, 361, 918, 1206, 1410
MabI ACCWGGT 1 cut(s) 1158
MaeI CTAG 1 cut(s) 992
MaeIII GTNAC 3 cut(s) 10, 1259, 1338
MalI GATC 8 cut(s) 26, 341, 796, 871, 956, 1237, 1350, 1472
MboI GATC 8 cut(s) 24, 339, 794, 869, 954, 1235, 1348, 1470
MboII GAAGA 7 cut(s) 249, 331, 345, 475, 916, 1035, 1149
MflI RGATCY 3 cut(s) 339, 794, 1470
MhlI GDGCHC 3 cut(s) 380, 863, 1158
MlyI GAGTC 6 cut(s) 457, 640, 712, 1093, 1369, 1466
MmeI TCCRAC 2 cut(s) 125, 1037
Mph1103I ATGCAT 2 cut(s) 126, 834
MroXI GAANNNNTTC 1 cut(s) 908
MseI TTAA 8 cut(s) 389, 554, 575, 718, 892, 1310, 1388, 1487
MspA1I CMGCKG 2 cut(s) 519, 853
MspR9I CCNGG 2 cut(s) 496, 1160
Mva1269I GAATGC 3 cut(s) 731, 834, 1305
MvaI CCWGG 2 cut(s) 496, 1160
MwoI GCNNNNNNNGC 8 cut(s) 121, 850, 937, 997, 1072, 1216, 1272, 1300
NdeII GATC 8 cut(s) 24, 339, 794, 869, 954, 1235, 1348, 1470
NlaIII CATG 5 cut(s) 349, 961, 1084, 1092, 1279
NlaIV GGNNCC 3 cut(s) 44, 341, 1187
NmeAIII GCCGAG 3 cut(s) 27, 801, 1404
NmuCI GTSAC 1 cut(s) 1259
NsiI ATGCAT 2 cut(s) 126, 834
NspI RCATGY 2 cut(s) 349, 1279
PaeI GCATGC 1 cut(s) 1279
PciSI GCTCTTC 1 cut(s) 899
PctI GAATGC 3 cut(s) 731, 834, 1305
PdmI GAANNNNTTC 1 cut(s) 908
PfeI GAWTC 2 cut(s) 667, 1140
PkrI GCNGC 2 cut(s) 78, 303
PleI GAGTC 6 cut(s) 456, 640, 711, 1092, 1368, 1465
PpsI GAGTC 6 cut(s) 456, 640, 711, 1092, 1368, 1465
Psp124BI GAGCTC 1 cut(s) 380
Psp6I CCWGG 2 cut(s) 494, 1158
PspGI CCWGG 2 cut(s) 494, 1158
PspN4I GGNNCC 3 cut(s) 44, 341, 1187
PspPI GGNCC 3 cut(s) 42, 457, 654
PsuI RGATCY 3 cut(s) 339, 794, 1470
PvuII CAGCTG 1 cut(s) 853
RsaI GTAC 2 cut(s) 515, 785
RsaNI GTAC 2 cut(s) 514, 784
SacI GAGCTC 1 cut(s) 380
SapI GCTCTTC 1 cut(s) 899
SaqAI TTAA 8 cut(s) 389, 554, 575, 718, 892, 1310, 1388, 1487
SatI GCNGC 2 cut(s) 77, 302
Sau3AI GATC 8 cut(s) 24, 339, 794, 869, 954, 1235, 1348, 1470
Sau96I GGNCC 3 cut(s) 42, 457, 654
SchI GAGTC 6 cut(s) 457, 640, 712, 1093, 1369, 1466
ScrFI CCNGG 2 cut(s) 496, 1160
SduI GDGCHC 3 cut(s) 380, 863, 1158
SexAI ACCWGGT 1 cut(s) 1158
SfaNI GCATC 7 cut(s) 111, 133, 180, 361, 918, 1206, 1410
SfcI CTRYAG 2 cut(s) 381, 1430
SinI GGWCC 2 cut(s) 42, 457
SmiI ATTTAAAT 1 cut(s) 1311
SmlI CTYRAG 2 cut(s) 682, 950
SmoI CTYRAG 2 cut(s) 682, 950
SphI GCATGC 1 cut(s) 1279
SsiI CCGC 3 cut(s) 8, 343, 519
SspI AATATT 1 cut(s) 1029
SspMI CTAG 1 cut(s) 992
SstI GAGCTC 1 cut(s) 380
StyD4I CCNGG 2 cut(s) 494, 1158
StyI CCWWGG 2 cut(s) 630, 1109
SwaI ATTTAAAT 1 cut(s) 1311
TaaI ACNGT 2 cut(s) 108, 618
TaqI TCGA 2 cut(s) 762, 1289
TatI WGTACW 1 cut(s) 513
TfiI GAWTC 2 cut(s) 667, 1140
Tru1I TTAA 8 cut(s) 389, 554, 575, 718, 892, 1310, 1388, 1487
Tru9I TTAA 8 cut(s) 389, 554, 575, 718, 892, 1310, 1388, 1487
TscAI CASTG 2 cut(s) 872, 888
TseFI GTSAC 1 cut(s) 1259
TseI GCWGC 2 cut(s) 76, 301
Tsp45I GTSAC 1 cut(s) 1259
TspDTI ATGAA 3 cut(s) 478, 545, 973
TspRI CASTG 2 cut(s) 872, 888
VpaK11BI GGWCC 2 cut(s) 42, 457
XapI RAATTY 3 cut(s) 96, 253, 1529
XceI RCATGY 2 cut(s) 349, 1279
XcmI CCANNNNNNNNNTGG 2 cut(s) 108, 438
XmnI GAANNNNTTC 1 cut(s) 908
XspI CTAG 1 cut(s) 992
Zsp2I ATGCAT 2 cut(s) 126, 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.