Rmu_sc0000295.1_g000006

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000295.1
OG0010577
Physical Location & Seq
Forward (+)
29154 .. 30158
1005 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1005 bp
atgctctatcttccttctcaaaccccggacgaagacatgttgatgttggccttattgttgggttggagaaaagcttcaaagtgcaagaaactactgaaacaagttcgttcccggctcgagtgcttgaaaaacgagaggtctgctatttctacgcagttacggaaagagcttgagaaactagacgacgaggcactgaacctcaggatcgaacaagaacaacacattagagacgaatcttcggtttacaatttgctcgaccatttctgcaacctcatccttaagcactccttttataattatattcgaacgcacaagcagtgtcgtcaaagaaacctagctgaagcagtttcaagtcttgtctttgcttcttcaagattaagatgcggggtgattccagagcttgagcagatcagggacctttttggagagcgttacggggagagttttgtagaaactactgtgaaactggtgcctgggaatcttgtaaaccctaccttcaaaaagactattcttgctctcttcccagactcagtggtgcccaatgatagagtaaaacaagaactggtaaaaccctcttttactagttcttctactgatgaggcaccagaaagatcatcaacagcagaggaaaaggacgacgaggatcgcaacaataaactacaacttattaccttccaatcttctaaacatgtccaccccaaattgcctgactgggatgatatatttaccaagttcagagccctcaagaagcctaaatacgtcaaccctaagttgccagaatatgatgatattacagccaaggataatgctgtcaagagccctattattaataatgagaacactactgctgataattaccgctcgcaattagagtacgatgatgagcatgataatattgatgacggagcaatgttcagttattcatggttgtcgatggagagcattttggcaatgataaaccagccccaatgtgccagtgtcattaacacaatcaccccatattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

334

Amino Acids

38.58

Weight (kDa)

5.76

Isoelectric Point (pI)

48.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 29 - 170 5.9e-22 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 294
AccB1I GGYRCC 3 cut(s) 469, 535, 601
AccBSI CCGCTC 1 cut(s) 861
AciI CCGC 2 cut(s) 384, 859
AclWI GGATC 2 cut(s) 212, 651
AcuI CTGAAG 1 cut(s) 360
AfaI GTAC 1 cut(s) 875
AflII CTTAAG 1 cut(s) 278
AflIII ACRYGT 2 cut(s) 36, 688
AgsI TTSAA 5 cut(s) 78, 127, 351, 372, 499
AhlI ACTAGT 1 cut(s) 581
AjnI CCWGG 1 cut(s) 472
AluBI AGCT 4 cut(s) 74, 169, 338, 400
AluI AGCT 4 cut(s) 74, 169, 338, 400
Alw26I GTCTC 1 cut(s) 222
AlwI GGATC 2 cut(s) 212, 651
Ama87I CYCGRG 1 cut(s) 116
AoxI GGCC 1 cut(s) 48
AseI ATTAAT 1 cut(s) 828
Asp700I GAANNNNTTC 1 cut(s) 73
AspS9I GGNCC 1 cut(s) 415
AsuC2I CCSGG 2 cut(s) 26, 112
AsuHPI GGTGA 2 cut(s) 400, 985
AsuII TTCGAA 1 cut(s) 304
AvaI CYCGRG 1 cut(s) 116
AvaII GGWCC 1 cut(s) 415
AxyI CCTNAGG 1 cut(s) 200
BaeGI GKGCMC 1 cut(s) 540
BanI GGYRCC 3 cut(s) 469, 535, 601
BanII GRGCYC 2 cut(s) 742, 821
BarI GAAGNNNNNNTAC 2 cut(s) 740, 772
BbsI GAAGAC 1 cut(s) 39
BccI CCATC 1 cut(s) 928
BcgI CGANNNNNNTGC 2 cut(s) 122, 156
BciT130I CCWGG 1 cut(s) 474
BcnI CCSGG 2 cut(s) 26, 112
BcoDI GTCTC 1 cut(s) 222
BcuI ACTAGT 1 cut(s) 581
BfaI CTAG 3 cut(s) 179, 335, 582
BfrI CTTAAG 1 cut(s) 278
Bme1390I CCNGG 3 cut(s) 26, 112, 474
Bme18I GGWCC 1 cut(s) 415
BmeT110I CYCGRG 1 cut(s) 116
BmgT120I GGNCC 1 cut(s) 415
BmiI GGNNCC 4 cut(s) 416, 471, 537, 603
BmrFI CCNGG 3 cut(s) 26, 112, 474
BmrI ACTGGG 1 cut(s) 721
BmsI GCATC 1 cut(s) 371
BmuI ACTGGG 1 cut(s) 721
BpiI GAAGAC 1 cut(s) 39
Bpu14I TTCGAA 1 cut(s) 304
BpuEI CTTGAG 3 cut(s) 191, 422, 728
BpuMI CCSGG 2 cut(s) 26, 112
BsaJI CCNNGG 3 cut(s) 24, 473, 798
Bse1I ACTGG 4 cut(s) 471, 567, 716, 975
Bse21I CCTNAGG 1 cut(s) 200
Bse3DI GCAATG 2 cut(s) 915, 957
BseBI CCWGG 1 cut(s) 474
BseDI CCNNGG 3 cut(s) 24, 473, 798
BseGI GGATG 2 cut(s) 273, 721
BseMI GCAATG 2 cut(s) 915, 957
BseMII CTCAG 2 cut(s) 214, 543
BseNI ACTGG 4 cut(s) 471, 567, 716, 975
BseSI GKGCMC 1 cut(s) 540
BshFI GGCC 1 cut(s) 50
BshNI GGYRCC 3 cut(s) 469, 535, 601
BsiHKCI CYCGRG 1 cut(s) 116
BsiSI CCGG 2 cut(s) 26, 112
BslFI GGGAC 1 cut(s) 428
BsmAI GTCTC 1 cut(s) 222
BsmBI CGTCTC 1 cut(s) 222
BsmFI GGGAC 1 cut(s) 428
BsnI GGCC 1 cut(s) 50
BsoBI CYCGRG 1 cut(s) 116
Bsp119I TTCGAA 1 cut(s) 304
Bsp1286I GDGCHC 3 cut(s) 540, 742, 821
Bsp143I GATC 4 cut(s) 204, 408, 611, 643
BspACI CCGC 2 cut(s) 384, 859
BspANI GGCC 1 cut(s) 50
BspCNI CTCAG 2 cut(s) 213, 542
BspLI GGNNCC 4 cut(s) 416, 471, 537, 603
BspPI GGATC 2 cut(s) 212, 651
BspT104I TTCGAA 1 cut(s) 304
BspT107I GGYRCC 3 cut(s) 469, 535, 601
BspTI CTTAAG 1 cut(s) 278
BsrBI CCGCTC 1 cut(s) 861
BsrDI GCAATG 2 cut(s) 915, 957
BsrI ACTGG 4 cut(s) 471, 567, 716, 975
BssECI CCNNGG 3 cut(s) 24, 473, 798
BssMI GATC 4 cut(s) 204, 408, 611, 643
BssT1I CCWWGG 1 cut(s) 798
Bst2UI CCWGG 1 cut(s) 474
Bst4CI ACNGT 1 cut(s) 460
Bst6I CTCTTC 1 cut(s) 524
BstAFI CTTAAG 1 cut(s) 278
BstBI TTCGAA 1 cut(s) 304
BstC8I GCNNGC 1 cut(s) 863
BstDEI CTNAG 3 cut(s) 200, 529, 768
BstF5I GGATG 2 cut(s) 273, 721
BstKTI GATC 4 cut(s) 207, 411, 614, 646
BstMAI GTCTC 1 cut(s) 222
BstMBI GATC 4 cut(s) 204, 408, 611, 643
BstNI CCWGG 1 cut(s) 474
BstNSI RCATGY 2 cut(s) 40, 692
BstSCI CCNGG 3 cut(s) 24, 110, 472
BstSLI GKGCMC 1 cut(s) 540
BstV2I GAAGAC 1 cut(s) 39
Bsu36I CCTNAGG 1 cut(s) 200
BsuRI GGCC 1 cut(s) 50
BtsCI GGATG 2 cut(s) 273, 721
BtsI GCAGTG 1 cut(s) 323
BtsIMutI CAGTG 4 cut(s) 191, 323, 537, 982
Cac8I GCNNGC 1 cut(s) 863
Cfr13I GGNCC 1 cut(s) 415
Csp6I GTAC 1 cut(s) 874
CviAII CATG 4 cut(s) 37, 689, 887, 924
CviQI GTAC 1 cut(s) 874
DdeI CTNAG 3 cut(s) 200, 529, 768
DpnI GATC 4 cut(s) 206, 410, 613, 645
DpnII GATC 4 cut(s) 204, 408, 611, 643
Eam1104I CTCTTC 1 cut(s) 524
EarI CTCTTC 1 cut(s) 524
Eco130I CCWWGG 1 cut(s) 798
Eco24I GRGCYC 2 cut(s) 742, 821
Eco47I GGWCC 1 cut(s) 415
Eco57I CTGAAG 1 cut(s) 360
Eco81I CCTNAGG 1 cut(s) 200
Eco88I CYCGRG 1 cut(s) 116
EcoO109I RGGNCCY 1 cut(s) 415
EcoRII CCWGG 1 cut(s) 472
EcoT14I CCWWGG 1 cut(s) 798
EcoT38I GRGCYC 2 cut(s) 742, 821
ErhI CCWWGG 1 cut(s) 798
Esp3I CGTCTC 1 cut(s) 222
FaeI CATG 4 cut(s) 40, 692, 890, 927
FaiI YATR 9 cut(s) 38, 294, 300, 690, 722, 783, 888, 925, 1000
FalI AAGNNNNNCTT 2 cut(s) 272, 304
FaqI GGGAC 1 cut(s) 428
FatI CATG 4 cut(s) 36, 688, 886, 923
FauI CCCGC 1 cut(s) 377
FokI GGATG 2 cut(s) 260, 728
FriOI GRGCYC 2 cut(s) 742, 821
FspBI CTAG 3 cut(s) 179, 335, 582
HaeIII GGCC 1 cut(s) 50
HapII CCGG 2 cut(s) 26, 112
Hin1II CATG 4 cut(s) 40, 692, 890, 927
HincII GTYRAC 1 cut(s) 763
HindII GTYRAC 1 cut(s) 763
HindIII AAGCTT 1 cut(s) 72
HinfI GANTC 4 cut(s) 233, 391, 478, 527
HpaII CCGG 2 cut(s) 26, 112
HphI GGTGA 2 cut(s) 400, 985
Hpy166II GTNNAC 4 cut(s) 244, 487, 694, 763
Hpy188I TCNGA 1 cut(s) 737
Hpy188III TCNNGA 5 cut(s) 202, 372, 395, 745, 814
Hpy8I GTNNAC 4 cut(s) 244, 487, 694, 763
Hpy99I CGWCG 2 cut(s) 188, 641
HpyAV CCTTC 3 cut(s) 24, 505, 682
HpyCH4III ACNGT 1 cut(s) 460
HpyCH4IV ACGT 1 cut(s) 759
HpyCH4V TGCA 2 cut(s) 84, 267
HpyF3I CTNAG 3 cut(s) 200, 529, 768
HpySE526I ACGT 1 cut(s) 759
Hsp92II CATG 4 cut(s) 40, 692, 890, 927
Kzo9I GATC 4 cut(s) 204, 408, 611, 643
LmnI GCTCC 1 cut(s) 905
LweI GCATC 1 cut(s) 371
MaeI CTAG 3 cut(s) 179, 335, 582
MaeII ACGT 1 cut(s) 759
MaeIII GTNAC 2 cut(s) 156, 431
MalI GATC 4 cut(s) 206, 410, 613, 645
MbiI CCGCTC 1 cut(s) 861
MboI GATC 4 cut(s) 204, 408, 611, 643
MboII GAAGA 6 cut(s) 44, 228, 360, 511, 579, 672
MhlI GDGCHC 3 cut(s) 540, 742, 821
MluCI AATT 5 cut(s) 247, 295, 701, 853, 866
MlyI GAGTC 1 cut(s) 521
MmeI TCCRAC 1 cut(s) 44
MnlI CCTC 9 cut(s) 129, 181, 209, 281, 583, 592, 619, 634, 752
MroXI GAANNNNTTC 1 cut(s) 73
MseI TTAA 4 cut(s) 279, 377, 828, 984
MslI CAYNNNNRTG 1 cut(s) 41
MspCI CTTAAG 1 cut(s) 278
MspI CCGG 2 cut(s) 26, 112
MspR9I CCNGG 3 cut(s) 26, 112, 474
MvaI CCWGG 1 cut(s) 474
NciI CCSGG 2 cut(s) 26, 112
NdeII GATC 4 cut(s) 204, 408, 611, 643
NlaIII CATG 4 cut(s) 40, 692, 890, 927
NlaIV GGNNCC 4 cut(s) 416, 471, 537, 603
NspI RCATGY 2 cut(s) 40, 692
NspV TTCGAA 1 cut(s) 304
PaeR7I CTCGAG 1 cut(s) 116
PciI ACATGT 2 cut(s) 36, 688
PdmI GAANNNNTTC 1 cut(s) 73
PfeI GAWTC 3 cut(s) 233, 391, 478
PleI GAGTC 1 cut(s) 521
PpsI GAGTC 1 cut(s) 521
PpuMI RGGWCCY 1 cut(s) 415
PscI ACATGT 2 cut(s) 36, 688
PshBI ATTAAT 1 cut(s) 828
PsiI TTATAA 1 cut(s) 294
Psp5II RGGWCCY 1 cut(s) 415
Psp6I CCWGG 1 cut(s) 472
PspGI CCWGG 1 cut(s) 472
PspN4I GGNNCC 4 cut(s) 416, 471, 537, 603
PspPI GGNCC 1 cut(s) 415
PspPPI RGGWCCY 1 cut(s) 415
PspXI VCTCGAGB 1 cut(s) 116
RsaI GTAC 1 cut(s) 875
RsaNI GTAC 1 cut(s) 874
RseI CAYNNNNRTG 1 cut(s) 41
SaqAI TTAA 4 cut(s) 279, 377, 828, 984
Sau3AI GATC 4 cut(s) 204, 408, 611, 643
Sau96I GGNCC 1 cut(s) 415
SchI GAGTC 1 cut(s) 521
ScrFI CCNGG 3 cut(s) 26, 112, 474
SduI GDGCHC 3 cut(s) 540, 742, 821
SfaNI GCATC 1 cut(s) 371
Sfr274I CTCGAG 1 cut(s) 116
SfuI TTCGAA 1 cut(s) 304
SinI GGWCC 1 cut(s) 415
SlaI CTCGAG 1 cut(s) 116
SmiMI CAYNNNNRTG 1 cut(s) 41
SmlI CTYRAG 5 cut(s) 116, 170, 278, 401, 743
SmoI CTYRAG 5 cut(s) 116, 170, 278, 401, 743
SpeI ACTAGT 1 cut(s) 581
Sse9I AATT 5 cut(s) 247, 295, 701, 853, 866
SsiI CCGC 2 cut(s) 384, 859
SspI AATATT 1 cut(s) 895
SspMI CTAG 3 cut(s) 179, 335, 582
StyD4I CCNGG 3 cut(s) 24, 110, 472
StyI CCWWGG 1 cut(s) 798
TaaI ACNGT 1 cut(s) 460
TaiI ACGT 1 cut(s) 762
TaqI TCGA 5 cut(s) 117, 207, 255, 304, 932
TasI AATT 5 cut(s) 247, 295, 701, 853, 866
TfiI GAWTC 3 cut(s) 233, 391, 478
Tru1I TTAA 4 cut(s) 279, 377, 828, 984
Tru9I TTAA 4 cut(s) 279, 377, 828, 984
TscAI CASTG 4 cut(s) 198, 323, 537, 982
TspDTI ATGAA 1 cut(s) 912
TspGWI ACGGA 2 cut(s) 175, 918
TspRI CASTG 4 cut(s) 198, 323, 537, 982
Vha464I CTTAAG 1 cut(s) 278
VpaK11BI GGWCC 1 cut(s) 415
VspI ATTAAT 1 cut(s) 828
XceI RCATGY 2 cut(s) 40, 692
XhoI CTCGAG 1 cut(s) 116
XmnI GAANNNNTTC 1 cut(s) 73
XspI CTAG 3 cut(s) 179, 335, 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.