Rmu_sc0000295.1_g000044

Regulator of Vps4 activity in the MVB pathway

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000295.1
OG0010577
Physical Location & Seq
Reverse (-)
234955 .. 237096
2142 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 993 bp
atgctttcctgctccatccgcgaatgttcgctaaagtgtgttcgcaagatgaaatcactgcggagaaaagttcaccaagtgagttcgccgcgaaggttcgctaaagtgcgttcgcgagatgacgtcattggggggaatagtaagaaagggttagggaaagatgttgaggagctagactacaaggcactgaacctcagggtcgaacaggaacagcatatcagagacgaatcttcggtgtacactttgctcgaccatttctgcaagctcatcctcatgcacttcttttatatacgaacgcacaggcaatgtcgtcaaagagacctagctgtagcagtttcaagtcttgtctttgcttcttcaagcttaggacgtggggtgattcctgagtttgaggagatcagggacttactcggagagcgttacggggagagttttgttgaaactgctgtgaagttgttgcctgggaatcttgtaaaccctaacttccagaagaatattctcgctctctccccagaaacagtggtgcctaatgatatagtaaaacatgaactggttaaaccctcttttactacttcttctcctgatgaggcaccagaaagatcatcagcagagaaaaaggacgacgagaatcggaacaatgaattacaacttatcagcttacaatctcctatgcatgtccaccccaaattgcctgattgggatgatatatttactaagttcagtgctctcaagaagcccaagtatttgcaccctaagttgccggaatatgatgatattacagccaagtttaatgctctcaagatccccatgaatgagaaccctcctaataactaccagttgcaattagagtaccatgacgatcatgacaatattgatgatagagccatattcgactattccaggctctcgattgagagcagctgcaatttgataagcccgtccccaatgttgttattaacgcaatcaccccatattgaatacctagcttattag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04144 map04144
Pfam Domains
Protein Families
Ist1

Protein Analysis

330

Amino Acids

37.98

Weight (kDa)

6.64

Isoelectric Point (pI)

61.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ist1 PF03398 62 - 172 2.5e-16 Regulator of Vps4 activity in the MVB pathway
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 126
AccB1I GGYRCC 2 cut(s) 523, 589
AccII CGCG 3 cut(s) 21, 91, 115
AciI CCGC 3 cut(s) 19, 61, 89
AclWI GGATC 1 cut(s) 796
AcyI GRCGYC 1 cut(s) 123
AdeI CACNNNGTG 1 cut(s) 79
AfaI GTAC 2 cut(s) 239, 851
AgsI TTSAA 4 cut(s) 339, 360, 440, 977
AjiI CACGTC 1 cut(s) 371
AjnI CCWGG 2 cut(s) 460, 899
AluBI AGCT 7 cut(s) 172, 265, 326, 363, 657, 921, 986
AluI AGCT 7 cut(s) 172, 265, 326, 363, 657, 921, 986
Alw21I GWGCWC 1 cut(s) 727
Alw26I GTCTC 2 cut(s) 216, 312
AlwI GGATC 1 cut(s) 796
ApeKI GCWGC 2 cut(s) 918, 921
AsuHPI GGTGA 3 cut(s) 65, 388, 957
AxyI CCTNAGG 1 cut(s) 194
BanI GGYRCC 2 cut(s) 523, 589
Bbv12I GWGCWC 1 cut(s) 727
BbvI GCAGC 2 cut(s) 908, 930
BccI CCATC 1 cut(s) 23
BciT130I CCWGG 2 cut(s) 462, 901
BcoDI GTCTC 2 cut(s) 216, 312
BfaI CTAG 3 cut(s) 173, 323, 983
BfmI CTRYAG 1 cut(s) 327
BisI GCNGC 3 cut(s) 89, 919, 922
BlsI GCNGC 3 cut(s) 90, 920, 923
Bme1390I CCNGG 2 cut(s) 462, 901
BmgBI CACGTC 1 cut(s) 371
BmiI GGNNCC 2 cut(s) 525, 591
BmrFI CCNGG 2 cut(s) 462, 901
Bpu10I CCTNAGC 1 cut(s) 364
BpuEI CTTGAG 2 cut(s) 713, 782
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 312
BsaJI CCNNGG 1 cut(s) 461
BsaXI ACNNNNNCTCC 2 cut(s) 562, 592
Bse1I ACTGG 2 cut(s) 555, 835
Bse21I CCTNAGG 1 cut(s) 194
Bse3DI GCAATG 1 cut(s) 311
BseBI CCWGG 2 cut(s) 462, 901
BseDI CCNNGG 1 cut(s) 461
BseGI GGATG 3 cut(s) 15, 267, 706
BseMI GCAATG 1 cut(s) 311
BseMII CTCAG 2 cut(s) 208, 375
BseNI ACTGG 2 cut(s) 555, 835
BseRI GAGGAG 2 cut(s) 182, 407
BseXI GCAGC 2 cut(s) 908, 930
Bsh1236I CGCG 3 cut(s) 21, 91, 115
BshNI GGYRCC 2 cut(s) 523, 589
BsiHKAI GWGCWC 1 cut(s) 727
BsiSI CCGG 1 cut(s) 761
BslFI GGGAC 2 cut(s) 416, 925
BsmAI GTCTC 2 cut(s) 216, 312
BsmBI CGTCTC 1 cut(s) 216
BsmFI GGGAC 2 cut(s) 416, 925
Bso31I GGTCTC 1 cut(s) 312
Bsp1286I GDGCHC 1 cut(s) 727
Bsp1407I TGTACA 1 cut(s) 237
Bsp143I GATC 4 cut(s) 396, 599, 801, 859
Bsp68I TCGCGA 1 cut(s) 115
BspACI CCGC 3 cut(s) 19, 61, 89
BspCNI CTCAG 2 cut(s) 207, 376
BspFNI CGCG 3 cut(s) 21, 91, 115
BspHI TCATGA 1 cut(s) 862
BspLI GGNNCC 2 cut(s) 525, 591
BspPI GGATC 1 cut(s) 796
BspT107I GGYRCC 2 cut(s) 523, 589
BspTNI GGTCTC 1 cut(s) 312
BsrDI GCAATG 1 cut(s) 311
BsrGI TGTACA 1 cut(s) 237
BsrI ACTGG 2 cut(s) 555, 835
BssECI CCNNGG 1 cut(s) 461
BssMI GATC 4 cut(s) 396, 599, 801, 859
BssNI GRCGYC 1 cut(s) 123
Bst2UI CCWGG 2 cut(s) 462, 901
Bst4CI ACNGT 1 cut(s) 520
BstACI GRCGYC 1 cut(s) 123
BstAUI TGTACA 1 cut(s) 237
BstC8I GCNNGC 1 cut(s) 263
BstDEI CTNAG 5 cut(s) 194, 364, 384, 714, 753
BstF5I GGATG 3 cut(s) 15, 267, 706
BstFNI CGCG 3 cut(s) 21, 91, 115
BstKTI GATC 4 cut(s) 399, 602, 804, 862
BstMAI GTCTC 2 cut(s) 216, 312
BstMBI GATC 4 cut(s) 396, 599, 801, 859
BstMWI GCNNNNNNNGC 1 cut(s) 18
BstNI CCWGG 2 cut(s) 462, 901
BstNSI RCATGY 1 cut(s) 677
BstSCI CCNGG 2 cut(s) 460, 899
BstSFI CTRYAG 1 cut(s) 327
BstUI CGCG 3 cut(s) 21, 91, 115
BstV1I GCAGC 2 cut(s) 908, 930
BstX2I RGATCY 1 cut(s) 801
BstYI RGATCY 1 cut(s) 801
Bsu36I CCTNAGG 1 cut(s) 194
BtrI CACGTC 1 cut(s) 371
BtsCI GGATG 3 cut(s) 15, 267, 706
BtsI GCAGTG 1 cut(s) 56
BtsIMutI CAGTG 4 cut(s) 56, 185, 525, 727
BtuMI TCGCGA 1 cut(s) 115
Cac8I GCNNGC 1 cut(s) 263
CciI TCATGA 1 cut(s) 862
Csp6I GTAC 2 cut(s) 238, 850
CviAII CATG 6 cut(s) 274, 545, 674, 808, 854, 863
CviQI GTAC 2 cut(s) 238, 850
DdeI CTNAG 5 cut(s) 194, 364, 384, 714, 753
DpnI GATC 4 cut(s) 398, 601, 803, 861
DpnII GATC 4 cut(s) 396, 599, 801, 859
DraIII CACNNNGTG 1 cut(s) 79
Eco31I GGTCTC 1 cut(s) 312
Eco81I CCTNAGG 1 cut(s) 194
EcoRII CCWGG 2 cut(s) 460, 899
EcoT22I ATGCAT 1 cut(s) 675
Esp3I CGTCTC 1 cut(s) 216
FaeI CATG 6 cut(s) 277, 548, 677, 811, 857, 866
FaqI GGGAC 2 cut(s) 416, 925
FatI CATG 6 cut(s) 273, 544, 673, 807, 853, 862
Fnu4HI GCNGC 3 cut(s) 89, 919, 922
FokI GGATG 3 cut(s) 2, 254, 713
Fsp4HI GCNGC 3 cut(s) 89, 919, 922
FspBI CTAG 3 cut(s) 173, 323, 983
GluI GCNGC 3 cut(s) 89, 919, 922
HapII CCGG 1 cut(s) 761
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 6 cut(s) 277, 548, 677, 811, 857, 866
HindIII AAGCTT 1 cut(s) 361
HinfI GANTC 4 cut(s) 227, 379, 466, 628
HpaII CCGG 1 cut(s) 761
HphI GGTGA 3 cut(s) 65, 388, 957
Hpy166II GTNNAC 5 cut(s) 73, 238, 240, 475, 679
Hpy188I TCNGA 3 cut(s) 221, 413, 633
Hpy188III TCNNGA 8 cut(s) 114, 383, 487, 581, 730, 799, 863, 907
Hpy8I GTNNAC 5 cut(s) 73, 238, 240, 475, 679
Hpy99I CGWCG 1 cut(s) 626
HpyAV CCTTC 1 cut(s) 87
HpyCH4III ACNGT 1 cut(s) 520
HpyCH4IV ACGT 2 cut(s) 123, 370
HpyCH4V TGCA 6 cut(s) 261, 277, 673, 748, 841, 924
HpyF10VI GCNNNNNNNGC 1 cut(s) 18
HpyF3I CTNAG 5 cut(s) 194, 364, 384, 714, 753
HpySE526I ACGT 2 cut(s) 123, 370
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 6 cut(s) 277, 548, 677, 811, 857, 866
Kzo9I GATC 4 cut(s) 396, 599, 801, 859
LmnI GCTCC 2 cut(s) 17, 169
Lsp1109I GCAGC 2 cut(s) 908, 930
MaeI CTAG 3 cut(s) 173, 323, 983
MaeII ACGT 2 cut(s) 123, 370
MaeIII GTNAC 1 cut(s) 419
MalI GATC 4 cut(s) 398, 601, 803, 861
MboI GATC 4 cut(s) 396, 599, 801, 859
MboII GAAGA 4 cut(s) 222, 348, 502, 567
MflI RGATCY 1 cut(s) 801
MhlI GDGCHC 1 cut(s) 727
MluCI AATT 4 cut(s) 641, 686, 842, 925
MnlI CCTC 7 cut(s) 160, 203, 281, 385, 571, 580, 831
Mph1103I ATGCAT 1 cut(s) 675
MseI TTAA 3 cut(s) 555, 789, 956
MslI CAYNNNNRTG 1 cut(s) 272
MspA1I CMGCKG 1 cut(s) 921
MspI CCGG 1 cut(s) 761
MspR9I CCNGG 2 cut(s) 462, 901
MvaI CCWGG 2 cut(s) 462, 901
MvnI CGCG 3 cut(s) 21, 91, 115
MwoI GCNNNNNNNGC 1 cut(s) 18
NdeII GATC 4 cut(s) 396, 599, 801, 859
NlaIII CATG 6 cut(s) 277, 548, 677, 811, 857, 866
NlaIV GGNNCC 2 cut(s) 525, 591
NruI TCGCGA 1 cut(s) 115
NsiI ATGCAT 1 cut(s) 675
NspI RCATGY 1 cut(s) 677
PagI TCATGA 1 cut(s) 862
PfeI GAWTC 4 cut(s) 227, 379, 466, 628
PkrI GCNGC 3 cut(s) 90, 920, 923
Psp6I CCWGG 2 cut(s) 460, 899
PspGI CCWGG 2 cut(s) 460, 899
PspN4I GGNNCC 2 cut(s) 525, 591
PsuI RGATCY 1 cut(s) 801
PvuII CAGCTG 1 cut(s) 921
RruI TCGCGA 1 cut(s) 115
RsaI GTAC 2 cut(s) 239, 851
RsaNI GTAC 2 cut(s) 238, 850
RseI CAYNNNNRTG 1 cut(s) 272
SaqAI TTAA 3 cut(s) 555, 789, 956
SatI GCNGC 3 cut(s) 89, 919, 922
Sau3AI GATC 4 cut(s) 396, 599, 801, 859
ScrFI CCNGG 2 cut(s) 462, 901
SduI GDGCHC 1 cut(s) 727
SfcI CTRYAG 1 cut(s) 327
SmiMI CAYNNNNRTG 1 cut(s) 272
SmlI CTYRAG 2 cut(s) 728, 797
SmoI CTYRAG 2 cut(s) 728, 797
Sse9I AATT 4 cut(s) 641, 686, 842, 925
SsiI CCGC 3 cut(s) 19, 61, 89
SspI AATATT 2 cut(s) 496, 871
SspMI CTAG 3 cut(s) 173, 323, 983
StyD4I CCNGG 2 cut(s) 460, 899
TaaI ACNGT 1 cut(s) 520
TaiI ACGT 2 cut(s) 126, 373
TaqI TCGA 4 cut(s) 201, 249, 891, 908
TasI AATT 4 cut(s) 641, 686, 842, 925
TatI WGTACW 1 cut(s) 237
TauI GCSGC 1 cut(s) 91
TfiI GAWTC 4 cut(s) 227, 379, 466, 628
Tru1I TTAA 3 cut(s) 555, 789, 956
Tru9I TTAA 3 cut(s) 555, 789, 956
TscAI CASTG 4 cut(s) 63, 192, 525, 727
TseI GCWGC 2 cut(s) 918, 921
TspDTI ATGAA 4 cut(s) 65, 561, 654, 824
TspRI CASTG 4 cut(s) 63, 192, 525, 727
XceI RCATGY 1 cut(s) 677
XspI CTAG 3 cut(s) 173, 323, 983
ZraI GACGTC 1 cut(s) 124
Zsp2I ATGCAT 1 cut(s) 675
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.