Rmu_sc0000367.1_g000109

Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000367.1
OG0006048
Physical Location & Seq
Forward (+)
470124 .. 473666
3543 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1074 bp
atgatggttcttgacttgaggacacgcgcgtgttggggcagcagggcagcagcggcaacactgggcagcagtgcgatggtgagcaatagcagggacttgacgttgtggtgtgcaaggcgtagggctgcagcaggaggcaggcagcagcagggtgcagtgacagggaggctaggtgcgcaggggctgcggcaggatgctgcagcaggaggcttgcggctaggggtgcaggtagggttttcagcagtttttgttgactgtgggcaacttctcattgcctcctttcaaacttgtggaagcatatcactattttacctgagacattgtctagtcttggccgggccggaagatgaaattaatgtagaaaaagtctcaaaaatgttgaagctacccagcctattagttttggtttgcagcattgttcttgttttgcattcataccaagcaacctcacatgatgaacatgatgaagttgaggatgagagggagtttaattatgacgacaacagtgagaaggggccagctagatggggaaagatacgccatgaatggagcatatgtaacaatggatcgttgcagtctcccatagatctgttagatgacagagttgatgtagtctcagatttagggaaacttcagggtaactacagggcttgcaatgccactcttaagaatagaggccatgatatgatgttgaaatggcatgctgatgcaggatatattccaatcaatggaactcagtatacagtcagacaatgccattggcactcaccttctgaacacaccatcaatggcaagcagtttgatttagaggtgcatctggttcatgacagtccaaatggaagcatcgctgttatcgggatcctgtataagtttggaaaacctgacccatttttgacacggatgatggactacttagaagacttatctgatagcagtaaagaagagaaagtgattggtatggttgatccaaagcaaataaaaactgtcagtacaaagtactacagatatataggctctcttacaactcctccttgcacacaaaatgtcctctggaccattgttggagaggtttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0008150 GO:0010033 GO:0010037 GO:0016020 GO:0042221 GO:0042493 GO:0050896 GO:0072347 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko00910 map00910
Pfam Domains
Protein Families
Carb_anhydrase

Protein Analysis

357

Amino Acids

39.54

Weight (kDa)

6.46

Isoelectric Point (pI)

37.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 172 - 355 6.8e-34 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 177
Acc36I ACCTGC 1 cut(s) 217
AccB7I CCANNNNNTGG 1 cut(s) 728
AccI GTMKAC 1 cut(s) 740
AccII CGCG 2 cut(s) 27, 29
AciI CCGC 3 cut(s) 53, 187, 214
AclWI GGATC 4 cut(s) 574, 853, 866, 959
AcoI YGGCCR 1 cut(s) 333
AcuI CTGAAG 1 cut(s) 617
AfaI GTAC 2 cut(s) 991, 998
AfiI CCNNNNNNNGG 1 cut(s) 728
AflII CTTAAG 1 cut(s) 665
AgsI TTSAA 3 cut(s) 284, 382, 694
AleI CACNNNNGTG 1 cut(s) 28
AluBI AGCT 2 cut(s) 385, 521
AluI AGCT 2 cut(s) 385, 521
Alw26I GTCTC 4 cut(s) 310, 373, 582, 619
AlwI GGATC 4 cut(s) 574, 853, 866, 959
AlwNI CAGNNNCTG 1 cut(s) 184
AoxI GGCC 4 cut(s) 333, 338, 515, 676
AseI ATTAAT 1 cut(s) 354
AspLEI GCGC 2 cut(s) 29, 178
AspS9I GGNCC 3 cut(s) 338, 515, 1053
AsuC2I CCSGG 1 cut(s) 337
AsuHPI GGTGA 2 cut(s) 91, 759
AvaII GGWCC 1 cut(s) 1053
BamHI GGATCC 1 cut(s) 858
BbsI GAAGAC 1 cut(s) 924
BccI CCATC 4 cut(s) 70, 519, 791, 898
BcnI CCSGG 1 cut(s) 337
BcoDI GTCTC 4 cut(s) 310, 373, 582, 619
BfaI CTAG 4 cut(s) 170, 218, 326, 522
BfmI CTRYAG 4 cut(s) 126, 198, 643, 1000
BfrI CTTAAG 1 cut(s) 665
BfuAI ACCTGC 1 cut(s) 217
BglII AGATCT 1 cut(s) 586
BmcAI AGTACT 1 cut(s) 998
Bme1390I CCNGG 1 cut(s) 337
Bme18I GGWCC 1 cut(s) 1053
BmgT120I GGNCC 3 cut(s) 338, 515, 1053
BmiI GGNNCC 2 cut(s) 516, 860
BmrFI CCNGG 1 cut(s) 337
BmrI ACTGGG 1 cut(s) 71
BmsI GCATC 4 cut(s) 184, 697, 823, 852
BmuI ACTGGG 1 cut(s) 71
BpiI GAAGAC 1 cut(s) 924
BpuEI CTTGAG 1 cut(s) 37
BpuMI CCSGG 1 cut(s) 337
BsaXI ACNNNNNCTCC 6 cut(s) 157, 187, 541, 571, 1012, 1042
Bsc4I CCNNNNNNNGG 1 cut(s) 728
Bse1I ACTGG 1 cut(s) 66
Bse3DI GCAATG 2 cut(s) 270, 661
BseGI GGATG 3 cut(s) 199, 481, 906
BseLI CCNNNNNNNGG 1 cut(s) 728
BseMI GCAATG 2 cut(s) 270, 661
BseMII CTCAG 3 cut(s) 305, 630, 749
BseNI ACTGG 1 cut(s) 66
BseRI GAGGAG 1 cut(s) 1017
BseYI CCCAGC 1 cut(s) 389
BsgI GTGCAG 2 cut(s) 174, 245
Bsh1236I CGCG 2 cut(s) 27, 29
BshFI GGCC 4 cut(s) 335, 340, 517, 678
BsiSI CCGG 2 cut(s) 336, 341
BslFI GGGAC 1 cut(s) 107
BslI CCNNNNNNNGG 1 cut(s) 728
BsmAI GTCTC 4 cut(s) 310, 373, 582, 619
BsmFI GGGAC 1 cut(s) 107
BsmI GAATGC 1 cut(s) 430
BsnI GGCC 4 cut(s) 335, 340, 517, 678
Bsp143I GATC 4 cut(s) 566, 586, 858, 964
BspACI CCGC 3 cut(s) 53, 187, 214
BspANI GGCC 4 cut(s) 335, 340, 517, 678
BspCNI CTCAG 3 cut(s) 306, 629, 748
BspFNI CGCG 2 cut(s) 27, 29
BspHI TCATGA 1 cut(s) 823
BspLI GGNNCC 2 cut(s) 516, 860
BspMAI CTGCAG 2 cut(s) 130, 202
BspMI ACCTGC 1 cut(s) 217
BspPI GGATC 4 cut(s) 574, 853, 866, 959
BspTI CTTAAG 1 cut(s) 665
BsrDI GCAATG 2 cut(s) 270, 661
BsrI ACTGG 1 cut(s) 66
BssMI GATC 4 cut(s) 566, 586, 858, 964
BssNAI GTATAC 1 cut(s) 741
Bst1107I GTATAC 1 cut(s) 741
Bst4CI ACNGT 5 cut(s) 257, 506, 745, 830, 985
Bst6I CTCTTC 1 cut(s) 936
BstAFI CTTAAG 1 cut(s) 665
BstAPI GCANNNNNTGC 1 cut(s) 184
BstC8I GCNNGC 6 cut(s) 140, 212, 519, 652, 702, 794
BstDEI CTNAG 4 cut(s) 314, 616, 735, 913
BstF5I GGATG 3 cut(s) 199, 481, 906
BstFNI CGCG 2 cut(s) 27, 29
BstHHI GCGC 2 cut(s) 29, 178
BstKTI GATC 4 cut(s) 569, 589, 861, 967
BstMAI GTCTC 4 cut(s) 310, 373, 582, 619
BstMBI GATC 4 cut(s) 566, 586, 858, 964
BstMWI GCNNNNNNNGC 5 cut(s) 53, 175, 184, 223, 656
BstNSI RCATGY 1 cut(s) 704
BstSCI CCNGG 1 cut(s) 335
BstSFI CTRYAG 4 cut(s) 126, 198, 643, 1000
BstUI CGCG 2 cut(s) 27, 29
BstV2I GAAGAC 1 cut(s) 924
BstX2I RGATCY 2 cut(s) 586, 858
BstXI CCANNNNNNTGG 1 cut(s) 525
BstYI RGATCY 2 cut(s) 586, 858
BstZ17I GTATAC 1 cut(s) 741
BsuRI GGCC 4 cut(s) 335, 340, 517, 678
BtgZI GCGATG 2 cut(s) 89, 829
BtsCI GGATG 3 cut(s) 199, 481, 906
BtsI GCAGTG 2 cut(s) 76, 162
BtsIMutI CAGTG 4 cut(s) 59, 76, 162, 511
BveI ACCTGC 1 cut(s) 217
Cac8I GCNNGC 6 cut(s) 140, 212, 519, 652, 702, 794
CaiI CAGNNNCTG 1 cut(s) 184
CciI TCATGA 1 cut(s) 823
CfoI GCGC 2 cut(s) 29, 178
Cfr13I GGNCC 3 cut(s) 338, 515, 1053
Csp6I GTAC 2 cut(s) 990, 997
CviAII CATG 6 cut(s) 452, 461, 542, 680, 701, 824
CviQI GTAC 2 cut(s) 990, 997
DdeI CTNAG 4 cut(s) 314, 616, 735, 913
DpnI GATC 4 cut(s) 568, 588, 860, 966
DpnII GATC 4 cut(s) 566, 586, 858, 964
EaeI YGGCCR 1 cut(s) 333
Eam1104I CTCTTC 1 cut(s) 936
EarI CTCTTC 1 cut(s) 936
Eco47I GGWCC 1 cut(s) 1053
Eco57I CTGAAG 1 cut(s) 617
FaeI CATG 6 cut(s) 455, 464, 545, 683, 704, 827
FaqI GGGAC 1 cut(s) 107
FatI CATG 6 cut(s) 451, 460, 541, 679, 700, 823
FauNDI CATATG 1 cut(s) 554
FblI GTMKAC 1 cut(s) 740
FokI GGATG 3 cut(s) 206, 488, 913
FspBI CTAG 4 cut(s) 170, 218, 326, 522
FspI TGCGCA 1 cut(s) 177
GlaI GCGC 2 cut(s) 28, 177
GsaI CCCAGC 1 cut(s) 393
HaeIII GGCC 4 cut(s) 335, 340, 517, 678
HapII CCGG 2 cut(s) 336, 341
HhaI GCGC 2 cut(s) 29, 178
Hin1II CATG 6 cut(s) 455, 464, 545, 683, 704, 827
Hin6I GCGC 2 cut(s) 27, 176
HinP1I GCGC 2 cut(s) 27, 176
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HpaII CCGG 2 cut(s) 336, 341
HphI GGTGA 2 cut(s) 91, 759
Hpy166II GTNNAC 2 cut(s) 253, 741
Hpy188I TCNGA 4 cut(s) 619, 749, 775, 928
Hpy188III TCNNGA 4 cut(s) 11, 824, 856, 1051
Hpy8I GTNNAC 2 cut(s) 253, 741
HpyAV CCTTC 2 cut(s) 505, 780
HpyCH4III ACNGT 5 cut(s) 257, 506, 745, 830, 985
HpyCH4IV ACGT 1 cut(s) 101
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 175, 184, 223, 656
HpyF3I CTNAG 4 cut(s) 314, 616, 735, 913
HpySE526I ACGT 1 cut(s) 101
Hsp92II CATG 6 cut(s) 455, 464, 545, 683, 704, 827
HspAI GCGC 2 cut(s) 27, 176
Kzo9I GATC 4 cut(s) 566, 586, 858, 964
LmnI GCTCC 1 cut(s) 549
LweI GCATC 4 cut(s) 184, 697, 823, 852
MaeI CTAG 4 cut(s) 170, 218, 326, 522
MaeII ACGT 1 cut(s) 101
MaeIII GTNAC 3 cut(s) 157, 557, 638
MalI GATC 4 cut(s) 568, 588, 860, 966
MboI GATC 4 cut(s) 566, 586, 858, 964
MboII GAAGA 3 cut(s) 356, 929, 953
MflI RGATCY 2 cut(s) 586, 858
MluCI AATT 2 cut(s) 351, 490
MmeI TCCRAC 1 cut(s) 1042
MseI TTAA 4 cut(s) 354, 489, 666, 1072
MslI CAYNNNNRTG 2 cut(s) 28, 705
MspA1I CMGCKG 1 cut(s) 53
MspCI CTTAAG 1 cut(s) 665
MspI CCGG 2 cut(s) 336, 341
MspR9I CCNGG 1 cut(s) 337
Mva1269I GAATGC 1 cut(s) 430
MvnI CGCG 2 cut(s) 27, 29
MwoI GCNNNNNNNGC 5 cut(s) 53, 175, 184, 223, 656
NciI CCSGG 1 cut(s) 337
NdeI CATATG 1 cut(s) 554
NdeII GATC 4 cut(s) 566, 586, 858, 964
NlaIII CATG 6 cut(s) 455, 464, 545, 683, 704, 827
NlaIV GGNNCC 2 cut(s) 516, 860
NmuCI GTSAC 1 cut(s) 157
NsbI TGCGCA 1 cut(s) 177
NspI RCATGY 1 cut(s) 704
OliI CACNNNNGTG 1 cut(s) 28
PaeI GCATGC 1 cut(s) 704
PagI TCATGA 1 cut(s) 823
PctI GAATGC 1 cut(s) 430
PflFI GACNNNGTC 1 cut(s) 321
PflMI CCANNNNNTGG 1 cut(s) 728
PshBI ATTAAT 1 cut(s) 354
PspFI CCCAGC 1 cut(s) 389
PspN4I GGNNCC 2 cut(s) 516, 860
PspPI GGNCC 3 cut(s) 338, 515, 1053
PsrI GAACNNNNNNTAC 2 cut(s) 724, 756
PstI CTGCAG 2 cut(s) 130, 202
PstNI CAGNNNCTG 1 cut(s) 184
PsuI RGATCY 2 cut(s) 586, 858
PsyI GACNNNGTC 1 cut(s) 321
RsaI GTAC 2 cut(s) 991, 998
RsaNI GTAC 2 cut(s) 990, 997
RseI CAYNNNNRTG 2 cut(s) 28, 705
SaqAI TTAA 4 cut(s) 354, 489, 666, 1072
Sau3AI GATC 4 cut(s) 566, 586, 858, 964
Sau96I GGNCC 3 cut(s) 338, 515, 1053
ScaI AGTACT 1 cut(s) 998
ScrFI CCNGG 1 cut(s) 337
SfaNI GCATC 4 cut(s) 184, 697, 823, 852
SfcI CTRYAG 4 cut(s) 126, 198, 643, 1000
SinI GGWCC 1 cut(s) 1053
SmiMI CAYNNNNRTG 2 cut(s) 28, 705
SmlI CTYRAG 2 cut(s) 16, 665
SmoI CTYRAG 2 cut(s) 16, 665
SphI GCATGC 1 cut(s) 704
Sse9I AATT 2 cut(s) 351, 490
SsiI CCGC 3 cut(s) 53, 187, 214
SspMI CTAG 4 cut(s) 170, 218, 326, 522
StyD4I CCNGG 1 cut(s) 335
TaaI ACNGT 5 cut(s) 257, 506, 745, 830, 985
TaiI ACGT 1 cut(s) 104
TasI AATT 2 cut(s) 351, 490
TatI WGTACW 2 cut(s) 989, 996
TauI GCSGC 3 cut(s) 56, 190, 217
Tru1I TTAA 4 cut(s) 354, 489, 666, 1072
Tru9I TTAA 4 cut(s) 354, 489, 666, 1072
TscAI CASTG 4 cut(s) 66, 76, 162, 511
TseFI GTSAC 1 cut(s) 157
Tsp45I GTSAC 1 cut(s) 157
TspDTI ATGAA 6 cut(s) 363, 423, 471, 480, 558, 812
TspGWI ACGGA 1 cut(s) 913
TspRI CASTG 4 cut(s) 66, 76, 162, 511
Tth111I GACNNNGTC 1 cut(s) 321
Van91I CCANNNNNTGG 1 cut(s) 728
Vha464I CTTAAG 1 cut(s) 665
VpaK11BI GGWCC 1 cut(s) 1053
VspI ATTAAT 1 cut(s) 354
XceI RCATGY 1 cut(s) 704
XmiI GTMKAC 1 cut(s) 740
XspI CTAG 4 cut(s) 170, 218, 326, 522
ZrmI AGTACT 1 cut(s) 998
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.