Rmu_sc0000469.1_g000016

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000469.1
OG0004014
Physical Location & Seq
Forward (+)
72796 .. 74166
1371 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 798 bp
atggaagccgaagctcaagaatcgagcccccaaaacgacgctgtcatcacctcattctgcgagatcacctccgcctccaaacaagaagccctcttcttcctcgaatcccacaattacgaccttgacgccgccgtttccactttcttggacaacgaaaacgccgccgctgacgatgacgccgccgctgtcaccgtcaccaacccggtccagccctcccactcgctctcgccgtctccgtcacactcgccctcccagtcgcccatgcgctctcgctccccggcgccagctctgtctcgccaggcctaccagctccggtccaaacgcgccgcctccgggaccagcaaggaggacaacaagccgccggctgcgcgtacacgtggcggcattagcacgctttctgatctgaatcggcttccggtgaagaatgggtctgacagtgactccgatgagcccaacgagtactacactggtggcgagaaaagtggtatgcttgtccaagaccctaccagaggcgggggtgacgtggattcaattttcactcaagccagacagtttgcacaaggacctctggaaccttcgtcaagctcaaaaagttttactggaacagctagaacacttacaggagagaccgttccaactgctgcacctcagccacctgagtccatcaatcacaccattactttctggaggaatggcttttccattacttccaagaaggctgcatttgcaggatcatccagcctccgcagcggaccgtcatctatggaaaagccattcctccagaaagtaatggtgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000096 GO:0000902 GO:0000904 GO:0002682 GO:0002683 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006082 GO:0006139 GO:0006520 GO:0006534 GO:0006555 GO:0006575 GO:0006725 GO:0006732 GO:0006790 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0009058 GO:0009066 GO:0009069 GO:0009116 GO:0009119 GO:0009653 GO:0009759 GO:0009888 GO:0009966 GO:0009968 GO:0009987 GO:0010026 GO:0010090 GO:0010646 GO:0010648 GO:0016043 GO:0016143 GO:0016144 GO:0017144 GO:0018130 GO:0019222 GO:0019438 GO:0019748 GO:0019752 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0023051 GO:0023057 GO:0030154 GO:0031323 GO:0031347 GO:0031348 GO:0032502 GO:0032989 GO:0033353 GO:0034641 GO:0042278 GO:0042343 GO:0042430 GO:0042435 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0045088 GO:0045824 GO:0046128 GO:0046439 GO:0046483 GO:0046498 GO:0046500 GO:0048468 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048856 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051186 GO:0055086 GO:0065007 GO:0071704 GO:0071840 GO:0072521 GO:0080134 GO:0080183 GO:0090342 GO:0090558 GO:0090626 GO:0097159 GO:1900457 GO:1900458 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901657 GO:1901659 GO:2000377
KEGG Pathways
Metabolic & Signaling
ko03015 ko04071 ko04113 ko04114 ko04151 ko04152 ko04261 ko04728 ko05165 map03015 map04071 map04113 map04114 map04151 map04152 map04261 map04728 map05165
Pfam Domains
Protein Families
B56

Protein Analysis

265

Amino Acids

28.11

Weight (kDa)

5.29

Isoelectric Point (pI)

65.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UBA_4 PF14555 13 - 53 4.1e-12 UBA-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 280
AccII CGCG 2 cut(s) 324, 370
AclWI GGATC 1 cut(s) 739
AcvI CACGTG 1 cut(s) 377
AcyI GRCGYC 3 cut(s) 126, 177, 281
AfaI GTAC 2 cut(s) 373, 461
AfiI CCNNNNNNNGG 3 cut(s) 333, 509, 513
AflIII ACRYGT 1 cut(s) 374
AgsI TTSAA 1 cut(s) 531
AjiI CACGTC 1 cut(s) 523
AjnI CCWGG 1 cut(s) 297
AloI GAACNNNNNNTCC 2 cut(s) 615, 647
AluBI AGCT 5 cut(s) 14, 287, 310, 585, 608
AluI AGCT 5 cut(s) 14, 287, 310, 585, 608
Alw26I GTCTC 3 cut(s) 237, 297, 620
AlwI GGATC 1 cut(s) 739
AoxI GGCC 1 cut(s) 300
ApeKI GCWGC 4 cut(s) 365, 641, 719, 747
AspLEI GCGC 4 cut(s) 267, 283, 326, 370
AspS9I GGNCC 5 cut(s) 205, 315, 336, 563, 752
AsuC2I CCSGG 3 cut(s) 203, 278, 334
AsuHPI GGTGA 6 cut(s) 40, 58, 181, 187, 430, 530
AvaII GGWCC 5 cut(s) 205, 315, 336, 563, 752
BanI GGYRCC 1 cut(s) 280
BanII GRGCYC 2 cut(s) 29, 453
BbrPI CACGTG 1 cut(s) 377
BbvCI CCTCAGC 1 cut(s) 648
BbvI GCAGC 4 cut(s) 352, 628, 706, 759
BccI CCATC 1 cut(s) 671
BceAI ACGGC 2 cut(s) 116, 214
BciT130I CCWGG 1 cut(s) 299
BcnI CCSGG 3 cut(s) 203, 278, 334
BcoDI GTCTC 3 cut(s) 237, 297, 620
BfaI CTAG 1 cut(s) 609
BfoI RGCGCY 1 cut(s) 284
BmcAI AGTACT 1 cut(s) 461
Bme1390I CCNGG 4 cut(s) 203, 278, 299, 334
Bme18I GGWCC 5 cut(s) 205, 315, 336, 563, 752
BmgBI CACGTC 1 cut(s) 523
BmgT120I GGNCC 5 cut(s) 205, 315, 336, 563, 752
BmiI GGNNCC 3 cut(s) 282, 337, 573
BmrFI CCNGG 4 cut(s) 203, 278, 299, 334
BmrI ACTGGG 1 cut(s) 247
BmuI ACTGGG 1 cut(s) 247
BplI GAGNNNNNCTC 2 cut(s) 53, 85
BpmI CTGGAG 2 cut(s) 706, 764
Bpu10I CCTNAGC 1 cut(s) 648
BpuEI CTTGAG 1 cut(s) 525
BpuMI CCSGG 3 cut(s) 203, 278, 334
BsaAI YACGTR 1 cut(s) 377
BsaBI GATNNNNATC 1 cut(s) 405
BsaHI GRCGYC 3 cut(s) 126, 177, 281
BsaI GGTCTC 1 cut(s) 620
BsaJI CCNNGG 1 cut(s) 276
BsaWI WCCGGW 2 cut(s) 312, 415
BsaXI ACNNNNNCTCC 6 cut(s) 233, 263, 425, 455, 615, 645
Bsc4I CCNNNNNNNGG 3 cut(s) 333, 509, 513
Bse118I RCCGGY 1 cut(s) 361
Bse1I ACTGG 3 cut(s) 253, 472, 604
Bse8I GATNNNNATC 1 cut(s) 405
BseBI CCWGG 1 cut(s) 299
BseDI CCNNGG 1 cut(s) 276
BseGI GGATG 1 cut(s) 734
BseJI GATNNNNATC 1 cut(s) 405
BseLI CCNNNNNNNGG 3 cut(s) 333, 509, 513
BseMII CTCAG 2 cut(s) 648, 662
BseNI ACTGG 3 cut(s) 253, 472, 604
BseXI GCAGC 4 cut(s) 352, 628, 706, 759
BsgI GTGCAG 1 cut(s) 627
Bsh1236I CGCG 2 cut(s) 324, 370
BshFI GGCC 1 cut(s) 302
BshNI GGYRCC 1 cut(s) 280
BsiSI CCGG 6 cut(s) 203, 278, 313, 333, 362, 416
BslFI GGGAC 1 cut(s) 349
BslI CCNNNNNNNGG 3 cut(s) 333, 509, 513
BsmAI GTCTC 3 cut(s) 237, 297, 620
BsmBI CGTCTC 1 cut(s) 237
BsmFI GGGAC 1 cut(s) 349
BsnI GGCC 1 cut(s) 302
Bso31I GGTCTC 1 cut(s) 620
Bsp1286I GDGCHC 2 cut(s) 29, 453
Bsp143I GATC 3 cut(s) 63, 400, 731
BspANI GGCC 1 cut(s) 302
BspCNI CTCAG 2 cut(s) 649, 661
BspFNI CGCG 2 cut(s) 324, 370
BspLI GGNNCC 3 cut(s) 282, 337, 573
BspPI GGATC 1 cut(s) 739
BspT107I GGYRCC 1 cut(s) 280
BspTNI GGTCTC 1 cut(s) 620
BsrFI RCCGGY 1 cut(s) 361
BsrI ACTGG 3 cut(s) 253, 472, 604
BssAI RCCGGY 1 cut(s) 361
BssECI CCNNGG 1 cut(s) 276
BssMI GATC 3 cut(s) 63, 400, 731
BssNI GRCGYC 3 cut(s) 126, 177, 281
Bst2UI CCWGG 1 cut(s) 299
Bst4CI ACNGT 5 cut(s) 193, 437, 552, 631, 756
Bst6I CTCTTC 1 cut(s) 98
BstACI GRCGYC 3 cut(s) 126, 177, 281
BstBAI YACGTR 1 cut(s) 377
BstC8I GCNNGC 3 cut(s) 285, 363, 392
BstDEI CTNAG 2 cut(s) 648, 657
BstENI CCTNNNNNAGG 1 cut(s) 507
BstF5I GGATG 1 cut(s) 734
BstFNI CGCG 2 cut(s) 324, 370
BstH2I RGCGCY 1 cut(s) 284
BstHHI GCGC 4 cut(s) 267, 283, 326, 370
BstKTI GATC 3 cut(s) 66, 403, 734
BstMAI GTCTC 3 cut(s) 237, 297, 620
BstMBI GATC 3 cut(s) 63, 400, 731
BstMWI GCNNNNNNNGC 4 cut(s) 367, 387, 725, 747
BstNI CCWGG 1 cut(s) 299
BstSCI CCNGG 4 cut(s) 201, 276, 297, 332
BstUI CGCG 2 cut(s) 324, 370
BstV1I GCAGC 4 cut(s) 352, 628, 706, 759
BstXI CCANNNNNNTGG 1 cut(s) 145
BsuRI GGCC 1 cut(s) 302
BtrI CACGTC 1 cut(s) 523
BtsCI GGATG 1 cut(s) 734
BtsIMutI CAGTG 2 cut(s) 442, 465
Cac8I GCNNGC 3 cut(s) 285, 363, 392
CfoI GCGC 4 cut(s) 267, 283, 326, 370
Cfr10I RCCGGY 1 cut(s) 361
Cfr13I GGNCC 5 cut(s) 205, 315, 336, 563, 752
CpoI CGGWCCG 1 cut(s) 752
CseI GACGC 3 cut(s) 47, 134, 185
Csp6I GTAC 2 cut(s) 372, 460
CspI CGGWCCG 1 cut(s) 752
CviAII CATG 1 cut(s) 262
CviQI GTAC 2 cut(s) 372, 460
DdeI CTNAG 2 cut(s) 648, 657
DinI GGCGCC 1 cut(s) 282
DpnI GATC 3 cut(s) 65, 402, 733
DpnII GATC 3 cut(s) 63, 400, 731
Eam1104I CTCTTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 98
EciI GGCGGA 1 cut(s) 61
Eco147I AGGCCT 1 cut(s) 302
Eco24I GRGCYC 2 cut(s) 29, 453
Eco31I GGTCTC 1 cut(s) 620
Eco47I GGWCC 5 cut(s) 205, 315, 336, 563, 752
Eco72I CACGTG 1 cut(s) 377
EcoNI CCTNNNNNAGG 1 cut(s) 507
EcoO109I RGGNCCY 1 cut(s) 563
EcoRII CCWGG 1 cut(s) 297
EcoT38I GRGCYC 2 cut(s) 29, 453
EgeI GGCGCC 1 cut(s) 282
EheI GGCGCC 1 cut(s) 282
Esp3I CGTCTC 1 cut(s) 237
FaeI CATG 1 cut(s) 265
FaiI YATR 3 cut(s) 263, 488, 764
FaqI GGGAC 1 cut(s) 349
FatI CATG 1 cut(s) 261
FauI CCCGC 1 cut(s) 506
FokI GGATG 1 cut(s) 721
FriOI GRGCYC 2 cut(s) 29, 453
FspBI CTAG 1 cut(s) 609
GlaI GCGC 4 cut(s) 266, 282, 325, 369
GsuI CTGGAG 2 cut(s) 706, 764
HaeII RGCGCY 1 cut(s) 284
HaeIII GGCC 1 cut(s) 302
HapII CCGG 6 cut(s) 203, 278, 313, 333, 362, 416
HgaI GACGC 3 cut(s) 47, 134, 185
HhaI GCGC 4 cut(s) 267, 283, 326, 370
Hin1I GRCGYC 3 cut(s) 126, 177, 281
Hin1II CATG 1 cut(s) 265
Hin6I GCGC 4 cut(s) 265, 281, 324, 368
HinP1I GCGC 4 cut(s) 265, 281, 324, 368
HinfI GANTC 6 cut(s) 20, 104, 406, 440, 527, 659
HpaII CCGG 6 cut(s) 203, 278, 313, 333, 362, 416
HphI GGTGA 6 cut(s) 40, 58, 181, 187, 430, 530
Hpy166II GTNNAC 1 cut(s) 374
Hpy188I TCNGA 4 cut(s) 400, 405, 433, 445
Hpy188III TCNNGA 4 cut(s) 17, 569, 685, 781
Hpy8I GTNNAC 1 cut(s) 374
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 2 cut(s) 585, 709
HpyCH4III ACNGT 5 cut(s) 193, 437, 552, 631, 756
HpyCH4IV ACGT 2 cut(s) 376, 522
HpyCH4V TGCA 4 cut(s) 557, 644, 722, 728
HpyF10VI GCNNNNNNNGC 4 cut(s) 367, 387, 725, 747
HpyF3I CTNAG 2 cut(s) 648, 657
HpySE526I ACGT 2 cut(s) 376, 522
Hsp92I GRCGYC 3 cut(s) 126, 177, 281
Hsp92II CATG 1 cut(s) 265
HspAI GCGC 4 cut(s) 265, 281, 324, 368
KasI GGCGCC 1 cut(s) 280
KroI GCCGGC 1 cut(s) 361
KroNI GCCGGC 1 cut(s) 363
Kzo9I GATC 3 cut(s) 63, 400, 731
LmnI GCTCC 2 cut(s) 278, 315
Lsp1109I GCAGC 4 cut(s) 352, 628, 706, 759
MaeI CTAG 1 cut(s) 609
MaeII ACGT 2 cut(s) 376, 522
MaeIII GTNAC 5 cut(s) 187, 193, 237, 437, 518
MalI GATC 3 cut(s) 65, 402, 733
MboI GATC 3 cut(s) 63, 400, 731
MboII GAAGA 3 cut(s) 85, 88, 433
MhlI GDGCHC 2 cut(s) 29, 453
MluCI AATT 2 cut(s) 112, 531
Mly113I GGCGCC 1 cut(s) 281
MlyI GAGTC 2 cut(s) 434, 668
MmeI TCCRAC 1 cut(s) 659
MroNI GCCGGC 1 cut(s) 361
MspA1I CMGCKG 3 cut(s) 167, 185, 750
MspI CCGG 6 cut(s) 203, 278, 313, 333, 362, 416
MspR9I CCNGG 4 cut(s) 203, 278, 299, 334
MvaI CCWGG 1 cut(s) 299
MvnI CGCG 2 cut(s) 324, 370
MwoI GCNNNNNNNGC 4 cut(s) 367, 387, 725, 747
NaeI GCCGGC 1 cut(s) 363
NarI GGCGCC 1 cut(s) 281
NciI CCSGG 3 cut(s) 203, 278, 334
NdeII GATC 3 cut(s) 63, 400, 731
NgoMIV GCCGGC 1 cut(s) 361
NlaIII CATG 1 cut(s) 265
NlaIV GGNNCC 3 cut(s) 282, 337, 573
NmuCI GTSAC 5 cut(s) 187, 193, 237, 437, 518
PceI AGGCCT 1 cut(s) 302
PcsI WCGNNNNNNNCGW 2 cut(s) 227, 233
PdiI GCCGGC 1 cut(s) 363
PfeI GAWTC 4 cut(s) 20, 104, 406, 527
PflFI GACNNNGTC 1 cut(s) 41
PfoI TCCNGGA 1 cut(s) 332
PleI GAGTC 2 cut(s) 434, 667
PluTI GGCGCC 1 cut(s) 284
PmaCI CACGTG 1 cut(s) 377
PmlI CACGTG 1 cut(s) 377
PpsI GAGTC 2 cut(s) 434, 667
Ppu21I YACGTR 1 cut(s) 377
PpuMI RGGWCCY 1 cut(s) 563
Psp5II RGGWCCY 1 cut(s) 563
Psp6I CCWGG 1 cut(s) 297
PspCI CACGTG 1 cut(s) 377
PspGI CCWGG 1 cut(s) 297
PspN4I GGNNCC 3 cut(s) 282, 337, 573
PspPI GGNCC 5 cut(s) 205, 315, 336, 563, 752
PspPPI RGGWCCY 1 cut(s) 563
PsyI GACNNNGTC 1 cut(s) 41
RsaI GTAC 2 cut(s) 373, 461
RsaNI GTAC 2 cut(s) 372, 460
Rsr2I CGGWCCG 1 cut(s) 752
RsrII CGGWCCG 1 cut(s) 752
Sau3AI GATC 3 cut(s) 63, 400, 731
Sau96I GGNCC 5 cut(s) 205, 315, 336, 563, 752
ScaI AGTACT 1 cut(s) 461
SchI GAGTC 2 cut(s) 434, 668
ScrFI CCNGG 4 cut(s) 203, 278, 299, 334
SduI GDGCHC 2 cut(s) 29, 453
SfoI GGCGCC 1 cut(s) 282
SinI GGWCC 5 cut(s) 205, 315, 336, 563, 752
SmlI CTYRAG 2 cut(s) 15, 540
SmoI CTYRAG 2 cut(s) 15, 540
Sse9I AATT 2 cut(s) 112, 531
SseBI AGGCCT 1 cut(s) 302
SspDI GGCGCC 1 cut(s) 280
SspMI CTAG 1 cut(s) 609
StuI AGGCCT 1 cut(s) 302
StyD4I CCNGG 4 cut(s) 201, 276, 297, 332
TaaI ACNGT 5 cut(s) 193, 437, 552, 631, 756
TaiI ACGT 2 cut(s) 379, 525
TaqI TCGA 2 cut(s) 23, 102
TasI AATT 2 cut(s) 112, 531
TatI WGTACW 1 cut(s) 459
TauI GCSGC 8 cut(s) 131, 164, 167, 182, 185, 329, 361, 384
TfiI GAWTC 4 cut(s) 20, 104, 406, 527
TscAI CASTG 2 cut(s) 442, 472
TseFI GTSAC 5 cut(s) 187, 193, 237, 437, 518
TseI GCWGC 4 cut(s) 365, 641, 719, 747
Tsp45I GTSAC 5 cut(s) 187, 193, 237, 437, 518
TspGWI ACGGA 1 cut(s) 225
TspRI CASTG 2 cut(s) 442, 472
Tth111I GACNNNGTC 1 cut(s) 41
VpaK11BI GGWCC 5 cut(s) 205, 315, 336, 563, 752
XagI CCTNNNNNAGG 1 cut(s) 507
XspI CTAG 1 cut(s) 609
ZrmI AGTACT 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.