Rmu_sc0000469.1_g000017

The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000469.1
OG0004014
Physical Location & Seq
Forward (+)
75709 .. 77080
1372 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 606 bp
atgttgtttggttgttccagtggtatgcttgtccaagaccccaccagaggcgggggtgacgtggattcaattttcactcaagccagacagtttgcacaaggacctttggaaccttcgtcaagctcaaaaagttttactggaacagctagaacacttacaggagagaccgttccaactgctgcacctcagccacctgagtccatcaatcacaccattactttctggaggaatggcttttccatagatgacggtccgctgcggaggctggatgatcctgcaaatgcagccttcttggaaagcatcaagaagtctgagtgcccaaaagagcttgaaccagctagtaggaggaccactgttcatgtcagccttatgaagcgggaggaagactaccctgaaccattgaagcagcggggtcaggttgcttttcagggtgttggaagaacattaggtggcagcagctcggagtcagctgcctctgagtcaactgctgctgccagtcctcctcttacaactgctccattgccatcaatgggcttaactgttgatcagtcattgccgtcgacctctattcagctaaggttggctgatggtacccgattgagttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000096 GO:0000902 GO:0000904 GO:0002682 GO:0002683 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005829 GO:0006082 GO:0006139 GO:0006520 GO:0006534 GO:0006555 GO:0006575 GO:0006725 GO:0006732 GO:0006790 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0009058 GO:0009066 GO:0009069 GO:0009116 GO:0009119 GO:0009653 GO:0009759 GO:0009888 GO:0009966 GO:0009968 GO:0009987 GO:0010026 GO:0010090 GO:0010646 GO:0010648 GO:0016043 GO:0016143 GO:0016144 GO:0017144 GO:0018130 GO:0019222 GO:0019438 GO:0019748 GO:0019752 GO:0019757 GO:0019758 GO:0019760 GO:0019761 GO:0023051 GO:0023057 GO:0030154 GO:0031323 GO:0031347 GO:0031348 GO:0032502 GO:0032989 GO:0033353 GO:0034641 GO:0042278 GO:0042343 GO:0042430 GO:0042435 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044272 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0044550 GO:0045088 GO:0045824 GO:0046128 GO:0046439 GO:0046483 GO:0046498 GO:0046500 GO:0048468 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048856 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051186 GO:0055086 GO:0065007 GO:0071704 GO:0071840 GO:0072521 GO:0080134 GO:0080183 GO:0090342 GO:0090558 GO:0090626 GO:0097159 GO:1900457 GO:1900458 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901657 GO:1901659 GO:2000377
KEGG Pathways
Metabolic & Signaling
ko03015 ko04071 ko04113 ko04114 ko04151 ko04152 ko04261 ko04728 ko05165 map03015 map04071 map04113 map04114 map04151 map04152 map04261 map04728 map05165
Pfam Domains
Protein Families
B56

Protein Analysis

201

Amino Acids

21.29

Weight (kDa)

5.45

Isoelectric Point (pI)

60.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEP PF08059 72 - 147 3.3e-27 SEP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 590
AccB1I GGYRCC 1 cut(s) 590
AccI GTMKAC 1 cut(s) 560
AciI CCGC 5 cut(s) 51, 254, 259, 376, 409
AclWI GGATC 1 cut(s) 266
AfaI GTAC 1 cut(s) 592
AfiI CCNNNNNNNGG 3 cut(s) 47, 51, 530
AgsI TTSAA 3 cut(s) 69, 332, 403
AjiI CACGTC 1 cut(s) 61
AloI GAACNNNNNNTCC 2 cut(s) 153, 185
AluBI AGCT 7 cut(s) 123, 146, 328, 338, 459, 470, 574
AluI AGCT 7 cut(s) 123, 146, 328, 338, 459, 470, 574
Alw26I GTCTC 1 cut(s) 158
AlwI GGATC 1 cut(s) 266
ApeKI GCWGC 9 cut(s) 179, 256, 284, 406, 453, 456, 470, 488, 491
Asp718I GGTACC 1 cut(s) 590
AspS9I GGNCC 3 cut(s) 101, 251, 348
AsuHPI GGTGA 1 cut(s) 68
AvaII GGWCC 3 cut(s) 101, 251, 348
BaeGI GKGCMC 1 cut(s) 320
BanI GGYRCC 1 cut(s) 590
BbsI GAAGAC 1 cut(s) 390
BbvCI CCTCAGC 1 cut(s) 186
BbvI GCAGC 9 cut(s) 166, 243, 296, 418, 457, 465, 468, 475, 478
BccI CCATC 3 cut(s) 209, 532, 581
BceAI ACGGC 1 cut(s) 541
BclI TGATCA 1 cut(s) 544
BcoDI GTCTC 1 cut(s) 158
BfaI CTAG 2 cut(s) 147, 339
BisI GCNGC 9 cut(s) 180, 257, 285, 407, 454, 457, 471, 489, 492
BlsI GCNGC 9 cut(s) 181, 258, 286, 408, 455, 458, 472, 490, 493
Bme18I GGWCC 3 cut(s) 101, 251, 348
BmgBI CACGTC 1 cut(s) 61
BmgT120I GGNCC 3 cut(s) 101, 251, 348
BmiI GGNNCC 2 cut(s) 111, 592
BmsI GCATC 1 cut(s) 309
BpiI GAAGAC 1 cut(s) 390
BpmI CTGGAG 1 cut(s) 244
Bpu10I CCTNAGC 2 cut(s) 186, 575
BpuEI CTTGAG 1 cut(s) 63
BsaI GGTCTC 1 cut(s) 158
BsaXI ACNNNNNCTCC 4 cut(s) 153, 183, 499, 529
Bsc4I CCNNNNNNNGG 3 cut(s) 47, 51, 530
Bse1I ACTGG 3 cut(s) 18, 142, 495
Bse3DI GCAATG 2 cut(s) 518, 551
BseGI GGATG 1 cut(s) 274
BseLI CCNNNNNNNGG 3 cut(s) 47, 51, 530
BseMI GCAATG 2 cut(s) 518, 551
BseMII CTCAG 4 cut(s) 186, 200, 303, 468
BseNI ACTGG 3 cut(s) 18, 142, 495
BseRI GAGGAG 1 cut(s) 492
BseSI GKGCMC 1 cut(s) 320
BseXI GCAGC 9 cut(s) 166, 243, 296, 418, 457, 465, 468, 475, 478
BsgI GTGCAG 1 cut(s) 165
BshNI GGYRCC 1 cut(s) 590
BslI CCNNNNNNNGG 3 cut(s) 47, 51, 530
BsmAI GTCTC 1 cut(s) 158
Bso31I GGTCTC 1 cut(s) 158
Bsp1286I GDGCHC 1 cut(s) 320
Bsp143I GATC 2 cut(s) 271, 544
BspACI CCGC 5 cut(s) 51, 254, 259, 376, 409
BspCNI CTCAG 4 cut(s) 187, 199, 304, 469
BspLI GGNNCC 2 cut(s) 111, 592
BspPI GGATC 1 cut(s) 266
BspT107I GGYRCC 1 cut(s) 590
BspTNI GGTCTC 1 cut(s) 158
BsrDI GCAATG 2 cut(s) 518, 551
BsrI ACTGG 3 cut(s) 18, 142, 495
BssMI GATC 2 cut(s) 271, 544
Bst4CI ACNGT 5 cut(s) 90, 169, 251, 355, 541
BstDEI CTNAG 5 cut(s) 186, 195, 312, 477, 575
BstF5I GGATG 1 cut(s) 274
BstKTI GATC 2 cut(s) 274, 547
BstMAI GTCTC 1 cut(s) 158
BstMBI GATC 2 cut(s) 271, 544
BstMWI GCNNNNNNNGC 2 cut(s) 262, 284
BstSLI GKGCMC 1 cut(s) 320
BstV1I GCAGC 9 cut(s) 166, 243, 296, 418, 457, 465, 468, 475, 478
BstV2I GAAGAC 1 cut(s) 390
BtrI CACGTC 1 cut(s) 61
BtsCI GGATG 1 cut(s) 274
BtsIMutI CAGTG 2 cut(s) 25, 351
Cfr13I GGNCC 3 cut(s) 101, 251, 348
CpoI CGGWCCG 1 cut(s) 251
Csp6I GTAC 1 cut(s) 591
CspI CGGWCCG 1 cut(s) 251
CviAII CATG 1 cut(s) 359
CviQI GTAC 1 cut(s) 591
DdeI CTNAG 5 cut(s) 186, 195, 312, 477, 575
DpnI GATC 2 cut(s) 273, 546
DpnII GATC 2 cut(s) 271, 544
Eco31I GGTCTC 1 cut(s) 158
Eco47I GGWCC 3 cut(s) 101, 251, 348
EcoO109I RGGNCCY 1 cut(s) 101
FaeI CATG 1 cut(s) 362
FaiI YATR 4 cut(s) 26, 242, 360, 371
FatI CATG 1 cut(s) 358
FauI CCCGC 3 cut(s) 44, 369, 402
FbaI TGATCA 1 cut(s) 544
FblI GTMKAC 1 cut(s) 560
Fnu4HI GCNGC 9 cut(s) 180, 257, 285, 407, 454, 457, 471, 489, 492
FokI GGATG 1 cut(s) 281
Fsp4HI GCNGC 9 cut(s) 180, 257, 285, 407, 454, 457, 471, 489, 492
FspBI CTAG 2 cut(s) 147, 339
GluI GCNGC 9 cut(s) 180, 257, 285, 407, 454, 457, 471, 489, 492
GsuI CTGGAG 1 cut(s) 244
Hin1II CATG 1 cut(s) 362
HincII GTYRAC 2 cut(s) 483, 561
HindII GTYRAC 2 cut(s) 483, 561
HinfI GANTC 4 cut(s) 65, 197, 464, 479
HphI GGTGA 1 cut(s) 68
Hpy166II GTNNAC 2 cut(s) 483, 561
Hpy188I TCNGA 3 cut(s) 313, 463, 478
Hpy188III TCNNGA 2 cut(s) 223, 304
Hpy8I GTNNAC 2 cut(s) 483, 561
Hpy99I CGWCG 1 cut(s) 562
HpyAV CCTTC 2 cut(s) 123, 298
HpyCH4III ACNGT 5 cut(s) 90, 169, 251, 355, 541
HpyCH4IV ACGT 1 cut(s) 60
HpyCH4V TGCA 4 cut(s) 95, 182, 278, 284
HpyF10VI GCNNNNNNNGC 2 cut(s) 262, 284
HpyF3I CTNAG 5 cut(s) 186, 195, 312, 477, 575
HpySE526I ACGT 1 cut(s) 60
Hsp92II CATG 1 cut(s) 362
KpnI GGTACC 1 cut(s) 594
Ksp22I TGATCA 1 cut(s) 544
Kzo9I GATC 2 cut(s) 271, 544
LmnI GCTCC 1 cut(s) 520
Lsp1109I GCAGC 9 cut(s) 166, 243, 296, 418, 457, 465, 468, 475, 478
LweI GCATC 1 cut(s) 309
MaeI CTAG 2 cut(s) 147, 339
MaeII ACGT 1 cut(s) 60
MaeIII GTNAC 1 cut(s) 56
MalI GATC 2 cut(s) 273, 546
MboI GATC 2 cut(s) 271, 544
MboII GAAGA 2 cut(s) 395, 450
MhlI GDGCHC 1 cut(s) 320
MluCI AATT 1 cut(s) 69
MlyI GAGTC 3 cut(s) 206, 473, 488
MmeI TCCRAC 2 cut(s) 197, 415
MseI TTAA 2 cut(s) 536, 604
MspA1I CMGCKG 3 cut(s) 256, 409, 470
MwoI GCNNNNNNNGC 2 cut(s) 262, 284
NdeII GATC 2 cut(s) 271, 544
NlaIII CATG 1 cut(s) 362
NlaIV GGNNCC 2 cut(s) 111, 592
NmuCI GTSAC 1 cut(s) 56
PfeI GAWTC 1 cut(s) 65
PkrI GCNGC 9 cut(s) 181, 258, 286, 408, 455, 458, 472, 490, 493
PleI GAGTC 3 cut(s) 205, 472, 487
PpsI GAGTC 3 cut(s) 205, 472, 487
PpuMI RGGWCCY 1 cut(s) 101
Psp5II RGGWCCY 1 cut(s) 101
PspN4I GGNNCC 2 cut(s) 111, 592
PspPI GGNCC 3 cut(s) 101, 251, 348
PspPPI RGGWCCY 1 cut(s) 101
PvuII CAGCTG 1 cut(s) 470
RsaI GTAC 1 cut(s) 592
RsaNI GTAC 1 cut(s) 591
Rsr2I CGGWCCG 1 cut(s) 251
RsrII CGGWCCG 1 cut(s) 251
SalI GTCGAC 1 cut(s) 559
SaqAI TTAA 2 cut(s) 536, 604
SatI GCNGC 9 cut(s) 180, 257, 285, 407, 454, 457, 471, 489, 492
Sau3AI GATC 2 cut(s) 271, 544
Sau96I GGNCC 3 cut(s) 101, 251, 348
SchI GAGTC 3 cut(s) 206, 473, 488
SduI GDGCHC 1 cut(s) 320
SfaNI GCATC 1 cut(s) 309
SinI GGWCC 3 cut(s) 101, 251, 348
SmlI CTYRAG 1 cut(s) 78
SmoI CTYRAG 1 cut(s) 78
Sse9I AATT 1 cut(s) 69
SsiI CCGC 5 cut(s) 51, 254, 259, 376, 409
SspMI CTAG 2 cut(s) 147, 339
TaaI ACNGT 5 cut(s) 90, 169, 251, 355, 541
TaiI ACGT 1 cut(s) 63
TaqI TCGA 1 cut(s) 560
TasI AATT 1 cut(s) 69
TfiI GAWTC 1 cut(s) 65
Tru1I TTAA 2 cut(s) 536, 604
Tru9I TTAA 2 cut(s) 536, 604
TscAI CASTG 2 cut(s) 25, 358
TseFI GTSAC 1 cut(s) 56
TseI GCWGC 9 cut(s) 179, 256, 284, 406, 453, 456, 470, 488, 491
Tsp45I GTSAC 1 cut(s) 56
TspDTI ATGAA 2 cut(s) 347, 386
TspRI CASTG 2 cut(s) 25, 358
VpaK11BI GGWCC 3 cut(s) 101, 251, 348
XmiI GTMKAC 1 cut(s) 560
XspI CTAG 2 cut(s) 147, 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.