Rmu_sc0000483.1_g000007

Non-catalytic subunit of the queuine tRNA- ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, - His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7- deazaguanosine)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000483.1
OG0006935
Physical Location & Seq
Reverse (-)
30164 .. 36616
6453 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1005 bp
atgctgggtttgcgggaccacggattcgtagccattccgagagattctattcaatgcctcccagaatgtaatagtacgaataaattgggtgcatctttcgagactccctgtggtcgcagtttggttaagcctgtagagtatgttgaattgatttcttcgttaaggcctaatatatggagtgctttggctgatgaagttcctgcatgggtgtctgataagaggaacaagacctcggttgatcgaacagtggatctgtttttggatccattgttggaggatcttctgttcaagaacgtgagcgatgtgcacaagaggtggccagccgaaatgtatcaggttaactacagttttctattattttcttctgaaaaaagatgctattggattggaggttttgggcttggagagagcatggatgagcgccctgctctccttaatgctgttacttgtgaagttgtttctcatttttgggagatggtgtttatagaggggttgagtcggaagatcccagcaacgagacttcttttaagtgccaggaataacggcttccgaacagaagaggtcttgcagggtgttgctgcaggaattgatatctttgactctacgtatatataccatcttaccgttggaggctttgcacttaccttcccactagatggtgttgagaccaatctctctgcttttcagttgactaatataggaagtgatcagacaaagattaatttgagagcaacaatgcacaggaaagacacttcaccaattgttgaaaattgtaaatgttacacgtgccagaatcatacaaaagcgtacatcaatcacctgctcaatgttcatgaaatgctagctcagattctacttgaaatgcataatactcaccactatctaagtttcttccgcttagtattggaggcaattaagtctggtaattttgagcaattcagactgaaattttctgagagtaagcgttatcatcttgcttctgaagctgctgttgcctgcatgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TGT

Protein Analysis

334

Amino Acids

37.91

Weight (kDa)

6.18

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TGT PF01702 20 - 320 3.4e-47 Queuine tRNA-ribosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 828
Acc36I ACCTGC 1 cut(s) 828
AccB7I CCANNNNNTGG 1 cut(s) 656
AciI CCGC 2 cut(s) 13, 895
AclWI GGATC 5 cut(s) 257, 258, 270, 285, 499
AcoI YGGCCR 1 cut(s) 317
AcsI RAATTY 1 cut(s) 947
AcuI CTGAAG 1 cut(s) 1002
AcvI CACGTG 1 cut(s) 786
AfaI GTAC 2 cut(s) 76, 809
AfiI CCNNNNNNNGG 1 cut(s) 656
AflIII ACRYGT 1 cut(s) 783
AgsI TTSAA 5 cut(s) 53, 146, 289, 767, 860
AjnI CCWGG 1 cut(s) 533
AloI GAACNNNNNNTCC 2 cut(s) 269, 301
AluBI AGCT 2 cut(s) 845, 986
AluI AGCT 2 cut(s) 845, 986
Alw21I GWGCWC 1 cut(s) 309
Alw26I GTCTC 3 cut(s) 95, 511, 659
Alw44I GTGCAC 1 cut(s) 305
AlwI GGATC 5 cut(s) 257, 258, 270, 285, 499
AoxI GGCC 2 cut(s) 164, 317
ApaLI GTGCAC 1 cut(s) 305
ApeKI GCWGC 2 cut(s) 578, 986
ApoI RAATTY 1 cut(s) 947
ArsI GACNNNNNNTTYG 2 cut(s) 8, 40
AseI ATTAAT 1 cut(s) 720
AspLEI GCGC 1 cut(s) 423
AspS9I GGNCC 1 cut(s) 16
AsuHPI GGTGA 3 cut(s) 747, 809, 866
AsuNHI GCTAGC 1 cut(s) 841
AvaII GGWCC 1 cut(s) 16
BaeGI GKGCMC 1 cut(s) 309
BalI TGGCCA 1 cut(s) 319
BamHI GGATCC 1 cut(s) 262
BbrPI CACGTG 1 cut(s) 786
Bbv12I GWGCWC 1 cut(s) 309
BbvI GCAGC 2 cut(s) 565, 973
BccI CCATC 3 cut(s) 469, 624, 650
BceAI ACGGC 1 cut(s) 559
BciT130I CCWGG 1 cut(s) 535
BclI TGATCA 1 cut(s) 706
BcoDI GTCTC 3 cut(s) 95, 511, 659
BfaI CTAG 2 cut(s) 653, 842
BfmI CTRYAG 3 cut(s) 132, 343, 579
BfoI RGCGCY 1 cut(s) 424
BfuAI ACCTGC 1 cut(s) 828
BisI GCNGC 2 cut(s) 579, 987
BlsI GCNGC 2 cut(s) 580, 988
Bme1390I CCNGG 1 cut(s) 535
Bme18I GGWCC 1 cut(s) 16
BmgT120I GGNCC 1 cut(s) 16
BmiI GGNNCC 2 cut(s) 17, 264
BmrFI CCNGG 1 cut(s) 535
BmsI GCATC 2 cut(s) 101, 365
BmtI GCTAGC 1 cut(s) 845
BsaAI YACGTR 2 cut(s) 606, 786
BsaI GGTCTC 1 cut(s) 659
BsaJI CCNNGG 2 cut(s) 19, 231
BsaXI ACNNNNNCTCC 2 cut(s) 464, 494
Bsc4I CCNNNNNNNGG 1 cut(s) 656
BseBI CCWGG 1 cut(s) 535
BseDI CCNNGG 2 cut(s) 19, 231
BseGI GGATG 1 cut(s) 421
BseLI CCNNNNNNNGG 1 cut(s) 656
BseMII CTCAG 2 cut(s) 860, 945
BseSI GKGCMC 1 cut(s) 309
BseXI GCAGC 2 cut(s) 565, 973
BseYI CCCAGC 2 cut(s) 4, 508
BshFI GGCC 2 cut(s) 166, 319
BsiHKAI GWGCWC 1 cut(s) 309
BslFI GGGAC 1 cut(s) 29
BslI CCNNNNNNNGG 1 cut(s) 656
BsmAI GTCTC 3 cut(s) 95, 511, 659
BsmFI GGGAC 1 cut(s) 29
BsnI GGCC 2 cut(s) 166, 319
Bso31I GGTCTC 1 cut(s) 659
Bsp1286I GDGCHC 1 cut(s) 309
Bsp143I GATC 6 cut(s) 238, 250, 262, 277, 504, 706
BspACI CCGC 2 cut(s) 13, 895
BspANI GGCC 2 cut(s) 166, 319
BspCNI CTCAG 2 cut(s) 859, 946
BspHI TCATGA 1 cut(s) 832
BspLI GGNNCC 2 cut(s) 17, 264
BspMAI CTGCAG 1 cut(s) 583
BspMI ACCTGC 1 cut(s) 828
BspOI GCTAGC 1 cut(s) 845
BspPI GGATC 5 cut(s) 257, 258, 270, 285, 499
BspTNI GGTCTC 1 cut(s) 659
BssECI CCNNGG 2 cut(s) 19, 231
BssMI GATC 6 cut(s) 238, 250, 262, 277, 504, 706
Bst2UI CCWGG 1 cut(s) 535
Bst4CI ACNGT 3 cut(s) 247, 347, 625
Bst6I CTCTTC 1 cut(s) 552
BstBAI YACGTR 2 cut(s) 606, 786
BstC8I GCNNGC 3 cut(s) 321, 843, 997
BstDEI CTNAG 4 cut(s) 846, 884, 898, 954
BstDSI CCRYGG 1 cut(s) 19
BstF5I GGATG 1 cut(s) 421
BstH2I RGCGCY 1 cut(s) 424
BstHHI GCGC 1 cut(s) 423
BstKTI GATC 6 cut(s) 241, 253, 265, 280, 507, 709
BstMAI GTCTC 3 cut(s) 95, 511, 659
BstMBI GATC 6 cut(s) 238, 250, 262, 277, 504, 706
BstMWI GCNNNNNNNGC 3 cut(s) 10, 983, 992
BstNI CCWGG 1 cut(s) 535
BstNSI RCATGY 1 cut(s) 1003
BstSCI CCNGG 1 cut(s) 533
BstSFI CTRYAG 3 cut(s) 132, 343, 579
BstSLI GKGCMC 1 cut(s) 309
BstSNI TACGTA 1 cut(s) 606
BstV1I GCAGC 2 cut(s) 565, 973
BstX2I RGATCY 4 cut(s) 250, 262, 277, 504
BstYI RGATCY 4 cut(s) 250, 262, 277, 504
BsuRI GGCC 2 cut(s) 166, 319
BtgI CCRYGG 1 cut(s) 19
BtgZI GCGATG 1 cut(s) 315
BtsCI GGATG 1 cut(s) 421
BtsIMutI CAGTG 1 cut(s) 252
BveI ACCTGC 1 cut(s) 828
Cac8I GCNNGC 3 cut(s) 321, 843, 997
CciI TCATGA 1 cut(s) 832
CfoI GCGC 1 cut(s) 423
Cfr13I GGNCC 1 cut(s) 16
Csp6I GTAC 2 cut(s) 75, 808
CviAII CATG 4 cut(s) 204, 412, 833, 1000
CviQI GTAC 2 cut(s) 75, 808
DdeI CTNAG 4 cut(s) 846, 884, 898, 954
DpnI GATC 6 cut(s) 240, 252, 264, 279, 506, 708
DpnII GATC 6 cut(s) 238, 250, 262, 277, 504, 706
EaeI YGGCCR 1 cut(s) 317
Eam1104I CTCTTC 1 cut(s) 552
EarI CTCTTC 1 cut(s) 552
Eco105I TACGTA 1 cut(s) 606
Eco147I AGGCCT 1 cut(s) 166
Eco31I GGTCTC 1 cut(s) 659
Eco32I GATATC 1 cut(s) 592
Eco47I GGWCC 1 cut(s) 16
Eco57I CTGAAG 1 cut(s) 1002
Eco72I CACGTG 1 cut(s) 786
EcoRII CCWGG 1 cut(s) 533
EcoRV GATATC 1 cut(s) 592
EcoT22I ATGCAT 1 cut(s) 867
FaeI CATG 4 cut(s) 207, 415, 836, 1003
FaqI GGGAC 1 cut(s) 29
FatI CATG 4 cut(s) 203, 411, 832, 999
FauI CCCGC 1 cut(s) 6
FbaI TGATCA 1 cut(s) 706
Fnu4HI GCNGC 2 cut(s) 579, 987
FokI GGATG 1 cut(s) 428
Fsp4HI GCNGC 2 cut(s) 579, 987
FspBI CTAG 2 cut(s) 653, 842
GlaI GCGC 1 cut(s) 422
GluI GCNGC 2 cut(s) 579, 987
GsaI CCCAGC 2 cut(s) 8, 512
HaeII RGCGCY 1 cut(s) 424
HaeIII GGCC 2 cut(s) 166, 319
HhaI GCGC 1 cut(s) 423
Hin1II CATG 4 cut(s) 207, 415, 836, 1003
Hin6I GCGC 1 cut(s) 421
HinP1I GCGC 1 cut(s) 421
HincII GTYRAC 2 cut(s) 340, 690
HindII GTYRAC 2 cut(s) 340, 690
HinfI GANTC 7 cut(s) 24, 44, 103, 496, 599, 793, 850
HpaI GTTAAC 1 cut(s) 340
HphI GGTGA 3 cut(s) 747, 809, 866
Hpy166II GTNNAC 3 cut(s) 307, 340, 690
Hpy188III TCNNGA 3 cut(s) 100, 289, 833
Hpy8I GTNNAC 3 cut(s) 307, 340, 690
HpyAV CCTTC 1 cut(s) 655
HpyCH4III ACNGT 3 cut(s) 247, 347, 625
HpyCH4IV ACGT 3 cut(s) 294, 605, 785
HpyCH4V TGCA 9 cut(s) 92, 203, 307, 568, 581, 638, 739, 865, 999
HpyF10VI GCNNNNNNNGC 3 cut(s) 10, 983, 992
HpyF3I CTNAG 4 cut(s) 846, 884, 898, 954
HpySE526I ACGT 3 cut(s) 294, 605, 785
Hsp92II CATG 4 cut(s) 207, 415, 836, 1003
HspAI GCGC 1 cut(s) 421
Ksp22I TGATCA 1 cut(s) 706
KspAI GTTAAC 1 cut(s) 340
Kzo9I GATC 6 cut(s) 238, 250, 262, 277, 504, 706
Lsp1109I GCAGC 2 cut(s) 565, 973
LweI GCATC 2 cut(s) 101, 365
MaeI CTAG 2 cut(s) 653, 842
MaeII ACGT 3 cut(s) 294, 605, 785
MaeIII GTNAC 2 cut(s) 442, 779
MalI GATC 6 cut(s) 240, 252, 264, 279, 506, 708
MboI GATC 6 cut(s) 238, 250, 262, 277, 504, 706
MboII GAAGA 6 cut(s) 147, 272, 354, 514, 569, 883
MfeI CAATTG 1 cut(s) 759
MflI RGATCY 4 cut(s) 250, 262, 277, 504
MhlI GDGCHC 1 cut(s) 309
MlsI TGGCCA 1 cut(s) 319
MluNI TGGCCA 1 cut(s) 319
MlyI GAGTC 3 cut(s) 97, 505, 593
MmeI TCCRAC 3 cut(s) 252, 479, 607
Mox20I TGGCCA 1 cut(s) 319
Mph1103I ATGCAT 1 cut(s) 867
MscI TGGCCA 1 cut(s) 319
MseI TTAA 7 cut(s) 126, 161, 339, 435, 527, 720, 915
Msp20I TGGCCA 1 cut(s) 319
MspR9I CCNGG 1 cut(s) 535
MunI CAATTG 1 cut(s) 759
MvaI CCWGG 1 cut(s) 535
MwoI GCNNNNNNNGC 3 cut(s) 10, 983, 992
NdeII GATC 6 cut(s) 238, 250, 262, 277, 504, 706
NheI GCTAGC 1 cut(s) 841
NlaIII CATG 4 cut(s) 207, 415, 836, 1003
NlaIV GGNNCC 2 cut(s) 17, 264
NsiI ATGCAT 1 cut(s) 867
NspI RCATGY 1 cut(s) 1003
PagI TCATGA 1 cut(s) 832
PaqCI CACCTGC 1 cut(s) 828
PceI AGGCCT 1 cut(s) 166
PfeI GAWTC 4 cut(s) 24, 44, 793, 850
PflMI CCANNNNNTGG 1 cut(s) 656
PkrI GCNGC 2 cut(s) 580, 988
PleI GAGTC 3 cut(s) 97, 504, 593
PmaCI CACGTG 1 cut(s) 786
PmlI CACGTG 1 cut(s) 786
PpsI GAGTC 3 cut(s) 97, 504, 593
Ppu21I YACGTR 2 cut(s) 606, 786
PshBI ATTAAT 1 cut(s) 720
Psp6I CCWGG 1 cut(s) 533
PspCI CACGTG 1 cut(s) 786
PspFI CCCAGC 2 cut(s) 4, 508
PspGI CCWGG 1 cut(s) 533
PspN4I GGNNCC 2 cut(s) 17, 264
PspPI GGNCC 1 cut(s) 16
PstI CTGCAG 1 cut(s) 583
PsuI RGATCY 4 cut(s) 250, 262, 277, 504
RsaI GTAC 2 cut(s) 76, 809
RsaNI GTAC 2 cut(s) 75, 808
SaqAI TTAA 7 cut(s) 126, 161, 339, 435, 527, 720, 915
SatI GCNGC 2 cut(s) 579, 987
Sau3AI GATC 6 cut(s) 238, 250, 262, 277, 504, 706
Sau96I GGNCC 1 cut(s) 16
SchI GAGTC 3 cut(s) 97, 505, 593
ScrFI CCNGG 1 cut(s) 535
SduI GDGCHC 1 cut(s) 309
SfaNI GCATC 2 cut(s) 101, 365
SfcI CTRYAG 3 cut(s) 132, 343, 579
SinI GGWCC 1 cut(s) 16
SnaBI TACGTA 1 cut(s) 606
SseBI AGGCCT 1 cut(s) 166
SsiI CCGC 2 cut(s) 13, 895
SspMI CTAG 2 cut(s) 653, 842
StuI AGGCCT 1 cut(s) 166
StyD4I CCNGG 1 cut(s) 533
TaaI ACNGT 3 cut(s) 247, 347, 625
TaiI ACGT 3 cut(s) 297, 608, 788
TaqI TCGA 2 cut(s) 99, 241
TfiI GAWTC 4 cut(s) 24, 44, 793, 850
Tru1I TTAA 7 cut(s) 126, 161, 339, 435, 527, 720, 915
Tru9I TTAA 7 cut(s) 126, 161, 339, 435, 527, 720, 915
TscAI CASTG 1 cut(s) 252
TseI GCWGC 2 cut(s) 578, 986
TspDTI ATGAA 3 cut(s) 207, 821, 849
TspGWI ACGGA 1 cut(s) 36
TspRI CASTG 1 cut(s) 252
Van91I CCANNNNNTGG 1 cut(s) 656
VneI GTGCAC 1 cut(s) 305
VpaK11BI GGWCC 1 cut(s) 16
VspI ATTAAT 1 cut(s) 720
XapI RAATTY 1 cut(s) 947
XceI RCATGY 1 cut(s) 1003
XcmI CCANNNNNNNNNTGG 1 cut(s) 623
XspI CTAG 2 cut(s) 653, 842
Zsp2I ATGCAT 1 cut(s) 867
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.