Rmu_sc0000565.1_g000041

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000565.1
OG0008889
Physical Location & Seq
Forward (+)
171599 .. 175918
4320 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3252 bp
atgacgactcctggaggctcttcttcttgcacccattcatggagtcatgatgtctttctgagttttagaggcacggatacacgcttcaattttacatgccatttgcgcaactatttgcgtcgtaaggggatcaacaccttcatagatgataatgagctcaacagaggagaagaaataactccggctcttctcaaagcaattgaagaatcaaggattgctgtcatagtgttttctgaaaattatgcattctcagagtggtgtctggatgaactcacacatatccttgactgtcggaagtcaaagcaacaaacagtataccctgtgttttacaaagtagatccgtccgatattcaggaccaaaaaggtagttttggtgaggcacttgctcagcatgaatgcagattcaaggacgactcggccaaggtggcgaaatggaaggaagcgctcatagaggcctcaaatttggctggatgggagtacaaggatgggtatgaatcaacattcactgatatcattgttgaacagatctgtaggcaagtgtgttgtcataccgattttatagttgcagattacccagttggaatagaatgtcggctaaagaaaatgcaggatctcttaggtgttgggtcaagtgatgttcgtatgatagggatatgggggactgctggaataggtaagacaacaattgccaaggctgtttataatgcccttgcctgtaagtttgagtgttgctgttttctacaaaatgttggagaagaatcaatgcaacttggtggtccagtcaaactacaaaagatgcttctgtcgaagatcctaagggtgaaagaatccgatctgatgactgaagcctgtgaaggaatgaatgcgataaagagcaagttaagcagcagaaaagttctcttaattctggatgctgtggatcgattggaacagttaaggtatttagttggaaaacccgagtggtttggtttgggcagtagaattatcataacaacaagagataaacgcatgttatctgctcatggtctcggagagaagcaaatatacaagccgaaggagttagatgaacacgaagctttagagctcttcagggcaaatgcctttccacaagaaagcctcccggattgttatgcacaactcgcaaaggatgcattagaatatgctcaaggccttccattagctctgcaatacttgggtacattattatatgatagaactgtagaggaatggaaggaggaattacatagtcgtaggaaatctcttcaggctgatcttcagaagaatctcataacaagttataatgaattggacagttcggtgaagcaagttttccttgacattgcatgtttctttaaaggtaaacataagaatcatgtgatacaaatactagaaaaaagtgacgtaaaacctatgactagtatcaaaattctccaagaaaaggccctcatacatattcaagaaaatcatatgttagaaaatcatatttgtatgcacaacctgctagaggtactgggtaaggaaatagttcacactgaatcaccagagcctgccgaacgcagcagattatggtttttggacgatgtttacaagattctatcagaaggcaaaggaacaaataagatcaaaggtgtcatagtgtttcccagtccacatgagataccattgagtgaaacatgcttctcaggcatgacaaatctcaaatactttattagcagtaatgctgagttttctggaaacattgcatgtctcccaaatgagttgaggcttattgattggccaaggtttccctttgactgtttgccgctgaattatgttcccaaggagcttgttaagctctatatgcctcacagccgtattttacaactaggggagaacttcaagagatcaagaaatttgaaatatataaatctggagaactgccaattccttaaagaaatccccaacttctctggattcccaaatttaagggacttgaatctgaactactgtacaagtttagttaaggttgatgattctgttggaaacctacataaccttgttgctttgagtctttctggatgctacaaccttgttacctttccaacaagaattgccttgacatctgtgaaacatataattttcagagactgcagaagtgtcagggctttgccagtaattgttcaaaacatggaattcttgacatggttggacctatctggtactggtatcgaagagttgcctgatgcgtcaattaggtgcctcataagcctcaaggagttaagcctaagggaatgtgaaaacttaaaaagtctgccatcattcatttatgagttgcgacatctgcagtatcttgatctccacaactgctcaaaacttgcggctctcccagagtggaacgcagaagtgcttccaactaacttgaattcttcacataagttggggactcttaacctgggtggttgcaagcagcttctaaacattcctgaacttccaccaaccgtggaatgggtagatgcagatgattgtgttcaattggaaagatttgcaaaattgtcaaacatcatgaaacataaagagtcaaaaatgatgaaacatatcacattatccaactgtcagagactctgtgacagtctggttcttgatatggcaaagactaaaaatattctaccaagtcaggccagtctatgctcactcttcctctcttgtaggcagtctgaatttgaagttgtatttcctggaagtgaagttccaaaatgtttcagtcatagcaagaagtcgaaggagctgactgaagaatcgaaattttcttttgaaatacctccaaatttcagattgcggaaccaagggttggctatctgtgctgcagctgaagtggttgagataattaacccagaagaacaaaataattgctctgtcacagccacaattgacatcaatgcaggaagcaaaatagcacgttcgtttcattttgaagggaaagtcatggaatcaggtcatgtgtggctgtgctacattccattcattatttttacatgttccctatcacccccttttacttgtggagttactttggaacataaaagcaaaggtgcggtgcggtgcataagttatggggtccacttgattccgttatgccttaaagacttggaatttgaggaggaagttgtgagaattgtaacttggttatgcatcagcggctttgttcagcaatgtatgacagctttgcaaatatttctattttctagcgtttacaaggaggagctgcagaaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

1083

Amino Acids

122.99

Weight (kDa)

6.3

Isoelectric Point (pI)

49.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 16 - 178 5.2e-52 TIR domain
TIR_2 PF13676 18 - 114 3.3e-16 TIR domain
NB-ARC PF00931 201 - 371 3.7e-15 NB-ARC domain
WHD_ROQ1 PF23282 441 - 513 3.2e-11 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 644 - 781 6.8e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 702, 1299
Acc16I TGCGCA 1 cut(s) 107
Acc36I ACCTGC 1 cut(s) 1506
AccB1I GGYRCC 1 cut(s) 2235
AccB7I CCANNNNNTGG 1 cut(s) 2827
AccI GTMKAC 1 cut(s) 315
AciI CCGC 6 cut(s) 1802, 2357, 2815, 3071, 3076, 3174
AclWI GGATC 5 cut(s) 137, 332, 618, 805, 927
AcoI YGGCCR 2 cut(s) 417, 1775
AcuI CTGAAG 6 cut(s) 864, 1072, 1247, 1259, 2790, 2868
AfaI GTAC 5 cut(s) 479, 1198, 1509, 1990, 2200
AfeI AGCGCT 1 cut(s) 444
AfiI CCNNNNNNNGG 5 cut(s) 39, 1438, 1504, 2484, 2827
AflIII ACRYGT 1 cut(s) 3011
AhlI ACTAGT 1 cut(s) 1415
AjnI CCWGG 3 cut(s) 10, 2430, 2713
AjuI GAANNNNNNNTTGG 4 cut(s) 2810, 2842, 3142, 3174
AloI GAACNNNNNNTCC 2 cut(s) 2709, 2741
Alw21I GWGCWC 2 cut(s) 159, 1086
Alw26I GTCTC 4 cut(s) 1031, 1751, 2118, 2590
AlwI GGATC 5 cut(s) 137, 332, 618, 805, 927
AlwNI CAGNNNCTG 2 cut(s) 1547, 2127
Ama87I CYCGRG 1 cut(s) 956
Aor51HI AGCGCT 1 cut(s) 444
AoxI GGCC 6 cut(s) 417, 453, 1168, 1440, 1775, 2655
ApeKI GCWGC 6 cut(s) 885, 1557, 2446, 2840, 2843, 3241
ArsI GACNNNNNNTTYG 2 cut(s) 3113, 3145
Asp700I GAANNNNTTC 2 cut(s) 1524, 2385
AspLEI GCGC 2 cut(s) 108, 445
AspS9I GGNCC 5 cut(s) 355, 776, 1441, 2188, 3094
AsuC2I CCSGG 1 cut(s) 1121
AsuHPI GGTGA 5 cut(s) 386, 832, 1330, 1530, 3015
AvaI CYCGRG 1 cut(s) 956
AvaII GGWCC 4 cut(s) 355, 776, 2188, 3094
AxyI CCTNAGG 2 cut(s) 815, 2264
BalI TGGCCA 1 cut(s) 1777
BanI GGYRCC 1 cut(s) 2235
BanII GRGCYC 2 cut(s) 159, 1086
BarI GAAGNNNNNNTAC 2 cut(s) 1028, 1060
Bbv12I GWGCWC 2 cut(s) 159, 1086
BbvI GCAGC 6 cut(s) 897, 1569, 2458, 2827, 2855, 3228
BccI CCATC 3 cut(s) 465, 479, 2302
BceAI ACGGC 1 cut(s) 1836
BciT130I CCWGG 3 cut(s) 12, 2432, 2715
BciVI GTATCC 1 cut(s) 70
BcnI CCSGG 1 cut(s) 1121
BcoDI GTCTC 4 cut(s) 1031, 1751, 2118, 2590
BcuI ACTAGT 1 cut(s) 1415
BfaI CTAG 5 cut(s) 1388, 1416, 1502, 1865, 3222
BfmI CTRYAG 6 cut(s) 529, 1218, 2128, 2321, 2841, 3242
BfoI RGCGCY 1 cut(s) 446
BfuAI ACCTGC 1 cut(s) 1506
BfuI GTATCC 1 cut(s) 70
BglI GCCNNNNNGGC 1 cut(s) 425
BglII AGATCT 1 cut(s) 525
BisI GCNGC 9 cut(s) 886, 1558, 1802, 2358, 2447, 2841, 2844, 3175, 3242
BlpI GCTNAGC 1 cut(s) 387
BlsI GCNGC 9 cut(s) 887, 1559, 1803, 2359, 2448, 2842, 2845, 3176, 3243
Bme1390I CCNGG 4 cut(s) 12, 1121, 2432, 2715
Bme18I GGWCC 4 cut(s) 355, 776, 2188, 3094
BmeT110I CYCGRG 1 cut(s) 956
BmgT120I GGNCC 5 cut(s) 355, 776, 1441, 2188, 3094
BmiI GGNNCC 3 cut(s) 2237, 2819, 3095
BmrFI CCNGG 4 cut(s) 12, 1121, 2432, 2715
BmrI ACTGGG 3 cut(s) 569, 1520, 1638
BmsI GCATC 7 cut(s) 786, 901, 1138, 2048, 2212, 2482, 3177
BmuI ACTGGG 3 cut(s) 569, 1520, 1638
BpmI CTGGAG 2 cut(s) 33, 1931
Bpu1102I GCTNAGC 1 cut(s) 387
BpuEI CTTGAG 2 cut(s) 1149, 2234
BpuMI CCSGG 1 cut(s) 1121
Bsa29I ATCGAT 1 cut(s) 922
BsaI GGTCTC 1 cut(s) 1031
BsaJI CCNNGG 7 cut(s) 420, 690, 1778, 1818, 2431, 2478, 2821
Bsc4I CCNNNNNNNGG 5 cut(s) 39, 1438, 1504, 2484, 2827
Bse1I ACTGG 7 cut(s) 575, 779, 1515, 1644, 2150, 2206, 2658
Bse21I CCTNAGG 2 cut(s) 815, 2264
Bse3DI GCAATG 3 cut(s) 1338, 1737, 3194
BseBI CCWGG 3 cut(s) 12, 2432, 2715
BseCI ATCGAT 1 cut(s) 922
BseDI CCNNGG 7 cut(s) 420, 690, 1778, 1818, 2431, 2478, 2821
BseGI GGATG 6 cut(s) 271, 476, 490, 916, 1153, 2063
BseLI CCNNNNNNNGG 5 cut(s) 39, 1438, 1504, 2484, 2827
BseMI GCAATG 3 cut(s) 1338, 1737, 3194
BseMII CTCAG 5 cut(s) 50, 264, 401, 1695, 1713
BseNI ACTGG 7 cut(s) 575, 779, 1515, 1644, 2150, 2206, 2658
BseRI GAGGAG 3 cut(s) 180, 3149, 3251
BseXI GCAGC 6 cut(s) 897, 1569, 2458, 2827, 2855, 3228
BshFI GGCC 6 cut(s) 419, 455, 1170, 1442, 1777, 2657
BshNI GGYRCC 1 cut(s) 2235
BshVI ATCGAT 1 cut(s) 922
BsiHKAI GWGCWC 2 cut(s) 159, 1086
BsiHKCI CYCGRG 1 cut(s) 956
BsiSI CCGG 2 cut(s) 182, 1121
BslFI GGGAC 3 cut(s) 673, 1982, 2434
BslI CCNNNNNNNGG 5 cut(s) 39, 1438, 1504, 2484, 2827
BsmAI GTCTC 4 cut(s) 1031, 1751, 2118, 2590
BsmFI GGGAC 3 cut(s) 673, 1982, 2434
BsmI GAATGC 3 cut(s) 245, 401, 868
BsnI GGCC 6 cut(s) 419, 455, 1170, 1442, 1777, 2657
Bso31I GGTCTC 1 cut(s) 1031
BsoBI CYCGRG 1 cut(s) 956
Bsp1286I GDGCHC 2 cut(s) 159, 1086
Bsp1407I TGTACA 1 cut(s) 1988
Bsp1720I GCTNAGC 1 cut(s) 387
BspACI CCGC 6 cut(s) 1802, 2357, 2815, 3071, 3076, 3174
BspANI GGCC 6 cut(s) 419, 455, 1170, 1442, 1777, 2657
BspCNI CTCAG 5 cut(s) 51, 263, 400, 1694, 1714
BspDI ATCGAT 1 cut(s) 922
BspHI TCATGA 2 cut(s) 46, 2541
BspLI GGNNCC 3 cut(s) 2237, 2819, 3095
BspMAI CTGCAG 4 cut(s) 2132, 2325, 2845, 3246
BspMI ACCTGC 1 cut(s) 1506
BspPI GGATC 5 cut(s) 137, 332, 618, 805, 927
BspQI GCTCTTC 3 cut(s) 25, 192, 1091
BspT107I GGYRCC 1 cut(s) 2235
BspTNI GGTCTC 1 cut(s) 1031
BsrDI GCAATG 3 cut(s) 1338, 1737, 3194
BsrGI TGTACA 1 cut(s) 1988
BsrI ACTGG 7 cut(s) 575, 779, 1515, 1644, 2150, 2206, 2658
BssECI CCNNGG 7 cut(s) 420, 690, 1778, 1818, 2431, 2478, 2821
BssNAI GTATAC 1 cut(s) 316
BssT1I CCWWGG 5 cut(s) 420, 690, 1778, 1818, 2821
Bst1107I GTATAC 1 cut(s) 316
Bst2UI CCWGG 3 cut(s) 12, 2432, 2715
Bst6I CTCTTC 6 cut(s) 25, 192, 1091, 1266, 2205, 2678
BstAPI GCANNNNNTGC 2 cut(s) 1148, 1498
BstAUI TGTACA 1 cut(s) 1988
BstC8I GCNNGC 2 cut(s) 1548, 2444
BstDEI CTNAG 8 cut(s) 59, 250, 387, 616, 815, 1681, 1722, 2264
BstDSI CCRYGG 1 cut(s) 2478
BstENI CCTNNNNNAGG 1 cut(s) 1502
BstF5I GGATG 6 cut(s) 271, 476, 490, 916, 1153, 2063
BstH2I RGCGCY 1 cut(s) 446
BstHHI GCGC 2 cut(s) 108, 445
BstMAI GTCTC 4 cut(s) 1031, 1751, 2118, 2590
BstNI CCWGG 3 cut(s) 12, 2432, 2715
BstNSI RCATGY 6 cut(s) 99, 1012, 1347, 1677, 1746, 3015
BstSCI CCNGG 4 cut(s) 10, 1119, 2430, 2713
BstSFI CTRYAG 6 cut(s) 529, 1218, 2128, 2321, 2841, 3242
BstV1I GCAGC 6 cut(s) 897, 1569, 2458, 2827, 2855, 3228
BstX2I RGATCY 4 cut(s) 337, 525, 610, 810
BstYI RGATCY 4 cut(s) 337, 525, 610, 810
BstZ17I GTATAC 1 cut(s) 316
Bsu15I ATCGAT 1 cut(s) 922
Bsu36I CCTNAGG 2 cut(s) 815, 2264
BsuI GTATCC 1 cut(s) 70
BsuRI GGCC 6 cut(s) 419, 455, 1170, 1442, 1777, 2657
BsuTUI ATCGAT 1 cut(s) 922
BtgI CCRYGG 1 cut(s) 2478
BtsCI GGATG 6 cut(s) 271, 476, 490, 916, 1153, 2063
BtsIMutI CAGTG 2 cut(s) 504, 1530
BveI ACCTGC 1 cut(s) 1506
Cac8I GCNNGC 2 cut(s) 1548, 2444
CaiI CAGNNNCTG 2 cut(s) 1547, 2127
CciI TCATGA 2 cut(s) 46, 2541
CfoI GCGC 2 cut(s) 108, 445
Cfr13I GGNCC 5 cut(s) 355, 776, 1441, 2188, 3094
ClaI ATCGAT 1 cut(s) 922
CseI GACGC 2 cut(s) 107, 2214
Csp6I GTAC 5 cut(s) 478, 1197, 1508, 1989, 2199
CviQI GTAC 5 cut(s) 478, 1197, 1508, 1989, 2199
DdeI CTNAG 8 cut(s) 59, 250, 387, 616, 815, 1681, 1722, 2264
DraI TTTAAA 1 cut(s) 1354
EaeI YGGCCR 2 cut(s) 417, 1775
Eam1104I CTCTTC 6 cut(s) 25, 192, 1091, 1266, 2205, 2678
EarI CTCTTC 6 cut(s) 25, 192, 1091, 1266, 2205, 2678
Ecl136II GAGCTC 2 cut(s) 157, 1084
Eco130I CCWWGG 5 cut(s) 420, 690, 1778, 1818, 2821
Eco147I AGGCCT 2 cut(s) 455, 1170
Eco24I GRGCYC 2 cut(s) 159, 1086
Eco31I GGTCTC 1 cut(s) 1031
Eco32I GATATC 1 cut(s) 511
Eco47I GGWCC 4 cut(s) 355, 776, 2188, 3094
Eco47III AGCGCT 1 cut(s) 444
Eco53kI GAGCTC 2 cut(s) 157, 1084
Eco57I CTGAAG 6 cut(s) 864, 1072, 1247, 1259, 2790, 2868
Eco81I CCTNAGG 2 cut(s) 815, 2264
Eco88I CYCGRG 1 cut(s) 956
EcoICRI GAGCTC 2 cut(s) 157, 1084
EcoNI CCTNNNNNAGG 1 cut(s) 1502
EcoO109I RGGNCCY 1 cut(s) 1441
EcoRI GAATTC 2 cut(s) 2171, 2401
EcoRII CCWGG 3 cut(s) 10, 2430, 2713
EcoRV GATATC 1 cut(s) 511
EcoT14I CCWWGG 5 cut(s) 420, 690, 1778, 1818, 2821
EcoT22I ATGCAT 3 cut(s) 247, 1153, 3170
EcoT38I GRGCYC 2 cut(s) 159, 1086
ErhI CCWWGG 5 cut(s) 420, 690, 1778, 1818, 2821
FalI AAGNNNNNCTT 2 cut(s) 1317, 1349
FaqI GGGAC 3 cut(s) 673, 1982, 2434
FauNDI CATATG 1 cut(s) 1467
FblI GTMKAC 1 cut(s) 315
Fnu4HI GCNGC 9 cut(s) 886, 1558, 1802, 2358, 2447, 2841, 2844, 3175, 3242
FokI GGATG 6 cut(s) 278, 483, 497, 923, 1160, 2070
FriOI GRGCYC 2 cut(s) 159, 1086
Fsp4HI GCNGC 9 cut(s) 886, 1558, 1802, 2358, 2447, 2841, 2844, 3175, 3242
FspBI CTAG 5 cut(s) 1388, 1416, 1502, 1865, 3222
FspI TGCGCA 1 cut(s) 107
GlaI GCGC 2 cut(s) 107, 444
GluI GCNGC 9 cut(s) 886, 1558, 1802, 2358, 2447, 2841, 2844, 3175, 3242
GsuI CTGGAG 2 cut(s) 33, 1931
HaeII RGCGCY 1 cut(s) 446
HaeIII GGCC 6 cut(s) 419, 455, 1170, 1442, 1777, 2657
HapII CCGG 2 cut(s) 182, 1121
HgaI GACGC 2 cut(s) 107, 2214
HhaI GCGC 2 cut(s) 108, 445
Hin6I GCGC 2 cut(s) 106, 443
HinP1I GCGC 2 cut(s) 106, 443
HindIII AAGCTT 1 cut(s) 1074
HpaII CCGG 2 cut(s) 182, 1121
HphI GGTGA 5 cut(s) 386, 832, 1330, 1530, 3015
Hpy166II GTNNAC 7 cut(s) 316, 1361, 1528, 1585, 1649, 3097, 3229
Hpy8I GTNNAC 7 cut(s) 316, 1361, 1528, 1585, 1649, 3097, 3229
Hpy99I CGWCG 1 cut(s) 123
HpyAV CCTTC 9 cut(s) 148, 430, 848, 1048, 1181, 1225, 1595, 2752, 2945
HpyCH4IV ACGT 2 cut(s) 1401, 2935
HpyF3I CTNAG 8 cut(s) 59, 250, 387, 616, 815, 1681, 1722, 2264
HpySE526I ACGT 2 cut(s) 1401, 2935
HspAI GCGC 2 cut(s) 106, 443
LguI GCTCTTC 3 cut(s) 25, 192, 1091
LmnI GCTCC 3 cut(s) 1822, 2761, 3238
Lsp1109I GCAGC 6 cut(s) 897, 1569, 2458, 2827, 2855, 3228
LweI GCATC 7 cut(s) 786, 901, 1138, 2048, 2212, 2482, 3177
MaeI CTAG 5 cut(s) 1388, 1416, 1502, 1865, 3222
MaeII ACGT 2 cut(s) 1401, 2935
MaeIII GTNAC 6 cut(s) 1397, 2071, 2603, 2893, 3043, 3154
MfeI CAATTG 4 cut(s) 198, 684, 2510, 2904
MflI RGATCY 4 cut(s) 337, 525, 610, 810
MhlI GDGCHC 2 cut(s) 159, 1086
MlsI TGGCCA 1 cut(s) 1777
MluNI TGGCCA 1 cut(s) 1777
MlyI GAGTC 6 cut(s) 52, 407, 2056, 2416, 2564, 2592
MmeI TCCRAC 9 cut(s) 272, 559, 730, 928, 1999, 2105, 2166, 2414, 2610
Mox20I TGGCCA 1 cut(s) 1777
Mph1103I ATGCAT 3 cut(s) 247, 1153, 3170
MroXI GAANNNNTTC 2 cut(s) 1524, 2385
MscI TGGCCA 1 cut(s) 1777
Msp20I TGGCCA 1 cut(s) 1777
MspA1I CMGCKG 3 cut(s) 1804, 2846, 3174
MspI CCGG 2 cut(s) 182, 1121
MspR9I CCNGG 4 cut(s) 12, 1121, 2432, 2715
MunI CAATTG 4 cut(s) 198, 684, 2510, 2904
Mva1269I GAATGC 3 cut(s) 245, 401, 868
MvaI CCWGG 3 cut(s) 12, 2432, 2715
NciI CCSGG 1 cut(s) 1121
NdeI CATATG 1 cut(s) 1467
NlaIV GGNNCC 3 cut(s) 2237, 2819, 3095
NmeAIII GCCGAG 1 cut(s) 395
NmuCI GTSAC 3 cut(s) 1397, 2603, 2893
NsbI TGCGCA 1 cut(s) 107
NsiI ATGCAT 3 cut(s) 247, 1153, 3170
NspI RCATGY 6 cut(s) 99, 1012, 1347, 1677, 1746, 3015
PagI TCATGA 2 cut(s) 46, 2541
PceI AGGCCT 2 cut(s) 455, 1170
PciI ACATGT 1 cut(s) 3011
PciSI GCTCTTC 3 cut(s) 25, 192, 1091
PctI GAATGC 3 cut(s) 245, 401, 868
PdmI GAANNNNTTC 2 cut(s) 1524, 2385
PflMI CCANNNNNTGG 1 cut(s) 2827
PfoI TCCNGGA 3 cut(s) 10, 1119, 2713
PkrI GCNGC 9 cut(s) 887, 1559, 1803, 2359, 2448, 2842, 2845, 3176, 3243
PleI GAGTC 6 cut(s) 51, 407, 2055, 2416, 2563, 2592
PpsI GAGTC 6 cut(s) 51, 407, 2055, 2416, 2563, 2592
PscI ACATGT 1 cut(s) 3011
PsiI TTATAA 2 cut(s) 702, 1299
Psp124BI GAGCTC 2 cut(s) 159, 1086
Psp6I CCWGG 3 cut(s) 10, 2430, 2713
PspGI CCWGG 3 cut(s) 10, 2430, 2713
PspN4I GGNNCC 3 cut(s) 2237, 2819, 3095
PspPI GGNCC 5 cut(s) 355, 776, 1441, 2188, 3094
PstI CTGCAG 4 cut(s) 2132, 2325, 2845, 3246
PstNI CAGNNNCTG 2 cut(s) 1547, 2127
PsuI RGATCY 4 cut(s) 337, 525, 610, 810
PvuII CAGCTG 1 cut(s) 2846
RsaI GTAC 5 cut(s) 479, 1198, 1509, 1990, 2200
RsaNI GTAC 5 cut(s) 478, 1197, 1508, 1989, 2199
SacI GAGCTC 2 cut(s) 159, 1086
SapI GCTCTTC 3 cut(s) 25, 192, 1091
SatI GCNGC 9 cut(s) 886, 1558, 1802, 2358, 2447, 2841, 2844, 3175, 3242
Sau96I GGNCC 5 cut(s) 355, 776, 1441, 2188, 3094
SchI GAGTC 6 cut(s) 52, 407, 2056, 2416, 2564, 2592
ScrFI CCNGG 4 cut(s) 12, 1121, 2432, 2715
SduI GDGCHC 2 cut(s) 159, 1086
SfaNI GCATC 7 cut(s) 786, 901, 1138, 2048, 2212, 2482, 3177
SfcI CTRYAG 6 cut(s) 529, 1218, 2128, 2321, 2841, 3242
SinI GGWCC 4 cut(s) 355, 776, 2188, 3094
SmlI CTYRAG 2 cut(s) 1164, 2249
SmoI CTYRAG 2 cut(s) 1164, 2249
SpeI ACTAGT 1 cut(s) 1415
SseBI AGGCCT 2 cut(s) 455, 1170
SsiI CCGC 6 cut(s) 1802, 2357, 2815, 3071, 3076, 3174
SspI AATATT 2 cut(s) 2641, 3210
SspMI CTAG 5 cut(s) 1388, 1416, 1502, 1865, 3222
SstI GAGCTC 2 cut(s) 159, 1086
StuI AGGCCT 2 cut(s) 455, 1170
StyD4I CCNGG 4 cut(s) 10, 1119, 2430, 2713
StyI CCWWGG 5 cut(s) 420, 690, 1778, 1818, 2821
TaiI ACGT 2 cut(s) 1404, 2938
TaqI TCGA 5 cut(s) 806, 922, 2208, 2756, 2777
TatI WGTACW 2 cut(s) 477, 1988
TauI GCSGC 3 cut(s) 1804, 2360, 3177
TscAI CASTG 2 cut(s) 511, 1537
TseFI GTSAC 3 cut(s) 1397, 2603, 2893
TseI GCWGC 6 cut(s) 885, 1557, 2446, 2840, 2843, 3241
Tsp45I GTSAC 3 cut(s) 1397, 2603, 2893
TspGWI ACGGA 3 cut(s) 89, 330, 3096
TspRI CASTG 2 cut(s) 511, 1537
Van91I CCANNNNNTGG 1 cut(s) 2827
VpaK11BI GGWCC 4 cut(s) 355, 776, 2188, 3094
XagI CCTNNNNNAGG 1 cut(s) 1502
XceI RCATGY 6 cut(s) 99, 1012, 1347, 1677, 1746, 3015
XcmI CCANNNNNNNNNTGG 1 cut(s) 2481
XmiI GTMKAC 1 cut(s) 315
XmnI GAANNNNTTC 2 cut(s) 1524, 2385
XspI CTAG 5 cut(s) 1388, 1416, 1502, 1865, 3222
Zsp2I ATGCAT 3 cut(s) 247, 1153, 3170
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.